primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU_p1	trnK-UUU	1	TGCTAATGTCAACCAATCAA	CAGTTGTGCAGTGCAATTTCA	20	21	35.0	42.9	53.1	58.2	0.0	21.7	6.118252	1345	4203	2997-2998	1.000000	40.1
trnK-UUU_p2	trnK-UUU	2	TGCTAATGTCAACCAATCAA	AGTTGTGCAGTGCAATTTCA	20	20	35.0	40.0	53.1	56.7	0.0	21.7	6.183026	1345	4203	2996-2997	1.000000	40.1
trnK-UUU_p3	trnK-UUU	3	TGCTAATGTCAACCAATCAAA	CAGTTGTGCAGTGCAATTTCA	21	21	33.3	42.9	54.0	58.2	0.0	21.7	6.204501	1345	4203	2997-2998	1.000000	40.1
trnK-UUU_p4	trnK-UUU	4	TGCTAATGTCAACCAATCAAA	AGTTGTGCAGTGCAATTTCA	21	20	33.3	40.0	54.0	56.7	0.0	21.7	6.269275	1345	4203	2996-2997	1.000000	40.1
trnK-UUU_p5	trnK-UUU	5	ATGCTAATGTCAACCAATCA	CAGTTGTGCAGTGCAATTTCA	20	21	35.0	42.9	52.8	58.2	0.0	21.7	6.364707	1345	4203	2998-2999	1.000000	40.1
matK_p1	matK	1	GCACACGACTTTCCCTATGT	TACACATACGCCGTTCTGAC	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.361950	1572	3122	1764-1764	1.000000	77.1
matK_p2	matK	2	GCACACGACTTTCCCTATGT	ACGCCGTTCTGACCATATTG	20	20	50.0	50.0	57.9	58.3	0.0	0.0	0.442775	1572	3122	1757-1757	1.000000	76.5
matK_p3	matK	3	GCACACGACTTTCCCTATGT	CATACGCCGTTCTGACCATA	20	20	50.0	50.0	57.9	57.5	0.0	0.0	0.596975	1572	3122	1760-1760	1.000000	75.3
matK_p4	matK	4	GCACACGACTTTCCCTATGT	GCCGTTCTGACCATATTGCA	20	20	50.0	50.0	57.9	58.6	0.0	0.0	0.716232	1572	3122	1755-1755	1.000000	74.3
matK_p5	matK	5	GCACACGACTTTCCCTATGT	CATACGCCGTTCTGACCATAT	20	21	50.0	47.6	57.9	58.0	0.0	0.0	1.121392	1572	3122	1760-1760	1.000000	71.1
matK-trnK-UUU_p1	matK-trnK-UUU	1	TCCTGAAGGAGTAGCGGATA	ATCCGGGTTGCTAACTCAAC	20	20	50.0	50.0	57.0	57.9	0.0	0.0	1.153891	3123	4166	1168-1169	1.000000	70.9
matK-trnK-UUU_p2	matK-trnK-UUU	2	TCCTGAAGGAGTAGCGGATA	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	57.0	58.2	0.0	17.1	1.212886	3123	4166	1151-1152	1.000000	70.4
matK-trnK-UUU_p3	matK-trnK-UUU	3	TCCTGAAGGAGTAGCGGATA	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	57.0	58.2	0.0	17.1	1.212886	3123	4166	1150-1151	1.000000	70.4
matK-trnK-UUU_p4	matK-trnK-UUU	4	TCCTGAAGGAGTAGCGGATA	GATCCGGGTTGCTAACTCAA	20	20	50.0	50.0	57.0	57.6	0.0	0.0	1.441056	3123	4166	1169-1170	1.000000	68.6
matK-trnK-UUU_p5	matK-trnK-UUU	5	TCCTGAAGGAGTAGCGGATA	TTGATCCGGGTTGCTAACTC	20	20	50.0	50.0	57.0	57.6	0.0	0.0	1.441056	3123	4166	1171-1172	1.000000	68.6
trnG-UCC_p1	trnG-UCC	1	AAATACGTGTGAATGCCGGA	TGCATGTTAAAATGAATAGCATCT	20	24	45.0	29.2	57.9	55.5	5.0	10.0	6.630696	8318	9077	937-946	1.000000	40.8
trnG-UCC_p2	trnG-UCC	2	AATACGTGTGAATGCCGGAA	AGATGCATGTTAAAATGAATAGCA	20	24	45.0	29.2	57.9	55.5	5.0	2.5	6.630696	8318	9077	939-948	1.000000	40.8
trnG-UCC_p3	trnG-UCC	3	AATACGTGTGAATGCCGGAA	TGCATGTTAAAATGAATAGCATCT	20	24	45.0	29.2	57.9	55.5	5.0	10.0	6.630696	8318	9077	936-945	1.000000	40.8
trnG-UCC_p4	trnG-UCC	4	TACGTGTGAATGCCGGAATT	AGATGCATGTTAAAATGAATAGCA	20	24	45.0	29.2	57.9	55.5	7.6	2.5	6.630696	8318	9077	937-946	1.000000	40.8
trnG-UCC_p5	trnG-UCC	5	TACGTGTGAATGCCGGAATT	TGCATGTTAAAATGAATAGCATCT	20	24	45.0	29.2	57.9	55.5	7.6	10.0	6.630696	8318	9077	934-943	1.000000	40.8
rps2_p1	rps2	1	TCAAAGAAAGTTCACCAAAAACGA	ATCCTGGTAGAAGGTTCCGT	24	20	33.3	50.0	58.0	57.8	0.0	0.0	4.264336	14331	15041	855-855	1.000000	46.0
rps2_p2	rps2	2	AGAAAGTTCACCAAAAACGAAC	ATCCTGGTAGAAGGTTCCGT	22	20	36.4	50.0	56.0	57.8	0.0	0.0	4.279763	14331	15041	851-851	1.000000	45.9
rps2_p3	rps2	3	TCACCAAAAACGAACTAATTGGA	ATCCTGGTAGAAGGTTCCGT	23	20	34.8	50.0	56.9	57.8	9.6	0.0	4.340742	14331	15041	844-844	1.000000	45.4
rps2_p4	rps2	4	TCAAAGAAAGTTCACCAAAAACGA	TCCTGGTAGAAGGTTCCGTC	24	20	33.3	55.0	58.0	58.4	0.0	0.0	4.461320	14331	15041	854-854	1.000000	44.4
rps2_p5	rps2	5	AGAAAGTTCACCAAAAACGAAC	TCCTGGTAGAAGGTTCCGTC	22	20	36.4	55.0	56.0	58.4	0.0	0.0	4.476748	14331	15041	850-850	1.000000	44.3
rpoC1_p1	rpoC1	1	ACACGAGTATGGGCATCACT	TTGCTCGTTCGAGAACTACG	20	20	50.0	50.0	58.8	58.0	0.0	23.5	0.845138	19613	22403	2971-2971	1.000000	73.3
rpoC1_p2	rpoC1	2	TACACGAGTATGGGCATCAC	TTGCTCGTTCGAGAACTACG	20	20	50.0	50.0	57.1	58.0	0.0	23.5	0.923180	19613	22403	2972-2972	1.000000	72.6
rpoC1_p3	rpoC1	3	TTACACGAGTATGGGCATCAC	TTGCTCGTTCGAGAACTACG	21	20	47.6	50.0	57.8	58.0	0.0	23.5	1.213921	19613	22403	2973-2973	1.000000	70.3
rpoC1_p4	rpoC1	4	CACGAGTATGGGCATCACTA	TTGCTCGTTCGAGAACTACG	20	20	50.0	50.0	56.8	58.0	0.0	23.5	1.218068	19613	22403	2970-2970	1.000000	70.3
rpoC1_p5	rpoC1	5	ACACGAGTATGGGCATCACT	GCTCGTTCGAGAACTACGAT	20	20	50.0	50.0	58.8	57.5	0.0	23.5	1.260180	19613	22403	2969-2969	1.000000	69.9
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	AATTCTTCCGTCAAGCCCTG	TAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.2	57.5	0.0	14.6	0.698720	25643	26857	1363-1363	1.000000	74.5
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	AATTCTTCCGTCAAGCCCTG	CGACACCCAGATTCGAACTG	20	20	50.0	55.0	58.2	58.6	0.0	16.2	0.823422	25643	26857	1387-1387	1.000000	73.5
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	CCGGGTATTGTGGACATTCC	TAAAGGATTTGCAGTCCCCC	20	20	55.0	50.0	58.3	57.5	0.0	14.6	0.849553	25643	26857	1300-1300	1.000000	73.3
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	CGGGTATTGTGGACATTCCC	TAAAGGATTTGCAGTCCCCC	20	20	55.0	50.0	58.3	57.5	0.0	14.6	0.849553	25643	26857	1299-1299	1.000000	73.3
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	AATTCTTCCGTCAAGCCCTG	TTTGTTGATCAGGCGACACC	20	20	50.0	50.0	58.2	58.8	0.0	0.0	0.938794	25643	26857	1400-1400	1.000000	72.6
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	ATGAACAGCGCAGTAGCAAT	AGTTCAATCGGTCAGAGCAC	20	20	45.0	50.0	58.3	57.9	11.5	0.0	0.343293	28541	29355	925-925	1.000000	77.3
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	TTTTGGCTGGCTGTTTTGAC	AGTTCAATCGGTCAGAGCAC	20	20	45.0	50.0	57.7	57.9	2.0	0.0	0.393988	28541	29355	979-979	1.000000	76.9
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	TGAACAGCGCAGTAGCAATA	AGTTCAATCGGTCAGAGCAC	20	20	45.0	50.0	57.6	57.9	11.5	0.0	0.464499	28541	29355	924-924	1.000000	76.4
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AAATGAACAGCGCAGTAGCA	AGTTCAATCGGTCAGAGCAC	20	20	45.0	50.0	58.5	57.9	11.5	0.0	0.563386	28541	29355	927-927	1.000000	75.6
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AATGAACAGCGCAGTAGCAA	AGTTCAATCGGTCAGAGCAC	20	20	45.0	50.0	58.5	57.9	11.5	0.0	0.563386	28541	29355	926-926	1.000000	75.6
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	GACCAGCCTACAAACACGAA	20	20	55.0	50.0	58.1	58.1	7.6	0.0	0.190370	30848	31856	1168-1168	1.000000	78.5
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	GACCAGCCTACAAACACGAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.212524	30848	31856	1171-1171	1.000000	78.4
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	CCCTCCGTAACCAATCATCC	20	20	55.0	55.0	58.1	57.7	7.6	0.0	0.320185	30848	31856	1143-1143	1.000000	77.5
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	TCAGTGGTAGAGTAACGCCA	CCCTCCGTAACCAATCATCC	20	20	50.0	55.0	58.1	57.7	0.0	0.0	0.342339	30848	31856	1146-1146	1.000000	77.3
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GTGGTAGAGTAACGCCATGG	CAGCCTACAAACACGAAACG	20	20	55.0	50.0	58.1	57.7	7.6	0.0	0.358546	30848	31856	1165-1165	1.000000	77.2
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	44964	45958	1044-1045	1.000000	79.0
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	CCGAGGCTCAATCCAATCAA	20	20	55.0	50.0	57.9	58.0	0.0	16.0	0.175426	44964	45958	1077-1078	1.000000	78.7
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	44964	45958	1052-1053	1.000000	78.5
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	44964	45958	1076-1077	1.000000	78.1
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	CCGAGGCTCAATCCAATCAA	20	20	50.0	50.0	57.8	58.0	15.5	16.0	0.283179	44964	45958	1109-1110	1.000000	77.8
accD_p1	accD	1	ATAGGGGGCATAAAGACTCT	ACACCATCTTCTTCGTTTTGT	20	21	45.0	38.1	54.9	56.0	0.0	0.0	6.034378	55931	57379	1579-1579	1.000000	40.1
accD_p2	accD	2	AATAGGGGGCATAAAGACTCT	ACACCATCTTCTTCGTTTTGT	21	21	42.9	38.1	55.8	56.0	0.0	0.0	6.185632	55931	57379	1580-1580	1.000000	40.1
accD_p3	accD	3	ATAGGGGGCATAAAGACTCT	AACACCATCTTCTTCGTTTTGT	20	22	45.0	36.4	54.9	56.8	0.0	0.0	6.300847	55931	57379	1580-1580	1.000000	40.1
accD_p4	accD	4	ATAGGGGGCATAAAGACTCT	ACACCATCTTCTTCGTTTTGTT	20	22	45.0	36.4	54.9	56.8	0.0	0.0	6.300847	55931	57379	1579-1579	1.000000	40.1
accD_p5	accD	5	AAATAGGGGGCATAAAGACTCT	ACACCATCTTCTTCGTTTTGT	22	21	40.9	38.1	56.6	56.0	0.0	0.0	6.415835	55931	57379	1581-1581	1.000000	40.1
psaJ-rpl33_p1	psaJ-rpl33	1	TTGGGTCTTTAGCGGGTCTA	AGAAATGCCCGTCGATTCTT	20	20	50.0	45.0	58.1	57.6	0.0	0.0	0.461875	64919	65477	749-756	1.000000	77.0
psaJ-rpl33_p2	psaJ-rpl33	2	GATGCCTTGTCATTCCCCTT	AGAAATGCCCGTCGATTCTT	20	20	50.0	45.0	57.9	57.6	0.0	0.0	0.553225	64919	65477	702-709	1.000000	76.4
psaJ-rpl33_p3	psaJ-rpl33	3	ATGGTTTGGGTCTTTAGCGG	AGAAATGCCCGTCGATTCTT	20	20	50.0	45.0	58.2	57.6	0.0	0.0	0.569064	64919	65477	754-761	1.000000	76.2
psaJ-rpl33_p4	psaJ-rpl33	4	TGGGTCTTTAGCGGGTCTAT	AGAAATGCCCGTCGATTCTT	20	20	50.0	45.0	57.8	57.6	0.0	0.0	0.573264	64919	65477	748-755	1.000000	76.2
psaJ-rpl33_p5	psaJ-rpl33	5	TGCCTTGTCATTCCCCTTTT	AGAAATGCCCGTCGATTCTT	20	20	45.0	45.0	57.6	57.6	0.0	0.0	0.800296	64919	65477	700-707	1.000000	74.4
rpl20-rps12_p1	rpl20-rps12	1	GTCAGTCTTGAATGAGCCCC	GAAATCTCCCGCTCTTCGAG	20	20	55.0	55.0	58.3	58.2	0.8	0.0	0.409463	66794	67576	935-936	1.000000	76.8
rpl20-rps12_p2	rpl20-rps12	2	TCAGTCTTGAATGAGCCCCT	GAAATCTCCCGCTCTTCGAG	20	20	50.0	55.0	58.3	58.2	0.0	0.0	0.491996	66794	67576	934-935	1.000000	76.1
rpl20-rps12_p3	rpl20-rps12	3	GTCAGTCTTGAATGAGCCCC	TAGAAACGCAAGACAGCCAA	20	20	55.0	45.0	58.3	57.8	0.8	0.0	0.504284	66794	67576	968-969	1.000000	76.0
rpl20-rps12_p4	rpl20-rps12	4	GTCAGTCTTGAATGAGCCCC	TTAGAAACGCAAGACAGCCA	20	20	55.0	45.0	58.3	57.8	0.8	0.0	0.504284	66794	67576	969-970	1.000000	76.0
rpl20-rps12_p5	rpl20-rps12	5	TCAGTCTTGAATGAGCCCCT	TAGAAACGCAAGACAGCCAA	20	20	50.0	45.0	58.3	57.8	0.0	0.0	0.586817	66794	67576	967-968	1.000000	75.4
trnH-GUG-rpl2_p1	trnH-GUG-rpl2	1	CGGATGTAGCCAAGTGGATC	GTTATCCTGCGCTTGGAAGA	20	20	55.0	50.0	58.1	58.0	0.2	22.4	0.153095	83407	83449	193-193	1.000000	79.2
trnH-GUG-rpl2_p2	trnH-GUG-rpl2	2	TGGATCAAGGCAGTGGATTG	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.157457	83407	83449	179-179	1.000000	79.2
trnH-GUG-rpl2_p3	trnH-GUG-rpl2	3	GGATCAAGGCAGTGGATTGT	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.158965	83407	83449	178-178	1.000000	79.2
trnH-GUG-rpl2_p4	trnH-GUG-rpl2	4	GTGGATCAAGGCAGTGGATT	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.158965	83407	83449	180-180	1.000000	79.2
trnH-GUG-rpl2_p5	trnH-GUG-rpl2	5	CGGATGTAGCCAAGTGGATC	CACAACCCCTTGGGGTTATC	20	20	55.0	55.0	58.1	58.2	0.2	35.0	0.281592	83407	83449	207-207	1.000000	78.1
rps15_p1	rps15	1	TGCTAATAACTGAACTAAGAAAAA	TCCTGATCAGTAAAATTCACAGA	24	23	25.0	34.8	52.7	55.3	0.0	21.1	15.000240	121281	121553	410-410	1.000000	40.0
rps15_p2	rps15	2	TCTAATGCTAATAACTGAACTAAGA	TCCTGATCAGTAAAATTCACAGA	25	23	28.0	34.8	53.6	55.3	0.0	21.1	15.082013	121281	121553	415-415	1.000000	40.0
rps15_p3	rps15	3	AATGCTAATAACTGAACTAAGAAA	TCCTGATCAGTAAAATTCACAGA	24	23	25.0	34.8	52.5	55.3	0.0	21.1	15.208045	121281	121553	412-412	1.000000	40.0
rps15_p4	rps15	4	ATGCTAATAACTGAACTAAGAAAA	TCCTGATCAGTAAAATTCACAGA	24	23	25.0	34.8	52.5	55.3	0.0	21.1	15.208045	121281	121553	411-411	1.000000	40.0
rps15_p5	rps15	5	TGCTAATAACTGAACTAAGAAAAA	TTCCTGATCAGTAAAATTCACAGA	24	24	25.0	33.3	52.7	56.1	0.0	21.1	15.287771	121281	121553	411-411	1.000000	40.0
rpl2-trnH-GUG_p1	rpl2-trnH-GUG	1	GTTATCCTGCGCTTGGAAGA	CGGATGTAGCCAAGTGGATC	20	20	50.0	55.0	58.0	58.1	22.4	0.2	0.153095	151524	151566	193-193	1.000000	79.2
rpl2-trnH-GUG_p2	rpl2-trnH-GUG	2	GTTATCCTGCGCTTGGAAGA	TGGATCAAGGCAGTGGATTG	20	20	50.0	50.0	58.0	57.9	22.4	0.0	0.157457	151524	151566	179-179	1.000000	79.2
rpl2-trnH-GUG_p3	rpl2-trnH-GUG	3	GTTATCCTGCGCTTGGAAGA	GGATCAAGGCAGTGGATTGT	20	20	50.0	50.0	58.0	57.9	22.4	0.0	0.158965	151524	151566	178-178	1.000000	79.2
rpl2-trnH-GUG_p4	rpl2-trnH-GUG	4	GTTATCCTGCGCTTGGAAGA	GTGGATCAAGGCAGTGGATT	20	20	50.0	50.0	58.0	57.9	22.4	0.0	0.158965	151524	151566	180-180	1.000000	79.2
rpl2-trnH-GUG_p5	rpl2-trnH-GUG	5	CACAACCCCTTGGGGTTATC	CGGATGTAGCCAAGTGGATC	20	20	55.0	55.0	58.2	58.1	35.0	0.2	0.281592	151524	151566	207-207	1.000000	78.1