primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-psbA_p1	trnH-psbA	1	GATCCACTTGGCTACATCCG	TCCCTCTAGACTTAGCTGCT	20	20	55.0	50.0	58.1	56.9	0.0	7.6	1.263417	75	405	400-508	1.000000	73.4
trnH-psbA_p2	trnH-psbA	2	CAATCCACTGCCTTGATCCA	TCCCTCTAGACTTAGCTGCT	20	20	50.0	50.0	57.9	56.9	0.0	7.6	1.267778	75	405	414-522	1.000000	73.3
trnH-psbA_p3	trnH-psbA	3	AATCCACTGCCTTGATCCAC	TCCCTCTAGACTTAGCTGCT	20	20	50.0	50.0	57.9	56.9	0.0	7.6	1.269286	75	405	413-521	1.000000	73.3
trnH-psbA_p4	trnH-psbA	4	ACAATCCACTGCCTTGATCC	TCCCTCTAGACTTAGCTGCT	20	20	50.0	50.0	57.9	56.9	0.0	7.6	1.269286	75	405	415-523	1.000000	73.3
trnH-psbA_p5	trnH-psbA	5	GATCCACTTGGCTACATCCG	TTCCCTCTAGACTTAGCTGCT	20	21	55.0	47.6	58.1	57.6	0.0	7.6	1.519095	75	405	401-509	1.000000	71.4
psbA-matK_p1	psbA-matK	1	CAGACGGAAACTAAGCTCCC	GAGCGCTTTGGCCGTATATA	20	20	55.0	50.0	58.0	57.9	0.0	13.3	0.086087	1468	2027	1283-1298	1.000000	81.8
psbA-matK_p2	psbA-matK	2	CCAGACGGAAACTAAGCTCC	GAGCGCTTTGGCCGTATATA	20	20	55.0	50.0	58.0	57.9	0.0	13.3	0.086087	1468	2027	1284-1299	1.000000	81.8
psbA-matK_p3	psbA-matK	3	CCCAGACGGAAACTAAGCTC	GAGCGCTTTGGCCGTATATA	20	20	55.0	50.0	58.0	57.9	0.0	13.3	0.086087	1468	2027	1285-1300	1.000000	81.8
psbA-matK_p4	psbA-matK	4	CGGAAACTAAGCTCCCACTC	GAGCGCTTTGGCCGTATATA	20	20	55.0	50.0	58.0	57.9	0.0	13.3	0.086087	1468	2027	1279-1294	1.000000	81.8
psbA-matK_p5	psbA-matK	5	CGGAAACTAAGCTCCCACTC	GTACGCGGTCAAATGCTAGA	20	20	55.0	50.0	58.0	58.1	0.0	12.5	0.094267	1468	2027	1456-1471	1.000000	81.8
matK-rps16_p1	matK-rps16	1	GTTGTGCCCCAAAAATGGAG	CCCAGAACAAGGAAACACCA	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.234224	3543	4961	2264-2288	1.000000	81.0
matK-rps16_p2	matK-rps16	2	TTGTGCCCCAAAAATGGAGT	CCCAGAACAAGGAAACACCA	20	20	45.0	50.0	58.2	57.9	0.0	0.0	0.265965	3543	4961	2263-2287	1.000000	80.7
matK-rps16_p3	matK-rps16	3	GTTGTGCCCCAAAAATGGAG	GCCGTACGAGGAGAAAACTT	20	20	50.0	50.0	57.8	57.9	0.0	18.8	0.244305	3543	4961	1965-1994	1.000000	80.6
matK-rps16_p4	matK-rps16	4	TTGTGCCCCAAAAATGGAGT	GCCGTACGAGGAGAAAACTT	20	20	45.0	50.0	58.2	57.9	0.0	18.8	0.276046	3543	4961	1964-1993	1.000000	80.4
matK-rps16_p5	matK-rps16	5	GTTGTGCCCCAAAAATGGAG	AGCCGTCTATCGAATCGTTG	20	20	50.0	50.0	57.8	57.9	0.0	1.5	0.289442	3543	4961	1897-1925	1.000000	80.3
trnS-GCU-psbM_p1	trnS-GCU-psbM	1	CTTTCGTCCACTCAGCCATC	ACGTAAAAACAGCCAGTCAA	20	20	55.0	40.0	58.6	55.8	0.0	0.0	2.817728	8395	10778	2287-2438	1.000000	69.5
trnS-GCU-psbM_p2	trnS-GCU-psbM	2	CTTTCGTCCACTCAGCCATC	ACGTAAAAACAGCCAGTCAAA	20	21	55.0	38.1	58.6	56.6	0.0	0.0	3.045161	8395	10778	2287-2438	1.000000	67.6
trnS-GCU-psbM_p3	trnS-GCU-psbM	3	CTTTCGTCCACTCAGCCATC	TGCTACTGCACTGTTCATTT	20	20	55.0	40.0	58.6	55.6	0.0	0.0	3.058849	8395	10778	2337-2488	1.000000	67.5
trnS-GCU-psbM_p4	trnS-GCU-psbM	4	TTTCGTCCACTCAGCCATCT	ACGTAAAAACAGCCAGTCAA	20	20	50.0	40.0	59.0	55.8	0.0	0.0	3.204831	8395	10778	2286-2437	1.000000	66.4
trnS-GCU-psbM_p5	trnS-GCU-psbM	5	CTTTCGTCCACTCAGCCATC	TGCTACTGCACTGTTCATTTT	20	21	55.0	38.1	58.6	56.4	0.0	0.0	3.270660	8395	10778	2337-2488	1.000000	65.8
psbM-rpoB_p1	psbM-rpoB	1	TTGACTGGCTGTTTTTACGT	CTTCTGCCAAACCCTGATCA	20	20	40.0	50.0	55.8	57.8	0.0	0.0	2.386494	10884	12900	2189-2251	1.000000	72.9
psbM-rpoB_p2	psbM-rpoB	2	TTTGACTGGCTGTTTTTACGT	CTTCTGCCAAACCCTGATCA	21	20	38.1	50.0	56.6	57.8	0.0	0.0	2.613927	10884	12900	2190-2252	1.000000	71.1
psbM-rpoB_p3	psbM-rpoB	3	TTGACTGGCTGTTTTTACGT	TCTTCTGCCAAACCCTGATC	20	20	40.0	50.0	55.8	57.5	0.0	0.0	2.678371	10884	12900	2190-2252	1.000000	70.6
psbM-rpoB_p4	psbM-rpoB	4	TTGACTGGCTGTTTTTACGT	CTCTTCTGCCAAACCCTGAT	20	20	40.0	50.0	55.8	57.5	0.0	0.0	2.685367	10884	12900	2191-2253	1.000000	70.5
psbM-rpoB_p5	psbM-rpoB	5	TTGACTGGCTGTTTTTACGT	TGCCAAACCCTGATCAATGA	20	20	40.0	45.0	55.8	57.4	0.0	0.0	2.782712	10884	12900	2185-2247	1.000000	69.7
atpl-atpH_p1	atpl-atpH	1	CCCGTCATGTTCCTTGGATT	ATAACGGAAGCGGCAGAAAT	20	20	50.0	45.0	57.9	58.0	0.0	1.4	0.167157	25121	26276	1290-1338	1.000000	81.0
atpl-atpH_p2	atpl-atpH	2	CCCGTCATGTTCCTTGGATT	TACCTTGACCAACTCCAGGT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.241050	25121	26276	1346-1394	1.000000	80.3
atpl-atpH_p3	atpl-atpH	3	CCCGTCATGTTCCTTGGATT	AGCCAATCCAGCAGCAATAA	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.277216	25121	26276	1306-1354	1.000000	80.1
atpl-atpH_p4	atpl-atpH	4	CCCGTCATGTTCCTTGGATT	AATAACGGAAGCGGCAGAAA	20	20	50.0	45.0	57.9	58.2	0.0	1.4	0.312696	25121	26276	1291-1339	1.000000	79.8
atpl-atpH_p5	atpl-atpH	5	CCCGTCATGTTCCTTGGATT	GCAGTACCTTGACCAACTCC	20	20	50.0	55.0	57.9	58.2	0.0	0.0	0.319087	25121	26276	1350-1398	1.000000	79.7
psbC-trnS-UGA_p1	psbC-trnS-UGA	1	CCATGACCCCGCTTAATTGA	GAGAGATGGCTGAGTGGTTG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.072690	35379	35642	353-366	1.000000	86.5
psbC-trnS-UGA_p2	psbC-trnS-UGA	2	TCCATGACCCCGCTTAATTG	GAGAGATGGCTGAGTGGTTG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.072690	35379	35642	354-367	1.000000	86.5
psbC-trnS-UGA_p3	psbC-trnS-UGA	3	GGTGGAGTAGCTACCGAGAT	GAGAGATGGCTGAGTGGTTG	20	20	55.0	55.0	58.0	58.0	20.3	0.0	0.037289	35379	35642	534-547	1.000000	84.4
psbC-trnS-UGA_p4	psbC-trnS-UGA	4	GGTGGAGTAGCTACCGAGAT	GTCTTTCGGAGAGATGGCTG	20	20	55.0	55.0	58.0	58.1	20.3	1.2	0.082688	35379	35642	542-555	1.000000	84.0
psbC-trnS-UGA_p5	psbC-trnS-UGA	5	TCTAAGGGGTCCAAATGGGT	GAGAGATGGCTGAGTGGTTG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.090187	35379	35642	652-665	1.000000	83.3
ycf3_p1	ycf3	1	TATTCCAAATCACGCGAGCA	TTCTGAAATTGCGGAGGCTT	20	20	45.0	45.0	58.0	58.1	15.9	0.0	0.107438	42708	42741	503-503	1.000000	84.4
ycf3_p2	ycf3	2	CGCGAGCAGACATTAGCTAT	TTCTGAAATTGCGGAGGCTT	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.147803	42708	42741	491-491	1.000000	84.2
ycf3_p3	ycf3	3	TATTCCAAATCACGCGAGCA	ATTCTGAAATTGCGGAGGCT	20	20	45.0	45.0	58.0	57.9	15.9	0.0	0.151927	42708	42741	504-504	1.000000	84.0
ycf3_p4	ycf3	4	TATTCCAAATCACGCGAGCA	GCCAGGGGGATTCTGAAATT	20	20	45.0	50.0	58.0	57.8	15.9	0.0	0.182495	42708	42741	513-513	1.000000	83.7
ycf3_p5	ycf3	5	ACACATACAAAGAGACCCGC	TTCTGAAATTGCGGAGGCTT	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.185062	42708	42741	853-853	1.000000	82.3
ndhC-atpE_p1	ndhC-atpE	1	TCATATTCGTAAAGCAGAAACA	CAAATCTAGCTCTCCGACGG	22	20	31.8	55.0	53.9	57.9	0.0	7.8	6.249440	50544	52634	2173-2219	1.000000	43.0
ndhC-atpE_p2	ndhC-atpE	2	TCATATTCGTAAAGCAGAAACA	GGCAAATCTAGCTCTCCGAC	22	20	31.8	55.0	53.9	58.1	0.0	0.0	6.263143	50544	52634	2175-2221	1.000000	43.0
ndhC-atpE_p3	ndhC-atpE	3	TCATATTCGTAAAGCAGAAACA	CGAGGCAAATCTAGCTCTCC	22	20	31.8	55.0	53.9	57.9	0.0	0.0	6.269962	50544	52634	2178-2224	1.000000	43.0
ndhC-atpE_p4	ndhC-atpE	4	TCATATTCGTAAAGCAGAAACA	GAGGCAAATCTAGCTCTCCG	22	20	31.8	55.0	53.9	57.9	0.0	0.0	6.269962	50544	52634	2177-2223	1.000000	43.0
ndhC-atpE_p5	ndhC-atpE	5	TCATATTCGTAAAGCAGAAACA	AGGCAAATCTAGCTCTCCGA	22	20	31.8	50.0	53.9	58.2	0.0	0.0	6.340740	50544	52634	2176-2222	1.000000	43.0
petA-psbJ_p1	petA-psbJ	1	ACGTGTACAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	58.0	57.6	17.6	4.1	0.411974	63334	64199	919-1004	1.000000	82.6
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTCGC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.9	57.6	0.0	4.1	0.416851	63334	64199	909-994	1.000000	82.6
petA-psbJ_p3	petA-psbJ	3	ACGTGTACAAGGCCTTTTGT	TGTGATCGGTTTAGTAGGCG	20	20	45.0	50.0	58.0	57.4	17.6	0.0	0.619192	63334	64199	957-1042	1.000000	80.9
petA-psbJ_p4	petA-psbJ	4	ACGTGTACAAGGCCTTTTGT	GTGATCGGTTTAGTAGGCGT	20	20	45.0	50.0	58.0	57.4	17.6	0.0	0.620943	63334	64199	956-1041	1.000000	80.9
petA-psbJ_p5	petA-psbJ	5	GGCCTTTTGTTCTTCTTCGC	TGTGATCGGTTTAGTAGGCG	20	20	50.0	50.0	57.9	57.4	0.0	0.0	0.624069	63334	64199	947-1032	1.000000	80.9
psbL-psbF_p1	psbL-psbF	1	ATAACCCCCAGTAGAGGCTG	GCCTAGCTGTACCTACCGTT	20	20	55.0	55.0	58.2	58.9	0.0	0.0	1.082577	64588	64609	154-154	1.000000	71.9
psbL-psbF_p2	psbL-psbF	2	ATAACCCCCAGTAGAGGCTG	GGGGTCAATATCAGCAATGC	20	20	55.0	50.0	58.2	56.9	0.0	0.0	1.286639	64588	64609	126-126	1.000000	70.3
psbL-psbF_p3	psbL-psbF	3	ATAACCCCCAGTAGAGGCTG	TTTACAGTGCGATGGTTGGC	20	20	55.0	50.0	58.2	59.1	0.0	0.0	1.313770	64588	64609	182-182	1.000000	69.9
psbL-psbF_p4	psbL-psbF	4	ATAACCCCCAGTAGAGGCTG	GCCTAGCTGTACCTACCGT	20	19	55.0	57.9	58.2	58.2	0.0	0.0	1.387637	64588	64609	154-154	1.000000	69.4
psbL-psbF_p5	psbL-psbF	5	AATAACCCCCAGTAGAGGCT	GCCTAGCTGTACCTACCGTT	20	20	50.0	55.0	57.4	58.9	0.0	0.0	1.479316	64588	64609	155-155	1.000000	68.7
psaJ-rpl33_p1	psaJ-rpl33	1	ATGCATTGACGTTCCCCTTT	AGAACCGGTATTCGCACATC	20	20	45.0	50.0	58.1	58.1	0.0	8.0	0.140712	67505	67954	511-514	1.000000	83.7
psaJ-rpl33_p2	psaJ-rpl33	2	ATGCATTGACGTTCCCCTTT	GAACCGGTATTCGCACATCT	20	20	45.0	50.0	58.1	58.1	0.0	8.0	0.140712	67505	67954	510-513	1.000000	83.7
psaJ-rpl33_p3	psaJ-rpl33	3	ACATATCTTTCCGTGGCACC	AGAACCGGTATTCGCACATC	20	20	50.0	50.0	58.0	58.1	12.9	8.0	0.102454	67505	67954	599-602	1.000000	83.3
psaJ-rpl33_p4	psaJ-rpl33	4	ACATATCTTTCCGTGGCACC	GAACCGGTATTCGCACATCT	20	20	50.0	50.0	58.0	58.1	12.9	8.0	0.102454	67505	67954	598-601	1.000000	83.3
psaJ-rpl33_p5	psaJ-rpl33	5	ACATATCTTTCCGTGGCACC	TCGCACATCTTTACCCTTGG	20	20	50.0	50.0	58.0	57.9	12.9	0.0	0.146337	67505	67954	588-591	1.000000	83.0
clpP-psbB_p1	clpP-psbB	1	TCCTCTCCAGGACTTCGAAA	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	57.7	58.2	0.0	0.0	0.541514	72351	72849	535-566	1.000000	82.0
clpP-psbB_p2	clpP-psbB	2	TCCTCTCCAGGACTTCGAAA	AGCAACCAAAGCTGTATGCA	20	20	50.0	45.0	57.7	58.4	0.0	10.2	0.684567	72351	72849	608-639	1.000000	80.6
clpP-psbB_p3	clpP-psbB	3	TCCTCTCCAGGACTTCGAAA	ACACGATACCAAGGCAAACC	20	20	50.0	50.0	57.7	58.5	0.0	0.0	0.773898	72351	72849	538-569	1.000000	80.1
clpP-psbB_p4	clpP-psbB	4	GATGCATCTTCCTCTCCAGG	CGATACCAAGGCAAACCCAT	20	20	55.0	50.0	57.5	58.2	19.7	0.0	0.784592	72351	72849	544-575	1.000000	80.0
clpP-psbB_p5	clpP-psbB	5	TCCTCTCCAGGACTTCGAAA	TGAACACGATACCAAGGCAA	20	20	50.0	45.0	57.7	57.4	0.0	0.0	0.856821	72351	72849	541-572	1.000000	79.4
ndhI-ndhG_p1	ndhI-ndhG	1	TTGATCACATCCGAGCGTTT	AGACCACGGAAGTTACTCCA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.098676	118145	118514	1037-1069	1.000000	81.5
ndhI-ndhG_p2	ndhI-ndhG	2	TTGATCACATCCGAGCGTTT	AGACTTGTACAGACCACGGA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.102185	118145	118514	1047-1079	1.000000	81.4
ndhI-ndhG_p3	ndhI-ndhG	3	TGTGAAGTATGCGTTCGTGT	AGACCACGGAAGTTACTCCA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.148378	118145	118514	977-1009	1.000000	81.2
ndhI-ndhG_p4	ndhI-ndhG	4	GCTTGTGAAGTATGCGTTCG	AGACCACGGAAGTTACTCCA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.150423	118145	118514	980-1012	1.000000	81.2
ndhI-ndhG_p5	ndhI-ndhG	5	TGTGAAGTATGCGTTCGTGT	AGACTTGTACAGACCACGGA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.151886	118145	118514	987-1019	1.000000	81.2
ndhD-ccsA_p1	ndhD-ccsA	1	AGGTATTGGTATGTACCCCGA	TGGCGACTCTAGGCTTTCTT	21	20	47.6	50.0	57.7	58.7	6.7	0.0	2.066192	121670	121910	421-430	1.000000	69.2
ndhD-ccsA_p2	ndhD-ccsA	2	AGGTATTGGTATGTACCCCGA	CAATTGTGGCGACTCTAGGC	21	20	47.6	55.0	57.7	59.0	6.7	0.0	2.323484	121670	121910	427-436	1.000000	67.1
ndhD-ccsA_p3	ndhD-ccsA	3	AGGTATTGGTATGTACCCCGA	GCAATTGTGGCGACTCTAGG	21	20	47.6	55.0	57.7	59.0	6.7	7.4	2.323484	121670	121910	428-437	1.000000	67.1
ndhD-ccsA_p4	ndhD-ccsA	4	AGGTATTGGTATGTACCCCGA	TGGCGACTCTAGGCTTTCT	21	19	47.6	52.6	57.7	58.0	6.7	0.0	2.355736	121670	121910	421-430	1.000000	66.8
ndhD-ccsA_p5	ndhD-ccsA	5	TCAGTTGACAAGGTTGAAGGT	TGGCGACTCTAGGCTTTCTT	21	20	42.9	50.0	57.4	58.7	0.0	0.0	2.377845	121670	121910	384-393	1.000000	66.8
rpl32-ndhF_p1	rpl32-ndhF	1	TCCAAATATTTTTACGAATACGCT	GTTGCCGTCGGAAAAAGAAG	24	20	29.2	50.0	55.3	58.0	0.0	17.5	6.751912	123774	124866	1217-1252	1.000000	47.1
rpl32-ndhF_p2	rpl32-ndhF	2	TCCAAATATTTTTACGAATACGCT	TGCCGTCGGAAAAAGAAGAA	24	20	29.2	45.0	55.3	58.0	0.0	17.5	6.763201	123774	124866	1215-1250	1.000000	47.1
rpl32-ndhF_p3	rpl32-ndhF	3	TCCAAATATTTTTACGAATACGCT	TTGCCGTCGGAAAAAGAAGA	24	20	29.2	45.0	55.3	58.0	0.0	17.5	6.763201	123774	124866	1216-1251	1.000000	47.1
rpl32-ndhF_p4	rpl32-ndhF	4	TCCAAATATTTTTACGAATACGCT	CTTTTTGTTGCCGTCGGAAA	24	20	29.2	45.0	55.3	57.8	0.0	17.5	6.915793	123774	124866	1223-1258	1.000000	47.1
rpl32-ndhF_p5	rpl32-ndhF	5	TCCAAATATTTTTACGAATACGCT	TTTGTTGCCGTCGGAAAAAG	24	20	29.2	45.0	55.3	57.8	0.0	17.5	6.915793	123774	124866	1220-1255	1.000000	47.1
ndhF-trnN_p1	ndhF-trnN	1	TGTGGGGGTGAGAAAATTGG	GGCAGAATAGCAGAGCAAGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.043772	127098	128655	1425-2074	1.000000	84.3
ndhF-trnN_p2	ndhF-trnN	2	TGTGGGGGTGAGAAAATTGG	GTCCCCCATCAGCGTATTTT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.061025	127098	128655	1485-2134	1.000000	84.2
ndhF-trnN_p3	ndhF-trnN	3	TGTGGGGGTGAGAAAATTGG	GAACTTGGGTGTGGGTCTTT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.075608	127098	128655	1575-2224	1.000000	84.2
ndhF-trnN_p4	ndhF-trnN	4	TGTGGGGGTGAGAAAATTGG	AACGCGTTCCTCGTCATTAA	20	20	50.0	45.0	58.0	57.9	0.0	20.8	0.077079	127098	128655	1382-2031	1.000000	84.1
ndhF-trnN_p5	ndhF-trnN	5	GGGGGTGGACGTATTTCTTC	GGCAGAATAGCAGAGCAAGT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.069634	127098	128655	1307-1956	1.000000	83.9