primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	rps8-rpl14	1	ACTCGAACCACGCTTTTTCT	GAACTCGAGTTTTTGGTGCG	20	20	45.0	50.0	58.1	58.0	0.0	30.2	0.069252	79123	79346	350-387	1.000000	89.7
rps8-rpl14_p2	rps8-rpl14	2	ACTCGAACCACGCTTTTTCT	CGATTGCCCGAGAATTGAGA	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.110560	79123	79346	332-369	1.000000	89.7
rps8-rpl14_p3	rps8-rpl14	3	ACTCGAACCACGCTTTTTCT	TTGCCCGAGAATTGAGACAG	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.149676	79123	79346	329-366	1.000000	89.5
rps8-rpl14_p4	rps8-rpl14	4	ACTCGAACCACGCTTTTTCT	GCCCGAGAATTGAGACAGTT	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.151139	79123	79346	327-364	1.000000	89.5
rps8-rpl14_p5	rps8-rpl14	5	ACTCGAACCACGCTTTTTCT	ACTCGAGTTTTTGGTGCGAT	20	20	45.0	45.0	58.1	58.1	0.0	13.6	0.171582	79123	79346	348-385	1.000000	89.0
ndhF_p1	ndhF	1	CTAGCACTGGGTAGACCTCA	TGTCGCTTGGTCATATCATTTG	20	22	55.0	40.9	57.9	57.1	0.0	0.0	3.026283	107672	109897	2758-2758	0.500000	35.8
ndhF_p2	ndhF	2	TCGTTGTGGTCGGACTCTAT	TGTCGCTTGGTCATATCATTTG	20	22	50.0	40.9	58.2	57.1	8.2	0.0	3.050608	107672	109897	2946-2946	0.500000	35.6
ndhF_p3	ndhF	3	ATCAATTCGGTCGTTGTGGT	TGTCGCTTGGTCATATCATTTG	20	22	45.0	40.9	57.8	57.1	0.0	0.0	3.059798	107672	109897	2956-2956	0.500000	35.5
ndhF_p4	ndhF	4	TCTCTTCCTTCTCCGAGCTC	TGTCGCTTGGTCATATCATTTG	20	22	55.0	40.9	58.2	57.1	2.7	0.0	3.123204	107672	109897	2888-2888	0.500000	35.0
ndhF_p5	ndhF	5	CTAGCACTGGGTAGACCTCA	GCCCAAATTTTTATCCTTGCTCT	20	23	55.0	39.1	57.9	57.9	0.0	0.0	3.235367	107672	109897	2876-2876	0.500000	34.1
ndhF-rpl32_p1	ndhF-rpl32	1	GTTGCCGTCGGAAAAAGAAG	ATATCTGCCCTTCCCGAGAA	20	20	50.0	50.0	58.0	57.9	17.5	0.0	0.135568	109898	110958	1584-1584	0.500000	58.9
ndhF-rpl32_p2	ndhF-rpl32	2	TGCCGTCGGAAAAAGAAGAA	ATATCTGCCCTTCCCGAGAA	20	20	45.0	50.0	58.0	57.9	17.5	0.0	0.146857	109898	110958	1582-1582	0.500000	58.8
ndhF-rpl32_p3	ndhF-rpl32	3	TTGCCGTCGGAAAAAGAAGA	ATATCTGCCCTTCCCGAGAA	20	20	45.0	50.0	58.0	57.9	17.5	0.0	0.146857	109898	110958	1583-1583	0.500000	58.8
ndhF-rpl32_p4	ndhF-rpl32	4	TACTTTTTGTTGCCGTCGGA	ATATCTGCCCTTCCCGAGAA	20	20	45.0	50.0	58.0	57.9	17.5	0.0	0.152325	109898	110958	1592-1592	0.500000	58.8
ndhF-rpl32_p5	ndhF-rpl32	5	GTTGCCGTCGGAAAAAGAAG	TGCCCTTCCCGAGAAAGATA	20	20	50.0	50.0	58.0	58.1	17.5	0.0	0.158285	109898	110958	1579-1579	0.500000	58.7
rpl32_p1	rpl32	1	CCAATTTTCACGGATCTTTGACA	ATATCTGCCCTTCCCGAGAA	23	20	39.1	50.0	57.9	57.9	0.0	0.0	3.223084	110959	111123	586-586	0.500000	34.2
rpl32_p2	rpl32	2	CCAATTTTCACGGATCTTTGACA	TATCTGCCCTTCCCGAGAAA	23	20	39.1	50.0	57.9	58.1	0.0	0.0	3.245801	110959	111123	585-585	0.500000	34.0
rpl32_p3	rpl32	3	CCAATTTTCACGGATCTTTGACA	TGCCCTTCCCGAGAAAGATA	23	20	39.1	50.0	57.9	58.1	0.0	0.0	3.245801	110959	111123	581-581	0.500000	34.0
rpl32_p4	rpl32	4	GGGCTAAAAAGAGTACTTGATGC	ATATCTGCCCTTCCCGAGAA	23	20	43.5	50.0	57.8	57.9	0.0	0.0	3.339808	110959	111123	1174-1174	0.500000	33.3
rpl32_p5	rpl32	5	GGGCTAAAAAGAGTACTTGATGC	TGCCCTTCCCGAGAAAGATA	23	20	43.5	50.0	57.8	58.1	0.0	0.0	3.362525	110959	111123	1169-1169	0.500000	33.1
rpl32-trnL_p1	rpl32-trnL	1	GGCAGCGTTAAAAGCTTTGT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.9	58.0	13.5	0.0	0.142032	111124	111927	921-921	0.500000	58.9
rpl32-trnL_p2	rpl32-trnL	2	GGCAGCGTTAAAAGCTTTGT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.9	58.0	13.5	0.0	0.143402	111124	111927	922-922	0.500000	58.9
rpl32-trnL_p3	rpl32-trnL	3	AAAGGATATTGGGCAGCGTT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.151706	111124	111927	932-932	0.500000	58.8
rpl32-trnL_p4	rpl32-trnL	4	AAAGGATATTGGGCAGCGTT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.153076	111124	111927	933-933	0.500000	58.8
rpl32-trnL_p5	rpl32-trnL	5	GGCAGCGTTAAAAGCTTTGT	GAAGTAGCAAAGCCTTGGGT	20	20	45.0	50.0	57.9	58.1	13.5	0.0	0.223144	111124	111927	1432-1432	0.500000	58.2
trnL-ccsA_p1	trnL-ccsA	1	GTGAAATCGGTAGACACGCT	GAAGTAGCAAAGCCTTGGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.096773	112008	112123	531-531	0.500000	59.2
trnL-ccsA_p2	trnL-ccsA	2	GTGAAATCGGTAGACACGCT	TGAAGTAGCAAAGCCTTGGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.098131	112008	112123	532-532	0.500000	59.2
trnL-ccsA_p3	trnL-ccsA	3	TGAAATCGGTAGACACGCTG	GAAGTAGCAAAGCCTTGGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.098143	112008	112123	530-530	0.500000	59.2
trnL-ccsA_p4	trnL-ccsA	4	TGAAATCGGTAGACACGCTG	TGAAGTAGCAAAGCCTTGGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.099501	112008	112123	531-531	0.500000	59.2
trnL-ccsA_p5	trnL-ccsA	5	GTGAAATCGGTAGACACGCT	TGATCCACAAAGAAGAGCCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.106972	112008	112123	649-649	0.500000	59.1
ccsA_p1	ccsA	1	GTGAAATCGGTAGACACGCT	GTTTTTGATTCTGGACCGCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.044083	112124	113092	1564-1564	0.500000	59.6
ccsA_p2	ccsA	2	TGAAATCGGTAGACACGCTG	GTTTTTGATTCTGGACCGCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.045454	112124	113092	1563-1563	0.500000	59.6
ccsA_p3	ccsA	3	GTGAAATCGGTAGACACGCT	CGAGAACCACGTGAATCACT	20	20	50.0	50.0	58.0	57.9	0.0	19.3	0.073823	112124	113092	1277-1277	0.500000	59.4
ccsA_p4	ccsA	4	TGAAATCGGTAGACACGCTG	CGAGAACCACGTGAATCACT	20	20	50.0	50.0	58.0	57.9	0.0	19.3	0.075193	112124	113092	1276-1276	0.500000	59.4
ccsA_p5	ccsA	5	GTGAAATCGGTAGACACGCT	GCATTACCGGGTATGAGTGG	20	20	50.0	55.0	58.0	58.1	0.0	18.9	0.128877	112124	113092	1765-1765	0.500000	59.0
ndhD_p1	ndhD	1	GGGCTCATATTGGAGTTGGG	TCTGCTCCAAGAACAGAGGA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.028574	113357	114877	2229-2229	0.500000	59.8
ndhD_p2	ndhD	2	GGGGCTCATATTGGAGTTGG	TCTGCTCCAAGAACAGAGGA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.028574	113357	114877	2230-2230	0.500000	59.8
ndhD_p3	ndhD	3	AGTGATTCACGTGGTTCTCG	TCTGCTCCAAGAACAGAGGA	20	20	50.0	50.0	57.9	58.0	17.0	0.0	0.081449	113357	114877	1905-1905	0.500000	59.3
ndhD_p4	ndhD	4	TCGTGTGATTAGCCTAGGGT	TCTGCTCCAAGAACAGAGGA	20	20	50.0	50.0	57.8	58.0	19.2	0.0	0.170023	113357	114877	2304-2304	0.500000	58.6
ndhD_p5	ndhD	5	GGGCTCATATTGGAGTTGGG	CAGAGGATTGTGTCGGTTGT	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.182950	113357	114877	2216-2216	0.500000	58.5
ndhG_p1	ndhG	1	CTTCTGGATTGACAGTGCCA	TTGATCACATCCGAGCGTTT	20	20	50.0	45.0	57.8	57.9	0.0	0.0	0.282900	115989	116519	1898-1898	0.500000	57.7
ndhG_p2	ndhG	2	ACTTCTGGATTGACAGTGCC	TTGATCACATCCGAGCGTTT	20	20	50.0	45.0	57.8	57.9	0.0	0.0	0.284434	115989	116519	1899-1899	0.500000	57.7
ndhG_p3	ndhG	3	ATGATCATACCTTGCGTGGC	TTGATCACATCCGAGCGTTT	20	20	50.0	45.0	58.4	57.9	6.4	0.0	0.495564	115989	116519	1847-1847	0.500000	56.0
ndhG_p4	ndhG	4	CTTCTGGATTGACAGTGCCA	AATTGATCACATCCGAGCGT	20	20	50.0	45.0	57.8	57.7	0.0	0.0	0.505519	115989	116519	1900-1900	0.500000	56.0
ndhG_p5	ndhG	5	CTTCTGGATTGACAGTGCCA	ATTGATCACATCCGAGCGTT	20	20	50.0	45.0	57.8	57.7	0.0	0.0	0.505519	115989	116519	1899-1899	0.500000	56.0
ndhG-ndhI_p1	ndhG-ndhI	1	AGACCACGGAAGTTACTCCA	GATGGACTTTACCGAGGCTG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.064045	116520	116873	1362-1362	0.500000	59.5
ndhG-ndhI_p2	ndhG-ndhI	2	AGACTTGTACAGACCACGGA	GATGGACTTTACCGAGGCTG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.067554	116520	116873	1372-1372	0.500000	59.5
ndhG-ndhI_p3	ndhG-ndhI	3	AGACCACGGAAGTTACTCCA	TTGATCACATCCGAGCGTTT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.098676	116520	116873	1027-1027	0.500000	59.2
ndhG-ndhI_p4	ndhG-ndhI	4	AGACTTGTACAGACCACGGA	TTGATCACATCCGAGCGTTT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.102185	116520	116873	1037-1037	0.500000	59.2
ndhG-ndhI_p5	ndhG-ndhI	5	AGACCACGGAAGTTACTCCA	AGATGGACTTTACCGAGGCT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.138007	116520	116873	1363-1363	0.500000	58.9
ndhI_p1	ndhI	1	AGACCACGGAAGTTACTCCA	AGCCGAGTGTGAACGATTAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.013201	116874	117374	1667-1667	0.500000	59.9
ndhI_p2	ndhI	2	AGACTTGTACAGACCACGGA	AGCCGAGTGTGAACGATTAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.016709	116874	117374	1677-1677	0.500000	59.9
ndhI_p3	ndhI	3	AGACCACGGAAGTTACTCCA	GGTTCTGCAATAGGGATGGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.018488	116874	117374	1829-1829	0.500000	59.9
ndhI_p4	ndhI	4	AGACTTGTACAGACCACGGA	GGTTCTGCAATAGGGATGGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.021997	116874	117374	1839-1839	0.500000	59.8
ndhI_p5	ndhI	5	AGACCACGGAAGTTACTCCA	GATGGACTTTACCGAGGCTG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.064045	116874	117374	1362-1362	0.500000	59.5
ndhA_p1	ndhA	1	TGACCAATATACCTTGCGGC	AATCCGCCGCTTTGATTTTG	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.043291	117453	119618	2632-2632	0.500000	59.7
ndhA_p2	ndhA	2	TGACCAATATACCTTGCGGC	TCAACAGGCCCTAGAAGGAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.077069	117453	119618	2680-2680	0.500000	59.4
ndhA_p3	ndhA	3	TGACCAATATACCTTGCGGC	TTCAACAGGCCCTAGAAGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.077069	117453	119618	2681-2681	0.500000	59.4
ndhA_p4	ndhA	4	TGACCAATATACCTTGCGGC	GGAGTAGGCATTCTTGGTGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.082329	117453	119618	2892-2892	0.500000	59.3
ndhA_p5	ndhA	5	AAACGCTCGGATGTGATCAA	AATCCGCCGCTTTGATTTTG	20	20	45.0	45.0	57.9	58.0	0.0	0.0	0.098390	117453	119618	2719-2719	0.500000	59.2
ndhH_p1	ndhH	1	CGTTGTTGTATACCCGCAGA	GGCAGTTGGGTATTCGAGAG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.059627	119620	120804	1494-1494	0.500000	59.5
ndhH_p2	ndhH	2	CGTTGTTGTATACCCGCAGA	GGGAGCCGTTTGAGTATGTT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.110376	119620	120804	1986-1986	0.500000	59.1
ndhH_p3	ndhH	3	CCTGACATAAGAAGGCCGAC	GGCAGTTGGGTATTCGAGAG	20	20	55.0	55.0	58.1	58.1	0.0	0.0	0.117350	119620	120804	1758-1758	0.500000	59.1
ndhH_p4	ndhH	4	GACATAAGAAGGCCGACAGG	GGCAGTTGGGTATTCGAGAG	20	20	55.0	55.0	58.1	58.1	0.0	0.0	0.117350	119620	120804	1755-1755	0.500000	59.1
ndhH_p5	ndhH	5	CGTTGTTGTATACCCGCAGA	TAGGGCAGTTGGGTATTCGA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.132239	119620	120804	1497-1497	0.500000	58.9
ndhH-rps15_p1	ndhH-rps15	1	TGAGGTAAATCAGCAGCGAC	GGCAGTTGGGTATTCGAGAG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.068820	120805	120895	629-629	0.500000	59.4
ndhH-rps15_p2	ndhH-rps15	2	CCACCAAGAATGCCTACTCC	GGCAGTTGGGTATTCGAGAG	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.102798	120805	120895	755-755	0.500000	59.2
ndhH-rps15_p3	ndhH-rps15	3	AACACCATGCATTGATGGGT	GGCAGTTGGGTATTCGAGAG	20	20	45.0	55.0	58.1	58.1	8.9	0.0	0.117298	120805	120895	198-198	0.500000	59.1
ndhH-rps15_p4	ndhH-rps15	4	TGAGGTAAATCAGCAGCGAC	GGGAGCCGTTTGAGTATGTT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.119570	120805	120895	1121-1121	0.500000	59.0
ndhH-rps15_p5	ndhH-rps15	5	CCTAATTGCTCTGGCCCATT	GGCAGTTGGGTATTCGAGAG	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.136362	120805	120895	392-392	0.500000	58.9
rps15-ycf1_p1	rps15-ycf1	1	CCGCAGACCTCTCTGAGATA	AATTATGGAACCTGCCCGTC	20	20	55.0	50.0	58.0	57.9	8.0	0.0	0.078517	121175	121380	897-897	0.500000	59.4
rps15-ycf1_p2	rps15-ycf1	2	AACACCATGCATTGATGGGT	AATTATGGAACCTGCCCGTC	20	20	45.0	50.0	58.1	57.9	8.9	0.0	0.111405	121175	121380	1171-1171	0.500000	59.1
rps15-ycf1_p3	rps15-ycf1	3	CTCTCGAATACCCAACTGCC	AATTATGGAACCTGCCCGTC	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.111457	121175	121380	993-993	0.500000	59.1
rps15-ycf1_p4	rps15-ycf1	4	CCGCAGACCTCTCTGAGATA	TATGGAACCTGCCCGTCTAT	20	20	55.0	50.0	58.0	57.9	8.0	0.0	0.122845	121175	121380	894-894	0.500000	59.0
rps15-ycf1_p5	rps15-ycf1	5	CCTAATTGCTCTGGCCCATT	AATTATGGAACCTGCCCGTC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.130470	121175	121380	1365-1365	0.500000	59.0
ycf1_p1	ycf1	1	TTGTAGAGCTATTCTGCGCA	AACAACCAGGAAGACGCAAT	20	20	45.0	45.0	57.0	58.0	11.0	0.0	0.978251	123917	124036	945-945	0.500000	52.2
ycf1_p2	ycf1	2	TTGTAGAGCTATTCTGCGCA	GACCGTCTCAAGGAAATCCC	20	20	45.0	55.0	57.0	58.0	11.0	0.0	0.985096	123917	124036	1112-1112	0.500000	52.1
ycf1_p3	ycf1	3	TTGTAGAGCTATTCTGCGCA	GGACCGTCTCAAGGAAATCC	20	20	45.0	55.0	57.0	58.0	11.0	0.0	0.985096	123917	124036	1113-1113	0.500000	52.1
ycf1_p4	ycf1	4	TTGTAGAGCTATTCTGCGCA	GGGACCGTCTCAAGGAAATC	20	20	45.0	55.0	57.0	58.0	11.0	0.0	0.985096	123917	124036	1114-1114	0.500000	52.1
ycf1_p5	ycf1	5	TTGTAGAGCTATTCTGCGCA	AGGCATAATTCAAGGGCTCG	20	20	45.0	50.0	57.0	58.0	11.0	0.0	0.986635	123917	124036	1342-1342	0.500000	52.1