primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	TCACACGTTGCTTTCTACCC	AAGGTTCGAATCCTTCCGTC	20	20	50.0	50.0	58.1	57.6	0.0	19.8	0.508027	6621	7270	293-728	0.923077	84.5
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCACACGTTGCTTTCTACCC	GGTCCCGTTATTCGAAGGTT	20	20	50.0	50.0	58.1	57.6	0.0	3.3	0.517918	6621	7270	307-742	0.923077	84.3
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	ACACGTTGCTTTCTACCCAA	AAGGTTCGAATCCTTCCGTC	20	20	45.0	50.0	57.7	57.6	0.0	19.8	0.717040	6621	7270	291-726	0.923077	82.8
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCACACGTTGCTTTCTACCC	TTGGTCCCGTTATTCGAAGG	20	20	50.0	50.0	58.1	57.6	0.0	3.3	0.516264	6621	7270	309-744	0.846154	80.7
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	ACACGTTGCTTTCTACCCAA	TTGGTCCCGTTATTCGAAGG	20	20	45.0	50.0	57.7	57.6	0.0	3.3	0.725278	6621	7270	307-742	0.846154	79.1
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	AGTCCACTCAGCCATCTCTC	GGGGTCCATAGAACGAATCG	20	20	55.0	55.0	58.5	57.8	0.0	0.0	0.666359	8738	9282	290-625	1.000000	86.7
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AGTCCACTCAGCCATCTCTC	GGTCCATAGAACGAATCGCA	20	20	55.0	50.0	58.5	57.8	0.0	1.2	0.734915	8738	9282	288-623	1.000000	86.1
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	TAGTCCACTCAGCCATCTCT	GGGGTCCATAGAACGAATCG	20	20	50.0	55.0	57.2	57.8	0.0	0.0	0.987033	8738	9282	291-626	1.000000	84.1
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	AGTCCACTCAGCCATCTCTC	GGGTCCATAGAACGAATCGC	20	20	55.0	55.0	58.5	58.5	0.0	1.2	1.016913	8738	9282	289-624	1.000000	83.9
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	TAGTCCACTCAGCCATCTCT	GGTCCATAGAACGAATCGCA	20	20	50.0	50.0	57.2	57.8	0.0	1.2	1.055589	8738	9282	289-624	1.000000	83.6
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	10058	10304	280-346	1.000000	75.3
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	10058	10304	283-349	1.000000	75.1
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	10058	10304	286-352	1.000000	74.7
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	10058	10304	289-355	1.000000	74.5
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.7	56.9	0.0	0.0	2.385293	10058	10304	284-350	1.000000	72.9
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCAGCGGAAAT	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	58.0	57.9	0.0	0.0	0.171127	14432	15147	757-851	1.000000	87.9
atpH-atpI_p2	atpH-atpI	2	ATAACGGAAGCAGCGGAAAT	CCCGTCATGTTCCTTGGATT	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.167157	14432	15147	804-898	1.000000	87.8
atpH-atpI_p3	atpH-atpI	3	TCCCAGGTCCAATAGAAGCA	TTTTGCAACTTTAGCTGCGG	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.167326	14432	15147	801-895	1.000000	87.7
atpH-atpI_p4	atpH-atpI	4	TCCCAGGTCCAATAGAAGCA	CCCGTCATGTTCCTTGGATT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.163356	14432	15147	848-942	1.000000	87.6
atpH-atpI_p5	atpH-atpI	5	TACCTTGACCAATCCCAGGT	CCCGTCATGTTCCTTGGATT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.182517	14432	15147	860-954	1.000000	87.4
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	CTGTTGTATTTGCTTCGCCG	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	58.1	57.8	0.0	1.1	0.287634	38514	39105	737-786	1.000000	87.3
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	TTGTATTTGCTTCGCCGGAT	CGTCTTCTCCTTGGCAAAGA	20	20	45.0	50.0	58.3	57.8	5.0	1.1	0.424600	38514	39105	734-783	1.000000	86.3
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	CTGTTGTATTTGCTTCGCCG	AAAAAGACGGAGGCGGATAG	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.428926	38514	39105	772-821	1.000000	86.0
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	CGGATGGTTGGTCGAGTAAC	CGTCTTCTCCTTGGCAAAGA	20	20	55.0	50.0	58.4	57.8	3.7	1.1	0.532844	38514	39105	719-768	1.000000	85.4
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	CCTGTTGTATTTGCTTCGCC	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.7	57.8	0.0	1.1	0.432404	38514	39105	738-787	0.923077	82.7
rbcL-accD_p1	rbcL-accD	1	AGTCCTGAATTAGCTGCTGC	CTCGATCAACCGGGTGATTT	20	20	50.0	50.0	58.0	58.0	13.4	34.7	0.059706	59242	60960	1957-2957	1.000000	91.5
rbcL-accD_p2	rbcL-accD	2	AGTCCTGAATTAGCTGCTGC	CGGAAGCACACACGAGAATA	20	20	50.0	50.0	58.0	58.0	13.4	0.0	0.044731	59242	60960	2003-2787	0.923077	88.6
rbcL-accD_p3	rbcL-accD	3	AGATCTTTGGCGACGATTCC	CGGAAGCACACACGAGAATA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.067876	59242	60960	2182-2966	0.923077	88.4
rbcL-accD_p4	rbcL-accD	4	AGTCCTGAATTAGCTGCTGC	GATTCCTCCTCCTCCTCCTC	20	20	50.0	60.0	58.0	58.0	13.4	0.0	0.045211	59242	60960	2285-2285	0.076923	42.7
rbcL-accD_p5	rbcL-accD	5	AGATCTTTGGCGACGATTCC	GATTCCTCCTCCTCCTCCTC	20	20	50.0	60.0	58.1	58.0	0.0	0.0	0.068356	59242	60960	2464-2464	0.076923	42.5
accD_p1	accD	1	CGGCTGCGGAAAAAGAATTT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	57.9	58.0	0.0	26.9	0.091577	60961	62037	1578-1578	0.076923	42.3
accD_p2	accD	2	CGGCTGCGGAAAAAGAATTT	ATACGTTCTGATCGCCAACC	20	20	45.0	50.0	57.9	58.1	0.0	8.0	0.128641	60961	62037	1843-1843	0.076923	42.0
accD_p3	accD	3	CGGCTGCGGAAAAAGAATTT	AGATTTGTCTCTACTGCGCG	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.149940	60961	62037	1642-1642	0.076923	41.9
accD_p4	accD	4	CGGCTGCGGAAAAAGAATTT	AAGGAAGCCATTGCAATTGC	20	20	45.0	45.0	57.9	57.9	0.0	24.1	0.168110	60961	62037	1585-1585	0.076923	41.7
accD_p5	accD	5	AGAAATGGTAGTTCGGCTGC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.3	58.0	0.0	26.9	0.287937	60961	62037	1591-1591	0.076923	40.8
clpP_p1	clpP	1	TGCACCAACCGAAAACCATA	GGGGGTGGAGAGTTGATACT	20	20	45.0	55.0	58.0	58.1	0.0	0.0	0.136399	72254	74265	2896-2896	0.076923	42.0
clpP_p2	clpP	2	CTGAACGACTCGCACATACA	GGGGGTGGAGAGTTGATACT	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.141871	72254	74265	2721-2721	0.076923	41.9
clpP_p3	clpP	3	ACATGATCTGAACGACTCGC	GGGGGTGGAGAGTTGATACT	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.204426	72254	74265	2728-2728	0.076923	41.4
clpP_p4	clpP	4	ATGATCTGAACGACTCGCAC	GGGGGTGGAGAGTTGATACT	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.204426	72254	74265	2726-2726	0.076923	41.4
clpP_p5	clpP	5	GATCTGAACGACTCGCACAT	GGGGGTGGAGAGTTGATACT	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.204426	72254	74265	2724-2724	0.076923	41.4
clpP-psbB_p1	clpP-psbB	1	CAGAGCCTGGATGCTTCATT	ACACCCATATTCCAGGACCA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.074423	74266	74811	1342-1508	1.000000	91.4
clpP-psbB_p2	clpP-psbB	2	CAGAGCCTGGATGCTTCATT	AATGCGCCAAAACCAAAACA	20	20	50.0	40.0	57.9	58.0	0.0	4.2	0.084070	74266	74811	1304-1470	1.000000	91.3
clpP-psbB_p3	clpP-psbB	3	CAGAGCCTGGATGCTTCATT	CAGATAGCTGCCAAGAAGCA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.088336	74266	74811	1175-1341	1.000000	91.3
clpP-psbB_p4	clpP-psbB	4	CAGAGCCTGGATGCTTCATT	AGATAGCTGCCAAGAAGCAC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.089762	74266	74811	1174-1340	1.000000	91.3
clpP-psbB_p5	clpP-psbB	5	CAGAGCCTGGATGCTTCATT	TTTGTACAGACGTTGGGGTG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.097935	74266	74811	1518-1684	1.000000	91.2
rps3-rps19_p1	rps3-rps19	1	TACCAAGTCTGAAACCGAGC	GCCTACTCGAAACTTCCTGG	20	20	50.0	55.0	57.5	58.0	0.0	0.0	0.462149	86165	87112	978-1266	0.846154	81.4
rps3-rps19_p2	rps3-rps19	2	TACCAAGTCTGAAACCGAGC	TTCGCCTACTCGAAACTTCC	20	20	50.0	50.0	57.5	57.6	0.0	0.0	0.809548	86165	87112	981-1269	0.846154	78.6
rps3-rps19_p3	rps3-rps19	3	TACCAAGTCTGAAACCGAGC	CGCCTACTCGAAACTTCCTG	20	20	50.0	55.0	57.5	58.4	0.0	0.0	0.820462	86165	87112	979-1267	0.846154	78.5
rps3-rps19_p4	rps3-rps19	4	TACCAAGTCTGAAACCGAGC	TCGCCTACTCGAAACTTCCT	20	20	50.0	50.0	57.5	58.5	0.0	0.0	0.908117	86165	87112	980-1268	0.846154	77.8
rps3-rps19_p5	rps3-rps19	5	TACCAAGTCTGAAACCGAGC	AACTTCCTGGGACATGATGC	20	20	50.0	50.0	57.5	57.9	0.0	0.0	0.585398	86165	87112	968-1256	0.769231	77.1
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	GTTGGGCGCTTTAACCATTC	AATCATGGTCCTTGCGGATC	20	20	50.0	50.0	58.0	58.0	7.6	0.0	0.036329	89625	89959	1026-2107	0.923077	87.5
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	CTTATTGGAGGGTCCCGTTG	AATCATGGTCCTTGCGGATC	20	20	55.0	50.0	58.0	58.0	1.0	0.0	0.065515	89625	89959	1091-2172	0.923077	87.0
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	TAGAACAACCCGGTTGAAGC	AATCATGGTCCTTGCGGATC	20	20	50.0	50.0	58.1	58.0	32.6	0.0	0.140241	89625	89959	1528-2572	0.769231	79.3
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	AGAAGAGTTCGACCCATTGC	AATCATGGTCCTTGCGGATC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.127733	89625	89959	1409-1409	0.076923	42.1
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	GAAGAGTTCGACCCATTGCT	AATCATGGTCCTTGCGGATC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.127733	89625	89959	1408-1408	0.076923	42.1
ndhF-rpl32_p1	ndhF-rpl32	1	AGCAACTGAACCGGCAAATA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.103543	114791	115835	2172-2516	1.000000	90.1
ndhF-rpl32_p2	ndhF-rpl32	2	ATTTAGCAACTGAACCGGCA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.103543	114791	115835	2176-2520	1.000000	90.1
ndhF-rpl32_p3	ndhF-rpl32	3	TAGCAACTGAACCGGCAAAT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.103543	114791	115835	2173-2517	1.000000	90.1
ndhF-rpl32_p4	ndhF-rpl32	4	TAGGGCGTGTGAACCAAAAA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.062062	114791	115835	1963-2307	0.923077	87.6
ndhF-rpl32_p5	ndhF-rpl32	5	TAGGGCGTGTGAACCAAAAA	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.063433	114791	115835	1964-2308	0.923077	87.6
ycf1_p1	ycf1	1	ACCAACGTTTCGGAATCGAT	CCAAACGACAAGAAAAAGGGA	20	21	45.0	42.9	57.9	56.9	23.5	0.0	2.156477	128197	128316	1264-1414	0.846154	68.6
ycf1_p2	ycf1	2	ACCAACGTTTCGGAATCGAT	TCCAAACGACAAGAAAAAGGG	20	21	45.0	42.9	57.9	56.9	23.5	0.0	2.156477	128197	128316	1265-1415	0.846154	68.6
ycf1_p3	ycf1	3	AACCAACGTTTCGGAATCGA	CCAAACGACAAGAAAAAGGGA	20	21	45.0	42.9	58.1	56.9	23.5	0.0	2.199059	128197	128316	1265-1415	0.846154	68.3
ycf1_p4	ycf1	4	CCCGTATTTTGCGTGGTTTC	CCAAACGACAAGAAAAAGGGA	20	21	50.0	42.9	58.0	56.9	0.0	0.0	2.095736	128197	128316	995-1064	0.307692	43.7
ycf1_p5	ycf1	5	CCCGTATTTTGCGTGGTTTC	TCCAAACGACAAGAAAAAGGG	20	21	50.0	42.9	58.0	56.9	0.0	0.0	2.095736	128197	128316	996-1065	0.307692	43.7