primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GTG-psbA_p1	trnH-GTG-psbA	1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.161600	84	492	525-531	1.000000	79.5
trnH-GTG-psbA_p2	trnH-GTG-psbA	2	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.165962	84	492	539-545	1.000000	79.4
trnH-GTG-psbA_p3	trnH-GTG-psbA	3	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	84	492	538-544	1.000000	79.4
trnH-GTG-psbA_p4	trnH-GTG-psbA	4	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	84	492	540-546	1.000000	79.4
trnH-GTG-psbA_p5	trnH-GTG-psbA	5	TATTTATGGGCGAACGACGG	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.177167	84	492	586-592	1.000000	79.3
rps16_p1	rps16	1	GGGGGTTGTATAGAAAGGGT	TGTATCGACCGAACCAATGA	20	20	50.0	45.0	56.2	56.7	0.0	0.0	3.146177	5535	6666	1308-1332	1.000000	56.6
rps16_p2	rps16	2	GGGGGTTGTATAGAAAGGGTT	TGTATCGACCGAACCAATGA	21	20	47.6	45.0	57.0	56.7	0.0	0.0	3.365655	5535	6666	1308-1332	1.000000	54.9
rps16_p3	rps16	3	GGGGGTTGTATAGAAAGGGT	ATGTATCGACCGAACCAATGA	20	21	50.0	42.9	56.2	57.2	0.0	0.0	3.627886	5535	6666	1309-1333	1.000000	52.8
rps16_p4	rps16	4	GGGGGTTGTATAGAAAGGGT	TGTATCGACCGAACCAATGAT	20	21	50.0	42.9	56.2	57.2	0.0	0.0	3.627886	5535	6666	1308-1332	1.000000	52.8
rps16_p5	rps16	5	GGGGGTTGTATAGAAAGGGTT	TGTATCGACCGAACCAATGAT	21	21	47.6	42.9	57.0	57.2	0.0	0.0	3.847363	5535	6666	1308-1332	1.000000	51.0
trnS-GCT-trnR-TCT_p1	trnS-GCT-trnR-TCT	1	CAATCCGACGCTTTAGTCCA	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.056187	9576	11401	1897-1913	1.000000	65.5
trnS-GCT-trnR-TCT_p2	trnS-GCT-trnR-TCT	2	AATCCGACGCTTTAGTCCAC	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.057575	9576	11401	1896-1912	1.000000	65.5
trnS-GCT-trnR-TCT_p3	trnS-GCT-trnR-TCT	3	CAATCCGACGCTTTAGTCCA	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.139515	9576	11401	1903-1919	1.000000	64.9
trnS-GCT-trnR-TCT_p4	trnS-GCT-trnR-TCT	4	AATCCGACGCTTTAGTCCAC	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.140903	9576	11401	1902-1918	1.000000	64.8
trnS-GCT-trnR-TCT_p5	trnS-GCT-trnR-TCT	5	ACAAAAGCGGAAAGAGAGGG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.1	56.9	0.0	0.0	2.154045	9576	11401	1957-1973	1.000000	64.7
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	58.0	57.9	1.4	0.0	0.171127	15401	16614	1259-1301	1.000000	81.5
atpH-atpI_p2	atpH-atpI	2	GATCCCTTCTACAGCTTGGC	TTTTGCAACTTTAGCTGCGG	20	20	55.0	45.0	58.0	57.9	0.0	0.0	0.172323	15401	16614	1344-1386	1.000000	81.3
atpH-atpI_p3	atpH-atpI	3	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	58.2	57.9	1.4	0.0	0.316666	15401	16614	1260-1302	1.000000	80.3
atpH-atpI_p4	atpH-atpI	4	CGATCCCTTCTACAGCTTGG	TTTTGCAACTTTAGCTGCGG	20	20	55.0	45.0	57.8	57.9	0.0	0.0	0.363955	15401	16614	1345-1387	1.000000	79.8
atpH-atpI_p5	atpH-atpI	5	ATAGAAGCAAGACCGACAGC	TTTTGCAACTTTAGCTGCGG	20	20	50.0	45.0	57.7	57.9	0.0	0.0	0.434317	15401	16614	1292-1334	1.000000	79.3
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	58.2	57.6	0.0	0.0	1.558772	31183	32113	1093-1093	1.000000	68.0
petN-psbM_p2	petN-psbM	2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTC	20	20	50.0	45.0	58.2	56.1	0.0	0.0	2.081320	31183	32113	1093-1093	1.000000	63.8
petN-psbM_p3	petN-psbM	3	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.1	57.6	0.0	0.0	2.300208	31183	32113	1094-1094	1.000000	62.0
petN-psbM_p4	petN-psbM	4	CTCGCTTGGGCTGCTTTAAT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	58.9	57.6	0.0	0.0	2.309373	31183	32113	1085-1085	1.000000	62.0
petN-psbM_p5	petN-psbM	5	ATGGGGTAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.0	57.6	0.0	0.0	2.358413	31183	32113	1032-1032	1.000000	61.6
trnY-GTA-trnE-TTC_p1	trnY-GTA-trnE-TTC	1	TCTGGGGAACAACAAATGGG	CATAGTATGATGGCGGTCGG	20	20	50.0	55.0	58.0	58.0	0.0	3.9	0.011951	34023	34082	298-298	0.666667	66.6
trnY-GTA-trnE-TTC_p2	trnY-GTA-trnE-TTC	2	GGATTTGGATCTGTCGGGAC	CATAGTATGATGGCGGTCGG	20	20	55.0	55.0	58.0	58.0	0.0	3.9	0.048781	34023	34082	820-821	0.666667	66.6
trnY-GTA-trnE-TTC_p3	trnY-GTA-trnE-TTC	3	GCAAATGAACGGCGGTAAAA	CATAGTATGATGGCGGTCGG	20	20	45.0	55.0	57.9	58.0	13.2	3.9	0.056990	34023	34082	369-369	0.666667	66.6
trnY-GTA-trnE-TTC_p4	trnY-GTA-trnE-TTC	4	TTCCGATCAGGCATTTCTGG	CATAGTATGATGGCGGTCGG	20	20	50.0	55.0	58.0	58.0	0.0	3.9	0.046394	34023	34082	313-313	0.666667	66.3
trnY-GTA-trnE-TTC_p5	trnY-GTA-trnE-TTC	5	TCTGGGGAACAACAAATGGG	ATACTTGCCCCCATCGTCTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.105352	34023	34082	276-276	0.666667	65.8
trnE-TTC-trnT-GGT_p1	trnE-TTC-trnT-GGT	1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	34156	34978	920-926	1.000000	79.4
trnE-TTC-trnT-GGT_p2	trnE-TTC-trnT-GGT	2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	34156	34978	899-905	1.000000	79.3
trnE-TTC-trnT-GGT_p3	trnE-TTC-trnT-GGT	3	GCTGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	58.3	58.1	0.0	0.0	0.327456	34156	34978	899-905	1.000000	79.0
trnE-TTC-trnT-GGT_p4	trnE-TTC-trnT-GGT	4	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.7	58.1	0.0	0.0	0.345606	34156	34978	878-884	1.000000	78.9
trnE-TTC-trnT-GGT_p5	trnE-TTC-trnT-GGT	5	GCTGCCTCCTTGAAAGAGAG	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	58.3	58.1	0.0	0.0	0.349611	34156	34978	896-902	1.000000	78.8
psbC_p1	psbC	1	TCTTTGCGCCTAGTTTTGGT	GGTTGATAGCTCCGGTCTTG	20	20	45.0	55.0	58.0	58.1	4.2	0.0	0.083015	37162	38625	2255-2255	1.000000	82.0
psbC_p2	psbC	2	TGGTTCTTTGCGCCTAGTTT	GGTTGATAGCTCCGGTCTTG	20	20	45.0	55.0	58.0	58.1	4.2	0.0	0.083015	37162	38625	2259-2259	1.000000	82.0
psbC_p3	psbC	3	TTGGTTCTTTGCGCCTAGTT	GGTTGATAGCTCCGGTCTTG	20	20	45.0	55.0	58.0	58.1	4.2	0.0	0.083015	37162	38625	2260-2260	1.000000	82.0
psbC_p4	psbC	4	ACCGGTTTATGGATGAGTGC	GGTTGATAGCTCCGGTCTTG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.097062	37162	38625	1929-1929	1.000000	82.0
psbC_p5	psbC	5	CTTTGCGCCTAGTTTTGGTG	GGTTGATAGCTCCGGTCTTG	20	20	50.0	55.0	57.9	58.1	4.2	0.0	0.109152	37162	38625	2254-2254	1.000000	81.8
psaA-ycf3_p1	psaA-ycf3	1	TTGAGAAATGACCGGGTCTG	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	57.5	58.0	26.4	0.0	0.513654	45374	46146	971-972	1.000000	76.1
psaA-ycf3_p2	psaA-ycf3	2	TTTTACTTCTGGTTCCGGCG	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	58.5	58.0	0.0	0.0	0.525877	45374	46146	904-905	1.000000	76.1
psaA-ycf3_p3	psaA-ycf3	3	TTGAGAAATGACCGGGTCTG	CAGAGGCTTGGTTCGATCAA	20	20	50.0	50.0	57.5	57.9	26.4	0.0	0.571837	45374	46146	986-987	1.000000	75.7
psaA-ycf3_p4	psaA-ycf3	4	TTTTACTTCTGGTTCCGGCG	CAGAGGCTTGGTTCGATCAA	20	20	50.0	50.0	58.5	57.9	0.0	0.0	0.584060	45374	46146	919-920	1.000000	75.6
psaA-ycf3_p5	psaA-ycf3	5	TTGAGAAATGACCGGGTCTG	CGATCAAGCCGCTGAGTATT	20	20	50.0	50.0	57.5	58.1	26.4	0.0	0.614604	45374	46146	973-974	1.000000	75.3
trnT-TGT-trnF-GAA_p1	trnT-TGT-trnF-GAA	1	AAAGCCTGCTATCGGAATCG	TCTACCAGCTGAGCTATCCC	20	20	50.0	55.0	58.1	58.0	0.0	17.4	0.131425	49984	51774	1889-1892	1.000000	79.4
trnT-TGT-trnF-GAA_p2	trnT-TGT-trnF-GAA	2	GCGATGCTCTAACCTCTGAG	TCTACCAGCTGAGCTATCCC	20	20	55.0	55.0	57.9	58.0	0.0	17.4	0.135246	49984	51774	1844-1847	1.000000	79.4
trnT-TGT-trnF-GAA_p3	trnT-TGT-trnF-GAA	3	AAAGCCTGCTATCGGAATCG	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	58.1	58.1	0.0	20.1	0.180816	49984	51774	1925-1928	1.000000	79.0
trnT-TGT-trnF-GAA_p4	trnT-TGT-trnF-GAA	4	GCGATGCTCTAACCTCTGAG	TTTGAACTGGTGACACGAGG	20	20	55.0	50.0	57.9	58.1	0.0	20.1	0.184636	49984	51774	1880-1883	1.000000	79.0
trnT-TGT-trnF-GAA_p5	trnT-TGT-trnF-GAA	5	AAAGCCTGCTATCGGAATCG	GTGCCAGGAACCAGATTTGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.219220	49984	51774	1940-1943	1.000000	78.7
ndhC-trnM-CAT_p1	ndhC-trnM-CAT	1	GGGGCTAAAACTCCCGAAAT	CCCGCCGTATGAAAGCAATA	20	20	50.0	50.0	57.9	58.4	0.0	0.0	0.550649	54336	55756	1562-1563	1.000000	76.0
ndhC-trnM-CAT_p2	ndhC-trnM-CAT	2	CCTTTGCTAATCGGGGCTAA	CCCGCCGTATGAAAGCAATA	20	20	50.0	50.0	57.7	58.4	0.0	0.0	0.753172	54336	55756	1574-1575	1.000000	74.3
ndhC-trnM-CAT_p3	ndhC-trnM-CAT	3	GGGGCTAAAACTCCCGAAAT	CCGCCGTATGAAAGCAATAC	20	20	50.0	50.0	57.9	57.3	0.0	0.0	0.802602	54336	55756	1561-1562	1.000000	73.9
ndhC-trnM-CAT_p4	ndhC-trnM-CAT	4	GGGGCTAAAACTCCCGAAAT	TTTGAACCAATGACTCCCGC	20	20	50.0	50.0	57.9	58.8	0.0	0.0	0.895421	54336	55756	1577-1578	1.000000	73.2
ndhC-trnM-CAT_p5	ndhC-trnM-CAT	5	CTTCTCTGGCCCTTTGCTAA	CCCGCCGTATGAAAGCAATA	20	20	50.0	50.0	57.5	58.4	0.0	0.0	0.908224	54336	55756	1584-1585	1.000000	73.1
psaJ-rpl33_p1	psaJ-rpl33	1	TCCCGGATGCGTTGATATTC	AACATCTTTACCCTTGGCCA	20	20	50.0	45.0	57.8	57.0	0.7	0.0	1.154558	70955	71433	532-532	1.000000	71.1
psaJ-rpl33_p2	psaJ-rpl33	2	CCCGGATGCGTTGATATTCT	AACATCTTTACCCTTGGCCA	20	20	50.0	45.0	57.8	57.0	0.7	0.0	1.160572	70955	71433	531-531	1.000000	71.0
psaJ-rpl33_p3	psaJ-rpl33	3	TCCCGGATGCGTTGATATTC	AGCACCATTTCGATTAGCACT	20	21	50.0	42.9	57.8	58.0	0.7	0.0	1.172568	70955	71433	595-595	1.000000	70.9
psaJ-rpl33_p4	psaJ-rpl33	4	CCCGGATGCGTTGATATTCT	AGCACCATTTCGATTAGCACT	20	21	50.0	42.9	57.8	58.0	0.7	0.0	1.178582	70955	71433	594-594	1.000000	70.8
psaJ-rpl33_p5	psaJ-rpl33	5	TCCCGGATGCGTTGATATTC	GAACATCTTTACCCTTGGCCA	20	21	50.0	47.6	57.8	58.2	0.7	0.0	1.361099	70955	71433	533-533	1.000000	69.4
clpP_p1	clpP	1	GCATATTTGCGCGAAATCCC	CCCATTTTTACGCTTCCACA	20	20	50.0	45.0	58.6	56.6	15.3	0.0	1.976779	73824	75852	2106-2121	1.000000	64.9
clpP_p2	clpP	2	GCATATTTGCGCGAAATCCC	ACCCATTTTTACGCTTCCAC	20	20	50.0	45.0	58.6	56.6	15.3	0.0	1.979070	73824	75852	2107-2122	1.000000	64.9
clpP_p3	clpP	3	GCATATTTGCGCGAAATCCC	ACCCATTTTTACGCTTCCACA	20	21	50.0	42.9	58.6	58.4	15.3	0.0	2.002958	73824	75852	2107-2122	1.000000	64.7
clpP_p4	clpP	4	GCATATTTGCGCGAAATCCC	AACCCATTTTTACGCTTCCAC	20	21	50.0	42.9	58.6	57.3	15.3	0.0	2.245116	73824	75852	2108-2123	1.000000	62.8
clpP_p5	clpP	5	ATATTTGCGCGAAATCCCTG	CCCATTTTTACGCTTCCACA	20	20	45.0	45.0	56.9	56.6	15.3	0.0	2.450525	73824	75852	2104-2119	1.000000	61.1
trnN-GTT-ndhF_p1	trnN-GTT-ndhF	1	TGAACCTACGACCAGTCAGT	ATATAGGGGGTGGCCGAATT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.253679	112745	114417	1828-1830	1.000000	78.4
trnN-GTT-ndhF_p2	trnN-GTT-ndhF	2	ACGACCAGTCAGTTAACAGC	ATATAGGGGGTGGCCGAATT	20	20	50.0	50.0	57.8	58.2	8.6	0.0	0.395198	112745	114417	1821-1823	1.000000	77.2
trnN-GTT-ndhF_p3	trnN-GTT-ndhF	3	TGAACCTACGACCAGTCAGT	GGGTGGCCGAATTTCTTCTT	20	20	50.0	50.0	58.0	58.5	0.0	0.0	0.463038	112745	114417	1821-1823	1.000000	76.7
trnN-GTT-ndhF_p4	trnN-GTT-ndhF	4	TGAACCTACGACCAGTCAGT	TATAGGGGGTGGCCGAATTT	20	20	50.0	50.0	58.0	58.5	0.0	0.0	0.483704	112745	114417	1827-1829	1.000000	76.5
trnN-GTT-ndhF_p5	trnN-GTT-ndhF	5	ACGACCAGTCAGTTAACAGC	GGGTGGCCGAATTTCTTCTT	20	20	50.0	50.0	57.8	58.5	8.6	0.0	0.604556	112745	114417	1814-1816	1.000000	75.6
ndhA_p1	ndhA	1	TGACCCCAGTTACCATAGGG	CCCGCCGGGTTTTATCAATT	20	20	55.0	50.0	58.1	58.9	0.0	15.9	1.004258	124497	126793	2520-2536	1.000000	72.2
ndhA_p2	ndhA	2	TGACCCCAGTTACCATAGGG	CCGCCGGGTTTTATCAATTT	20	20	55.0	45.0	58.1	57.0	0.0	2.2	1.063660	124497	126793	2519-2535	1.000000	71.7
ndhA_p3	ndhA	3	GACCCCAGTTACCATAGGGA	CCCGCCGGGTTTTATCAATT	20	20	55.0	50.0	57.8	58.9	0.0	15.9	1.077718	124497	126793	2519-2535	1.000000	71.6
ndhA_p4	ndhA	4	GACCCCAGTTACCATAGGGA	CCGCCGGGTTTTATCAATTT	20	20	55.0	45.0	57.8	57.0	0.0	2.2	1.137120	124497	126793	2518-2534	1.000000	71.2
ndhA_p5	ndhA	5	TGACCCCAGTTACCATAGGG	GCCGGGTTTTATCAATTTGCA	20	21	55.0	42.9	58.1	58.0	0.0	2.2	1.146153	124497	126793	2517-2533	1.000000	71.1
ycf1_p1	ycf1	1	AGGGTCCGTTCAAGAAAGGA	ACGTATCCACCGAGTTTATCA	20	21	50.0	42.9	58.6	56.6	12.5	0.0	3.002310	133030	133179	280-280	1.000000	57.4
ycf1_p2	ycf1	2	AGGGTCCGTTCAAGAAAGGA	TCTTTTACGTATCCACCGAGT	20	21	50.0	42.9	58.6	56.5	12.5	0.0	3.070157	133030	133179	286-286	1.000000	56.8
ycf1_p3	ycf1	3	AGGGTCCGTTCAAGAAAGGA	ACGTATCCACCGAGTTTATCAA	20	22	50.0	40.9	58.6	57.3	12.5	0.0	3.293082	133030	133179	280-280	1.000000	55.1
ycf1_p4	ycf1	4	AGGGTCCGTTCAAGAAAGGA	TCTTTTACGTATCCACCGAGTT	20	22	50.0	40.9	58.6	57.2	12.5	0.0	3.358020	133030	133179	286-286	1.000000	54.5
ycf1_p5	ycf1	5	CGATAGGGTCCGTTCAAGAA	ACGTATCCACCGAGTTTATCA	20	21	50.0	42.9	57.0	56.6	0.0	0.0	3.386769	133030	133179	284-284	1.000000	54.3