primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	GTGCTCAACCCACAGGAAC	20	19	50.0	57.9	58.2	58.7	20.3	0.0	1.837155	4534	5405	863-1273	0.985075	76.7
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	GTGCTCAACCCACAGGAAC	20	19	50.0	57.9	58.2	58.7	20.3	0.0	1.837155	4534	5405	862-1272	0.985075	76.7
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAAGCCGAGTACTCTACCGT	TGCTCAACCCACAGGAACT	20	19	50.0	52.6	58.2	58.8	20.3	0.0	1.937756	4534	5405	862-1272	1.000000	76.5
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	TGCTCAACCCACAGGAACT	20	19	50.0	52.6	58.2	58.8	20.3	0.0	1.937756	4534	5405	861-1271	1.000000	76.5
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TACCGTTGAGTTAGCAACCC	GTGCTCAACCCACAGGAAC	20	19	50.0	57.9	57.5	58.7	0.0	0.0	2.129492	4534	5405	849-1259	0.985075	74.4
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	AATGGAAAGACCGCTTAACT	20	20	50.0	40.0	58.0	55.0	0.0	0.0	3.047468	8433	9408	1083-1597	1.000000	67.6
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	AATGGAAAGACCGCTTAACT	20	20	50.0	40.0	58.0	55.0	0.0	0.0	3.048856	8433	9408	1082-1596	1.000000	67.6
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CTTTAGTCCACTCAGCCACC	AATGGAAAGACCGCTTAACT	20	20	55.0	40.0	57.9	55.0	0.0	0.0	3.143490	8433	9408	1073-1587	1.000000	66.9
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CAATCCGACGCTTTAGTCCA	TGGAAAGACCGCTTAACTATTGA	20	23	50.0	39.1	58.0	57.8	0.0	0.0	3.159861	8433	9408	1081-1595	0.985075	66.1
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	AATCCGACGCTTTAGTCCAC	TGGAAAGACCGCTTAACTATTGA	20	23	50.0	39.1	58.0	57.8	0.0	0.0	3.161249	8433	9408	1080-1594	0.985075	66.1
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	GGTTCAAATCCTATTGGACGC	CACTGAAGAAGCTGAAACCCT	21	21	47.6	47.6	57.3	58.1	0.0	0.0	2.836569	10371	10609	280-542	1.000000	69.3
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	GGTTCAAATCCTATTGGACGC	TCACTGAAGAAGCTGAAACCC	21	21	47.6	47.6	57.3	58.1	0.0	0.0	2.841596	10371	10609	281-543	1.000000	69.3
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	GGTTCAAATCCTATTGGACGC	ACTGAAGAAGCTGAAACCCT	21	20	47.6	45.0	57.3	56.7	0.0	0.0	3.027201	10371	10609	279-541	1.000000	67.8
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	GGTTCAAATCCTATTGGACGC	CACTGAAGAAGCTGAAACCC	21	20	47.6	50.0	57.3	56.6	0.0	0.0	3.052985	10371	10609	280-542	1.000000	67.6
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	GGGCGCCCATTGTCTAATG	CACTGAAGAAGCTGAAACCCT	19	21	57.9	47.6	59.0	58.1	40.7	0.0	3.110797	10371	10609	332-594	1.000000	67.1
trnC-GCA-petN_p1	trnC-GCA-petN	1	AGTTCAAATCTGGGTGTCGC	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.5	58.2	0.0	0.0	0.627590	28233	29016	681-1205	1.000000	87.0
trnC-GCA-petN_p2	trnC-GCA-petN	2	GGGGGACTGCAAATCCTTTA	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	57.5	58.2	14.7	0.0	0.676453	28233	29016	706-1230	1.000000	86.6
trnC-GCA-petN_p3	trnC-GCA-petN	3	GACATGGCCAAGTGGTAAGG	AGCCCAAGCGAGACTTACTA	20	20	55.0	50.0	58.5	58.2	20.2	0.0	0.687674	28233	29016	727-1251	1.000000	86.5
trnC-GCA-petN_p4	trnC-GCA-petN	4	AGTTCAAATCTGGGTGTCGC	CCCACTCCTTCCCCATACTA	20	20	50.0	55.0	58.5	57.5	0.0	0.0	0.965770	28233	29016	738-1262	1.000000	84.3
trnC-GCA-petN_p5	trnC-GCA-petN	5	GGGGGACTGCAAATCCTTTA	CCCACTCCTTCCCCATACTA	20	20	50.0	55.0	57.5	57.5	14.7	0.0	1.014633	28233	29016	763-1287	1.000000	83.9
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	CGTTTATTGCTACTGCGCTG	20	20	50.0	50.0	58.2	57.9	0.0	13.2	0.228503	29107	30285	617-1858	0.985075	89.6
petN-psbM_p2	petN-psbM	2	TAGTAAGTCTCGCTTGGGCT	ATTGCTACTGCGCTGTTCAT	20	20	50.0	45.0	58.2	58.3	0.0	25.3	0.408587	29107	30285	612-1853	1.000000	88.7
petN-psbM_p3	petN-psbM	3	TAGTAAGTCTCGCTTGGGCT	TATTGCTACTGCGCTGTTCA	20	20	50.0	45.0	58.2	57.6	0.0	25.3	0.529793	29107	30285	613-1854	1.000000	87.8
petN-psbM_p4	petN-psbM	4	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCGCTGTTCATTT	20	20	50.0	45.0	58.2	58.5	0.0	25.3	0.628680	29107	30285	610-1851	1.000000	87.0
petN-psbM_p5	petN-psbM	5	TAGTATGGGGAAGGAGTGGG	CGTTTATTGCTACTGCGCTG	20	20	55.0	50.0	57.5	57.9	0.0	13.2	0.566683	29107	30285	573-1801	0.970149	86.3
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.175803	31869	33372	584-2598	0.970149	89.4
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.273369	31869	33372	549-2563	0.970149	88.6
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	31869	33372	562-2576	0.970149	88.5
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	20	20	50.0	55.0	57.8	58.2	0.0	0.0	0.320736	31869	33372	581-2595	0.970149	88.2
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	CGTTGCCTCCTTGAAAGAGA	TTTTCCGCCGAAAAACGAAG	20	20	50.0	45.0	57.8	57.9	0.0	10.0	0.278010	31869	33372	646-2660	0.940299	87.4
psaA-ycf3_p1	psaA-ycf3	1	CCCATTCCTCGAAAGACGTT	CAGAGGCTTGGTTCGATCAA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.193903	43136	43775	642-861	1.000000	90.3
psaA-ycf3_p2	psaA-ycf3	2	CCCATTCCTCGAAAGACGTT	ATAGCGCTTACTCCAGGGAA	20	20	50.0	50.0	57.9	58.2	0.0	18.7	0.305239	43136	43775	598-817	1.000000	89.6
psaA-ycf3_p3	psaA-ycf3	3	CCCATTCCTCGAAAGACGTT	TAGCGCTTACTCCAGGGAAT	20	20	50.0	50.0	57.9	58.2	0.0	18.7	0.305239	43136	43775	597-816	1.000000	89.6
psaA-ycf3_p4	psaA-ycf3	4	CCCATTCCTCGAAAGACGTT	GTTCGATCAAGCTGCTGAGT	20	20	50.0	50.0	57.9	58.3	0.0	1.4	0.375163	43136	43775	632-851	1.000000	88.9
psaA-ycf3_p5	psaA-ycf3	5	CCCATTCCTCGAAAGACGTT	GCTATAGCGCTTACTCCAGG	20	20	50.0	55.0	57.9	57.6	0.0	18.7	0.450479	43136	43775	601-820	1.000000	88.4
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	AGAAAAAGAGCCGGCTATCG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.1	58.0	33.0	0.0	0.063299	47757	48840	821-1797	1.000000	91.5
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	AGAAAAAGAGCCGGCTATCG	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.1	58.1	33.0	0.0	0.128108	47757	48840	829-1805	1.000000	91.0
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	47757	48840	770-1746	1.000000	90.9
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	47757	48840	778-1754	1.000000	90.4
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	47757	48840	802-1778	1.000000	90.0
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	AAAACAAACGCGCTACCAAG	TAGACCCGCTAAAGACCCAA	20	20	45.0	50.0	57.9	58.1	5.7	0.0	0.172533	66682	67442	477-1337	1.000000	90.6
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	TGAACCCGCGACATTTTGTA	TAGACCCGCTAAAGACCCAA	20	20	45.0	50.0	58.1	58.1	8.2	0.0	0.178352	66682	67442	500-1360	1.000000	90.6
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	TTTGAACCCGCGACATTTTG	TAGACCCGCTAAAGACCCAA	20	20	45.0	50.0	57.9	58.1	8.2	0.0	0.185536	66682	67442	502-1362	1.000000	90.5
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	AGGGATGGCAGGATTTGAAC	TAGACCCGCTAAAGACCCAA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.208596	66682	67442	515-1375	1.000000	90.3
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	CAAAACAAACGCGCTACCAA	TAGACCCGCTAAAGACCCAA	20	20	45.0	50.0	58.2	58.1	5.7	0.0	0.229791	66682	67442	478-1338	1.000000	90.2
rpl33-rps18_p1	rpl33-rps18	1	TGATTCATGGGGAAATAAAGA	TAATCGATTCGATCCCCCGA	21	20	33.3	50.0	52.4	58.1	0.0	21.0	6.645142	68266	68621	335-680	0.985075	51.4
rpl33-rps18_p2	rpl33-rps18	2	TGATTCATGGGGAAATAAAGAA	TAATCGATTCGATCCCCCGA	22	20	31.8	50.0	53.4	58.1	0.0	21.0	6.726096	68266	68621	335-680	0.985075	51.4
rpl33-rps18_p3	rpl33-rps18	3	TGATTCATGGGGAAATAAAGA	GGGGTAAACGCCTACGAAAA	21	20	33.3	50.0	52.4	58.2	0.0	8.8	6.751780	68266	68621	307-652	0.985075	51.4
rpl33-rps18_p4	rpl33-rps18	4	TGATTCATGGGGAAATAAAGAA	GGGGTAAACGCCTACGAAAA	22	20	31.8	50.0	53.4	58.2	0.0	8.8	6.832735	68266	68621	307-652	0.985075	51.4
rpl33-rps18_p5	rpl33-rps18	5	TGATTCATGGGGAAATAAAGAAA	TAATCGATTCGATCCCCCGA	23	20	30.4	50.0	54.2	58.1	0.0	21.0	6.888171	68266	68621	335-680	0.985075	51.4
psbB-psbT_p1	psbB-psbT	1	GCTCAAGTGGAATTTGGAGC	TGGAACTTTAGGTGGTTCTCG	20	21	50.0	47.6	57.4	57.6	0.0	0.0	2.040194	75424	75850	376-834	0.985075	75.1
psbB-psbT_p2	psbB-psbT	2	GCTCAAGTGGAATTTGGAGC	GGAACTTTAGGTGGTTCTCGA	20	21	50.0	47.6	57.4	57.3	0.0	0.0	2.316700	75424	75850	375-833	0.985075	72.9
psbB-psbT_p3	psbB-psbT	3	TTTGCCGGTATTGATCCAGA	TGGAACTTTAGGTGGTTCTCG	20	21	45.0	47.6	56.9	57.6	1.9	0.0	2.487359	75424	75850	403-861	1.000000	72.1
psbB-psbT_p4	psbB-psbT	4	TGCCGGTATTGATCCAGATT	TGGAACTTTAGGTGGTTCTCG	20	21	45.0	47.6	56.7	57.6	1.9	0.0	2.719122	75424	75850	401-859	1.000000	70.2
psbB-psbT_p5	psbB-psbT	5	GCTCAAGTGGAATTTGGAGC	GGAACTTTAGGTGGTTCTCGAA	20	22	50.0	45.5	57.4	58.0	0.0	0.0	2.644887	75424	75850	375-833	0.985075	70.2
rps8-rpl14_p1	rps8-rpl14	1	CGGCAATAATGTCCCTACCC	TTGCTCGGGAATTGAGACAG	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.206932	82297	82743	485-739	0.985075	89.7
rps8-rpl14_p2	rps8-rpl14	2	CGGCAATAATGTCCCTACCC	GCTCGGGAATTGAGACAGTT	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.208395	82297	82743	483-737	0.985075	89.7
rps8-rpl14_p3	rps8-rpl14	3	CGGCAATAATGTCCCTACCC	CGATTGCTCGGGAATTGAGA	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.167816	82297	82743	488-742	0.970149	89.5
rps8-rpl14_p4	rps8-rpl14	4	CGGCAATAATGTCCCTACCC	ATTGCTCGGGAATTGAGACA	20	20	55.0	45.0	58.1	57.2	0.0	0.0	0.895263	82297	82743	486-740	0.985075	84.2
rps8-rpl14_p5	rps8-rpl14	5	TCGGCAATAATGTCCCTACC	CGATTGCTCGGGAATTGAGA	20	20	50.0	50.0	57.1	58.1	0.0	0.0	0.982765	82297	82743	489-743	0.970149	82.9
rpl16_p1	rpl16	1	CCAGAGATAAAGAAATCCGCG	AATTTAGGTTTCGTATAATTGCT	21	23	47.6	26.1	56.9	52.5	0.0	0.0	10.619347	83244	84896	1564-2167	0.955224	50.2
rpl16_p2	rpl16	2	CCAGAGATAAAGAAATCCGCG	CGATTTACTCTTTAATTTAGGTTTCGT	21	27	47.6	29.6	56.9	56.8	0.0	0.0	10.387074	83244	84896	1577-2180	0.895522	47.8
rpl16_p3	rpl16	3	TCCAGAGATAAAGAAATCCGCG	CGATTTACTCTTTAATTTAGGTTTCGT	22	27	45.5	29.6	58.3	56.8	0.0	0.0	10.532093	83244	84896	1578-2181	0.895522	47.8
rpl16_p4	rpl16	4	CCAGAGATAAAGAAATCCGCG	TCGATTTACTCTTTAATTTAGGTTTCG	21	27	47.6	29.6	56.9	56.5	0.0	0.0	10.600629	83244	84896	1578-2181	0.895522	47.8
rpl16_p5	rpl16	5	TCCAGAGATAAAGAAATCCGCG	TCGATTTACTCTTTAATTTAGGTTTCG	22	27	45.5	29.6	58.3	56.5	0.0	0.0	10.745648	83244	84896	1579-2182	0.895522	47.8
rps15-psaC_p1	rps15-psaC	1	TTCTGGGAAAACGTCAACGA	AATACATCCGTGGGACAAGC	20	20	45.0	50.0	57.7	58.0	9.9	0.0	0.351109	117420	118069	637-850	0.955224	87.4
rps15-psaC_p2	rps15-psaC	2	TTCTGGGAAAACGTCAACGA	GGGACAAGCTCGTACACATT	20	20	45.0	50.0	57.7	57.9	9.9	0.0	0.407485	117420	118069	626-839	0.955224	86.9
rps15-psaC_p3	rps15-psaC	3	TTCTGGGAAAACGTCAACGA	ACAAGCTCGTACACATTGGG	20	20	45.0	50.0	57.7	58.2	9.9	0.0	0.504312	117420	118069	623-836	0.955224	86.2
rps15-psaC_p4	rps15-psaC	4	TTCTGGGAAAACGTCAACGA	CAAGCTCGTACACATTGGGT	20	20	45.0	50.0	57.7	58.2	9.9	0.0	0.504312	117420	118069	622-835	0.955224	86.2
rps15-psaC_p5	rps15-psaC	5	TTCTGGGAAAACGTCAACGA	ACATCCGTCCCAAGGTATCA	20	20	45.0	50.0	57.7	58.1	9.9	0.0	0.438593	117420	118069	662-875	0.940299	85.6
ndhD-trnL-UAG_p1	ndhD-trnL-UAG	1	GATCTGTATCTTTCTACCTGTAA	TCCTAAGAGCAGCGTGTCTA	23	20	34.8	50.0	52.8	57.9	0.0	0.0	8.292469	119602	120869	1338-2281	0.746269	41.9
ndhD-trnL-UAG_p2	ndhD-trnL-UAG	2	GATCTGTATCTTTCTACCTGTAA	AGCAGCGTGTCTACCAATTT	23	20	34.8	45.0	52.8	57.8	0.0	0.0	8.353196	119602	120869	1331-2274	0.746269	41.9
ndhD-trnL-UAG_p3	ndhD-trnL-UAG	3	GATCTGTATCTTTCTACCTGTAA	CCTAAGAGCAGCGTGTCTAC	23	20	34.8	55.0	52.8	57.8	0.0	0.0	8.364487	119602	120869	1337-2280	0.746269	41.9
ndhD-trnL-UAG_p4	ndhD-trnL-UAG	4	GATCTGTATCTTTCTACCTGTAA	CTAAGAGCAGCGTGTCTACC	23	20	34.8	55.0	52.8	57.8	0.0	0.0	8.364487	119602	120869	1336-2279	0.746269	41.9
ndhD-trnL-UAG_p5	ndhD-trnL-UAG	5	GATCTGTATCTTTCTACCTGTAA	GAGCAGCGTGTCTACCAATT	23	20	34.8	50.0	52.8	58.3	0.0	0.0	8.441339	119602	120869	1332-2275	0.746269	41.9
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	TAGACACGCTGCTCTTAGGA	TGCCGCTAAAGAGCCTTTTT	20	20	50.0	45.0	57.9	58.4	0.0	5.6	0.508119	120950	121382	446-701	0.940299	85.5
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	TAGACACGCTGCTCTTAGGA	TTTACTGCCGCTAAAGAGCC	20	20	50.0	50.0	57.9	58.3	0.0	0.0	0.390115	120950	121382	451-706	0.880597	84.1
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	AAATTGGTAGACACGCTGCT	TTTACTGCCGCTAAAGAGCC	20	20	45.0	50.0	57.8	58.3	0.0	0.0	0.450841	120950	121382	458-713	0.880597	83.6
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	GGTAGACACGCTGCTCTTAG	TTTACTGCCGCTAAAGAGCC	20	20	55.0	50.0	57.8	58.3	0.0	0.0	0.462132	120950	121382	453-708	0.880597	83.5
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	GTAGACACGCTGCTCTTAGG	TTTACTGCCGCTAAAGAGCC	20	20	55.0	50.0	57.8	58.3	0.0	0.0	0.462132	120950	121382	452-707	0.880597	83.5
psaC-rps15_p1	psaC-rps15	1	AATACATCCGTGGGACAAGC	TTCTGGGAAAACGTCAACGA	20	20	50.0	45.0	58.0	57.7	0.0	9.9	0.351109	124497	125146	637-850	0.985075	88.6
psaC-rps15_p2	psaC-rps15	2	GGGACAAGCTCGTACACATT	TTCTGGGAAAACGTCAACGA	20	20	50.0	45.0	57.9	57.7	0.0	9.9	0.407485	124497	125146	626-839	0.985075	88.1
psaC-rps15_p3	psaC-rps15	3	ACAAGCTCGTACACATTGGG	TTCTGGGAAAACGTCAACGA	20	20	50.0	45.0	58.2	57.7	0.0	9.9	0.504312	124497	125146	623-836	0.985075	87.4
psaC-rps15_p4	psaC-rps15	4	CAAGCTCGTACACATTGGGT	TTCTGGGAAAACGTCAACGA	20	20	50.0	45.0	58.2	57.7	0.0	9.9	0.504312	124497	125146	622-835	0.985075	87.4
psaC-rps15_p5	psaC-rps15	5	ACATCCGTCCCAAGGTATCA	TTCTGGGAAAACGTCAACGA	20	20	50.0	45.0	58.1	57.7	0.0	9.9	0.438593	124497	125146	662-875	0.970149	87.3