primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	matK-rps16	1	CCCTGATCATGAGCAAGTCC	GGTGCTCAACCTACAGGAAC	20	20	55.0	55.0	58.0	58.2	15.6	1.3	0.225764	3672	5311	1754-1856	1.000000	83.1
matK-rps16_p2	matK-rps16	2	CCCTGATCATGAGCAAGTCC	GTGCTCAACCTACAGGAACT	20	20	55.0	50.0	58.0	57.2	15.6	1.3	0.876890	3672	5311	1753-1855	1.000000	77.9
matK-rps16_p3	matK-rps16	3	CCTGATCATGAGCAAGTCCA	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	57.3	58.2	15.6	1.3	0.894780	3672	5311	1753-1855	1.000000	77.8
matK-rps16_p4	matK-rps16	4	ACCCTGATCATGAGCAAGTC	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	57.3	58.2	15.6	1.3	0.896655	3672	5311	1755-1857	1.000000	77.7
matK-rps16_p5	matK-rps16	5	AACCCTGATCATGAGCAAGTC	GGTGCTCAACCTACAGGAAC	21	20	47.6	55.0	58.0	58.2	15.6	1.3	1.193685	3672	5311	1756-1858	1.000000	75.4
rps16-trnQ(UUG)_p1	rps16-trnQ(UUG)	1	CGGATCTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.1	58.1	0.0	16.9	0.161467	6448	8095	1734-1750	1.000000	79.7
rps16-trnQ(UUG)_p2	rps16-trnQ(UUG)	2	ATCTGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	0.0	16.9	0.162155	6448	8095	1731-1747	1.000000	79.7
rps16-trnQ(UUG)_p3	rps16-trnQ(UUG)	3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6448	8095	1720-1736	1.000000	79.3
rps16-trnQ(UUG)_p4	rps16-trnQ(UUG)	4	CCACAACGGATCTGTCCTTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.3	58.1	7.9	16.9	0.349248	6448	8095	1740-1756	1.000000	78.2
rps16-trnQ(UUG)_p5	rps16-trnQ(UUG)	5	GGATCTGTCCTTCAAGTCGC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	0.0	16.9	0.429912	6448	8095	1733-1749	1.000000	77.5
trnS(GCU)-trnG(GCC)_p1	trnS(GCU)-trnG(GCC)	1	CAATCCGACGCTTTAGTCCA	AGACCGAAAGACCCTTTAACT	20	21	50.0	42.9	58.0	56.6	0.0	0.0	2.425660	9417	10115	798-828	1.000000	62.8
trnS(GCU)-trnG(GCC)_p2	trnS(GCU)-trnG(GCC)	2	AATCCGACGCTTTAGTCCAC	AGACCGAAAGACCCTTTAACT	20	21	50.0	42.9	58.0	56.6	0.0	0.0	2.427048	9417	10115	797-827	1.000000	62.8
trnS(GCU)-trnG(GCC)_p3	trnS(GCU)-trnG(GCC)	3	ATCAACGGAACCGGAAAGAG	AGACCGAAAGACCCTTTAACT	20	21	50.0	42.9	57.9	56.6	9.5	0.0	2.515840	9417	10115	862-892	1.000000	61.9
trnS(GCU)-trnG(GCC)_p4	trnS(GCU)-trnG(GCC)	4	CATCAACGGAACCGGAAAGA	AGACCGAAAGACCCTTTAACT	20	21	50.0	42.9	58.2	56.6	9.5	0.0	2.617743	9417	10115	863-893	1.000000	61.1
trnS(GCU)-trnG(GCC)_p5	trnS(GCU)-trnG(GCC)	5	TCATCAACGGAACCGGAAAG	AGACCGAAAGACCCTTTAACT	20	21	50.0	42.9	58.2	56.6	9.5	0.0	2.617743	9417	10115	864-894	1.000000	61.1
atpA-atpF_p1	atpA-atpF	1	ATGCCATCGCCTACTTGAAG	TGTTGGGAGCGATGAAAGAA	20	20	50.0	45.0	58.0	57.4	0.0	0.0	0.600656	12749	12823	207-224	1.000000	82.5
atpA-atpF_p2	atpA-atpF	2	ATGCCATCGCCTACTTGAAG	ATATTGGCATGTTGGGAGCG	20	20	50.0	50.0	58.0	58.7	0.0	0.0	0.719484	12749	12823	216-233	1.000000	81.4
atpA-atpF_p3	atpA-atpF	3	ATGCCATCGCCTACTTGAAG	ATGTTGGGAGCGATGAAAGA	20	20	50.0	45.0	58.0	57.2	0.0	0.0	0.828431	12749	12823	208-225	1.000000	80.7
atpA-atpF_p4	atpA-atpF	4	ATGCCATCGCCTACTTGAAG	CATGTTGGGAGCGATGAAAG	20	20	50.0	50.0	58.0	57.2	0.0	0.0	0.879345	12749	12823	209-226	1.000000	80.3
atpA-atpF_p5	atpA-atpF	5	CAATGCCATCGCCTACTTGA	TGTTGGGAGCGATGAAAGAA	20	20	50.0	45.0	58.3	57.4	0.0	0.0	0.892462	12749	12823	209-226	1.000000	80.2
rps2-rpoC2_p1	rps2-rpoC2	1	AACTCCTGCCTCCATCATCT	TTCGAGGGGGAAATGAGAGA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.357147	17427	17631	375-379	1.000000	79.3
rps2-rpoC2_p2	rps2-rpoC2	2	TGCCTCCATCATCTCTTCCA	TTCGAGGGGGAAATGAGAGA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.364021	17427	17631	369-373	1.000000	79.2
rps2-rpoC2_p3	rps2-rpoC2	3	TCCTGCCTCCATCATCTCTT	TTCGAGGGGGAAATGAGAGA	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.434938	17427	17631	372-376	1.000000	78.7
rps2-rpoC2_p4	rps2-rpoC2	4	GAACTCCTGCCTCCATCATC	TTCGAGGGGGAAATGAGAGA	20	20	55.0	50.0	57.7	57.8	0.0	0.0	0.500676	17427	17631	376-380	1.000000	78.1
rps2-rpoC2_p5	rps2-rpoC2	5	CTCCTGCCTCCATCATCTCT	TTCGAGGGGGAAATGAGAGA	20	20	55.0	50.0	58.3	57.8	0.0	0.0	0.528170	17427	17631	373-377	1.000000	77.9
psaA-ycf3_p1	psaA-ycf3	1	CTTCTGGTTCCGGCGAAC	GGAAACAGGCTATAGCGCTT	18	20	61.1	50.0	58.4	58.0	9.3	18.7	2.482438	43581	44318	828-856	1.000000	63.4
psaA-ycf3_p2	psaA-ycf3	2	CTTCTGGTTCCGGCGAAC	ATTGGTTGAAGATCACGGGG	18	20	61.1	50.0	58.4	57.9	9.3	0.0	2.566136	43581	44318	780-808	1.000000	62.9
psaA-ycf3_p3	psaA-ycf3	3	CTTCTGGTTCCGGCGAAC	TGGTTTGATCAAGCAGCTGA	18	20	61.1	45.0	58.4	57.7	9.3	20.4	2.712499	43581	44318	853-881	1.000000	61.4
psaA-ycf3_p4	psaA-ycf3	4	CTTCTGGTTCCGGCGAAC	AGGCTATAGCGCTTACTCCT	18	20	61.1	50.0	58.4	57.7	9.3	18.7	2.733438	43581	44318	822-850	1.000000	61.4
psaA-ycf3_p5	psaA-ycf3	5	CTTCTGGTTCCGGCGAAC	TTGGAAACAGGCTATAGCGC	18	20	61.1	50.0	58.4	58.3	9.3	18.3	2.773671	43581	44318	830-858	1.000000	61.0
ndhC-trnM(CAU)_p1	ndhC-trnM(CAU)	1	GGGGCTAAAATCCCGGAAAT	CCCGCCGTATGAAAGCAATA	20	20	50.0	50.0	57.9	58.4	0.7	0.0	0.493466	52394	54328	1934-2092	1.000000	80.6
ndhC-trnM(CAU)_p2	ndhC-trnM(CAU)	2	GGGGCTAAAATCCCGGAAAT	AGGTACCATTGACTCCGGTA	20	20	50.0	50.0	57.9	57.5	0.7	15.9	0.619773	52394	54328	1995-2153	1.000000	79.5
ndhC-trnM(CAU)_p3	ndhC-trnM(CAU)	3	GGGGCTAAAATCCCGGAAAT	TAGGTACCATTGACTCCGGT	20	20	50.0	50.0	57.9	57.5	0.7	15.9	0.619773	52394	54328	1996-2154	1.000000	79.5
ndhC-trnM(CAU)_p4	ndhC-trnM(CAU)	4	GGGGCTAAAATCCCGGAAAT	CCGCCGTATGAAAGCAATAC	20	20	50.0	50.0	57.9	57.3	0.7	0.0	0.745419	52394	54328	1933-2091	1.000000	78.5
ndhC-trnM(CAU)_p5	ndhC-trnM(CAU)	5	TTCTCCGGCCCTTTCCTAAT	CCCGCCGTATGAAAGCAATA	20	20	50.0	50.0	58.4	58.4	0.0	0.0	0.819557	52394	54328	1955-2113	1.000000	77.9
atpB-rbcL_p1	atpB-rbcL	1	ATACGGCCCAGGTTTTGTTT	AACACCCGCTTTGAATCCAA	20	20	45.0	45.0	58.0	58.3	0.0	0.0	0.305431	56502	57293	911-916	1.000000	79.1
atpB-rbcL_p2	atpB-rbcL	2	GGAAACCCCAGAACCAGAAG	AACACCCGCTTTGAATCCAA	20	20	55.0	45.0	58.1	58.3	0.0	0.0	0.390437	56502	57293	882-887	1.000000	78.5
atpB-rbcL_p3	atpB-rbcL	3	GGCGGAAAGGCTACATCTAG	AACACCCGCTTTGAATCCAA	20	20	55.0	45.0	57.8	58.3	0.0	0.0	0.457837	56502	57293	953-958	1.000000	77.9
atpB-rbcL_p4	atpB-rbcL	4	CGGACCGATTATTTGGACGA	AACACCCGCTTTGAATCCAA	20	20	50.0	45.0	57.8	58.3	8.6	0.0	0.514363	56502	57293	930-935	1.000000	77.5
atpB-rbcL_p5	atpB-rbcL	5	TACGGCCCAGGTTTTGTTTT	AACACCCGCTTTGAATCCAA	20	20	45.0	45.0	58.2	58.3	0.0	0.0	0.523554	56502	57293	910-915	1.000000	77.4
ycf4-cemA_p1	ycf4-cemA	1	CATTCCCTTGACCCGTACTG	GAGAAATCCACCAGGGCAAA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.100820	62684	63555	1043-1051	1.000000	80.3
ycf4-cemA_p2	ycf4-cemA	2	ATTCCCTTGACCCGTACTGA	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.129660	62684	63555	1042-1050	1.000000	80.1
ycf4-cemA_p3	ycf4-cemA	3	TTCCCTTGACCCGTACTGAT	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.129660	62684	63555	1041-1049	1.000000	80.1
ycf4-cemA_p4	ycf4-cemA	4	CTTGCGCGTACCAATTGAAG	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.1	58.1	8.4	0.0	0.194446	62684	63555	968-976	1.000000	79.6
ycf4-cemA_p5	ycf4-cemA	5	GAACAAAAAGCCGCCGAATT	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	58.2	58.1	0.0	0.0	0.298310	62684	63555	997-1005	1.000000	78.8
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.9	57.1	6.4	0.0	0.992787	67165	68346	1372-1375	1.000000	72.9
psbE-petL_p2	psbE-petL	2	ACTGACGAATAACCAACCCG	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.9	57.1	0.0	0.0	1.030402	67165	68346	1385-1388	1.000000	72.6
psbE-petL_p3	psbE-petL	3	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	24.9	0.0	1.097796	67165	68346	1295-1298	1.000000	72.1
psbE-petL_p4	psbE-petL	4	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	20	20	45.0	50.0	58.2	57.1	0.0	0.0	1.112474	67165	68346	1381-1384	1.000000	72.0
psbE-petL_p5	psbE-petL	5	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	0.0	0.0	1.128804	67165	68346	1380-1383	1.000000	71.8
ndhF_p1	ndhF	1	GAGATCCGCCGAAGAAGATC	GAGGTAAGATCCGGTGAATCG	20	21	55.0	52.4	57.9	58.0	0.0	0.0	1.073517	112229	114451	2492-2498	1.000000	72.8
ndhF_p2	ndhF	2	ACAGCTTCCGAAATGAAGGG	GAGGTAAGATCCGGTGAATCG	20	21	50.0	52.4	58.2	58.0	3.3	0.0	1.188219	112229	114451	2530-2536	1.000000	71.8
ndhF_p3	ndhF	3	GCAGAATACCGTCACCCATT	GAGGTAAGATCCGGTGAATCG	20	21	50.0	52.4	58.0	58.0	0.0	0.0	1.052991	112229	114451	2628-2634	0.900000	68.8
ndhF_p4	ndhF	4	CAGGAACAAGAGAGATCCGC	GAGGTAAGATCCGGTGAATCG	20	21	55.0	52.4	58.1	58.0	0.0	0.0	1.081357	112229	114451	2503-2509	0.900000	68.6
ndhF_p5	ndhF	5	GCAGAATACCGTCACCCATT	AGGTAAGATCCGGTGAATCG	20	20	50.0	50.0	58.0	56.8	0.0	0.0	1.218364	112229	114451	2627-2633	0.900000	67.4
ndhF-rpl32_p1	ndhF-rpl32	1	GTTGCCGTCGGAAAAAGAAG	TTTAACGCTGCCCCATATCC	20	20	50.0	50.0	58.0	58.0	17.5	0.0	0.056754	114452	114930	661-666	1.000000	82.1
ndhF-rpl32_p2	ndhF-rpl32	2	TGCCGTCGGAAAAAGAAGAA	TTTAACGCTGCCCCATATCC	20	20	45.0	50.0	58.0	58.0	17.5	0.0	0.068044	114452	114930	659-664	1.000000	82.0
ndhF-rpl32_p3	ndhF-rpl32	3	TTGCCGTCGGAAAAAGAAGA	TTTAACGCTGCCCCATATCC	20	20	45.0	50.0	58.0	58.0	17.5	0.0	0.068044	114452	114930	660-665	1.000000	82.0
ndhF-rpl32_p4	ndhF-rpl32	4	GTTGCCGTCGGAAAAAGAAG	AAATGCTTTTAACGCTGCCC	20	20	50.0	45.0	58.0	57.9	17.5	0.0	0.111263	114452	114930	672-673	0.900000	76.5
ndhF-rpl32_p5	ndhF-rpl32	5	TGCCGTCGGAAAAAGAAGAA	AAATGCTTTTAACGCTGCCC	20	20	45.0	45.0	58.0	57.9	17.5	0.0	0.122552	114452	114930	670-671	0.900000	76.5
rpl32-trnL(UAG)_p1	rpl32-trnL(UAG)	1	GGATATGGGGCAGCGTTAAA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.148618	115093	115634	669-675	1.000000	81.3
rpl32-trnL(UAG)_p2	rpl32-trnL(UAG)	2	GGGCAGCGTTAAAAGCATTT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	57.9	57.9	6.9	0.0	0.203126	115093	115634	662-668	1.000000	80.9
rpl32-trnL(UAG)_p3	rpl32-trnL(UAG)	3	GGATATGGGGCAGCGTTAAA	AGCAGCGTGTCTACCAATTT	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.209344	115093	115634	662-668	1.000000	80.8
rpl32-trnL(UAG)_p4	rpl32-trnL(UAG)	4	GGATATGGGGCAGCGTTAAA	CCTAAGAGCAGCGTGTCTAC	20	20	50.0	55.0	58.0	57.8	0.0	0.0	0.220635	115093	115634	668-674	1.000000	80.7
rpl32-trnL(UAG)_p5	rpl32-trnL(UAG)	5	GGATATGGGGCAGCGTTAAA	CTAAGAGCAGCGTGTCTACC	20	20	50.0	55.0	58.0	57.8	0.0	0.0	0.220635	115093	115634	667-673	1.000000	80.7
ndhA_p1	ndhA	1	TGAACTCAGTTACCATAGGAAACA	CCCACCGGGTTTTATCAACT	24	20	37.5	50.0	57.6	57.8	0.0	6.3	4.637773	120990	123171	2395-2413	1.000000	43.8
ndhA_p2	ndhA	2	TCATGAACTCAGTTACCATAGGA	CCCACCGGGTTTTATCAACT	23	20	39.1	50.0	56.5	57.8	0.0	6.3	4.746193	120990	123171	2398-2416	1.000000	42.9
ndhA_p3	ndhA	3	TGTTTGAGACAAGTTGTGAA	CCCACCGGGTTTTATCAACT	20	20	35.0	50.0	53.2	57.8	14.6	6.3	5.009506	120990	123171	2327-2345	1.000000	40.9
ndhA_p4	ndhA	4	TTGTTTGAGACAAGTTGTGA	CCCACCGGGTTTTATCAACT	20	20	35.0	50.0	53.2	57.8	14.6	6.3	5.009506	120990	123171	2328-2346	1.000000	40.9
ndhA_p5	ndhA	5	TCATGAACTCAGTTACCATAGGAA	CCCACCGGGTTTTATCAACT	24	20	37.5	50.0	57.1	57.8	0.0	6.3	5.077209	120990	123171	2398-2416	1.000000	40.9
ycf1_p1	ycf1	1	TCAGGAAGTTGCAATAGTAGCA	GAGGGGGTCAGCATTCAAAA	22	20	40.9	50.0	57.4	58.1	0.6	0.0	2.628346	128237	128356	246-270	1.000000	64.0
ycf1_p2	ycf1	2	TCAGGAAGTTGCAATAGTAGCA	AGAAATTGGGTAGGGAGGGG	22	20	40.9	55.0	57.4	58.4	0.6	0.0	2.943277	128237	128356	260-284	1.000000	61.2
ycf1_p3	ycf1	3	CAGGAAGTTGCAATAGTAGCA	GAGGGGGTCAGCATTCAAAA	21	20	42.9	50.0	56.0	58.1	0.6	0.0	3.106582	128237	128356	245-269	1.000000	60.2
ycf1_p4	ycf1	4	TTCAGGAAGTTGCAATAGTAGCA	GAGGGGGTCAGCATTCAAAA	23	20	39.1	50.0	58.1	58.1	0.6	0.0	3.171215	128237	128356	247-271	1.000000	59.6
ycf1_p5	ycf1	5	TCAGGAAGTTGCAATAGTAGCAT	GAGGGGGTCAGCATTCAAAA	23	20	39.1	50.0	57.9	58.1	0.6	0.0	3.179597	128237	128356	246-270	1.000000	59.6