primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
APL01_gp032_p1	APL01_gp032	1	CAATCCACTGCCTTGATCCA	GAGGTATTGAAGGTAAAGGAGCA	20	23	50.0	43.5	57.9	57.9	0.0	0.0	3.232324	156	275	331-338	1.000000	58.2
APL01_gp032_p2	APL01_gp032	2	AATCCACTGCCTTGATCCAC	GAGGTATTGAAGGTAAAGGAGCA	20	23	50.0	43.5	57.9	57.9	0.0	0.0	3.233831	156	275	330-337	1.000000	58.2
APL01_gp032_p3	APL01_gp032	3	ACAATCCACTGCCTTGATCC	GAGGTATTGAAGGTAAAGGAGCA	20	23	50.0	43.5	57.9	57.9	0.0	0.0	3.233831	156	275	332-339	1.000000	58.2
APL01_gp032_p4	APL01_gp032	4	CTTGATCCACTTGGCCACAT	GAGGTATTGAAGGTAAAGGAGCA	20	23	50.0	43.5	58.2	57.9	3.9	0.0	3.255674	156	275	320-327	1.000000	58.2
APL01_gp032_p5	APL01_gp032	5	TTGATCCACTTGGCCACATC	GAGGTATTGAAGGTAAAGGAGCA	20	23	50.0	43.5	58.2	57.9	3.9	0.0	3.260930	156	275	319-326	1.000000	58.1
APL01_gt002-APL01_gp072_p1	APL01_gt002-APL01_gp072	1	CGAATCCCTTGCTTCATCCA	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.0	58.0	0.0	7.6	0.067026	4275	5063	947-951	1.000000	80.9
APL01_gt002-APL01_gp072_p2	APL01_gt002-APL01_gp072	2	ACGAATCCCTTGCTTCATCC	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.0	58.0	0.0	7.6	0.068463	4275	5063	948-952	1.000000	80.9
APL01_gt002-APL01_gp072_p3	APL01_gt002-APL01_gp072	3	ATGGACGAATCCCTTGCTTC	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.0	58.0	0.0	7.6	0.068463	4275	5063	952-956	1.000000	80.9
APL01_gt002-APL01_gp072_p4	APL01_gt002-APL01_gp072	4	GGACGAATCCCTTGCTTCAT	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.0	58.0	0.0	7.6	0.068463	4275	5063	950-954	1.000000	80.9
APL01_gt002-APL01_gp072_p5	APL01_gt002-APL01_gp072	5	CGATGGTATGGACGAATCCC	AAAAAGGCGCTCAACCTACA	20	20	55.0	45.0	57.9	58.0	0.0	7.6	0.104626	4275	5063	959-963	1.000000	80.6
APL01_gt004-APL01_gt005_p1	APL01_gt004-APL01_gt005	1	CAATCCACCGCTTTAGTCCA	ACGAATCGCACTTTTACCACT	20	21	50.0	42.9	57.9	58.2	0.0	1.2	1.345205	9115	9674	629-630	1.000000	69.9
APL01_gt004-APL01_gt005_p2	APL01_gt004-APL01_gt005	2	AATCCACCGCTTTAGTCCAC	ACGAATCGCACTTTTACCACT	20	21	50.0	42.9	57.9	58.2	0.0	1.2	1.346679	9115	9674	628-629	1.000000	69.9
APL01_gt004-APL01_gt005_p3	APL01_gt004-APL01_gt005	3	CAATCCACCGCTTTAGTCCA	ACGAATCGCACTTTTACCAC	20	20	50.0	45.0	57.9	56.8	0.0	1.2	1.352230	9115	9674	629-630	1.000000	69.8
APL01_gt004-APL01_gt005_p4	APL01_gt004-APL01_gt005	4	AATCCACCGCTTTAGTCCAC	ACGAATCGCACTTTTACCAC	20	20	50.0	45.0	57.9	56.8	0.0	1.2	1.353705	9115	9674	628-629	1.000000	69.8
APL01_gt004-APL01_gt005_p5	APL01_gt004-APL01_gt005	5	CTCGTACAGCGGATTAGCAA	ACGAATCGCACTTTTACCACT	20	21	50.0	42.9	57.8	58.2	0.0	1.2	1.441055	9115	9674	646-647	1.000000	69.1
APL01_gp069-APL01_gp068_p1	APL01_gp069-APL01_gp068	1	ATGCCATCGCCTACTTGAAG	TGTTTGGGGCGATGAAAGAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.052665	12464	12514	199-200	1.000000	80.0
APL01_gp069-APL01_gp068_p2	APL01_gp069-APL01_gp068	2	ATGCCATCGCCTACTTGAAG	ACAAGGGGCTTTAGCAACTC	20	20	50.0	50.0	58.0	58.1	0.0	3.9	0.129901	12464	12514	276-277	1.000000	79.3
APL01_gp069-APL01_gp068_p3	APL01_gp069-APL01_gp068	3	ATGCCATCGCCTACTTGAAG	GGGGCTTTAGCAACTCTGAA	20	20	50.0	50.0	58.0	57.8	0.0	3.9	0.241331	12464	12514	272-273	1.000000	78.4
APL01_gp069-APL01_gp068_p4	APL01_gp069-APL01_gp068	4	ATGCCATCGCCTACTTGAAG	AAGGGGCTTTAGCAACTCTG	20	20	50.0	50.0	58.0	57.8	0.0	3.9	0.247535	12464	12514	274-275	1.000000	78.3
APL01_gp069-APL01_gp068_p5	APL01_gp069-APL01_gp068	5	ATGCCATCGCCTACTTGAAG	CAAGGGGCTTTAGCAACTCT	20	20	50.0	50.0	58.0	57.8	0.0	3.9	0.247535	12464	12514	275-276	1.000000	78.3
APL01_gp067-APL01_gp066_p1	APL01_gp067-APL01_gp066	1	TACCTTCTACAGCTTGGCCT	GCGAATCTTTGGAGGGTCAT	20	20	50.0	50.0	58.0	58.0	3.3	0.0	0.091501	14212	15286	1216-1242	1.000000	80.5
APL01_gp067-APL01_gp066_p2	APL01_gp067-APL01_gp066	2	CAGTACCTTGACCAACTCCG	GCGAATCTTTGGAGGGTCAT	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.124409	14212	15286	1192-1218	1.000000	80.3
APL01_gp067-APL01_gp066_p3	APL01_gp067-APL01_gp066	3	AACGGAAGCAGCAGAAATCA	GCGAATCTTTGGAGGGTCAT	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.147903	14212	15286	1131-1157	1.000000	80.1
APL01_gp067-APL01_gp066_p4	APL01_gp067-APL01_gp066	4	TACCTTCTACAGCTTGGCCT	TTTTGCAACTTTAGCTGCGG	20	20	50.0	45.0	58.0	57.9	3.3	0.0	0.180119	14212	15286	1245-1271	1.000000	79.8
APL01_gp067-APL01_gp066_p5	APL01_gp067-APL01_gp066	5	AGCCAATCCAGCAGCAATAA	GCGAATCTTTGGAGGGTCAT	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.192568	14212	15286	1149-1175	1.000000	79.8
APL01_gp042-APL01_gp041_p1	APL01_gp042-APL01_gp041	1	TTCGGATTTGATTGTGTCGT	ACGGACTAGCTGTACCTACC	20	20	40.0	55.0	55.7	58.0	0.0	0.0	2.352203	64496	64517	110-110	1.000000	61.9
APL01_gp042-APL01_gp041_p2	APL01_gp042-APL01_gp041	2	TTCGGATTTGATTGTGTCGT	GGACTAGCTGTACCTACCGT	20	20	40.0	55.0	55.7	58.0	0.0	0.0	2.352203	64496	64517	108-108	1.000000	61.9
APL01_gp042-APL01_gp041_p3	APL01_gp042-APL01_gp041	3	CATTCGGATTTGATTGTGTCGT	ACGGACTAGCTGTACCTACC	22	20	40.9	55.0	57.7	58.0	0.0	0.0	2.390783	64496	64517	112-112	1.000000	61.6
APL01_gp042-APL01_gp041_p4	APL01_gp042-APL01_gp041	4	CATTCGGATTTGATTGTGTCGT	GGACTAGCTGTACCTACCGT	22	20	40.9	55.0	57.7	58.0	0.0	0.0	2.390783	64496	64517	110-110	1.000000	61.6
APL01_gp042-APL01_gp041_p5	APL01_gp042-APL01_gp041	5	TTCGGATTTGATTGTGTCGT	ATGGTTAGCTGTTCACGGAC	20	20	40.0	50.0	55.7	57.9	0.0	0.0	2.397077	64496	64517	124-124	1.000000	61.5
APL01_gp033_p1	APL01_gp033	1	CATGGGTCGAGAACGGAAAT	CCTTGTTCCTACATGACCCG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.048351	122676	122795	1453-1455	1.000000	81.8
APL01_gp033_p2	APL01_gp033	2	TCCACAGTCCTGAGGCTAAA	CCTTGTTCCTACATGACCCG	20	20	50.0	55.0	58.0	58.0	6.0	0.0	0.048351	122676	122795	1144-1151	1.000000	81.8
APL01_gp033_p3	APL01_gp033	3	TTCCACAGTCCTGAGGCTAA	CCTTGTTCCTACATGACCCG	20	20	50.0	55.0	58.0	58.0	6.0	0.0	0.048351	122676	122795	1145-1152	1.000000	81.8
APL01_gp033_p4	APL01_gp033	4	TGAGATCAAATCCACCGCTG	CCTTGTTCCTACATGACCCG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.052595	122676	122795	1423-1425	1.000000	81.8
APL01_gp033_p5	APL01_gp033	5	GAGATCAAATCCACCGCTGT	CCTTGTTCCTACATGACCCG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.054014	122676	122795	1422-1424	1.000000	81.8
APL01_gp020-APL01_gp019_p1	APL01_gp020-APL01_gp019	1	CCGCTATTGTCCGCTACATT	AATAGCGGCGTCAAAAATGC	20	20	50.0	45.0	58.1	58.1	0.0	9.4	0.226070	81255	81375	217-224	1.000000	80.4
APL01_gp020-APL01_gp019_p2	APL01_gp020-APL01_gp019	2	TCCGCTACATTCAAATGGGT	AATAGCGGCGTCAAAAATGC	20	20	45.0	45.0	57.2	58.1	7.6	9.4	0.891060	81255	81375	208-215	1.000000	75.1
APL01_gp020-APL01_gp019_p3	APL01_gp020-APL01_gp019	3	CCGCTATTGTCCGCTACATT	CATTTCAATAGCGGCGTCAA	20	20	50.0	45.0	58.1	57.2	0.0	9.4	0.957331	81255	81375	223-230	1.000000	74.5
APL01_gp020-APL01_gp019_p4	APL01_gp020-APL01_gp019	4	TACACATCAACTCTCGAGCC	AATAGCGGCGTCAAAAATGC	20	20	50.0	45.0	57.1	58.1	7.8	9.4	1.033388	81255	81375	237-244	1.000000	73.7
APL01_gp020-APL01_gp019_p5	APL01_gp020-APL01_gp019	5	CCGCTATTGTCCGCTACATT	CCATTTCAATAGCGGCGTCA	20	20	50.0	50.0	58.1	59.0	0.0	9.4	1.120539	81255	81375	224-231	1.000000	73.1
APL01_gp013-APL01_gp012_p1	APL01_gp013-APL01_gp012	1	ATGGCTTTTCCCGGATGAAA	AACAGGTGTTTCCACGACTC	20	20	45.0	50.0	57.8	58.1	0.7	0.0	0.280664	93516	93595	145-150	1.000000	81.0
APL01_gp013-APL01_gp012_p2	APL01_gp013-APL01_gp012	2	TGGCTTTTCCCGGATGAAAT	AACAGGTGTTTCCACGACTC	20	20	45.0	50.0	57.8	58.1	0.7	0.0	0.280664	93516	93595	144-149	1.000000	81.0
APL01_gp013-APL01_gp012_p3	APL01_gp013-APL01_gp012	3	AAGATGGCTTTTCCCGGATG	AACAGGTGTTTCCACGACTC	20	20	50.0	50.0	58.2	58.1	0.7	0.0	0.282776	93516	93595	148-153	1.000000	80.9
APL01_gp013-APL01_gp012_p4	APL01_gp013-APL01_gp012	4	GATGGCTTTTCCCGGATGAA	AACAGGTGTTTCCACGACTC	20	20	50.0	50.0	58.2	58.1	0.7	0.0	0.287827	93516	93595	146-151	1.000000	80.9
APL01_gp013-APL01_gp012_p5	APL01_gp013-APL01_gp012	5	GTTCCTATCTAACGGAGCGC	AACAGGTGTTTCCACGACTC	20	20	55.0	50.0	58.2	58.1	0.0	0.0	0.282025	93516	93595	202-207	1.000000	80.1
APL01_gp010-APL01_gt028_p1	APL01_gp010-APL01_gt028	1	ACGATCGAAAAAGAGATACGAGT	GTGAAATCGGTAGACACGCT	23	20	39.1	50.0	57.8	58.0	24.3	0.0	3.240440	109627	109722	220-220	1.000000	55.9
APL01_gp010-APL01_gt028_p2	APL01_gp010-APL01_gt028	2	ACGATCGAAAAAGAGATACGAGT	TGAAATCGGTAGACACGCTG	23	20	39.1	50.0	57.8	58.0	24.3	0.0	3.241811	109627	109722	219-219	1.000000	55.9
APL01_gp010-APL01_gt028_p3	APL01_gp010-APL01_gt028	3	ACGATCGAAAAAGAGATACGAGT	TGATTTCGAACAAGCCGCTA	23	20	39.1	45.0	57.8	57.9	24.3	0.0	3.320111	109627	109722	242-242	1.000000	55.1
APL01_gp010-APL01_gt028_p4	APL01_gp010-APL01_gt028	4	ACGATCGAAAAAGAGATACGAGT	AACAAGCCGCTATGGTGAAA	23	20	39.1	45.0	57.8	58.1	24.3	0.0	3.328323	109627	109722	234-234	1.000000	55.1
APL01_gp010-APL01_gt028_p5	APL01_gp010-APL01_gt028	5	ACGATCGAAAAAGAGATACGAGT	TAGACACGCTGCTCTTAGGA	23	20	39.1	50.0	57.8	57.9	24.3	0.0	3.356887	109627	109722	210-210	1.000000	55.0
APL01_gt028-APL01_gp009_p1	APL01_gt028-APL01_gp009	1	AGCGTGTCTACCGATTTCAC	TTGGACGAAGAAGGGGTACT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.027638	109803	110589	913-920	1.000000	81.2
APL01_gt028-APL01_gp009_p2	APL01_gt028-APL01_gp009	2	CAGCGTGTCTACCGATTTCA	TTGGACGAAGAAGGGGTACT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.029008	109803	110589	914-921	1.000000	81.2
APL01_gt028-APL01_gp009_p3	APL01_gt028-APL01_gp009	3	AGCGTGTCTACCGATTTCAC	GTTGGACGAAGAAGGGGTAC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.098230	109803	110589	914-921	1.000000	80.6
APL01_gt028-APL01_gp009_p4	APL01_gt028-APL01_gp009	4	CAGCGTGTCTACCGATTTCA	GTTGGACGAAGAAGGGGTAC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.099601	109803	110589	915-922	1.000000	80.6
APL01_gt028-APL01_gp009_p5	APL01_gt028-APL01_gp009	5	TCCTAAGAGCAGCGTGTCTA	TTGGACGAAGAAGGGGTACT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.144085	109803	110589	923-930	1.000000	80.2