primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ycf1	1	TAGCAGAGACCTGGGATAGC	CAATGGGGAATTTGCCGAAC	20	20	55.0	50.0	58.0	58.0	0.0	0.4	0.011257	110147	110266	758-758	0.500000	59.9
ycf1_p2	ycf1	2	ACGGATACTAAGATGCCCGA	CAATGGGGAATTTGCCGAAC	20	20	50.0	50.0	58.0	58.0	0.0	0.4	0.017631	110147	110266	1101-1101	0.500000	59.9
ycf1_p3	ycf1	3	TAGCAGAGACCTGGGATAGC	AATGGGGAATTTGCCGAACT	20	20	55.0	45.0	58.0	58.1	0.0	0.4	0.065314	110147	110266	757-757	0.500000	59.5
ycf1_p4	ycf1	4	TAGCAGAGACCTGGGATAGC	ATGGGGAATTTGCCGAACTT	20	20	55.0	45.0	58.0	58.1	0.0	0.4	0.065314	110147	110266	756-756	0.500000	59.5
ycf1_p5	ycf1	5	ACGGATACTAAGATGCCCGA	ATGGGGAATTTGCCGAACTT	20	20	50.0	45.0	58.0	58.1	0.0	0.4	0.071688	110147	110266	1099-1099	0.500000	59.4
ycf1-rps15_p1	ycf1-rps15	1	AGCCGAGTTTCACATGACAA	CCTGAGTCAGTCTTCCATGC	20	20	45.0	55.0	57.7	58.0	0.0	18.7	0.275901	113275	113700	1000-1000	0.500000	57.8
ycf1-rps15_p2	ycf1-rps15	2	AGCCGAGTTTCACATGACAA	AGTCGTTGACGTTTTCCCAA	20	20	45.0	45.0	57.7	58.0	0.0	3.2	0.281549	113275	113700	809-809	0.500000	57.7
ycf1-rps15_p3	ycf1-rps15	3	AGCCGAGTTTCACATGACAA	AACACCATGCATTGATGGGT	20	20	45.0	45.0	57.7	58.1	0.0	8.9	0.313169	113275	113700	1083-1083	0.500000	57.5
ycf1-rps15_p4	ycf1-rps15	4	AGCCGAGTTTCACATGACAA	ATATTGCCCAGCTGTTCAGG	20	20	45.0	50.0	57.7	57.9	0.0	13.8	0.317516	113275	113700	1283-1283	0.500000	57.5
ycf1-rps15_p5	ycf1-rps15	5	AGCCGAGTTTCACATGACAA	CCAGCTGTTCAGGTCCATTT	20	20	45.0	50.0	57.7	58.1	0.0	13.8	0.339405	113275	113700	1276-1276	0.500000	57.3
ndhH_p1	ndhH	1	GCATGGAAGACTGACTCAGG	ATGTCTCACCGAATCACACG	20	20	55.0	50.0	58.0	58.0	10.9	0.0	0.025064	114088	115269	1783-1783	0.500000	59.8
ndhH_p2	ndhH	2	TTGGGAAAACGTCAACGACT	ATGTCTCACCGAATCACACG	20	20	45.0	50.0	58.0	58.0	9.9	0.0	0.030712	114088	115269	1974-1974	0.500000	59.8
ndhH_p3	ndhH	3	ATCTCAGAAAGGTTTGCGCA	ATGTCTCACCGAATCACACG	20	20	45.0	50.0	58.1	58.0	5.7	0.0	0.107489	114088	115269	1999-1999	0.500000	59.1
ndhH_p4	ndhH	4	ACGTCAACGACTACTAGCCT	ATGTCTCACCGAATCACACG	20	20	50.0	50.0	58.2	58.0	9.9	0.0	0.185066	114088	115269	1966-1966	0.500000	58.5
ndhH_p5	ndhH	5	GCATGGAAGACTGACTCAGG	AAGAATTCCCAAAGGACCCG	20	20	55.0	50.0	58.0	57.8	10.9	0.6	0.237297	114088	115269	1430-1430	0.500000	58.1
ndhA_p1	ndhA	1	AGAGTCGAAGCACCAAAAGG	CGTGGGACAGGGTAATCATG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.162206	115271	117373	2479-2479	0.500000	58.7
ndhA_p2	ndhA	2	AGTCGAAGCACCAAAAGGAG	CGTGGGACAGGGTAATCATG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.162206	115271	117373	2477-2477	0.500000	58.7
ndhA_p3	ndhA	3	GAGTCGAAGCACCAAAAGGA	CGTGGGACAGGGTAATCATG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.167488	115271	117373	2478-2478	0.500000	58.7
ndhA_p4	ndhA	4	GTCGAAGCACCAAAAGGAGA	CGTGGGACAGGGTAATCATG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.167488	115271	117373	2476-2476	0.500000	58.7
ndhA_p5	ndhA	5	TGATCGGGGATCAGAGTGTT	CGTGGGACAGGGTAATCATG	20	20	50.0	55.0	58.1	58.0	4.3	0.0	0.180426	115271	117373	2442-2442	0.500000	58.6
ndhI_p1	ndhI	1	ACGGTTCTGTAATAGCGACG	CCCAACGGTCCAAAGATGAA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.280098	117454	117960	1825-1825	0.500000	57.8
ndhI_p2	ndhI	2	GTGACCTATGAGGAGCCGTA	CCCAACGGTCCAAAGATGAA	20	20	55.0	50.0	58.3	58.1	0.0	0.0	0.406941	117454	117960	1864-1864	0.500000	56.7
ndhI_p3	ndhI	3	ACGGTTCTGTAATAGCGACG	AATCCCAATGAAAAGGCCGA	20	20	50.0	45.0	57.8	57.8	0.0	0.0	0.414514	117454	117960	1659-1659	0.500000	56.7
ndhI_p4	ndhI	4	ACGGTTCTGTAATAGCGACG	ATCCCAATGAAAAGGCCGAA	20	20	50.0	45.0	57.8	57.8	0.0	0.0	0.414514	117454	117960	1658-1658	0.500000	56.7
ndhI_p5	ndhI	5	ACGGTTCTGTAATAGCGACG	TCCCAATGAAAAGGCCGAAT	20	20	50.0	45.0	57.8	57.8	0.0	0.0	0.414514	117454	117960	1657-1657	0.500000	56.7
ndhI-ndhG_p1	ndhI-ndhG	1	CATGATTACCCTGTCCCACG	CCCAACGGTCCAAAGATGAA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.142154	117961	118322	1080-1080	0.500000	58.9
ndhI-ndhG_p2	ndhI-ndhG	2	ACATCAGAACGTTTCCGAGG	CCCAACGGTCCAAAGATGAA	20	20	50.0	50.0	57.9	58.1	0.5	0.0	0.175999	117961	118322	1013-1013	0.500000	58.6
ndhI-ndhG_p3	ndhI-ndhG	3	TCAAATTGCTTTAGGCCGGT	CCCAACGGTCCAAAGATGAA	20	20	45.0	50.0	58.1	58.1	1.9	0.0	0.177841	117961	118322	744-744	0.500000	58.6
ndhI-ndhG_p4	ndhI-ndhG	4	ACAAACAATACGAGCTGCCA	CCCAACGGTCCAAAGATGAA	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.204837	117961	118322	1119-1119	0.500000	58.4
ndhI-ndhG_p5	ndhI-ndhG	5	CTTGAACTCCGGTCGACAAA	CCCAACGGTCCAAAGATGAA	20	20	50.0	50.0	58.1	58.1	18.9	0.0	0.235037	117961	118322	1134-1134	0.500000	58.1
ndhG_p1	ndhG	1	ACATCAGAACGTTTCCGAGG	CCACGATCATAGCTTACCCG	20	20	50.0	55.0	57.9	57.9	0.5	0.0	0.146279	118323	118853	1857-1857	0.500000	58.8
ndhG_p2	ndhG	2	TCAAATTGCTTTAGGCCGGT	CCACGATCATAGCTTACCCG	20	20	45.0	55.0	58.1	57.9	1.9	0.0	0.148121	118323	118853	1588-1588	0.500000	58.8
ndhG_p3	ndhG	3	GAGGCCGAATCCACTTTGAA	CCACGATCATAGCTTACCCG	20	20	50.0	55.0	58.2	57.9	0.0	0.0	0.244776	118323	118853	1841-1841	0.500000	58.0
ndhG_p4	ndhG	4	ATTGCTTTAGGCCGGTTACC	CCACGATCATAGCTTACCCG	20	20	50.0	55.0	58.2	57.9	1.9	0.0	0.307056	118323	118853	1584-1584	0.500000	57.5
ndhG_p5	ndhG	5	ACATCAGAACGTTTCCGAGG	CCGATCCAATAGCTGCTTCA	20	20	50.0	50.0	57.9	57.8	0.5	7.8	0.325445	118323	118853	1699-1699	0.500000	57.4
ndhE_p1	ndhE	1	TTCATCTTTGGACCGTTGGG	CCACGATCATAGCTTACCCG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.161706	119062	119367	864-864	0.500000	58.7
ndhE_p2	ndhE	2	TTCATCTTTGGACCGTTGGG	ATACGCTTGGGCCAGAAAAA	20	20	50.0	45.0	58.1	58.1	0.0	6.2	0.177841	119062	119367	1382-1382	0.500000	58.6
ndhE_p3	ndhE	3	TTCATCTTTGGACCGTTGGG	ACCTCTTACAACCAACGCAG	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.216760	119062	119367	1191-1191	0.500000	58.3
ndhE_p4	ndhE	4	TTCATCTTTGGACCGTTGGG	CTAATACATCGGTGGGGCAC	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.216760	119062	119367	1110-1110	0.500000	58.3
ndhE_p5	ndhE	5	TTCATCTTTGGACCGTTGGG	CCTTGGGGCAGAAGCTATTT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.245686	119062	119367	1163-1163	0.500000	58.0
ndhE-psaC_p1	ndhE-psaC	1	TGAAGCAGCTATTGGATCGG	ATACGCTTGGGCCAGAAAAA	20	20	50.0	45.0	57.8	58.1	0.0	6.2	0.327287	119368	119621	696-696	0.500000	57.4
ndhE-psaC_p2	ndhE-psaC	2	TGAAGCAGCTATTGGATCGG	ACCTCTTACAACCAACGCAG	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.366206	119368	119621	505-505	0.500000	57.1
ndhE-psaC_p3	ndhE-psaC	3	TGAAGCAGCTATTGGATCGG	CTAATACATCGGTGGGGCAC	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.366206	119368	119621	424-424	0.500000	57.1
ndhE-psaC_p4	ndhE-psaC	4	TGAAGCAGCTATTGGATCGG	GAGAATCTCGAGTAACCGGC	20	20	50.0	55.0	57.8	57.9	0.0	13.1	0.367722	119368	119621	1042-1042	0.500000	57.1
ndhE-psaC_p5	ndhE-psaC	5	TGAAGCAGCTATTGGATCGG	CCTTGGGGCAGAAGCTATTT	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.395132	119368	119621	477-477	0.500000	56.8
ndhD_p1	ndhD	1	CGGGTAAGCTATGATCGTGG	CTAATGAAGCATGGGGGTCC	20	20	55.0	55.0	57.9	58.0	0.0	0.0	0.079110	119985	121487	2501-2501	0.500000	59.4
ndhD_p2	ndhD	2	CGGGTAAGCTATGATCGTGG	GAAGCATGGGGGTCCTATTG	20	20	55.0	55.0	57.9	58.0	0.0	0.0	0.079110	119985	121487	2496-2496	0.500000	59.4
ndhD_p3	ndhD	3	TTTTTCTGGCCCAAGCGTAT	CTAATGAAGCATGGGGGTCC	20	20	45.0	55.0	58.1	58.0	0.0	0.0	0.095246	119985	121487	1983-1983	0.500000	59.2
ndhD_p4	ndhD	4	TTTTTCTGGCCCAAGCGTAT	GAAGCATGGGGGTCCTATTG	20	20	45.0	55.0	58.1	58.0	0.0	0.0	0.095246	119985	121487	1978-1978	0.500000	59.2
ndhD_p5	ndhD	5	CTGCGTTGGTTGTAAGAGGT	GAAGCATGGGGGTCCTATTG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.134165	119985	121487	2169-2169	0.500000	58.9
ccsA_p1	ccsA	1	AACGGGGCCCTTTTACAAAT	TAGACACGCTGCTCTTAGGA	20	20	45.0	50.0	58.0	57.9	24.4	0.0	0.142632	121700	122671	1880-1880	0.500000	58.9
ccsA_p2	ccsA	2	AACGGGGCCCTTTTACAAAT	ACAAGCCGCTATGGTGAAAT	20	20	45.0	45.0	58.0	57.9	24.4	0.0	0.147126	121700	122671	1903-1903	0.500000	58.8
ccsA_p3	ccsA	3	AACGGGGCCCTTTTACAAAT	AAATTGGTAGACACGCTGCT	20	20	45.0	45.0	58.0	57.8	24.4	0.0	0.203359	121700	122671	1887-1887	0.500000	58.4
ccsA_p4	ccsA	4	AACGGGGCCCTTTTACAAAT	GGTAGACACGCTGCTCTTAG	20	20	45.0	55.0	58.0	57.8	24.4	0.0	0.214650	121700	122671	1882-1882	0.500000	58.3
ccsA_p5	ccsA	5	AACGGGGCCCTTTTACAAAT	GTAGACACGCTGCTCTTAGG	20	20	45.0	55.0	58.0	57.8	24.4	0.0	0.214650	121700	122671	1881-1881	0.500000	58.3
ccsA-trnL-UAG_p1	ccsA-trnL-UAG	1	AACCACTCGGATTGAAGAGC	TAGACACGCTGCTCTTAGGA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.224882	122672	122787	584-584	0.500000	58.2
ccsA-trnL-UAG_p2	ccsA-trnL-UAG	2	AACCACTCGGATTGAAGAGC	ACAAGCCGCTATGGTGAAAT	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.229375	122672	122787	607-607	0.500000	58.2
ccsA-trnL-UAG_p3	ccsA-trnL-UAG	3	AACCACTCGGATTGAAGAGC	AAATTGGTAGACACGCTGCT	20	20	50.0	45.0	57.9	57.8	0.0	0.0	0.285609	122672	122787	591-591	0.500000	57.7
ccsA-trnL-UAG_p4	ccsA-trnL-UAG	4	AACCACTCGGATTGAAGAGC	GGTAGACACGCTGCTCTTAG	20	20	50.0	55.0	57.9	57.8	0.0	0.0	0.296900	122672	122787	586-586	0.500000	57.6
ccsA-trnL-UAG_p5	ccsA-trnL-UAG	5	AACCACTCGGATTGAAGAGC	GTAGACACGCTGCTCTTAGG	20	20	50.0	55.0	57.9	57.8	0.0	0.0	0.296900	122672	122787	585-585	0.500000	57.6
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	AACCACTCGGATTGAAGAGC	CGGCGTTAAAAGCGTTTTCT	20	20	50.0	45.0	57.9	58.0	0.0	2.2	0.127520	122868	123627	1455-1455	0.500000	59.0
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	TCCTAAGAGCAGCGTGTCTA	CGGCGTTAAAAGCGTTTTCT	20	20	50.0	45.0	57.9	58.0	0.0	2.2	0.151192	122868	123627	891-891	0.500000	58.8
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	AGCAGCGTGTCTACCAATTT	CGGCGTTAAAAGCGTTTTCT	20	20	45.0	45.0	57.8	58.0	0.0	2.2	0.211919	122868	123627	884-884	0.500000	58.3
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	CCTAAGAGCAGCGTGTCTAC	CGGCGTTAAAAGCGTTTTCT	20	20	55.0	45.0	57.8	58.0	0.0	2.2	0.223210	122868	123627	890-890	0.500000	58.2
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	CTAAGAGCAGCGTGTCTACC	CGGCGTTAAAAGCGTTTTCT	20	20	55.0	45.0	57.8	58.0	0.0	2.2	0.223210	122868	123627	889-889	0.500000	58.2
rpl32_p1	rpl32	1	GCTGGGGGAAATAAGACTCC	GCCCACATACGACGAAGATT	20	20	55.0	50.0	57.6	58.1	0.0	0.0	0.414125	123628	123798	1019-1019	0.500000	56.7
rpl32_p2	rpl32	2	CACTAAGCGTTTTTGCGTGA	GCCCACATACGACGAAGATT	20	20	45.0	50.0	57.6	58.1	0.0	0.0	0.440982	123628	123798	1154-1154	0.500000	56.5
rpl32_p3	rpl32	3	GCTGGGGGAAATAAGACTCC	AAAAGCCCACATACGACGAA	20	20	55.0	45.0	57.6	57.8	0.0	0.0	0.522908	123628	123798	1023-1023	0.500000	55.8
rpl32_p4	rpl32	4	GCTGGGGGAAATAAGACTCC	TGGAAAAGCCCACATACGAC	20	20	55.0	50.0	57.6	58.2	0.0	0.0	0.533983	123628	123798	1026-1026	0.500000	55.7
rpl32_p5	rpl32	5	TGCGTGAATTGATGGTGAGA	GCCCACATACGACGAAGATT	20	20	45.0	50.0	57.5	58.1	0.0	0.0	0.535051	123628	123798	1141-1141	0.500000	55.7
rpl32-ndhF_p1	rpl32-ndhF	1	AGAAAACGCTTTTAACGCCG	GCCCACATACGACGAAGATT	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.090982	123799	124276	699-699	0.500000	59.3
rpl32-ndhF_p2	rpl32-ndhF	2	AGAAAACGCTTTTAACGCCG	AAAAGCCCACATACGACGAA	20	20	45.0	45.0	58.0	57.8	0.0	0.0	0.199766	123799	124276	703-703	0.500000	58.4
rpl32-ndhF_p3	rpl32-ndhF	3	AGAAAACGCTTTTAACGCCG	TGGAAAAGCCCACATACGAC	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.210840	123799	124276	706-706	0.500000	58.3
rpl32-ndhF_p4	rpl32-ndhF	4	AGAAAACGCTTTTAACGCCG	AAAGCCCACATACGACGAAG	20	20	45.0	50.0	58.0	58.3	0.0	0.0	0.304904	123799	124276	702-702	0.500000	57.6
rpl32-ndhF_p5	rpl32-ndhF	5	AGAAAACGCTTTTAACGCCG	GAAAAGCCCACATACGACGA	20	20	45.0	50.0	58.0	58.3	0.0	0.0	0.309721	123799	124276	704-704	0.500000	57.5
ndhF_p1	ndhF	1	AGAAAACGCTTTTAACGCCG	GGCGGATCCGAACAAAATTG	20	20	45.0	50.0	58.0	58.1	0.0	37.6	0.118850	124277	126508	2967-2967	0.500000	59.0
ndhF_p2	ndhF	2	AGAAAACGCTTTTAACGCCG	AAGGCGGATCCGAACAAAAT	20	20	45.0	45.0	58.0	58.2	0.0	37.6	0.192727	124277	126508	2969-2969	0.500000	58.5
ndhF_p3	ndhF	3	AGAAAACGCTTTTAACGCCG	AGGCGGATCCGAACAAAATT	20	20	45.0	45.0	58.0	58.2	0.0	37.6	0.192727	124277	126508	2968-2968	0.500000	58.5
ndhF_p4	ndhF	4	AGAAAACGCTTTTAACGCCG	GAAGAAAAGGCGGATCCGAA	20	20	45.0	50.0	58.0	58.3	0.0	37.6	0.292752	124277	126508	2975-2975	0.500000	57.7
ndhF_p5	ndhF	5	AGAAAACGCTTTTAACGCCG	AAAGGCGGATCCGAACAAAA	20	20	45.0	45.0	58.0	58.4	0.0	37.6	0.415396	124277	126508	2970-2970	0.500000	56.7