primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score rps16-trnQ-UUG_p1 rps16-trnQ-UUG 1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 20 20 45.0 55.0 58.1 58.1 8.1 16.9 0.215425 5581 6479 727-976 1.000000 88.4 rps16-trnQ-UUG_p2 rps16-trnQ-UUG 2 TCATCTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 57.6 58.1 0.0 16.9 0.446382 5581 6479 738-987 1.000000 86.5 rps16-trnQ-UUG_p3 rps16-trnQ-UUG 3 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 20 20 45.0 50.0 58.1 57.3 8.1 16.9 0.806797 5581 6479 731-980 1.000000 83.7 rps16-trnQ-UUG_p4 rps16-trnQ-UUG 4 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 57.2 58.1 0.0 16.9 0.863824 5581 6479 719-968 1.000000 83.3 rps16-trnQ-UUG_p5 rps16-trnQ-UUG 5 AGTCGCACGTTGCTTTCTAC GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 58.9 58.1 8.1 16.9 0.938588 5581 6479 726-975 1.000000 82.6 psbI-trnS-GCU_p1 psbI-trnS-GCU 1 AATCCTGGACGTGAGGAGTA TAAAGCGGCGGATTGCTAAT 20 20 50.0 45.0 57.5 58.0 16.1 9.8 0.565094 7595 7714 209-209 1.000000 77.0 psbI-trnS-GCU_p2 psbI-trnS-GCU 2 ATCCTGGACGTGAGGAGTAA TAAAGCGGCGGATTGCTAAT 20 20 50.0 45.0 57.5 58.0 16.1 9.8 0.565094 7595 7714 208-208 1.000000 77.0 psbI-trnS-GCU_p3 psbI-trnS-GCU 3 TAATCCTGGACGTGAGGAGT TAAAGCGGCGGATTGCTAAT 20 20 50.0 45.0 57.5 58.0 16.1 9.8 0.565094 7595 7714 210-210 1.000000 77.0 psbI-trnS-GCU_p4 psbI-trnS-GCU 4 ATCCTGGACGTGAGGAGTAA CTTTCGATTTGGAACGGGGA 20 20 50.0 50.0 57.5 58.2 16.1 0.0 0.710813 7595 7714 245-245 1.000000 75.6 psbI-trnS-GCU_p5 psbI-trnS-GCU 5 ATCCTGGACGTGAGGAGTAA TTTCGATTTGGAACGGGGAG 20 20 50.0 50.0 57.5 58.2 16.1 0.0 0.710813 7595 7714 244-244 1.000000 75.6 trnG-UCC-trnT-GGU_p1 trnG-UCC-trnT-GGU 1 ACGAATCACACTTTTACCACT CTACCGCTAAGGGAAAAGCC 21 20 38.1 55.0 55.5 58.3 0.0 0.0 3.870969 13434 14267 948-973 1.000000 53.8 trnG-UCC-trnT-GGU_p2 trnG-UCC-trnT-GGU 2 AGAACGAATCACACTTTTACCAC CTACCGCTAAGGGAAAAGCC 23 20 39.1 55.0 57.3 58.3 0.0 0.0 4.003685 13434 14267 951-976 1.000000 52.7 trnG-UCC-trnT-GGU_p3 trnG-UCC-trnT-GGU 3 GAACGAATCACACTTTTACCACT CTACCGCTAAGGGAAAAGCC 23 20 39.1 55.0 57.3 58.3 0.0 0.0 4.003685 13434 14267 950-975 1.000000 52.7 trnG-UCC-trnT-GGU_p4 trnG-UCC-trnT-GGU 4 AACGAATCACACTTTTACCACT CTACCGCTAAGGGAAAAGCC 22 20 36.4 55.0 56.2 58.3 0.0 0.0 4.111440 13434 14267 949-974 1.000000 51.9 trnG-UCC-trnT-GGU_p5 trnG-UCC-trnT-GGU 5 ACGAATCACACTTTTACCACT TCTACCGCTAAGGGAAAAGC 21 20 38.1 50.0 55.5 57.3 0.0 0.0 4.221692 13434 14267 949-974 1.000000 51.0 trnY-GUA-trnD-GUC_p1 trnY-GUA-trnD-GUC 1 AATTCTTCCTGGGTCGATGC GCGGTGCTCTAACCAATTGA 20 20 50.0 50.0 58.0 58.6 0.0 7.1 0.595138 14929 15282 422-479 1.000000 81.7 trnY-GUA-trnD-GUC_p2 trnY-GUA-trnD-GUC 2 GGGGACGGACTGTAAATTCG GCGGTGCTCTAACCAATTGA 20 20 55.0 50.0 58.4 58.6 0.0 7.1 0.902511 14929 15282 389-446 1.000000 79.4 trnY-GUA-trnD-GUC_p3 trnY-GUA-trnD-GUC 3 GGGACGGACTGTAAATTCGT GCGGTGCTCTAACCAATTGA 20 20 50.0 50.0 57.6 58.6 0.0 7.1 0.926188 14929 15282 388-445 1.000000 79.2 trnY-GUA-trnD-GUC_p4 trnY-GUA-trnD-GUC 4 AATTCTTCCTGGGTCGATGC GGCGGTGCTCTAACCAATT 20 19 50.0 52.6 58.0 58.1 0.0 0.0 1.175870 14929 15282 423-480 1.000000 77.0 trnY-GUA-trnD-GUC_p5 trnY-GUA-trnD-GUC 5 TGGGGACGGACTGTAAATTC GCGGTGCTCTAACCAATTGA 20 20 50.0 50.0 57.2 58.6 0.0 7.1 1.325008 14929 15282 390-447 1.000000 76.0 trnD-GUC-psbM_p1 trnD-GUC-psbM 1 TCAATTGGTTAGAGCACCGC TCATTTTGGCTGGCTGTTTT 20 20 50.0 40.0 58.6 56.7 5.4 2.0 1.848735 15357 16096 895-948 1.000000 69.2 trnD-GUC-psbM_p2 trnD-GUC-psbM 2 TAGGTATGCCATACACCCCG TCATTTTGGCTGGCTGTTTT 20 20 55.0 40.0 58.7 56.7 16.3 2.0 1.953655 15357 16096 928-981 1.000000 68.2 trnD-GUC-psbM_p3 trnD-GUC-psbM 3 TCAATTGGTTAGAGCACCGC AATCATTTTGGCTGGCTGTT 20 20 50.0 40.0 58.6 56.5 5.4 2.0 2.080552 15357 16096 897-950 1.000000 67.3 trnD-GUC-psbM_p4 trnD-GUC-psbM 4 TCAATTGGTTAGAGCACCGC ATCATTTTGGCTGGCTGTTT 20 20 50.0 40.0 58.6 56.5 5.4 2.0 2.080552 15357 16096 896-949 1.000000 67.3 trnD-GUC-psbM_p5 trnD-GUC-psbM 5 TCAATTGGTTAGAGCACCGC TCATTTTGGCTGGCTGTTTTT 20 21 50.0 38.1 58.6 57.4 5.4 2.0 2.108244 15357 16096 895-948 1.000000 67.1 psbM-petN_p1 psbM-petN 1 AAAACAGCCAGCCAAAATGA AGTATGGGGGAGAAGTGGAC 20 20 40.0 55.0 56.7 58.1 0.0 0.0 1.416779 16202 17038 863-886 1.000000 75.1 psbM-petN_p2 psbM-petN 2 AAAACAGCCAGCCAAAATGA GTATGGGGGAGAAGTGGACT 20 20 40.0 55.0 56.7 58.1 0.0 0.0 1.416779 16202 17038 862-885 1.000000 75.1 psbM-petN_p3 psbM-petN 3 AAAACAGCCAGCCAAAATGA TATGGGGGAGAAGTGGACTC 20 20 40.0 55.0 56.7 57.8 0.0 0.0 1.473967 16202 17038 861-884 1.000000 74.6 psbM-petN_p4 psbM-petN 4 AAAACAGCCAGCCAAAATGA GGGGGAGAAGTGGACTCTAG 20 20 40.0 60.0 56.7 58.2 0.0 0.0 1.514649 16202 17038 858-881 1.000000 74.3 psbM-petN_p5 psbM-petN 5 AACAGCCAGCCAAAATGATT GTATGGGGGAGAAGTGGACT 20 20 40.0 55.0 56.5 58.1 0.0 0.0 1.648595 16202 17038 860-883 1.000000 73.2 trnC-GCA-rpoB_p1 trnC-GCA-rpoB 1 AAAGGATTTGCAGTCCCCTG CCGGGTATTGTGGACATTCC 20 20 50.0 55.0 58.1 58.3 14.6 0.0 0.395580 17929 19004 1127-1160 1.000000 83.2 trnC-GCA-rpoB_p2 trnC-GCA-rpoB 2 AAAGGATTTGCAGTCCCCTG CGGGTATTGTGGACATTCCC 20 20 50.0 55.0 58.1 58.3 14.6 0.0 0.395580 17929 19004 1126-1159 1.000000 83.2 trnC-GCA-rpoB_p3 trnC-GCA-rpoB 3 AAAGGATTTGCAGTCCCCTG TCCCTCATTTCCATTCCGGA 20 20 50.0 50.0 58.1 58.4 14.6 0.0 0.480541 17929 19004 1110-1143 1.000000 82.7 trnC-GCA-rpoB_p4 trnC-GCA-rpoB 4 AAAGGATTTGCAGTCCCCTG TTCCCTCATTTCCATTCCGG 20 20 50.0 50.0 58.1 57.6 14.6 0.0 0.506504 17929 19004 1111-1144 1.000000 82.4 trnC-GCA-rpoB_p5 trnC-GCA-rpoB 5 AAAGGATTTGCAGTCCCCTG TCCGGGTATTGTGGACATTC 20 20 50.0 50.0 58.1 57.3 14.6 0.0 0.773724 17929 19004 1128-1161 1.000000 80.2 atpI-atpH_p1 atpI-atpH 1 TAGCCGCAGCCTATATAGGT CTCTACAGCTTGTCCTGCAG 20 20 50.0 55.0 57.5 58.0 26.3 6.8 0.534248 31064 31879 964-974 1.000000 77.7 atpI-atpH_p2 atpI-atpH 2 ATTTTTGCAACGTTAGCCGC CTCTACAGCTTGTCCTGCAG 20 20 45.0 55.0 58.6 58.0 16.5 6.8 0.592983 31064 31879 977-987 1.000000 77.2 atpI-atpH_p3 atpI-atpH 3 TAGCCGCAGCCTATATAGGT AGCCAATCCAGCAGCAATAA 20 20 50.0 45.0 57.5 57.9 26.3 0.0 0.675333 31064 31879 901-911 1.000000 76.7 atpI-atpH_p4 atpI-atpH 4 TAGCCGCAGCCTATATAGGT GCAGTACCTTGACCAACTCC 20 20 50.0 55.0 57.5 58.2 26.3 0.0 0.717204 31064 31879 945-955 1.000000 76.3 atpI-atpH_p5 atpI-atpH 5 TAGCCGCAGCCTATATAGGT AAGCAGCAGCAATTAGTGGA 20 20 50.0 45.0 57.5 57.8 26.3 3.2 0.731910 31064 31879 878-888 1.000000 76.3 ycf3-trnS-GGA_p1 ycf3-trnS-GGA 1 TCTCCTGAGGTTGTCGGAAT ACGGAAAGAGAGGGATTCGA 20 20 50.0 50.0 58.1 58.2 0.0 0.0 0.212764 43734 44312 747-766 1.000000 81.2 ycf3-trnS-GGA_p2 ycf3-trnS-GGA 2 TCTCCTGAGGTTGTCGGAAT TTCCAATGCTACGCCTTGAA 20 20 50.0 45.0 58.1 57.8 0.0 5.6 0.254079 43734 44312 697-716 1.000000 81.1 ycf3-trnS-GGA_p3 ycf3-trnS-GGA 3 TTCTCCTGAGGTTGTCGGAA ACGGAAAGAGAGGGATTCGA 20 20 50.0 50.0 58.3 58.2 0.0 0.0 0.439292 43734 44312 748-767 1.000000 79.4 ycf3-trnS-GGA_p4 ycf3-trnS-GGA 4 TTTCTCCTGAGGTTGTCGGA ACGGAAAGAGAGGGATTCGA 20 20 50.0 50.0 58.3 58.2 0.0 0.0 0.439292 43734 44312 749-768 1.000000 79.4 ycf3-trnS-GGA_p5 ycf3-trnS-GGA 5 TTCTCCTGAGGTTGTCGGAA TTCCAATGCTACGCCTTGAA 20 20 50.0 45.0 58.3 57.8 0.0 5.6 0.480608 43734 44312 698-717 1.000000 79.3 trnT-UGU-trnL-UAA_p1 trnT-UGU-trnL-UAA 1 ATTTGAACCGATGACCCTCG ACAATCAAGTCCGTAGCGTC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.032006 45649 46459 899-913 1.000000 84.2 trnT-UGU-trnL-UAA_p2 trnT-UGU-trnL-UAA 2 CGATGACCCTCGCATTACAA ACAATCAAGTCCGTAGCGTC 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.073242 45649 46459 891-905 1.000000 83.9 trnT-UGU-trnL-UAA_p3 trnT-UGU-trnL-UAA 3 GCGATGCTCTAACCTCTGAG ACAATCAAGTCCGTAGCGTC 20 20 55.0 50.0 57.9 58.0 0.0 0.0 0.140570 45649 46459 869-883 1.000000 83.4 trnT-UGU-trnL-UAA_p4 trnT-UGU-trnL-UAA 4 TCCTCGATAACGGGACATGA ACAATCAAGTCCGTAGCGTC 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.069340 45649 46459 1231-1242 1.000000 83.0 trnT-UGU-trnL-UAA_p5 trnT-UGU-trnL-UAA 5 ATTTGAACCGATGACCCTCG GCTTCCATTGAGTCTCTGCA 20 20 50.0 50.0 58.0 57.9 0.0 0.6 0.129182 45649 46459 1068-1082 1.000000 82.8 trnF-GAA-ndhJ_p1 trnF-GAA-ndhJ 1 CCTCGTGTCACCAGTTCAAA ATAGGCTGGCCCTTACGTAA 20 20 50.0 50.0 58.1 58.2 0.0 7.9 0.254575 47521 48062 634-663 1.000000 81.4 trnF-GAA-ndhJ_p2 trnF-GAA-ndhJ 2 ATGCACAAGAAAGGGTCAGG ATAGGCTGGCCCTTACGTAA 20 20 50.0 50.0 58.1 58.2 0.0 7.9 0.287539 47521 48062 687-716 1.000000 80.9 trnF-GAA-ndhJ_p3 trnF-GAA-ndhJ 3 GCACAAGAAAGGGTCAGGAT ATAGGCTGGCCCTTACGTAA 20 20 50.0 50.0 57.8 58.2 0.0 7.9 0.409256 47521 48062 685-714 1.000000 79.9 trnF-GAA-ndhJ_p4 trnF-GAA-ndhJ 4 CCTCGTGTCACCAGTTCAAA GAAAGTTGGATAGGCTGGCC 20 20 50.0 55.0 58.1 58.6 0.0 3.0 0.658293 47521 48062 643-672 1.000000 78.2 trnF-GAA-ndhJ_p5 trnF-GAA-ndhJ 5 AATCCTCGTGTCACCAGTTC ATAGGCTGGCCCTTACGTAA 20 20 50.0 50.0 57.5 58.2 0.0 7.9 0.660045 47521 48062 637-666 1.000000 78.2 ndhC-trnV-UAC_p1 ndhC-trnV-UAC 1 TCTGGTCCTTCACTACTCGG GGTTCGAATCCGTATAGCCC 20 20 55.0 55.0 58.2 57.9 0.0 0.0 0.240485 49747 50656 1048-1051 1.000000 79.5 ndhC-trnV-UAC_p2 ndhC-trnV-UAC 2 TCTGGTCCTTCACTACTCGG AGCCCAATGCAGAAAAATGC 20 20 55.0 45.0 58.2 57.9 0.0 0.0 0.268043 49747 50656 1141-1144 1.000000 79.2 ndhC-trnV-UAC_p3 ndhC-trnV-UAC 3 CACTACTCGGGGCTAAAAGC GGTTCGAATCCGTATAGCCC 20 20 55.0 55.0 58.3 57.9 0.0 0.0 0.422828 49747 50656 1038-1041 1.000000 78.1 ndhC-trnV-UAC_p4 ndhC-trnV-UAC 4 CACTACTCGGGGCTAAAAGC AGCCCAATGCAGAAAAATGC 20 20 55.0 45.0 58.3 57.9 0.0 0.0 0.450386 49747 50656 1131-1134 1.000000 77.7 ndhC-trnV-UAC_p5 ndhC-trnV-UAC 5 TCTGGTCCTTCACTACTCGG CGGTTCGAATCCGTATAGCC 20 20 55.0 55.0 58.2 58.3 0.0 22.8 0.469866 49747 50656 1049-1052 1.000000 77.7 psbE-petL_p1 psbE-petL 1 TGACAAATAACCAACCCGCA AAGCAGCCAGTAGAAAACCG 20 20 45.0 50.0 58.0 58.5 0.0 0.0 0.493582 61649 62738 1248-1255 1.000000 77.5 psbE-petL_p2 psbE-petL 2 TGACAAATAACCAACCCGCA GCAGCCAGTAGAAAACCGAA 20 20 45.0 50.0 58.0 58.5 0.0 0.0 0.497931 61649 62738 1246-1253 1.000000 77.5 psbE-petL_p3 psbE-petL 3 CAACCCGCAATGAATAGGGA AAGCAGCCAGTAGAAAACCG 20 20 50.0 50.0 57.9 58.5 6.4 0.0 0.527577 61649 62738 1237-1244 1.000000 77.3 psbE-petL_p4 psbE-petL 4 CAACCCGCAATGAATAGGGA GCAGCCAGTAGAAAACCGAA 20 20 50.0 50.0 57.9 58.5 6.4 0.0 0.531926 61649 62738 1235-1242 1.000000 77.2 psbE-petL_p5 psbE-petL 5 ACCAACCCGCAATGAATAGG AAGCAGCCAGTAGAAAACCG 20 20 50.0 50.0 58.2 58.5 0.0 0.0 0.713535 61649 62738 1239-1246 1.000000 75.8 clpP_p1 clpP 1 GTTTAACCGTTGGCATGTCG TATGCACCGAAAGCAATCGA 20 20 50.0 45.0 58.0 58.0 0.0 12.1 0.058721 66944 67597 1372-1518 1.000000 82.6 clpP_p2 clpP 2 CTGAACGAGTCGCACATACA TATGCACCGAAAGCAATCGA 20 20 50.0 45.0 58.0 58.0 13.6 12.1 0.039950 66944 67597 1485-1631 1.000000 82.5 clpP_p3 clpP 3 CACCCTAGCACATGTTCCTC TATGCACCGAAAGCAATCGA 20 20 55.0 45.0 58.0 58.0 0.0 12.1 0.046976 66944 67597 1467-1613 1.000000 82.5 clpP_p4 clpP 4 CCGTTGGCATGTCGTATGTA TATGCACCGAAAGCAATCGA 20 20 50.0 45.0 58.1 58.0 0.0 12.1 0.087908 66944 67597 1366-1512 1.000000 82.4 clpP_p5 clpP 5 CCTAGCACATGTTCCTCGAC TATGCACCGAAAGCAATCGA 20 20 55.0 45.0 58.1 58.0 0.0 12.1 0.089243 66944 67597 1464-1610 1.000000 82.2 infA-rps8_p1 infA-rps8 1 ACTTTTGCTTCTCTCGGGTT CCTAAGGTTTTGGGCGGAAT 20 20 45.0 50.0 57.4 58.2 0.0 0.0 0.792988 75798 75876 236-250 1.000000 79.0 infA-rps8_p2 infA-rps8 2 ACTTTTGCTTCTCTCGGGTT ATTCCTAAGGTTTTGGGCGG 20 20 45.0 50.0 57.4 58.2 0.0 0.0 0.792988 75798 75876 239-253 1.000000 78.9 infA-rps8_p3 infA-rps8 3 ACTTTTGCTTCTCTCGGGTT TTTTGGGCGGAATGGGAATT 20 20 45.0 45.0 57.4 58.3 0.0 0.0 0.989788 75798 75876 229-243 1.000000 77.4 infA-rps8_p4 infA-rps8 4 ACTTTTGCTTCTCTCGGGTT GGCGGAATGGGAATTGCTAT 20 20 45.0 50.0 57.4 58.4 0.0 0.0 1.019493 75798 75876 224-238 1.000000 77.2 infA-rps8_p5 infA-rps8 5 ACTTTTGCTTCTCTCGGGTT TAAGGTTTTGGGCGGAATGG 20 20 45.0 50.0 57.4 58.4 0.0 0.0 1.088864 75798 75876 234-248 1.000000 76.6 ndhF-rpl32_p1 ndhF-rpl32 1 GGAAGTGGGAGAAGAGGGAT TGACATCGAAGTACGTTTTT 20 20 55.0 35.0 58.1 53.2 0.0 0.0 4.880333 102960 103869 990-996 1.000000 43.6 ndhF-rpl32_p2 ndhF-rpl32 2 GGAAGTGGGAGAAGAGGGAT TTGACATCGAAGTACGTTTT 20 20 55.0 35.0 58.1 53.2 0.0 0.0 4.880333 102960 103869 991-997 1.000000 43.6 ndhF-rpl32_p3 ndhF-rpl32 3 GGAAGTGGGAGAAGAGGGAT TTTGACATCGAAGTACGTTT 20 20 55.0 35.0 58.1 53.2 0.0 0.0 4.880333 102960 103869 992-998 1.000000 43.6 ndhF-rpl32_p4 ndhF-rpl32 4 GGAAGTGGGAGAAGAGGGAT TTTTGACATCGAAGTACGTT 20 20 55.0 35.0 58.1 53.2 0.0 0.0 4.880333 102960 103869 993-999 1.000000 43.6 ndhF-rpl32_p5 ndhF-rpl32 5 GGAAGTGGGAGAAGAGGGAT TTTTTGACATCGAAGTACGT 20 20 55.0 35.0 58.1 53.2 0.0 0.0 4.880333 102960 103869 994-1000 1.000000 43.6 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 ACCAAAAGGTTTTTCTGGGT TCCTAAGAGCAGCGTGTCTA 20 20 40.0 50.0 55.5 57.9 0.0 0.0 2.587216 104035 104563 516-590 1.000000 66.9 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 ACCAAAAGGTTTTTCTGGGT AGCAGCGTGTCTACCAATTT 20 20 40.0 45.0 55.5 57.8 0.0 0.0 2.647943 104035 104563 509-583 1.000000 66.5 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 ACCAAAAGGTTTTTCTGGGT GCTTGAGCACTGCTTCCTAA 20 20 40.0 50.0 55.5 58.2 0.0 4.7 2.649426 104035 104563 530-604 1.000000 66.4 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 ACCAAAAGGTTTTTCTGGGT CCTAAGAGCAGCGTGTCTAC 20 20 40.0 55.0 55.5 57.8 0.0 0.0 2.659234 104035 104563 515-589 1.000000 66.4 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 ACCAAAAGGTTTTTCTGGGT CTAAGAGCAGCGTGTCTACC 20 20 40.0 55.0 55.5 57.8 0.0 0.0 2.659234 104035 104563 514-588 1.000000 66.4