primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	atpA	1	ACCTGACCAACTAAGTTGCT	GCTAACCCATTCGCAGGTTA	20	20	45.0	50.0	56.7	58.0	0.0	2.0	1.357846	149	1669	2337-2337	0.500000	49.1
atpA_p2	atpA	2	ACCTGACCAACTAAGTTGCT	AGCCAACAAGTAATTCGCCA	20	20	45.0	45.0	56.7	58.1	0.0	0.0	1.420879	149	1669	1901-1901	0.500000	48.6
atpA_p3	atpA	3	ACCTGACCAACTAAGTTGCT	GTCATGGTCACGGATTTGGT	20	20	45.0	50.0	56.7	58.2	0.0	0.0	1.503949	149	1669	2176-2176	0.500000	48.0
atpA_p4	atpA	4	ACCTGACCAACTAAGTTGCT	CAATTGGTCATGGTCACGGA	20	20	45.0	50.0	56.7	58.2	0.0	0.0	1.505237	149	1669	2182-2182	0.500000	48.0
atpA_p5	atpA	5	ACCTGACCAACTAAGTTGCT	TGGTCATGGTCACGGATTTG	20	20	45.0	50.0	56.7	58.2	0.0	0.0	1.505237	149	1669	2178-2178	0.500000	48.0
rpoA_p1	rpoA	1	CCGATAAAGGTGCTTGAGCT	AAAGTACCCCATTTGCAGCA	20	20	50.0	45.0	58.0	58.0	4.4	0.0	0.038081	20710	21807	1994-1994	0.500000	59.7
rpoA_p2	rpoA	2	CCGATAAAGGTGCTTGAGCT	AAGTACCCCATTTGCAGCAA	20	20	50.0	45.0	58.0	58.0	4.4	2.8	0.038081	20710	21807	1993-1993	0.500000	59.7
rpoA_p3	rpoA	3	CCGATAAAGGTGCTTGAGCT	AGTACCCCATTTGCAGCAAA	20	20	50.0	45.0	58.0	58.0	4.4	22.7	0.038081	20710	21807	1992-1992	0.500000	59.7
rpoA_p4	rpoA	4	TTCTTTTTCACGCGCATCAC	AAGTACCCCATTTGCAGCAA	20	20	45.0	45.0	58.0	58.0	5.7	2.8	0.039279	20710	21807	2169-2169	0.500000	59.7
rpoA_p5	rpoA	5	TTCTTTTTCACGCGCATCAC	AGTACCCCATTTGCAGCAAA	20	20	45.0	45.0	58.0	58.0	5.7	22.7	0.039279	20710	21807	2168-2168	0.500000	59.7
psaJ-trnP(ugg)_p1	psaJ-trnP(ugg)	1	AGCTTCTTTCTAGCCGAACG	TACCAAACTGCGCTACATCC	20	20	50.0	50.0	58.0	58.0	0.0	9.7	0.020873	33783	34253	819-819	0.500000	59.8
psaJ-trnP(ugg)_p2	psaJ-trnP(ugg)	2	AGCTTCTTTCTAGCCGAACG	AACCAAGTGCAAAAACTGGC	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.025095	33783	34253	1280-1280	0.500000	59.8
psaJ-trnP(ugg)_p3	psaJ-trnP(ugg)	3	GCTTCTTTCTAGCCGAACGA	TACCAAACTGCGCTACATCC	20	20	50.0	50.0	58.0	58.0	0.0	9.7	0.026372	33783	34253	818-818	0.500000	59.8
psaJ-trnP(ugg)_p4	psaJ-trnP(ugg)	4	GCTTCTTTCTAGCCGAACGA	AACCAAGTGCAAAAACTGGC	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.030595	33783	34253	1279-1279	0.500000	59.8
psaJ-trnP(ugg)_p5	psaJ-trnP(ugg)	5	AGCTTCTTTCTAGCCGAACG	AATGCAGCAGCTCGTGAATA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.030751	33783	34253	1466-1466	0.500000	59.8
accD_p1	accD	1	CTGGATTCGAACCAGCGTAA	TGCAACTTCCATGGCTTCTT	20	20	50.0	45.0	58.0	58.0	24.2	7.0	0.004934	51037	52182	2119-2119	0.500000	60.0
accD_p2	accD	2	TGGATTCGAACCAGCGTAAG	TGCAACTTCCATGGCTTCTT	20	20	50.0	45.0	58.0	58.0	13.3	7.0	0.004934	51037	52182	2118-2118	0.500000	60.0
accD_p3	accD	3	CATCCCCATTAACCACTCGG	TGCAACTTCCATGGCTTCTT	20	20	55.0	45.0	58.0	58.0	0.0	7.0	0.033033	51037	52182	2076-2076	0.500000	59.7
accD_p4	accD	4	CCATCCCCATTAACCACTCG	TGCAACTTCCATGGCTTCTT	20	20	55.0	45.0	58.0	58.0	0.0	7.0	0.033033	51037	52182	2077-2077	0.500000	59.7
accD_p5	accD	5	TGGATTCGAACCAGCGTAAG	ACGAACCCGAACCAATTTCA	20	20	50.0	45.0	58.0	58.0	13.3	0.0	0.034491	51037	52182	1606-1606	0.500000	59.7
cysA_p1	cysA	1	TGCTGGTCGTCGAGTTATTG	ACTCAGTTGGTAGAGTGGCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.026106	52333	53415	2399-2399	0.500000	59.8
cysA_p2	cysA	2	AAGATGCGCCATTTAGACGT	ACTCAGTTGGTAGAGTGGCT	20	20	45.0	50.0	58.0	58.0	4.2	0.0	0.041080	52333	53415	2247-2247	0.500000	59.7
cysA_p3	cysA	3	AGATGCGCCATTTAGACGTT	ACTCAGTTGGTAGAGTGGCT	20	20	45.0	50.0	58.0	58.0	4.2	0.0	0.041080	52333	53415	2246-2246	0.500000	59.7
cysA_p4	cysA	4	TGCTGGTCGTCGAGTTATTG	GCGGATGTTGCATAGTGGTA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.061097	52333	53415	1738-1738	0.500000	59.5
cysA_p5	cysA	5	AGATGCGCCATTTAGACGTT	GCGGATGTTGCATAGTGGTA	20	20	45.0	50.0	58.0	58.1	4.2	0.0	0.076071	52333	53415	1585-1585	0.500000	59.4
orf205_p1	orf205	1	CAGTGAGCGGATAAGCAGAG	CACAAATCACTTGCGACCAC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.186180	56361	56978	1546-1546	0.500000	58.5
orf205_p2	orf205	2	CAGTGAGCGGATAAGCAGAG	TCACAAATCACTTGCGACCA	20	20	55.0	45.0	58.1	58.0	0.0	0.0	0.189207	56361	56978	1547-1547	0.500000	58.5
orf205_p3	orf205	3	CGGTCAGTAGGTCAGGAGAA	CACAAATCACTTGCGACCAC	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.201062	56361	56978	1631-1631	0.500000	58.4
orf205_p4	orf205	4	TCTTCCGGTCAGTAGGTCAG	CACAAATCACTTGCGACCAC	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.201062	56361	56978	1636-1636	0.500000	58.4
orf205_p5	orf205	5	CAGTGAGCGGATAAGCAGAG	GTAACTGTTCCGCGAAAAGC	20	20	55.0	50.0	58.1	58.1	0.0	8.2	0.203102	56361	56978	1503-1503	0.500000	58.4
orf216_p1	orf216	1	GTGGTCGCAAGTGATTTGTG	TCGGTGGTGGTACTTTAGGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.050566	57960	58610	1553-1553	0.500000	59.6
orf216_p2	orf216	2	GCTTTTCGCGGAACAGTTAC	TCGGTGGTGGTACTTTAGGT	20	20	50.0	50.0	58.1	58.0	6.1	0.0	0.067489	57960	58610	1596-1596	0.500000	59.5
orf216_p3	orf216	3	GTGGTCGCAAGTGATTTGTG	AGCAGCTTGTGAAGTTTGGA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.093694	57960	58610	1390-1390	0.500000	59.3
orf216_p4	orf216	4	CTGAAATCGGAAACTCGGGT	TCGGTGGTGGTACTTTAGGT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.108772	57960	58610	1390-1390	0.500000	59.1
orf216_p5	orf216	5	GCTTTTCGCGGAACAGTTAC	AGCAGCTTGTGAAGTTTGGA	20	20	50.0	45.0	58.1	57.9	6.1	0.0	0.110616	57960	58610	1433-1433	0.500000	59.1
rbcL_p1	rbcL	1	TAGTTTGTGTGGAAAGCCCC	ACTGGTTCGCCTAAAACGTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.052625	58952	60379	2568-2568	0.500000	59.6
rbcL_p2	rbcL	2	TAGTTTGTGTGGAAAGCCCC	TGGTTCGCCTAAAACGTTGA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.059466	58952	60379	2566-2566	0.500000	59.5
rbcL_p3	rbcL	3	ACACACACACAGTCTGTTCC	ACTGGTTCGCCTAAAACGTT	20	20	50.0	45.0	58.0	58.0	1.7	0.0	0.066507	58952	60379	2493-2493	0.500000	59.5
rbcL_p4	rbcL	4	ACACACACACAGTCTGTTCC	TGGTTCGCCTAAAACGTTGA	20	20	50.0	45.0	58.0	58.0	1.7	0.0	0.073347	58952	60379	2491-2491	0.500000	59.4
rbcL_p5	rbcL	5	TGGCTACTTGCTCACGTTTT	ACTGGTTCGCCTAAAACGTT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.080538	58952	60379	2787-2787	0.500000	59.4
atpB_p1	atpB	1	AACTGGTTCGATGTCGTAGC	CCAGACGGCGAATAATACGT	20	20	50.0	50.0	58.0	57.9	2.0	16.6	0.137653	60738	62180	2777-2777	0.500000	58.9
atpB_p2	atpB	2	CTGGTTCGATGTCGTAGCAA	CCAGACGGCGAATAATACGT	20	20	50.0	50.0	58.0	57.9	13.3	16.6	0.139024	60738	62180	2775-2775	0.500000	58.9
atpB_p3	atpB	3	AACTGGTTCGATGTCGTAGC	AGCACGCCATTCTTGTTTTG	20	20	50.0	45.0	58.0	57.9	2.0	0.0	0.146426	60738	62180	2376-2376	0.500000	58.8
atpB_p4	atpB	4	AACTGGTTCGATGTCGTAGC	CAGCACGCCATTCTTGTTTT	20	20	50.0	45.0	58.0	57.9	2.0	0.0	0.146426	60738	62180	2377-2377	0.500000	58.8
atpB_p5	atpB	5	CTGGTTCGATGTCGTAGCAA	AGCACGCCATTCTTGTTTTG	20	20	50.0	45.0	58.0	57.9	13.3	0.0	0.147797	60738	62180	2374-2374	0.500000	58.8
chlI_p1	chlI	1	TTCCAAAGGAAAGGTGTCCG	GATCAGGTGGTGCTAGTTGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.054179	64012	65118	2169-2169	0.500000	59.6
chlI_p2	chlI	2	TAGATGCCATTGCGATTGCT	GATCAGGTGGTGCTAGTTGG	20	20	45.0	55.0	58.0	58.0	11.1	0.0	0.058357	64012	65118	1954-1954	0.500000	59.5
chlI_p3	chlI	3	GAAAGGTGTCCGAGTGGTTT	GATCAGGTGGTGCTAGTTGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.066666	64012	65118	2161-2161	0.500000	59.5
chlI_p4	chlI	4	AAACAGCAAGCCGTACTTCA	GATCAGGTGGTGCTAGTTGG	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.068045	64012	65118	2407-2407	0.500000	59.5
chlI_p5	chlI	5	GTTCGAATCCCTCCCTTTCC	GATCAGGTGGTGCTAGTTGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.069656	64012	65118	2098-2098	0.500000	59.4
psbC-orf105_p1	psbC-orf105	1	CGAGTTCTACGGTCCAACTG	AGGTGCCTAGAGATTCACGA	20	20	55.0	50.0	58.0	57.9	8.8	0.0	0.166855	69357	69983	1608-1608	0.500000	58.7
psbC-orf105_p2	psbC-orf105	2	TGACCAACGTTTAGGTGCAA	AGGTGCCTAGAGATTCACGA	20	20	45.0	50.0	58.0	57.9	14.5	0.0	0.185215	69357	69983	1545-1545	0.500000	58.5
psbC-orf105_p3	psbC-orf105	3	CAGCGGCTGGTTTTGAAAAA	AGGTGCCTAGAGATTCACGA	20	20	45.0	50.0	58.1	57.9	22.1	0.0	0.212150	69357	69983	1153-1153	0.500000	58.3
psbC-orf105_p4	psbC-orf105	4	ATGGCGTTCATTGCTTGTTG	AGGTGCCTAGAGATTCACGA	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.214234	69357	69983	1661-1661	0.500000	58.3
psbC-orf105_p5	psbC-orf105	5	GTTCTACGGTCCAACTGGTC	AGGTGCCTAGAGATTCACGA	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.221376	69357	69983	1605-1605	0.500000	58.2
orf105-psbA_p1	orf105-psbA	1	AAAAAGGCCCTGTACTGTGG	CTTACATGGGTCGTGAGTGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.014769	70302	71124	2203-2203	0.500000	59.9
orf105-psbA_p2	orf105-psbA	2	AAAAAGGCCCTGTACTGTGG	ATGCACCCATTCCACATGTT	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.063247	70302	71124	1997-1997	0.500000	59.5
orf105-psbA_p3	orf105-psbA	3	AAAAAGGCCCTGTACTGTGG	GGTGTAGCAGGTGTATTCGG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.068691	70302	71124	1976-1976	0.500000	59.5
orf105-psbA_p4	orf105-psbA	4	AAAAAGGCCCTGTACTGTGG	GTAGCAGGTGTATTCGGTGG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.068691	70302	71124	1973-1973	0.500000	59.5
orf105-psbA_p5	orf105-psbA	5	AAAAAGGCCCTGTACTGTGG	GGAAGTAATGCACGAGCGTA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.087351	70302	71124	1593-1593	0.500000	59.3
psbA_p1	psbA	1	CTGATAGCTCAGCATAGCCG	AACTGGCTGAGAACCAAGTG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.033586	71125	72183	1855-1855	0.500000	59.7
psbA_p2	psbA	2	CTGATAGCTCAGCATAGCCG	GTGAACTGGCTGAGAACCAA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.039140	71125	72183	1858-1858	0.500000	59.7
psbA_p3	psbA	3	CTGAATAGCAGCTGTCTGGG	AACTGGCTGAGAACCAAGTG	20	20	55.0	50.0	58.0	58.0	16.6	0.0	0.076300	71125	72183	1994-1994	0.500000	59.4
psbA_p4	psbA	4	CTGAATAGCAGCTGTCTGGG	GTGAACTGGCTGAGAACCAA	20	20	55.0	50.0	58.0	58.0	16.6	0.0	0.081853	71125	72183	1997-1997	0.500000	59.3
psbA_p5	psbA	5	CTGATAGCTCAGCATAGCCG	AGTTTCTCGTGAACTGGCTG	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.135378	71125	72183	1866-1866	0.500000	58.9
psbA_p1	psbA	1	AACTGGCTGAGAACCAAGTG	CTGATAGCTCAGCATAGCCG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.033586	96556	97614	1855-1855	0.500000	59.7
psbA_p2	psbA	2	GTGAACTGGCTGAGAACCAA	CTGATAGCTCAGCATAGCCG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.039140	96556	97614	1858-1858	0.500000	59.7
psbA_p3	psbA	3	AACTGGCTGAGAACCAAGTG	CTGAATAGCAGCTGTCTGGG	20	20	50.0	55.0	58.0	58.0	0.0	16.6	0.076300	96556	97614	1994-1994	0.500000	59.4
psbA_p4	psbA	4	GTGAACTGGCTGAGAACCAA	CTGAATAGCAGCTGTCTGGG	20	20	50.0	55.0	58.0	58.0	0.0	16.6	0.081853	96556	97614	1997-1997	0.500000	59.3
psbA_p5	psbA	5	AGTTTCTCGTGAACTGGCTG	CTGATAGCTCAGCATAGCCG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.135378	96556	97614	1866-1866	0.500000	58.9