primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl22_p1	rps19-rpl22	1	TCGAGGACATGCAAAAAGTGA	TGTTTGGCTAAAGCTTTGGC	21	20	42.9	45.0	58.2	57.5	0.0	32.6	1.679622	2425	2441	104-104	1.000000	68.1
rps19-rpl22_p2	rps19-rpl22	2	GGAGAATTTGCACCTACACG	TGTTTGGCTAAAGCTTTGGC	20	20	50.0	45.0	56.8	57.5	0.0	32.6	1.708157	2425	2441	130-130	1.000000	67.6
rps19-rpl22_p3	rps19-rpl22	3	AGGAGAATTTGCACCTACACG	TGTTTGGCTAAAGCTTTGGC	21	20	47.6	45.0	58.3	57.5	0.0	32.6	1.824614	2425	2441	131-131	1.000000	66.6
rps19-rpl22_p4	rps19-rpl22	4	GGAGAATTTGCACCTACACGA	TGTTTGGCTAAAGCTTTGGC	21	20	47.6	45.0	58.3	57.5	0.0	32.6	1.829210	2425	2441	130-130	1.000000	66.6
rps19-rpl22_p5	rps19-rpl22	5	CGAGGACATGCAAAAAGTGA	TGTTTGGCTAAAGCTTTGGC	20	20	45.0	45.0	56.7	57.5	0.0	32.6	1.873511	2425	2441	103-103	1.000000	66.6
psbN-psbT_p1	psbN-psbT	1	ACAGCTTTTGGACAACCTTCT	TCGTGAAACACCTAAAATTCAAACT	21	25	42.9	32.0	58.0	57.9	0.0	0.0	6.109375	10298	10384	187-187	1.000000	44.3
psbN-psbT_p2	psbN-psbT	2	ACAGCTTTTGGACAACCTTCT	TCGTGAAACACCTAAAATTCA	21	21	42.9	33.3	58.0	53.7	0.0	0.0	6.329135	10298	10384	187-187	1.000000	44.3
psbN-psbT_p3	psbN-psbT	3	ACAGCTTTTGGACAACCTTCT	TCGTGAAACACCTAAAATTCAAAC	21	24	42.9	33.3	58.0	56.6	0.0	0.0	6.376033	10298	10384	187-187	1.000000	44.3
psbN-psbT_p4	psbN-psbT	4	ACAGCTTTTGGACAACCTTCT	CGTGAAACACCTAAAATTCAAACT	21	24	42.9	33.3	58.0	56.6	0.0	0.0	6.383612	10298	10384	186-186	1.000000	44.3
psbN-psbT_p5	psbN-psbT	5	ACAGCTTTTGGACAACCTTCT	TCGTGAAACACCTAAAATTCAA	21	22	42.9	31.8	58.0	54.5	0.0	0.0	6.491073	10298	10384	187-187	1.000000	44.3
trnL-UAA_p1	trnL-UAA	1	TGGATTTGGAAAAGTGCGGA	GAGTCGAACCGATGACCATC	20	20	45.0	55.0	58.0	58.2	0.0	0.0	0.170257	32342	32725	1207-1207	1.000000	86.1
trnL-UAA_p2	trnL-UAA	2	ACTTGGATTGGTTGGCCTTT	GAGTCGAACCGATGACCATC	20	20	45.0	55.0	57.9	58.2	3.3	0.0	0.260190	32342	32725	1105-1105	1.000000	86.0
trnL-UAA_p3	trnL-UAA	3	TGGATTTGGAAAAGTGCGGA	TCTGACCTCTGAGCTAAGCA	20	20	45.0	50.0	58.0	57.8	0.0	0.0	0.233934	32342	32725	1169-1169	1.000000	85.9
trnL-UAA_p4	trnL-UAA	4	ACTTGGATTGGTTGGCCTTT	TCTGACCTCTGAGCTAAGCA	20	20	45.0	50.0	57.9	57.8	3.3	0.0	0.323867	32342	32725	1067-1067	1.000000	85.7
trnL-UAA_p5	trnL-UAA	5	TCTACCAACTGAGCTACCCC	GAGTCGAACCGATGACCATC	20	20	55.0	55.0	58.1	58.2	0.0	0.0	0.296569	32342	32725	780-780	0.500000	57.6
trnfM-CAU-trnG-GCC_p1	trnfM-CAU-trnG-GCC	1	GTAGCTCGCAAGGCTCATAA	AATATGCGGGTTCGATTCCC	20	20	50.0	50.0	57.8	58.1	0.0	14.1	0.332859	41550	41662	196-198	1.000000	81.8
trnfM-CAU-trnG-GCC_p2	trnfM-CAU-trnG-GCC	2	TAGCTCGCAAGGCTCATAAC	AATATGCGGGTTCGATTCCC	20	20	50.0	50.0	57.8	58.1	0.0	14.1	0.332859	41550	41662	195-197	1.000000	81.8
trnfM-CAU-trnG-GCC_p3	trnfM-CAU-trnG-GCC	3	GTAGCTCGCAAGGCTCATAA	AAGGAGAATATGCGGGTTCG	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.465562	41550	41662	202-204	1.000000	80.6
trnfM-CAU-trnG-GCC_p4	trnfM-CAU-trnG-GCC	4	TAGCTCGCAAGGCTCATAAC	AAGGAGAATATGCGGGTTCG	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.465562	41550	41662	201-203	1.000000	80.6
trnfM-CAU-trnG-GCC_p5	trnfM-CAU-trnG-GCC	5	TAGCTCGCAAGGCTCATAAC	TTGCCAAGGAGAATATGCGG	20	20	50.0	50.0	57.8	58.3	0.0	0.0	0.545929	41550	41662	206-208	1.000000	79.9
psbD-trnT-GGU_p1	psbD-trnT-GGU	1	GCCAGTCATCCATGCTATCA	GTGGTAGAGTAACGCCATGG	20	20	50.0	55.0	57.4	58.1	0.0	7.6	0.620211	44997	45509	637-638	1.000000	77.5
psbD-trnT-GGU_p2	psbD-trnT-GGU	2	GCCAGTCATCCATGCTATCA	TCAGTGGTAGAGTAACGCCA	20	20	50.0	50.0	57.4	58.1	0.0	0.0	0.642366	44997	45509	640-641	1.000000	77.4
psbD-trnT-GGU_p3	psbD-trnT-GGU	3	TCTTAGCCAGTCATCCATGC	GTGGTAGAGTAACGCCATGG	20	20	50.0	55.0	57.4	58.1	0.0	7.6	0.691582	44997	45509	642-643	1.000000	77.0
psbD-trnT-GGU_p4	psbD-trnT-GGU	4	CTTAGCCAGTCATCCATGCT	GTGGTAGAGTAACGCCATGG	20	20	50.0	55.0	57.4	58.1	0.0	7.6	0.698883	44997	45509	641-642	1.000000	76.9
psbD-trnT-GGU_p5	psbD-trnT-GGU	5	TCTTAGCCAGTCATCCATGC	TCAGTGGTAGAGTAACGCCA	20	20	50.0	50.0	57.4	58.1	0.0	0.0	0.713737	44997	45509	645-646	1.000000	76.8
chlL_p1	chlL	1	CGCAATCAAGCAGGAAAAGG	TAGTTTTGGTGCCATGCCTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.021545	95102	95977	1627-1627	0.500000	59.8
chlL_p2	chlL	2	AGTGGGAAGTCGGGAAGTTA	TAGTTTTGGTGCCATGCCTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.028050	95102	95977	1505-1505	0.500000	59.8
chlL_p3	chlL	3	TAGTGGGAAGTCGGGAAGTT	TAGTTTTGGTGCCATGCCTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.028050	95102	95977	1506-1506	0.500000	59.8
chlL_p4	chlL	4	CGCAATCAAGCAGGAAAAGG	CTGGACAACGATGTGCCATA	20	20	50.0	50.0	58.0	58.0	0.0	14.5	0.036067	95102	95977	1736-1736	0.500000	59.7
chlL_p5	chlL	5	CGCAATCAAGCAGGAAAAGG	TGGACAACGATGTGCCATAG	20	20	50.0	50.0	58.0	58.0	0.0	14.5	0.036067	95102	95977	1735-1735	0.500000	59.7
chlN_p1	chlN	1	GTTGCGGAGGCTATGTTGTA	AGCACACAAAGCTGTAAGGG	20	20	50.0	50.0	58.0	58.4	0.0	0.0	0.409334	96017	97426	2276-2276	0.500000	56.7
chlN_p2	chlN	2	GTTGCGGAGGCTATGTTGTA	GCACACAAAGCTGTAAGGGA	20	20	50.0	50.0	58.0	58.4	0.0	0.0	0.413938	96017	97426	2275-2275	0.500000	56.7
chlN_p3	chlN	3	GTTGCGGAGGCTATGTTGTA	CCCATAGCACACAAAGCTGT	20	20	50.0	50.0	58.0	58.5	0.0	0.0	0.480494	96017	97426	2281-2281	0.500000	56.2
chlN_p4	chlN	4	TGCTGCTTCAGTTCGAGAAA	AGCACACAAAGCTGTAAGGG	20	20	45.0	50.0	57.8	58.4	0.0	0.0	0.629734	96017	97426	2080-2080	0.500000	55.0
chlN_p5	chlN	5	TTGCTGCTTCAGTTCGAGAA	AGCACACAAAGCTGTAAGGG	20	20	45.0	50.0	57.8	58.4	0.0	0.0	0.629734	96017	97426	2081-2081	0.500000	55.0
ycf1_p1	ycf1	1	ACCAATGCACCTTTTGGAGA	ACGAATAGTTTGATGGCGCT	20	20	45.0	45.0	57.6	58.0	20.9	7.6	0.404240	100933	101052	1503-1503	0.500000	56.8
ycf1_p2	ycf1	2	AGGCCTCTTTTTGGAAACCA	ACGAATAGTTTGATGGCGCT	20	20	45.0	45.0	57.5	58.0	0.0	7.6	0.494232	100933	101052	1472-1472	0.500000	56.0
ycf1_p3	ycf1	3	ACCAATGCACCTTTTGGAGA	TTTGATGGCGCTCTCTTTCA	20	20	45.0	45.0	57.6	57.8	20.9	7.6	0.563542	100933	101052	1495-1495	0.500000	55.5
ycf1_p4	ycf1	4	ACCAATGCACCTTTTGGAGA	AGTTTGATGGCGCTCTCTTT	20	20	45.0	45.0	57.6	57.8	20.9	7.6	0.571192	100933	101052	1497-1497	0.500000	55.4
ycf1_p5	ycf1	5	AGGCCTCTTTTTGGAAACCA	TTTGATGGCGCTCTCTTTCA	20	20	45.0	45.0	57.5	57.8	0.0	7.6	0.653535	100933	101052	1464-1464	0.500000	54.8
ndhH_p1	ndhH	1	TTCTTTGGCCTAGTTTTCGA	TGCCATAAGAAGTCCAAGCG	20	20	40.0	50.0	54.9	58.3	3.3	0.0	3.359191	102932	104107	2219-2219	0.500000	33.1
ndhH_p2	ndhH	2	TTTCTTTGGCCTAGTTTTCGA	TGCCATAAGAAGTCCAAGCG	21	20	38.1	50.0	55.7	58.3	3.3	0.0	3.535236	102932	104107	2220-2220	0.500000	31.7
ndhH_p3	ndhH	3	TCTTTGGCCTAGTTTTCGAT	TGCCATAAGAAGTCCAAGCG	20	20	40.0	50.0	54.7	58.3	3.3	0.0	3.597552	102932	104107	2218-2218	0.500000	31.2
ndhH_p4	ndhH	4	CGATTAGAAGATTTGGCATGTGT	TGCCATAAGAAGTCCAAGCG	23	20	39.1	50.0	57.5	58.3	0.0	0.0	3.752301	102932	104107	2202-2202	0.500000	30.0
ndhH_p5	ndhH	5	TCTTTGGCCTAGTTTTCGATT	TGCCATAAGAAGTCCAAGCG	21	20	38.1	50.0	55.5	58.3	3.3	0.0	3.759221	102932	104107	2218-2218	0.500000	29.9
ndhA_p1	ndhA	1	ATCCGGACCAATGTTACGTG	GACCAATATATCGTGCCGCT	20	20	50.0	50.0	58.0	57.9	6.1	0.0	0.098828	104109	105939	2508-2508	0.500000	59.2
ndhA_p2	ndhA	2	ATCCGGACCAATGTTACGTG	AACACATACTTCGCAGGCAA	20	20	50.0	45.0	58.0	58.1	6.1	0.0	0.127666	104109	105939	2651-2651	0.500000	59.0
ndhA_p3	ndhA	3	ATCCGGACCAATGTTACGTG	ACACATACTTCGCAGGCAAT	20	20	50.0	45.0	58.0	57.9	6.1	0.0	0.132402	104109	105939	2650-2650	0.500000	58.9
ndhA_p4	ndhA	4	GGCTTATCCGGACCAATGTT	GACCAATATATCGTGCCGCT	20	20	50.0	50.0	57.9	57.9	6.1	0.0	0.133068	104109	105939	2513-2513	0.500000	58.9
ndhA_p5	ndhA	5	AAATTGGGGCTTATCCGGAC	GACCAATATATCGTGCCGCT	20	20	50.0	50.0	57.9	57.9	6.1	0.0	0.147651	104109	105939	2520-2520	0.500000	58.8
ndhI_p1	ndhI	1	GGGGATGGAATTTATGGCGT	AGCGCCTATTAATGCAACCA	20	20	50.0	45.0	58.0	57.9	0.0	2.3	0.055958	106020	106571	1746-1746	0.500000	59.6
ndhI_p2	ndhI	2	TGGGGATGGAATTTATGGCG	AGCGCCTATTAATGCAACCA	20	20	50.0	45.0	58.0	57.9	0.0	2.3	0.057378	106020	106571	1747-1747	0.500000	59.5
ndhI_p3	ndhI	3	GGGGATGGAATTTATGGCGT	AAAATTTGTGCGGCAGCTAC	20	20	50.0	45.0	58.0	57.9	0.0	10.9	0.069197	106020	106571	1427-1427	0.500000	59.4
ndhI_p4	ndhI	4	TGGGGATGGAATTTATGGCG	AAAATTTGTGCGGCAGCTAC	20	20	50.0	45.0	58.0	57.9	0.0	10.9	0.070617	106020	106571	1428-1428	0.500000	59.4
ndhI_p5	ndhI	5	GGGGATGGAATTTATGGCGT	ATTTTCTCGGCGGGCTAAAA	20	20	50.0	45.0	58.0	58.2	0.0	7.2	0.175645	106020	106571	1770-1770	0.500000	58.6
ndhG_p1	ndhG	1	TTGCCTGCGAAGTATGTGTT	ACATCCATCCCAAGGTACCA	20	20	45.0	50.0	58.1	58.0	0.0	15.9	0.129314	106703	107278	1649-1649	0.500000	59.0
ndhG_p2	ndhG	2	ATTGCCTGCGAAGTATGTGT	ACATCCATCCCAAGGTACCA	20	20	45.0	50.0	57.9	58.0	0.0	15.9	0.134051	106703	107278	1650-1650	0.500000	58.9
ndhG_p3	ndhG	3	TTGCCTGCGAAGTATGTGTT	TCCAATAGCAGCTTCAGCAG	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.143228	106703	107278	1323-1323	0.500000	58.9
ndhG_p4	ndhG	4	ATTGCCTGCGAAGTATGTGT	TCCAATAGCAGCTTCAGCAG	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.147965	106703	107278	1324-1324	0.500000	58.8
ndhG_p5	ndhG	5	TGCGAAGTATGTGTTCGTGT	ACATCCATCCCAAGGTACCA	20	20	45.0	50.0	57.9	58.0	7.0	15.9	0.163198	106703	107278	1644-1644	0.500000	58.7
ndhE_p1	ndhE	1	TGGTTGCATTAATAGGCGCT	ACATCCATCCCAAGGTACCA	20	20	45.0	50.0	57.9	58.0	7.6	15.9	0.071525	107308	107610	687-687	0.500000	59.4
ndhE_p2	ndhE	2	GTAGCTGCCGCACAAATTTT	ACATCCATCCCAAGGTACCA	20	20	45.0	50.0	57.9	58.0	2.0	15.9	0.084764	107308	107610	1006-1006	0.500000	59.3
ndhE_p3	ndhE	3	TGGTTGCATTAATAGGCGCT	TTCGCATCGTTTACAACCCA	20	20	45.0	45.0	57.9	58.1	7.6	0.0	0.171063	107308	107610	750-750	0.500000	58.6
ndhE_p4	ndhE	4	GTAGCTGCCGCACAAATTTT	TTCGCATCGTTTACAACCCA	20	20	45.0	45.0	57.9	58.1	2.0	0.0	0.184302	107308	107610	1069-1069	0.500000	58.5
ndhE_p5	ndhE	5	AGCCCGCCGAGAAAATTTAA	ACATCCATCCCAAGGTACCA	20	20	45.0	50.0	58.2	58.0	0.0	15.9	0.191211	107308	107610	659-659	0.500000	58.5
ndhD_p1	ndhD	1	TGGTACCTTGGGATGGATGT	GAACCCACGATCTCCATCAC	20	20	50.0	55.0	58.0	58.1	14.4	0.0	0.078866	108094	109593	2262-2262	0.500000	59.4
ndhD_p2	ndhD	2	TGGTACCTTGGGATGGATGT	ATAAGCCGCCATGGTGAAAT	20	20	50.0	45.0	58.0	57.9	14.4	27.1	0.091968	108094	109593	2095-2095	0.500000	59.3
ndhD_p3	ndhD	3	CTGCTGAAGCTGCTATTGGA	GAACCCACGATCTCCATCAC	20	20	50.0	55.0	58.0	58.1	0.9	0.0	0.092779	108094	109593	2588-2588	0.500000	59.3
ndhD_p4	ndhD	4	CTGCTGAAGCTGCTATTGGA	ATAAGCCGCCATGGTGAAAT	20	20	50.0	45.0	58.0	57.9	0.9	27.1	0.105882	108094	109593	2421-2421	0.500000	59.2
ndhD_p5	ndhD	5	TGGTACCTTGGGATGGATGT	CTAACGCATCTCGGTTCGAA	20	20	50.0	50.0	58.0	58.1	14.4	0.0	0.116047	108094	109593	2045-2045	0.500000	59.1
rpl21_p1	rpl21	1	GTGATGGAGATCGTGGGTTC	ACAAGAACTCCTACCGTAGT	20	20	55.0	45.0	58.1	55.2	0.0	4.9	2.831948	110642	110992	1637-1637	0.500000	37.3
rpl21_p2	rpl21	2	ATTTCACCATGGCGGCTTAT	ACAAGAACTCCTACCGTAGT	20	20	45.0	45.0	57.9	55.2	13.0	4.9	2.845050	110642	110992	1804-1804	0.500000	37.2
rpl21_p3	rpl21	3	ATAGCGTAGTTTGGCAACGT	ACAAGAACTCCTACCGTAGT	20	20	45.0	45.0	57.9	55.2	10.7	4.9	2.862222	110642	110992	1668-1668	0.500000	37.1
rpl21_p4	rpl21	4	TCCTAAGAGCAGCGTGTCTA	ACAAGAACTCCTACCGTAGT	20	20	50.0	45.0	57.9	55.2	0.0	4.9	2.897550	110642	110992	1827-1827	0.500000	36.8
rpl21_p5	rpl21	5	GGGGTATAGCGTAGTTTGGC	ACAAGAACTCCTACCGTAGT	20	20	55.0	45.0	58.1	55.2	0.0	4.9	2.898264	110642	110992	1673-1673	0.500000	36.8
rpl21-ndhF_p1	rpl21-ndhF	1	CTGTTCACCTCCGGTTTCAA	GGAAACTGAGCCGATTTTGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.055594	110993	111400	1175-1175	0.500000	59.6
rpl21-ndhF_p2	rpl21-ndhF	2	CTGTTCACCTCCGGTTTCAA	GAGGAACAAAAGAAGCGCAC	20	20	50.0	50.0	58.0	58.0	0.0	11.5	0.076147	110993	111400	1146-1146	0.500000	59.4
rpl21-ndhF_p3	rpl21-ndhF	3	ACCTCCGGTTTCAATGATGG	GGAAACTGAGCCGATTTTGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.146158	110993	111400	1169-1169	0.500000	58.8
rpl21-ndhF_p4	rpl21-ndhF	4	TCCGGTTTCAATGATGGCAT	GGAAACTGAGCCGATTTTGC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.159744	110993	111400	1166-1166	0.500000	58.7
rpl21-ndhF_p5	rpl21-ndhF	5	ACCTCCGGTTTCAATGATGG	GAGGAACAAAAGAAGCGCAC	20	20	50.0	50.0	57.9	58.0	0.0	11.5	0.166711	110993	111400	1140-1140	0.500000	58.7
ndhF_p1	ndhF	1	CTGTTCACCTCCGGTTTCAA	CGCAATCAAGCAGGAAAAGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.051509	111401	113509	2789-2789	0.500000	59.6
ndhF_p2	ndhF	2	CTGTTCACCTCCGGTTTCAA	AGTGGGAAGTCGGGAAGTTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.058014	111401	113509	2667-2667	0.500000	59.5
ndhF_p3	ndhF	3	CTGTTCACCTCCGGTTTCAA	TAGTGGGAAGTCGGGAAGTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.058014	111401	113509	2668-2668	0.500000	59.5
ndhF_p4	ndhF	4	CTGTTCACCTCCGGTTTCAA	AAGTCGGGAAGTTAGGGGAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.072643	111401	113509	2661-2661	0.500000	59.4
ndhF_p5	ndhF	5	CTGTTCACCTCCGGTTTCAA	CGCGATCGTACCGTCATTAT	20	20	50.0	50.0	58.0	58.0	0.0	17.8	0.078460	111401	113509	2633-2633	0.500000	59.4