primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	ATTAGCAATCCGCCGCTTTA	GGGGGTTACTCGAACGAATC	20	20	45.0	55.0	58.0	58.1	0.0	0.0	0.106300	8737	9290	610-645	1.000000	84.8
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	ACGGAAAGAGAGGGATTCGA	GGGGGTTACTCGAACGAATC	20	20	50.0	55.0	58.2	58.1	0.0	0.0	0.219442	8737	9290	659-694	1.000000	83.7
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	ATTAGCAATCCGCCGCTTTA	AGGGGGTTACTCGAACGAAT	20	20	45.0	50.0	58.0	58.4	0.0	0.0	0.473140	8737	9290	611-646	1.000000	81.8
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	GCTTTAGTCCACTCAGCCAT	GGGGGTTACTCGAACGAATC	20	20	50.0	55.0	57.6	58.1	0.9	0.0	0.478042	8737	9290	596-631	1.000000	81.8
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	AGTCCACTCAGCCATCTCTC	GGGGGTTACTCGAACGAATC	20	20	55.0	55.0	58.5	58.1	0.0	0.0	0.584056	8737	9290	591-626	1.000000	81.0
trnG-UCC_p1	trnG-UCC	1	ATTAGCAATCCGCCGCTTTA	CCCTCTTGCGGTAGAAGAAC	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.049747	9291	10062	1916-2008	1.000000	85.6
trnG-UCC_p2	trnG-UCC	2	ATTAGCAATCCGCCGCTTTA	GAGGAAGCAGAAGCCCTTTT	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.121701	9291	10062	1747-1839	1.000000	85.2
trnG-UCC_p3	trnG-UCC	3	GCGCAAATTATCCCTGTTCG	CCCTCTTGCGGTAGAAGAAC	20	20	50.0	55.0	57.9	58.0	0.3	0.0	0.116863	9291	10062	1408-1465	1.000000	85.1
trnG-UCC_p4	trnG-UCC	4	ATTAGCAATCCGCCGCTTTA	TTTACCGAGGAAGCAGAAGC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.132969	9291	10062	1753-1845	1.000000	85.1
trnG-UCC_p5	trnG-UCC	5	ACTCAGCCATCTCTCCCAAT	CCCTCTTGCGGTAGAAGAAC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.120988	9291	10062	1892-1984	1.000000	85.0
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	10063	10233	223-274	1.000000	73.2
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	10063	10233	226-277	1.000000	72.9
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	10063	10233	229-280	1.000000	72.4
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	10063	10233	232-283	1.000000	72.1
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.7	56.9	0.0	0.0	2.385293	10063	10233	227-278	1.000000	70.7
atpH-atpI_p1	atpH-atpI	1	CAGGATCCAGTCAGCAATCC	CATCTGCCTGCTATCGATCC	20	20	55.0	55.0	58.0	58.0	15.8	0.0	0.042808	16649	16768	1260-1297	0.250000	61.7
atpH-atpI_p2	atpH-atpI	2	GATCCAGTCAGCAATCCAGG	CATCTGCCTGCTATCGATCC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.042808	16649	16768	1257-1294	0.250000	61.7
atpH-atpI_p3	atpH-atpI	3	GGATCCAGTCAGCAATCCAG	CATCTGCCTGCTATCGATCC	20	20	55.0	55.0	58.0	58.0	7.0	0.0	0.042808	16649	16768	1258-1295	0.250000	61.7
atpH-atpI_p4	atpH-atpI	4	CTTGTTTCAATCGGCTGCTG	CATCTGCCTGCTATCGATCC	20	20	50.0	55.0	58.0	58.0	1.0	0.0	0.034222	16649	16768	803-803	0.125000	44.7
atpH-atpI_p5	atpH-atpI	5	TACCTAGCAAGCACAGCATG	CATCTGCCTGCTATCGATCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.035780	16649	16768	763-763	0.125000	44.7
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	33982	34685	728-760	1.000000	83.6
trnC-GCA-petN_p2	trnC-GCA-petN	2	CGATATGGCCGAGTGGTAAG	AGCCCAAGCGAGACTTACTA	20	20	55.0	50.0	57.9	58.2	0.0	0.0	0.217341	33982	34685	768-800	1.000000	83.5
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.432990	33982	34685	789-821	1.000000	81.7
trnC-GCA-petN_p4	trnC-GCA-petN	4	CGATATGGCCGAGTGGTAAG	AGAGTCCACTTCTTCCCCAT	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.416855	33982	34685	829-861	1.000000	81.6
trnC-GCA-petN_p5	trnC-GCA-petN	5	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	33982	34685	746-778	1.000000	81.4
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	58.2	57.6	0.0	0.0	1.558772	34776	35839	1238-1321	1.000000	71.7
petN-psbM_p2	petN-psbM	2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.6	57.6	0.0	0.0	1.758286	34776	35839	1177-1260	1.000000	70.3
petN-psbM_p3	petN-psbM	3	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACTGCTTTTCTGCT	20	21	50.0	42.9	58.2	57.2	0.0	0.0	1.930302	34776	35839	1217-1300	1.000000	68.8
petN-psbM_p4	petN-psbM	4	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTC	20	20	50.0	45.0	58.2	56.1	0.0	0.0	2.081320	34776	35839	1238-1321	1.000000	67.5
petN-psbM_p5	petN-psbM	5	ATGGGGAAGAAGTGGACTCT	AGTTCCTACTGCTTTTCTGCT	20	21	50.0	42.9	57.6	57.2	0.0	0.0	2.129816	34776	35839	1156-1239	1.000000	67.4
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.8	57.8	0.0	1.1	0.374855	42472	42767	449-456	1.000000	80.0
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	20	20	55.0	50.0	58.3	57.8	0.0	1.1	0.425837	42472	42767	447-454	1.000000	79.6
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	TTGCTTCTCCTGATGGTTGG	TACCATTCGACCATATCCGC	20	20	50.0	50.0	57.8	57.3	0.0	0.0	0.941166	42472	42767	422-429	1.000000	75.7
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	GCTTCTCCTGATGGTTGGTC	TACCATTCGACCATATCCGC	20	20	55.0	50.0	58.3	57.3	0.0	0.0	0.992148	42472	42767	420-427	1.000000	75.3
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	CTCCTGATGGTTGGTCAAGT	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.1	57.8	0.0	1.1	1.036941	42472	42767	443-450	1.000000	74.8
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCATATTCGTAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	22	20	31.8	55.0	53.9	58.1	0.0	0.0	6.230058	57098	58215	929-1185	1.000000	46.6
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	ATCATATTCGTAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	23	20	30.4	55.0	54.5	58.1	0.0	0.0	6.643914	57098	58215	930-1186	1.000000	46.6
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TCATATTCGTAAAGCAGAAACAT	CCGAGAAAGTCTACGGTTCG	23	20	30.4	55.0	54.5	58.1	0.0	0.0	6.643914	57098	58215	929-1185	1.000000	46.6
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TCATATTCGTAAAGCAGAAACA	AGTGCATGTTGGGTCTTTGA	22	20	31.8	45.0	53.9	57.6	0.0	0.0	6.485833	57098	58215	993-1249	1.000000	46.5
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TCATATTCGTAAAGCAGAAACA	GAGCCCAATGCACAAAAAGT	22	20	31.8	45.0	53.9	57.5	0.0	0.0	6.654048	57098	58215	1010-1266	1.000000	46.4
rbcL-accD_p1	rbcL-accD	1	AATGGAGCCCTGAACTAGCT	AAACCGCCCGTTGATTTACT	20	20	50.0	45.0	58.4	58.1	0.0	0.0	0.510340	63382	64008	781-796	1.000000	79.2
rbcL-accD_p2	rbcL-accD	2	AATGGAGCCCTGAACTAGCT	AACCGCCCGTTGATTTACTT	20	20	50.0	45.0	58.4	58.1	0.0	0.0	0.510340	63382	64008	780-795	1.000000	79.2
rbcL-accD_p3	rbcL-accD	3	AAATGGAGCCCTGAACTAGC	AACCGCCCGTTGATTTACTT	20	20	50.0	45.0	57.6	58.1	0.0	0.0	0.526597	63382	64008	781-796	1.000000	79.1
rbcL-accD_p4	rbcL-accD	4	AAATGGAGCCCTGAACTAGC	AAACCGCCCGTTGATTTACT	20	20	50.0	45.0	57.6	58.1	0.0	0.0	0.526597	63382	64008	782-797	1.000000	79.0
rbcL-accD_p5	rbcL-accD	5	AATGGAGCCCTGAACTAGCT	TCAATAGGACCAAAACCGCC	20	20	50.0	50.0	58.4	58.2	0.0	0.0	0.581607	63382	64008	793-808	1.000000	78.6
petB_p1	petB	1	CTAGATCGCGTCCAAAACCA	AACTTATGCGGTGGTTGGAG	20	20	50.0	50.0	58.0	58.2	5.8	0.0	0.183366	81484	82880	2026-2065	1.000000	80.3
petB_p2	petB	2	CTAGATCGCGTCCAAAACCA	TAAGCGAACCAGCCTATCCT	20	20	50.0	50.0	58.0	58.2	5.8	0.0	0.215060	81484	82880	2054-2093	1.000000	80.0
petB_p3	petB	3	AACAGCTCCTTTGATGGGTG	AACTTATGCGGTGGTTGGAG	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.263643	81484	82880	1941-1980	1.000000	79.7
petB_p4	petB	4	AACAGCTCCTTTGATGGGTG	TAAGCGAACCAGCCTATCCT	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.295336	81484	82880	1969-2008	1.000000	79.4
petB_p5	petB	5	CTAGATCGCGTCCAAAACCA	GTTCAAGTCACACACTCCCA	20	20	50.0	50.0	58.0	57.7	5.8	0.0	0.330166	81484	82880	1928-1967	1.000000	79.1
rpl22-rps19_p1	rpl22-rps19	1	GGACCGTCCACGAATTTGAT	AAATTTCCGAGGACACGCAA	20	20	50.0	45.0	58.3	58.4	13.0	0.0	0.665814	90352	90427	217-257	1.000000	83.0
rpl22-rps19_p2	rpl22-rps19	2	GGACCGTCCACGAATTTGAT	AATTTCCGAGGACACGCAAA	20	20	50.0	45.0	58.3	58.4	13.0	0.0	0.665814	90352	90427	216-256	1.000000	83.0
rpl22-rps19_p3	rpl22-rps19	3	GGACCGTCCACGAATTTGAT	ATTTCCGAGGACACGCAAAA	20	20	50.0	45.0	58.3	58.4	13.0	0.0	0.665814	90352	90427	215-255	1.000000	83.0
rpl22-rps19_p4	rpl22-rps19	4	GGACCGTCCACGAATTTGAT	TAAATTTCCGAGGACACGCA	20	20	50.0	45.0	58.3	57.5	13.0	0.0	0.724898	90352	90427	218-258	1.000000	82.5
rpl22-rps19_p5	rpl22-rps19	5	TAGGACCGTCCACGAATTTG	ATTTCCGAGGACACGCAAAA	20	20	50.0	45.0	57.6	58.4	19.9	0.0	0.773517	90352	90427	217-257	1.000000	82.1
ycf1_p1	ycf1	1	CCCGGAACTTCTTCGTAGTG	TGTTCGTTGGATCCCTAGGA	20	20	55.0	50.0	58.0	57.8	0.7	11.5	0.249111	115095	117008	2753-2864	0.750000	80.0
ycf1_p2	ycf1	2	CCGGAACTTCTTCGTAGTGG	TGTTCGTTGGATCCCTAGGA	20	20	55.0	50.0	58.0	57.8	0.0	11.5	0.249111	115095	117008	2752-2863	0.750000	80.0
ycf1_p3	ycf1	3	CCCGGAACTTCTTCGTAGTG	GTGGGCGCATCTCTTCTTAT	20	20	55.0	50.0	58.0	57.8	0.7	10.8	0.254549	115095	117008	2279-2390	0.750000	80.0
ycf1_p4	ycf1	4	CCGGAACTTCTTCGTAGTGG	GTGGGCGCATCTCTTCTTAT	20	20	55.0	50.0	58.0	57.8	0.0	10.8	0.254549	115095	117008	2278-2389	0.750000	80.0
ycf1_p5	ycf1	5	ATCTCCCCAGGTAGGATTCG	TGTTCGTTGGATCCCTAGGA	20	20	55.0	50.0	58.0	57.8	0.0	11.5	0.258185	115095	117008	2909-2996	0.625000	64.1
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGGAAATCTCTTTCTACCGGA	AGCGTGTCTACCGATTTCAC	21	20	47.6	50.0	56.8	58.0	9.5	0.0	2.194456	120453	121323	963-1030	1.000000	68.1
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGGAAATCTCTTTCTACCGGA	CAGCGTGTCTACCGATTTCA	21	20	47.6	50.0	56.8	58.0	9.5	0.0	2.195826	120453	121323	964-1031	1.000000	68.1
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGGGAAATCTCTTTCTACCGG	AGCGTGTCTACCGATTTCAC	21	20	47.6	50.0	56.8	58.0	0.0	0.0	2.202869	120453	121323	964-1031	1.000000	68.0
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGGGAAATCTCTTTCTACCGG	CAGCGTGTCTACCGATTTCA	21	20	47.6	50.0	56.8	58.0	0.0	0.0	2.204239	120453	121323	965-1032	1.000000	68.0
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGCGTTGTCATTAGGGAAATCT	AGCGTGTCTACCGATTTCAC	22	20	40.9	50.0	57.8	58.0	0.0	0.0	2.229954	120453	121323	976-1043	1.000000	67.8
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	GTGAAATCGGTAGACACGCT	ACAATGGAAACGATCGAAAAGG	20	22	50.0	40.9	58.0	57.6	0.0	24.3	2.427353	121404	121505	227-235	1.000000	66.1
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	TGAAATCGGTAGACACGCTG	ACAATGGAAACGATCGAAAAGG	20	22	50.0	40.9	58.0	57.6	0.0	24.3	2.428723	121404	121505	226-234	1.000000	66.1
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	CTCTTAGGAAGCAGTGCGAG	ACAATGGAAACGATCGAAAAGG	20	22	55.0	40.9	58.1	57.6	10.0	24.3	2.499604	121404	121505	206-214	1.000000	66.1
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	CTTAGGAAGCAGTGCGAGAG	ACAATGGAAACGATCGAAAAGG	20	22	55.0	40.9	58.1	57.6	10.0	24.3	2.499604	121404	121505	204-212	1.000000	66.1
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	AACAAGCCGCTATGGTGAAA	ACAATGGAAACGATCGAAAAGG	20	22	45.0	40.9	58.1	57.6	0.0	24.3	2.515235	121404	121505	241-249	1.000000	65.1