primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	GGGCCCGATCCAATAACAAT	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.0	58.0	5.7	7.6	0.028964	4448	5929	1667-1796	1.000000	85.8
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	GGGCCCGATCCAATAACAAT	TGAGGTTGCGAATACGAGTG	20	20	50.0	50.0	58.0	58.0	5.7	0.0	0.013825	4448	5929	2019-2148	1.000000	85.7
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CCTTTCTGGATCAGTCGTGG	TGAGGTTGCGAATACGAGTG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.024937	4448	5929	1946-2075	1.000000	85.6
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	GGGCCCGATCCAATAACAAT	AAGCTAACGGATCGTGGAAC	20	20	50.0	50.0	58.0	58.0	5.7	0.0	0.010594	4448	5929	2210-2339	1.000000	85.5
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CCTTTCTGGATCAGTCGTGG	AAGCTAACGGATCGTGGAAC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.021706	4448	5929	2137-2266	1.000000	85.5
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	TGAATCAAACGAGGGATCCC	20	20	50.0	50.0	58.0	57.3	0.0	24.6	0.714678	9362	10319	1060-1070	1.000000	79.4
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	TGAATCAAACGAGGGATCCC	20	20	50.0	50.0	58.0	57.3	0.0	24.6	0.716066	9362	10319	1059-1069	1.000000	79.4
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	ACGGAAAGAGAGGGATTCGA	TGAATCAAACGAGGGATCCC	20	20	50.0	50.0	58.2	57.3	0.0	24.6	0.861444	9362	10319	1114-1124	1.000000	78.2
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	TGGAAACGGAAAGAGAGGGA	TGAATCAAACGAGGGATCCC	20	20	50.0	50.0	58.3	57.3	0.0	24.6	0.985551	9362	10319	1119-1129	1.000000	77.2
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	CAATCCGACGCTTTAGTCCA	GGATCCATAGAACGAATCGCA	20	21	50.0	47.6	58.0	58.0	0.0	7.0	1.031212	9362	10319	1028-1038	1.000000	76.9
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	GGTTCAAATCCTATTGGACGC	CATTCACTCGGGAAGCAGAA	21	20	47.6	50.0	57.3	57.9	0.0	0.0	1.791932	11569	12027	573-595	1.000000	67.6
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	GGTTCAAATCCTATTGGACGC	AAGACATTCACTCGGGAAGC	21	20	47.6	50.0	57.3	57.9	0.0	0.0	1.793395	11569	12027	577-599	1.000000	67.6
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	GGTTCAAATCCTATTGGACGC	GGAAGCAGAAGCCCTTTTGA	21	20	47.6	50.0	57.3	58.4	0.0	3.1	2.071968	11569	12027	563-585	1.000000	65.4
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	GGTTCAAATCCTATTGGACGC	ATTCACTCGGGAAGCAGAAG	21	20	47.6	50.0	57.3	57.6	0.0	0.0	2.086062	11569	12027	572-594	1.000000	65.2
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	TGGATAGGACAGAGGTCTTCT	CATTCACTCGGGAAGCAGAA	21	20	47.6	50.0	56.9	57.9	0.0	0.0	2.150746	11569	12027	608-630	1.000000	64.6
atpF-atpH_p1	atpF-atpH	1	ATTAAACCCGAAACTCCCGG	TGGGCTGGTTGTAGCATTAG	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.536681	14913	15546	752-802	1.000000	76.4
atpF-atpH_p2	atpF-atpH	2	ATTAAACCCGAAACTCCCGG	AGGCAGAGGGAAAAATACGG	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.538372	14913	15546	819-869	1.000000	76.4
atpF-atpH_p3	atpF-atpH	3	ATTAAACCCGAAACTCCCGG	GCAGAGGGAAAAATACGGGG	20	20	50.0	55.0	57.9	58.6	0.0	0.0	0.728758	14913	15546	817-867	1.000000	74.8
atpF-atpH_p4	atpF-atpH	4	ATTAAACCCGAAACTCCCGG	GGCAGAGGGAAAAATACGGG	20	20	50.0	55.0	57.9	58.6	0.0	0.0	0.728758	14913	15546	818-868	1.000000	74.8
atpF-atpH_p5	atpF-atpH	5	ATTAAACCCGAAACTCCCGG	CCGAGGCAGAGGGAAAAATA	20	20	50.0	50.0	57.9	57.3	0.0	0.0	0.828409	14913	15546	822-872	1.000000	74.0
atpH-atpI_p1	atpH-atpI	1	CCTTCTACAGCTTGACCTGC	TTTTGCAACTTTAGCTGCGG	20	20	55.0	45.0	58.3	57.9	0.0	0.0	0.401020	15793	17241	1617-1627	1.000000	77.9
atpH-atpI_p2	atpH-atpI	2	TCCCTTCTACAGCTTGACCT	TTTTGCAACTTTAGCTGCGG	20	20	50.0	45.0	57.7	57.9	0.0	0.0	0.460065	15793	17241	1619-1629	1.000000	77.5
atpH-atpI_p3	atpH-atpI	3	CCCTTCTACAGCTTGACCTG	TTTTGCAACTTTAGCTGCGG	20	20	55.0	45.0	57.6	57.9	0.0	0.0	0.526012	15793	17241	1618-1628	1.000000	76.9
atpH-atpI_p4	atpH-atpI	4	GCGATCCCTTCTACAGCTTG	TTTTGCAACTTTAGCTGCGG	20	20	55.0	45.0	58.4	57.9	0.0	0.0	0.556384	15793	17241	1623-1633	1.000000	76.7
atpH-atpI_p5	atpH-atpI	5	AGGTCCAATAGAAGCAAGCC	TTTTGCAACTTTAGCTGCGG	20	20	50.0	45.0	57.6	57.9	0.0	0.0	0.559583	15793	17241	1576-1586	1.000000	76.7
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	ACATTCCCGCATTTACACCC	CTATTTTGTCGACCAGGCGA	20	20	50.0	50.0	58.5	58.0	0.0	14.1	0.534532	29605	30809	1307-1318	1.000000	77.3
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	ACATTCCCGCATTTACACCC	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.612077	29605	30809	1284-1295	1.000000	76.7
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	ACATTCCCGCATTTACACCC	AGCTATTTTGTCGACCAGGC	20	20	50.0	50.0	58.5	58.3	0.0	14.1	0.790882	29605	30809	1309-1320	1.000000	75.3
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	GCATTTACACCCCCAAGCAT	CTATTTTGTCGACCAGGCGA	20	20	50.0	50.0	58.8	58.0	0.0	14.1	0.810365	29605	30809	1299-1310	1.000000	75.1
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	GACATTCCCGCATTTACACC	CTATTTTGTCGACCAGGCGA	20	20	50.0	50.0	57.2	58.0	0.0	14.1	0.848705	29605	30809	1308-1319	1.000000	74.8
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	30881	32179	1355-1359	1.000000	80.1
trnC-GCA-petN_p2	trnC-GCA-petN	2	GTATCATTTTGGCGGCATGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	57.9	58.2	14.3	0.0	0.289944	30881	32179	1407-1411	1.000000	79.6
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.432990	30881	32179	1416-1420	1.000000	78.5
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTCCCCAGTTCAAATCCGG	TCTTCCCCATACTACGAGGG	20	20	50.0	55.0	58.1	57.6	0.0	0.0	0.458823	30881	32179	1406-1410	1.000000	78.3
trnC-GCA-petN_p5	trnC-GCA-petN	5	GTATCATTTTGGCGGCATGG	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	57.9	57.6	14.3	0.0	0.489459	30881	32179	1468-1472	1.000000	77.9
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	CTTCTCCTGATGGTTGGTCG	GCGTCTTCTCTTTGGCAAAG	20	20	55.0	50.0	58.0	57.7	0.0	29.6	0.338107	40895	41826	931-1087	1.000000	82.1
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	CTTCTCCTGATGGTTGGTCG	ATACATAAATAGGGGGCGCG	20	20	55.0	50.0	58.0	57.6	0.0	0.0	0.412325	40895	41826	975-1131	1.000000	81.5
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	TTGCTTCTCCTGATGGTTGG	GCGTCTTCTCTTTGGCAAAG	20	20	50.0	50.0	57.8	57.7	0.0	29.6	0.527394	40895	41826	934-1090	1.000000	80.6
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	TCCTGATGGTTGGTCGAGTA	GCGTCTTCTCTTTGGCAAAG	20	20	50.0	50.0	57.8	57.7	0.0	29.6	0.547753	40895	41826	927-1083	1.000000	80.4
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	GCTTCTCCTGATGGTTGGTC	GCGTCTTCTCTTTGGCAAAG	20	20	55.0	50.0	58.3	57.7	0.0	29.6	0.578376	40895	41826	932-1088	1.000000	80.2
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	51716	52902	1259-1269	1.000000	82.2
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	51716	52902	1267-1277	1.000000	81.7
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	AAATGCGACGCTCTAACCTC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.4	58.0	22.1	0.0	0.363047	51716	52902	1231-1241	1.000000	81.4
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	AAATGCGACGCTCTAACCTC	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.4	58.1	22.1	0.0	0.427856	51716	52902	1239-1249	1.000000	80.8
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	AGTCCGTAGCGTCTACCAAT	20	20	50.0	50.0	57.8	58.2	15.5	10.2	0.480302	51716	52902	1273-1283	1.000000	80.3
trnM-CAU-atpE_p1	trnM-CAU-atpE	1	TATTGCTTTCATACGGCGGG	CTCTCAGACGAGCTAGGACA	20	20	50.0	55.0	58.4	58.0	13.2	0.0	0.442584	58123	58398	375-375	1.000000	77.7
trnM-CAU-atpE_p2	trnM-CAU-atpE	2	TATTGCTTTCATACGGCGGG	CGAGCTAGGACACGAGTAGA	20	20	50.0	55.0	58.4	58.1	13.2	0.0	0.474048	58123	58398	367-367	1.000000	77.5
trnM-CAU-atpE_p3	trnM-CAU-atpE	3	TATTGCTTTCATACGGCGGG	TCTCAGACGAGCTAGGACAC	20	20	50.0	55.0	58.4	58.3	13.2	0.0	0.671029	58123	58398	374-374	1.000000	75.9
trnM-CAU-atpE_p4	trnM-CAU-atpE	4	GTATTGCTTTCATACGGCGG	CTCTCAGACGAGCTAGGACA	20	20	50.0	55.0	57.3	58.0	13.2	0.0	0.694537	58123	58398	376-376	1.000000	75.7
trnM-CAU-atpE_p5	trnM-CAU-atpE	5	GTATTGCTTTCATACGGCGG	CGAGCTAGGACACGAGTAGA	20	20	50.0	55.0	57.3	58.1	13.2	0.0	0.726001	58123	58398	368-368	1.000000	75.5
rbcL-accD_p1	rbcL-accD	1	AGTCCTGAATTAGCTGCTGC	ACTTAGCCCACACCTGTATTC	20	21	50.0	47.6	58.0	57.4	13.4	0.0	1.664672	62513	63540	1116-1169	1.000000	72.2
rbcL-accD_p2	rbcL-accD	2	AGTCCTGAATTAGCTGCTGC	AGCCCACACCTGTATTCTAAC	20	21	50.0	47.6	58.0	57.4	13.4	0.0	1.664672	62513	63540	1112-1165	1.000000	72.2
rbcL-accD_p3	rbcL-accD	3	AGTCCTGAATTAGCTGCTGC	GCCCACACCTGTATTCTAACT	20	21	50.0	47.6	58.0	57.4	13.4	0.0	1.664672	62513	63540	1111-1164	1.000000	72.2
rbcL-accD_p4	rbcL-accD	4	GAGGCTAGCAAATGGAGTCC	AGCCCACACCTGTATTCTAAC	20	21	55.0	47.6	58.0	57.4	18.2	0.0	1.668725	62513	63540	1127-1180	1.000000	72.1
rbcL-accD_p5	rbcL-accD	5	GAGGCTAGCAAATGGAGTCC	GCCCACACCTGTATTCTAACT	20	21	55.0	47.6	58.0	57.4	18.2	0.0	1.668725	62513	63540	1126-1179	1.000000	72.1
petA-psbJ_p1	petA-psbJ	1	AGAAACAGTTTGAGAAGGTTCA	ATTCCGGATTGGGTTCATCC	22	20	36.4	50.0	55.8	57.6	2.3	4.1	4.578928	68801	70083	1353-1389	1.000000	44.3
petA-psbJ_p2	petA-psbJ	2	AAGAAACAGTTTGAGAAGGTTCA	ATTCCGGATTGGGTTCATCC	23	20	34.8	50.0	56.5	57.6	2.3	4.1	4.859044	68801	70083	1354-1390	1.000000	42.1
petA-psbJ_p3	petA-psbJ	3	AGAAACAGTTTGAGAAGGTTCAA	ATTCCGGATTGGGTTCATCC	23	20	34.8	50.0	56.5	57.6	2.3	4.1	4.859044	68801	70083	1353-1389	1.000000	42.1
petA-psbJ_p4	petA-psbJ	4	GAAACAGTTTGAGAAGGTTCA	ATTCCGGATTGGGTTCATCC	21	20	38.1	50.0	54.3	57.6	2.3	4.1	5.100398	68801	70083	1352-1388	1.000000	40.9
petA-psbJ_p5	petA-psbJ	5	AAGAAACAGTTTGAGAAGGTTCAA	ATTCCGGATTGGGTTCATCC	24	20	33.3	50.0	57.2	57.6	2.3	4.1	5.199141	68801	70083	1354-1390	1.000000	40.9
rpl33-rps18_p1	rpl33-rps18	1	TCTGTCCCTGTTGTTGCAAA	TCAATTCGATCCCCCGATTG	20	20	45.0	50.0	57.9	57.7	0.0	0.0	0.390844	74116	74532	507-553	1.000000	81.3
rpl33-rps18_p2	rpl33-rps18	2	TTCTGTCCCTGTTGTTGCAA	TCAATTCGATCCCCCGATTG	20	20	45.0	50.0	57.9	57.7	0.0	0.0	0.390844	74116	74532	508-554	1.000000	81.3
rpl33-rps18_p3	rpl33-rps18	3	ATTCTGTCCCTGTTGTTGCA	TCAATTCGATCCCCCGATTG	20	20	45.0	50.0	57.6	57.7	0.0	0.0	0.617852	74116	74532	509-555	1.000000	79.5
rpl33-rps18_p4	rpl33-rps18	4	TCTGTCCCTGTTGTTGCAAA	GGGCAAACGTCTACGAAAAG	20	20	45.0	50.0	57.9	57.4	0.0	9.9	0.725636	74116	74532	481-527	1.000000	78.7
rpl33-rps18_p5	rpl33-rps18	5	TTCTGTCCCTGTTGTTGCAA	GGGCAAACGTCTACGAAAAG	20	20	45.0	50.0	57.9	57.4	0.0	9.9	0.725636	74116	74532	482-528	1.000000	78.7
rps3-rps19_p1	rps3-rps19	1	TACCAAGTCTGAAACCGAGC	ATAAACTTCCGGGGACATGC	20	20	50.0	50.0	57.5	57.9	0.0	0.0	0.503392	90490	90915	512-514	1.000000	79.6
rps3-rps19_p2	rps3-rps19	2	GTCTGAAACCGAGCGGATTT	ATAAACTTCCGGGGACATGC	20	20	50.0	50.0	58.6	57.9	16.8	0.0	0.613103	90490	90915	506-508	1.000000	78.7
rps3-rps19_p3	rps3-rps19	3	TGGTTGTGCAAACCAAAGAG	ATAAACTTCCGGGGACATGC	20	20	45.0	50.0	57.0	57.9	22.9	0.0	1.010273	90490	90915	550-552	1.000000	75.3
rps3-rps19_p4	rps3-rps19	4	TCTGAAACCGAGCGGATTTA	ATAAACTTCCGGGGACATGC	20	20	45.0	50.0	57.0	57.9	16.8	0.0	1.095356	90490	90915	505-507	1.000000	74.9
rps3-rps19_p5	rps3-rps19	5	GTCTGAAACCGAGCGGATT	ATAAACTTCCGGGGACATGC	19	20	52.6	50.0	57.9	57.9	16.8	0.0	1.191811	90490	90915	506-508	1.000000	74.1
rps19_p1	rps19	1	TTGTCCCATAATCCCCCACT	GATGAAGGAACGGGAAGACC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.031190	90916	91194	1433-1433	0.500000	59.8
rps19_p2	rps19	2	TTGTCCCATAATCCCCCACT	CCAGATGAAGGAACGGGAAG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.035440	90916	91194	1436-1436	0.500000	59.7
rps19_p3	rps19	3	TTGTCCCATAATCCCCCACT	GGGCCCCAATTGGTAGAAAA	20	20	50.0	50.0	58.0	58.1	0.0	22.9	0.060021	90916	91194	895-895	0.500000	59.5
rps19_p4	rps19	4	TTATCCGTGCAATTCCCTCG	GATGAAGGAACGGGAAGACC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.077484	90916	91194	1614-1614	0.500000	59.4
rps19_p5	rps19	5	TTATCCGTGCAATTCCCTCG	CCAGATGAAGGAACGGGAAG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.081734	90916	91194	1617-1617	0.500000	59.3
ndhE_p1	ndhE	1	CGTTTTCGCAAACATGATCA	CGAAATTGATAAGGAGTTGGTCA	20	23	40.0	39.1	55.6	56.9	11.2	0.0	6.532464	124555	124860	428-428	1.000000	44.0
ndhE_p2	ndhE	2	TCGCAAACATGATCATACGT	CGAAATTGATAAGGAGTTGGTCA	20	23	40.0	39.1	55.6	56.9	8.1	0.0	6.553194	124555	124860	423-423	1.000000	44.0
ndhE_p3	ndhE	3	CGTTTTCGCAAACATGATCA	TCGAAATTGATAAGGAGTTGGTCA	20	24	40.0	37.5	55.6	58.2	11.2	0.0	6.600951	124555	124860	429-429	1.000000	44.0
ndhE_p4	ndhE	4	TCGCAAACATGATCATACGT	TCGAAATTGATAAGGAGTTGGTCA	20	24	40.0	37.5	55.6	58.2	8.1	0.0	6.621681	124555	124860	424-424	1.000000	44.0
ndhE_p5	ndhE	5	TCGCAAACATGATCATACGTT	CGAAATTGATAAGGAGTTGGTCA	21	23	38.1	39.1	56.4	56.9	8.1	0.0	6.776686	124555	124860	423-423	1.000000	44.0
ndhA_p1	ndhA	1	GATTTGCGTGGGCTAAGGTA	GTGAGAGTTGAAGCCCCAAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.051424	126428	128956	2911-2913	1.000000	80.7
ndhA_p2	ndhA	2	TTGCGTGGGCTAAGGTAATC	GTGAGAGTTGAAGCCCCAAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.051424	126428	128956	2908-2910	1.000000	80.7
ndhA_p3	ndhA	3	GATTTGCGTGGGCTAAGGTA	AAAAAGCCGTCTCCGACTTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.069508	126428	128956	2956-2958	1.000000	80.6
ndhA_p4	ndhA	4	TTGCGTGGGCTAAGGTAATC	AAAAAGCCGTCTCCGACTTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.069508	126428	128956	2953-2955	1.000000	80.6
ndhA_p5	ndhA	5	TGCAGCTCGTACTGTTTGTT	GTGAGAGTTGAAGCCCCAAA	20	20	45.0	50.0	58.1	58.0	8.8	0.0	0.070639	126428	128956	2865-2867	1.000000	80.6