primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	TTTCCTTGAAAGGGGTGCTC	20	20	50.0	50.0	58.2	58.0	20.3	9.2	0.173044	29781	30560	902-921	1.000000	78.9
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	TTTCCTTGAAAGGGGTGCTC	20	20	50.0	50.0	58.2	58.0	20.3	9.2	0.173044	29781	30560	901-920	1.000000	78.9
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCTAGCCGCACTTAAAAGCC	TTTCCTTGAAAGGGGTGCTC	20	20	50.0	50.0	58.3	58.0	0.0	9.2	0.270461	29781	30560	916-935	1.000000	78.1
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCGCACTTAAAAGCCGAGTA	TTTCCTTGAAAGGGGTGCTC	20	20	50.0	50.0	58.3	58.0	5.3	9.2	0.287430	29781	30560	911-930	1.000000	78.0
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TACCGTTGAGTTAGCAACCC	TTTCCTTGAAAGGGGTGCTC	20	20	50.0	50.0	57.5	58.0	0.0	9.2	0.465381	29781	30560	888-907	1.000000	76.5
rps16_p1	rps16	1	ATATACAAGTCGCTACCCCC	TATGGACAGCTGAACCAGTG	20	20	50.0	50.0	56.2	57.2	0.0	29.2	2.577330	30561	31680	1249-1249	1.000000	59.6
rps16_p2	rps16	2	ATATACAAGTCGCTACCCCC	ATGGACAGCTGAACCAGTGA	20	20	50.0	50.0	56.2	58.9	0.0	29.2	2.749979	30561	31680	1248-1248	1.000000	58.2
rps16_p3	rps16	3	ATATACAAGTCGCTACCCCC	GTCTATGGACAGCTGAACCA	20	20	50.0	50.0	56.2	56.9	0.0	28.4	2.868365	30561	31680	1252-1252	1.000000	57.2
rps16_p4	rps16	4	ATATACAAGTCGCTACCCCC	TGTCTATGGACAGCTGAACC	20	20	50.0	50.0	56.2	56.9	0.0	23.3	2.868365	30561	31680	1253-1253	1.000000	57.2
rps16_p5	rps16	5	ATATACAAGTCGCTACCCCC	CTATGGACAGCTGAACCAGT	20	20	50.0	50.0	56.2	56.9	0.0	29.2	2.876787	30561	31680	1250-1250	1.000000	57.2
trnS-GCU-trnS-CGA_p1	trnS-GCU-trnS-CGA	1	CAATCCGACGCTTTAGTCCA	ACCACTAAACTATACCCGCT	20	20	50.0	45.0	58.0	55.6	0.0	0.0	2.358738	33964	34530	622-622	1.000000	61.4
trnS-GCU-trnS-CGA_p2	trnS-GCU-trnS-CGA	2	AATCCGACGCTTTAGTCCAC	ACCACTAAACTATACCCGCT	20	20	50.0	45.0	58.0	55.6	0.0	0.0	2.360126	33964	34530	621-621	1.000000	61.4
trnS-GCU-trnS-CGA_p3	trnS-GCU-trnS-CGA	3	ACGGAAAGAGAGGGATTCGA	ACCACTAAACTATACCCGCT	20	20	50.0	45.0	58.2	55.6	0.0	0.0	2.505503	33964	34530	676-676	1.000000	60.2
trnS-GCU-trnS-CGA_p4	trnS-GCU-trnS-CGA	4	AGCAATCCGACGCTTTAGTC	ACCACTAAACTATACCCGCT	20	20	50.0	45.0	58.4	55.6	0.0	0.0	2.712578	33964	34530	624-624	1.000000	58.6
trnS-GCU-trnS-CGA_p5	trnS-GCU-trnS-CGA	5	CAATCCGACGCTTTAGTCCA	CGAACGAATCACACTTTTACCA	20	22	50.0	40.9	58.0	57.3	0.0	0.0	2.723441	33964	34530	640-640	1.000000	58.5
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	CCCGTCATGTTCCTTGGATT	20	20	45.0	50.0	58.0	57.9	1.4	0.0	0.167157	39053	40038	1104-1120	1.000000	80.0
atpH-atpI_p2	atpH-atpI	2	AGCCAATCCAGCAGCAATAA	CCCGTCATGTTCCTTGGATT	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.277216	39053	40038	1120-1136	1.000000	79.1
atpH-atpI_p3	atpH-atpI	3	AATAACGGAAGCGGCAGAAA	CCCGTCATGTTCCTTGGATT	20	20	45.0	50.0	58.2	57.9	1.4	0.0	0.312696	39053	40038	1105-1121	1.000000	78.8
atpH-atpI_p4	atpH-atpI	4	GCCAATCCAGCAGCAATAAC	CCCGTCATGTTCCTTGGATT	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.343157	39053	40038	1119-1135	1.000000	78.5
atpH-atpI_p5	atpH-atpI	5	TCCAGCAGCAATAACGGAAG	CCCGTCATGTTCCTTGGATT	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.390932	39053	40038	1114-1130	1.000000	78.2
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CATTGCCTCATTTGCATCCC	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.316428	52363	53501	1228-1228	1.000000	77.6
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	GTTCTTCTGTCAAACCCCGA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.331972	52363	53501	1304-1304	1.000000	77.5
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	AGTTCTTCTGTCAAACCCCG	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.7	58.1	0.0	0.0	0.338245	52363	53501	1305-1305	1.000000	77.4
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	CATTGCCTCATTTGCATCCC	TTTGGTAATCAGGCGACACC	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.418225	52363	53501	1247-1247	1.000000	76.8
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	GTTCTTCTGTCAAACCCCGA	TTTGGTAATCAGGCGACACC	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.433769	52363	53501	1323-1323	1.000000	76.7
petN-psbM_p1	petN-psbM	1	ACTCGTAGTGTGGGGAAGAA	TCCTACCGCCTTTCTGCTTA	20	20	50.0	50.0	58.0	58.4	0.0	0.0	0.441446	54500	55296	883-883	1.000000	76.8
petN-psbM_p2	petN-psbM	2	TCGTAGTGTGGGGAAGAAGT	TCCTACCGCCTTTCTGCTTA	20	20	50.0	50.0	58.0	58.4	0.0	0.0	0.441446	54500	55296	881-881	1.000000	76.8
petN-psbM_p3	petN-psbM	3	CGTAGTGTGGGGAAGAAGTG	TCCTACCGCCTTTCTGCTTA	20	20	55.0	50.0	57.9	58.4	0.0	0.0	0.516362	54500	55296	880-880	1.000000	76.2
petN-psbM_p4	petN-psbM	4	ACTCGTAGTGTGGGGAAGAA	CCTACCGCCTTTCTGCTTAT	20	20	50.0	50.0	58.0	57.4	0.0	0.0	0.627434	54500	55296	882-882	1.000000	75.3
petN-psbM_p5	petN-psbM	5	TCGTAGTGTGGGGAAGAAGT	CCTACCGCCTTTCTGCTTAT	20	20	50.0	50.0	58.0	57.4	0.0	0.0	0.627434	54500	55296	880-880	1.000000	75.3
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	TAAGCAGAAAGGCGGTAGGA	TGCGAGATCCCCTATTTCCT	20	20	50.0	50.0	58.4	57.9	0.0	0.0	0.539229	55402	55930	701-701	1.000000	76.0
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	TAAGCAGAAAGGCGGTAGGA	ATTTCCTTTCCCCGGGATTG	20	20	50.0	50.0	58.4	57.8	0.0	26.3	0.595255	55402	55930	688-688	1.000000	75.6
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	ATAAGCAGAAAGGCGGTAGG	TGCGAGATCCCCTATTTCCT	20	20	50.0	50.0	57.4	57.9	0.0	0.0	0.725217	55402	55930	702-702	1.000000	74.5
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	TAAGCAGAAAGGCGGTAGGA	TTCCTTTCCCCGGGATTGTA	20	20	50.0	50.0	58.4	58.3	0.0	26.3	0.764783	55402	55930	686-686	1.000000	74.2
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	TAAGCAGAAAGGCGGTAGGA	TCCCCGGGATTGTAGTTCAA	20	20	50.0	50.0	58.4	58.3	0.0	26.3	0.777054	55402	55930	680-680	1.000000	74.1
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	20	20	55.0	50.0	58.1	58.1	7.6	0.0	0.177181	56995	58183	1349-1350	1.000000	78.8
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	AGTGACTCGTCCTGGAATGA	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.188382	56995	58183	1393-1394	1.000000	78.7
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.199336	56995	58183	1352-1353	1.000000	78.6
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	56995	58183	1339-1340	1.000000	77.7
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	AGTGACTCGTCCTGGAATGA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.321338	56995	58183	1383-1384	1.000000	77.6
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.9	57.1	6.4	0.0	0.992787	89821	90744	1116-1116	1.000000	72.2
psbE-petL_p2	psbE-petL	2	ACTGACGAATAACCAACCCG	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.9	57.1	0.0	0.0	1.030402	89821	90744	1129-1129	1.000000	71.9
psbE-petL_p3	psbE-petL	3	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	24.9	0.0	1.097796	89821	90744	1039-1039	1.000000	71.4
psbE-petL_p4	psbE-petL	4	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	20	20	45.0	50.0	58.2	57.1	0.0	0.0	1.112474	89821	90744	1125-1125	1.000000	71.2
psbE-petL_p5	psbE-petL	5	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	0.0	0.0	1.128804	89821	90744	1124-1124	1.000000	71.1
psbH_p1	psbH	1	TTTCAAGACCCCAACGGATC	AGCCAGGACTTTGTGATTCG	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.380688	99156	99377	339-339	1.000000	77.4
psbH_p2	psbH	2	TCAAGACCCCAACGGATCTA	AGCCAGGACTTTGTGATTCG	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.427767	99156	99377	337-337	1.000000	77.1
psbH_p3	psbH	3	AGACCCCAACGGATCTATCA	AGCCAGGACTTTGTGATTCG	20	20	50.0	50.0	57.5	58.2	0.0	0.0	0.658302	99156	99377	334-334	1.000000	75.2
psbH_p4	psbH	4	TTTCAAGACCCCAACGGATC	GCCAGGACTTTGTGATTCGT	20	20	50.0	50.0	57.8	58.5	0.0	0.0	0.670444	99156	99377	338-338	1.000000	75.1
psbH_p5	psbH	5	TCAAGACCCCAACGGATCTA	GCCAGGACTTTGTGATTCGT	20	20	50.0	50.0	57.8	58.5	0.0	0.0	0.717523	99156	99377	336-336	1.000000	74.7
rpl16_p1	rpl16	1	ATTAATTTTCGAGCCCCGCT	TTCCCCTACAAACCATTCGC	20	20	45.0	50.0	57.9	58.2	0.0	0.0	0.223803	105704	107007	1757-1757	1.000000	78.4
rpl16_p2	rpl16	2	ACCGCTGTCACGTTTAAGTT	TTCCCCTACAAACCATTCGC	20	20	45.0	50.0	58.1	58.2	0.0	0.0	0.226989	105704	107007	1920-1920	1.000000	78.4
rpl16_p3	rpl16	3	ATTAATTTTCGAGCCCCGCT	AATCGAAAGTAGACCGCGAG	20	20	45.0	50.0	57.9	57.8	0.0	8.2	0.243107	105704	107007	2056-2056	1.000000	78.2
rpl16_p4	rpl16	4	ATTAATTTTCGAGCCCCGCT	GGAATTGCCCGCATAGAGAT	20	20	45.0	50.0	57.9	57.8	0.0	0.0	0.243910	105704	107007	2133-2133	1.000000	78.2
rpl16_p5	rpl16	5	ACCGCTGTCACGTTTAAGTT	AATCGAAAGTAGACCGCGAG	20	20	45.0	50.0	58.1	57.8	0.0	8.2	0.246293	105704	107007	2219-2219	1.000000	78.2
rps3_p1	rps3	1	CCCCTTTTTGTTCTATCGACG	ACCCTAGATGGCAAAAGACA	21	20	47.6	45.0	57.0	56.4	0.0	0.0	3.582041	107163	107825	762-762	1.000000	51.6
rps3_p2	rps3	2	CCCTTTTTGTTCTATCGACGG	ACCCTAGATGGCAAAAGACA	21	20	47.6	45.0	57.0	56.4	0.0	0.0	3.582041	107163	107825	761-761	1.000000	51.6
rps3_p3	rps3	3	TGAGTTTCCCCCTTTTTGTTCT	ACCCTAGATGGCAAAAGACA	22	20	40.9	45.0	58.0	56.4	0.0	0.0	3.582252	107163	107825	770-770	1.000000	51.5
rps3_p4	rps3	4	CGGAATTTAGTTGATTGGCCAG	ACCCTAGATGGCAAAAGACA	22	20	45.5	45.0	58.0	56.4	5.1	0.0	3.604011	107163	107825	804-804	1.000000	51.4
rps3_p5	rps3	5	TCCCCCTTTTTGTTCTATCGAC	ACCCTAGATGGCAAAAGACA	22	20	45.5	45.0	58.1	56.4	0.0	0.0	3.624702	107163	107825	764-764	1.000000	51.2
rpl22_p1	rpl22	1	CAAGTCGGAAACCAAGTGGA	TGAATTTCCAAGGACACGCA	20	20	50.0	45.0	58.0	58.0	3.8	0.0	0.063941	107810	108274	592-592	1.000000	79.6
rpl22_p2	rpl22	2	GATGGCTTTGGGTTGTACCA	TGAATTTCCAAGGACACGCA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.107863	107810	108274	610-610	1.000000	79.3
rpl22_p3	rpl22	3	TGATGGCTTTGGGTTGTACC	TGAATTTCCAAGGACACGCA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.107863	107810	108274	611-611	1.000000	79.3
rpl22_p4	rpl22	4	AAGGGAATGATGGCTTTGGG	TGAATTTCCAAGGACACGCA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.155671	107810	108274	618-618	1.000000	78.9
rpl22_p5	rpl22	5	GGGTTGTACCAAGTCGGAAA	TGAATTTCCAAGGACACGCA	20	20	50.0	45.0	57.7	58.0	0.0	0.0	0.285001	107810	108274	601-601	1.000000	77.9
ndhF_p1	ndhF	1	AGACCCGTTTCTGAAGAGTC	AGGTCGGGTGAATTGGAAAC	20	20	50.0	50.0	56.9	58.1	0.0	0.0	1.205697	134182	136404	2354-2355	1.000000	70.8
ndhF_p2	ndhF	2	GACCCGTTTCTGAAGAGTCT	AGGTCGGGTGAATTGGAAAC	20	20	50.0	50.0	56.9	58.1	0.0	0.0	1.205697	134182	136404	2353-2354	1.000000	70.8
ndhF_p3	ndhF	3	AGACCCGTTTCTGAAGAGTC	GAAGGTCGGGTGAATTGGAA	20	20	50.0	50.0	56.9	57.8	0.0	0.0	1.305536	134182	136404	2356-2357	1.000000	70.0
ndhF_p4	ndhF	4	GACCCGTTTCTGAAGAGTCT	GAAGGTCGGGTGAATTGGAA	20	20	50.0	50.0	56.9	57.8	0.0	0.0	1.305536	134182	136404	2355-2356	1.000000	70.0
ndhF_p5	ndhF	5	GACCCGTTTCTGAAGAGTCTT	AGGTCGGGTGAATTGGAAAC	21	20	47.6	50.0	57.6	58.1	4.7	0.0	1.482708	134182	136404	2353-2354	1.000000	68.5
ccsA_p1	ccsA	1	GGCGGCATAGCATCTCTTAA	TATGATTCGACAGGCTCTCG	20	20	50.0	50.0	57.8	56.9	3.1	5.8	1.237220	137741	138712	1170-1171	1.000000	70.3
ccsA_p2	ccsA	2	GGCGGCATAGCATCTCTTAA	ATATGATTCGACAGGCTCTCG	20	21	50.0	47.6	57.8	57.4	3.1	5.8	1.737409	137741	138712	1171-1172	1.000000	66.3
ccsA_p3	ccsA	3	TGGCGGCATAGCATCTCTTA	TATGATTCGACAGGCTCTCG	20	20	50.0	50.0	59.0	56.9	8.0	5.8	2.019587	137741	138712	1171-1172	1.000000	64.0
ccsA_p4	ccsA	4	GGCGGCATAGCATCTCTTAA	ATGATTCGACAGGCTCTCG	20	19	50.0	52.6	57.8	57.0	3.1	5.8	2.125123	137741	138712	1169-1170	1.000000	63.2
ccsA_p5	ccsA	5	GGCGGCATAGCATCTCTTAA	GATTCGACAGGCTCTCGC	20	18	50.0	61.1	57.8	58.0	3.1	7.3	2.174864	137741	138712	1167-1168	1.000000	62.8
psaC_p1	psaC	1	GCAAGGAAAATGGTTCGTGG	AGACCGAAAATTGATGGCGT	20	20	50.0	45.0	57.9	58.2	0.0	0.0	0.243357	140515	140760	417-422	1.000000	79.6
psaC_p2	psaC	2	TTTGCCCACTGTACATTGCT	AGACCGAAAATTGATGGCGT	20	20	45.0	45.0	58.0	58.2	7.7	0.0	0.184828	140515	140760	775-780	1.000000	79.4
psaC_p3	psaC	3	TTTGCCCACTGTACATTGCT	TGAAGCAGCTATTGGATCGG	20	20	45.0	50.0	58.0	57.8	7.7	0.0	0.251203	140515	140760	1108-1113	1.000000	78.7
psaC_p4	psaC	4	GAGAATCTCGAGTAACCGGC	AGACCGAAAATTGATGGCGT	20	20	55.0	45.0	57.9	58.2	13.1	0.0	0.301346	140515	140760	733-738	1.000000	78.6
psaC_p5	psaC	5	GCAAGGAAAATGGTTCGTGG	TGAAGCAGCTATTGGATCGG	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.309733	140515	140760	750-755	1.000000	78.5
ndhA_p1	ndhA	1	AGCATAGGGAACATATCGGA	ACTTGCAAATTCTTCCGCAG	20	20	45.0	45.0	55.5	57.3	0.0	0.0	3.228936	143076	145235	2362-2363	1.000000	54.3
ndhA_p2	ndhA	2	AGCATAGGGAACATATCGGA	CTTGCAAATTCTTCCGCAGT	20	20	45.0	45.0	55.5	57.3	0.0	0.0	3.228936	143076	145235	2361-2362	1.000000	54.3
ndhA_p3	ndhA	3	AGCATAGGGAACATATCGGAA	ACTTGCAAATTCTTCCGCAG	21	20	42.9	45.0	56.3	57.3	0.0	0.0	3.420868	143076	145235	2362-2363	1.000000	52.8
ndhA_p4	ndhA	4	AGCATAGGGAACATATCGGAA	CTTGCAAATTCTTCCGCAGT	21	20	42.9	45.0	56.3	57.3	0.0	0.0	3.420868	143076	145235	2361-2362	1.000000	52.8
ndhA_p5	ndhA	5	AGCATAGGGAACATATCGGA	TCAACTTGCAAATTCTTCCGC	20	21	45.0	42.9	55.5	58.0	0.0	0.0	3.528829	143076	145235	2365-2366	1.000000	51.9
ycf1_p1	ycf1	1	AGATTCGTAGATCCCGATTCT	ACGGACTCGATTGTGGAATG	21	20	42.9	50.0	55.8	58.0	17.4	0.0	3.242639	151021	151140	312-312	1.000000	54.6
ycf1_p2	ycf1	2	AGATTCGTAGATCCCGATTCT	CACGGACTCGATTGTGGAAT	21	20	42.9	50.0	55.8	58.0	17.4	0.0	3.242639	151021	151140	313-313	1.000000	54.6
ycf1_p3	ycf1	3	AGATTCGTAGATCCCGATTCT	CTCGAAACACGGACTCGATT	21	20	42.9	50.0	55.8	58.0	17.4	2.4	3.253952	151021	151140	320-320	1.000000	54.5
ycf1_p4	ycf1	4	AGATTCGTAGATCCCGATTCTT	ACGGACTCGATTGTGGAATG	22	20	40.9	50.0	56.5	58.0	17.4	0.0	3.490243	151021	151140	312-312	1.000000	52.6
ycf1_p5	ycf1	5	AGATTCGTAGATCCCGATTCTT	CACGGACTCGATTGTGGAAT	22	20	40.9	50.0	56.5	58.0	17.4	0.0	3.490243	151021	151140	313-313	1.000000	52.6