primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ycf45_p1	ycf45	1	GGTTCATTTGGTGCAGGAGA	GGAGAGGTGGTAGAGTTGGT	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.150320	93193	94821	2468-2468	1.000000	83.5
ycf45_p2	ycf45	2	GGTTCATTTGGTGCAGGAGA	TGGAGAGGTGGTAGAGTTGG	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.151716	93193	94821	2469-2469	1.000000	83.5
ycf45_p3	ycf45	3	GGTTCATTTGGTGCAGGAGA	TTTCAGCATCTTCATCCGGG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.132986	93193	94821	2304-2304	0.500000	58.9
ycf45_p4	ycf45	4	TGAGTTCAAATGAGTGCGCT	TTTCAGCATCTTCATCCGGG	20	20	45.0	50.0	58.1	58.0	9.7	0.0	0.159774	93193	94821	2059-2059	0.500000	58.7
ycf45_p5	ycf45	5	ATGTGCCAACCATAGGTGAC	TTTCAGCATCTTCATCCGGG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.171457	93193	94821	2276-2276	0.500000	58.6
trnS(tga)-psaD_p1	trnS(tga)-psaD	1	CCGCAAATTACACGTGCATT	GATGGTCGTGAAGCAGCTAA	20	20	45.0	50.0	58.0	58.0	17.2	0.0	0.034308	95269	95720	1283-1283	0.500000	59.7
trnS(tga)-psaD_p2	trnS(tga)-psaD	2	CCCGGATGAAGATGCTGAAA	GATGGTCGTGAAGCAGCTAA	20	20	50.0	50.0	58.0	58.0	0.7	0.0	0.052831	95269	95720	732-732	0.500000	59.6
trnS(tga)-psaD_p3	trnS(tga)-psaD	3	ACCAACTCTACCACCTCTCC	GATGGTCGTGAAGCAGCTAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.070165	95269	95720	568-568	0.500000	59.4
trnS(tga)-psaD_p4	trnS(tga)-psaD	4	CCGCAAATTACACGTGCATT	TACGCAATGTTCCGATGTGT	20	20	45.0	45.0	58.0	57.9	17.2	0.0	0.111664	95269	95720	1881-1881	0.500000	59.1
trnS(tga)-psaD_p5	trnS(tga)-psaD	5	ATAGCAACACAGTTCCGCAA	GATGGTCGTGAAGCAGCTAA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.119269	95269	95720	1297-1297	0.500000	59.0
psaD-chlB_p1	psaD-chlB	1	CCAGTACTCCCACCAAATGT	ACCTGCGTACATCCAGTATG	20	20	50.0	50.0	57.1	57.1	0.0	0.0	1.769911	96147	96359	299-300	1.000000	73.8
psaD-chlB_p2	psaD-chlB	2	CCAGTACTCCCACCAAATGT	GGACCTGCGTACATCCAGTA	20	20	50.0	55.0	57.1	58.9	0.0	0.0	1.770326	96147	96359	301-302	1.000000	73.8
psaD-chlB_p3	psaD-chlB	3	CCCACCAAATGTAGGAGAAGG	ACCTGCGTACATCCAGTATG	21	20	52.4	50.0	58.0	57.1	0.0	0.0	1.895560	96147	96359	291-292	1.000000	72.8
psaD-chlB_p4	psaD-chlB	4	CCCACCAAATGTAGGAGAAGG	GGACCTGCGTACATCCAGTA	21	20	52.4	55.0	58.0	58.9	0.0	0.0	1.895976	96147	96359	293-294	1.000000	72.8
psaD-chlB_p5	psaD-chlB	5	CACCAAATGTAGGAGAAGGCA	ACCTGCGTACATCCAGTATG	21	20	47.6	50.0	57.9	57.1	0.0	0.0	1.962682	96147	96359	289-290	1.000000	72.3
chlB_p1	chlB	1	CTCAGGGATGCTAAAGCGTT	GTCCTATCGCTTGCTTCCAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.054014	98218	98337	1132-1132	0.500000	59.6
chlB_p2	chlB	2	CTCAGGGATGCTAAAGCGTT	ACTTTTTCTTGCGAACCCCT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.097204	98218	98337	937-937	0.500000	59.2
chlB_p3	chlB	3	CTCAGGGATGCTAAAGCGTT	CGGTCTCTTTCCAGCATTGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.128979	98218	98337	868-868	0.500000	59.0
chlB_p4	chlB	4	CTCAGGGATGCTAAAGCGTT	GCTGCCCTGTCTACAAAGTT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.132278	98218	98337	1054-1054	0.500000	58.9
chlB_p5	chlB	5	ATCTTAGCCTCAGGGATGCT	GTCCTATCGCTTGCTTCCAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.139513	98218	98337	1140-1140	0.500000	58.9
orf441_p1	orf441	1	CAGGGCGTTGCTCTTTATCT	GTCCTATCGCTTGCTTCCAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.054014	96999	98324	2140-2141	1.000000	85.7
orf441_p2	orf441	2	CAGGGCGTTGCTCTTTATCT	CGGTCTCTTTCCAGCATTGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.128979	96999	98324	1876-1877	1.000000	85.4
orf441_p3	orf441	3	TCGTGCGACTTGTTCATGAT	GTCCTATCGCTTGCTTCCAA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.103024	96999	98324	2246-2247	1.000000	85.1
orf441_p4	orf441	4	ACACATCGGAACATTGCGTA	GTCCTATCGCTTGCTTCCAA	20	20	45.0	50.0	57.9	58.0	5.4	0.0	0.111677	96999	98324	2326-2327	1.000000	84.9
orf441_p5	orf441	5	CAGGGCGTTGCTCTTTATCT	ACTTTTTCTTGCGAACCCCT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.097204	96999	98324	1945-1945	0.500000	59.2
atpB_p1	atpB	1	GTCTGGTGCAATCACTCGTA	TGTCTTTTTACATTCATTGCTCA	20	23	50.0	30.4	57.6	55.1	0.0	0.0	6.284342	115009	116433	1528-1530	1.000000	43.7
atpB_p2	atpB	2	GTCTGGTGCAATCACTCGTA	GTTGTCTTTTTACATTCATTGCTCA	20	25	50.0	32.0	57.6	57.1	0.0	0.0	6.292696	115009	116433	1530-1532	1.000000	43.7
atpB_p3	atpB	3	GTCTGGTGCAATCACTCGTA	TGAGTTGTCTTTTTACATTCATTGC	20	25	50.0	32.0	57.6	57.1	0.0	0.0	6.292696	115009	116433	1533-1535	1.000000	43.7
atpB_p4	atpB	4	TCTGTCTGGTGCAATCACTC	TGTCTTTTTACATTCATTGCTCA	20	23	50.0	30.4	57.5	55.1	0.0	0.0	6.383011	115009	116433	1531-1533	1.000000	43.7
atpB_p5	atpB	5	GTCTGGTGCAATCACTCGTA	CTGAGTTGTCTTTTTACATTCATTGC	20	26	50.0	34.6	57.6	58.0	0.0	0.0	6.364073	115009	116433	1534-1536	1.000000	43.6
ycf92_p1	ycf92	1	GCCTTTGGCTCTTCCTTCTT	TGCGTATACCAATTTCGCCA	20	20	50.0	45.0	58.1	58.0	0.7	0.0	0.109083	138538	139332	1174-1175	1.000000	86.8
ycf92_p2	ycf92	2	GCCTTTGGCTCTTCCTTCTT	TGTCTCCCCAATGTACCTGT	20	20	50.0	50.0	58.1	58.0	0.7	0.0	0.122357	138538	139332	1359-1360	1.000000	86.2
ycf92_p3	ycf92	3	TATCGAAGACGGCGTCTACT	TGCGTATACCAATTTCGCCA	20	20	50.0	45.0	58.1	58.0	39.1	0.0	0.080201	138538	139332	1423-1423	0.500000	59.4
ycf92_p4	ycf92	4	TATCGAAGACGGCGTCTACT	TGTCTCCCCAATGTACCTGT	20	20	50.0	50.0	58.1	58.0	39.1	0.0	0.093476	138538	139332	1608-1608	0.500000	59.3
ycf92_p5	ycf92	5	TATCGAAGACGGCGTCTACT	TCTAACGCTCTCCTGACGAT	20	20	50.0	50.0	58.1	58.0	39.1	0.0	0.094431	138538	139332	1629-1629	0.500000	59.2
cpcS_p1	cpcS	1	TCCGAGGCTAAGAAAACCCT	ATTATGCGCGCGGATTAGAT	20	20	50.0	45.0	58.3	58.0	0.0	40.3	0.357198	141227	141685	917-917	0.500000	57.1
cpcS_p2	cpcS	2	CGCAACTCTCTGGGTTGTAT	ATTATGCGCGCGGATTAGAT	20	20	50.0	45.0	57.6	58.0	11.3	40.3	0.396806	141227	141685	1176-1176	0.500000	56.8
cpcS_p3	cpcS	3	TCCGAGGCTAAGAAAACCCT	AGCTTGCCACAAGACCTAAC	20	20	50.0	50.0	58.3	58.1	0.0	11.4	0.449493	141227	141685	1214-1214	0.500000	56.4
cpcS_p4	cpcS	4	CGCAACTCTCTGGGTTGTAT	AGCTTGCCACAAGACCTAAC	20	20	50.0	50.0	57.6	58.1	11.3	11.4	0.489101	141227	141685	1473-1473	0.500000	56.1
cpcS_p5	cpcS	5	TTCCGAGGCTAAGAAAACCC	ATTATGCGCGCGGATTAGAT	20	20	50.0	45.0	57.5	58.0	0.0	40.3	0.494887	141227	141685	918-918	0.500000	56.0
orf768_p1	orf768	1	CTCCTTGCTGATGCTCAACT	AGGAAATTGCTCGAGACGTC	20	20	50.0	50.0	57.9	58.0	0.0	18.7	0.113232	141706	144012	2690-2690	1.000000	86.2
orf768_p2	orf768	2	TGAGTCATCCATTGGCCATG	AGGAAATTGCTCGAGACGTC	20	20	50.0	50.0	57.9	58.0	6.1	18.7	0.063840	141706	144012	2403-2403	0.500000	59.5
orf768_p3	orf768	3	TGAGTCATCCATTGGCCATG	ACTTCTGCATGTGGGTCAAG	20	20	50.0	50.0	57.9	58.1	6.1	0.0	0.159919	141706	144012	2571-2571	0.500000	58.7
orf768_p4	orf768	4	TGAGTCATCCATTGGCCATG	CTGCATGTGGGTCAAGTTCT	20	20	50.0	50.0	57.9	58.1	6.1	0.0	0.159919	141706	144012	2567-2567	0.500000	58.7
orf768_p5	orf768	5	TGAGTCATCCATTGGCCATG	CTTCTGCATGTGGGTCAAGT	20	20	50.0	50.0	57.9	58.1	6.1	0.0	0.159919	141706	144012	2570-2570	0.500000	58.7
orf402_p1	orf402	1	GCTGACGTATTACACCATCAGA	TGAAACTTTCATCTCATACGGCT	22	23	45.5	39.1	58.0	58.2	14.1	0.0	5.209492	145528	146730	1279-1282	1.000000	46.4
orf402_p2	orf402	2	AGCTGACGTATTACACCATCAG	TGAAACTTTCATCTCATACGGCT	22	23	45.5	39.1	58.0	58.2	0.0	0.0	5.214690	145528	146730	1280-1283	1.000000	46.4
orf402_p3	orf402	3	GCTGACGTATTACACCATCAGA	TGAAACTTTCATCTCATACGGC	22	22	45.5	40.9	58.0	56.8	14.1	0.0	5.248671	145528	146730	1279-1282	1.000000	46.4
orf402_p4	orf402	4	AGCTGACGTATTACACCATCAG	TGAAACTTTCATCTCATACGGC	22	22	45.5	40.9	58.0	56.8	0.0	0.0	5.253869	145528	146730	1280-1283	1.000000	46.4
orf402_p5	orf402	5	AGCTGACGTATTACACCATCA	TGAAACTTTCATCTCATACGGCT	21	23	42.9	39.1	56.8	58.2	0.0	0.0	5.331456	145528	146730	1280-1283	1.000000	46.4
cfxQ-trnC(gca)_p1	cfxQ-trnC(gca)	1	ACAGTCAGAAGATATTATGAAAAGCA	CCTTACCACTTGGCCATACC	26	20	30.8	55.0	57.0	57.9	0.0	6.1	7.077798	152849	152903	124-124	1.000000	44.5
cfxQ-trnC(gca)_p2	cfxQ-trnC(gca)	2	ACAGTCAGAAGATATTATGAAAAGCAG	CCTTACCACTTGGCCATACC	27	20	33.3	55.0	57.9	57.9	0.0	6.1	7.159959	152849	152903	124-124	1.000000	44.5
cfxQ-trnC(gca)_p3	cfxQ-trnC(gca)	3	ACAGTCAGAAGATATTATGAAAAGCA	TTGCAATCCTCTGCCTTACC	26	20	30.8	50.0	57.0	57.9	0.0	0.0	7.153786	152849	152903	137-137	1.000000	44.1
cfxQ-trnC(gca)_p4	cfxQ-trnC(gca)	4	ACAGTCAGAAGATATTATGAAAAGCA	TCGAACTGGGGGTAAAGGAT	26	20	30.8	50.0	57.0	58.0	0.0	0.0	7.063882	152849	152903	157-157	1.000000	43.6
cfxQ-trnC(gca)_p5	cfxQ-trnC(gca)	5	ACAGTCAGAAGATATTATGAAAAGCAG	TCGAACTGGGGGTAAAGGAT	27	20	33.3	50.0	57.9	58.0	0.0	0.0	7.146044	152849	152903	157-157	1.000000	43.6
trnC(gca)-trnL(taa)_p1	trnC(gca)-trnL(taa)	1	ATCCTTTACCCCCAGTTCGA	GTCTACCATTCCACCACACC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.148292	152975	153013	97-97	1.000000	83.8
trnC(gca)-trnL(taa)_p2	trnC(gca)-trnL(taa)	2	GGTATGGCCAAGTGGTAAGG	GTCTACCATTCCACCACACC	20	20	55.0	55.0	57.9	57.9	11.0	0.0	0.162207	152975	153013	130-130	1.000000	82.4
trnC(gca)-trnL(taa)_p3	trnC(gca)-trnL(taa)	3	GGTAAGGCAGAGGATTGCAA	GTCTACCATTCCACCACACC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.238195	152975	153013	117-117	1.000000	82.2
trnC(gca)-trnL(taa)_p4	trnC(gca)-trnL(taa)	4	TGCAAATCCTTTACCCCCAG	GTCTACCATTCCACCACACC	20	20	50.0	55.0	57.8	57.9	0.0	0.0	0.339554	152975	153013	102-102	1.000000	82.0
trnC(gca)-trnL(taa)_p5	trnC(gca)-trnL(taa)	5	AGGCAGAGGATTGCAAATCC	GTCTACCATTCCACCACACC	20	20	50.0	55.0	58.2	57.9	1.7	0.0	0.336588	152975	153013	113-113	1.000000	81.6
ycf80-ssrA_p1	ycf80-ssrA	1	AACTGCAACGACTCAAGGTT	GAGGGCATGTAACTCAGTGG	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.068225	156210	156974	1986-1990	1.000000	84.2
ycf80-ssrA_p2	ycf80-ssrA	2	AACTGCAACGACTCAAGGTT	CGTTTTCCCGTGGCTTAGTA	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.121553	156210	156974	1721-1725	1.000000	83.9
ycf80-ssrA_p3	ycf80-ssrA	3	AACTGCAACGACTCAAGGTT	TACGTTTTCCCGTGGCTTAG	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.121553	156210	156974	1723-1727	1.000000	83.9
ycf80-ssrA_p4	ycf80-ssrA	4	AGTCGAATCTGCTTGGCAAA	GAGGGCATGTAACTCAGTGG	20	20	45.0	55.0	58.1	58.0	0.0	0.0	0.133619	156210	156974	1508-1512	1.000000	83.9
ycf80-ssrA_p5	ycf80-ssrA	5	CCTGGAAATATGACCGTCCG	GAGGGCATGTAACTCAGTGG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.163973	156210	156974	1661-1661	0.500000	58.7
orf88-apcF_p1	orf88-apcF	1	CCACTGAGTTACATGCCCTC	AAGTGTCTCGCAATCCATCC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.059677	157815	158374	1258-1258	0.500000	59.5
orf88-apcF_p2	orf88-apcF	2	CCACTGAGTTACATGCCCTC	AAGAATTCCCACCGGGTCTA	20	20	55.0	50.0	58.0	58.0	0.0	6.3	0.068705	157815	158374	1129-1129	0.500000	59.5
orf88-apcF_p3	orf88-apcF	3	CCACTGAGTTACATGCCCTC	AGAATTCCCACCGGGTCTAA	20	20	55.0	50.0	58.0	58.0	0.0	6.3	0.068705	157815	158374	1128-1128	0.500000	59.5
orf88-apcF_p4	orf88-apcF	4	CCACTGAGTTACATGCCCTC	TAAGAATTCCCACCGGGTCT	20	20	55.0	50.0	58.0	58.0	0.0	6.3	0.068705	157815	158374	1130-1130	0.500000	59.5
orf88-apcF_p5	orf88-apcF	5	CTAAGCCACGGGAAAACGTA	AAGTGTCTCGCAATCCATCC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.113005	157815	158374	1521-1521	0.500000	59.1
ycf23_p1	ycf23	1	AGAACCTACGGTAAGCCCTT	CCACCAGCAGAGGTTGATAC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.081447	165076	165924	1645-1645	1.000000	85.3
ycf23_p2	ycf23	2	AGAACCTACGGTAAGCCCTT	CCAGCAGAGGTTGATACACC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.081447	165076	165924	1642-1642	1.000000	85.3
ycf23_p3	ycf23	3	ATCCTGTCAGCTTGCTTGAG	CCACCAGCAGAGGTTGATAC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.140740	165076	165924	1677-1677	0.500000	58.9
ycf23_p4	ycf23	4	ATCCTGTCAGCTTGCTTGAG	CCAGCAGAGGTTGATACACC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.140740	165076	165924	1674-1674	0.500000	58.9
ycf23_p5	ycf23	5	CCTGTCAGCTTGCTTGAGAA	CCAGCAGAGGTTGATACACC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.141624	165076	165924	1672-1672	0.500000	58.9
psaM-rrs_p1	psaM-rrs	1	TGCAACGAAAATTTGGCTGT	CGTCCGCCACTAAGGTAAAA	20	20	40.0	50.0	57.4	57.9	0.0	0.0	0.676513	187357	188235	990-994	1.000000	83.9
psaM-rrs_p2	psaM-rrs	2	TGCAACGAAAATTTGGCTGT	AACCTTCGTTCGACTTGCAT	20	20	40.0	45.0	57.4	58.1	0.0	0.0	0.714393	187357	188235	972-976	1.000000	83.6
psaM-rrs_p3	psaM-rrs	3	TGCAACGAAAATTTGGCTGT	CTTCGTTCGACTTGCATGTG	20	20	40.0	50.0	57.4	57.8	0.0	10.4	0.810699	187357	188235	969-973	1.000000	82.8
psaM-rrs_p4	psaM-rrs	4	TGCAACGAAAATTTGGCTGT	TGCATGTGTTAGGCATACCG	20	20	40.0	50.0	57.4	58.3	0.0	0.0	0.930829	187357	188235	957-961	1.000000	81.9
psaM-rrs_p5	psaM-rrs	5	TGCAACGAAAATTTGGCTGT	AAACCTTCGTTCGACTTGCA	20	20	40.0	45.0	57.4	58.3	0.0	0.0	0.934352	187357	188235	973-977	1.000000	81.8