primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK_p1	matK	1	TCAGAATGGAAAACGTGAAAGT	CAACACCCTGTTCTGACCAT	22	20	36.4	50.0	56.5	57.7	0.0	0.0	3.787067	1683	3218	1699-1699	1.000000	50.0
matK_p2	matK	2	TCAGAATGGAAAACGTGAAAGT	AATGCAACACCCTGTTCTGA	22	20	36.4	45.0	56.5	57.6	0.0	0.0	3.873641	1683	3218	1703-1703	1.000000	49.3
matK_p3	matK	3	TCAGAATGGAAAACGTGAAAGT	ATGCAACACCCTGTTCTGAC	22	20	36.4	50.0	56.5	58.4	0.0	0.0	3.901127	1683	3218	1702-1702	1.000000	49.1
matK_p4	matK	4	ACATTTCAGAATGGAAAACGTGA	CAACACCCTGTTCTGACCAT	23	20	34.8	50.0	57.2	57.7	0.0	0.0	4.042883	1683	3218	1704-1704	1.000000	47.9
matK_p5	matK	5	AGGGAACATTTCAGAATGGA	CAACACCCTGTTCTGACCAT	20	20	40.0	50.0	54.2	57.7	4.4	0.0	4.081798	1683	3218	1709-1709	1.000000	47.6
rps16_p1	rps16	1	ACAGCGAGGGATACTAGTGA	TCCCTATACCACTTTGCAATGA	20	22	50.0	40.9	57.3	57.1	5.4	18.3	3.584402	4519	5645	1201-1224	1.000000	52.8
rps16_p2	rps16	2	AGACAGCGAGGGATACTAGT	TCCCTATACCACTTTGCAATGA	20	22	50.0	40.9	57.0	57.1	0.0	18.3	3.887328	4519	5645	1203-1226	1.000000	50.4
rps16_p3	rps16	3	ACAGCGAGGGATACTAGTGA	TCAATTCCCTATACCACTTTGCA	20	23	50.0	39.1	57.3	57.8	5.4	0.0	3.917190	4519	5645	1206-1229	1.000000	50.2
rps16_p4	rps16	4	ACAGCGAGGGATACTAGTGA	TCCCTATACCACTTTGCAATGAA	20	23	50.0	39.1	57.3	57.8	5.4	18.3	3.917190	4519	5645	1201-1224	1.000000	50.2
rps16_p5	rps16	5	ACAGCGAGGGATACTAGTGA	TTCCCTATACCACTTTGCAATGA	20	23	50.0	39.1	57.3	57.8	5.4	18.3	3.917190	4519	5645	1202-1225	1.000000	50.2
psbK-psbI_p1	psbK-psbI	1	CGTGGATGTTATGCCTGTCA	GGATTACGTCCTGGGTCATT	20	20	50.0	50.0	58.0	57.0	0.0	0.0	1.025221	7316	7714	573-579	1.000000	72.8
psbK-psbI_p2	psbK-psbI	2	GTTTGGCAAGCTGCTGTAAG	GGATTACGTCCTGGGTCATT	20	20	50.0	50.0	57.9	57.0	1.4	0.0	1.058551	7316	7714	524-530	1.000000	72.6
psbK-psbI_p3	psbK-psbI	3	TGGCAAGCTGCTGTAAGTTT	GGATTACGTCCTGGGTCATT	20	20	45.0	50.0	58.3	57.0	1.4	0.0	1.311260	7316	7714	521-527	1.000000	70.6
psbK-psbI_p4	psbK-psbI	4	TTGGCAAGCTGCTGTAAGTT	GGATTACGTCCTGGGTCATT	20	20	45.0	50.0	58.3	57.0	1.4	0.0	1.311260	7316	7714	522-528	1.000000	70.6
psbK-psbI_p5	psbK-psbI	5	TTTGGCAAGCTGCTGTAAGT	GGATTACGTCCTGGGTCATT	20	20	45.0	50.0	58.3	57.0	1.4	0.0	1.311260	7316	7714	523-529	1.000000	70.6
psbZ-trnfM-CAU_p1	psbZ-trnfM-CAU	1	TTGCTTCTCCTGATGGTTGG	CGAGGCTCATAACCTTGAGG	20	20	50.0	55.0	57.8	57.8	0.0	16.0	0.436237	12216	13001	943-948	1.000000	77.9
psbZ-trnfM-CAU_p2	psbZ-trnfM-CAU	2	GCTTCTCCTGATGGTTGGTC	CGAGGCTCATAACCTTGAGG	20	20	55.0	55.0	58.3	57.8	0.0	16.0	0.487219	12216	13001	941-946	1.000000	77.5
psbZ-trnfM-CAU_p3	psbZ-trnfM-CAU	3	TTGCTTCTCCTGATGGTTGG	CAGTTTGGTAGCTCACGAGG	20	20	50.0	55.0	57.8	58.3	0.0	0.0	0.490228	12216	13001	958-963	1.000000	77.5
psbZ-trnfM-CAU_p4	psbZ-trnfM-CAU	4	GCTTCTCCTGATGGTTGGTC	CAGTTTGGTAGCTCACGAGG	20	20	55.0	55.0	58.3	58.3	0.0	0.0	0.541210	12216	13001	956-961	1.000000	77.1
psbZ-trnfM-CAU_p5	psbZ-trnfM-CAU	5	TTGCTTCTCCTGATGGTTGG	GAGCGGAGTAGAGCAGTTTG	20	20	50.0	55.0	57.8	58.4	0.0	0.0	0.570320	12216	13001	971-976	1.000000	76.8
trnT-GGU_p1	trnT-GGU	1	AGCCCTTTTAACTCAGTGGT	ACCAAAAATGCATGCATCCC	20	20	45.0	45.0	56.7	57.6	0.0	32.3	1.754965	15976	16042	247-247	1.000000	66.6
trnT-GGU_p2	trnT-GGU	2	AGGCATAAGTCATCGGTTCA	ACCAAAAATGCATGCATCCC	20	20	45.0	45.0	56.6	57.6	0.0	32.3	1.778292	15976	16042	210-210	1.000000	66.5
trnT-GGU_p3	trnT-GGU	3	AGCCCTTTTAACTCAGTGGT	GCATCCCGCACTAAGTCATA	20	20	45.0	50.0	56.7	57.5	0.0	0.0	1.874673	15976	16042	234-234	1.000000	65.7
trnT-GGU_p4	trnT-GGU	4	TGGTAAGGCATAAGTCATCGG	ACCAAAAATGCATGCATCCC	21	20	47.6	45.0	57.5	57.6	0.0	32.3	1.875604	15976	16042	215-215	1.000000	65.7
trnT-GGU_p5	trnT-GGU	5	GGTAAGGCATAAGTCATCGGT	ACCAAAAATGCATGCATCCC	21	20	47.6	45.0	57.5	57.6	0.0	32.3	1.877240	15976	16042	214-214	1.000000	65.7
psbM-petN_p1	psbM-petN	1	TGCTACTGCACTGTTCATTCT	TAGTAAGTCTCGCTTGGGCT	21	20	42.9	50.0	57.6	58.2	0.0	0.0	1.558772	18312	19078	898-929	1.000000	70.3
psbM-petN_p2	psbM-petN	2	TGCTACTGCACTGTTCATTCT	GTAGTATGGGGGAGGAGTGG	21	20	42.9	60.0	57.6	58.3	0.0	0.0	1.689144	18312	19078	842-873	1.000000	69.5
psbM-petN_p3	psbM-petN	3	TGCTACTGCACTGTTCATTCT	TAGTATGGGGGAGGAGTGGA	21	20	42.9	55.0	57.6	58.4	0.0	0.0	1.779576	18312	19078	841-872	1.000000	68.8
psbM-petN_p4	psbM-petN	4	TGCTACTGCACTGTTCATTC	TAGTAAGTCTCGCTTGGGCT	20	20	45.0	50.0	56.1	58.2	0.0	0.0	2.081320	18312	19078	898-929	1.000000	66.2
psbM-petN_p5	psbM-petN	5	TGCTACTGCACTGTTCATTCT	AGTAGTATGGGGGAGGAGTG	21	20	42.9	55.0	57.6	57.2	0.0	0.0	2.184882	18312	19078	843-874	1.000000	65.5
rps2_p1	rps2	1	CCGTAATGGAATGGTAGGATGA	AAATCTACGCAGTTGCCCAA	22	20	45.5	45.0	57.4	58.1	0.0	0.0	2.712966	31652	32362	810-810	1.000000	59.0
rps2_p2	rps2	2	CCGTAATGGAATGGTAGGATGA	ACGCAGTTGCCCAAGAATTA	22	20	45.5	45.0	57.4	58.1	0.0	0.0	2.712966	31652	32362	804-804	1.000000	59.0
rps2_p3	rps2	3	CCGTAATGGAATGGTAGGATGA	TACGCAGTTGCCCAAGAATT	22	20	45.5	45.0	57.4	58.1	0.0	0.0	2.712966	31652	32362	805-805	1.000000	59.0
rps2_p4	rps2	4	TCCGTAATGGAATGGTAGGATG	ACGCAGTTGCCCAAGAATTA	22	20	45.5	45.0	57.4	58.1	0.0	0.0	2.712966	31652	32362	805-805	1.000000	59.0
rps2_p5	rps2	5	TCCGTAATGGAATGGTAGGATG	TACGCAGTTGCCCAAGAATT	22	20	45.5	45.0	57.4	58.1	0.0	0.0	2.712966	31652	32362	806-806	1.000000	59.0
atpF-atpA_p1	atpF-atpA	1	GTGCAAGGAGCTCTAGGAAC	CATCCCCCACTTGAACTACG	20	20	55.0	55.0	58.0	58.0	14.6	0.0	0.041314	36266	36357	312-312	1.000000	80.2
atpF-atpA_p2	atpF-atpA	2	TGCAAGGAGCTCTAGGAACT	CATCCCCCACTTGAACTACG	20	20	50.0	55.0	58.1	58.0	14.6	0.0	0.079998	36266	36357	311-311	1.000000	79.9
atpF-atpA_p3	atpF-atpA	3	GTGCAAGGAGCTCTAGGAAC	TTGTTCAATACGTTCGCGGA	20	20	55.0	45.0	58.0	58.2	14.6	6.1	0.236332	36266	36357	257-257	1.000000	78.7
atpF-atpA_p4	atpF-atpA	4	TGCAAGGAGCTCTAGGAACT	TTGTTCAATACGTTCGCGGA	20	20	50.0	45.0	58.1	58.2	14.6	6.1	0.275017	36266	36357	256-256	1.000000	78.4
atpF-atpA_p5	atpF-atpA	5	ATTTCCGTACGATTCGTGCT	CATCCCCCACTTGAACTACG	20	20	45.0	55.0	57.7	58.0	21.4	0.0	0.313136	36266	36357	263-263	1.000000	78.1
trnfM-CAU_p1	trnfM-CAU	1	AGAAGGAATATGGAGCGGGT	CCCCGCTCTTTCGTTTAAGA	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.281136	38237	38293	103-103	1.000000	78.5
trnfM-CAU_p2	trnfM-CAU	2	AGAAGGAATATGGAGCGGGT	TCCCCGCTCTTTCGTTTAAG	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.281136	38237	38293	104-104	1.000000	78.5
trnfM-CAU_p3	trnfM-CAU	3	AGAAGGAATATGGAGCGGGT	GTTCCCCGCTCTTTCGTTTA	20	20	50.0	50.0	58.2	58.2	0.0	0.0	0.392643	38237	38293	106-106	1.000000	77.6
trnfM-CAU_p4	trnfM-CAU	4	AGAAGGAATATGGAGCGGGT	TATGGTAGAGGGGCGTATCC	20	20	50.0	55.0	58.2	57.8	0.0	0.0	0.419148	38237	38293	145-145	1.000000	77.2
trnfM-CAU_p5	trnfM-CAU	5	AGGAATATGGAGCGGGTAGT	CCCCGCTCTTTCGTTTAAGA	20	20	50.0	50.0	57.6	57.9	0.0	0.0	0.489416	38237	38293	100-100	1.000000	76.9
psaA-ycf3_p1	psaA-ycf3	1	TGTTCTTGAGAAATGGCCGG	TATAGCGCTTACTCCGGGAA	20	20	50.0	50.0	58.5	58.0	0.0	18.7	0.480902	43413	44026	795-795	1.000000	77.1
psaA-ycf3_p2	psaA-ycf3	2	TTTTACTTCTGGTTCCGGCG	TATAGCGCTTACTCCGGGAA	20	20	50.0	50.0	58.5	58.0	0.0	18.7	0.499445	43413	44026	723-723	1.000000	77.0
psaA-ycf3_p3	psaA-ycf3	3	TGTTCTTGAGAAATGGCCGG	GCGCTTACTCCGGGAAATTA	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.522343	43413	44026	791-791	1.000000	76.7
psaA-ycf3_p4	psaA-ycf3	4	TTTTACTTCTGGTTCCGGCG	GCGCTTACTCCGGGAAATTA	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.540887	43413	44026	719-719	1.000000	76.7
psaA-ycf3_p5	psaA-ycf3	5	GGTTTGGCCCATTCCTCAAA	TATAGCGCTTACTCCGGGAA	20	20	50.0	50.0	58.7	58.0	0.0	18.7	0.667465	43413	44026	776-776	1.000000	75.6
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	ATTTGAACCGATGACCCTCG	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032006	47941	48698	858-859	1.000000	80.3
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	CGATGACCCTCGCATTACAA	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.073242	47941	48698	850-851	1.000000	80.0
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.140570	47941	48698	828-829	1.000000	79.5
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	ATTTGAACCGATGACCCTCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	58.0	58.2	0.0	10.2	0.272624	47941	48698	848-849	1.000000	78.4
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	ATTTGAACCGATGACCCTCG	CAATCAAGTCCGTAGCGTCT	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.303758	47941	48698	857-858	1.000000	78.1
trnL-UAA-trnF-GAA_p1	trnL-UAA-trnF-GAA	1	AAGTCGTGAGGGTTCAAGTC	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.5	58.1	0.0	20.1	0.579937	49323	49677	445-446	1.000000	75.9
trnL-UAA-trnF-GAA_p2	trnL-UAA-trnF-GAA	2	AAGTCGTGAGGGTTCAAGTC	CTGTGCCAGGAACCAGATTT	20	20	50.0	50.0	57.5	58.1	0.0	0.0	0.612900	49323	49677	462-463	1.000000	75.6
trnL-UAA-trnF-GAA_p3	trnL-UAA-trnF-GAA	3	AAGTCGTGAGGGTTCAAGTC	GTGCCAGGAACCAGATTTGA	20	20	50.0	50.0	57.5	58.1	0.0	0.0	0.618341	49323	49677	460-461	1.000000	75.6
trnL-UAA-trnF-GAA_p4	trnL-UAA-trnF-GAA	4	AAGTCGTGAGGGTTCAAGTC	TTTTTCTGTGCCAGGAACCA	20	20	50.0	45.0	57.5	57.8	0.0	0.0	0.679543	49323	49677	467-468	1.000000	75.6
trnL-UAA-trnF-GAA_p5	trnL-UAA-trnF-GAA	5	AAGTCGTGAGGGTTCAAGTC	TTTTCTGTGCCAGGAACCAG	20	20	50.0	50.0	57.5	58.3	0.0	0.0	0.836845	49323	49677	466-467	1.000000	74.3
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CTAACCGGGGCTAAAAGTCC	GGTTCGAATCCGTATAGCCC	20	20	55.0	55.0	58.0	57.9	6.3	0.0	0.110083	52016	52900	1011-1021	1.000000	79.7
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CTAACCGGGGCTAAAAGTCC	CGGTTCGAATCCGTATAGCC	20	20	55.0	55.0	58.0	58.3	6.3	22.8	0.339465	52016	52900	1012-1022	1.000000	77.9
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TAACCGGGGCTAAAAGTCCT	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	58.3	57.9	6.3	0.0	0.415737	52016	52900	1010-1020	1.000000	77.3
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CACTAACCGGGGCTAAAAGT	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	57.5	57.9	6.3	0.0	0.560721	52016	52900	1013-1023	1.000000	76.1
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TAACCGGGGCTAAAAGTCCT	CGGTTCGAATCCGTATAGCC	20	20	50.0	55.0	58.3	58.3	6.3	22.8	0.645119	52016	52900	1011-1021	1.000000	75.5
rbcL-psaI_p1	rbcL-psaI	1	CGCAGCTTGTGAAGTATGGA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.3	58.0	0.0	26.9	0.301363	58056	59346	1428-1434	1.000000	77.9
rbcL-psaI_p2	rbcL-psaI	2	CGCAGCTTGTGAAGTATGGA	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.3	57.9	0.0	26.8	0.377897	58056	59346	1434-1440	1.000000	77.3
rbcL-psaI_p3	rbcL-psaI	3	GGCGATCAAATTCGAGTTCG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.6	58.0	0.0	26.9	0.380261	58056	59346	1407-1413	1.000000	77.2
rbcL-psaI_p4	rbcL-psaI	4	TGGAAGGCGATCAAATTCGA	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	57.6	58.0	0.0	26.9	0.421933	58056	59346	1412-1418	1.000000	76.9
rbcL-psaI_p5	rbcL-psaI	5	GGCGATCAAATTCGAGTTCG	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	57.6	57.9	0.0	26.8	0.456795	58056	59346	1413-1419	1.000000	76.6
petA-psbJ_p1	petA-psbJ	1	GCGTCCAAGGCCTTTTATTC	TCCTCTTTGGCTGATAGGTACT	20	22	50.0	45.5	57.4	58.0	17.6	0.0	2.566098	62712	63699	1197-1198	1.000000	59.7
petA-psbJ_p2	petA-psbJ	2	GCGTCCAAGGCCTTTTATTC	CCTCTTTGGCTGATAGGTACTG	20	22	50.0	50.0	57.4	57.9	17.6	0.0	2.627055	62712	63699	1196-1197	1.000000	59.3
petA-psbJ_p3	petA-psbJ	3	GCGTCCAAGGCCTTTTATTC	TTCCTCTTTGGCTGATAGGT	20	20	50.0	45.0	57.4	55.8	17.6	0.0	2.739592	62712	63699	1198-1199	1.000000	58.4
petA-psbJ_p4	petA-psbJ	4	GCGTCCAAGGCCTTTTATTC	ACGGTTCATATTCTGGATTGGG	20	22	50.0	45.5	57.4	58.2	17.6	0.0	2.741176	62712	63699	1132-1133	1.000000	58.4
petA-psbJ_p5	petA-psbJ	5	GCGTCCAAGGCCTTTTATTC	CGGTTCATATTCTGGATTGGGT	20	22	50.0	45.5	57.4	58.2	17.6	0.0	2.741176	62712	63699	1131-1132	1.000000	58.4
psbE-petL_p1	psbE-petL	1	TGACAAATAACCAACCCGCA	AAGCAGCCAGTAGAAAACCG	20	20	45.0	50.0	58.0	58.5	0.0	0.0	0.493582	64469	65710	1377-1400	1.000000	77.4
psbE-petL_p2	psbE-petL	2	TGACAAATAACCAACCCGCA	GCAGCCAGTAGAAAACCGAA	20	20	45.0	50.0	58.0	58.5	0.0	0.0	0.497931	64469	65710	1375-1398	1.000000	77.3
psbE-petL_p3	psbE-petL	3	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	20	20	50.0	50.0	57.9	58.5	6.4	0.0	0.527577	64469	65710	1366-1389	1.000000	77.1
psbE-petL_p4	psbE-petL	4	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	20	20	50.0	50.0	57.9	58.5	6.4	0.0	0.531926	64469	65710	1364-1387	1.000000	77.1
psbE-petL_p5	psbE-petL	5	ACCAACCCGCAATGAATAGG	AAGCAGCCAGTAGAAAACCG	20	20	50.0	50.0	58.2	58.5	0.0	0.0	0.713535	64469	65710	1368-1391	1.000000	75.6
ndhH_p1	ndhH	1	ATCCCGTGAAATTCTCGAGC	GCCCCTTATTCCCCTTCCTA	20	20	50.0	55.0	58.1	58.2	17.5	0.0	0.229537	102946	103167	1002-1060	1.000000	78.6
ndhH_p2	ndhH	2	ATCCCGTGAAATTCTCGAGC	TAGGGGGAGGACGAATATCG	20	20	50.0	55.0	58.1	57.8	17.5	0.0	0.269925	102946	103167	1115-1173	1.000000	78.3
ndhH_p3	ndhH	3	TGCATTTTGATGAGCCTCGT	GCCCCTTATTCCCCTTCCTA	20	20	45.0	55.0	58.2	58.2	0.0	0.0	0.345772	102946	103167	348-406	1.000000	78.3
ndhH_p4	ndhH	4	TGCATTTTGATGAGCCTCGT	TAGGGGGAGGACGAATATCG	20	20	45.0	55.0	58.2	57.8	0.0	0.0	0.386160	102946	103167	461-519	1.000000	77.7
ndhH_p5	ndhH	5	ATTCTCGAGCCGAAAGATGG	GCCCCTTATTCCCCTTCCTA	20	20	50.0	55.0	57.8	58.2	7.8	0.0	0.402077	102946	103167	992-1050	1.000000	77.2