primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA-trnH-GUG_p1	psbA-trnH-GUG	1	CCTCTAGACCTAGCTGCTGT	CAATCCACCGCCTTTATCCA	20	20	55.0	50.0	57.9	57.9	13.4	0.0	0.117233	1290	1553	296-354	1.000000	91.1
psbA-trnH-GUG_p2	psbA-trnH-GUG	2	CCTCTAGACCTAGCTGCTGT	AATCCACCGCCTTTATCCAC	20	20	55.0	50.0	57.9	57.9	13.4	0.0	0.118681	1290	1553	295-353	1.000000	91.1
psbA-trnH-GUG_p3	psbA-trnH-GUG	3	CCTCTAGACCTAGCTGCTGT	ACAATCCACCGCCTTTATCC	20	20	55.0	50.0	57.9	57.9	13.4	0.0	0.118681	1290	1553	297-355	1.000000	91.1
psbA-trnH-GUG_p4	psbA-trnH-GUG	4	TTCCCTCTAGACCTAGCTGC	CAATCCACCGCCTTTATCCA	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.126063	1290	1553	299-357	1.000000	91.0
psbA-trnH-GUG_p5	psbA-trnH-GUG	5	TTCCCTCTAGACCTAGCTGC	AATCCACCGCCTTTATCCAC	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.127511	1290	1553	298-356	1.000000	91.0
rpoB-clpP_p1	rpoB-clpP	1	CACGAGCCCATATTCTTGCT	GGCCAGGATAAAAGATGCCA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.112736	25925	26942	1817-1892	0.809524	82.3
rpoB-clpP_p2	rpoB-clpP	2	CACGAGCCCATATTCTTGCT	TGTCATCGTTATCGTCGTCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.122294	25925	26942	1959-2034	0.761905	80.7
rpoB-clpP_p3	rpoB-clpP	3	AGAGGTTCCCAGGGAATTCA	TGGCGATGTCTTCCTGAATG	20	20	50.0	50.0	57.9	58.0	24.7	0.0	0.088176	25925	26942	1850-1925	0.714286	79.4
rpoB-clpP_p4	rpoB-clpP	4	CACGAGCCCATATTCTTGCT	TGGCGATGTCTTCCTGAATG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.069378	25925	26942	2025-2100	0.714286	78.7
rpoB-clpP_p5	rpoB-clpP	5	CCGGGGCTTTGCAATATTTG	TGGCGATGTCTTCCTGAATG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.089795	25925	26942	1903-1978	0.666667	76.4
clpP_p1	clpP	1	GCGGAAGAGGGCTTTATGAA	TGACCCTGTTAGTCCGTTCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.043757	26943	27641	1646-1790	1.000000	91.6
clpP_p2	clpP	2	TGCAGTTCGATTCTGGACAG	TGACCCTGTTAGTCCGTTCT	20	20	50.0	50.0	57.9	58.0	16.2	0.0	0.089980	26943	27641	1589-1709	1.000000	91.3
clpP_p3	clpP	3	GCGGAAGAGGGCTTTATGAA	CGAGTACATATTCCTCGGCG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.073303	26943	27641	1357-1444	0.952381	89.5
clpP_p4	clpP	4	ATTTGGGTTCTGGGGTTGAC	TGACCCTGTTAGTCCGTTCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.015052	26943	27641	1518-1605	0.809524	84.3
clpP_p5	clpP	5	ATTTGGGTTCTGGGGTTGAC	CGAGTACATATTCCTCGGCG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.044598	26943	27641	1172-1259	0.809524	84.0
accD_p1	accD	1	AAGTTAGGACTCCGCTGAAT	ACTATCCATCCGCTTGTTACT	20	21	45.0	42.9	56.3	56.5	0.0	0.0	4.221633	39282	41738	2650-2892	0.619048	43.0
accD_p2	accD	2	AGTTAGGACTCCGCTGAATT	ACTATCCATCCGCTTGTTACT	20	21	45.0	42.9	56.3	56.5	0.0	0.0	4.221633	39282	41738	2649-2891	0.619048	43.0
accD_p3	accD	3	AGTTAGGACTCCGCTGAATT	TCGAATAACTATCCATCCGCT	20	21	45.0	42.9	56.3	56.4	0.0	0.0	4.332933	39282	41738	2656-2898	0.619048	42.1
accD_p4	accD	4	TTTCTTCTTATGCCACCCCT	ACTATCCATCCGCTTGTTACT	20	21	45.0	42.9	56.4	56.5	0.0	0.0	4.070272	39282	41738	2928-2948	0.095238	17.5
accD_p5	accD	5	TTTCTTCTTATGCCACCCCT	TCGAATAACTATCCATCCGCT	20	21	45.0	42.9	56.4	56.4	0.0	0.0	4.181572	39282	41738	2935-2955	0.095238	16.6
ycf3-psaA_p1	ycf3-psaA	1	AGCGCAGAATTGGTTGAAGA	GCCCATTCCTCGAAAGAAGT	20	20	45.0	50.0	58.1	57.9	11.5	4.1	0.219123	56472	57448	1055-1264	1.000000	90.2
ycf3-psaA_p2	ycf3-psaA	2	TGAAGCGCAGAATTGGTTGA	GCCCATTCCTCGAAAGAAGT	20	20	45.0	50.0	58.4	57.9	11.5	4.1	0.511886	56472	57448	1058-1267	1.000000	87.9
ycf3-psaA_p3	ycf3-psaA	3	TTGAAGCGCAGAATTGGTTG	GCCCATTCCTCGAAAGAAGT	20	20	45.0	50.0	57.6	57.9	11.5	4.1	0.535971	56472	57448	1059-1268	1.000000	87.7
ycf3-psaA_p4	ycf3-psaA	4	AGCGCAGAATTGGTTGAAGA	GAAGTTTTGACGGGATCCCT	20	20	45.0	50.0	58.1	57.5	11.5	28.4	0.595455	56472	57448	1040-1249	1.000000	87.2
ycf3-psaA_p5	ycf3-psaA	5	AGCGCAGAATTGGTTGAAGA	TGTTCTTGAGAAATGGCCGG	20	20	45.0	50.0	58.1	58.5	11.5	0.0	0.570155	56472	57448	1122-1289	0.952381	82.9
trnT-GGU-trnE-UUC_p1	trnT-GGU-trnE-UUC	1	GAACCGATGACTTACGCCTT	CGTTGCCTCCTTGAAAGAGA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.175803	68204	69028	587-971	1.000000	90.6
trnT-GGU-trnE-UUC_p2	trnT-GGU-trnE-UUC	2	TACCGCTGAGTTAAAAGGGC	CGTTGCCTCCTTGAAAGAGA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.273369	68204	69028	552-936	1.000000	89.8
trnT-GGU-trnE-UUC_p3	trnT-GGU-trnE-UUC	3	CCGATGACTTACGCCTTACC	CGTTGCCTCCTTGAAAGAGA	20	20	55.0	50.0	58.2	57.8	0.0	0.0	0.320736	68204	69028	584-968	1.000000	89.4
trnT-GGU-trnE-UUC_p4	trnT-GGU-trnE-UUC	4	GAACCGATGACTTACGCCTT	TCGTAATACCCTACCCCCAG	20	20	50.0	55.0	58.0	57.6	0.0	0.0	0.392800	68204	69028	622-1006	1.000000	88.9
trnT-GGU-trnE-UUC_p5	trnT-GGU-trnE-UUC	5	GAACCGATGACTTACGCCTT	ATCCCCGATCCACTTCCTTT	20	20	50.0	50.0	58.0	58.4	0.0	0.0	0.409475	68204	69028	641-1025	0.904762	84.9
trnY-GUA-trnD-GUC_p1	trnY-GUA-trnD-GUC	1	GGGGACGGACTGTAAATTCG	CGGTGCTCTGACCAATTGAA	20	20	55.0	50.0	58.4	58.5	0.0	7.1	0.828828	69245	69755	562-607	1.000000	85.4
trnY-GUA-trnD-GUC_p2	trnY-GUA-trnD-GUC	2	GGGACGGACTGTAAATTCGT	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	57.6	58.5	0.0	7.1	0.852505	69245	69755	561-606	1.000000	85.2
trnY-GUA-trnD-GUC_p3	trnY-GUA-trnD-GUC	3	GGGGACGGACTGTAAATTCG	TTGAATACGTCGGGACTGAC	20	20	55.0	50.0	58.4	57.4	0.0	0.0	0.993634	69245	69755	640-661	0.952381	82.1
trnY-GUA-trnD-GUC_p4	trnY-GUA-trnD-GUC	4	TGGGGACGGACTGTAAATTC	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	57.2	58.5	0.0	7.1	1.251324	69245	69755	563-608	1.000000	82.0
trnY-GUA-trnD-GUC_p5	trnY-GUA-trnD-GUC	5	GGGACGGACTGTAAATTCGT	TTGAATACGTCGGGACTGAC	20	20	50.0	50.0	57.6	57.4	0.0	0.0	1.017311	69245	69755	639-660	0.952381	82.0
psbM-petN_p1	psbM-petN	1	TGCTACTGCACTGTTCATTCT	ATGGGGAAGAAGTGGACTCT	21	20	42.9	50.0	57.6	57.6	0.0	0.0	1.758286	70961	72346	1118-1522	1.000000	77.9
psbM-petN_p2	psbM-petN	2	TGCTACTGCACTGTTCATTC	ATGGGGAAGAAGTGGACTCT	20	20	45.0	50.0	56.1	57.6	0.0	0.0	2.280835	70961	72346	1118-1522	1.000000	73.8
psbM-petN_p3	psbM-petN	3	TGCTACTGCACTGTTCATTCT	TGGGCTGCTTTAATGGTAGTC	21	21	42.9	47.6	57.6	58.0	0.0	0.0	2.408092	70961	72346	1165-1569	1.000000	72.7
psbM-petN_p4	psbM-petN	4	TGCTACTGCACTGTTCATTCT	TGGGGAAGAAGTGGACTCTA	21	20	42.9	50.0	57.6	57.0	0.0	0.0	2.421497	70961	72346	1117-1521	1.000000	72.6
psbM-petN_p5	psbM-petN	5	GCTACTGCACTGTTCATTCT	ATGGGGAAGAAGTGGACTCT	20	20	45.0	50.0	55.8	57.6	0.0	0.0	2.580161	70961	72346	1117-1521	1.000000	71.4
trnC-GCA-psbB_p1	trnC-GCA-psbB	1	CGACACCCAAGATTCGAACT	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.317578	72886	73757	746-1965	1.000000	89.5
trnC-GCA-psbB_p2	trnC-GCA-psbB	2	AAGATTCGAACTGGGGAACG	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.332055	72886	73757	738-1957	1.000000	89.3
trnC-GCA-psbB_p3	trnC-GCA-psbB	3	ACGAGGATTTGCAGTCCTTC	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	57.9	58.2	20.0	0.0	0.337897	72886	73757	721-1940	1.000000	89.3
trnC-GCA-psbB_p4	trnC-GCA-psbB	4	ACACCCAAGATTCGAACTGG	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	57.8	58.2	13.4	0.0	0.422297	72886	73757	744-1963	1.000000	88.6
trnC-GCA-psbB_p5	trnC-GCA-psbB	5	AACTGGGGAACGAGGATTTG	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.437665	72886	73757	855-1949	0.952381	86.6
rps8-rpl14_p1	rps8-rpl14	1	ATTCCTTTTCCGCCCATGTC	GAGGAATTGAGGCAGGTCAA	20	20	50.0	50.0	58.5	57.5	0.0	0.0	1.022569	82119	82335	341-363	0.952381	78.4
rps8-rpl14_p2	rps8-rpl14	2	GATTCCTTTTCCGCCCATGT	GAGGAATTGAGGCAGGTCAA	20	20	50.0	50.0	58.5	57.5	0.0	0.0	1.022569	82119	82335	342-364	0.952381	78.4
rps8-rpl14_p3	rps8-rpl14	3	ATTCCTTTTCCGCCCATGTC	TCCAAAGGGAAGTCGCATTT	20	20	50.0	45.0	58.5	57.7	0.0	0.0	0.812035	82119	82335	378-379	0.095238	37.7
rps8-rpl14_p4	rps8-rpl14	4	GATTCCTTTTCCGCCCATGT	TCCAAAGGGAAGTCGCATTT	20	20	50.0	45.0	58.5	57.7	0.0	0.0	0.812035	82119	82335	379-380	0.095238	37.7
rps8-rpl14_p5	rps8-rpl14	5	GCCCATGTCAGCATTTCGTA	TCCAAAGGGAAGTCGCATTT	20	20	50.0	45.0	58.6	57.7	0.0	0.0	0.907834	82119	82335	367-368	0.095238	37.0
ycf1_p1	ycf1	1	GGCAGAAAACCCTTACCCAA	TGCCATGCCTTCTCTGTTTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.004289	105229	105348	1301-1301	0.095238	44.0
ycf1_p2	ycf1	2	GGCAGAAAACCCTTACCCAA	CGATAACGGTGCGCTGTATA	20	20	50.0	50.0	58.0	58.0	0.0	13.1	0.044651	105229	105348	907-907	0.095238	43.7
ycf1_p3	ycf1	3	GGCAGAAAACCCTTACCCAA	CAAGCTTACTTGTCTTGCGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048175	105229	105348	1271-1271	0.095238	43.7
ycf1_p4	ycf1	4	ACGAACGTCTTGCAACTCTT	TGCCATGCCTTCTCTGTTTT	20	20	45.0	45.0	58.1	58.0	0.0	0.0	0.064419	105229	105348	1027-1027	0.095238	43.5
ycf1_p5	ycf1	5	AACACTGGAATCGCCCATTT	TGCCATGCCTTCTCTGTTTT	20	20	45.0	45.0	58.1	58.0	0.0	0.0	0.076996	105229	105348	994-994	0.095238	43.4
ndhF-rpl32_p1	ndhF-rpl32	1	CTGGAAGTGTAACGAAAGGT	GCTGCCCAAGATCCTTTTCT	20	20	45.0	50.0	55.3	58.2	0.0	0.0	2.829880	111564	112092	580-689	0.904762	65.6
ndhF-rpl32_p2	ndhF-rpl32	2	AAAGTAGGAGTCCCATTCCT	GCTGCCCAAGATCCTTTTCT	20	20	45.0	50.0	55.1	58.2	5.2	0.0	3.051778	111564	112092	613-722	0.904762	63.7
ndhF-rpl32_p3	ndhF-rpl32	3	AAAAGTAGGAGTCCCATTCCT	GCTGCCCAAGATCCTTTTCT	21	20	42.9	50.0	55.9	58.2	5.2	0.0	3.211968	111564	112092	614-723	0.904762	62.5
ndhF-rpl32_p4	ndhF-rpl32	4	AAAAGTAGGAGTCCCATTCC	GCTGCCCAAGATCCTTTTCT	20	20	45.0	50.0	54.3	58.2	5.2	0.0	3.840977	111564	112092	614-723	0.904762	57.4
ndhF-rpl32_p5	ndhF-rpl32	5	CTGGAAGTGTAACGAAAGGTA	GCTGCCCAAGATCCTTTTCT	21	20	42.9	50.0	55.3	58.2	0.0	0.0	3.860922	111564	112092	580-689	0.904762	57.3
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AAAAAGAAAAACCTGGGCTA	AGCGTGTCTACCGATTTCAC	20	20	35.0	50.0	53.1	58.0	0.0	0.0	4.878824	112267	113018	772-831	0.952381	51.1
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AAAAGAAAAACCTGGGCTAA	AGCGTGTCTACCGATTTCAC	20	20	35.0	50.0	53.1	58.0	0.0	0.0	4.878824	112267	113018	771-830	0.952381	51.1
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AAAAAGAAAAACCTGGGCTA	CAGCGTGTCTACCGATTTCA	20	20	35.0	50.0	53.1	58.0	0.0	0.0	4.880195	112267	113018	773-832	0.952381	51.1
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AAAAGAAAAACCTGGGCTAA	CAGCGTGTCTACCGATTTCA	20	20	35.0	50.0	53.1	58.0	0.0	0.0	4.880195	112267	113018	772-831	0.952381	51.1
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AAAAAGAAAAACCTGGGCTAA	AGCGTGTCTACCGATTTCAC	21	20	33.3	50.0	54.1	58.0	0.0	0.0	4.957176	112267	113018	772-831	0.952381	50.4