primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
EI075_pgp60_p1	EI075_pgp60	1	TGGCCTAACAGAGCTTCTCT	TGAGCTTCAGGTCCCCATAA	20	20	50.0	50.0	58.1	58.0	3.3	3.6	0.090409	738	1784	2002-2002	0.500000	59.3
EI075_pgp60_p2	EI075_pgp60	2	CGATCGGTCTCGTTTTAGCA	TGAGCTTCAGGTCCCCATAA	20	20	50.0	50.0	58.1	58.0	7.4	3.6	0.130442	738	1784	2193-2193	0.500000	59.0
EI075_pgp60_p3	EI075_pgp60	3	TGGCCTAACAGAGCTTCTCT	CTGACCAGCCTACGAAAACA	20	20	50.0	50.0	58.1	57.8	3.3	0.0	0.219641	738	1784	1467-1467	0.500000	58.2
EI075_pgp60_p4	EI075_pgp60	4	CGATCGGTCTCGTTTTAGCA	CTGACCAGCCTACGAAAACA	20	20	50.0	50.0	58.1	57.8	7.4	0.0	0.259674	738	1784	1658-1658	0.500000	57.9
EI075_pgp60_p5	EI075_pgp60	5	GCTTTACCCTGGCCTAACAG	TGAGCTTCAGGTCCCCATAA	20	20	55.0	50.0	58.2	58.0	3.3	3.6	0.283149	738	1784	2011-2011	0.500000	57.7
EI075_pgp59_p1	EI075_pgp59	1	ATTCTGCTGCATCAGGATGG	ATTCTTGATCACGACCACCG	20	20	50.0	50.0	58.0	58.0	17.1	0.6	0.027485	1880	3283	2094-2094	0.500000	59.8
EI075_pgp59_p2	EI075_pgp59	2	CTGCTGCATCAGGATGGAAT	ATTCTTGATCACGACCACCG	20	20	50.0	50.0	58.0	58.0	9.8	0.6	0.027485	1880	3283	2091-2091	0.500000	59.8
EI075_pgp59_p3	EI075_pgp59	3	CTGGCTCAGGCAAATAGAGG	ATTCTTGATCACGACCACCG	20	20	55.0	50.0	58.0	58.0	1.0	0.6	0.045593	1880	3283	2173-2173	0.500000	59.6
EI075_pgp59_p4	EI075_pgp59	4	ATTCTGCTGCATCAGGATGG	ATAGCTTCATGTGCTGGCTC	20	20	50.0	50.0	58.0	58.0	17.1	20.4	0.052787	1880	3283	2712-2712	0.500000	59.6
EI075_pgp59_p5	EI075_pgp59	5	CTGCTGCATCAGGATGGAAT	ATAGCTTCATGTGCTGGCTC	20	20	50.0	50.0	58.0	58.0	9.8	20.4	0.052787	1880	3283	2709-2709	0.500000	59.6
EI075_pgp57_p1	EI075_pgp57	1	CGGTGGTCGTGATCAAGAAT	AAAACTTCACCACCAGGACC	20	20	50.0	50.0	58.0	57.9	13.1	0.0	0.077510	3497	4873	1905-2083	1.000000	91.4
EI075_pgp57_p2	EI075_pgp57	2	CGGTGGTCGTGATCAAGAAT	AGCAATCCATCCATCACCAC	20	20	50.0	50.0	58.0	57.9	13.1	0.0	0.073116	3497	4873	2263-2263	0.500000	59.4
EI075_pgp57_p3	EI075_pgp57	3	TGATGGGTGTAGCTGGTGTA	AGCAATCCATCCATCACCAC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.117391	3497	4873	2745-2745	0.500000	59.1
EI075_pgp57_p4	EI075_pgp57	4	CGGTGGTCGTGATCAAGAAT	CACCAAATCCTAGAACGGCA	20	20	50.0	50.0	58.0	57.9	13.1	0.0	0.118095	3497	4873	1973-1973	0.500000	59.1
EI075_pgp57_p5	EI075_pgp57	5	TGATGGGTGTAGCTGGTGTA	AAAACTTCACCACCAGGACC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.121786	3497	4873	2387-2387	0.500000	59.0
EI075_pgp57-trnS(GCU)_p1	EI075_pgp57-trnS(GCU)	1	AGTCTGGTTTGTTTCCTTCT	GTCTGAGTGGCCTAAAGAGC	20	20	40.0	55.0	54.4	58.0	0.0	3.3	3.630769	4874	6445	2370-2370	0.500000	31.0
EI075_pgp57-trnS(GCU)_p2	EI075_pgp57-trnS(GCU)	2	AGTCTGGTTTGTTTCCTTCT	TCTGAGTGGCCTAAAGAGCT	20	20	40.0	50.0	54.4	58.1	0.0	3.3	3.669454	4874	6445	2369-2369	0.500000	30.6
EI075_pgp57-trnS(GCU)_p3	EI075_pgp57-trnS(GCU)	3	AGTCTGGTTTGTTTCCTTCT	CCCTGTCATTCGTAGGTTCG	20	20	40.0	55.0	54.4	58.1	0.0	0.0	3.691061	4874	6445	2431-2431	0.500000	30.5
EI075_pgp57-trnS(GCU)_p4	EI075_pgp57-trnS(GCU)	4	AGTCTGGTTTGTTTCCTTCT	TTTGGCCCTGTCATTCGTAG	20	20	40.0	50.0	54.4	57.9	0.0	0.0	3.721581	4874	6445	2436-2436	0.500000	30.2
EI075_pgp57-trnS(GCU)_p5	EI075_pgp57-trnS(GCU)	5	AGTCTGGTTTGTTTCCTTCT	GGTGTAGCCAAGTGGTAAGG	20	20	40.0	55.0	54.4	57.9	0.0	0.0	3.723056	4874	6445	2464-2464	0.500000	30.2
EI075_pgp42_p1	EI075_pgp42	1	ATTTCCTCTTGCGCCTGAAA	CCAAAACCACAAGTACCGGA	20	20	45.0	50.0	58.1	58.0	4.2	9.5	0.120208	19190	21313	2793-2793	0.500000	59.0
EI075_pgp42_p2	EI075_pgp42	2	ATTTCCTCTTGCGCCTGAAA	TGAAGCATTTGGATGTGCCT	20	20	45.0	45.0	58.1	58.1	4.2	0.0	0.162813	19190	21313	2902-2902	0.500000	58.7
EI075_pgp42_p3	EI075_pgp42	3	TTCCTCTTGCGCCTGAAAAA	CCAAAACCACAAGTACCGGA	20	20	45.0	50.0	58.3	58.0	4.2	9.5	0.343554	19190	21313	2791-2791	0.500000	57.3
EI075_pgp42_p4	EI075_pgp42	4	TTTCCTCTTGCGCCTGAAAA	CCAAAACCACAAGTACCGGA	20	20	45.0	50.0	58.3	58.0	4.2	9.5	0.343554	19190	21313	2792-2792	0.500000	57.3
EI075_pgp42_p5	EI075_pgp42	5	TTCCTCTTGCGCCTGAAAAA	TGAAGCATTTGGATGTGCCT	20	20	45.0	45.0	58.3	58.1	4.2	0.0	0.386158	19190	21313	2900-2900	0.500000	56.9
EI075_pgp40_p1	EI075_pgp40	1	GCGATATGGCCAAGTGGTAA	TTCACCATTGGGCAACTCAA	20	20	50.0	45.0	58.0	57.9	11.0	7.8	0.117376	25133	26083	1623-1623	0.500000	59.1
EI075_pgp40_p2	EI075_pgp40	2	CGAATCTGGGTGTCGTCTTT	TTCACCATTGGGCAACTCAA	20	20	50.0	45.0	57.9	57.9	0.0	7.8	0.159456	25133	26083	1571-1571	0.500000	58.7
EI075_pgp40_p3	EI075_pgp40	3	GCGATATGGCCAAGTGGTAA	CAAGTGCCCCAGACCAATTA	20	20	50.0	50.0	58.0	57.8	11.0	0.0	0.252583	25133	26083	1588-1588	0.500000	58.0
EI075_pgp40_p4	EI075_pgp40	4	CGAATCTGGGTGTCGTCTTT	CAAGTGCCCCAGACCAATTA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.294662	25133	26083	1536-1536	0.500000	57.6
EI075_pgp40_p5	EI075_pgp40	5	GCGATATGGCCAAGTGGTAA	AGCCAAACCTGCAGTTGTAT	20	20	50.0	45.0	58.0	57.7	11.0	13.8	0.331035	25133	26083	1668-1668	0.500000	57.4
EI075_pgp37_p1	EI075_pgp37	1	GGTCAAGGTACTGCATCTGG	CTGGAGCTGGCGATTCTATC	20	20	55.0	55.0	58.0	57.9	0.0	0.0	0.107251	27338	27895	1313-1313	0.500000	59.1
EI075_pgp37_p2	EI075_pgp37	2	GGTCAAGGTACTGCATCTGG	GGAGCTGGCGATTCTATCAG	20	20	55.0	55.0	58.0	57.9	0.0	0.0	0.107251	27338	27895	1311-1311	0.500000	59.1
EI075_pgp37_p3	EI075_pgp37	3	GGCTTAGGTGCTATTGGTCC	CTGGAGCTGGCGATTCTATC	20	20	55.0	55.0	58.0	57.9	0.0	0.0	0.108709	27338	27895	1340-1340	0.500000	59.1
EI075_pgp37_p4	EI075_pgp37	4	GGCTTAGGTGCTATTGGTCC	GGAGCTGGCGATTCTATCAG	20	20	55.0	55.0	58.0	57.9	0.0	0.0	0.108709	27338	27895	1338-1338	0.500000	59.1
EI075_pgp37_p5	EI075_pgp37	5	GGTCAAGGTACTGCATCTGG	TTCGCCTGCCATAACCTTTT	20	20	55.0	45.0	58.0	58.1	0.0	0.0	0.111966	27338	27895	1063-1063	0.500000	59.1
EI075_pgp33_p1	EI075_pgp33	1	GGATGTAGCGCAGTTTGGTA	TTTTGGAAGAAGCTGGTGCA	20	20	50.0	45.0	58.0	58.2	11.5	0.0	0.244432	30771	31350	1402-1402	0.500000	58.0
EI075_pgp33_p2	EI075_pgp33	2	GGATGTAGCGCAGTTTGGTA	TTACAAGTGCAGTTTGGGCA	20	20	50.0	45.0	58.0	58.2	11.5	3.4	0.249516	30771	31350	1183-1183	0.500000	58.0
EI075_pgp33_p3	EI075_pgp33	3	ACGTTTGTTACCGAGGGTTC	TTTTGGAAGAAGCTGGTGCA	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.356116	30771	31350	1277-1277	0.500000	57.2
EI075_pgp33_p4	EI075_pgp33	4	CGTTTGTTACCGAGGGTTCA	TTTTGGAAGAAGCTGGTGCA	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.357421	30771	31350	1276-1276	0.500000	57.1
EI075_pgp33_p5	EI075_pgp33	5	ACGTTTGTTACCGAGGGTTC	TTACAAGTGCAGTTTGGGCA	20	20	50.0	45.0	58.1	58.2	0.0	3.4	0.361200	30771	31350	1058-1058	0.500000	57.1
EI075_pgp24_p1	EI075_pgp24	1	CCATCTGTCGCACTCATTGA	CCAGGCGCTACTTTTCCATA	20	20	50.0	50.0	58.0	57.7	0.0	7.6	0.339918	37181	38287	1889-1889	0.500000	57.3
EI075_pgp24_p2	EI075_pgp24	2	ACTTCACAAACCACTCGCAT	CCAGGCGCTACTTTTCCATA	20	20	45.0	50.0	58.0	57.7	0.0	7.6	0.358264	37181	38287	1832-1832	0.500000	57.1
EI075_pgp24_p3	EI075_pgp24	3	ACCGAACACAGAAACACCTC	CCAGGCGCTACTTTTCCATA	20	20	50.0	50.0	58.1	57.7	0.0	7.6	0.376118	37181	38287	2238-2238	0.500000	57.0
EI075_pgp24_p4	EI075_pgp24	4	CGAACACAGAAACACCTCCA	CCAGGCGCTACTTTTCCATA	20	20	50.0	50.0	58.1	57.7	0.0	7.6	0.377479	37181	38287	2236-2236	0.500000	57.0
EI075_pgp24_p5	EI075_pgp24	5	CACGTGTTCTTTCACCAACG	CCAGGCGCTACTTTTCCATA	20	20	50.0	50.0	57.9	57.7	7.6	7.6	0.411115	37181	38287	2260-2260	0.500000	56.7
EI075_pgp21_p1	EI075_pgp21	1	TATGGAAAAGTAGCGCCTGG	CACGTCGTGCTCAATTAGGA	20	20	50.0	50.0	57.7	58.0	2.3	13.2	0.334784	39107	40054	1617-1617	0.500000	57.3
EI075_pgp21_p2	EI075_pgp21	2	TATGGAAAAGTAGCGCCTGG	TATTCCATTCCGTCGTGCAG	20	20	50.0	50.0	57.7	58.1	2.3	0.0	0.390994	39107	40054	1739-1739	0.500000	56.9
EI075_pgp21_p3	EI075_pgp21	3	TATGGAAAAGTAGCGCCTGG	TTCCGTGATGTATTCGCTGG	20	20	50.0	50.0	57.7	58.1	2.3	0.0	0.390994	39107	40054	1456-1456	0.500000	56.9
EI075_pgp21_p4	EI075_pgp21	4	TATGGAAAAGTAGCGCCTGG	ATTCCGTCGTGCAGAATCAA	20	20	50.0	45.0	57.7	57.9	2.3	0.0	0.418889	39107	40054	1733-1733	0.500000	56.6
EI075_pgp21_p5	EI075_pgp21	5	TATGGAAAAGTAGCGCCTGG	ATGGTGTTGTTCGTGCTGAT	20	20	50.0	45.0	57.7	58.1	2.3	0.0	0.429363	39107	40054	1758-1758	0.500000	56.6
EI075_pgp02_p1	EI075_pgp02	1	GTGTTCCAGAAGTATACGGCT	TGTCGGCAAAACAGCTAGTT	21	20	47.6	45.0	57.5	58.0	0.0	0.0	1.560154	51586	52142	1539-1539	0.500000	47.5
EI075_pgp02_p2	EI075_pgp02	2	GTGTTCCAGAAGTATACGGCT	TTGTCGGCAAAACAGCTAGT	21	20	47.6	45.0	57.5	58.0	0.0	0.0	1.560154	51586	52142	1540-1540	0.500000	47.5
EI075_pgp02_p3	EI075_pgp02	3	GTGTTCCAGAAGTATACGGCT	TTGCGTATTGTTCGTCGACT	21	20	47.6	45.0	57.5	57.9	0.0	14.7	1.576634	51586	52142	1834-1834	0.500000	47.4
EI075_pgp02_p4	EI075_pgp02	4	GTGTTCCAGAAGTATACGGCT	CCTGGTCAGCATGGTTCAAT	21	20	47.6	50.0	57.5	58.2	0.0	0.0	1.678579	51586	52142	1765-1765	0.500000	46.6
EI075_pgp02_p5	EI075_pgp02	5	GTGTTCCAGAAGTATACGGCT	TCTTGTCGGCAAAACAGCTA	21	20	47.6	45.0	57.5	57.8	0.0	3.1	1.770227	51586	52142	1542-1542	0.500000	45.8
EI075_pgp01_p1	EI075_pgp01	1	CCAGATCCTGTGCCACTAAC	GGGACACCTGGTGGAAAATT	20	20	55.0	50.0	58.0	58.0	0.0	29.1	0.039520	52199	52810	1226-1226	0.500000	59.7
EI075_pgp01_p2	EI075_pgp01	2	CTTCCTGCAGACGACCATAC	GGGACACCTGGTGGAAAATT	20	20	55.0	50.0	58.1	58.0	17.4	29.1	0.081786	52199	52810	1097-1097	0.500000	59.3
EI075_pgp01_p3	EI075_pgp01	3	CCAGATCCTGTGCCACTAAC	GAGCGGTAGTACGTTCCAAG	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.124389	52199	52810	1708-1708	0.500000	59.0
EI075_pgp01_p4	EI075_pgp01	4	CCAGATCCTGTGCCACTAAC	ACTAGCGCCATGACAAAACA	20	20	55.0	45.0	58.0	58.1	0.0	2.3	0.138962	52199	52810	1203-1203	0.500000	58.9
EI075_pgp01_p5	EI075_pgp01	5	CCAGATCCTGTGCCACTAAC	ACCGAAAAATACACACCGCT	20	20	55.0	45.0	58.0	58.1	0.0	0.0	0.142952	52199	52810	1567-1567	0.500000	58.9