primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.465225	77	349	369-391	1.000000	82.6
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.469587	77	349	383-405	1.000000	82.5
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	77	349	382-404	1.000000	82.5
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	77	349	384-406	1.000000	82.4
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	ACCGTGCTAACCTTGGTATG	20	20	55.0	50.0	58.1	57.6	0.0	0.0	0.525510	77	349	370-392	1.000000	82.1
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCGATGGTATGGACGAATCC	GCCGTACGAGGAGAAAACTT	20	20	55.0	50.0	57.9	57.9	0.0	18.8	0.156265	4198	4852	1046-1156	1.000000	81.0
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	TGGATACCTCGGAAACAGGT	GCCGTACGAGGAGAAAACTT	20	20	50.0	50.0	58.1	57.9	0.0	18.8	0.128995	4198	4852	1588-1699	1.000000	80.9
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TGGATACCTCGGAAACAGGT	ACTGGGCCAGACTTAAGGAT	20	20	50.0	50.0	58.1	58.0	0.0	39.0	0.080428	4198	4852	1880-1960	0.800000	71.8
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCGATGGTATGGACGAATCC	ACTGGGCCAGACTTAAGGAT	20	20	55.0	50.0	57.9	58.0	0.0	39.0	0.107698	4198	4852	1338-1418	0.800000	71.6
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	ACTGGGCCAGACTTAAGGAT	20	20	50.0	50.0	58.2	58.0	20.3	39.0	0.195832	4198	4852	1276-1356	0.800000	70.9
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5964	7221	1265-1331	1.000000	85.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5964	7221	1276-1342	1.000000	84.8
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	5964	7221	1269-1335	1.000000	80.6
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.7	8.1	0.0	0.818817	5964	7221	1295-1361	1.000000	80.5
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	ATCATGTCCTTCAAGTCGCA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.2	58.1	0.0	16.9	0.844354	5964	7221	1277-1343	1.000000	80.3
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	20	21	45.0	38.1	58.0	55.5	0.0	0.0	3.574049	8265	8841	651-737	1.000000	58.1
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	CATCAAGGGAAGCGGAAAGA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	57.9	55.5	0.0	0.0	3.649703	8265	8841	711-797	1.000000	57.2
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	TCATCAAGGGAAGCGGAAAG	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	57.9	55.5	0.0	0.0	3.649703	8265	8841	712-798	1.000000	57.2
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	ATTAGCAATCCGCCGCTTTA	AACGAATCACACTTTTACCACT	20	22	45.0	36.4	58.0	56.2	0.0	0.0	3.814520	8265	8841	652-738	1.000000	56.2
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	CAAGGGAAGCGGAAAGAGAG	ACGAATCACACTTTTACCACT	20	21	55.0	38.1	58.3	55.5	0.0	0.0	3.804123	8265	8841	708-794	1.000000	56.0
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	GGCTTATATCGGCGAATCCA	20	20	45.0	50.0	58.0	57.6	1.4	15.9	0.418471	13393	14596	1151-1273	1.000000	82.2
atpH-atpI_p2	atpH-atpI	2	TACCCTCTACAGCTTGACCC	GGCTTATATCGGCGAATCCA	20	20	55.0	50.0	58.1	57.6	0.0	15.9	0.518102	13393	14596	1234-1356	1.000000	81.3
atpH-atpI_p3	atpH-atpI	3	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	20	20	45.0	45.0	58.0	58.5	1.4	0.0	0.556109	13393	14596	1170-1292	1.000000	81.1
atpH-atpI_p4	atpH-atpI	4	AATAACGGAAGCGGCAGAAA	GGCTTATATCGGCGAATCCA	20	20	45.0	50.0	58.2	57.6	1.4	15.9	0.564009	13393	14596	1152-1274	1.000000	81.0
atpH-atpI_p5	atpH-atpI	5	TACCCTCTACAGCTTGACCC	TTTTTGCAACTTTAGCCGCG	20	20	55.0	45.0	58.1	58.5	0.0	0.0	0.655741	13393	14596	1253-1375	1.000000	80.2
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	GAAAAGACCGGATCTCCACC	GGGAAATACTTCGGCGAGTT	20	20	55.0	50.0	58.0	58.0	9.5	10.5	0.045536	26798	28010	2070-2079	0.800000	72.2
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	CGAGCCCATATCCTTGCTTT	GGGAAATACTTCGGCGAGTT	20	20	50.0	50.0	58.0	58.0	0.0	10.5	0.047076	26798	28010	1863-1872	0.800000	72.2
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	GAAAAGACCGGATCTCCACC	CGCTTCTGCTGGGAAATACT	20	20	55.0	50.0	58.0	58.0	9.5	0.0	0.055427	26798	28010	2080-2089	0.800000	72.1
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	CGAGCCCATATCCTTGCTTT	CGCTTCTGCTGGGAAATACT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.056966	26798	28010	1873-1882	0.800000	72.1
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	ACCGAAATTCCGCTATGGTC	GGGAAATACTTCGGCGAGTT	20	20	50.0	50.0	58.1	58.0	0.0	10.5	0.070482	26798	28010	1785-1794	0.800000	72.1
petN-psbM_p1	petN-psbM	1	ACTCGTAGTGTGGGGAAGAA	TCATTCTAGTTCCTACCGCCT	20	21	50.0	47.6	58.0	58.0	0.0	0.0	1.033465	28804	29995	1230-1288	1.000000	77.1
petN-psbM_p2	petN-psbM	2	TCGTAGTGTGGGGAAGAAGT	TCATTCTAGTTCCTACCGCCT	20	21	50.0	47.6	58.0	58.0	0.0	0.0	1.033465	28804	29995	1228-1286	1.000000	77.1
petN-psbM_p3	petN-psbM	3	ACTCGTAGTGTGGGGAAGAA	TCTAGTTCCTACCGCCTTTCT	20	21	50.0	47.6	58.0	57.9	0.0	0.0	1.102712	28804	29995	1226-1284	1.000000	76.5
petN-psbM_p4	petN-psbM	4	TCGTAGTGTGGGGAAGAAGT	TCTAGTTCCTACCGCCTTTCT	20	21	50.0	47.6	58.0	57.9	0.0	0.0	1.102712	28804	29995	1224-1282	1.000000	76.5
petN-psbM_p5	petN-psbM	5	CGTAGTGTGGGGAAGAAGTG	TCATTCTAGTTCCTACCGCCT	20	21	55.0	47.6	57.9	58.0	0.0	0.0	1.108380	28804	29995	1227-1285	1.000000	76.5
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	ATCAAGCTGCCGAGTATTGG	20	20	50.0	50.0	57.9	58.0	4.1	0.0	0.156301	41701	42425	903-921	1.000000	80.8
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	CGATCAAGCTGCCGAGTATT	20	20	50.0	50.0	57.9	58.1	4.1	0.0	0.257251	41701	42425	905-923	1.000000	80.0
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	20	20	50.0	45.0	57.9	57.8	4.1	0.0	0.279161	41701	42425	832-850	1.000000	80.0
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	20	20	50.0	50.0	57.9	58.2	4.1	0.0	0.306932	41701	42425	836-854	1.000000	79.7
psaA-ycf3_p5	psaA-ycf3	5	GCCCATTCCTCGAAAGAAGT	AGCTGCCGAGTATTGGAAAC	20	20	50.0	50.0	57.9	58.3	4.1	0.0	0.376276	41701	42425	899-917	1.000000	79.0
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	CTCCCTTTCTCCCGAAGTTG	CCCTCGGTAAACAAAAGCCT	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.194967	44462	44993	685-714	1.000000	83.6
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	CTCCCTTTCTCCCGAAGTTG	TTCCAATGCTACGCCTTGAA	20	20	55.0	45.0	57.9	57.8	0.0	5.6	0.293268	44462	44993	656-685	1.000000	83.0
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	CCCTTTCTCCCGAAGTTGTC	CCCTCGGTAAACAAAAGCCT	20	20	55.0	50.0	58.2	58.1	0.0	0.0	0.284679	44462	44993	683-712	1.000000	82.9
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	CCCTTTCTCCCGAAGTTGTC	TTCCAATGCTACGCCTTGAA	20	20	55.0	45.0	58.2	57.8	0.0	5.6	0.382980	44462	44993	654-683	1.000000	82.3
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	CTCCCTTTCTCCCGAAGTTG	ACCCTCGGTAAACAAAAGCC	20	20	55.0	50.0	57.9	58.4	0.0	0.0	0.487928	44462	44993	686-715	1.000000	81.2
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCATATTCGTAAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	22	20	31.8	55.0	53.9	58.7	0.0	0.0	6.805875	50569	51670	733-1146	1.000000	46.1
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TCATATTCGTAAAGCAGAAACA	TTCGAGTCCGTATAGCCCTA	22	20	31.8	50.0	53.9	57.1	0.0	0.0	7.059445	50569	51670	732-1145	1.000000	46.1
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	ATCATATTCGTAAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	23	20	30.4	55.0	54.5	58.7	0.0	0.0	7.219731	50569	51670	734-1147	1.000000	46.1
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TCATATTCGTAAAGCAGAAACAT	GTTCGAGTCCGTATAGCCCT	23	20	30.4	55.0	54.5	58.7	0.0	0.0	7.219731	50569	51670	733-1146	1.000000	46.1
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CATATTCGTAAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	21	20	33.3	55.0	52.3	58.7	0.0	0.0	7.384064	50569	51670	732-1145	1.000000	46.1
psaI-ycf4_p1	psaI-ycf4	1	TTTCCGGCAATTGCAATGTC	GGATAAAGGCCCAGCAGAAA	20	20	45.0	50.0	57.9	57.9	30.3	0.0	0.221288	59829	60264	573-578	1.000000	82.0
psaI-ycf4_p2	psaI-ycf4	2	TTTCCGGCAATTGCAATGTC	AAAGGATAAAGGCCCAGCAG	20	20	45.0	50.0	57.9	57.9	30.3	0.0	0.227386	59829	60264	576-581	1.000000	81.9
psaI-ycf4_p3	psaI-ycf4	3	TAGGCCTAGTATTTCCGGCA	GGATAAAGGCCCAGCAGAAA	20	20	50.0	50.0	57.9	57.9	8.9	0.0	0.234974	59829	60264	584-589	1.000000	81.8
psaI-ycf4_p4	psaI-ycf4	4	TAGGCCTAGTATTTCCGGCA	AAAGGATAAAGGCCCAGCAG	20	20	50.0	50.0	57.9	57.9	8.9	0.0	0.241071	59829	60264	587-592	1.000000	81.7
psaI-ycf4_p5	psaI-ycf4	5	GGCCTAGTATTTCCGGCAAT	GGATAAAGGCCCAGCAGAAA	20	20	50.0	50.0	57.7	57.9	4.4	0.0	0.415819	59829	60264	582-587	1.000000	80.4
petA-psbJ_p1	petA-psbJ	1	ATCCATTACGTGTCCAAGGC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	58.0	57.6	0.0	4.1	0.403359	63380	64087	852-859	1.000000	81.4
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.8	57.6	0.0	4.1	0.519826	63380	64087	835-842	1.000000	80.6
petA-psbJ_p3	petA-psbJ	3	CATTACGTGTCCAAGGCCTT	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	58.2	57.6	13.8	4.1	0.543756	63380	64087	849-856	1.000000	80.3
petA-psbJ_p4	petA-psbJ	4	TTACGTGTCCAAGGCCTTTT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	57.6	57.6	17.6	4.1	0.716678	63380	64087	847-854	1.000000	79.0
petA-psbJ_p5	petA-psbJ	5	TACGTGTCCAAGGCCTTTTG	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	58.4	57.6	17.6	4.1	0.764949	63380	64087	846-853	1.000000	78.6
psbF-psbE_p1	psbF-psbE	1	GAACAGCCAACCATCGTACT	AACTGGCCGTTTTGATCCTT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.099386	64605	64613	116-116	1.000000	79.9
psbF-psbE_p2	psbF-psbE	2	GAACAGCCAACCATCGTACT	ACTGGCCGTTTTGATCCTTT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.099386	64605	64613	115-115	1.000000	79.9
psbF-psbE_p3	psbF-psbE	3	AACAGCCAACCATCGTACTG	AACTGGCCGTTTTGATCCTT	20	20	50.0	45.0	58.2	58.0	0.0	0.0	0.196213	64605	64613	115-115	1.000000	79.1
psbF-psbE_p4	psbF-psbE	4	AACAGCCAACCATCGTACTG	ACTGGCCGTTTTGATCCTTT	20	20	50.0	45.0	58.2	58.0	0.0	0.0	0.196213	64605	64613	114-114	1.000000	79.1
psbF-psbE_p5	psbF-psbE	5	TGAACAGCCAACCATCGTAC	ACTGGCCGTTTTGATCCTTT	20	20	50.0	45.0	58.2	58.0	0.0	0.0	0.201290	64605	64613	116-116	1.000000	79.1
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	CAATTGTGACCTCTCGGGAG	TGGGGGAACTAGAGGATTGG	20	20	55.0	55.0	58.0	58.1	4.9	0.0	0.122421	108753	110403	2045-2206	1.000000	84.5
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	AGAATGATGGGGATAGCGGA	TGGGGGAACTAGAGGATTGG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.136712	108753	110403	2300-2461	1.000000	84.3
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	GCGAGCCCCTTTATTCTGAA	TGGGGGAACTAGAGGATTGG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.145072	108753	110403	1815-1976	1.000000	84.3
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	AAGCGAGCCCCTTTATTCTG	TGGGGGAACTAGAGGATTGG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.150808	108753	110403	1817-1978	1.000000	84.2
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	TTGTGACCTCTCGGGAGAAT	TGGGGGAACTAGAGGATTGG	20	20	50.0	55.0	58.1	58.1	15.7	0.0	0.168100	108753	110403	2042-2203	1.000000	84.1
ndhF-rpl32_p1	ndhF-rpl32	1	GGTATGAGCCACCCGTATTG	CGCTGTCCAATATCCCTTCC	20	20	55.0	55.0	58.1	58.1	0.0	0.0	0.236812	112633	113322	809-847	1.000000	81.3
ndhF-rpl32_p2	ndhF-rpl32	2	ATAGGAACGGGAAGTGGGAT	CGCTGTCCAATATCCCTTCC	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.307746	112633	113322	833-871	1.000000	80.7
ndhF-rpl32_p3	ndhF-rpl32	3	GAACGGGAAGTGGGATGAAA	CGCTGTCCAATATCCCTTCC	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.309966	112633	113322	829-867	1.000000	80.7
ndhF-rpl32_p4	ndhF-rpl32	4	AAGGTATGAGCCACCCGTAT	CGCTGTCCAATATCCCTTCC	20	20	50.0	55.0	58.2	58.1	0.0	0.0	0.313211	112633	113322	811-849	1.000000	80.7
ndhF-rpl32_p5	ndhF-rpl32	5	AGGTATGAGCCACCCGTATT	CGCTGTCCAATATCCCTTCC	20	20	50.0	55.0	58.2	58.1	0.0	0.0	0.313211	112633	113322	810-848	1.000000	80.7
ndhA_p1	ndhA	1	CGTGAGACAGGGTAATCGTG	AGAGTTGAAGCCCCAAAAGG	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.091700	119405	121605	2584-2593	1.000000	81.3
ndhA_p2	ndhA	2	TATGTATCGGGCAGCTCGTA	AGAGTTGAAGCCCCAAAAGG	20	20	50.0	50.0	58.1	58.0	15.9	0.0	0.100830	119405	121605	2553-2562	1.000000	81.3
ndhA_p3	ndhA	3	CGTGAGACAGGGTAATCGTG	TCCAACCTTTGAATTGGCGA	20	20	55.0	45.0	58.1	58.0	0.0	0.0	0.102331	119405	121605	2621-2630	1.000000	81.2
ndhA_p4	ndhA	4	CGTGAGACAGGGTAATCGTG	GTGAGAGTTGAAGCCCCAAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.109368	119405	121605	2587-2596	1.000000	81.2
ndhA_p5	ndhA	5	TATGTATCGGGCAGCTCGTA	TCCAACCTTTGAATTGGCGA	20	20	50.0	45.0	58.1	58.0	15.9	0.0	0.111461	119405	121605	2590-2599	1.000000	81.1
ycf1_p1	ycf1	1	GCAAAGCCACTTCGTTTCTC	AAAGCGCCGTTTATGAGGAT	20	20	50.0	45.0	58.0	58.0	0.0	2.3	0.076328	127213	127332	1059-1065	1.000000	82.9
ycf1_p2	ycf1	2	GCAAAGCCACTTCGTTTCTC	AAGCGCCGTTTATGAGGATT	20	20	50.0	45.0	58.0	58.0	0.0	2.3	0.076328	127213	127332	1058-1064	1.000000	82.9
ycf1_p3	ycf1	3	AGCGCGCATAGAACCTTTAA	GCCTTTTTCCAGATCCCGAT	20	20	45.0	50.0	58.0	57.9	35.1	0.0	0.077135	127213	127332	503-509	1.000000	82.9
ycf1_p4	ycf1	4	AGCGCGCATAGAACCTTTAA	AAAGCGCCGTTTATGAGGAT	20	20	45.0	45.0	58.0	58.0	35.1	2.3	0.056929	127213	127332	1118-1124	1.000000	82.8
ycf1_p5	ycf1	5	AGCGCGCATAGAACCTTTAA	AAGCGCCGTTTATGAGGATT	20	20	45.0	45.0	58.0	58.0	35.1	2.3	0.056929	127213	127332	1117-1123	1.000000	82.8