primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6341	7666	1399-1428	1.000000	80.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	6341	7666	1410-1439	1.000000	79.8
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CCACAGCTGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.6	58.1	23.3	16.9	0.491095	6341	7666	1420-1449	1.000000	78.1
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCCACAGCTGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.4	58.1	23.3	16.9	0.499524	6341	7666	1421-1450	1.000000	78.1
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CCAAGAGCACCTTCATTCCT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.5	58.1	0.0	16.9	0.579996	6341	7666	1464-1493	1.000000	77.4
rps2-rpoC2_p1	rps2-rpoC2	1	TGTTCCAATATCTTCTTGTCA	ACGATACACCAGAACACTCG	21	20	33.3	50.0	52.5	57.4	0.0	0.0	7.161299	17164	17334	209-219	1.000000	46.4
rps2-rpoC2_p2	rps2-rpoC2	2	TGTTCCAATATCTTCTTGTCA	CGATACACCAGAACACTCGT	21	20	33.3	50.0	52.5	57.4	0.0	0.0	7.161299	17164	17334	208-218	1.000000	46.4
rps2-rpoC2_p3	rps2-rpoC2	3	GATGTTCCAATATCTTCTTGTCA	ACGATACACCAGAACACTCG	23	20	34.8	50.0	54.5	57.4	0.0	0.0	7.180768	17164	17334	211-221	1.000000	46.3
rps2-rpoC2_p4	rps2-rpoC2	4	GATGTTCCAATATCTTCTTGTCA	CGATACACCAGAACACTCGT	23	20	34.8	50.0	54.5	57.4	0.0	0.0	7.180768	17164	17334	210-220	1.000000	46.3
rps2-rpoC2_p5	rps2-rpoC2	5	TGTTCCAATATCTTCTTGTCA	TCCACGATACACCAGAACAC	21	20	33.3	50.0	52.5	57.3	0.0	0.0	7.266024	17164	17334	212-222	1.000000	46.3
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTCTTCATTCT	20	21	50.0	42.9	58.2	57.0	0.0	0.0	2.120636	29765	30913	1308-1311	1.000000	64.1
petN-psbM_p2	petN-psbM	2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTCTTCATTCT	20	21	50.0	42.9	57.6	57.0	0.0	0.0	2.320150	29765	30913	1247-1250	1.000000	62.5
petN-psbM_p3	petN-psbM	3	CCCTCGTAGTATGGGGAAGA	TGCTACTGCACTCTTCATTCT	20	21	55.0	42.9	57.6	57.0	10.1	0.0	2.345982	29765	30913	1257-1260	1.000000	62.3
petN-psbM_p4	petN-psbM	4	TCCCTCGTAGTATGGGGAAG	TGCTACTGCACTCTTCATTCT	20	21	55.0	42.9	57.6	57.0	10.1	0.0	2.345982	29765	30913	1258-1261	1.000000	62.3
petN-psbM_p5	petN-psbM	5	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTCTTCATTC	20	20	50.0	45.0	58.2	55.5	0.0	0.0	2.667062	29765	30913	1308-1311	1.000000	59.7
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGAATGAAGAGTGCAGTAGC	AGTTCAATCGGTCAGAGCAC	20	20	45.0	50.0	55.2	57.9	0.0	0.0	2.904445	31019	32179	1274-1296	1.000000	58.1
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AGAATGAAGAGTGCAGTAGC	TACACCTTATTTCCCCGGGA	20	20	45.0	50.0	55.2	57.7	0.0	26.3	3.085551	31019	32179	1298-1320	1.000000	56.6
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	ACTAGAATGAAGAGTGCAGTAGC	AGTTCAATCGGTCAGAGCAC	23	20	43.5	50.0	58.0	57.9	0.0	0.0	3.090976	31019	32179	1277-1299	1.000000	56.6
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AGAATGAAGAGTGCAGTAGC	GTACACCTTATTTCCCCGGG	20	20	45.0	55.0	55.2	57.7	0.0	15.8	3.155336	31019	32179	1299-1321	1.000000	56.0
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AGAATGAAGAGTGCAGTAGC	TCCCCGGGATTGTAGTTCAA	20	20	45.0	50.0	55.2	58.3	0.0	26.3	3.159369	31019	32179	1287-1309	1.000000	56.0
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	33809	35300	1572-1642	1.000000	81.9
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	GTGGTAGAGTAACGCCATGG	GACCGGACCAACCTACAAAA	20	20	55.0	50.0	58.1	57.7	7.6	9.5	0.323334	33809	35300	1586-1656	1.000000	81.8
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.332292	33809	35300	1575-1645	1.000000	81.8
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	TCAGTGGTAGAGTAACGCCA	GACCGGACCAACCTACAAAA	20	20	50.0	50.0	58.1	57.7	0.0	9.5	0.345488	33809	35300	1589-1659	1.000000	81.6
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GTGGTAGAGTAACGCCATGG	TAGACCGGACCAACCTACAA	20	20	55.0	50.0	58.1	57.7	7.6	9.5	0.371563	33809	35300	1588-1658	1.000000	81.4
psbC-trnS-UGA_p1	psbC-trnS-UGA	1	CATTTGTGGCATGCAGGAAG	GATGGCTCCGGTCTTGAAAA	20	20	50.0	50.0	58.0	58.2	21.4	0.0	0.183366	37732	37949	393-396	1.000000	80.4
psbC-trnS-UGA_p2	psbC-trnS-UGA	2	CATTTGTGGCATGCAGGAAG	GGGTTCGAATCCCTCTCTCT	20	20	50.0	55.0	58.0	58.2	21.4	17.2	0.221511	37732	37949	343-346	1.000000	80.3
psbC-trnS-UGA_p3	psbC-trnS-UGA	3	ATTTGTGGCATGCAGGAAGA	GATGGCTCCGGTCTTGAAAA	20	20	45.0	50.0	58.1	58.2	21.4	0.0	0.251296	37732	37949	392-395	1.000000	79.8
psbC-trnS-UGA_p4	psbC-trnS-UGA	4	ATTTGTGGCATGCAGGAAGA	GGGTTCGAATCCCTCTCTCT	20	20	45.0	55.0	58.1	58.2	21.4	17.2	0.289441	37732	37949	342-345	1.000000	79.8
psbC-trnS-UGA_p5	psbC-trnS-UGA	5	CCTGTTCTTTCCATGACCCC	GATGGCTCCGGTCTTGAAAA	20	20	55.0	50.0	58.2	58.2	0.0	0.0	0.339360	37732	37949	321-324	1.000000	79.5
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	49062	49774	763-793	1.000000	81.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	AAAGCCAGCTATCGGAATCG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.133544	49062	49774	808-838	1.000000	81.8
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	49062	49774	771-801	1.000000	81.3
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	AAAGCCAGCTATCGGAATCG	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.198352	49062	49774	816-846	1.000000	81.2
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	49062	49774	795-825	1.000000	80.9
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCAGGTAGA	AGTTGGATAGGATGGCCCTT	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.089643	50803	51526	866-877	1.000000	83.2
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	GGGATAGCTCAGCAGGTAGA	ATCATCCACGCTTGAAACGA	20	20	55.0	45.0	58.0	57.9	0.0	0.0	0.095812	50803	51526	901-912	1.000000	83.0
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.139034	50803	51526	830-841	1.000000	83.0
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	GGGATAGCTCAGCAGGTAGA	TCATCCACGCTTGAAACGAA	20	20	55.0	45.0	58.0	58.1	0.0	0.0	0.130117	50803	51526	900-911	1.000000	82.8
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCTTGAAACGA	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.145202	50803	51526	865-876	1.000000	82.8
petA-psbJ_p1	petA-psbJ	1	TTGGCATCTGTTATCCTGGC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.9	57.6	0.0	4.1	0.422110	66337	67301	1074-1078	1.000000	78.5
petA-psbJ_p2	petA-psbJ	2	ACGTGTCCAAGGTCTTTTGT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	57.6	57.6	0.0	4.1	0.778282	66337	67301	1099-1103	1.000000	75.6
petA-psbJ_p3	petA-psbJ	3	CATCTGTTATCCTGGCCCAA	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.3	57.6	0.0	4.1	1.099728	66337	67301	1070-1074	1.000000	73.1
petA-psbJ_p4	petA-psbJ	4	TCCATTACGTGTCCAAGGTC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.2	57.6	0.0	4.1	1.119919	66337	67301	1105-1109	1.000000	72.8
petA-psbJ_p5	petA-psbJ	5	CCATTACGTGTCCAAGGTCT	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.2	57.6	0.0	4.1	1.127508	66337	67301	1104-1108	1.000000	72.8
psbB-psbT_p1	psbB-psbT	1	GCTGGTATTGACCCGGATTT	GAACTTTAGGCGGTTCTCGA	20	20	50.0	50.0	57.9	57.6	0.7	0.0	0.411721	77700	77897	372-376	1.000000	78.6
psbB-psbT_p2	psbB-psbT	2	GCTGGTATTGACCCGGATTT	TAGTCGGAACTTTAGGCGGT	20	20	50.0	50.0	57.9	58.5	0.7	0.0	0.503579	77700	77897	378-382	1.000000	77.9
psbB-psbT_p3	psbB-psbT	3	CCGGATTTGGATGCTCAAGT	GAACTTTAGGCGGTTCTCGA	20	20	50.0	50.0	58.2	57.6	0.0	0.0	0.603760	77700	77897	360-364	1.000000	77.2
psbB-psbT_p4	psbB-psbT	4	CCGGATTTGGATGCTCAAGT	TAGTCGGAACTTTAGGCGGT	20	20	50.0	50.0	58.2	58.5	0.0	0.0	0.695617	77700	77897	366-370	1.000000	76.4
psbB-psbT_p5	psbB-psbT	5	GCTGGTATTGACCCGGATTT	GGAACTTTAGGCGGTTCTCG	20	20	50.0	55.0	57.9	58.6	0.7	12.5	0.695844	77700	77897	373-377	1.000000	76.4
rpl14-rpl16_p1	rpl14-rpl16	1	ATCGGCGATTACTAGCTCCT	CTATTTCCATAGCGGCGTCA	20	20	50.0	50.0	58.1	57.9	18.1	9.4	0.236499	84815	84941	280-280	1.000000	79.3
rpl14-rpl16_p2	rpl14-rpl16	2	ATCGGCGATTACTAGCTCCT	CCATAGCGGCGTCAAAAATG	20	20	50.0	50.0	58.1	58.2	18.1	9.4	0.261256	84815	84941	274-274	1.000000	79.1
rpl14-rpl16_p3	rpl14-rpl16	3	ATCGGCGATTACTAGCTCCT	ATTTCCATAGCGGCGTCAAA	20	20	50.0	45.0	58.1	58.3	18.1	9.4	0.342351	84815	84941	278-278	1.000000	78.4
rpl14-rpl16_p4	rpl14-rpl16	4	ATCGGCGATTACTAGCTCCT	TCCATAGCGGCGTCAAAAAT	20	20	50.0	45.0	58.1	58.3	18.1	9.4	0.342351	84815	84941	275-275	1.000000	78.4
rpl14-rpl16_p5	rpl14-rpl16	5	ATCGGCGATTACTAGCTCCT	TTCCATAGCGGCGTCAAAAA	20	20	50.0	45.0	58.1	58.5	18.1	9.4	0.562735	84815	84941	276-276	1.000000	76.7
rpl2-rpl23_p1	rpl2-rpl23	1	CACTTGACTGTCTACGGCTC	CGCTTCAACCGGGTTATTCT	20	20	55.0	50.0	58.0	58.3	9.5	13.0	0.281865	89556	89579	134-134	1.000000	78.3
rpl2-rpl23_p2	rpl2-rpl23	2	GACTGTCTACGGCTCCATTG	CGCTTCAACCGGGTTATTCT	20	20	55.0	50.0	58.1	58.3	0.0	13.0	0.337942	89556	89579	129-129	1.000000	77.9
rpl2-rpl23_p3	rpl2-rpl23	3	CACTTGACTGTCTACGGCTC	ATCATTACGCTTCAACCGGG	20	20	55.0	50.0	58.0	58.3	9.5	6.3	0.352333	89556	89579	141-141	1.000000	77.7
rpl2-rpl23_p4	rpl2-rpl23	4	GACTGTCTACGGCTCCATTG	ATCATTACGCTTCAACCGGG	20	20	55.0	50.0	58.1	58.3	0.0	6.3	0.408411	89556	89579	136-136	1.000000	77.3
rpl2-rpl23_p5	rpl2-rpl23	5	TGCTCGGGGTAGAAGTTTTG	CGCTTCAACCGGGTTATTCT	20	20	50.0	50.0	57.8	58.3	0.0	13.0	0.444749	89556	89579	105-105	1.000000	77.2
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	TCAAAGCGAGCCCTTTCATT	TCGTATCCCCATGAATCCGA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.094237	112323	114404	2136-2825	1.000000	84.1
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	GGGAACGGGTTACCTACTCT	TCGTATCCCCATGAATCCGA	20	20	55.0	50.0	58.1	58.0	5.5	0.0	0.145972	112323	114404	2162-2851	1.000000	83.7
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	ACGACCAGTCAGTTAACAGC	TCGTATCCCCATGAATCCGA	20	20	50.0	50.0	57.8	58.0	8.6	0.0	0.154334	112323	114404	2063-2752	1.000000	83.6
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	AGGATTCGAACCTACGACCA	TCGTATCCCCATGAATCCGA	20	20	50.0	50.0	58.2	58.0	15.0	0.0	0.160569	112323	114404	2076-2765	1.000000	83.6
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	TCAAAGCGAGCCCTTTCATT	CGTATCCCCATGAATCCGAC	20	20	45.0	55.0	58.1	57.9	0.0	0.0	0.170586	112323	114404	2135-2824	1.000000	83.5
ndhF-rpl32_p1	ndhF-rpl32	1	GCTAAGGGTATGATCCATGCA	AAAAAGCTTTTACCGCTGCC	21	20	47.6	45.0	57.6	57.9	0.0	24.1	1.572455	116631	117767	1239-1275	1.000000	71.8
ndhF-rpl32_p2	ndhF-rpl32	2	GCTAAGGGTATGATCCATGCA	GCCCAAGACCCCTTCTTTTT	21	20	47.6	50.0	57.6	58.3	0.0	0.0	1.706508	116631	117767	1222-1258	1.000000	70.8
ndhF-rpl32_p3	ndhF-rpl32	3	AGCTAAGGGTATGATCCATGC	AAAAAGCTTTTACCGCTGCC	21	20	47.6	45.0	57.3	57.9	0.0	24.1	1.859124	116631	117767	1240-1276	1.000000	69.5
ndhF-rpl32_p4	ndhF-rpl32	4	AGCTAAGGGTATGATCCATGC	GCCCAAGACCCCTTCTTTTT	21	20	47.6	50.0	57.3	58.3	0.0	0.0	1.993177	116631	117767	1223-1259	1.000000	68.5
ndhF-rpl32_p5	ndhF-rpl32	5	GCTAAGGGTATGATCCATGCAT	AAAAAGCTTTTACCGCTGCC	22	20	45.5	45.0	58.0	57.9	0.0	24.1	2.188345	116631	117767	1239-1275	1.000000	66.9
ndhD-psaC_p1	ndhD-psaC	1	CCGCAGATATTGGCAAGACT	ACCGATTTCTTGAGTGTCCG	20	20	50.0	50.0	58.0	57.9	6.0	0.0	0.121244	121766	121876	250-251	1.000000	81.3
ndhD-psaC_p2	ndhD-psaC	2	CCGCAGATATTGGCAAGACT	AAGAGATGTGAATCCGCCTG	20	20	50.0	50.0	58.0	57.7	6.0	0.0	0.363314	121766	121876	274-275	1.000000	79.1
ndhD-psaC_p3	ndhD-psaC	3	AAGAACCCGCAGATATTGGC	ACCGATTTCTTGAGTGTCCG	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.399948	121766	121876	256-257	1.000000	79.0
ndhD-psaC_p4	ndhD-psaC	4	CCGCAGATATTGGCAAGACT	GAGATGTGAATCCGCCTGTC	20	20	50.0	55.0	58.0	58.4	6.0	0.0	0.464603	121766	121876	272-273	1.000000	78.3
ndhD-psaC_p5	ndhD-psaC	5	CCGCAGATATTGGCAAGACT	GCCTGTCCAACCGATTTCTT	20	20	50.0	50.0	58.0	58.5	6.0	0.0	0.506230	121766	121876	259-260	1.000000	78.1
ndhA_p1	ndhA	1	TGACCATAATTCATGAACCCAGT	GCCCACCGGGTTTTATCAAT	23	20	39.1	50.0	57.8	58.5	11.1	6.3	3.710938	124446	126597	2390-2392	1.000000	51.1
ndhA_p2	ndhA	2	ACCCAGTTATCATAGGGAACA	GCCCACCGGGTTTTATCAAT	21	20	42.9	50.0	55.7	58.5	2.9	6.3	3.780350	124446	126597	2374-2376	1.000000	50.6
ndhA_p3	ndhA	3	GAACCCAGTTATCATAGGGAACA	GCCCACCGGGTTTTATCAAT	23	20	43.5	50.0	57.6	58.5	2.9	6.3	3.887155	124446	126597	2376-2378	1.000000	49.7
ndhA_p4	ndhA	4	TGAACCCAGTTATCATAGGGAAC	GCCCACCGGGTTTTATCAAT	23	20	43.5	50.0	57.6	58.5	2.9	6.3	3.887155	124446	126597	2377-2379	1.000000	49.7
ndhA_p5	ndhA	5	AACCCAGTTATCATAGGGAACA	GCCCACCGGGTTTTATCAAT	22	20	40.9	50.0	56.5	58.5	2.9	6.3	4.010458	124446	126597	2375-2377	1.000000	48.8
ycf1_p1	ycf1	1	TCACAACTTCTTTCGTTCGGA	TCATGAATCGTCCATTCGAA	21	20	42.9	40.0	57.8	54.9	0.0	0.0	4.277548	130956	131075	186-186	1.000000	48.4
ycf1_p2	ycf1	2	TCACAACTTCTTTCGTTCGGA	TTCATGAATCGTCCATTCGA	21	20	42.9	40.0	57.8	54.9	0.0	3.4	4.277548	130956	131075	187-187	1.000000	48.3
ycf1_p3	ycf1	3	CACAACTTCTTTCGTTCGGAC	TCATGAATCGTCCATTCGAA	21	20	47.6	40.0	57.8	54.9	0.0	0.0	4.344223	130956	131075	185-185	1.000000	47.8
ycf1_p4	ycf1	4	CACAACTTCTTTCGTTCGGAC	TTCATGAATCGTCCATTCGA	21	20	47.6	40.0	57.8	54.9	0.0	3.4	4.344223	130956	131075	186-186	1.000000	47.8
ycf1_p5	ycf1	5	TCACAACTTCTTTCGTTCGGA	TTCATGAATCGTCCATTCGAA	21	21	42.9	38.1	57.8	55.7	0.0	3.4	4.461762	130956	131075	187-187	1.000000	46.9