primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GTG-psbA_p1	trnH-GTG-psbA	1	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.465225	75	465	466-510	1.000000	84.9
trnH-GTG-psbA_p2	trnH-GTG-psbA	2	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.469587	75	465	480-524	1.000000	84.7
trnH-GTG-psbA_p3	trnH-GTG-psbA	3	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	75	465	479-523	1.000000	84.7
trnH-GTG-psbA_p4	trnH-GTG-psbA	4	ACAATCCACTGCCTTGATCC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	75	465	481-525	1.000000	84.7
trnH-GTG-psbA_p5	trnH-GTG-psbA	5	GATCCACTTGGCTACATCCG	ACCGTGCTAACCTTGGTATG	20	20	55.0	50.0	58.1	57.6	0.0	0.0	0.525510	75	465	467-511	1.000000	84.4
ycf1-rps15_p1	ycf1-rps15	1	AATAATGGGAGCCGCTTGAG	CCGCAGACCTCTCTGAGATA	20	20	50.0	55.0	58.0	58.0	0.0	8.0	0.052863	112245	112644	616-616	0.500000	59.6
ycf1-rps15_p2	ycf1-rps15	2	AGAATCAGAATGGTGCGCAT	CCGCAGACCTCTCTGAGATA	20	20	45.0	55.0	58.0	58.0	15.9	8.0	0.074179	112245	112644	807-807	0.500000	59.4
ycf1-rps15_p3	ycf1-rps15	3	AATAATGGGAGCCGCTTGAG	AACACCATGCATTGATGGGT	20	20	50.0	45.0	58.0	58.1	0.0	8.9	0.085751	112245	112644	893-893	0.500000	59.3
ycf1-rps15_p4	ycf1-rps15	4	AATAATGGGAGCCGCTTGAG	CCCTAATTGCTCTGGTGCAT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.090098	112245	112644	1088-1088	0.500000	59.3
ycf1-rps15_p5	ycf1-rps15	5	AGAATCAGAATGGTGCGCAT	AACACCATGCATTGATGGGT	20	20	45.0	45.0	58.0	58.1	15.9	8.9	0.107067	112245	112644	1084-1084	0.500000	59.1
rps15_p1	rps15	1	AATAATGGGAGCCGCTTGAG	TGAGGTAAATCAGCAGCGAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.037273	112645	112923	1324-1324	0.500000	59.7
rps15_p2	rps15	2	AGAATCAGAATGGTGCGCAT	TGAGGTAAATCAGCAGCGAC	20	20	45.0	50.0	58.0	58.0	15.9	0.0	0.058589	112645	112923	1515-1515	0.500000	59.5
rps15_p3	rps15	3	AATAATGGGAGCCGCTTGAG	AACACCATGCATTGATGGGT	20	20	50.0	45.0	58.0	58.1	0.0	8.9	0.085751	112645	112923	893-893	0.500000	59.3
rps15_p4	rps15	4	AATAATGGGAGCCGCTTGAG	CCCTAATTGCTCTGGTGCAT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.090098	112645	112923	1088-1088	0.500000	59.3
rps15_p5	rps15	5	AGAATCAGAATGGTGCGCAT	AACACCATGCATTGATGGGT	20	20	45.0	45.0	58.0	58.1	15.9	8.9	0.107067	112645	112923	1084-1084	0.500000	59.1
rps15-ndhH_p1	rps15-ndhH	1	AATAATGGGAGCCGCTTGAG	TGAGGTAAATCAGCAGCGAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.037273	112924	113014	1324-1324	0.500000	59.7
rps15-ndhH_p2	rps15-ndhH	2	AATAATGGGAGCCGCTTGAG	AACACCATGCATTGATGGGT	20	20	50.0	45.0	58.0	58.1	0.0	8.9	0.085751	112924	113014	893-893	0.500000	59.3
rps15-ndhH_p3	rps15-ndhH	3	AATAATGGGAGCCGCTTGAG	CCCTAATTGCTCTGGTGCAT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.090098	112924	113014	1088-1088	0.500000	59.3
rps15-ndhH_p4	rps15-ndhH	4	AATAATGGGAGCCGCTTGAG	AGGAGTCTGTGCCCCAATAT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.128265	112924	113014	1208-1208	0.500000	59.0
rps15-ndhH_p5	rps15-ndhH	5	AATAATGGGAGCCGCTTGAG	TTCCCTAATTGCTCTGGTGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.155898	112924	113014	1090-1090	0.500000	58.8
ndhH_p1	ndhH	1	TATGCAAACAACCCGATGCT	CCTGACATAAGAAGGCCGAC	20	20	45.0	55.0	58.2	58.1	0.0	0.0	0.225792	113015	114196	2308-2308	0.500000	58.2
ndhH_p2	ndhH	2	TATGCAAACAACCCGATGCT	GACATAAGAAGGCCGACAGG	20	20	45.0	55.0	58.2	58.1	0.0	0.0	0.225792	113015	114196	2305-2305	0.500000	58.2
ndhH_p3	ndhH	3	AGGTCTGCGGAAAATTCTGG	CCTGACATAAGAAGGCCGAC	20	20	50.0	55.0	58.2	58.1	0.0	0.0	0.232290	113015	114196	1841-1841	0.500000	58.1
ndhH_p4	ndhH	4	AGGTCTGCGGAAAATTCTGG	GACATAAGAAGGCCGACAGG	20	20	50.0	55.0	58.2	58.1	0.0	0.0	0.232290	113015	114196	1838-1838	0.500000	58.1
ndhH_p5	ndhH	5	GTCTGCGGAAAATTCTGGGA	GACATAAGAAGGCCGACAGG	20	20	50.0	55.0	58.2	58.1	0.0	0.0	0.237459	113015	114196	1836-1836	0.500000	58.1
ndhA_p1	ndhA	1	AGAGTTGAAGCACCAAAGGG	TGACCAATATACCTTGCGGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.051324	114204	116358	2507-2507	0.500000	59.6
ndhA_p2	ndhA	2	AGTTGAAGCACCAAAGGGAG	TGACCAATATACCTTGCGGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.051324	114204	116358	2505-2505	0.500000	59.6
ndhA_p3	ndhA	3	GAGTTGAAGCACCAAAGGGA	TGACCAATATACCTTGCGGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.056950	114204	116358	2506-2506	0.500000	59.5
ndhA_p4	ndhA	4	GTTGAAGCACCAAAGGGAGA	TGACCAATATACCTTGCGGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.056950	114204	116358	2504-2504	0.500000	59.5
ndhA_p5	ndhA	5	CAACAGGCGCTAGAAGGAAT	TGACCAATATACCTTGCGGC	20	20	50.0	50.0	58.0	58.0	7.6	0.0	0.068045	114204	116358	2666-2666	0.500000	59.5
ndhI_p1	ndhI	1	TTTGTGACCTTTGAGGAGCC	AGACCACGGAAGTTACTCCA	20	20	50.0	50.0	58.0	58.0	0.1	0.0	0.024114	116435	116935	1875-1875	0.500000	59.8
ndhI_p2	ndhI	2	GTACGGTTCTGCAATAGGGG	AGACCACGGAAGTTACTCCA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.055905	116435	116935	1835-1835	0.500000	59.6
ndhI_p3	ndhI	3	AATAGGGGTGGGAACAGTGA	AGACCACGGAAGTTACTCCA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.059117	116435	116935	1823-1823	0.500000	59.5
ndhI_p4	ndhI	4	GACCTTTGAGGAGCCGTATG	AGACCACGGAAGTTACTCCA	20	20	55.0	50.0	58.1	58.0	0.1	0.0	0.064045	116435	116935	1870-1870	0.500000	59.5
ndhI_p5	ndhI	5	GATGGACTTTACCGAGGCTG	AGACCACGGAAGTTACTCCA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.064045	116435	116935	1364-1364	0.500000	59.5
ndhG_p1	ndhG	1	TGTGAAGTATGTGTTCGCGT	TGGATTGACAGTGCCAAACT	20	20	45.0	45.0	57.9	57.6	5.8	0.0	0.506314	117294	117824	1832-1832	0.500000	55.9
ndhG_p2	ndhG	2	TGTGAAGTATGTGTTCGCGT	GAGTCCAATAGCGGCTTCAG	20	20	45.0	55.0	57.9	58.4	5.8	8.3	0.566652	117294	117824	1635-1635	0.500000	55.5
ndhG_p3	ndhG	3	TGTGAAGTATGTGTTCGCGT	AATAGCGAGTCCAATAGCGG	20	20	45.0	50.0	57.9	57.6	5.8	0.0	0.583547	117294	117824	1641-1641	0.500000	55.3
ndhG_p4	ndhG	4	TCAAATTGCTTTGGGTCGGT	TGGATTGACAGTGCCAAACT	20	20	45.0	45.0	58.3	57.6	0.0	0.0	0.664801	117294	117824	1617-1617	0.500000	54.7
ndhG_p5	ndhG	5	TCAAATTGCTTTGGGTCGGT	GAGTCCAATAGCGGCTTCAG	20	20	45.0	55.0	58.3	58.4	0.0	8.3	0.725138	117294	117824	1420-1420	0.500000	54.2
ndhD_p1	ndhD	1	CATGTGTGCCACGTAAGGTA	GTAGTGATTCACGCGGTTCT	20	20	50.0	50.0	57.9	58.0	0.0	12.5	0.095331	118898	120436	2171-2171	0.500000	59.2
ndhD_p2	ndhD	2	TGTGTGCCACGTAAGGTATG	GTAGTGATTCACGCGGTTCT	20	20	50.0	50.0	57.9	58.0	0.0	12.5	0.095331	118898	120436	2169-2169	0.500000	59.2
ndhD_p3	ndhD	3	CATGTGTGCCACGTAAGGTA	CGCAAAGACCAAGCAGATTG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.114363	118898	120436	2151-2151	0.500000	59.1
ndhD_p4	ndhD	4	TGTGTGCCACGTAAGGTATG	CGCAAAGACCAAGCAGATTG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.114363	118898	120436	2149-2149	0.500000	59.1
ndhD_p5	ndhD	5	TGATACCTTGGGACGGATGT	GTAGTGATTCACGCGGTTCT	20	20	50.0	50.0	58.1	58.0	0.0	12.5	0.133701	118898	120436	1908-1908	0.500000	58.9
ccsA_p1	ccsA	1	AGAACCGCGTGAATCACTAC	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	58.0	58.0	11.9	0.0	0.015661	120639	121613	1249-1249	0.500000	59.9
ccsA_p2	ccsA	2	AGAACCGCGTGAATCACTAC	TGAAATCGGTAGACACGCTG	20	20	50.0	50.0	58.0	58.0	11.9	0.0	0.017031	120639	121613	1248-1248	0.500000	59.9
ccsA_p3	ccsA	3	CAATCTGCTTGGTCTTTGCG	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.034692	120639	121613	1269-1269	0.500000	59.7
ccsA_p4	ccsA	4	CAATCTGCTTGGTCTTTGCG	TGAAATCGGTAGACACGCTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.036063	120639	121613	1268-1268	0.500000	59.7
ccsA_p5	ccsA	5	GTTTTTGATTCTGGACCGCG	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.044083	120639	121613	1498-1498	0.500000	59.6
trnL-TAG-rpl32_p1	trnL-TAG-rpl32	1	AGCGTGTCTACCGATTTCAC	AAAGGATATTGGGCGGCATT	20	20	50.0	45.0	58.0	57.9	0.0	12.5	0.094441	121800	122686	1015-1015	0.500000	59.2
trnL-TAG-rpl32_p2	trnL-TAG-rpl32	2	CAGCGTGTCTACCGATTTCA	AAAGGATATTGGGCGGCATT	20	20	50.0	45.0	58.0	57.9	0.0	12.5	0.095811	121800	122686	1016-1016	0.500000	59.2
trnL-TAG-rpl32_p3	trnL-TAG-rpl32	3	TGATCCACAAAGAAGAGCCG	AAAGGATATTGGGCGGCATT	20	20	50.0	45.0	57.9	57.9	0.0	12.5	0.185752	121800	122686	1640-1640	0.500000	58.5
trnL-TAG-rpl32_p4	trnL-TAG-rpl32	4	CTTAGTGGAAAATGGCCCGA	AAAGGATATTGGGCGGCATT	20	20	50.0	45.0	57.9	57.9	0.0	12.5	0.209394	121800	122686	1378-1378	0.500000	58.3
trnL-TAG-rpl32_p5	trnL-TAG-rpl32	5	TCCTAAGAGCAGCGTGTCTA	AAAGGATATTGGGCGGCATT	20	20	50.0	45.0	57.9	57.9	0.0	12.5	0.210888	121800	122686	1025-1025	0.500000	58.3
rpl32_p1	rpl32	1	GGAAAAGAAGACTCCCCACC	TTTTCACGGATCTTTGACGT	20	20	55.0	40.0	57.8	55.6	0.0	0.0	2.574396	122687	122851	605-605	0.500000	39.4
rpl32_p2	rpl32	2	GGGAAAAGAAGACTCCCCAC	TTTTCACGGATCTTTGACGT	20	20	55.0	40.0	57.8	55.6	0.0	0.0	2.574396	122687	122851	606-606	0.500000	39.4
rpl32_p3	rpl32	3	GGAAAAGAAGACTCCCCACC	TCACGGATCTTTGACGTATCT	20	21	55.0	42.9	57.8	56.6	0.0	0.0	2.621874	122687	122851	602-602	0.500000	39.0
rpl32_p4	rpl32	4	GGGAAAAGAAGACTCCCCAC	TCACGGATCTTTGACGTATCT	20	21	55.0	42.9	57.8	56.6	0.0	0.0	2.621874	122687	122851	603-603	0.500000	39.0
rpl32_p5	rpl32	5	GGTGTCTGGTTTTAGGGCTT	TTTTCACGGATCTTTGACGT	20	20	50.0	40.0	57.7	55.6	0.0	0.0	2.660453	122687	122851	1072-1072	0.500000	38.7
rpl32-ndhF_p1	rpl32-ndhF	1	AATGCCGCCCAATATCCTTT	AGCAATAGCACCGGCAAATA	20	20	45.0	45.0	57.9	57.9	0.0	0.0	0.137945	122852	123838	1790-1790	0.500000	58.9
rpl32-ndhF_p2	rpl32-ndhF	2	AATGCCGCCCAATATCCTTT	ATTTAGCAATAGCACCGGCA	20	20	45.0	45.0	57.9	57.9	0.0	0.0	0.137945	122852	123838	1794-1794	0.500000	58.9
rpl32-ndhF_p3	rpl32-ndhF	3	AATGCCGCCCAATATCCTTT	TAGCAATAGCACCGGCAAAT	20	20	45.0	45.0	57.9	57.9	0.0	0.0	0.137945	122852	123838	1791-1791	0.500000	58.9
rpl32-ndhF_p4	rpl32-ndhF	4	AATGCCGCCCAATATCCTTT	AACAGGTCGTGTGAACCAAA	20	20	45.0	45.0	57.9	57.9	0.0	0.0	0.203437	122852	123838	1583-1583	0.500000	58.4
rpl32-ndhF_p5	rpl32-ndhF	5	AATGCCGCCCAATATCCTTT	ACAGGTCGTGTGAACCAAAA	20	20	45.0	45.0	57.9	57.9	0.0	0.0	0.203437	122852	123838	1582-1582	0.500000	58.4
ndhF_p1	ndhF	1	AGCTAAGATCCGGTGAATCG	CTAGCACTGGGTAGACCTCA	20	20	50.0	55.0	57.2	57.9	0.0	0.0	0.948184	123839	126070	2652-2652	0.500000	52.4
ndhF_p2	ndhF	2	AGCTAAGATCCGGTGAATCG	TCGTTGTGGTCGGACTCTAT	20	20	50.0	50.0	57.2	58.2	0.0	8.2	0.972509	123839	126070	2831-2831	0.500000	52.2
ndhF_p3	ndhF	3	AGCTAAGATCCGGTGAATCG	ATCAATTCGGTCGTTGTGGT	20	20	50.0	45.0	57.2	57.8	0.0	0.0	0.981699	123839	126070	2841-2841	0.500000	52.1
ndhF_p4	ndhF	4	CGAAAGAGCTAAGATCCGGT	CTAGCACTGGGTAGACCTCA	20	20	50.0	55.0	57.1	57.9	0.0	0.0	1.018530	123839	126070	2658-2658	0.500000	51.9
ndhF_p5	ndhF	5	CGAAAGAGCTAAGATCCGGT	TCGTTGTGGTCGGACTCTAT	20	20	50.0	50.0	57.1	58.2	0.0	8.2	1.042854	123839	126070	2837-2837	0.500000	51.7