primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	CGTTGCTTTCTACCACATCG	TAAGGCACCGAGTTTTGGTC	20	20	50.0	50.0	57.2	58.1	0.0	0.0	0.900836	3827	4694	963-991	1.000000	82.8
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	CGTTGCTTTCTACCACATCG	GTTTTGGTCTCGCTAGTCGA	20	20	50.0	50.0	57.2	57.7	0.0	8.6	1.130632	3827	4694	952-980	1.000000	81.1
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CGTTGCTTTCTACCACATCG	AGTTTTGGTCTCGCTAGTCG	20	20	50.0	50.0	57.2	57.7	0.0	0.0	1.137020	3827	4694	953-981	1.000000	81.0
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CGTTGCTTTCTACCACATCG	CGAGTTTTGGTCTCGCTAGT	20	20	50.0	50.0	57.2	57.7	0.0	2.6	1.137020	3827	4694	955-983	1.000000	81.0
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CGTTGCTTTCTACCACATCG	AAATTAAAATGGGGCGTGGC	20	20	50.0	45.0	57.2	57.6	0.0	0.0	1.182652	3827	4694	990-1018	1.000000	80.3
trnQ-UUG-trnS-GCU_p1	trnQ-UUG-trnS-GCU	1	GACCAAAACTCGGTGCCTTA	TTATTCGTACCGAGGGTTCG	20	20	50.0	50.0	58.1	57.2	0.0	7.4	0.962994	4767	5304	611-1181	1.000000	80.2
trnQ-UUG-trnS-GCU_p2	trnQ-UUG-trnS-GCU	2	TCGACTAGCGAGACCAAAAC	TTATTCGTACCGAGGGTTCG	20	20	50.0	50.0	57.7	57.2	0.0	7.4	1.192790	4767	5304	622-1192	1.000000	78.4
trnQ-UUG-trnS-GCU_p3	trnQ-UUG-trnS-GCU	3	GACCAAAACTCGGTGCCTTA	GGGTTCGAATCCCTCTCTTT	20	20	50.0	50.0	58.1	56.9	0.0	17.2	1.195668	4767	5304	598-1168	1.000000	78.4
trnQ-UUG-trnS-GCU_p4	trnQ-UUG-trnS-GCU	4	GACCAAAACTCGGTGCCTTA	GGTTCGAATCCCTCTCTTTCC	20	21	50.0	52.4	58.1	58.1	0.0	0.0	1.205479	4767	5304	597-1167	1.000000	78.4
trnQ-UUG-trnS-GCU_p5	trnQ-UUG-trnS-GCU	5	CGACTAGCGAGACCAAAACT	TTATTCGTACCGAGGGTTCG	20	20	50.0	50.0	57.7	57.2	0.0	7.4	1.199178	4767	5304	621-1191	1.000000	78.3
trnS-GCU-trnR-UCU_p1	trnS-GCU-trnR-UCU	1	CCAACGCTTTAGTCCACTCA	GGCTTAGAAGACCTCTGTCC	20	20	50.0	55.0	57.8	57.0	0.0	0.0	1.135478	5392	6188	869-1411	1.000000	81.9
trnS-GCU-trnR-UCU_p2	trnS-GCU-trnR-UCU	2	AACGCTTTAGTCCACTCAGC	GGCTTAGAAGACCTCTGTCC	20	20	50.0	55.0	58.2	57.0	2.0	0.0	1.174635	5392	6188	867-1409	1.000000	81.6
trnS-GCU-trnR-UCU_p3	trnS-GCU-trnR-UCU	3	GCTTTAGTCCACTCAGCCAT	GGCTTAGAAGACCTCTGTCC	20	20	50.0	55.0	57.6	57.0	0.9	0.0	1.381608	5392	6188	864-1406	1.000000	79.9
trnS-GCU-trnR-UCU_p4	trnS-GCU-trnR-UCU	4	TCCAACGCTTTAGTCCACTC	GGCTTAGAAGACCTCTGTCC	20	20	50.0	55.0	57.5	57.0	0.0	0.0	1.422269	5392	6188	870-1412	1.000000	79.6
trnS-GCU-trnR-UCU_p5	trnS-GCU-trnR-UCU	5	AGTCCACTCAGCCATCTCTC	GGCTTAGAAGACCTCTGTCC	20	20	55.0	55.0	58.5	57.0	0.0	0.0	1.487622	5392	6188	859-1401	1.000000	79.1
atpH-atpI_p1	atpH-atpI	1	AACGGAAGCAGCAGAAATCA	GAGTTAGCCGCACCTACAAA	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.192730	9773	10305	923-932	1.000000	86.2
atpH-atpI_p2	atpH-atpI	2	AGCCAATCCAGCAGCAATAA	GAGTTAGCCGCACCTACAAA	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.237395	9773	10305	941-950	1.000000	85.7
atpH-atpI_p3	atpH-atpI	3	GCCAATCCAGCAGCAATAAC	GAGTTAGCCGCACCTACAAA	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.303337	9773	10305	940-949	1.000000	85.2
atpH-atpI_p4	atpH-atpI	4	AACGGAAGCAGCAGAAATCA	ATCCACAAACCATTCCGACC	20	20	45.0	50.0	58.1	58.2	0.0	0.0	0.269592	9773	10305	1120-1129	1.000000	84.5
atpH-atpI_p5	atpH-atpI	5	AGCAGTTCCTTGACCAATCC	GAGTTAGCCGCACCTACAAA	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.295526	9773	10305	995-995	0.333333	51.0
trnC-GCA-trnD-GUC_p1	trnC-GCA-trnD-GUC	1	CCAGTTCAAATCCGGGTGTC	CCTGTCAAGGCGGAAGTTG	20	19	55.0	57.9	58.8	58.8	0.0	0.0	2.586940	12453	13428	1043-2287	1.000000	67.1
trnC-GCA-trnD-GUC_p2	trnC-GCA-trnD-GUC	2	CAGTTCAAATCCGGGTGTCG	CCTGTCAAGGCGGAAGTTG	20	19	55.0	57.9	59.2	58.8	0.0	0.0	2.955898	12453	13428	1042-2286	1.000000	64.1
trnC-GCA-trnD-GUC_p3	trnC-GCA-trnD-GUC	3	AGTTCAAATCCGGGTGTCG	CCTGTCAAGGCGGAAGTTG	19	19	52.6	57.9	57.8	58.8	0.0	0.0	2.979094	12453	13428	1041-2285	1.000000	64.0
trnC-GCA-trnD-GUC_p4	trnC-GCA-trnD-GUC	4	CCAGTTCAAATCCGGGTGTC	TGTCAAGGCGGAAGTTGC	20	18	55.0	55.6	58.8	58.3	0.0	0.0	3.090171	12453	13428	1041-2285	1.000000	63.1
trnC-GCA-trnD-GUC_p5	trnC-GCA-trnD-GUC	5	CCAGTTCAAATCCGGGTGT	CCTGTCAAGGCGGAAGTTG	19	19	52.6	57.9	57.7	58.8	0.0	0.0	3.091757	12453	13428	1043-2287	1.000000	63.1
trnT-GGU-trnS-UGA_p1	trnT-GGU-trnS-UGA	1	GGTAAGGCGTAGGTCATTGG	GAGAAGCAAATACGACGGGT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.056505	14468	15968	1332-2767	1.000000	91.5
trnT-GGU-trnS-UGA_p2	trnT-GGU-trnS-UGA	2	GTGGTAGAGTAACGCCATGG	GAGAAGCAAATACGACGGGT	20	20	55.0	50.0	58.1	58.0	7.6	0.0	0.070038	14468	15968	1350-2785	1.000000	91.4
trnT-GGU-trnS-UGA_p3	trnT-GGU-trnS-UGA	3	TCAGTGGTAGAGTAACGCCA	GAGAAGCAAATACGACGGGT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.092193	14468	15968	1353-2788	1.000000	91.3
trnT-GGU-trnS-UGA_p4	trnT-GGU-trnS-UGA	4	AAGGCGTAGGTCATTGGTTC	GAGAAGCAAATACGACGGGT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.115395	14468	15968	1329-2764	1.000000	91.1
trnT-GGU-trnS-UGA_p5	trnT-GGU-trnS-UGA	5	GGTAAGGCGTAGGTCATTGG	TCTATTTGTGCAGTGTCGGG	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.143841	14468	15968	1613-2276	0.666667	73.9
trnS-UGA-trnG-UCC_p1	trnS-UGA-trnG-UCC	1	AACCTCTCAGCCATCTCTCC	TGGCAAAGAGAAATTTTACCA	20	21	55.0	33.3	58.5	53.9	0.0	0.0	5.628415	16055	16762	753-788	1.000000	49.4
trnS-UGA-trnG-UCC_p2	trnS-UGA-trnG-UCC	2	AACCTCTCAGCCATCTCTCC	TTGGCAAAGAGAAATTTTACCA	20	22	55.0	31.8	58.5	54.7	0.0	0.0	5.787702	16055	16762	754-789	1.000000	49.4
trnS-UGA-trnG-UCC_p3	trnS-UGA-trnG-UCC	3	AACCTCTCAGCCATCTCTCC	TGGCAAAGAGAAATTTTACCAT	20	22	55.0	31.8	58.5	54.5	0.0	0.0	6.007144	16055	16762	753-788	1.000000	49.4
trnS-UGA-trnG-UCC_p4	trnS-UGA-trnG-UCC	4	AACCTCTCAGCCATCTCTCC	TGGCAAAGAGAAATTTTACCATT	20	23	55.0	30.4	58.5	55.3	0.0	0.0	6.227410	16055	16762	753-788	1.000000	49.4
trnS-UGA-trnG-UCC_p5	trnS-UGA-trnG-UCC	5	AACCTCTCAGCCATCTCTCC	TTGGCAAAGAGAAATTTTACCAT	20	23	55.0	30.4	58.5	55.3	0.0	0.0	6.227410	16055	16762	754-789	1.000000	49.4
trnfM-CAU-rps14_p1	trnfM-CAU-rps14	1	AAGGTTATGAGCCTTGCGAG	GCATGTTTGTTACCAGGAGC	20	20	50.0	50.0	58.0	57.4	0.0	0.0	0.666606	17085	17232	220-230	1.000000	86.7
trnfM-CAU-rps14_p2	trnfM-CAU-rps14	2	GATGTTGCGGAGACAGGATT	GCATGTTTGTTACCAGGAGC	20	20	50.0	50.0	58.0	57.4	0.0	0.0	0.666606	17085	17232	255-265	1.000000	85.8
trnfM-CAU-rps14_p3	trnfM-CAU-rps14	3	CCAAACTACTCTACTCCGCG	GCATGTTTGTTACCAGGAGC	20	20	55.0	50.0	57.8	57.4	0.0	0.0	0.827093	17085	17232	197-207	1.000000	85.4
trnfM-CAU-rps14_p4	trnfM-CAU-rps14	4	TGCGGAGACAGGATTTGAAC	GCATGTTTGTTACCAGGAGC	20	20	50.0	50.0	58.2	57.4	0.0	0.0	0.828357	17085	17232	250-260	1.000000	84.7
trnfM-CAU-rps14_p5	trnfM-CAU-rps14	5	AAAGATGTTGCGGAGACAGG	GCATGTTTGTTACCAGGAGC	20	20	50.0	50.0	58.2	57.4	0.0	0.0	0.823255	17085	17232	258-268	1.000000	84.4
rps14-trnS-GGA_p1	rps14-trnS-GGA	1	TGCCTCTTCTTTTCCTTCTGA	TTCCAATGCTACGCCTTGAA	21	20	42.9	45.0	56.9	57.8	0.0	5.6	2.337743	17536	19731	1260-2288	1.000000	73.3
rps14-trnS-GGA_p2	rps14-trnS-GGA	2	TGCCTCTTCTTTTCCTTCTGAA	TTCCAATGCTACGCCTTGAA	22	20	40.9	45.0	57.6	57.8	0.0	5.6	2.630345	17536	19731	1260-2288	1.000000	71.0
rps14-trnS-GGA_p3	rps14-trnS-GGA	3	TTGCCTCTTCTTTTCCTTCTGA	TTCCAATGCTACGCCTTGAA	22	20	40.9	45.0	57.6	57.8	0.0	5.6	2.630345	17536	19731	1261-2289	1.000000	71.0
rps14-trnS-GGA_p4	rps14-trnS-GGA	4	TGCCTCTTCTTTTCCTTCTGA	TCCAATGCTACGCCTTGAAC	21	20	42.9	50.0	56.9	58.6	0.0	5.6	2.696095	17536	19731	1259-2287	1.000000	70.4
rps14-trnS-GGA_p5	rps14-trnS-GGA	5	TGCCTCTTCTTTTCCTTCTG	TTCCAATGCTACGCCTTGAA	20	20	45.0	45.0	55.3	57.8	0.0	5.6	2.920867	17536	19731	1260-2288	1.000000	68.6
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	20999	21382	445-480	1.000000	88.1
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	20999	21382	439-474	1.000000	86.9
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	CGTAGCGTCTACCGATTTCG	20	20	55.0	55.0	57.9	58.6	0.0	10.2	0.749620	20999	21382	444-479	1.000000	85.2
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	TCCGTAGCGTCTACCGATTT	20	20	55.0	50.0	57.9	58.6	0.0	10.2	0.750218	20999	21382	446-481	1.000000	85.2
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	ATGCGATGCTCTAACCTCTG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.5	58.2	0.0	10.2	0.771284	20999	21382	447-482	1.000000	85.0
trnF-GAA-trnM-CAU_p1	trnF-GAA-trnM-CAU	1	ATGGTCGGGATAGCTCAGTT	CCCGCCGTATGAAAGCAATA	20	20	50.0	50.0	58.2	58.4	0.0	0.0	0.616612	22401	22829	548-1552	1.000000	79.2
trnF-GAA-trnM-CAU_p2	trnF-GAA-trnM-CAU	2	AATGGTCGGGATAGCTCAGT	CCCGCCGTATGAAAGCAATA	20	20	50.0	50.0	58.2	58.4	0.0	0.0	0.616612	22401	22829	549-1553	1.000000	79.1
trnF-GAA-trnM-CAU_p3	trnF-GAA-trnM-CAU	3	AATGGTCGGGATAGCTCAGT	CCGCCGTATGAAAGCAATAC	20	20	50.0	50.0	58.2	57.3	0.0	0.0	0.868565	22401	22829	548-1552	1.000000	77.1
trnF-GAA-trnM-CAU_p4	trnF-GAA-trnM-CAU	4	ATGGTCGGGATAGCTCAGTT	CCGCCGTATGAAAGCAATAC	20	20	50.0	50.0	58.2	57.3	0.0	0.0	0.868565	22401	22829	547-1551	1.000000	77.1
trnF-GAA-trnM-CAU_p5	trnF-GAA-trnM-CAU	5	TCGGGATAGCTCAGTTGGTA	CCCGCCGTATGAAAGCAATA	20	20	50.0	50.0	57.6	58.4	0.0	0.0	0.858734	22401	22829	544-1548	0.666667	63.6
trnM-CAU-atpB_p1	trnM-CAU-atpB	1	GCGGGAGTCATTGGTTCAAA	GATGAAGCTATTGCGAAAG	20	19	50.0	42.1	58.8	52.1	0.0	0.0	7.649564	22902	23535	673-701	1.000000	50.8
trnM-CAU-atpB_p2	trnM-CAU-atpB	2	GCGGGAGTCATTGGTTCAA	GATGAAGCTATTGCGAAAG	19	19	52.6	42.1	58.1	52.1	0.0	0.0	7.952256	22902	23535	673-701	1.000000	50.8
trnM-CAU-atpB_p3	trnM-CAU-atpB	3	CTTTCATACGGCGGGAGTC	GATGAAGCTATTGCGAAAG	19	19	57.9	42.1	58.0	52.1	13.2	0.0	7.920046	22902	23535	683-711	1.000000	50.6
trnM-CAU-atpB_p4	trnM-CAU-atpB	4	TATTGCTTTCATACGGCGGG	GATGAAGCTATTGCGAAAG	20	19	50.0	42.1	58.4	52.1	13.2	0.0	7.304792	22902	23535	688-716	1.000000	50.5
trnM-CAU-atpB_p5	trnM-CAU-atpB	5	GTATTGCTTTCATACGGCGG	GATGAAGCTATTGCGAAAG	20	19	50.0	42.1	57.3	52.1	13.2	0.0	7.556745	22902	23535	689-717	1.000000	50.5
clpP-rpl36_p1	clpP-rpl36	1	AAAGGCCAGGGAGTGCTATA	ATCGGGAAGCTCGACTAGAA	20	20	50.0	50.0	58.1	57.9	5.0	0.0	0.160917	32728	34352	2477-2484	0.666667	73.5
clpP-rpl36_p2	clpP-rpl36	2	GCCTTTGACCCTCGTATAGC	ATCGGGAAGCTCGACTAGAA	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.187066	32728	34352	2385-2392	0.666667	73.3
clpP-rpl36_p3	clpP-rpl36	3	CCTAGTCCAATCGCAAGCTT	ATCGGGAAGCTCGACTAGAA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.084070	32728	34352	2362-2362	0.333333	52.7
clpP-rpl36_p4	clpP-rpl36	4	GGTAGTGACCTCTGCCTTTG	ATCGGGAAGCTCGACTAGAA	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.154837	32728	34352	2405-2405	0.333333	52.1
clpP-rpl36_p5	clpP-rpl36	5	CCTAGTCCAATCGCAAGCTT	AGTCGACCTGGTCTACGAAT	20	20	50.0	50.0	58.0	57.9	0.0	23.5	0.155611	32728	34352	2465-2465	0.333333	52.1
trnA-UGC_p1	trnA-UGC	1	GACCTCGAGCACAGGTTTAG	ATTCGGGAATCTCCGGATCT	20	20	55.0	50.0	58.0	58.0	7.0	3.6	0.019236	54977	55852	920-1610	0.666667	71.4
trnA-UGC_p2	trnA-UGC	2	GACCTCGAGCACAGGTTTAG	TCGAAAGGTTGCCCTATTCG	20	20	55.0	50.0	58.0	58.0	7.0	14.3	0.020873	54977	55852	935-1625	0.666667	71.4
trnA-UGC_p3	trnA-UGC	3	GACCTCGAGCACAGGTTTAG	CTCCCACAACCCCGTTTTAA	20	20	55.0	50.0	58.0	58.0	7.0	0.0	0.018265	54977	55852	1082-1772	0.666667	71.3
trnA-UGC_p4	trnA-UGC	4	TGATTTCCTTCACGAGCGAG	CTCCCACAACCCCGTTTTAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.019643	54977	55852	1436-1436	0.333333	53.2
trnA-UGC_p5	trnA-UGC	5	TGATTTCCTTCACGAGCGAG	ATTCGGGAATCTCCGGATCT	20	20	50.0	50.0	58.0	58.0	0.0	3.6	0.020613	54977	55852	1274-1274	0.333333	53.2
trnL-UAG-rps15_p1	trnL-UAG-rps15	1	TAGACACGCTGCTCTTAGGA	TCTCAGATAGGTTTGCGCAA	20	20	50.0	45.0	57.9	57.2	0.0	21.0	0.884912	61782	64046	1650-2533	1.000000	84.9
trnL-UAG-rps15_p2	trnL-UAG-rps15	2	ACAAGCCGCTATGGTGAAAT	TCTCAGATAGGTTTGCGCAA	20	20	45.0	45.0	57.9	57.2	0.0	21.0	0.889405	61782	64046	1673-2556	1.000000	84.9
trnL-UAG-rps15_p3	trnL-UAG-rps15	3	AAATTGGTAGACACGCTGCT	TCTCAGATAGGTTTGCGCAA	20	20	45.0	45.0	57.8	57.2	0.0	21.0	0.945639	61782	64046	1657-2540	1.000000	84.4
trnL-UAG-rps15_p4	trnL-UAG-rps15	4	GGTAGACACGCTGCTCTTAG	TCTCAGATAGGTTTGCGCAA	20	20	55.0	45.0	57.8	57.2	0.0	21.0	0.956929	61782	64046	1652-2535	1.000000	84.3
trnL-UAG-rps15_p5	trnL-UAG-rps15	5	GTAGACACGCTGCTCTTAGG	TCTCAGATAGGTTTGCGCAA	20	20	55.0	45.0	57.8	57.2	0.0	21.0	0.956929	61782	64046	1651-2534	1.000000	84.3
rps15-rps12_p1	rps15-rps12	1	CTCCCACAACCCCGTTTTAA	GGCTGTCAAGAACGTAGAGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.018265	74213	74812	1141-1141	0.333333	53.2
rps15-rps12_p2	rps15-rps12	2	CTCCCACAACCCCGTTTTAA	GGGCTGTCAAGAACGTAGAG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.018265	74213	74812	1142-1142	0.333333	53.2
rps15-rps12_p3	rps15-rps12	3	ATTCGGGAATCTCCGGATCT	GGCTGTCAAGAACGTAGAGG	20	20	50.0	55.0	58.0	58.0	3.6	0.0	0.019236	74213	74812	979-979	0.333333	53.2
rps15-rps12_p4	rps15-rps12	4	ATTCGGGAATCTCCGGATCT	GGGCTGTCAAGAACGTAGAG	20	20	50.0	55.0	58.0	58.0	3.6	0.0	0.019236	74213	74812	980-980	0.333333	53.2
rps15-rps12_p5	rps15-rps12	5	CTCCCACAACCCCGTTTTAA	TGATTTCCTTCACGAGCGAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.019643	74213	74812	1436-1436	0.333333	53.2