primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAGCCGAGTACTCTACCGTT	AGAGGGCCGAGGTTTTTAAA	20	20	50.0	45.0	58.2	57.0	20.3	0.0	1.132019	4295	4990	767-810	1.000000	78.6
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	AGGGCCGAGGTTTTTAAAGA	20	20	50.0	45.0	58.2	57.0	20.3	0.0	1.132019	4295	4990	765-808	1.000000	78.6
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAGCCGAGTACTCTACCGTT	AAAGAGGGCCGAGGTTTTTA	20	20	50.0	45.0	58.2	57.0	20.3	0.0	1.132019	4295	4990	769-812	1.000000	78.5
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	AAGAGGGCCGAGGTTTTTAA	20	20	50.0	45.0	58.2	57.0	20.3	0.0	1.132019	4295	4990	768-811	1.000000	78.5
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	TAAAGAGGGCCGAGGTTTTT	20	20	50.0	45.0	58.2	57.0	20.3	0.0	1.132019	4295	4990	770-813	1.000000	78.5
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6127	6656	603-628	1.000000	84.0
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	6127	6656	614-639	1.000000	83.4
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	TAAGGCATCGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.2	8.1	0.0	0.310345	6127	6656	633-658	1.000000	83.0
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCATGTCCTTCAAGTCGCAC	TAAGGCATCGGGTTTTGGTC	20	20	50.0	50.0	58.2	58.2	0.0	0.0	0.375253	6127	6656	644-669	1.000000	82.4
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	GTAAGGCATCGGGTTTTGGT	20	20	45.0	50.0	58.1	58.5	8.1	0.0	0.597945	6127	6656	634-659	1.000000	80.7
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CTTTCGTCCACTCAGCCATC	GGGTTAGTAGAACGAATCGCA	20	21	55.0	47.6	58.6	57.8	0.0	1.2	1.791349	7999	8811	834-883	1.000000	76.0
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	CTTTCGTCCACTCAGCCATC	ACGAATCGCACTTTTACCACT	20	21	55.0	42.9	58.6	58.2	0.0	1.2	1.873511	7999	8811	823-872	1.000000	75.4
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CTTTCGTCCACTCAGCCATC	ACGAATCGCACTTTTACCAC	20	20	55.0	45.0	58.6	56.8	0.0	1.2	1.880537	7999	8811	823-872	1.000000	75.4
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CTTTCGTCCACTCAGCCATC	AACGAATCGCACTTTTACCAC	20	21	55.0	42.9	58.6	57.5	0.0	1.2	2.167519	7999	8811	824-873	1.000000	73.1
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	CTTTCGTCCACTCAGCCATC	GGGGTTAGTAGAACGAATCGC	20	21	55.0	52.4	58.6	58.5	0.0	1.2	2.168419	7999	8811	835-884	1.000000	73.0
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGAATGAAGAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	55.2	58.5	0.0	5.4	3.295722	29049	29780	843-1312	1.000000	58.0
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	ACTAGAATGAAGAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	23	20	43.5	50.0	58.0	58.5	0.0	5.4	3.482253	29049	29780	846-1315	1.000000	56.5
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AGGAACTAGAATGAAGAGTGCAG	TTCAATTGGTCAGAGCACCG	23	20	43.5	50.0	57.9	58.5	0.0	5.4	3.560865	29049	29780	850-1319	1.000000	55.9
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AGGAACTAGAATGAAGAGTGCA	TTCAATTGGTCAGAGCACCG	22	20	40.9	50.0	56.8	58.5	0.0	5.4	3.643922	29049	29780	850-1319	1.000000	55.2
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	GGAACTAGAATGAAGAGTGCAGT	TTCAATTGGTCAGAGCACCG	23	20	43.5	50.0	58.2	58.5	0.0	5.4	3.650775	29049	29780	849-1318	1.000000	55.2
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	30436	31167	766-835	1.000000	87.3
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	30436	31167	745-814	1.000000	87.3
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	GCTGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	58.3	58.1	0.0	0.0	0.327456	30436	31167	745-814	1.000000	87.0
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.7	58.1	0.0	0.0	0.345606	30436	31167	724-793	1.000000	87.0
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	GCTGCCTCCTTGAAAGAGAG	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	58.3	58.1	0.0	0.0	0.349611	30436	31167	742-811	1.000000	86.9
psaA-ycf3_p1	psaA-ycf3	1	GGTTTGGCCCATTCCTCAAA	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	58.7	58.0	0.0	0.0	0.693896	41484	42369	1070-1115	1.000000	79.1
psaA-ycf3_p2	psaA-ycf3	2	TTTTGACTTCTGGTTCCGGC	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	58.7	58.0	0.0	0.0	0.726228	41484	42369	1018-1063	1.000000	79.1
psaA-ycf3_p3	psaA-ycf3	3	TGGCCCATTCCTCAAAAGAA	ATCAAGCCGCTGAGTATTGG	20	20	45.0	50.0	57.3	58.0	0.0	0.0	0.733667	41484	42369	1066-1111	1.000000	78.8
psaA-ycf3_p4	psaA-ycf3	4	TTGGCCCATTCCTCAAAAGA	ATCAAGCCGCTGAGTATTGG	20	20	45.0	50.0	57.3	58.0	0.0	0.0	0.733667	41484	42369	1067-1112	1.000000	78.8
psaA-ycf3_p5	psaA-ycf3	5	GGTTTGGCCCATTCCTCAAA	CGATAACGCTTACTCCTGGG	20	20	50.0	55.0	58.7	57.9	0.0	0.0	0.788602	41484	42369	1043-1088	1.000000	78.5
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	21	20	47.6	50.0	58.0	58.1	8.2	0.0	1.134847	44391	44842	541-759	1.000000	77.1
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTCAA	21	20	47.6	45.0	58.0	57.8	8.2	0.0	1.233148	44391	44842	512-730	1.000000	76.6
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	21	20	47.6	50.0	58.0	58.2	8.2	0.0	1.191833	44391	44842	562-780	1.000000	76.4
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTC	21	20	47.6	50.0	58.0	58.3	8.2	0.0	1.301418	44391	44842	514-732	1.000000	76.0
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TGGCCACAATTGAAAAGGTC	CCCTCGGTAAACAAAAGCCT	20	20	45.0	50.0	56.8	58.1	13.6	0.0	1.291736	44391	44842	577-795	1.000000	75.5
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TGGTTCAGGCATTTGCTCAT	GTCAGAGCACATGGAACCAT	20	20	45.0	50.0	58.1	57.9	2.1	0.0	0.192314	50573	51711	2027-2055	1.000000	84.5
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TGGTTCAGGCATTTGCTCAT	TTCAGGTGCTCGTTTAGTCG	20	20	45.0	50.0	58.1	57.9	2.1	0.0	0.132838	50573	51711	2328-2351	1.000000	84.4
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CAATGCTCCTTTTCGCCATG	TTCAGGTGCTCGTTTAGTCG	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.143053	50573	51711	1983-1983	0.500000	58.9
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TGGTTCAGGCATTTGCTCAT	AGTTCGGTTCGATTCAGGTG	20	20	45.0	50.0	58.1	57.9	2.1	0.0	0.152799	50573	51711	2363-2363	0.500000	58.8
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CAATGCTCCTTTTCGCCATG	AGTTCGGTTCGATTCAGGTG	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.163013	50573	51711	1995-1995	0.500000	58.7
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAAAAACCCG	20	20	50.0	50.0	57.9	58.6	6.4	0.0	0.605464	65302	66480	1326-1335	1.000000	79.2
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	GCCGCTAGTAAAAACCCGAA	20	20	50.0	50.0	57.9	58.6	6.4	0.0	0.609597	65302	66480	1324-1333	1.000000	79.2
psbE-petL_p3	psbE-petL	3	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAAAAACCCG	20	20	50.0	50.0	57.8	58.6	24.9	0.0	0.710472	65302	66480	1249-1258	1.000000	78.7
psbE-petL_p4	psbE-petL	4	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAAAAACCCGAA	20	20	50.0	50.0	57.8	58.6	24.9	0.0	0.714605	65302	66480	1247-1256	1.000000	78.6
psbE-petL_p5	psbE-petL	5	ACGAATAACCAACCCGCAAT	AAGCCGCTAGTAAAAACCCG	20	20	45.0	50.0	58.2	58.6	0.0	0.0	0.725150	65302	66480	1335-1344	1.000000	78.3
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	ACCCGTGACATTTTGTACCC	GGTGCCACGGAAAGATATGT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.138141	67333	67739	483-497	1.000000	86.9
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CCCGTGACATTTTGTACCCA	GGTGCCACGGAAAGATATGT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.139485	67333	67739	482-496	1.000000	86.9
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	ACAGGATTTGAACCCGTGAC	GGTGCCACGGAAAGATATGT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.146177	67333	67739	494-508	1.000000	86.7
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	CAGGATTTGAACCCGTGACA	GGTGCCACGGAAAGATATGT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.147511	67333	67739	493-507	1.000000	86.7
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	TGACAGGATTTGAACCCGTG	GGTGCCACGGAAAGATATGT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.147511	67333	67739	496-510	1.000000	86.6
clpP_p1	clpP	1	TAACGTTCGCTACCAATGGG	AGTAGCAATACGCAATGGGG	20	20	50.0	50.0	58.0	58.0	2.8	1.6	0.045618	70891	72990	2809-2981	1.000000	90.5
clpP_p2	clpP	2	TAACGTTCGCTACCAATGGG	GTAGCAATACGCAATGGGGA	20	20	50.0	50.0	58.0	58.0	2.8	1.6	0.051143	70891	72990	2808-2980	1.000000	90.4
clpP_p3	clpP	3	CTAGTACATGTTCCTCGGCG	AGTAGCAATACGCAATGGGG	20	20	55.0	50.0	57.9	58.0	2.3	1.6	0.153798	70891	72990	2577-2744	1.000000	89.9
clpP_p4	clpP	4	CTAGTACATGTTCCTCGGCG	GTAGCAATACGCAATGGGGA	20	20	55.0	50.0	57.9	58.0	2.3	1.6	0.159323	70891	72990	2576-2743	1.000000	89.8
clpP_p5	clpP	5	TAACGTTCGCTACCAATGGG	ACGCAATGGGGAGTTGATAC	20	20	50.0	50.0	58.0	58.0	2.8	1.6	0.051324	70891	72990	2972-2972	0.500000	59.6
rpl22_p1	rpl22	1	CAAGTCGGAAACCAAGTGGA	TGTTCATTAATGGGAGGTAAACT	20	23	50.0	34.8	58.0	55.6	3.8	15.4	5.486391	84493	85122	633-676	1.000000	52.0
rpl22_p2	rpl22	2	ACCAAGTCGGAAACCAAGTG	TGTTCATTAATGGGAGGTAAACT	20	23	50.0	34.8	58.3	55.6	0.0	15.4	5.772633	84493	85122	635-678	1.000000	52.0
rpl22_p3	rpl22	3	CAAGTCGGAAACCAAGTGGA	TGTTCATTAATGGGAGGTAAACTT	20	24	50.0	33.3	58.0	56.3	3.8	15.4	5.776916	84493	85122	633-676	1.000000	52.0
rpl22_p4	rpl22	4	CAAGTCGGAAACCAAGTGGA	TTGTTCATTAATGGGAGGTAAACT	20	24	50.0	33.3	58.0	56.3	3.8	15.4	5.776916	84493	85122	634-677	1.000000	52.0
rpl22_p5	rpl22	5	CAAGTCGGAAACCAAGTGGA	TGTTCATTAATGGGAGGTAAAC	20	22	50.0	36.4	58.0	54.1	3.8	15.4	5.968612	84493	85122	633-676	1.000000	52.0
ndhF-rpl32_p1	ndhF-rpl32	1	GCCAAAATCCCCTACACGAT	AACGCTGCCAAATATCCCTT	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.196658	113766	114469	1372-1372	0.500000	58.4
ndhF-rpl32_p2	ndhF-rpl32	2	GCCAAAATCCCCTACACGAT	ACGCTGCCAAATATCCCTTT	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.196658	113766	114469	1371-1371	0.500000	58.4
ndhF-rpl32_p3	ndhF-rpl32	3	GCCAAAATCCCCTACACGAT	GGCCGATTCCTGTTCTTCAA	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.231566	113766	114469	1466-1466	0.500000	58.1
ndhF-rpl32_p4	ndhF-rpl32	4	GCCCATATACGCCGAAGATT	AACGCTGCCAAATATCCCTT	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.238638	113766	114469	930-930	0.500000	58.1
ndhF-rpl32_p5	ndhF-rpl32	5	GCCCATATACGCCGAAGATT	ACGCTGCCAAATATCCCTTT	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.238638	113766	114469	929-929	0.500000	58.1
ycf1_p1	ycf1	1	GCTCTTCCACTCGTCGAAAT	AAAGAAACGGAAGAAGGCGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.039159	128498	128617	714-714	0.500000	59.7
ycf1_p2	ycf1	2	ATCTTCCGACCATTCTGCAC	AAAGAAACGGAAGAAGGCGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.066666	128498	128617	696-696	0.500000	59.5
ycf1_p3	ycf1	3	GCTCTTCCACTCGTCGAAAT	AGAAACGGAAGAAGGCGTAC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.078310	128498	128617	712-712	0.500000	59.4
ycf1_p4	ycf1	4	ATCTTCCGACCATTCTGCAC	AGAAACGGAAGAAGGCGTAC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.105817	128498	128617	694-694	0.500000	59.2
ycf1_p5	ycf1	5	GCTCTTCCACTCGTCGAAAT	TTGGGGGTTTAAGCTTCTCG	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.196532	128498	128617	448-448	0.500000	58.4