primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbB-clpP_p1	psbB-clpP	1	AACTAACGCGGTATGCATCA	TACGTCAATCCAAACGGCAT	20	20	45.0	45.0	57.7	57.9	12.5	0.0	0.405233	14335	15388	1114-1213	1.000000	83.6
psbB-clpP_p2	psbB-clpP	2	ACTAACGCGGTATGCATCAA	ATACGTCAATCCAAACGGCA	20	20	45.0	45.0	57.7	57.9	12.5	0.0	0.405233	14335	15388	1114-1213	1.000000	83.6
psbB-clpP_p3	psbB-clpP	3	ACTAACGCGGTATGCATCAA	TACGTCAATCCAAACGGCAT	20	20	45.0	45.0	57.7	57.9	12.5	0.0	0.405233	14335	15388	1113-1212	1.000000	83.6
psbB-clpP_p4	psbB-clpP	4	TAACGCGGTATGCATCAAGT	ATACGTCAATCCAAACGGCA	20	20	45.0	45.0	57.7	57.9	12.5	0.0	0.405233	14335	15388	1112-1211	1.000000	83.6
psbB-clpP_p5	psbB-clpP	5	TAACGCGGTATGCATCAAGT	TACGTCAATCCAAACGGCAT	20	20	45.0	45.0	57.7	57.9	12.5	0.0	0.405233	14335	15388	1111-1210	1.000000	83.6
rps4-trnS-GGA_p1	rps4-trnS-GGA	1	CACTACAGAGTGTGGGATTGG	CTCTTGCTTACCGAGGGTTC	21	20	52.4	55.0	58.0	58.0	0.0	0.0	1.033626	40832	41229	539-565	1.000000	75.6
rps4-trnS-GGA_p2	rps4-trnS-GGA	2	CACTACAGAGTGTGGGATTGG	GGGTTCGAATCCCTCTCTCT	21	20	52.4	55.0	58.0	58.2	0.0	17.2	1.239015	40832	41229	525-551	1.000000	74.1
rps4-trnS-GGA_p3	rps4-trnS-GGA	3	ACTACAGAGTGTGGGATTGGT	CTCTTGCTTACCGAGGGTTC	21	20	47.6	55.0	58.4	58.0	0.0	0.0	1.390470	40832	41229	538-564	1.000000	72.8
rps4-trnS-GGA_p4	rps4-trnS-GGA	4	ACTACAGAGTGTGGGATTGG	CTCTTGCTTACCGAGGGTTC	20	20	50.0	55.0	56.5	58.0	0.0	0.0	1.464394	40832	41229	538-564	1.000000	72.2
rps4-trnS-GGA_p5	rps4-trnS-GGA	5	CTACAGAGTGTGGGATTGGT	CTCTTGCTTACCGAGGGTTC	20	20	50.0	55.0	56.5	58.0	0.0	0.0	1.464394	40832	41229	537-563	1.000000	72.2
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	ACCCTTAGCCTTCCAAGCTA	CTGTCCTATCCATTAGACGATGG	20	23	50.0	47.8	58.0	57.9	0.0	0.0	3.098859	74570	75901	1404-1409	1.000000	63.7
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	ACCCTTAGCCTTCCAAGCTA	TGTCCTATCCATTAGACGATGG	20	22	50.0	45.5	58.0	56.9	0.0	0.0	3.171909	74570	75901	1403-1408	1.000000	63.1
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	TTCCAAGCTAATGATGCGGG	CTGTCCTATCCATTAGACGATGG	20	23	50.0	47.8	58.3	57.9	0.0	0.0	3.386565	74570	75901	1394-1399	1.000000	61.4
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	TTCCAAGCTAATGATGCGGG	TGTCCTATCCATTAGACGATGG	20	22	50.0	45.5	58.3	56.9	0.0	0.0	3.459615	74570	75901	1393-1398	1.000000	60.9
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	GCTAATGATGCGGGTTCGAT	CTGTCCTATCCATTAGACGATGG	20	23	50.0	47.8	58.5	57.9	0.0	0.0	3.552778	74570	75901	1388-1393	1.000000	60.2
atpH-atpI_p1	atpH-atpI	1	GCAGCAATAACAGAAGCAGC	TTTAGCTGCTCGGAATCCAC	20	20	50.0	50.0	58.1	58.0	0.0	4.9	0.118726	79526	80354	1442-1474	1.000000	84.5
atpH-atpI_p2	atpH-atpI	2	CGCAATACCTTCTACGGCTT	TTTAGCTGCTCGGAATCCAC	20	20	50.0	50.0	58.1	58.0	0.0	4.9	0.088243	79526	80354	1524-1556	1.000000	84.4
atpH-atpI_p3	atpH-atpI	3	TTCAGCTTCTGGTTGTCTCG	TTTAGCTGCTCGGAATCCAC	20	20	50.0	50.0	57.8	58.0	0.0	4.9	0.179029	79526	80354	1542-1574	1.000000	83.7
atpH-atpI_p4	atpH-atpI	4	TAGACCGAGTACCACACCTG	TTTAGCTGCTCGGAATCCAC	20	20	55.0	50.0	58.2	58.0	0.0	4.9	0.193897	79526	80354	2133-2145	1.000000	82.9
atpH-atpI_p5	atpH-atpI	5	CGCAATACCTTCTACGGCTT	TTTTGCAACTTTAGCCGCAG	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.196808	79526	80354	1028-1028	0.500000	58.4
trnL-CAA-ndhB_p1	trnL-CAA-ndhB	1	CTCGCGTGTCTACCATTTCA	ACCTATTTTGGTGCGGATGG	20	20	50.0	50.0	58.0	58.2	5.8	0.0	0.246493	96238	97193	1210-1273	1.000000	86.3
trnL-CAA-ndhB_p2	trnL-CAA-ndhB	2	CTCGCGTGTCTACCATTTCA	GCTTCTTGTTTTGCCTCGTG	20	20	50.0	50.0	58.0	58.2	5.8	0.0	0.250418	96238	97193	1283-1346	1.000000	86.2
trnL-CAA-ndhB_p3	trnL-CAA-ndhB	3	CTCGCGTGTCTACCATTTCA	ATTCAGTTCACGCACAGGAA	20	20	50.0	45.0	58.0	57.7	5.8	0.0	0.263747	96238	97193	1353-1416	1.000000	85.9
trnL-CAA-ndhB_p4	trnL-CAA-ndhB	4	CTCGCGTGTCTACCATTTCA	AGCTTCTTGTTTTGCCTCGT	20	20	50.0	45.0	58.0	58.3	5.8	0.0	0.328863	96238	97193	1284-1347	1.000000	85.6
trnL-CAA-ndhB_p5	trnL-CAA-ndhB	5	CTCGCGTGTCTACCATTTCA	TCTTGTTTTGCCTCGTGTCT	20	20	50.0	45.0	58.0	57.7	5.8	0.0	0.334784	96238	97193	1280-1343	1.000000	85.5
trnA-UGC-rrn23_p1	trnA-UGC-rrn23	1	TCCCGGCCTTCTTGGTATAA	GTCTCTGGGTGCCTAGGTAT	20	20	50.0	55.0	58.1	57.9	0.0	11.3	0.207292	107252	108267	1155-1165	1.000000	82.9
trnA-UGC-rrn23_p2	trnA-UGC-rrn23	2	TTCCCGGCCTTCTTGGTATA	GTCTCTGGGTGCCTAGGTAT	20	20	50.0	55.0	58.1	57.9	0.0	11.3	0.207292	107252	108267	1156-1166	1.000000	82.9
trnA-UGC-rrn23_p3	trnA-UGC-rrn23	3	TCCCGGCCTTCTTGGTATAA	GAATCTCCGGATCCATGCTC	20	20	50.0	55.0	58.1	57.9	0.0	13.4	0.209544	107252	108267	1221-1231	1.000000	82.6
trnA-UGC-rrn23_p4	trnA-UGC-rrn23	4	TTCCCGGCCTTCTTGGTATA	GAATCTCCGGATCCATGCTC	20	20	50.0	55.0	58.1	57.9	0.0	13.4	0.209544	107252	108267	1222-1232	1.000000	82.6
trnA-UGC-rrn23_p5	trnA-UGC-rrn23	5	TCCCGGCCTTCTTGGTATAA	TCATTTTCAGCTCCCCGAAG	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.231552	107252	108267	1203-1213	1.000000	82.5
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GTCACTTCGCAACGCTAAAC	TTCGAACCGAGATGCTCAAG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.070251	116799	117839	1697-1697	0.500000	59.4
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	ATGATGTTCGTCACTTCGCA	TTCGAACCGAGATGCTCAAG	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.082557	116799	117839	1706-1706	0.500000	59.3
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TGATGTTCGTCACTTCGCAA	TTCGAACCGAGATGCTCAAG	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.144325	116799	117839	1705-1705	0.500000	58.8
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GTCACTTCGCAACGCTAAAC	AAGCACTGCTTCCTAAGAGC	20	20	50.0	50.0	58.1	57.9	0.0	10.7	0.173020	116799	117839	1680-1680	0.500000	58.6
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	ATGATGTTCGTCACTTCGCA	AAGCACTGCTTCCTAAGAGC	20	20	45.0	50.0	57.9	57.9	0.0	10.7	0.185326	116799	117839	1689-1689	0.500000	58.5
ccsA_p1	ccsA	1	CTTGAGCATCTCGGTTCGAA	CTTCCTGGCAGGAACAAGTT	20	20	50.0	50.0	58.0	58.0	0.0	38.5	0.016826	118105	119136	1932-1932	0.500000	59.9
ccsA_p2	ccsA	2	CATCACCCCAAGATGGTACG	CTTCCTGGCAGGAACAAGTT	20	20	55.0	50.0	58.0	58.0	0.0	38.5	0.059559	118105	119136	2162-2162	0.500000	59.5
ccsA_p3	ccsA	3	CTTGAGCATCTCGGTTCGAA	TGCATTACCGGGTACAAGTG	20	20	50.0	50.0	58.0	57.9	0.0	6.3	0.105778	118105	119136	1812-1812	0.500000	59.2
ccsA_p4	ccsA	4	CTTGAGCATCTCGGTTCGAA	CCTTGCATTACCGGGTACAA	20	20	50.0	50.0	58.0	57.9	0.0	6.3	0.120535	118105	119136	1815-1815	0.500000	59.0
ccsA_p5	ccsA	5	CATCACCCCAAGATGGTACG	TGCATTACCGGGTACAAGTG	20	20	55.0	50.0	58.0	57.9	0.0	6.3	0.148511	118105	119136	2042-2042	0.500000	58.8
ndhD_p1	ndhD	1	AAAGGCTGGCAGGGTAAAAA	GCTTGTCCCACCGATGTATT	20	20	45.0	50.0	57.9	58.0	17.6	0.0	0.133991	119352	120824	2256-2256	0.500000	58.9
ndhD_p2	ndhD	2	TTGGTTACTTGGCTCGCATT	GCTTGTCCCACCGATGTATT	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.134100	119352	120824	2304-2304	0.500000	58.9
ndhD_p3	ndhD	3	TTTGGTTACTTGGCTCGCAT	GCTTGTCCCACCGATGTATT	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.134100	119352	120824	2305-2305	0.500000	58.9
ndhD_p4	ndhD	4	AAAGGCTGGCAGGGTAAAAA	TGGGTCTAGCCTACTAACCG	20	20	45.0	55.0	57.9	57.9	17.6	0.0	0.158575	119352	120824	2084-2084	0.500000	58.7
ndhD_p5	ndhD	5	TTGGTTACTTGGCTCGCATT	TGGGTCTAGCCTACTAACCG	20	20	45.0	55.0	58.1	57.9	0.0	0.0	0.158684	119352	120824	2132-2132	0.500000	58.7
ndhG_p1	ndhG	1	GGCTGATCCTATTGCTGCTT	GCGGAAACTGTGTCGAGTAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.029376	122025	122585	1346-1346	0.500000	59.8
ndhG_p2	ndhG	2	GGCTGATCCTATTGCTGCTT	GCCCTTATCAGTGGTGGAAG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.065668	122025	122585	1231-1231	0.500000	59.5
ndhG_p3	ndhG	3	ATGGCTGATCCTATTGCTGC	GCGGAAACTGTGTCGAGTAT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.100841	122025	122585	1348-1348	0.500000	59.2
ndhG_p4	ndhG	4	GGCTGATCCTATTGCTGCTT	AACTGTGTCGAGTATTGCCC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.116308	122025	122585	1341-1341	0.500000	59.1
ndhG_p5	ndhG	5	GGCTGATCCTATTGCTGCTT	AGCAAACTATACAAGCCGCA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.129496	122025	122585	1628-1628	0.500000	59.0
ndhA_p1	ndhA	1	ATACTCGACACAGTTTCCGC	AAATTTAGAGGCCCGACGAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.027778	123450	125380	2687-2687	0.500000	59.8
ndhA_p2	ndhA	2	ATACTCGACACAGTTTCCGC	GTCCTATGCTACGAGCTTCG	20	20	50.0	55.0	58.0	58.0	0.0	8.1	0.047157	123450	125380	2889-2889	0.500000	59.6
ndhA_p3	ndhA	3	CTTCCACCACTGATAAGGGC	AAATTTAGAGGCCCGACGAC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.064070	123450	125380	2802-2802	0.500000	59.5
ndhA_p4	ndhA	4	ATACTCGACACAGTTTCCGC	ATTTAGAGGCCCGACGACTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.070801	123450	125380	2685-2685	0.500000	59.4
ndhA_p5	ndhA	5	ATACTCGACACAGTTTCCGC	TAGAGGCCCGACGACTAAAT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.070801	123450	125380	2682-2682	0.500000	59.4
ndhH_p1	ndhH	1	GGCTCGAAGACCACCTAAAG	TCCTCCTCGGTTTCTGAAGA	20	20	55.0	50.0	58.0	57.7	0.0	0.0	0.315762	125382	126563	2028-2028	0.500000	57.5
ndhH_p2	ndhH	2	ACGAGTCGCACGTAGAGATA	TCCTCCTCGGTTTCTGAAGA	20	20	50.0	50.0	58.1	57.7	13.6	0.0	0.372315	125382	126563	2100-2100	0.500000	57.0
ndhH_p3	ndhH	3	CGTGTTCAGGTCCAATACGT	TCCTCCTCGGTTTCTGAAGA	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.378768	125382	126563	1735-1735	0.500000	57.0
ndhH_p4	ndhH	4	CAGCGTGTTCAGGTCCAATA	TCCTCCTCGGTTTCTGAAGA	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.397528	125382	126563	1738-1738	0.500000	56.8
ndhH_p5	ndhH	5	AGCGTGTTCAGGTCCAATAC	TCCTCCTCGGTTTCTGAAGA	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.398984	125382	126563	1737-1737	0.500000	56.8
rps15-ycf1_p1	rps15-ycf1	1	TGTAGCAAGCACGGTCATAC	TGGGAGTCGATTTGCTATGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.051024	126827	127365	875-875	0.500000	59.6
rps15-ycf1_p2	rps15-ycf1	2	AACAGGTGAGAAGCTATGCG	TGGGAGTCGATTTGCTATGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.062177	126827	127365	1204-1204	0.500000	59.5
rps15-ycf1_p3	rps15-ycf1	3	TGTAGCAAGCACGGTCATAC	ACCGCGAGTATTTGATGACC	20	20	50.0	50.0	58.0	58.1	0.0	8.2	0.068650	126827	127365	1292-1292	0.500000	59.5
rps15-ycf1_p4	rps15-ycf1	4	TGTAGCAAGCACGGTCATAC	CTACTTCCAAAGCGCCGTAT	20	20	50.0	50.0	58.0	58.1	0.0	2.3	0.068650	126827	127365	1111-1111	0.500000	59.5
rps15-ycf1_p5	rps15-ycf1	5	TGTAGCAAGCACGGTCATAC	CCGCGAGTATTTGATGACCA	20	20	50.0	50.0	58.0	58.1	0.0	8.2	0.069994	126827	127365	1291-1291	0.500000	59.4
ycf1_p1	ycf1	1	GGAAAACCCCTACCCCAATG	GCAATATCGGAGTCACCCTG	20	20	55.0	55.0	58.1	58.1	0.0	0.0	0.268279	130327	130446	1091-1091	0.500000	57.9
ycf1_p2	ycf1	2	GGAAAACCCCTACCCCAATG	CGGAGTCACCCTGGATTTTT	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.339732	130327	130446	1084-1084	0.500000	57.3
ycf1_p3	ycf1	3	TGAGCTGATGCAAAATCCCA	GCAATATCGGAGTCACCCTG	20	20	45.0	55.0	57.8	58.1	0.0	0.0	0.348000	130327	130446	1073-1073	0.500000	57.2
ycf1_p4	ycf1	4	CCTACCCCAATGAGCTGATG	GCAATATCGGAGTCACCCTG	20	20	55.0	55.0	57.7	58.1	0.0	0.0	0.396094	130327	130446	1083-1083	0.500000	56.8
ycf1_p5	ycf1	5	TGAGCTGATGCAAAATCCCA	CGGAGTCACCCTGGATTTTT	20	20	45.0	50.0	57.8	57.8	0.0	0.0	0.419453	130327	130446	1066-1066	0.500000	56.6