primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TAGTTGACAGTCAGGGTCGT	20	20	55.0	50.0	58.1	58.0	0.0	2.8	0.137799	78	791	881-930	1.000000	87.1
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	78	791	780-829	1.000000	87.1
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	TAGTTGACAGTCAGGGTCGT	20	20	50.0	50.0	57.9	58.0	0.0	2.8	0.142160	78	791	895-944	1.000000	87.0
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	TAGTTGACAGTCAGGGTCGT	20	20	50.0	50.0	57.9	58.0	0.0	2.8	0.143668	78	791	894-943	1.000000	86.9
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	TAGTTGACAGTCAGGGTCGT	20	20	50.0	50.0	57.9	58.0	0.0	2.8	0.143668	78	791	896-945	1.000000	86.9
psbC-trnS-UGA_p1	psbC-trnS-UGA	1	CCTGTTCTTTCCATGACCCC	GGGTTCGAATCCCTCTCTCT	20	20	55.0	55.0	58.2	58.2	0.0	17.2	0.377505	41546	41668	175-176	1.000000	82.9
psbC-trnS-UGA_p2	psbC-trnS-UGA	2	CCTGTTCTTTCCATGACCCC	GGTTGATAGCTCCGGTCTTG	20	20	55.0	55.0	58.2	58.1	0.0	0.0	0.219251	41546	41668	229-230	1.000000	82.8
psbC-trnS-UGA_p3	psbC-trnS-UGA	3	TTCCATGACCCCTCTTAGCT	GGTTGATAGCTCCGGTCTTG	20	20	50.0	55.0	57.7	58.1	0.0	0.0	0.329109	41546	41668	221-222	1.000000	82.1
psbC-trnS-UGA_p4	psbC-trnS-UGA	4	CCTGTTCTTTCCATGACCCC	GATGGCCGAGTGGTTGATAG	20	20	55.0	55.0	58.2	58.1	0.0	0.0	0.289496	41546	41668	240-241	1.000000	82.0
psbC-trnS-UGA_p5	psbC-trnS-UGA	5	TTGTGGGTCATTTGTGGCAT	GGTTGATAGCTCCGGTCTTG	20	20	45.0	55.0	58.3	58.1	0.0	0.0	0.344134	41546	41668	309-310	1.000000	80.6
petD-rpoA_p1	petD-rpoA	1	TTTTTGATCGGTACCGCAGT	AGACATTCTACAGAAACATTTCACA	20	25	45.0	32.0	57.8	57.0	29.6	7.1	6.215001	87356	87546	333-336	1.000000	44.5
petD-rpoA_p2	petD-rpoA	2	CGTCGTCCAGTAGCTACAAC	AGACATTCTACAGAAACATTTCACA	20	25	55.0	32.0	58.1	57.0	25.3	7.1	6.149683	87356	87546	357-360	1.000000	44.2
petD-rpoA_p3	petD-rpoA	3	TCGTCGTCCAGTAGCTACAA	AGACATTCTACAGAAACATTTCACA	20	25	50.0	32.0	58.2	57.0	25.3	7.1	6.229915	87356	87546	358-361	1.000000	44.2
petD-rpoA_p4	petD-rpoA	4	TTCGTCGTCCAGTAGCTACA	AGACATTCTACAGAAACATTTCACA	20	25	50.0	32.0	58.2	57.0	25.3	7.1	6.229915	87356	87546	359-362	1.000000	44.2
petD-rpoA_p5	petD-rpoA	5	CATTTCGTCGTCCAGTAGCT	AGACATTCTACAGAAACATTTCACA	20	25	50.0	32.0	57.7	57.0	0.0	7.1	6.321733	87356	87546	362-365	1.000000	44.2
rpl22-rps19_p1	rpl22-rps19	1	GTACGCGGTTTACTCGAGTT	GACATGCGCGAAATGACAAT	20	20	50.0	45.0	58.0	57.8	25.4	23.8	0.209371	93983	94080	174-182	1.000000	84.2
rpl22-rps19_p2	rpl22-rps19	2	TTCTGTACGCGGTTTACTCG	GACATGCGCGAAATGACAAT	20	20	50.0	45.0	58.0	57.8	12.5	23.8	0.210712	93983	94080	178-186	1.000000	84.0
rpl22-rps19_p3	rpl22-rps19	3	GTACGCGGTTTACTCGAGTT	GGAGAATTCGCACCTACTCG	20	20	50.0	55.0	58.0	58.2	25.4	11.5	0.189447	93983	94080	205-213	1.000000	83.5
rpl22-rps19_p4	rpl22-rps19	4	TTCTGTACGCGGTTTACTCG	GGAGAATTCGCACCTACTCG	20	20	50.0	55.0	58.0	58.2	12.5	11.5	0.190789	93983	94080	209-217	1.000000	83.4
rpl22-rps19_p5	rpl22-rps19	5	GTACGCGGTTTACTCGAGTT	GGGAGAATTCGCACCTACTC	20	20	50.0	55.0	58.0	57.8	25.4	11.5	0.257499	93983	94080	206-214	1.000000	82.9
rrn4.5-rrn5_p1	rrn4.5-rrn5	1	CAGCTGAGGCATCCTAACAG	AGTATCGTCACCGCAGTAGA	20	20	55.0	50.0	58.0	58.0	8.4	0.0	0.083276	118219	118442	341-349	1.000000	81.9
rrn4.5-rrn5_p2	rrn4.5-rrn5	2	CAGCTGAGGCATCCTAACAG	TATCGTCACCGCAGTAGAGT	20	20	55.0	50.0	58.0	58.0	8.4	0.0	0.083276	118219	118442	339-347	1.000000	81.9
rrn4.5-rrn5_p3	rrn4.5-rrn5	3	GCTGAGGCATCCTAACAGAC	TATCGTCACCGCAGTAGAGT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.087359	118219	118442	337-345	1.000000	81.9
rrn4.5-rrn5_p4	rrn4.5-rrn5	4	GTCAAGTGGAAGTGCAGTGA	AGTATCGTCACCGCAGTAGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.080545	118219	118442	367-375	1.000000	81.7
rrn4.5-rrn5_p5	rrn4.5-rrn5	5	GTCAAGTGGAAGTGCAGTGA	TATCGTCACCGCAGTAGAGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.080545	118219	118442	365-373	1.000000	81.7
ycf1_p1	ycf1	1	CCGAGTGGTACGAGGATTTG	AATAGTCCTCGTGTCCCACA	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.144408	123202	123321	1231-1231	0.500000	58.8
ycf1_p2	ycf1	2	CCGAGTGGTACGAGGATTTG	ATAGTCCTCGTGTCCCACAA	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.144408	123202	123321	1230-1230	0.500000	58.8
ycf1_p3	ycf1	3	GAACTCCCTTTCGGTTCTCC	AATAGTCCTCGTGTCCCACA	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.163199	123202	123321	971-971	0.500000	58.7
ycf1_p4	ycf1	4	GAACTCCCTTTCGGTTCTCC	ATAGTCCTCGTGTCCCACAA	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.163199	123202	123321	970-970	0.500000	58.7
ycf1_p5	ycf1	5	GGAACTCCCTTTCGGTTCTC	ATAGTCCTCGTGTCCCACAA	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.163199	123202	123321	971-971	0.500000	58.7
ndhH_p1	ndhH	1	GTGTGTAGTCTGTGAGCGAG	GCTTGGTACCATCCGCTAAA	20	20	55.0	50.0	58.0	58.0	0.0	17.2	0.062234	126805	127986	2163-2163	0.500000	59.5
ndhH_p2	ndhH	2	GTGTGTAGTCTGTGAGCGAG	TTGGTACCATCCGCTAAAGC	20	20	55.0	50.0	58.0	58.0	0.0	17.2	0.062234	126805	127986	2161-2161	0.500000	59.5
ndhH_p3	ndhH	3	GTGTGTAGTCTGTGAGCGAG	TAGCTTGGTACCATCCGCTA	20	20	55.0	50.0	58.0	57.9	0.0	25.3	0.105760	126805	127986	2165-2165	0.500000	59.2
ndhH_p4	ndhH	4	GTGTGTAGTCTGTGAGCGAG	TCAGGTCCAATACGTTGCTG	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.122860	126805	127986	2113-2113	0.500000	59.0
ndhH_p5	ndhH	5	GTGTGTAGTCTGTGAGCGAG	TGACTGCTATTGTTGGTCCG	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.122860	126805	127986	2238-2238	0.500000	59.0
ndhA_p1	ndhA	1	CAATGTTACGAGCTTCCGGA	CAATATACCTTGCAGCCCGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.037264	127988	130179	2877-2877	0.500000	59.7
ndhA_p2	ndhA	2	CAATGTTACGAGCTTCCGGA	CCCGTACTGTTTGTTGACCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048286	127988	130179	2862-2862	0.500000	59.6
ndhA_p3	ndhA	3	TCGGTGAAATGACGGAATCC	CAATATACCTTGCAGCCCGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.051223	127988	130179	2748-2748	0.500000	59.6
ndhA_p4	ndhA	4	TCGGTGAAATGACGGAATCC	CCCGTACTGTTTGTTGACCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.062245	127988	130179	2733-2733	0.500000	59.5
ndhA_p5	ndhA	5	GGCTTATCGGGACCAATGTT	CAATATACCTTGCAGCCCGT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.085464	127988	130179	2890-2890	0.500000	59.3
ndhI_p1	ndhI	1	CTGGAATAGCGACGGAAACA	TGTAGTGGATTAGTGTAGATATATT	20	25	50.0	28.0	58.0	52.6	0.0	0.0	10.381822	130279	130821	1974-1974	0.500000	20.0
ndhI_p2	ndhI	2	TGGAATAGCGACGGAAACAG	TGTAGTGGATTAGTGTAGATATATT	20	25	50.0	28.0	58.0	52.6	0.0	0.0	10.381822	130279	130821	1973-1973	0.500000	20.0
ndhI_p3	ndhI	3	GGAATAGCGACGGAAACAGT	TGTAGTGGATTAGTGTAGATATATT	20	25	50.0	28.0	58.0	52.6	0.0	0.0	10.383210	130279	130821	1972-1972	0.500000	20.0
ndhI_p4	ndhI	4	TTTTGGGGTGGAATCTGTGG	TGTAGTGGATTAGTGTAGATATATT	20	25	50.0	28.0	58.0	52.6	0.0	0.0	10.385482	130279	130821	1877-1877	0.500000	20.0
ndhI_p5	ndhI	5	TAGCGACGGAAACAGTGATG	TGTAGTGGATTAGTGTAGATATATT	20	25	50.0	28.0	58.0	52.6	0.0	0.0	10.386440	130279	130821	1968-1968	0.500000	20.0
ndhI-ndhG_p1	ndhI-ndhG	1	ACGGGCTGCAAGGTATATTG	ACAAGAACCGATCCCAGAGA	20	20	50.0	50.0	58.0	58.1	0.5	0.0	0.094097	130822	131725	1531-1531	0.500000	59.2
ndhI-ndhG_p2	ndhI-ndhG	2	TGGTCAACAAACAGTACGGG	ACAAGAACCGATCCCAGAGA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.105119	130822	131725	1546-1546	0.500000	59.2
ndhI-ndhG_p3	ndhI-ndhG	3	ACGGGCTGCAAGGTATATTG	CCCTCATGAACATCACAGCA	20	20	50.0	50.0	58.0	57.9	0.5	0.0	0.150995	130822	131725	1647-1647	0.500000	58.8
ndhI-ndhG_p4	ndhI-ndhG	4	TGGTCAACAAACAGTACGGG	CCCTCATGAACATCACAGCA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.162016	130822	131725	1662-1662	0.500000	58.7
ndhI-ndhG_p5	ndhI-ndhG	5	ACGGGCTGCAAGGTATATTG	ACAAACTAGCGAAGTGACCC	20	20	50.0	50.0	58.0	57.8	0.5	0.0	0.198000	130822	131725	1712-1712	0.500000	58.4
ndhE-psaC_p1	ndhE-psaC	1	GTCGAAGCATGGTTAGAGCA	GAAGAGCCCGCGAATATAGG	20	20	50.0	55.0	58.0	58.0	18.9	8.2	0.012476	132970	133588	1294-1294	0.500000	59.9
ndhE-psaC_p2	ndhE-psaC	2	GTCGAAGCATGGTTAGAGCA	GGAAGAGCCCGCGAATATAG	20	20	50.0	55.0	58.0	58.0	18.9	8.2	0.012476	132970	133588	1295-1295	0.500000	59.9
ndhE-psaC_p3	ndhE-psaC	3	TCGAAGCATGGTTAGAGCAC	GAAGAGCCCGCGAATATAGG	20	20	50.0	55.0	58.0	58.0	15.3	8.2	0.012476	132970	133588	1293-1293	0.500000	59.9
ndhE-psaC_p4	ndhE-psaC	4	TCGAAGCATGGTTAGAGCAC	GGAAGAGCCCGCGAATATAG	20	20	50.0	55.0	58.0	58.0	15.3	8.2	0.012476	132970	133588	1294-1294	0.500000	59.9
ndhE-psaC_p5	ndhE-psaC	5	TCGAAGCATGGTTAGAGCAC	TACTGCGAGTTGTCTCATGC	20	20	50.0	50.0	58.0	58.0	15.3	0.0	0.013853	132970	133588	1083-1083	0.500000	59.9
ndhD_p1	ndhD	1	GCATGAGACAACTCGCAGTA	GGGCTCCTATTGGAATTGGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.004784	133969	135471	2104-2104	0.500000	60.0
ndhD_p2	ndhD	2	GCATGAGACAACTCGCAGTA	GGGGCTCCTATTGGAATTGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.004784	133969	135471	2105-2105	0.500000	60.0
ndhD_p3	ndhD	3	TATGGCATGAGACAACTCGC	GGGCTCCTATTGGAATTGGG	20	20	50.0	55.0	58.1	58.0	11.0	0.0	0.061102	133969	135471	2108-2108	0.500000	59.5
ndhD_p4	ndhD	4	TATGGCATGAGACAACTCGC	GGGGCTCCTATTGGAATTGG	20	20	50.0	55.0	58.1	58.0	11.0	0.0	0.061102	133969	135471	2109-2109	0.500000	59.5
ndhD_p5	ndhD	5	GCATGAGACAACTCGCAGTA	CCCTAAAGAAACTTGGGCGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.098071	133969	135471	2083-2083	0.500000	59.2
ccsA_p1	ccsA	1	TTGCATTGCCAGGAATGAGT	GTGAAATCGGTAGACACGCT	20	20	45.0	50.0	58.1	58.0	15.1	0.0	0.080343	135685	136656	1688-1688	0.500000	59.4
ccsA_p2	ccsA	2	TTGCATTGCCAGGAATGAGT	TGAAATCGGTAGACACGCTG	20	20	45.0	50.0	58.1	58.0	15.1	0.0	0.081713	135685	136656	1687-1687	0.500000	59.3
ccsA_p3	ccsA	3	TGACGAAATGGGAGGGGTAT	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.120033	135685	136656	1762-1762	0.500000	59.0
ccsA_p4	ccsA	4	TGACGAAATGGGAGGGGTAT	TGAAATCGGTAGACACGCTG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.121404	135685	136656	1761-1761	0.500000	59.0
ccsA_p5	ccsA	5	CATTGCCAGGAATGAGTGGT	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	58.2	58.0	6.0	0.0	0.164462	135685	136656	1685-1685	0.500000	58.7
rpl32-ndhF_p1	rpl32-ndhF	1	GTTGCGGTAAGGGTAAGTCA	CTGCAATTGGTCGTGTGAAC	20	20	50.0	50.0	57.5	58.0	0.0	7.7	0.497314	137668	138945	2302-2302	0.500000	56.0
rpl32-ndhF_p2	rpl32-ndhF	2	GTTGCGGTAAGGGTAAGTCA	GGGAGTGGAAGGAAGGGTAT	20	20	50.0	55.0	57.5	58.1	0.0	0.0	0.566058	137668	138945	1838-1838	0.500000	55.5
rpl32-ndhF_p3	rpl32-ndhF	3	GTTGCGGTAAGGGTAAGTCA	AGGAAAAGCCCACATACGAC	20	20	50.0	50.0	57.5	57.9	0.0	0.0	0.570182	137668	138945	1917-1917	0.500000	55.4
rpl32-ndhF_p4	rpl32-ndhF	4	GTTGCGGTAAGGGTAAGTCA	GAGGCACACAGAGTAGCAAA	20	20	50.0	50.0	57.5	57.8	0.0	0.0	0.630590	137668	138945	2480-2480	0.500000	55.0
rpl32-ndhF_p5	rpl32-ndhF	5	GTTGCGGTAAGGGTAAGTCA	AAAAGCCCACATACGACGAA	20	20	50.0	45.0	57.5	57.8	0.0	0.0	0.633608	137668	138945	1914-1914	0.500000	54.9
ndhF_p1	ndhF	1	AGAGTTGGTGAATCGGAAACA	TCTCTTATCTTACCGTCATACT	21	22	42.9	36.4	57.5	53.3	0.0	0.0	8.182155	138946	141174	2549-2549	0.500000	20.0
ndhF_p2	ndhF	2	AGAGTTGGTGAATCGGAAACA	TCTCTTATCTTACCGTCATACTT	21	23	42.9	34.8	57.5	54.1	0.0	0.0	8.350710	138946	141174	2549-2549	0.500000	20.0
ndhF_p3	ndhF	3	AGAGTTGGTGAATCGGAAACA	TTCTCTTATCTTACCGTCATACT	21	23	42.9	34.8	57.5	54.1	0.0	0.0	8.350710	138946	141174	2550-2550	0.500000	20.0
ndhF_p4	ndhF	4	AGAGTTGGTGAATCGGAAACA	TTCTCTTATCTTACCGTCATACTT	21	24	42.9	33.3	57.5	54.9	0.0	0.0	8.588831	138946	141174	2550-2550	0.500000	20.0
ndhF_p5	ndhF	5	AGAGTTGGTGAATCGGAAACA	TTTCTCTTATCTTACCGTCATACT	21	24	42.9	33.3	57.5	54.9	0.0	0.0	8.588831	138946	141174	2551-2551	0.500000	20.0
ndhF-trnN_p1	ndhF-trnN	1	ACAAACAGCGTTGGGGTTAT	CCAGGTATTGACTGACGGTG	20	20	45.0	55.0	58.0	58.0	3.7	0.0	0.020439	141175	143460	2662-2662	0.500000	59.8
ndhF-trnN_p2	ndhF-trnN	2	ATTACAAACAGCGTTGGGGT	CCAGGTATTGACTGACGGTG	20	20	45.0	55.0	58.0	58.0	3.7	0.0	0.020439	141175	143460	2665-2665	0.500000	59.8
ndhF-trnN_p3	ndhF-trnN	3	ACAAACAGCGTTGGGGTTAT	CGGGAGAATTGATGACTGCA	20	20	45.0	50.0	58.0	58.0	3.7	0.0	0.038714	141175	143460	2778-2778	0.500000	59.7
ndhF-trnN_p4	ndhF-trnN	4	ATTACAAACAGCGTTGGGGT	CGGGAGAATTGATGACTGCA	20	20	45.0	50.0	58.0	58.0	3.7	0.0	0.038714	141175	143460	2781-2781	0.500000	59.7
ndhF-trnN_p5	ndhF-trnN	5	TTTCTTTGCGGGTGAAGGAA	CCAGGTATTGACTGACGGTG	20	20	45.0	55.0	57.9	58.0	0.0	0.0	0.070195	141175	143460	2639-2639	0.500000	59.4
rrn5-rrn4.5_p1	rrn5-rrn4.5	1	AGTATCGTCACCGCAGTAGA	CAGCTGAGGCATCCTAACAG	20	20	50.0	55.0	58.0	58.0	0.0	8.4	0.083276	144561	144784	341-349	1.000000	81.9
rrn5-rrn4.5_p2	rrn5-rrn4.5	2	TATCGTCACCGCAGTAGAGT	CAGCTGAGGCATCCTAACAG	20	20	50.0	55.0	58.0	58.0	0.0	8.4	0.083276	144561	144784	339-347	1.000000	81.9
rrn5-rrn4.5_p3	rrn5-rrn4.5	3	TATCGTCACCGCAGTAGAGT	GCTGAGGCATCCTAACAGAC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.087359	144561	144784	337-345	1.000000	81.9
rrn5-rrn4.5_p4	rrn5-rrn4.5	4	AGTATCGTCACCGCAGTAGA	GTCAAGTGGAAGTGCAGTGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.080545	144561	144784	367-375	1.000000	81.7
rrn5-rrn4.5_p5	rrn5-rrn4.5	5	TATCGTCACCGCAGTAGAGT	GTCAAGTGGAAGTGCAGTGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.080545	144561	144784	365-373	1.000000	81.7