primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6278	7179	973-975	1.000000	78.5
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	6278	7179	984-986	1.000000	78.0
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CACACCGGATCATGTCCTTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.3	58.1	9.5	16.9	0.419449	6278	7179	993-995	1.000000	76.9
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CACCGGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.6	58.1	9.5	16.9	0.477684	6278	7179	991-993	1.000000	76.4
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CCAAGAGCACCTTCATTCCT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.5	58.1	0.0	16.9	0.579996	6278	7179	1044-1046	1.000000	75.6
trnG-UCC_p1	trnG-UCC	1	AGAGTCTGTTAGAGTATTTGAGA	ACATATACAGAAGCGCCTCG	23	20	34.8	50.0	54.0	57.6	0.0	2.3	7.438959	29914	30696	909-909	1.000000	40.1
trnG-UCC_p2	trnG-UCC	2	AGAGTCTGTTAGAGTATTTGAGA	GCCTCGACAAAAGGTCCATT	23	20	34.8	50.0	54.0	58.5	0.0	0.0	7.480305	29914	30696	895-895	1.000000	40.1
trnG-UCC_p3	trnG-UCC	3	AGAGTCTGTTAGAGTATTTGAGA	TATACAGAAGCGCCTCGACA	23	20	34.8	50.0	54.0	58.6	0.0	2.3	7.629505	29914	30696	906-906	1.000000	40.1
trnG-UCC_p4	trnG-UCC	4	AGAGTCTGTTAGAGTATTTGAGAA	ACATATACAGAAGCGCCTCG	24	20	33.3	50.0	54.8	57.6	0.0	2.3	7.668135	29914	30696	909-909	1.000000	40.1
trnG-UCC_p5	trnG-UCC	5	AGAGTCTGTTAGAGTATTTGAGAA	GCCTCGACAAAAGGTCCATT	24	20	33.3	50.0	54.8	58.5	0.0	0.0	7.709480	29914	30696	895-895	1.000000	40.1
trnG-UCC-psbD_p1	trnG-UCC-psbD	1	AATCAAACCGAAAGACCCCT	GAAACGGTCCCTACGTAACC	20	20	45.0	55.0	57.0	58.0	0.0	5.0	0.975609	30697	32087	1550-1552	1.000000	72.3
trnG-UCC-psbD_p2	trnG-UCC-psbD	2	ATCAAACCGAAAGACCCCTT	GAAACGGTCCCTACGTAACC	20	20	45.0	55.0	57.0	58.0	0.0	5.0	0.975609	30697	32087	1549-1551	1.000000	72.3
trnG-UCC-psbD_p3	trnG-UCC-psbD	3	CAATCAAACCGAAAGACCCC	GAAACGGTCCCTACGTAACC	20	20	50.0	55.0	57.0	58.0	0.0	5.0	1.013365	30697	32087	1551-1553	1.000000	72.0
trnG-UCC-psbD_p4	trnG-UCC-psbD	4	AATCAAACCGAAAGACCCCT	GGACCAGCCTACAAAAACGA	20	20	45.0	50.0	57.0	58.1	0.0	0.0	1.085014	30697	32087	1568-1570	1.000000	71.4
trnG-UCC-psbD_p5	trnG-UCC-psbD	5	ATCAAACCGAAAGACCCCTT	GGACCAGCCTACAAAAACGA	20	20	45.0	50.0	57.0	58.1	0.0	0.0	1.085014	30697	32087	1567-1569	1.000000	71.4
psaA-ycf3_p1	psaA-ycf3	1	AGAAATGACCCGGTTTAGCC	CGACCAAGCTGCTGAGTATT	20	20	50.0	50.0	57.9	58.0	0.0	1.4	0.140013	41062	41786	921-922	1.000000	79.1
psaA-ycf3_p2	psaA-ycf3	2	AGAAATGACCCGGTTTAGCC	ACCAAGCTGCTGAGTATTGG	20	20	50.0	50.0	57.9	57.9	0.0	12.9	0.244078	41062	41786	919-920	1.000000	78.2
psaA-ycf3_p3	psaA-ycf3	3	CCCATGCCTCGAATGAAGTT	CGACCAAGCTGCTGAGTATT	20	20	50.0	50.0	58.2	58.0	0.0	1.4	0.260556	41062	41786	903-904	1.000000	78.1
psaA-ycf3_p4	psaA-ycf3	4	GTTTAGCCCATGCCTCGAAT	CGACCAAGCTGCTGAGTATT	20	20	50.0	50.0	58.3	58.0	0.0	1.4	0.335398	41062	41786	909-910	1.000000	77.5
psaA-ycf3_p5	psaA-ycf3	5	TTTAGCCCATGCCTCGAATG	CGACCAAGCTGCTGAGTATT	20	20	50.0	50.0	58.3	58.0	0.0	1.4	0.336597	41062	41786	908-909	1.000000	77.5
ycf3_p1	ycf3	1	TGATGGGGCCAACAAACAAT	TCCCGCGAGAAGTAATGAGA	20	20	45.0	50.0	58.3	58.2	4.4	8.2	0.514002	41787	43738	2088-2088	1.000000	75.9
ycf3_p2	ycf3	2	TGATGGGGCCAACAAACAAT	GGATCATTTTCCCGCGAGAA	20	20	45.0	50.0	58.3	58.3	4.4	8.2	0.609243	41787	43738	2097-2097	1.000000	75.2
ycf3_p3	ycf3	3	TGATGGGGCCAACAAACAAT	ATCATTTTCCCGCGAGAAGT	20	20	45.0	45.0	58.3	57.6	4.4	8.2	0.676190	41787	43738	2095-2095	1.000000	74.6
ycf3_p4	ycf3	4	ATTTGACTGATGGGGCCAAC	TCCCGCGAGAAGTAATGAGA	20	20	50.0	50.0	58.4	58.2	4.4	8.2	0.680559	41787	43738	2095-2095	1.000000	74.6
ycf3_p5	ycf3	5	ATTTGACTGATGGGGCCAAC	GGATCATTTTCCCGCGAGAA	20	20	50.0	50.0	58.4	58.3	4.4	8.2	0.775801	41787	43738	2104-2104	1.000000	73.8
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GTCCTTTGCTAATCGGAGCT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.7	57.8	0.0	0.0	0.514813	49619	49964	491-492	1.000000	75.9
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CGGTCCTTTGCTAATCGGAG	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	58.4	57.8	0.0	0.0	0.625364	49619	49964	493-494	1.000000	75.0
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CTCCGGTCCTTTGCTAATCG	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	58.4	57.8	0.0	0.0	0.625364	49619	49964	496-497	1.000000	75.0
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GTCCTTTGCTAATCGGAGCT	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	57.7	57.5	0.0	0.0	0.858585	49619	49964	490-491	1.000000	73.1
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GGTCCTTTGCTAATCGGAGC	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	58.7	57.8	3.4	0.0	0.896802	49619	49964	492-493	1.000000	72.8
trnV-UAC_p1	trnV-UAC	1	TGTTTTCAGCTCAATCTTACCG	ACCAACCAATATGATCTTGGATCA	22	24	40.9	37.5	57.0	58.0	0.0	15.5	7.026224	49965	50613	854-854	1.000000	40.1
trnV-UAC_p2	trnV-UAC	2	TGTTTTCAGCTCAATCTTACCG	ACTTAGAAGCAATACCAACCA	22	21	40.9	38.1	57.0	54.8	0.0	0.0	7.178071	49965	50613	867-867	1.000000	40.1
trnV-UAC_p3	trnV-UAC	3	GTTTTCAGCTCAATCTTACCGA	ACCAACCAATATGATCTTGGATCA	22	24	40.9	37.5	56.7	58.0	0.0	15.5	7.288204	49965	50613	853-853	1.000000	40.1
trnV-UAC_p4	trnV-UAC	4	TTGTTTTCAGCTCAATCTTACCG	ACCAACCAATATGATCTTGGATCA	23	24	39.1	37.5	57.6	58.0	0.0	15.5	7.374093	49965	50613	855-855	1.000000	40.1
trnV-UAC_p5	trnV-UAC	5	TGTTTTCAGCTCAATCTTACCG	ACTTAGAAGCAATACCAACCAA	22	22	40.9	36.4	57.0	55.6	0.0	0.0	7.378090	49965	50613	867-867	1.000000	40.1
clpP_p1	clpP	1	ATTGACGCTAAATCCAGGCT	GGCTTTGTTACGTTTCCACA	20	20	45.0	45.0	57.3	56.9	0.0	0.0	1.857009	68403	70412	2095-2096	1.000000	65.2
clpP_p2	clpP	2	ACTCGGCAGGGTTAATTTCA	GGCTTTGTTACGTTTCCACA	20	20	45.0	45.0	57.1	56.9	0.0	0.0	2.010467	68403	70412	2123-2124	1.000000	64.0
clpP_p3	clpP	3	ATTGACGCTAAATCCAGGCT	AGGCTTTGTTACGTTTCCACA	20	21	45.0	42.9	57.3	58.4	0.0	0.0	2.071837	68403	70412	2096-2097	1.000000	63.5
clpP_p4	clpP	4	AATTGACGCTAAATCCAGGCT	GGCTTTGTTACGTTTCCACA	21	20	42.9	45.0	58.0	56.9	0.0	0.0	2.144122	68403	70412	2096-2097	1.000000	62.9
clpP_p5	clpP	5	ATTGACGCTAAATCCAGGCT	AGGCTTTGTTACGTTTCCAC	20	20	45.0	45.0	57.3	56.6	0.0	0.0	2.152460	68403	70412	2096-2097	1.000000	62.8
ycf2_p1	ycf2	1	GAGATGTCAAAGGGGCTTCT	TGATAAATACTGATAACTCTCGGA	20	24	50.0	33.3	57.2	54.7	0.0	0.0	8.086509	91422	91571	195-195	1.000000	46.2
ycf2_p2	ycf2	2	AGAGATGTCAAAGGGGCTTC	TGATAAATACTGATAACTCTCGGA	20	24	50.0	33.3	57.2	54.7	0.0	0.0	8.086509	91422	91571	196-196	1.000000	46.1
ycf2_p3	ycf2	3	GAAGAGATGTCAAAGGGGCT	TGATAAATACTGATAACTCTCGGA	20	24	50.0	33.3	57.2	54.7	0.0	0.0	8.086509	91422	91571	198-198	1.000000	46.1
ycf2_p4	ycf2	4	TGAAGAGATGTCAAAGGGGC	TGATAAATACTGATAACTCTCGGA	20	24	50.0	33.3	57.5	54.7	0.0	0.0	7.786230	91422	91571	199-199	1.000000	46.0
ycf2_p5	ycf2	5	TGAAGAGATGTCAAAGGGGC	TTGATAAATACTGATAACTCTCGGA	20	25	50.0	32.0	57.5	55.4	0.0	0.0	8.077698	91422	91571	200-200	1.000000	46.0
ycf2-trnL_p1	ycf2-trnL	1	CCGGAAAGATATGTGGCCAT	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	57.7	58.0	9.7	15.9	0.284562	91747	92165	510-994	1.000000	83.3
ycf2-trnL_p2	ycf2-trnL	2	CCGGAAAGATATGTGGCCAT	TGAAATGGTAGACACGCGAG	20	20	50.0	50.0	57.7	58.0	9.7	15.9	0.285933	91747	92165	512-996	1.000000	83.3
ycf2-trnL_p3	ycf2-trnL	3	CCCATTCCTTAGCCGGAAAG	AAATGGTAGACACGCGAGAC	20	20	55.0	50.0	58.3	58.0	5.0	15.9	0.327493	91747	92165	522-1006	1.000000	82.9
ycf2-trnL_p4	ycf2-trnL	4	CCCATTCCTTAGCCGGAAAG	TGAAATGGTAGACACGCGAG	20	20	55.0	50.0	58.3	58.0	5.0	15.9	0.328863	91747	92165	524-1008	1.000000	82.9
ycf2-trnL_p5	ycf2-trnL	5	CCGGAAAGATATGTGGCCAT	GCCTTGGTGGTGAAATGGTA	20	20	50.0	50.0	57.7	58.1	9.7	0.0	0.358860	91747	92165	522-1006	1.000000	82.7
ndhF-rpl32_p1	ndhF-rpl32	1	GTTGCCGTCGGAAAAAGAAG	CGCCCAATACCCTTTCCTTT	20	20	50.0	50.0	58.0	58.2	17.5	0.0	0.185086	109898	110979	1261-1261	1.000000	78.6
ndhF-rpl32_p2	ndhF-rpl32	2	TGCCGTCGGAAAAAGAAGAA	CGCCCAATACCCTTTCCTTT	20	20	45.0	50.0	58.0	58.2	17.5	0.0	0.196375	109898	110979	1259-1259	1.000000	78.5
ndhF-rpl32_p3	ndhF-rpl32	3	TTGCCGTCGGAAAAAGAAGA	CGCCCAATACCCTTTCCTTT	20	20	45.0	50.0	58.0	58.2	17.5	0.0	0.196375	109898	110979	1260-1260	1.000000	78.5
ndhF-rpl32_p4	ndhF-rpl32	4	GTTGCCGTCGGAAAAAGAAG	CTTTTAACGCCGCCCAATAC	20	20	50.0	50.0	58.0	57.8	17.5	0.0	0.215318	109898	110979	1271-1271	1.000000	78.3
ndhF-rpl32_p5	ndhF-rpl32	5	TGCCGTCGGAAAAAGAAGAA	CTTTTAACGCCGCCCAATAC	20	20	45.0	50.0	58.0	57.8	17.5	0.0	0.226607	109898	110979	1269-1269	1.000000	78.3
rpl32-trnL_p1	rpl32-trnL	1	AAAGGAAAGGGTATTGGGCG	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.160502	111145	112032	1021-1021	1.000000	78.8
rpl32-trnL_p2	rpl32-trnL	2	AAAGGAAAGGGTATTGGGCG	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.161872	111145	112032	1022-1022	1.000000	78.8
rpl32-trnL_p3	rpl32-trnL	3	GTATTGGGCGGCGTTAAAAG	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	57.8	58.0	9.4	0.0	0.190734	111145	112032	1011-1011	1.000000	78.6
rpl32-trnL_p4	rpl32-trnL	4	GTATTGGGCGGCGTTAAAAG	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	57.8	58.0	9.4	0.0	0.192105	111145	112032	1012-1012	1.000000	78.5
rpl32-trnL_p5	rpl32-trnL	5	AAAGGAAAGGGTATTGGGCG	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.276949	111145	112032	1031-1031	1.000000	77.9
ycf1_p1	ycf1	1	GACGGTCCATTATCCTGAAGA	CGCAGAATCGCTCTACAACT	21	20	47.6	50.0	56.9	58.1	0.0	0.0	2.183071	123494	123613	296-296	1.000000	63.1
ycf1_p2	ycf1	2	AGACGGTCCATTATCCTGAAG	CGCAGAATCGCTCTACAACT	21	20	47.6	50.0	56.9	58.1	4.1	0.0	2.191213	123494	123613	297-297	1.000000	63.0
ycf1_p3	ycf1	3	AGACGGTCCATTATCCTGAAGA	CGCAGAATCGCTCTACAACT	22	20	45.5	50.0	58.4	58.1	4.1	0.0	2.442129	123494	123613	297-297	1.000000	61.0
ycf1_p4	ycf1	4	ACGGTCCATTATCCTGAAGA	CGCAGAATCGCTCTACAACT	20	20	45.0	50.0	55.6	58.1	0.0	0.0	2.448497	123494	123613	295-295	1.000000	61.0
ycf1_p5	ycf1	5	AGACGGTCCATTATCCTGAA	CGCAGAATCGCTCTACAACT	20	20	45.0	50.0	55.6	58.1	4.1	0.0	2.448497	123494	123613	297-297	1.000000	61.0
trnL-ycf2_p1	trnL-ycf2	1	AAATGGTAGACACGCGAGAC	CCGGAAAGATATGTGGCCAT	20	20	50.0	50.0	58.0	57.7	15.9	9.7	0.284562	141629	142047	510-994	1.000000	83.3
trnL-ycf2_p2	trnL-ycf2	2	TGAAATGGTAGACACGCGAG	CCGGAAAGATATGTGGCCAT	20	20	50.0	50.0	58.0	57.7	15.9	9.7	0.285933	141629	142047	512-996	1.000000	83.3
trnL-ycf2_p3	trnL-ycf2	3	AAATGGTAGACACGCGAGAC	CCCATTCCTTAGCCGGAAAG	20	20	50.0	55.0	58.0	58.3	15.9	5.0	0.327493	141629	142047	522-1006	1.000000	82.9
trnL-ycf2_p4	trnL-ycf2	4	TGAAATGGTAGACACGCGAG	CCCATTCCTTAGCCGGAAAG	20	20	50.0	55.0	58.0	58.3	15.9	5.0	0.328863	141629	142047	524-1008	1.000000	82.9
trnL-ycf2_p5	trnL-ycf2	5	GCCTTGGTGGTGAAATGGTA	CCGGAAAGATATGTGGCCAT	20	20	50.0	50.0	58.1	57.7	0.0	9.7	0.358860	141629	142047	522-1006	1.000000	82.7