primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5837	7239	1450-1476	1.000000	80.6
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5837	7239	1461-1487	1.000000	80.0
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	ATAAATCGAAGTGGGGCGTG	20	20	45.0	50.0	58.1	58.3	8.1	0.0	0.475629	5837	7239	1509-1535	1.000000	78.4
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CCACAGCTGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.6	58.1	23.3	16.9	0.491095	5837	7239	1471-1497	1.000000	78.3
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TCCACAGCTGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.4	58.1	23.3	16.9	0.499524	5837	7239	1472-1498	1.000000	78.3
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	CAATCCGACGCTTTAGTCCA	TGTTCGGAATACAAAGAAAACCG	20	23	50.0	39.1	58.0	57.9	0.0	0.0	3.102801	8562	9196	759-761	1.000000	56.3
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	AATCCGACGCTTTAGTCCAC	TGTTCGGAATACAAAGAAAACCG	20	23	50.0	39.1	58.0	57.9	0.0	0.0	3.104189	8562	9196	758-760	1.000000	56.3
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	GCTCGTACAACGGATTAGCA	TGTTCGGAATACAAAGAAAACCG	20	23	50.0	39.1	58.1	57.9	0.0	0.0	3.180946	8562	9196	777-779	1.000000	55.7
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	CAATCCGACGCTTTAGTCCA	CGAAGGACCCCTTAACTATTAGG	20	23	50.0	47.8	58.0	57.8	0.0	0.0	3.215757	8562	9196	738-740	1.000000	55.5
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	AATCCGACGCTTTAGTCCAC	CGAAGGACCCCTTAACTATTAGG	20	23	50.0	47.8	58.0	57.8	0.0	0.0	3.217145	8562	9196	737-739	1.000000	55.5
trnG-GCC_p1	trnG-GCC	1	GTCACCTGACAAAAGGCAGA	AGGAACAGTTGGAACGCTTT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.085464	9197	9964	1253-1281	1.000000	82.0
trnG-GCC_p2	trnG-GCC	2	CAATCCGACGCTTTAGTCCA	AGGAACAGTTGGAACGCTTT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.057365	9197	9964	1816-1842	1.000000	81.9
trnG-GCC_p3	trnG-GCC	3	AATCCGACGCTTTAGTCCAC	AGGAACAGTTGGAACGCTTT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.058753	9197	9964	1815-1841	1.000000	81.8
trnG-GCC_p4	trnG-GCC	4	CAATCCGACGCTTTAGTCCA	CAATGGACGCGTTTCATTCC	20	20	50.0	50.0	58.0	58.1	0.0	27.3	0.109531	9197	9964	1682-1682	0.500000	59.1
trnG-GCC_p5	trnG-GCC	5	AATCCGACGCTTTAGTCCAC	CAATGGACGCGTTTCATTCC	20	20	50.0	50.0	58.0	58.1	0.0	27.3	0.110919	9197	9964	1681-1681	0.500000	59.1
trnG-GCC-trnR-UCU_p1	trnG-GCC-trnR-UCU	1	TCCTGAGTTGACGTCGACTA	AGGTTTAGAAGACCTATGTCCT	20	22	50.0	40.9	57.8	55.6	33.2	0.0	4.548780	9965	10198	324-348	1.000000	50.0
trnG-GCC-trnR-UCU_p2	trnG-GCC-trnR-UCU	2	TAGCCTTCCAAGCTACCGAT	AGGTTTAGAAGACCTATGTCCT	20	22	50.0	40.9	58.2	55.6	0.0	0.0	4.584894	9965	10198	297-321	1.000000	49.9
trnG-GCC-trnR-UCU_p3	trnG-GCC-trnR-UCU	3	TCCTGAGTTGACGTCGACTA	AGAAGACCTATGTCCTATCCA	20	21	50.0	42.9	57.8	54.6	33.2	0.0	4.578862	9965	10198	318-342	1.000000	49.8
trnG-GCC-trnR-UCU_p4	trnG-GCC-trnR-UCU	4	TAGCCTTCCAAGCTACCGAT	AGAAGACCTATGTCCTATCCA	20	21	50.0	42.9	58.2	54.6	0.0	0.0	4.614976	9965	10198	291-315	1.000000	49.7
trnG-GCC-trnR-UCU_p5	trnG-GCC-trnR-UCU	5	TCCTGAGTTGACGTCGACTA	ACCAAAGGTTTAGAAGACCTATGT	20	24	50.0	37.5	57.8	57.6	33.2	0.0	4.624193	9965	10198	329-353	1.000000	49.4
rps2-rpoC2_p1	rps2-rpoC2	1	ACCAAAATGAACTCCCGCTT	ACATCAGAACAATCATTTCTGGG	20	23	45.0	39.1	58.0	57.1	0.0	11.2	3.914244	15424	15733	412-423	1.000000	51.4
rps2-rpoC2_p2	rps2-rpoC2	2	AATGAACTCCCGCTTCCATC	ACATCAGAACAATCATTTCTGGG	20	23	50.0	39.1	58.0	57.1	0.0	11.2	3.953743	15424	15733	407-418	1.000000	51.1
rps2-rpoC2_p3	rps2-rpoC2	3	CCCGCTTCCATCATCTCTTC	ACATCAGAACAATCATTTCTGGG	20	23	55.0	39.1	58.1	57.1	0.0	11.2	4.033299	15424	15733	399-410	1.000000	50.5
rps2-rpoC2_p4	rps2-rpoC2	4	CCGCTTCCATCATCTCTTCC	ACATCAGAACAATCATTTCTGGG	20	23	55.0	39.1	58.1	57.1	0.0	11.2	4.033299	15424	15733	398-409	1.000000	50.5
rps2-rpoC2_p5	rps2-rpoC2	5	ATATAAGGTGCCATTCGCGG	ACATCAGAACAATCATTTCTGGG	20	23	50.0	39.1	58.2	57.1	6.1	11.2	4.101210	15424	15733	453-464	1.000000	49.6
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.273256	27319	28185	899-932	1.000000	81.3
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	TTAAACCAGCCCAAGCAAGA	20	20	50.0	45.0	58.1	57.6	0.0	0.0	0.455463	27319	28185	905-938	1.000000	79.8
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTCCCCAGTTCAAATCCGG	TAAACCAGCCCAAGCAAGAC	20	20	50.0	50.0	58.1	58.4	0.0	0.0	0.465969	27319	28185	904-937	1.000000	79.7
trnC-GCA-petN_p4	trnC-GCA-petN	4	GGGGGACTGCAAATCCTTTT	CAGCCCAAGCAAGACTTACT	20	20	50.0	50.0	58.4	57.8	14.7	0.0	0.546619	27319	28185	917-950	1.000000	79.1
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTTCCCCAGTTCAAATCCGG	TTAGAGTCCACTTCGTCCCC	20	20	50.0	55.0	58.1	58.4	0.0	0.0	0.528422	27319	28185	961-994	1.000000	79.0
psbZ-trnG-UCC_p1	psbZ-trnG-UCC	1	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.8	57.8	0.0	1.1	0.374855	34583	35309	884-932	1.000000	79.2
psbZ-trnG-UCC_p2	psbZ-trnG-UCC	2	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	20	20	55.0	50.0	58.3	57.8	0.0	1.1	0.425837	34583	35309	882-930	1.000000	78.8
psbZ-trnG-UCC_p3	psbZ-trnG-UCC	3	AGTCTTTTTGGTGGGGATCC	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.4	57.8	1.9	1.1	0.774744	34583	35309	814-862	1.000000	76.2
psbZ-trnG-UCC_p4	psbZ-trnG-UCC	4	GTCTTTTTGGTGGGGATCCT	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.4	57.8	12.6	1.1	0.774744	34583	35309	813-861	1.000000	76.2
psbZ-trnG-UCC_p5	psbZ-trnG-UCC	5	TCTCCTGATGGTTGGTCAAC	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.1	57.8	5.3	1.1	1.028998	34583	35309	879-927	1.000000	74.0
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	CAGAGGCTTGGTTCGATCAA	20	20	50.0	50.0	57.9	57.9	4.1	0.0	0.214484	40657	41331	838-868	1.000000	81.4
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	20	20	50.0	45.0	57.9	57.8	4.1	0.0	0.279161	40657	41331	752-782	1.000000	81.2
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	ATAGCACTTACTCCCGGGAA	20	20	50.0	50.0	57.9	57.8	4.1	26.7	0.285355	40657	41331	794-824	1.000000	81.0
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	TAGCACTTACTCCCGGGAAT	20	20	50.0	50.0	57.9	57.8	4.1	26.7	0.285355	40657	41331	793-823	1.000000	81.0
psaA-ycf3_p5	psaA-ycf3	5	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	20	20	50.0	50.0	57.9	58.2	4.1	0.0	0.306932	40657	41331	756-786	1.000000	81.0
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	ATCCCCGCTTGAAACGTATC	20	20	55.0	50.0	58.0	58.1	10.3	0.0	0.053040	46218	46865	822-823	1.000000	80.9
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	ATCCCCGCTTGAAACGTATC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.102430	46218	46865	786-787	1.000000	80.6
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CTGGTAGAGCAGAGGACTGA	ATCCCCGCTTGAAACGTATC	20	20	55.0	50.0	57.9	58.1	2.5	0.0	0.186833	46218	46865	810-811	1.000000	79.9
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	GATACGGAAAGGATGGTCGG	ATCCCCGCTTGAAACGTATC	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.200932	46218	46865	840-841	1.000000	79.7
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	GAAAGGATGGTCGGGATAGC	ATCCCCGCTTGAAACGTATC	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.221875	46218	46865	834-835	1.000000	79.6
accD-psaI_p1	accD-psaI	1	CTCCACGCTTTCTTTCCCTT	AAAGAAGCCATTGCCATTGC	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.205977	56655	57214	671-676	1.000000	79.7
accD-psaI_p2	accD-psaI	2	CTCCACGCTTTCTTTCCCTT	ATTGCCATTGCCGGAAAAAC	20	20	50.0	45.0	58.1	58.2	0.0	5.0	0.295301	56655	57214	662-667	1.000000	79.0
accD-psaI_p3	accD-psaI	3	CTCCACGCTTTCTTTCCCTT	CATTGCCATTGCCGGAAAAA	20	20	50.0	45.0	58.1	58.2	0.0	5.0	0.296571	56655	57214	663-668	1.000000	78.9
accD-psaI_p4	accD-psaI	4	CTCCACGCTTTCTTTCCCTT	GCCATTGCCGGAAAAACTAG	20	20	50.0	50.0	58.1	57.7	0.0	5.0	0.380466	56655	57214	659-664	1.000000	78.3
accD-psaI_p5	accD-psaI	5	CTCCACGCTTTCTTTCCCTT	TGCCATTGCCGGAAAAACTA	20	20	50.0	45.0	58.1	58.4	0.0	5.0	0.480448	56655	57214	660-665	1.000000	77.5
psbF-psbE_p1	psbF-psbE	1	GTCCGTGAATAGCCAACCAT	TGTGTTTGGAAGTCCTCGAC	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.272687	61525	61533	185-185	1.000000	78.3
psbF-psbE_p2	psbF-psbE	2	ACGGTAGGTACAGCTAGTCC	TGTGTTTGGAAGTCCTCGAC	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.284188	61525	61533	201-201	1.000000	78.1
psbF-psbE_p3	psbF-psbE	3	GGTAGGTACAGCTAGTCCGT	TGTGTTTGGAAGTCCTCGAC	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.284188	61525	61533	199-199	1.000000	78.1
psbF-psbE_p4	psbF-psbE	4	GTCCGTGAATAGCCAACCAT	GTTTGGAAGTCCTCGACCAA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.288230	61525	61533	182-182	1.000000	78.1
psbF-psbE_p5	psbF-psbE	5	GTCCGTGAATAGCCAACCAT	TTGGAAGTCCTCGACCAAAC	20	20	50.0	50.0	58.0	57.8	0.0	0.7	0.288230	61525	61533	180-180	1.000000	78.1
rps19-rpl2_p1	rps19-rpl2	1	GCCCGGGACCAAGTTACTAT	GGGCCCCAATTGGTAGAAAA	20	20	55.0	50.0	59.2	58.1	29.3	22.9	1.219144	81152	81205	283-283	1.000000	70.8
rps19-rpl2_p2	rps19-rpl2	2	GCCCGGGACCAAGTTACTAT	TTGATTCTTCGTCGCCGTAG	20	20	55.0	50.0	59.2	58.1	29.3	0.0	1.260580	81152	81205	191-191	1.000000	70.8
rps19-rpl2_p3	rps19-rpl2	3	GCCCGGGACCAAGTTACTAT	AGAAAAAGACCCACAACCCC	20	20	55.0	50.0	59.2	57.9	29.3	0.0	1.246864	81152	81205	269-269	1.000000	70.6
rps19-rpl2_p4	rps19-rpl2	4	GCCCGGGACCAAGTTACTAT	GAAAAAGACCCACAACCCCT	20	20	55.0	50.0	59.2	57.9	29.3	0.0	1.246864	81152	81205	268-268	1.000000	70.6
rps19-rpl2_p5	rps19-rpl2	5	GCCCGGGACCAAGTTACTAT	CACAACCCCTTGGGGTTATC	20	20	55.0	55.0	59.2	58.2	29.3	35.0	1.317396	81152	81205	258-258	1.000000	70.1
ndhF_p1	ndhF	1	GGATGGGAATCAAGAAACTTCG	AGGATCTGGTGAATTAGAAACCA	22	23	45.5	39.1	57.4	57.2	0.0	0.0	6.401942	106782	108998	2296-2656	1.000000	40.5
ndhF_p2	ndhF	2	TCTGGATGGGAATCAAGAAACT	AGGATCTGGTGAATTAGAAACCA	22	23	40.9	39.1	57.1	57.2	0.0	0.0	6.714038	106782	108998	2299-2659	1.000000	40.5
ndhF_p3	ndhF	3	GGATGGGAATCAAGAAACTTCG	GGATCTGGTGAATTAGAAACCA	22	22	45.5	40.9	57.4	55.8	0.0	0.0	6.836656	106782	108998	2295-2655	1.000000	40.5
ndhF_p4	ndhF	4	GGATGGGAATCAAGAAACTTCG	AGGATCTGGTGAATTAGAAACCAT	22	24	45.5	37.5	57.4	57.7	0.0	0.0	6.955677	106782	108998	2296-2656	1.000000	40.5
ndhF_p5	ndhF	5	TCTGGATGGGAATCAAGAAACTT	AGGATCTGGTGAATTAGAAACCA	23	23	39.1	39.1	57.7	57.2	0.0	0.0	7.040281	106782	108998	2299-2659	1.000000	40.5
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	CTTGAGCATCTCGGTTCGAA	TCATCGACAACTAAGCTTATCA	20	22	50.0	36.4	58.0	55.0	0.0	6.6	4.960572	110358	110430	204-205	1.000000	41.5
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	CTTGAGCATCTCGGTTCGAA	TTTCATCGACAACTAAGCTT	20	20	50.0	35.0	58.0	52.9	0.0	3.5	5.141136	110358	110430	206-207	1.000000	41.2
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	TAGACACGCTGCTCTTAGGA	TCATCGACAACTAAGCTTATCA	20	22	50.0	36.4	57.9	55.0	0.0	6.6	5.081141	110358	110430	231-232	1.000000	41.0
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	AAATTGGTAGACACGCTGCT	TCATCGACAACTAAGCTTATCA	20	22	45.0	36.4	57.8	55.0	0.0	6.6	5.141868	110358	110430	238-239	1.000000	41.0
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	GTAGACACGCTGCTCTTAGG	TCATCGACAACTAAGCTTATCA	20	22	55.0	36.4	57.8	55.0	0.0	6.6	5.153159	110358	110430	232-233	1.000000	41.0
ccsA_p1	ccsA	1	CTTGAGCATCTCGGTTCGAA	TGCATTACCGGGAATGAGTG	20	20	50.0	50.0	58.0	58.0	0.0	38.4	0.040666	110431	111390	1662-1664	1.000000	80.7
ccsA_p2	ccsA	2	CTTGAGCATCTCGGTTCGAA	GCGGGACCACGAGAATTATT	20	20	50.0	50.0	58.0	58.1	0.0	6.6	0.054243	110431	111390	1451-1453	1.000000	80.7
ccsA_p3	ccsA	3	TGGCGGCATACTCTCTTCTA	TGCATTACCGGGAATGAGTG	20	20	50.0	50.0	57.9	58.0	8.0	38.4	0.104323	110431	111390	1636-1638	1.000000	80.2
ccsA_p4	ccsA	4	CTTGAGCATCTCGGTTCGAA	GCTCTTGACGACTGCAACTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.069701	110431	111390	1148-1149	1.000000	80.0
ccsA_p5	ccsA	5	CTTGAGCATCTCGGTTCGAA	CTTCACTTGCATTACCGGGA	20	20	50.0	50.0	58.0	57.9	0.0	6.3	0.111658	110431	111390	1671-1671	0.500000	59.1
ccsA-ndhD_p1	ccsA-ndhD	1	GCAATTGTAGCTTCTATGGGC	TGGTATTTACCCGGATTTCGT	21	21	47.6	42.9	57.4	57.1	7.4	0.7	3.543424	111391	111641	440-441	1.000000	53.8
ccsA-ndhD_p2	ccsA-ndhD	2	CCGCAATTGTAGCTTCTATGG	TGGTATTTACCCGGATTTCGT	21	21	47.6	42.9	57.1	57.1	7.4	0.7	3.796052	111391	111641	442-443	1.000000	51.8
ccsA-ndhD_p3	ccsA-ndhD	3	CGCAATTGTAGCTTCTATGGG	TGGTATTTACCCGGATTTCGT	21	21	47.6	42.9	57.1	57.1	7.4	0.7	3.796052	111391	111641	441-442	1.000000	51.8
ccsA-ndhD_p4	ccsA-ndhD	4	GCAATTGTAGCTTCTATGGGC	TGGTATTTACCCGGATTTCGTT	21	22	47.6	40.9	57.4	57.8	7.4	0.7	3.852119	111391	111641	440-441	1.000000	51.4
ccsA-ndhD_p5	ccsA-ndhD	5	GCAATTGTAGCTTCTATGGGC	TTGGTATTTACCCGGATTTCGT	21	22	47.6	40.9	57.4	57.8	7.4	0.7	3.852119	111391	111641	441-442	1.000000	51.4
ycf1_p1	ycf1	1	AGACCACTTTTGTTAATCCAGT	CCGTTCTATCTGAAACCTTGGT	22	22	36.4	45.5	55.6	58.1	0.0	0.0	6.506765	122039	122158	263-266	1.000000	42.1
ycf1_p2	ycf1	2	AGACCACTTTTGTTAATCCAGT	TCCGTTCTATCTGAAACCTTGG	22	22	36.4	45.5	55.6	57.8	0.0	0.0	6.648076	122039	122158	264-267	1.000000	42.1
ycf1_p3	ycf1	3	AGACCACTTTTGTTAATCCAGT	AGTGGTGTTCAACTATCCGA	22	20	36.4	45.0	55.6	56.2	0.0	0.0	6.252304	122039	122158	338-341	1.000000	41.6
ycf1_p4	ycf1	4	AGACCACTTTTGTTAATCCAGT	AGTGGTGTTCAACTATCCGAA	22	21	36.4	42.9	55.6	57.0	0.0	0.0	6.487414	122039	122158	338-341	1.000000	41.6
ycf1_p5	ycf1	5	AGACCACTTTTGTTAATCCAGTT	AGTGGTGTTCAACTATCCGA	23	20	34.8	45.0	56.3	56.2	0.0	0.0	6.517310	122039	122158	338-341	1.000000	41.6