primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	AGAGAAGCGGCCATAAACTG	AAAAGATGCACGTTGGGTCT	20	20	50.0	45.0	58.0	58.0	2.2	0.0	0.048582	54544	55145	1224-1225	1.000000	84.2
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CGCACTAAAGAAGGAGCCAT	AAAAGATGCACGTTGGGTCT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.048582	54544	55145	1381-1382	1.000000	83.7
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CGTGGTACCAGTCCATAACG	AAAAGATGCACGTTGGGTCT	20	20	55.0	45.0	58.1	58.0	16.9	0.0	0.101471	54544	55145	1306-1307	1.000000	83.5
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TGGTTCAGGCATTTGCTCAT	AAAAGATGCACGTTGGGTCT	20	20	45.0	45.0	58.1	58.0	2.1	0.0	0.091256	54544	55145	1406-1407	1.000000	83.3
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	AGAGAAGCGGCCATAAACTG	GCACAAGGGCTATAGCTCAG	20	20	50.0	55.0	58.0	58.1	2.2	26.2	0.153516	54544	55145	1815-1816	1.000000	81.9
petB-petD_p1	petB-petD	1	TAAGCAAGGCATTTCCGGTC	GTTCAAGTCACACACTCCCA	20	20	50.0	50.0	58.5	57.7	0.0	0.0	0.866133	80683	80873	240-247	1.000000	81.0
petB-petD_p2	petB-petD	2	TAAGCAAGGCATTTCCGGTC	AAGTCATATATCTGCGCGGC	20	20	50.0	50.0	58.5	58.3	0.0	12.6	0.825756	80683	80873	270-277	1.000000	80.4
petB-petD_p3	petB-petD	3	CAAGGCATTTCCGGTCCTTT	GTTCAAGTCACACACTCCCA	20	20	50.0	50.0	58.7	57.7	0.0	0.0	1.068771	80683	80873	236-243	1.000000	79.5
petB-petD_p4	petB-petD	4	CAAGGCATTTCCGGTCCTTT	AAGTCATATATCTGCGCGGC	20	20	50.0	50.0	58.7	58.3	0.0	12.6	1.028393	80683	80873	266-273	1.000000	78.9
petB-petD_p5	petB-petD	5	GTAAGCAAGGCATTTCCGGT	AAGTCATATATCTGCGCGGC	20	20	50.0	50.0	58.8	58.3	0.0	12.6	1.111293	80683	80873	271-278	1.000000	78.1
ndhF_p1	ndhF	1	TGTTTCGATGCAACAACCAA	TCAAAAGAGATCAAATTGGCGA	20	22	40.0	36.4	56.8	56.6	0.0	0.0	4.616013	115038	117275	2827-2827	0.500000	23.1
ndhF_p2	ndhF	2	ATGTTTCGATGCAACAACCA	TCAAAAGAGATCAAATTGGCGA	20	22	40.0	36.4	56.5	56.6	5.3	0.0	4.843417	115038	117275	2828-2828	0.500000	21.3
ndhF_p3	ndhF	3	TGTTTCGATGCAACAACCAA	TCAAAAGAGATCAAATTGGCGAA	20	23	40.0	34.8	56.8	57.3	0.0	0.0	4.939299	115038	117275	2827-2827	0.500000	20.5
ndhF_p4	ndhF	4	TGTTTCGATGCAACAACCAA	TTCAAAAGAGATCAAATTGGCGA	20	23	40.0	34.8	56.8	57.3	0.0	0.0	4.939299	115038	117275	2828-2828	0.500000	20.5
ndhF_p5	ndhF	5	TGTTTCGATGCAACAACCAA	TGGCGAATAAGAAACAAAACTCA	20	23	40.0	34.8	56.8	57.2	0.0	0.0	4.994827	115038	117275	2811-2811	0.500000	20.0
ndhF-rpl32_p1	ndhF-rpl32	1	AAAACGCTAGGAAAAGCCCA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.018124	117276	118186	1661-1661	0.500000	59.9
ndhF-rpl32_p2	ndhF-rpl32	2	AAAACGCTAGGAAAAGCCCA	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.019495	117276	118186	1662-1662	0.500000	59.8
ndhF-rpl32_p3	ndhF-rpl32	3	AAAACGCTAGGAAAAGCCCA	AGCCAATTCCAAAATGGGGT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.053601	117276	118186	1485-1485	0.500000	59.6
ndhF-rpl32_p4	ndhF-rpl32	4	TAGGGCGTGTGAACCAAAAA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.062062	117276	118186	2032-2032	0.500000	59.5
ndhF-rpl32_p5	ndhF-rpl32	5	TAGGGCGTGTGAACCAAAAA	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.063433	117276	118186	2033-2033	0.500000	59.5
ccsA_p1	ccsA	1	GTGAAATCGGTAGACACGCT	GGTATGAGCGGTTTTGTTGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.030642	118947	119918	1763-1763	0.500000	59.8
ccsA_p2	ccsA	2	TGAAATCGGTAGACACGCTG	GGTATGAGCGGTTTTGTTGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032012	118947	119918	1762-1762	0.500000	59.7
ccsA_p3	ccsA	3	ACCCCATTTTGGAATTGGCT	GGTATGAGCGGTTTTGTTGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.066119	118947	119918	1939-1939	0.500000	59.5
ccsA_p4	ccsA	4	GTGAAATCGGTAGACACGCT	GGTGTTTGTGAGAACCCCTT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.075196	118947	119918	1280-1280	0.500000	59.4
ccsA_p5	ccsA	5	TGAAATCGGTAGACACGCTG	GGTGTTTGTGAGAACCCCTT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.076566	118947	119918	1279-1279	0.500000	59.4
ndhD_p1	ndhD	1	AATGAGGCCTGGGGATCATA	TCTGCTCCAAGAACAGAGGA	20	20	50.0	50.0	57.9	58.0	12.0	0.0	0.153991	120213	121718	2270-2270	0.500000	58.8
ndhD_p2	ndhD	2	TAATGAGGCCTGGGGATCAT	TCTGCTCCAAGAACAGAGGA	20	20	50.0	50.0	57.9	58.0	12.0	0.0	0.153991	120213	121718	2271-2271	0.500000	58.8
ndhD_p3	ndhD	3	TGAGGCCTGGGGATCATATT	TCTGCTCCAAGAACAGAGGA	20	20	50.0	50.0	57.9	58.0	12.0	0.0	0.153991	120213	121718	2268-2268	0.500000	58.8
ndhD_p4	ndhD	4	AATGAGGCCTGGGGATCATA	AAACAACTCGAAGCATGGGT	20	20	50.0	45.0	57.9	58.0	12.0	0.0	0.157278	120213	121718	2176-2176	0.500000	58.7
ndhD_p5	ndhD	5	TGAGGCCTGGGGATCATATT	AAACAACTCGAAGCATGGGT	20	20	50.0	45.0	57.9	58.0	12.0	0.0	0.157278	120213	121718	2174-2174	0.500000	58.7
ndhD-psaC_p1	ndhD-psaC	1	TTTCTTTCCACCCCACATGG	TCTGCTCCAAGAACAGAGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.023699	121719	121834	728-728	0.500000	59.8
ndhD-psaC_p2	ndhD-psaC	2	TTTCTTTCCACCCCACATGG	AAACAACTCGAAGCATGGGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.026986	121719	121834	634-634	0.500000	59.8
ndhD-psaC_p3	ndhD-psaC	3	TAATCGGCGATTTGACAGCA	TCTGCTCCAAGAACAGAGGA	20	20	45.0	50.0	58.0	58.0	36.3	0.0	0.032594	121719	121834	933-933	0.500000	59.7
ndhD-psaC_p4	ndhD-psaC	4	TAATCGGCGATTTGACAGCA	AAACAACTCGAAGCATGGGT	20	20	45.0	45.0	58.0	58.0	36.3	0.0	0.035881	121719	121834	839-839	0.500000	59.7
ndhD-psaC_p5	ndhD-psaC	5	TTTCTTTCCACCCCACATGG	GGCATGAAACAACTCGAAGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.039139	121719	121834	640-640	0.500000	59.7
psaC_p1	psaC	1	TTTCTTTCCACCCCACATGG	ACCGGCTATTGTTTCGTCAA	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.181094	121835	122080	1192-1192	0.500000	58.6
psaC_p2	psaC	2	CCCGTCAGTAAAATAGGGCC	ACCGGCTATTGTTTCGTCAA	20	20	55.0	45.0	58.0	57.8	0.0	0.0	0.209679	121835	122080	996-996	0.500000	58.3
psaC_p3	psaC	3	TTTCTTTCCACCCCACATGG	GCTATTGGACCGGCTATTGT	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.264360	121835	122080	1200-1200	0.500000	57.9
psaC_p4	psaC	4	TTTCTTTCCACCCCACATGG	GACCGGCTATTGTTTCGTCA	20	20	50.0	50.0	58.0	58.3	0.0	0.0	0.291049	121835	122080	1193-1193	0.500000	57.7
psaC_p5	psaC	5	CCCGTCAGTAAAATAGGGCC	GCTATTGGACCGGCTATTGT	20	20	55.0	50.0	58.0	57.7	0.0	0.0	0.292945	121835	122080	1004-1004	0.500000	57.7
ndhG-ndhI_p1	ndhG-ndhI	1	CAAGAACCAGTCCCAACGAA	AATCAAATTGCTTTGGGCCG	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.135886	123425	123850	631-631	0.500000	58.9
ndhG-ndhI_p2	ndhG-ndhI	2	TCCCAACGAAAAGGCAGAAA	AATCAAATTGCTTTGGGCCG	20	20	45.0	45.0	57.9	57.9	0.0	0.0	0.157730	123425	123850	621-621	0.500000	58.7
ndhG-ndhI_p3	ndhG-ndhI	3	CCCAACGGTCCAAAGATGAA	AATCAAATTGCTTTGGGCCG	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.193393	123425	123850	789-789	0.500000	58.5
ndhG-ndhI_p4	ndhG-ndhI	4	TTGCACCTATCAAAGCAGCT	AATCAAATTGCTTTGGGCCG	20	20	45.0	45.0	57.8	57.9	0.0	0.0	0.302702	123425	123850	1012-1012	0.500000	57.6
ndhG-ndhI_p5	ndhG-ndhI	5	CAAGAACCAGTCCCAACGAA	TTTGGGCCGTTTACCAATGT	20	20	50.0	45.0	58.0	58.3	0.0	0.0	0.332334	123425	123850	620-620	0.500000	57.3
ndhI_p1	ndhI	1	CAAGAACCAGTCCCAACGAA	GAATTTGTGGCGTCAACCTG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.088684	123851	124336	1598-1598	0.500000	59.3
ndhI_p2	ndhI	2	TCCCAACGAAAAGGCAGAAA	GAATTTGTGGCGTCAACCTG	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.110527	123851	124336	1588-1588	0.500000	59.1
ndhI_p3	ndhI	3	CAAGAACCAGTCCCAACGAA	ACAGTTCTTTACTTGGGCGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.145997	123851	124336	1402-1402	0.500000	58.8
ndhI_p4	ndhI	4	CAAGAACCAGTCCCAACGAA	CAGTTCTTTACTTGGGCGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.145997	123851	124336	1401-1401	0.500000	58.8
ndhI_p5	ndhI	5	CCCAACGGTCCAAAGATGAA	GAATTTGTGGCGTCAACCTG	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.146190	123851	124336	1756-1756	0.500000	58.8
ndhA_p1	ndhA	1	CCTTGCGGCTCGTACTATTT	GTGAGAGTTGAAGCCCCAAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.082811	124418	126860	2786-2786	0.500000	59.3
ndhA_p2	ndhA	2	CCTTGCGGCTCGTACTATTT	AAAAAGCCGTCTCCGACTTT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.100895	124418	126860	2831-2831	0.500000	59.2
ndhA_p3	ndhA	3	CCTTGCGGCTCGTACTATTT	CAGGCTCTGGAAGGAATTCC	20	20	50.0	55.0	58.1	58.0	0.0	17.3	0.113956	124418	126860	2939-2939	0.500000	59.1
ndhA_p4	ndhA	4	TACCTTGCGGCTCGTACTAT	GTGAGAGTTGAAGCCCCAAA	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.334800	124418	126860	2788-2788	0.500000	57.3
ndhA_p5	ndhA	5	CCTTGCGGCTCGTACTATTT	TGAGAGTTGAAGCCCCAAAG	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.340800	124418	126860	2785-2785	0.500000	57.3
ndhH_p1	ndhH	1	TCAGGTCCAATACGCTGTTG	GAAAACCCGGGATCGATTGA	20	20	50.0	50.0	57.9	58.0	0.0	32.0	0.125987	126862	128043	1699-1699	0.500000	59.0
ndhH_p2	ndhH	2	TCAGGTCCAATACGCTGTTG	AGAAAACCCGGGATCGATTG	20	20	50.0	50.0	57.9	58.0	0.0	32.0	0.131693	126862	128043	1700-1700	0.500000	58.9
ndhH_p3	ndhH	3	TCAGGTCCAATACGCTGTTG	CTGCGAAGACAAATCGATGC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.176039	126862	128043	2099-2099	0.500000	58.6
ndhH_p4	ndhH	4	TCAGGTCCAATACGCTGTTG	CGGGAGCCACAAACTCATTA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.201256	126862	128043	1501-1501	0.500000	58.4
ndhH_p5	ndhH	5	TCAGGTCCAATACGCTGTTG	AAGAAGAAAACCCGGGATCG	20	20	50.0	50.0	57.9	57.9	0.0	32.0	0.202731	126862	128043	1704-1704	0.500000	58.4
rps15_p1	rps15	1	ACTCCCTCAACCCGTTCTAA	GCAGCCGTTTCAGTATGGTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.038349	128147	128413	1437-1437	0.500000	59.7
rps15_p2	rps15	2	CAATGCCTACTCCCTCAACC	GCAGCCGTTTCAGTATGGTA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.062665	128147	128413	1445-1445	0.500000	59.5
rps15_p3	rps15	3	CCAACAATGCCTACTCCCTC	GCAGCCGTTTCAGTATGGTA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.062665	128147	128413	1449-1449	0.500000	59.5
rps15_p4	rps15	4	TCCTAACTGTTCCGGTCCAT	GCAGCCGTTTCAGTATGGTA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.071557	128147	128413	1090-1090	0.500000	59.4
rps15_p5	rps15	5	AACACCATGCATTGATGGGT	GCAGCCGTTTCAGTATGGTA	20	20	45.0	50.0	58.1	58.0	8.9	0.0	0.077165	128147	128413	895-895	0.500000	59.4
rps15-ycf1_p1	rps15-ycf1	1	TCAATCGATCCCGGGTTTTC	GCAGCCGTTTCAGTATGGTA	20	20	50.0	50.0	58.0	58.0	22.9	0.0	0.051222	128414	128807	518-518	0.500000	59.6
rps15-ycf1_p2	rps15-ycf1	2	CAATCGATCCCGGGTTTTCT	GCAGCCGTTTCAGTATGGTA	20	20	50.0	50.0	58.0	58.0	22.9	0.0	0.056929	128414	128807	517-517	0.500000	59.5
rps15-ycf1_p3	rps15-ycf1	3	TCCTAACTGTTCCGGTCCAT	GCAGCCGTTTCAGTATGGTA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.071557	128414	128807	1090-1090	0.500000	59.4
rps15-ycf1_p4	rps15-ycf1	4	AACACCATGCATTGATGGGT	GCAGCCGTTTCAGTATGGTA	20	20	45.0	50.0	58.1	58.0	8.9	0.0	0.077165	128414	128807	895-895	0.500000	59.4
rps15-ycf1_p5	rps15-ycf1	5	CTTGTAGCTGGGGCATTCAT	GCAGCCGTTTCAGTATGGTA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.081512	128414	128807	840-840	0.500000	59.3
ycf1_p1	ycf1	1	TACCATACTGAAACGGCTGC	ATCGTTCCATAGCGAATCGG	20	20	50.0	50.0	58.0	57.9	0.0	4.4	0.084535	129437	129556	995-995	0.500000	59.3
ycf1_p2	ycf1	2	ACTGAAACGGCTGCCATTAT	ATCGTTCCATAGCGAATCGG	20	20	45.0	50.0	57.9	57.9	20.3	4.4	0.194763	129437	129556	989-989	0.500000	58.4
ycf1_p3	ycf1	3	ATACTGAAACGGCTGCCATT	ATCGTTCCATAGCGAATCGG	20	20	45.0	50.0	57.9	57.9	20.3	4.4	0.194763	129437	129556	991-991	0.500000	58.4
ycf1_p4	ycf1	4	TACCATACTGAAACGGCTGC	GTTCCATAGCGAATCGGGAA	20	20	50.0	50.0	58.0	57.8	0.0	4.4	0.252842	129437	129556	992-992	0.500000	58.0
ycf1_p5	ycf1	5	GAAACGGCTGCCATTATTGG	ATCGTTCCATAGCGAATCGG	20	20	50.0	50.0	57.8	57.9	20.3	4.4	0.274318	129437	129556	986-986	0.500000	57.8