primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ(UUG)_p1	rps16-trnQ(UUG)	1	CGGATCTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.1	58.1	0.0	16.9	0.161467	6441	8101	1743-1748	1.000000	79.4
rps16-trnQ(UUG)_p2	rps16-trnQ(UUG)	2	ATCTGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	0.0	16.9	0.162155	6441	8101	1740-1745	1.000000	79.4
rps16-trnQ(UUG)_p3	rps16-trnQ(UUG)	3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6441	8101	1729-1734	1.000000	79.0
rps16-trnQ(UUG)_p4	rps16-trnQ(UUG)	4	CCACAACGGATCTGTCCTTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.3	58.1	7.9	16.9	0.349248	6441	8101	1749-1754	1.000000	77.9
rps16-trnQ(UUG)_p5	rps16-trnQ(UUG)	5	GGATCTGTCCTTCAAGTCGC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	0.0	16.9	0.429912	6441	8101	1742-1747	1.000000	77.3
trnS(GCU)-trnG(GCC)_p1	trnS(GCU)-trnG(GCC)	1	CAATCCGACGCTTTAGTCCA	AGACCGAAAGACCCTTTAACT	20	21	50.0	42.9	58.0	56.6	0.0	0.0	2.425660	9417	10109	799-800	1.000000	61.6
trnS(GCU)-trnG(GCC)_p2	trnS(GCU)-trnG(GCC)	2	AATCCGACGCTTTAGTCCAC	AGACCGAAAGACCCTTTAACT	20	21	50.0	42.9	58.0	56.6	0.0	0.0	2.427048	9417	10109	798-799	1.000000	61.6
trnS(GCU)-trnG(GCC)_p3	trnS(GCU)-trnG(GCC)	3	ATCAACGGAACCGGAAAGAG	AGACCGAAAGACCCTTTAACT	20	21	50.0	42.9	57.9	56.6	9.5	0.0	2.515840	9417	10109	863-864	1.000000	60.8
trnS(GCU)-trnG(GCC)_p4	trnS(GCU)-trnG(GCC)	4	CATCAACGGAACCGGAAAGA	AGACCGAAAGACCCTTTAACT	20	21	50.0	42.9	58.2	56.6	9.5	0.0	2.617743	9417	10109	864-865	1.000000	60.0
trnS(GCU)-trnG(GCC)_p5	trnS(GCU)-trnG(GCC)	5	TCATCAACGGAACCGGAAAG	AGACCGAAAGACCCTTTAACT	20	21	50.0	42.9	58.2	56.6	9.5	0.0	2.617743	9417	10109	865-866	1.000000	60.0
trnG(GCC)-trnR(UCU)_p1	trnG(GCC)-trnR(UCU)	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	10858	11021	247-247	1.000000	64.3
trnG(GCC)-trnR(UCU)_p2	trnG(GCC)-trnR(UCU)	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	10858	11021	250-250	1.000000	64.1
trnG(GCC)-trnR(UCU)_p3	trnG(GCC)-trnR(UCU)	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	10858	11021	253-253	1.000000	63.6
trnG(GCC)-trnR(UCU)_p4	trnG(GCC)-trnR(UCU)	4	AGCCTTCCAAGCTAACGATG	CATTAGACAATGGACGCTGT	20	20	50.0	45.0	58.0	55.9	0.0	0.0	2.177871	10858	11021	228-228	1.000000	63.6
trnG(GCC)-trnR(UCU)_p5	trnG(GCC)-trnR(UCU)	5	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	10858	11021	256-256	1.000000	63.4
atpH-atpI_p1	atpH-atpI	1	AACAGAAGCGGCAGAAATCA	TCCCTGTCATGTTCCTTGGA	20	20	45.0	50.0	58.1	58.3	1.4	0.0	0.362752	14655	15723	1203-1203	1.000000	77.3
atpH-atpI_p2	atpH-atpI	2	TACCCTCTACAGCTTGACCC	TCCCTGTCATGTTCCTTGGA	20	20	55.0	50.0	58.1	58.3	0.0	0.0	0.396990	14655	15723	1288-1288	1.000000	77.0
atpH-atpI_p3	atpH-atpI	3	GCGATACCCTCTACAGCTTG	TCCCTGTCATGTTCCTTGGA	20	20	55.0	50.0	57.9	58.3	0.0	0.0	0.400507	14655	15723	1292-1292	1.000000	77.0
atpH-atpI_p4	atpH-atpI	4	CAGCAGCAATAACAGAAGCG	TCCCTGTCATGTTCCTTGGA	20	20	50.0	50.0	57.8	58.3	0.0	0.0	0.428635	14655	15723	1213-1213	1.000000	76.8
atpH-atpI_p5	atpH-atpI	5	GCCAATCCAGCAGCAATAAC	TCCCTGTCATGTTCCTTGGA	20	20	50.0	50.0	57.8	58.3	0.0	0.0	0.473359	14655	15723	1220-1220	1.000000	76.4
rps2-rpoC2_p1	rps2-rpoC2	1	AACTCCTGCCTCCATCATCT	TTCGAGGGGGAAATGAGAGA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.357147	17400	17606	377-382	1.000000	78.2
rps2-rpoC2_p2	rps2-rpoC2	2	TGCCTCCATCATCTCTTCCA	TTCGAGGGGGAAATGAGAGA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.364021	17400	17606	371-376	1.000000	78.2
rps2-rpoC2_p3	rps2-rpoC2	3	TCCTGCCTCCATCATCTCTT	TTCGAGGGGGAAATGAGAGA	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.434938	17400	17606	374-379	1.000000	77.6
rps2-rpoC2_p4	rps2-rpoC2	4	GAACTCCTGCCTCCATCATC	TTCGAGGGGGAAATGAGAGA	20	20	55.0	50.0	57.7	57.8	0.0	0.0	0.500676	17400	17606	378-383	1.000000	77.0
rps2-rpoC2_p5	rps2-rpoC2	5	CTCCTGCCTCCATCATCTCT	TTCGAGGGGGAAATGAGAGA	20	20	55.0	50.0	58.3	57.8	0.0	0.0	0.528170	17400	17606	375-380	1.000000	76.8
rpoB-trnC(GCA)_p1	rpoB-trnC(GCA)	1	CTCATTTCCATCCCCGAGC	CCGGATTTGAACTGGGGAAA	19	20	57.9	50.0	58.0	58.1	0.0	0.0	1.094688	27988	29168	1246-1264	1.000000	72.6
rpoB-trnC(GCA)_p2	rpoB-trnC(GCA)	2	ATTCCCTCATTTCCATCCCC	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	56.9	58.1	0.0	0.0	1.163184	27988	29168	1251-1269	1.000000	72.1
rpoB-trnC(GCA)_p3	rpoB-trnC(GCA)	3	CTCATTTCCATCCCCGAGC	AAAGGATTTGCAGTCCTCCG	19	20	57.9	50.0	58.0	58.2	0.0	19.5	1.186175	27988	29168	1227-1245	1.000000	71.9
rpoB-trnC(GCA)_p4	rpoB-trnC(GCA)	4	TCATTTCCATCCCCGAGCAT	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	59.2	58.1	0.0	0.0	1.236113	27988	29168	1245-1263	1.000000	71.5
rpoB-trnC(GCA)_p5	rpoB-trnC(GCA)	5	ATTCCCTCATTTCCATCCCC	AAAGGATTTGCAGTCCTCCG	20	20	50.0	50.0	56.9	58.2	0.0	19.5	1.254671	27988	29168	1232-1250	1.000000	71.4
trnM(CAU)-psbD_p1	trnM(CAU)-psbD	1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	20	20	55.0	50.0	58.1	58.1	7.6	0.0	0.177181	33272	34589	1475-1480	1.000000	79.1
trnM(CAU)-psbD_p2	trnM(CAU)-psbD	2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.199336	33272	34589	1478-1483	1.000000	78.9
trnM(CAU)-psbD_p3	trnM(CAU)-psbD	3	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	33272	34589	1465-1470	1.000000	78.0
trnM(CAU)-psbD_p4	trnM(CAU)-psbD	4	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.332292	33272	34589	1468-1473	1.000000	77.8
trnM(CAU)-psbD_p5	trnM(CAU)-psbD	5	GGCGTAAGTCATCGGTTCAA	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.395920	33272	34589	1452-1457	1.000000	77.3
psbZ-trnG(GCC)_p1	psbZ-trnG(GCC)	1	TTGCTTCTCCTGATGGTTGG	AATCGAACCCGCATCTTCTC	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.263697	37879	38170	459-460	1.000000	79.1
psbZ-trnG(GCC)_p2	psbZ-trnG(GCC)	2	TTGCTTCTCCTGATGGTTGG	GAATCGAACCCGCATCTTCT	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.263697	37879	38170	460-461	1.000000	79.1
psbZ-trnG(GCC)_p3	psbZ-trnG(GCC)	3	GCTTCTCCTGATGGTTGGTC	AATCGAACCCGCATCTTCTC	20	20	55.0	50.0	58.3	58.1	0.0	0.0	0.314679	37879	38170	457-458	1.000000	78.7
psbZ-trnG(GCC)_p4	psbZ-trnG(GCC)	4	GCTTCTCCTGATGGTTGGTC	GAATCGAACCCGCATCTTCT	20	20	55.0	50.0	58.3	58.1	0.0	0.0	0.314679	37879	38170	458-459	1.000000	78.7
psbZ-trnG(GCC)_p5	psbZ-trnG(GCC)	5	GGGTTAGTCTTTCTGGTGGG	AATCGAACCCGCATCTTCTC	20	20	55.0	50.0	57.5	58.1	0.0	0.0	0.550350	37879	38170	394-395	1.000000	77.0
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	20	20	50.0	50.0	57.9	58.0	4.1	18.7	0.160416	43542	44285	902-905	1.000000	79.2
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	ATTGGTTGAAGATCACGGGG	20	20	50.0	50.0	57.9	57.9	4.1	0.0	0.244113	43542	44285	854-857	1.000000	78.6
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	TGGTTTGATCAAGCAGCTGA	20	20	50.0	45.0	57.9	57.7	4.1	20.4	0.390477	43542	44285	927-930	1.000000	77.4
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	AGGCTATAGCGCTTACTCCT	20	20	50.0	50.0	57.9	57.7	4.1	18.7	0.411415	43542	44285	896-899	1.000000	77.2
psaA-ycf3_p5	psaA-ycf3	5	GCCCATTCCTCGAAAGAAGT	TTGGAAACAGGCTATAGCGC	20	20	50.0	50.0	57.9	58.3	4.1	18.3	0.451648	43542	44285	904-907	1.000000	76.9
atpB-rbcL_p1	atpB-rbcL	1	ATACGGCCCAGGTTTTGTTT	AACACCCGCTTTGAATCCAA	20	20	45.0	45.0	58.0	58.3	0.0	0.0	0.305431	56402	57207	911-925	1.000000	79.1
atpB-rbcL_p2	atpB-rbcL	2	GGAAACCCCAGAACCAGAAG	AACACCCGCTTTGAATCCAA	20	20	55.0	45.0	58.1	58.3	0.0	0.0	0.390437	56402	57207	882-896	1.000000	78.5
atpB-rbcL_p3	atpB-rbcL	3	GGCGGAAAGGCTACATCTAG	AACACCCGCTTTGAATCCAA	20	20	55.0	45.0	57.8	58.3	0.0	0.0	0.457837	56402	57207	953-967	1.000000	77.8
atpB-rbcL_p4	atpB-rbcL	4	TACGGCCCAGGTTTTGTTTT	AACACCCGCTTTGAATCCAA	20	20	45.0	45.0	58.2	58.3	0.0	0.0	0.523554	56402	57207	910-924	1.000000	77.4
atpB-rbcL_p5	atpB-rbcL	5	GGACCGATGATTTGGACGAT	AACACCCGCTTTGAATCCAA	20	20	50.0	45.0	57.8	58.3	0.0	0.0	0.541987	56402	57207	929-943	1.000000	77.2
rbcL_p1	rbcL	1	AACGCCTAATTCGTGTCGAG	AAGATTGGGCCGAGTTTTCA	20	20	50.0	45.0	58.4	57.7	0.0	0.0	0.660749	57208	58635	1556-1556	1.000000	75.2
rbcL_p2	rbcL	2	AACGCCTAATTCGTGTCGAG	AGATTGGGCCGAGTTTTCAA	20	20	50.0	45.0	58.4	57.7	0.0	0.0	0.660749	57208	58635	1555-1555	1.000000	75.2
rbcL_p3	rbcL	3	CGCCTAATTCGTGTCGAGTA	AAGATTGGGCCGAGTTTTCA	20	20	50.0	45.0	57.5	57.7	0.0	0.0	0.749917	57208	58635	1554-1554	1.000000	74.5
rbcL_p4	rbcL	4	CGCCTAATTCGTGTCGAGTA	AGATTGGGCCGAGTTTTCAA	20	20	50.0	45.0	57.5	57.7	0.0	0.0	0.749917	57208	58635	1553-1553	1.000000	74.5
rbcL_p5	rbcL	5	TAACGCCTAATTCGTGTCGA	AGATTGGGCCGAGTTTTCAA	20	20	45.0	45.0	57.1	57.7	0.0	0.0	1.204065	57208	58635	1556-1556	1.000000	70.9
rbcL-accD_p1	rbcL-accD	1	GAGGCTAGCAAATGGAGTCC	ACCTGCGTTCTAACTCCTTC	20	20	55.0	50.0	58.0	57.3	18.2	0.0	0.785098	58636	59298	838-838	1.000000	74.1
rbcL-accD_p2	rbcL-accD	2	ATTTGAGTTTGCCGCAATGG	ACCTGCGTTCTAACTCCTTC	20	20	45.0	50.0	57.9	57.3	6.6	0.0	0.828681	58636	59298	779-779	1.000000	73.8
rbcL-accD_p3	rbcL-accD	3	GCTGCTTGTGAGGTATGGAA	ACCTGCGTTCTAACTCCTTC	20	20	50.0	50.0	57.9	57.3	0.0	0.0	0.859375	58636	59298	808-808	1.000000	73.5
rbcL-accD_p4	rbcL-accD	4	TGAGTTTGCCGCAATGGATA	ACCTGCGTTCTAACTCCTTC	20	20	45.0	50.0	57.9	57.3	6.6	0.0	0.866806	58636	59298	776-776	1.000000	73.5
rbcL-accD_p5	rbcL-accD	5	AGTTTGCCGCAATGGATACT	ACCTGCGTTCTAACTCCTTC	20	20	45.0	50.0	57.9	57.3	6.6	0.0	0.874285	58636	59298	774-774	1.000000	73.4
ycf4-cemA_p1	ycf4-cemA	1	CATTCCCTTGACCCGTACTG	GAGAAATCCACCAGGGCAAA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.100820	62599	63468	1045-1049	1.000000	79.8
ycf4-cemA_p2	ycf4-cemA	2	ATTCCCTTGACCCGTACTGA	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.129660	62599	63468	1044-1048	1.000000	79.6
ycf4-cemA_p3	ycf4-cemA	3	TTCCCTTGACCCGTACTGAT	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.129660	62599	63468	1043-1047	1.000000	79.6
ycf4-cemA_p4	ycf4-cemA	4	CTTGCGCGTACCAATTGAAG	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.1	58.1	8.4	0.0	0.194446	62599	63468	970-974	1.000000	79.1
ycf4-cemA_p5	ycf4-cemA	5	GAACAAAAAGCCGCCGAATT	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	58.2	58.1	0.0	0.0	0.298310	62599	63468	999-1003	1.000000	78.3
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.9	57.1	6.4	0.0	0.992787	67069	68248	1372-1372	1.000000	72.7
psbE-petL_p2	psbE-petL	2	ACTGACGAATAACCAACCCG	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.9	57.1	0.0	0.0	1.030402	67069	68248	1385-1385	1.000000	72.4
psbE-petL_p3	psbE-petL	3	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	24.9	0.0	1.097796	67069	68248	1295-1295	1.000000	71.9
psbE-petL_p4	psbE-petL	4	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	20	20	45.0	50.0	58.2	57.1	0.0	0.0	1.112474	67069	68248	1381-1381	1.000000	71.7
psbE-petL_p5	psbE-petL	5	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	0.0	0.0	1.128804	67069	68248	1380-1380	1.000000	71.6
rps12-trnV(GAC)_p1	rps12-trnV(GAC)	1	TGGCAACTTTACGTAAGGCA	GATGACTTCCACCACGTCAA	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.421509	100533	102128	1684-1692	1.000000	77.1
rps12-trnV(GAC)_p2	rps12-trnV(GAC)	2	TGGCAACTTTACGTAAGGCA	AACTGATGACTTCCACCACG	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.427523	100533	102128	1688-1696	1.000000	77.1
rps12-trnV(GAC)_p3	rps12-trnV(GAC)	3	TGGCAACTTTACGTAAGGCA	ATGACTTCCACCACGTCAAG	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.427523	100533	102128	1683-1691	1.000000	77.1
rps12-trnV(GAC)_p4	rps12-trnV(GAC)	4	TGGCAACTTTACGTAAGGCA	CTGATGACTTCCACCACGTC	20	20	45.0	55.0	57.7	58.3	16.5	0.0	0.542896	100533	102128	1686-1694	1.000000	76.2
rps12-trnV(GAC)_p5	rps12-trnV(GAC)	5	TGGCAACTTTACGTAAGGCA	GTCAAGGTGACACTCTACCG	20	20	45.0	55.0	57.7	57.7	16.5	5.4	0.606988	100533	102128	1669-1677	1.000000	75.7
ndhF-rpl32_p1	ndhF-rpl32	1	GTTGCCGTCGGAAAAAGAAG	ATGCTTTTAACGCTGTCCCA	20	20	50.0	45.0	58.0	58.1	17.5	0.0	0.128127	114294	114767	666-668	1.000000	79.9
ndhF-rpl32_p2	ndhF-rpl32	2	GTTGCCGTCGGAAAAAGAAG	TGCTTTTAACGCTGTCCCAT	20	20	50.0	45.0	58.0	58.1	17.5	0.0	0.128127	114294	114767	665-667	1.000000	79.9
ndhF-rpl32_p3	ndhF-rpl32	3	TGCCGTCGGAAAAAGAAGAA	ATGCTTTTAACGCTGTCCCA	20	20	45.0	45.0	58.0	58.1	17.5	0.0	0.139416	114294	114767	664-666	1.000000	79.8
ndhF-rpl32_p4	ndhF-rpl32	4	TGCCGTCGGAAAAAGAAGAA	TGCTTTTAACGCTGTCCCAT	20	20	45.0	45.0	58.0	58.1	17.5	0.0	0.139416	114294	114767	663-665	1.000000	79.8
ndhF-rpl32_p5	ndhF-rpl32	5	TTGCCGTCGGAAAAAGAAGA	TGCTTTTAACGCTGTCCCAT	20	20	45.0	45.0	58.0	58.1	17.5	0.0	0.139416	114294	114767	664-666	1.000000	79.8
ycf1_p1	ycf1	1	TGAGTTATTAACATGGAACCCCT	GAGGGGGTCAGCATTCAAAA	23	20	39.1	50.0	57.2	58.1	9.2	0.0	3.866218	128202	128321	186-186	1.000000	51.2
ycf1_p2	ycf1	2	TGAGTTATTAACATGGAACCCCT	AGAAATTGGGTAGGGAGGGG	23	20	39.1	55.0	57.2	58.4	9.2	0.0	4.181149	128202	128321	200-200	1.000000	48.6
ycf1_p3	ycf1	3	TGAGTTATTAACATGGAACCCCTT	GAGGGGGTCAGCATTCAAAA	24	20	37.5	50.0	57.9	58.1	9.2	0.0	4.224081	128202	128321	186-186	1.000000	48.4
ycf1_p4	ycf1	4	TTGAGTTATTAACATGGAACCCCT	GAGGGGGTCAGCATTCAAAA	24	20	37.5	50.0	57.9	58.1	9.2	0.0	4.224081	128202	128321	187-187	1.000000	48.3
ycf1_p5	ycf1	5	TGAGTTATTAACATGGAACCCC	GAGGGGGTCAGCATTCAAAA	22	20	40.9	50.0	55.8	58.1	9.2	0.0	4.306388	128202	128321	186-186	1.000000	47.7