primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	rbcL-accD	1	TTGAATGCTACTGCGGGTAC	GTTTCCTCCAGCTTCTCGAG	20	20	50.0	55.0	58.0	58.0	0.0	5.9	0.021738	55098	56048	2252-2252	0.500000	59.8
rbcL-accD_p2	rbcL-accD	2	TGCAATGCACGCTGTTATTG	GTTTCCTCCAGCTTCTCGAG	20	20	45.0	55.0	58.0	58.0	2.7	5.9	0.023300	55098	56048	2085-2085	0.500000	59.8
rbcL-accD_p3	rbcL-accD	3	TCATGGTATGCACTTCCGTG	GTTTCCTCCAGCTTCTCGAG	20	20	50.0	55.0	58.0	58.0	15.5	5.9	0.025961	55098	56048	2052-2052	0.500000	59.8
rbcL-accD_p4	rbcL-accD	4	AATGAGGGACGTGATCTTGC	GTTTCCTCCAGCTTCTCGAG	20	20	50.0	55.0	58.0	58.0	0.0	5.9	0.033035	55098	56048	1676-1676	0.500000	59.7
rbcL-accD_p5	rbcL-accD	5	CTTAACAGGGGGATTCACCG	GTTTCCTCCAGCTTCTCGAG	20	20	55.0	55.0	58.0	58.0	0.0	5.9	0.041583	55098	56048	2163-2163	0.500000	59.7
accD_p1	accD	1	CCGCCAGTAATAATCCCAGG	GGAGGTACAAGCAAAGACCA	20	20	55.0	50.0	57.8	57.4	0.0	0.0	0.746482	56049	57752	2341-2341	0.500000	54.0
accD_p2	accD	2	ATGCCGCCAGTAATAATCCC	GGAGGTACAAGCAAAGACCA	20	20	50.0	50.0	57.8	57.4	0.0	0.0	0.761851	56049	57752	2344-2344	0.500000	53.9
accD_p3	accD	3	GGGATGCCGCCAGTAATAAT	GGAGGTACAAGCAAAGACCA	20	20	50.0	50.0	57.8	57.4	0.0	0.0	0.761851	56049	57752	2347-2347	0.500000	53.9
accD_p4	accD	4	AATCTTAGCCGTTGCATCGA	GGAGGTACAAGCAAAGACCA	20	20	45.0	50.0	57.7	57.4	18.4	0.0	0.871006	56049	57752	2817-2817	0.500000	53.0
accD_p5	accD	5	CCGCCAGTAATAATCCCAGG	CCTTTGAACTTCCCGAAAGC	20	20	55.0	50.0	57.8	57.3	0.0	0.0	0.891559	56049	57752	1957-1957	0.500000	52.9
psaJ-rpl33_p1	psaJ-rpl33	1	TCCAGATGCATTAACATTTCCCT	CGAACATCTTTACCCTTTGCC	23	21	39.1	47.6	58.1	57.5	0.0	0.0	4.616927	66222	66653	477-486	1.000000	50.4
psaJ-rpl33_p2	psaJ-rpl33	2	TCCAGATGCATTAACATTTCCC	CGAACATCTTTACCCTTTGCC	22	21	40.9	47.6	56.7	57.5	0.0	0.0	4.760951	66222	66653	477-486	1.000000	49.2
psaJ-rpl33_p3	psaJ-rpl33	3	CCAGATGCATTAACATTTCCCT	CGAACATCTTTACCCTTTGCC	22	21	40.9	47.6	56.7	57.5	0.0	0.0	4.769184	66222	66653	476-485	1.000000	49.2
psaJ-rpl33_p4	psaJ-rpl33	4	TTCCAGATGCATTAACATTTCCC	CGAACATCTTTACCCTTTGCC	23	21	39.1	47.6	57.4	57.5	0.0	0.0	5.084398	66222	66653	478-487	1.000000	47.3
psaJ-rpl33_p5	psaJ-rpl33	5	CCAGATGCATTAACATTTCCCTT	CGAACATCTTTACCCTTTGCC	23	21	39.1	47.6	57.4	57.5	0.0	0.0	5.090733	66222	66653	476-485	1.000000	47.3
trnN-GUU-rps15_p1	trnN-GUU-rps15	1	CCCGGAACTTCTTCGTAGTG	ACGAACATCATGAGCTGTCC	20	20	55.0	50.0	58.0	58.0	0.7	0.1	0.023566	109712	109831	587-702	1.000000	84.5
trnN-GUU-rps15_p2	trnN-GUU-rps15	2	CCCGGAACTTCTTCGTAGTG	GGAAGAGATAAGAGGGCCCT	20	20	55.0	55.0	58.0	57.9	0.7	29.2	0.118583	109712	109831	484-599	1.000000	84.2
trnN-GUU-rps15_p3	trnN-GUU-rps15	3	CGTAGTGTCTCCGTTCCATG	ACGAACATCATGAGCTGTCC	20	20	55.0	50.0	58.1	58.0	0.0	0.1	0.106360	109712	109831	574-689	1.000000	84.0
trnN-GUU-rps15_p4	trnN-GUU-rps15	4	CCCGGAACTTCTTCGTAGTG	CGTGTTGTTGCATCGAAACA	20	20	55.0	45.0	58.0	57.9	0.7	6.2	0.106045	109712	109831	861-976	1.000000	83.9
trnN-GUU-rps15_p5	trnN-GUU-rps15	5	CAATTCCCGTTCTCAACCCA	ACGAACATCATGAGCTGTCC	20	20	50.0	50.0	58.1	58.0	0.0	0.1	0.111449	109712	109831	641-756	1.000000	83.6
ndhA_p1	ndhA	1	AGTTGAAGCTCCAAAAGGGG	CAGCTCGTAGGGTTTGTTGA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.164894	117309	119480	2508-2508	0.500000	58.7
ndhA_p2	ndhA	2	AGTTGAAGCTCCAAAAGGGG	AGCTCGTAGGGTTTGTTGAC	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.166395	117309	119480	2507-2507	0.500000	58.7
ndhA_p3	ndhA	3	GTTGAAGCTCCAAAAGGGGA	CAGCTCGTAGGGTTTGTTGA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.168442	117309	119480	2507-2507	0.500000	58.7
ndhA_p4	ndhA	4	GTTGAAGCTCCAAAAGGGGA	AGCTCGTAGGGTTTGTTGAC	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.169942	117309	119480	2506-2506	0.500000	58.6
ndhA_p5	ndhA	5	AGTTGAAGCTCCAAAAGGGG	CCAATATACCTGGCAGCTCG	20	20	50.0	55.0	58.0	58.2	0.0	9.6	0.200270	117309	119480	2521-2521	0.500000	58.4
ndhI_p1	ndhI	1	ATTTGTGGCGTCAACCGATA	GTGCAGCAGCTACAAAATGG	20	20	45.0	50.0	57.9	58.0	0.0	1.8	0.115372	119565	120068	1554-1554	0.500000	59.1
ndhI_p2	ndhI	2	ATTTGTGGCGTCAACCGATA	CCATTCCCAACGGTCCATAG	20	20	45.0	55.0	57.9	58.0	0.0	0.0	0.134751	119565	120068	1663-1663	0.500000	58.9
ndhI_p3	ndhI	3	ATTTGTGGCGTCAACCGATA	GCCCCCACGTAAATAAGGAG	20	20	45.0	55.0	57.9	58.0	0.0	0.0	0.138898	119565	120068	1576-1576	0.500000	58.9
ndhI_p4	ndhI	4	ATTTGTGGCGTCAACCGATA	TAACCCCGTACCATGACGTA	20	20	45.0	50.0	57.9	57.9	0.0	6.2	0.238368	119565	120068	1740-1740	0.500000	58.1
ndhI_p5	ndhI	5	ATTTGTGGCGTCAACCGATA	GTTTATGGCCCCCACGTAAA	20	20	45.0	50.0	57.9	58.2	0.0	0.0	0.265268	119565	120068	1583-1583	0.500000	57.9
ndhG_p1	ndhG	1	TAACCTCTGAACGTTTCCGC	TCGATTGGTTGATGCGAGTT	20	20	50.0	45.0	58.2	57.9	0.5	0.0	0.306294	120387	120929	1749-1749	0.500000	57.5
ndhG_p2	ndhG	2	TAACCTCTGAACGTTTCCGC	CGATTGGTTGATGCGAGTTG	20	20	50.0	50.0	58.2	57.8	0.5	0.0	0.376813	120387	120929	1748-1748	0.500000	57.0
ndhG_p3	ndhG	3	TAACCTCTGAACGTTTCCGC	GCGGCTGCAATTGCTATAAC	20	20	50.0	50.0	58.2	58.2	0.5	29.4	0.454204	120387	120929	1675-1675	0.500000	56.4
ndhG_p4	ndhG	4	TAACCTCTGAACGTTTCCGC	AGCGGCTGCAATTGCTATAA	20	20	50.0	45.0	58.2	58.3	0.5	29.4	0.533849	120387	120929	1676-1676	0.500000	55.7
ndhG_p5	ndhG	5	TCTGCCTGTTGTTGATTGGA	TCGATTGGTTGATGCGAGTT	20	20	45.0	45.0	57.3	57.9	0.0	0.0	0.748396	120387	120929	1661-1661	0.500000	54.0
ndhG-ndhE_p1	ndhG-ndhE	1	CCATTTTGTAGCTGCTGCAC	ACATCTCTTACAACCGACGC	20	20	50.0	50.0	58.0	58.0	20.1	0.0	0.021132	120930	121166	1325-1325	0.500000	59.8
ndhG-ndhE_p2	ndhG-ndhE	2	CCATTTTGTAGCTGCTGCAC	CATCTCTTACAACCGACGCA	20	20	50.0	50.0	58.0	58.0	20.1	0.0	0.022503	120930	121166	1324-1324	0.500000	59.8
ndhG-ndhE_p3	ndhG-ndhE	3	TCTGTGGCTCGTCAACAAAA	ACATCTCTTACAACCGACGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.039089	120930	121166	976-976	0.500000	59.7
ndhG-ndhE_p4	ndhG-ndhE	4	TTCTGTGGCTCGTCAACAAA	ACATCTCTTACAACCGACGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.039089	120930	121166	977-977	0.500000	59.7
ndhG-ndhE_p5	ndhG-ndhE	5	TTTCTGTGGCTCGTCAACAA	ACATCTCTTACAACCGACGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.039089	120930	121166	978-978	0.500000	59.7
ndhE-psaC_p1	ndhE-psaC	1	TCTGTGGCTCGTCAACAAAA	ACATCTCTTACAACCGACGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.039089	121473	121713	976-976	0.500000	59.7
ndhE-psaC_p2	ndhE-psaC	2	TTCTGTGGCTCGTCAACAAA	ACATCTCTTACAACCGACGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.039089	121473	121713	977-977	0.500000	59.7
ndhE-psaC_p3	ndhE-psaC	3	TTTCTGTGGCTCGTCAACAA	ACATCTCTTACAACCGACGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.039089	121473	121713	978-978	0.500000	59.7
ndhE-psaC_p4	ndhE-psaC	4	TCTGTGGCTCGTCAACAAAA	CATCTCTTACAACCGACGCA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.040459	121473	121713	975-975	0.500000	59.7
ndhE-psaC_p5	ndhE-psaC	5	TTCTGTGGCTCGTCAACAAA	CATCTCTTACAACCGACGCA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.040459	121473	121713	976-976	0.500000	59.7
ndhD_p1	ndhD	1	GCGTCGGTTGTAAGAGATGT	GGCATTCTTTCAGGAGCTGT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.176250	122172	123614	2237-2237	0.500000	58.6
ndhD_p2	ndhD	2	TGCGTCGGTTGTAAGAGATG	GGCATTCTTTCAGGAGCTGT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.177621	122172	123614	2238-2238	0.500000	58.6
ndhD_p3	ndhD	3	GCGTCGGTTGTAAGAGATGT	TCAGGAGCTGTATGGGCTAA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.182516	122172	123614	2228-2228	0.500000	58.5
ndhD_p4	ndhD	4	GCGTCGGTTGTAAGAGATGT	TTCAGGAGCTGTATGGGCTA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.182516	122172	123614	2229-2229	0.500000	58.5
ndhD_p5	ndhD	5	TGCGTCGGTTGTAAGAGATG	TCAGGAGCTGTATGGGCTAA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.183886	122172	123614	2229-2229	0.500000	58.5
ndhD-ccsA_p1	ndhD-ccsA	1	TTATCGATGGCTTCCCTTGC	CTCTCCAGTCCCAGTGGTTA	20	20	50.0	55.0	58.0	58.1	8.5	8.7	0.094097	123615	123837	1170-1170	0.500000	59.2
ndhD-ccsA_p2	ndhD-ccsA	2	CCCTGGTTGATGGTAGTTGG	CTCTCCAGTCCCAGTGGTTA	20	20	55.0	55.0	57.9	58.1	0.0	8.7	0.190186	123615	123837	1479-1479	0.500000	58.5
ndhD-ccsA_p3	ndhD-ccsA	3	TTATCGATGGCTTCCCTTGC	GGCATTCTTTCAGGAGCTGT	20	20	50.0	50.0	58.0	58.2	8.5	0.0	0.201101	123615	123837	841-841	0.500000	58.4
ndhD-ccsA_p4	ndhD-ccsA	4	TTATCGATGGCTTCCCTTGC	TCAGGAGCTGTATGGGCTAA	20	20	50.0	50.0	58.0	57.8	8.5	0.0	0.207367	123615	123837	832-832	0.500000	58.3
ndhD-ccsA_p5	ndhD-ccsA	5	TTATCGATGGCTTCCCTTGC	TTCAGGAGCTGTATGGGCTA	20	20	50.0	50.0	58.0	57.8	8.5	0.0	0.207367	123615	123837	833-833	0.500000	58.3
ccsA_p1	ccsA	1	TTATCGATGGCTTCCCTTGC	GTTTTGAACAAGTTGCCGCT	20	20	50.0	45.0	58.0	58.1	8.5	1.8	0.111438	123838	124818	1769-1769	0.500000	59.1
ccsA_p2	ccsA	2	TTATCGATGGCTTCCCTTGC	AAGTTGCCGCTATGGTGAAA	20	20	50.0	45.0	58.0	58.1	8.5	0.0	0.128395	123838	124818	1760-1760	0.500000	59.0
ccsA_p3	ccsA	3	TTATCGATGGCTTCCCTTGC	TAGACACGCTGCTCTTAGGA	20	20	50.0	50.0	58.0	57.9	8.5	0.0	0.156959	123838	124818	1736-1736	0.500000	58.7
ccsA_p4	ccsA	4	TTATCGATGGCTTCCCTTGC	AGTTGCCGCTATGGTGAAAT	20	20	50.0	45.0	58.0	57.9	8.5	0.0	0.161452	123838	124818	1759-1759	0.500000	58.7
ccsA_p5	ccsA	5	TTATCGATGGCTTCCCTTGC	AAATTGGTAGACACGCTGCT	20	20	50.0	45.0	58.0	57.8	8.5	0.0	0.217686	123838	124818	1743-1743	0.500000	58.3
trnL-UAG-ndhF_p1	trnL-UAG-ndhF	1	TAACCACTGGGACTGGAGAG	AACGGGTCGTGTAAACCAAA	20	20	55.0	45.0	58.1	58.0	0.0	0.0	0.096945	125007	126600	2715-2715	0.500000	59.2
trnL-UAG-ndhF_p2	trnL-UAG-ndhF	2	TAACCACTGGGACTGGAGAG	ACGGGTCGTGTAAACCAAAA	20	20	55.0	45.0	58.1	58.0	0.0	0.0	0.096945	125007	126600	2714-2714	0.500000	59.2
trnL-UAG-ndhF_p3	trnL-UAG-ndhF	3	TAACCACTGGGACTGGAGAG	CCACATACGTCGGAGGTTTT	20	20	55.0	50.0	58.1	57.9	0.0	8.8	0.150365	125007	126600	2325-2325	0.500000	58.8
trnL-UAG-ndhF_p4	trnL-UAG-ndhF	4	TCCTAAGAGCAGCGTGTCTA	AACGGGTCGTGTAAACCAAA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.159807	125007	126600	2149-2149	0.500000	58.7
trnL-UAG-ndhF_p5	trnL-UAG-ndhF	5	TCCTAAGAGCAGCGTGTCTA	ACGGGTCGTGTAAACCAAAA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.159807	125007	126600	2148-2148	0.500000	58.7
ndhF_p1	ndhF	1	ACGTTTGAAATCCGTACGAGA	ATTCAACAGGCTCTGGAAGG	21	20	42.9	50.0	57.7	57.5	18.8	7.1	1.791207	126601	128841	2947-2947	0.500000	45.7
ndhF_p2	ndhF	2	ACGTTTGAAATCCGTACGAGA	AACAGGCTCTGGAAGGACTT	21	20	42.9	50.0	57.7	58.6	18.8	7.1	1.847769	126601	128841	2943-2943	0.500000	45.2
ndhF_p3	ndhF	3	ACGTTTGAAATCCGTACGAGA	TTCAACAGGCTCTGGAAGGA	21	20	42.9	50.0	57.7	58.6	18.8	7.1	1.853124	126601	128841	2946-2946	0.500000	45.2
ndhF_p4	ndhF	4	ACGTTTGAAATCCGTACGAG	ATTCAACAGGCTCTGGAAGG	20	20	45.0	50.0	56.2	57.5	18.8	7.1	2.287975	126601	128841	2947-2947	0.500000	41.7
ndhF_p5	ndhF	5	ACGTTTGAAATCCGTACGAGA	ACAGGCTCTGGAAGGACTTC	21	20	42.9	55.0	57.7	59.0	18.8	3.5	2.306889	126601	128841	2942-2942	0.500000	41.5
rps15-trnN-GUU_p1	rps15-trnN-GUU	1	ACGAACATCATGAGCTGTCC	CCCGGAACTTCTTCGTAGTG	20	20	50.0	55.0	58.0	58.0	0.1	0.7	0.023566	136068	136187	587-702	1.000000	84.5
rps15-trnN-GUU_p2	rps15-trnN-GUU	2	GGAAGAGATAAGAGGGCCCT	CCCGGAACTTCTTCGTAGTG	20	20	55.0	55.0	57.9	58.0	29.2	0.7	0.118583	136068	136187	484-599	1.000000	84.2
rps15-trnN-GUU_p3	rps15-trnN-GUU	3	ACGAACATCATGAGCTGTCC	CGTAGTGTCTCCGTTCCATG	20	20	50.0	55.0	58.0	58.1	0.1	0.0	0.106360	136068	136187	574-689	1.000000	84.0
rps15-trnN-GUU_p4	rps15-trnN-GUU	4	CGTGTTGTTGCATCGAAACA	CCCGGAACTTCTTCGTAGTG	20	20	45.0	55.0	57.9	58.0	6.2	0.7	0.106045	136068	136187	861-976	1.000000	83.9
rps15-trnN-GUU_p5	rps15-trnN-GUU	5	ACGAACATCATGAGCTGTCC	CAATTCCCGTTCTCAACCCA	20	20	50.0	50.0	58.0	58.1	0.1	0.0	0.111449	136068	136187	641-756	1.000000	83.6