primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps12_p1	rps12	1	TACGGTTCCGTAGAGTGACA	TCTGCGATACTTTTACGTGACA	20	22	50.0	40.9	57.8	57.8	18.3	0.0	2.346936	371	1101	834-834	1.000000	61.2
rps12_p2	rps12	2	TACGGTTCCGTAGAGTGACA	TGGATCAGGCTTTCCTACTT	20	20	50.0	45.0	57.8	55.8	18.3	13.0	2.361337	371	1101	859-859	1.000000	61.1
rps12_p3	rps12	3	TACGGTTCCGTAGAGTGACA	TTGGATCAGGCTTTCCTACT	20	20	50.0	45.0	57.8	55.8	18.3	13.0	2.361337	371	1101	860-860	1.000000	61.1
rps12_p4	rps12	4	ACGAGGAGCCGTATGAAATG	TCTGCGATACTTTTACGTGACA	20	22	50.0	40.9	57.8	57.8	0.0	0.0	2.388527	371	1101	865-865	1.000000	60.9
rps12_p5	rps12	5	ATTGAACGAGGAGCCGTATG	TCTGCGATACTTTTACGTGACA	20	22	50.0	40.9	57.8	57.8	0.0	0.0	2.388527	371	1101	870-870	1.000000	60.9
ycf66_p1	ycf66	1	TTTTTCAGCAGATGCAACGG	AAACAAACCCCAGTCGATCC	20	20	45.0	50.0	57.6	58.1	7.7	0.0	0.514991	4584	5493	1086-1087	1.000000	76.0
ycf66_p2	ycf66	2	TTTTTCAGCAGATGCAACGG	GGAAATTGAATCCCGTGACG	20	20	45.0	50.0	57.6	57.2	7.7	0.0	1.245714	4584	5493	1008-1009	1.000000	70.1
ycf66_p3	ycf66	3	TTTTTCAGCAGATGCAACGG	TGCCTACCAGTATTTTTGCGA	20	21	45.0	42.9	57.6	57.9	7.7	0.0	1.481364	4584	5493	1044-1045	1.000000	68.2
ycf66_p4	ycf66	4	TTTTTCAGCAGATGCAACGG	TAAACAAACCCCAGTCGATCC	20	21	45.0	47.6	57.6	57.9	7.7	0.0	1.482751	4584	5493	1087-1088	1.000000	68.2
ycf66_p5	ycf66	5	TTTTCAGCAGATGCAACGGG	AAACAAACCCCAGTCGATCC	20	20	50.0	50.0	59.4	58.1	7.7	0.0	1.496794	4584	5493	1085-1086	1.000000	68.1
rpoB_p1	rpoB	1	CAGCATCGAATGAGAGGGAG	TGATTGGGATAGCGTCTTCG	20	20	55.0	50.0	57.8	57.5	0.0	0.0	0.660933	8045	8164	234-234	1.000000	75.1
rpoB_p2	rpoB	2	CTCCAGCATCGAATGAGAGG	TGATTGGGATAGCGTCTTCG	20	20	55.0	50.0	57.8	57.5	0.0	0.0	0.660933	8045	8164	237-237	1.000000	75.1
rpoB_p3	rpoB	3	ACGCACGGATTTTATCACGT	TGATTGGGATAGCGTCTTCG	20	20	45.0	50.0	58.3	57.5	0.0	0.0	0.791858	8045	8164	212-212	1.000000	74.0
rpoB_p4	rpoB	4	GGAGGTACGCACGGATTTTA	TGATTGGGATAGCGTCTTCG	20	20	50.0	50.0	57.7	57.5	0.0	0.0	0.807463	8045	8164	218-218	1.000000	73.9
rpoB_p5	rpoB	5	TCGAATGAGAGGGAGGTACG	TGATTGGGATAGCGTCTTCG	20	20	55.0	50.0	58.3	57.5	0.0	0.0	0.832306	8045	8164	229-229	1.000000	73.7
rps2_p1	rps2	1	TCCCATGCATATGCGTATGT	CACCACCACTACCATTACCG	20	20	45.0	55.0	57.1	58.0	21.7	0.0	0.875728	16400	17110	854-854	1.000000	73.0
rps2_p2	rps2	2	TCCCATGCATATGCGTATGT	ATCACTCACCACCACTACCA	20	20	45.0	50.0	57.1	58.0	21.7	0.0	0.881362	16400	17110	860-860	1.000000	72.9
rps2_p3	rps2	3	TCCCATGCATATGCGTATGT	TCACTCACCACCACTACCAT	20	20	45.0	50.0	57.1	58.0	21.7	0.0	0.881362	16400	17110	859-859	1.000000	72.9
rps2_p4	rps2	4	CCCATGCATATGCGTATGTG	CACCACCACTACCATTACCG	20	20	50.0	55.0	57.1	58.0	21.7	0.0	0.924501	16400	17110	853-853	1.000000	72.6
rps2_p5	rps2	5	CCCATGCATATGCGTATGTG	TCACTCACCACCACTACCAT	20	20	50.0	50.0	57.1	58.0	21.7	0.0	0.930135	16400	17110	858-858	1.000000	72.6
atpI_p1	atpI	1	ACATTTCCGGAGAGAGCAAC	GCCATCGTCGACCCAATAAT	20	20	50.0	50.0	57.9	58.1	3.3	10.6	0.221652	17330	18076	797-797	1.000000	78.2
atpI_p2	atpI	2	ACATTTCCGGAGAGAGCAAC	CAATGAAAGCCATCGTCGAC	20	20	50.0	50.0	57.9	57.6	3.3	5.2	0.542794	17330	18076	805-805	1.000000	75.7
atpI_p3	atpI	3	ACATTTCCGGAGAGAGCAAC	TGTGAATCGATTCGACGACG	20	20	50.0	50.0	57.9	58.5	3.3	28.1	0.569748	17330	18076	832-832	1.000000	75.4
atpI_p4	atpI	4	AAGGAATGTTGGCGAGGAAT	GCCATCGTCGACCCAATAAT	20	20	45.0	50.0	57.5	58.1	0.0	10.6	0.635505	17330	18076	838-838	1.000000	74.9
atpI_p5	atpI	5	AGGAATGTTGGCGAGGAATT	GCCATCGTCGACCCAATAAT	20	20	45.0	50.0	57.5	58.1	0.0	10.6	0.635505	17330	18076	837-837	1.000000	74.9
psaM-trnS-GCU_p1	psaM-trnS-GCU	1	ACTCCAATCTCGGATAGCCA	TAGTTGCCAGGAGAGAGAGG	20	20	50.0	55.0	57.9	57.8	0.0	0.0	0.239607	23011	23223	401-405	1.000000	78.9
psaM-trnS-GCU_p2	psaM-trnS-GCU	2	CGTTTTCGCAACAGGTGTTT	TAGTTGCCAGGAGAGAGAGG	20	20	45.0	55.0	58.1	57.8	0.0	0.0	0.248662	23011	23223	360-364	1.000000	78.9
psaM-trnS-GCU_p3	psaM-trnS-GCU	3	ACTCCAATCTCGGATAGCCA	GTGCTTTAGTTGCCAGGAGA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.273108	23011	23223	407-411	1.000000	78.6
psaM-trnS-GCU_p4	psaM-trnS-GCU	4	CGTTTTCGCAACAGGTGTTT	GTGCTTTAGTTGCCAGGAGA	20	20	45.0	50.0	58.1	57.8	0.0	0.0	0.282164	23011	23223	366-370	1.000000	78.6
psaM-trnS-GCU_p5	psaM-trnS-GCU	5	TTTCGCAACAGGTGTTTTCG	TAGTTGCCAGGAGAGAGAGG	20	20	45.0	55.0	57.8	57.8	7.6	0.0	0.332928	23011	23223	357-361	1.000000	78.2
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	TTGACAGGGCGGTACTCTAA	ACTGTAAATCCGCTGGCAAT	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.201283	36710	36866	247-247	1.000000	78.7
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	TTGACAGGGCGGTACTCTAA	CTCGAGTGGTTAATGGGGAC	20	20	50.0	55.0	58.1	57.7	0.0	3.6	0.383637	36710	36866	269-269	1.000000	77.2
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	TTGACAGGGCGGTACTCTAA	ATGCTCGAGTGGTTAATGGG	20	20	50.0	50.0	58.1	57.7	0.0	15.1	0.404576	36710	36866	272-272	1.000000	77.1
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	TTGACAGGGCGGTACTCTAA	CTGTAAATCCGCTGGCAATG	20	20	50.0	50.0	58.1	57.5	0.0	0.0	0.552737	36710	36866	246-246	1.000000	75.9
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	CAACTTCCGTCTTGACAGGG	ACTGTAAATCCGCTGGCAAT	20	20	55.0	45.0	58.5	57.9	1.6	0.0	0.615844	36710	36866	258-258	1.000000	75.4
psbZ_p1	psbZ	1	CTCGAACAGTTTTACGGGGA	TCGAAACACTTGCTCTAAATGC	20	22	50.0	40.9	57.6	57.6	0.0	0.0	2.848394	41148	41336	394-394	1.000000	57.4
psbZ_p2	psbZ	2	TCTCGAACAGTTTTACGGGG	TCGAAACACTTGCTCTAAATGC	20	22	50.0	40.9	57.6	57.6	0.0	0.0	2.848394	41148	41336	395-395	1.000000	57.4
psbZ_p3	psbZ	3	CTCGAACAGTTTTACGGGGA	CGAAACACTTGCTCTAAATGCT	20	22	50.0	40.9	57.6	57.6	0.0	0.0	2.854380	41148	41336	393-393	1.000000	57.4
psbZ_p4	psbZ	4	TCTCGAACAGTTTTACGGGG	CGAAACACTTGCTCTAAATGCT	20	22	50.0	40.9	57.6	57.6	0.0	0.0	2.854380	41148	41336	394-394	1.000000	57.4
psbZ_p5	psbZ	5	CTCGAACAGTTTTACGGGGA	GGATGTAAACCAAGGAGGATGA	20	22	50.0	45.5	57.6	57.5	0.0	0.0	2.947601	41148	41336	355-355	1.000000	56.6
trnV-UAC-trnM-CAU_p1	trnV-UAC-trnM-CAU	1	TACCGCTGAGCTATAACCCT	GCGATACTCCAACCACTGAG	20	20	50.0	55.0	57.6	58.1	1.8	0.0	0.448800	53183	53370	232-238	1.000000	78.5
trnV-UAC-trnM-CAU_p2	trnV-UAC-trnM-CAU	2	ACAAGGAAACCGTGAATCGA	GCGATACTCCAACCACTGAG	20	20	45.0	55.0	57.5	58.1	10.3	0.0	0.608866	53183	53370	305-311	1.000000	76.7
trnV-UAC-trnM-CAU_p3	trnV-UAC-trnM-CAU	3	CGTGTAAACGAGGTGCTCTA	GCGATACTCCAACCACTGAG	20	20	50.0	55.0	57.4	58.1	0.0	0.0	0.696421	53183	53370	250-256	1.000000	76.3
trnV-UAC-trnM-CAU_p4	trnV-UAC-trnM-CAU	4	CACAAGGAAACCGTGAATCG	GCGATACTCCAACCACTGAG	20	20	50.0	55.0	57.4	58.1	10.3	0.0	0.666634	53183	53370	306-312	1.000000	76.2
trnV-UAC-trnM-CAU_p5	trnV-UAC-trnM-CAU	5	TCTCTGACAATCATTGGCGC	GCGATACTCCAACCACTGAG	20	20	50.0	55.0	58.6	58.1	0.0	0.0	0.699523	53183	53370	271-277	1.000000	76.2
trnR-CCG-accD_p1	trnR-CCG-accD	1	CCGAATCATCAGGTCAAGGG	CCTTTCGTCGCTTTTTCGAT	20	20	55.0	45.0	58.0	57.1	0.0	0.0	0.916038	57420	57627	327-328	1.000000	72.7
trnR-CCG-accD_p2	trnR-CCG-accD	2	TGGTAGCTCAGCGGATTAGA	CCTTTCGTCGCTTTTTCGAT	20	20	50.0	45.0	57.9	57.1	0.0	0.0	0.942445	57420	57627	357-358	1.000000	72.5
trnR-CCG-accD_p3	trnR-CCG-accD	3	CCTTTTCGGAGACTTCCCAG	CCTTTCGTCGCTTTTTCGAT	20	20	55.0	45.0	57.9	57.1	0.0	0.0	0.975684	57420	57627	389-390	1.000000	72.2
trnR-CCG-accD_p4	trnR-CCG-accD	4	TCCTTTTCGGAGACTTCCCA	CCTTTCGTCGCTTTTTCGAT	20	20	50.0	45.0	58.3	57.1	0.0	0.0	1.150535	57420	57627	390-391	1.000000	70.8
trnR-CCG-accD_p5	trnR-CCG-accD	5	CCGAATCATCAGGTCAAGGG	TAGCCTTTCGTCGCTTTTTC	20	20	55.0	45.0	58.0	56.8	0.0	0.0	1.285239	57420	57627	330-331	1.000000	69.7
rps18_p1	rps18	1	TGGGTGCACTATCCACAAAG	CCTCCCCCGGTTAATTTCTG	20	20	50.0	55.0	57.8	57.9	18.0	0.0	0.251609	65020	65247	355-355	1.000000	78.2
rps18_p2	rps18	2	TGGGTGCACTATCCACAAAG	CTCCCCCGGTTAATTTCTGG	20	20	50.0	55.0	57.8	57.9	18.0	0.0	0.251609	65020	65247	354-354	1.000000	78.2
rps18_p3	rps18	3	TACTGTGGTGGGTGCACTAT	CCTCCCCCGGTTAATTTCTG	20	20	50.0	55.0	58.3	57.9	18.0	0.0	0.401045	65020	65247	363-363	1.000000	77.0
rps18_p4	rps18	4	TACTGTGGTGGGTGCACTAT	CTCCCCCGGTTAATTTCTGG	20	20	50.0	55.0	58.3	57.9	18.0	0.0	0.401045	65020	65247	362-362	1.000000	77.0
rps18_p5	rps18	5	TTTTGTCGCTACTGTGGTGG	CTCCCCCGGTTAATTTCTGG	20	20	50.0	55.0	58.4	57.9	4.9	0.0	0.464108	65020	65247	371-371	1.000000	76.5
clpP-psbB_p1	clpP-psbB	1	AAAGGAACCTTCGGAACACC	GCACAGCAATTAAACGACCC	20	20	50.0	50.0	58.0	58.0	6.6	0.0	0.045755	68154	68513	456-456	1.000000	79.6
clpP-psbB_p2	clpP-psbB	2	GGAACCTTCGGAACACCAAT	GCACAGCAATTAAACGACCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.117174	68154	68513	453-453	1.000000	79.1
clpP-psbB_p3	clpP-psbB	3	AAAGGAACCTTCGGAACACC	GACGCGATACCAAGGTAGAC	20	20	50.0	55.0	58.0	57.9	6.6	15.9	0.139731	68154	68513	413-413	1.000000	78.9
clpP-psbB_p4	clpP-psbB	4	GACGAAAAGGAACCTTCGGA	GCACAGCAATTAAACGACCC	20	20	50.0	50.0	57.8	58.0	16.0	0.0	0.182139	68154	68513	461-461	1.000000	78.5
clpP-psbB_p5	clpP-psbB	5	AGACGAAAAGGAACCTTCGG	GCACAGCAATTAAACGACCC	20	20	50.0	50.0	57.8	58.0	16.0	0.0	0.188130	68154	68513	462-462	1.000000	78.5
psbB_p1	psbB	1	GCGGTTTCTTTCGATGCAAA	AGAAGAAACGATGATGATGCCT	20	22	45.0	40.9	58.0	57.9	0.0	0.0	2.193016	68514	70031	1667-1667	1.000000	62.5
psbB_p2	psbB	2	TAGCGGTTTCTTTCGATGCA	AGAAGAAACGATGATGATGCCT	20	22	45.0	40.9	57.9	57.9	0.0	0.0	2.235629	68514	70031	1669-1669	1.000000	62.2
psbB_p3	psbB	3	GTAGCGGTTTCTTTCGATGC	AGAAGAAACGATGATGATGCCT	20	22	50.0	40.9	57.6	57.9	0.0	0.0	2.585436	68514	70031	1670-1670	1.000000	59.4
psbB_p4	psbB	4	GCGGTTTCTTTCGATGCAAA	AGAAGAAACGATGATGATGCC	20	21	45.0	42.9	58.0	56.4	0.0	0.0	2.644300	68514	70031	1667-1667	1.000000	58.9
psbB_p5	psbB	5	GCGGTTTCTTTCGATGCAAA	GAAGAAACGATGATGATGCCT	20	21	45.0	42.9	58.0	56.4	0.0	0.0	2.644300	68514	70031	1666-1666	1.000000	58.9
rps3-rpl22_p1	rps3-rpl22	1	TTGCGAACCAGTATGAACGA	TCAACCTAGAGCTCAGGGAC	20	20	45.0	55.0	57.6	58.1	0.0	26.1	0.588249	78148	78221	226-232	1.000000	77.4
rps3-rpl22_p2	rps3-rpl22	2	TGCGAACCAGTATGAACGAT	TCAACCTAGAGCTCAGGGAC	20	20	45.0	55.0	57.3	58.1	0.0	26.1	0.811686	78148	78221	225-231	1.000000	75.6
rps3-rpl22_p3	rps3-rpl22	3	TTGCGAACCAGTATGAACGA	AGGGACGCGGTTATCCTATA	20	20	45.0	50.0	57.6	57.4	0.0	12.5	1.034315	78148	78221	212-218	1.000000	74.0
rps3-rpl22_p4	rps3-rpl22	4	TTGCGAACCAGTATGAACGA	TTCAACCTAGAGCTCAGGGA	20	20	45.0	50.0	57.6	57.4	0.0	26.1	1.065630	78148	78221	227-233	1.000000	73.6
rps3-rpl22_p5	rps3-rpl22	5	TTGCGAACCAGTATGAACGA	GGGACGCGGTTATCCTATAC	20	20	45.0	55.0	57.6	57.4	0.0	12.5	1.086145	78148	78221	211-217	1.000000	73.6
ndhF-rpl21_p1	ndhF-rpl21	1	AAAAAGTGATCCCGATCCCG	CCAATCGATTCGGTGTCAGT	20	20	50.0	50.0	58.0	58.0	2.3	7.9	0.048532	91179	91596	608-608	1.000000	80.8
ndhF-rpl21_p2	ndhF-rpl21	2	GAAACAGTGGTACGAGCCAA	CCAATCGATTCGGTGTCAGT	20	20	50.0	50.0	58.1	58.0	4.5	7.9	0.136447	91179	91596	567-567	1.000000	80.2
ndhF-rpl21_p3	ndhF-rpl21	3	AACGGAAACAGTGGTACGAG	CCAATCGATTCGGTGTCAGT	20	20	50.0	50.0	57.9	58.0	11.6	7.9	0.154627	91179	91596	571-571	1.000000	80.0
ndhF-rpl21_p4	ndhF-rpl21	4	AAAAAGTGATCCCGATCCCG	TCGTGTTTCGTTCCAATCGA	20	20	50.0	45.0	58.0	57.9	2.3	0.0	0.175516	91179	91596	620-620	1.000000	79.8
ndhF-rpl21_p5	ndhF-rpl21	5	GATCCCGGTAGAATGGAAGC	CCAATCGATTCGGTGTCAGT	20	20	55.0	50.0	57.8	58.0	0.0	7.9	0.181486	91179	91596	595-595	1.000000	79.8