primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CATTGAGTTAGCAACCCGGA	TCTTGAAAAGGGGGCTCAAC	20	20	50.0	50.0	57.9	58.0	0.7	0.0	0.112574	3916	4465	663-670	1.000000	84.7
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	ACCATTGAGTTAGCAACCCG	TCTTGAAAAGGGGGCTCAAC	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.183407	3916	4465	665-672	1.000000	84.2
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CATTGAGTTAGCAACCCGGA	GAAAAGGGGGCTCAACCTAC	20	20	50.0	55.0	57.9	58.2	0.7	0.0	0.272456	3916	4465	659-666	1.000000	83.5
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CATTGAGTTAGCAACCCGGA	GGGGCTCAACCTACAAGAAC	20	20	50.0	55.0	57.9	58.2	0.7	0.0	0.285444	3916	4465	653-660	1.000000	83.5
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	ACCATTGAGTTAGCAACCCG	GAAAAGGGGGCTCAACCTAC	20	20	50.0	55.0	58.2	58.2	0.0	0.0	0.343289	3916	4465	661-668	1.000000	82.9
trnY-GUA-trnD-GUC_p1	trnY-GUA-trnD-GUC	1	AATTCTTCCTGGGTCGATGC	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	58.0	58.5	0.0	7.1	0.521454	16587	16955	482-493	1.000000	84.5
trnY-GUA-trnD-GUC_p2	trnY-GUA-trnD-GUC	2	GGGGACGGACTGTAAATTCG	CGGTGCTCTGACCAATTGAA	20	20	55.0	50.0	58.4	58.5	0.0	7.1	0.828828	16587	16955	449-460	1.000000	82.6
trnY-GUA-trnD-GUC_p3	trnY-GUA-trnD-GUC	3	GGGACGGACTGTAAATTCGT	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	57.6	58.5	0.0	7.1	0.852505	16587	16955	448-459	1.000000	82.5
trnY-GUA-trnD-GUC_p4	trnY-GUA-trnD-GUC	4	AATTCTTCCTGGGTCGATGC	GGTGCTCTGACCAATTGAAC	20	20	50.0	50.0	58.0	57.0	0.0	7.1	1.041608	16587	16955	481-492	1.000000	80.3
trnY-GUA-trnD-GUC_p5	trnY-GUA-trnD-GUC	5	TGGGGACGGACTGTAAATTC	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	57.2	58.5	0.0	7.1	1.251324	16587	16955	450-461	1.000000	79.2
trnD-GUC-psbM_p1	trnD-GUC-psbM	1	TTCAATTGGTCAGAGCACCG	TCATTTTGGCTGGCTGTTTT	20	20	50.0	40.0	58.5	56.7	5.4	2.0	1.775052	17030	18107	773-1249	1.000000	75.3
trnD-GUC-psbM_p2	trnD-GUC-psbM	2	TTCAATTGGTCAGAGCACCG	AATCATTTTGGCTGGCTGTT	20	20	50.0	40.0	58.5	56.5	5.4	2.0	2.006868	17030	18107	775-1251	1.000000	73.5
trnD-GUC-psbM_p3	trnD-GUC-psbM	3	TTCAATTGGTCAGAGCACCG	ATCATTTTGGCTGGCTGTTT	20	20	50.0	40.0	58.5	56.5	5.4	2.0	2.006868	17030	18107	774-1250	1.000000	73.5
trnD-GUC-psbM_p4	trnD-GUC-psbM	4	TTCAATTGGTCAGAGCACCG	TCATTTTGGCTGGCTGTTTTT	20	21	50.0	38.1	58.5	57.4	5.4	2.0	2.034560	17030	18107	773-1249	1.000000	73.3
trnD-GUC-psbM_p5	trnD-GUC-psbM	5	TTCAATTGGTCAGAGCACCG	TTTGGCTGGCTGTTTTTACA	20	20	50.0	40.0	58.5	56.3	5.4	2.0	2.128044	17030	18107	769-1245	1.000000	72.5
psbM-petN_p1	psbM-petN	1	AAAACAGCCAGCCAAAATGA	TAGTAAGTCTCGCTTGGGCT	20	20	40.0	50.0	56.7	58.2	0.0	0.0	1.449068	18213	19031	881-929	1.000000	78.2
psbM-petN_p2	psbM-petN	2	AAAACAGCCAGCCAAAATGA	GGCTGCTTTAATGGTGGTCT	20	20	40.0	50.0	56.7	58.2	0.0	0.0	1.458296	18213	19031	865-913	1.000000	78.2
psbM-petN_p3	psbM-petN	3	AAAACAGCCAGCCAAAATGA	GTAGTATGGGGGAGGAGTGG	20	20	40.0	60.0	56.7	58.3	0.0	0.0	1.579440	18213	19031	825-873	1.000000	77.5
psbM-petN_p4	psbM-petN	4	AACAGCCAGCCAAAATGATT	TAGTAAGTCTCGCTTGGGCT	20	20	40.0	50.0	56.5	58.2	0.0	0.0	1.680884	18213	19031	879-927	1.000000	76.3
psbM-petN_p5	psbM-petN	5	AAAACAGCCAGCCAAAATGA	TAGTATGGGGGAGGAGTGGA	20	20	40.0	55.0	56.7	58.4	0.0	0.0	1.669873	18213	19031	824-872	0.956522	74.7
trnC-GCA-rpoB_p1	trnC-GCA-rpoB	1	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	20	20	50.0	55.0	58.1	58.3	14.6	0.0	0.395580	20123	21346	1264-1325	1.000000	87.4
trnC-GCA-rpoB_p2	trnC-GCA-rpoB	2	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	20	20	50.0	55.0	58.1	58.3	14.6	0.0	0.395580	20123	21346	1263-1324	1.000000	87.4
trnC-GCA-rpoB_p3	trnC-GCA-rpoB	3	AAAGGATTTGCAGTCCCCTG	AGTTCTTCTGCCAAGCCTTG	20	20	50.0	50.0	58.1	58.4	14.6	0.0	0.457296	20123	21346	1327-1388	1.000000	86.9
trnC-GCA-rpoB_p4	trnC-GCA-rpoB	4	AAAGGATTTGCAGTCCCCTG	AAGTTCTTCTGCCAAGCCTT	20	20	50.0	45.0	58.1	57.6	14.6	0.0	0.442790	20123	21346	1328-1387	0.956522	84.9
trnC-GCA-rpoB_p5	trnC-GCA-rpoB	5	AAAGGATTTGCAGTCCCCTG	CAAGTTCTTCTGCCAAGCCT	20	20	50.0	50.0	58.1	58.4	14.6	0.0	0.457296	20123	21346	1329-1388	0.956522	84.7
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TTGGCTACAATCGAGGAGGT	TTCCAATGCTACGCCTTGAA	20	20	50.0	45.0	58.4	57.8	0.0	5.6	0.624160	45729	46332	681-728	1.000000	82.1
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	TTGGCTACAATCGAGGAGGT	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	58.4	58.2	0.0	0.0	0.582844	45729	46332	731-778	1.000000	81.9
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	GGCTACAATCGAGGAGGTCT	TTCCAATGCTACGCCTTGAA	20	20	55.0	45.0	58.6	57.8	0.0	5.6	0.787821	45729	46332	679-726	1.000000	80.8
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	GGCTACAATCGAGGAGGTCT	ACGGAAAGAGAGGGATTCGA	20	20	55.0	50.0	58.6	58.2	0.0	0.0	0.746506	45729	46332	729-776	1.000000	80.6
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TTGGCTACAATCGAGGAGGT	AGTTCCAATGCTACGCCTTG	20	20	50.0	50.0	58.4	58.5	0.0	0.0	0.978341	45729	46332	683-730	1.000000	79.2
rbcL-psaI_p1	rbcL-psaI	1	GGCTTCAGGGGGTATTCATG	CCATTGCAATTGCCGGAAAT	20	20	55.0	45.0	58.0	58.0	0.0	26.9	0.040121	57935	58876	1273-1353	1.000000	89.7
rbcL-psaI_p2	rbcL-psaI	2	TCTCATTATTGCCGCGACAA	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	58.0	58.0	11.0	26.9	0.044141	57935	58876	1563-1643	1.000000	89.0
rbcL-psaI_p3	rbcL-psaI	3	TTGTCTCATTATTGCCGCGA	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	58.0	58.0	8.2	26.9	0.044141	57935	58876	1566-1646	1.000000	88.9
rbcL-psaI_p4	rbcL-psaI	4	GGCTTCAGGGGGTATTCATG	AATCGGTCATAACCACTGCC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.051504	57935	58876	1941-2027	0.956522	87.0
rbcL-psaI_p5	rbcL-psaI	5	TCTCATTATTGCCGCGACAA	AATCGGTCATAACCACTGCC	20	20	45.0	50.0	58.0	58.0	11.0	0.0	0.055525	57935	58876	2231-2317	0.956522	86.5
trnI-CAU-trnL-CAA_p1	trnI-CAU-trnL-CAA	1	TCTCAGACAGTAGTGCCACA	TGAAACGTGGCGATGATGAT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.027388	87091	87210	1129-1129	0.173913	57.9
trnI-CAU-trnL-CAA_p2	trnI-CAU-trnL-CAA	2	AGTCTCCCCTCTCTCCCTTA	TGAAACGTGGCGATGATGAT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.026012	87091	87210	428-428	0.173913	57.4
trnI-CAU-trnL-CAA_p3	trnI-CAU-trnL-CAA	3	AGTCTCCCCTCTCTCCCTTA	CTGAGACGAAGAGAAAGGGC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.006444	87091	87210	763-763	0.130435	45.2
trnI-CAU-trnL-CAA_p4	trnI-CAU-trnL-CAA	4	TCTCAGACAGTAGTGCCACA	CTGAGACGAAGAGAAAGGGC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.007820	87091	87210	1464-1464	0.130435	45.2
trnI-CAU-trnL-CAA_p5	trnI-CAU-trnL-CAA	5	CGCTACGGATATCCTTCAGC	CTGAGACGAAGAGAAAGGGC	20	20	55.0	55.0	58.0	58.0	19.6	0.0	0.019224	87091	87210	918-918	0.130435	45.1
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	ATGGGGGCTTATCCTATCCC	TCCTAAGAGCAGCGTGTCTA	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.137198	108472	109007	972-1002	0.826087	81.9
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	ATGGGGGCTTATCCTATCCC	AGCAGCGTGTCTACCAATTT	20	20	55.0	45.0	58.0	57.8	0.0	0.0	0.197925	108472	109007	965-995	0.826087	81.5
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	ATGGGGGCTTATCCTATCCC	ATGTCCCGAAGAAACCCAAC	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.107283	108472	109007	1285-1315	0.826087	80.4
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	ATGGGGGCTTATCCTATCCC	CCGAAGAAACCCAACGAGAA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.139223	108472	109007	1280-1310	0.826087	80.2
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	ATGGGGGCTTATCCTATCCC	GCAGATAATAAGGAGCCGCA	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.182753	108472	109007	1563-1593	0.826087	78.7
ccsA-ndhD_p1	ccsA-ndhD	1	AGGTTTACATAGTTATGGTTCA	CTCTCGCTATCCGTTGACAG	22	20	31.8	55.0	52.5	57.9	4.3	0.0	7.597928	110134	110324	286-295	1.000000	51.0
ccsA-ndhD_p2	ccsA-ndhD	2	AGGTTTACATAGTTATGGTTCA	TCTCTCGCTATCCGTTGACA	22	20	31.8	50.0	52.5	58.3	4.3	0.0	7.738984	110134	110324	287-296	1.000000	51.0
ccsA-ndhD_p3	ccsA-ndhD	3	AGGAATAGGTTTACATAGTTATGGT	CTCTCGCTATCCGTTGACAG	25	20	32.0	55.0	55.3	57.9	4.3	0.0	7.838974	110134	110324	292-301	1.000000	51.0
ccsA-ndhD_p4	ccsA-ndhD	4	AGGTTTACATAGTTATGGTTCAT	CTCTCGCTATCCGTTGACAG	23	20	30.4	55.0	53.2	57.9	4.3	0.0	7.942467	110134	110324	286-295	1.000000	51.0
ccsA-ndhD_p5	ccsA-ndhD	5	AGGTTTACATAGTTATGGTTCA	CCGTTGACAGGGTAGAGACT	22	20	31.8	55.0	52.5	58.5	4.3	0.0	7.938493	110134	110324	276-281	0.956522	48.9
ndhG-ndhI_p1	ndhG-ndhI	1	CCCAGCGAAAAGGCAGAATA	TTGAGCCGGTTACCAATCTC	20	20	50.0	50.0	58.2	57.6	0.0	1.9	0.646397	113734	113931	375-437	1.000000	83.1
ndhG-ndhI_p2	ndhG-ndhI	2	CCCAGCGAAAAGGCAGAATA	AGCCGGTTACCAATCTCCAT	20	20	50.0	50.0	58.2	58.8	0.0	1.9	1.036511	113734	113931	372-434	1.000000	80.0
ndhG-ndhI_p3	ndhG-ndhI	3	CCCAGCGAAAAGGCAGAATA	GCCGGTTACCAATCTCCATA	20	20	50.0	50.0	58.2	57.1	0.0	1.9	1.172547	113734	113931	371-433	1.000000	78.9
ndhG-ndhI_p4	ndhG-ndhI	4	CCCAGCGAAAAGGCAGAATA	GCCGGTTACCAATCTCCATAA	20	21	50.0	47.6	58.2	57.8	0.0	1.9	1.452636	113734	113931	371-433	1.000000	76.7
ndhG-ndhI_p5	ndhG-ndhI	5	CCCAGCGAAAAGGCAGAATA	AGCCGGTTACCAATCTCCA	20	19	50.0	52.6	58.2	58.3	0.0	1.9	1.558144	113734	113931	372-434	1.000000	75.8
trnL-CAA-trnI-CAU_p1	trnL-CAA-trnI-CAU	1	TGAAACGTGGCGATGATGAT	AGTCTCCCCTCTCTCCCTTA	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.026012	135862	135981	428-428	0.173913	56.6
trnL-CAA-trnI-CAU_p2	trnL-CAA-trnI-CAU	2	CTGAGACGAAGAGAAAGGGC	AGTCTCCCCTCTCTCCCTTA	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.006444	135862	135981	763-763	0.130435	45.2
trnL-CAA-trnI-CAU_p3	trnL-CAA-trnI-CAU	3	CTGAGACGAAGAGAAAGGGC	CGCTACGGATATCCTTCAGC	20	20	55.0	55.0	58.0	58.0	0.0	19.6	0.019224	135862	135981	918-918	0.130435	45.1
trnL-CAA-trnI-CAU_p4	trnL-CAA-trnI-CAU	4	CTGAGACGAAGAGAAAGGGC	TAGAGATGGACTACGGGCAG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.020536	135862	135981	661-661	0.130435	45.1
trnL-CAA-trnI-CAU_p5	trnL-CAA-trnI-CAU	5	CTGAGACGAAGAGAAAGGGC	TTTCGCTAGCTCACATGCAT	20	20	55.0	45.0	58.0	58.0	0.0	13.9	0.031616	135862	135981	953-953	0.130435	45.0