primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	106	447	410-411	1.000000	85.1
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.208010	106	447	424-425	1.000000	85.0
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	106	447	423-424	1.000000	85.0
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	106	447	425-426	1.000000	85.0
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	AAAACAAAAGGGCGAACGAC	TTCCCTCTAGACCTAGCTGC	20	20	45.0	55.0	57.8	57.9	0.0	0.0	0.272521	106	447	474-474	0.500000	57.8
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCGATGGTATGGACGAATCC	TCAAAAGGGTGGGGGTTTTT	20	20	55.0	45.0	57.9	57.7	0.0	0.0	0.352360	4332	5131	951-953	1.000000	79.4
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CCGATGGTATGGACGAATCC	TTCAAAAGGGTGGGGGTTTT	20	20	55.0	45.0	57.9	57.7	0.0	0.0	0.352360	4332	5131	952-954	1.000000	79.4
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CCGATGGTATGGACGAATCC	TTTCAAAAGGGTGGGGGTTT	20	20	55.0	45.0	57.9	57.7	0.0	0.0	0.352360	4332	5131	953-955	1.000000	79.4
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCGATGGTATGGACGAATCC	TTTTCAAAAGGGTGGGGGTT	20	20	55.0	45.0	57.9	57.7	0.0	0.0	0.352360	4332	5131	954-956	1.000000	79.4
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	TCAAAAGGGTGGGGGTTTTT	20	20	50.0	45.0	58.2	57.7	20.3	0.0	0.440494	4332	5131	889-891	1.000000	78.8
rps2_p1	rps2	1	CAAGGTCTGCCCAGTTATTT	GCCAATCTACGGTAGAGCAG	20	20	45.0	55.0	55.6	57.9	0.0	21.3	2.525725	15923	16633	927-948	1.000000	62.0
rps2_p2	rps2	2	CAAGGTCTGCCCAGTTATTT	AGCCAATCTACGGTAGAGCA	20	20	45.0	50.0	55.6	58.2	0.0	21.3	2.607528	15923	16633	928-949	1.000000	61.4
rps2_p3	rps2	3	CAAGGTCTGCCCAGTTATTTT	GCCAATCTACGGTAGAGCAG	21	20	42.9	55.0	56.4	57.9	0.0	21.3	2.733806	15923	16633	927-948	1.000000	60.3
rps2_p4	rps2	4	CAAGGTCTGCCCAGTTATTTT	AGCCAATCTACGGTAGAGCA	21	20	42.9	50.0	56.4	58.2	0.0	21.3	2.815609	15923	16633	928-949	1.000000	59.7
rps2_p5	rps2	5	CAAGGTCTGCCCAGTTATTTTT	GCCAATCTACGGTAGAGCAG	22	20	40.9	55.0	57.1	57.9	0.0	21.3	3.013469	15923	16633	927-948	1.000000	58.1
trnG-GCC_p1	trnG-GCC	1	AGGAGAGTCTTTGGTCTGGG	ACTACACGATCACCGACAAC	20	20	55.0	50.0	58.3	57.7	0.0	0.0	0.692382	37850	37920	256-274	1.000000	75.1
trnG-GCC_p2	trnG-GCC	2	GGAGAGTCTTTGGTCTGGGA	ACTACACGATCACCGACAAC	20	20	55.0	50.0	58.4	57.7	0.0	0.0	0.697200	37850	37920	255-273	1.000000	75.0
trnG-GCC_p3	trnG-GCC	3	AGGAGAGTCTTTGGTCTGGG	GGGAGGCTATAGGAAAGGGA	20	20	55.0	55.0	58.3	57.6	0.0	1.0	0.767190	37850	37920	295-312	1.000000	74.7
trnG-GCC_p4	trnG-GCC	4	AGAGTCTTTGGTCTGGGACT	ACTACACGATCACCGACAAC	20	20	50.0	50.0	57.6	57.7	0.0	0.0	0.750250	37850	37920	253-271	1.000000	74.6
trnG-GCC_p5	trnG-GCC	5	GGAGAGTCTTTGGTCTGGGA	GGGAGGCTATAGGAAAGGGA	20	20	55.0	55.0	58.4	57.6	0.0	1.0	0.772009	37850	37920	294-311	1.000000	74.6
trnfM-CAU_p1	trnfM-CAU	1	TCTCTTTGCCAGGGAGAAGA	TTCATCGACGTTGTTTTGCG	20	20	50.0	45.0	58.0	58.0	5.8	2.0	0.041959	38108	38181	609-722	1.000000	84.5
trnfM-CAU_p2	trnfM-CAU	2	GTGCCCGTTGTATTTGCTTC	TTCATCGACGTTGTTTTGCG	20	20	50.0	45.0	58.0	58.0	0.0	2.0	0.034744	38108	38181	1098-1194	1.000000	84.4
trnfM-CAU_p3	trnfM-CAU	3	ATTAGCGTGCCCGTTGTATT	TTCATCGACGTTGTTTTGCG	20	20	45.0	45.0	58.0	58.0	0.0	2.0	0.044612	38108	38181	1104-1200	1.000000	84.3
trnfM-CAU_p4	trnfM-CAU	4	GTGCCCGTTGTATTTGCTTC	GCAAAGTTCCGTCATTGAGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.053708	38108	38181	1179-1275	1.000000	84.2
trnfM-CAU_p5	trnfM-CAU	5	GTGCCCGTTGTATTTGCTTC	CAATCCCCACCACGGAATAG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.055493	38108	38181	1132-1228	1.000000	84.2
atpB-rbcL_p1	atpB-rbcL	1	CTACATCTAGTACCGGGCCA	AACACCGGCTTTGAATCCAA	20	20	55.0	45.0	58.0	58.3	6.3	0.0	0.310373	56843	57634	915-943	1.000000	79.9
atpB-rbcL_p2	atpB-rbcL	2	GCGCAAAGGCTACATCTAGT	AACACCGGCTTTGAATCCAA	20	20	50.0	45.0	58.1	58.3	0.3	0.0	0.360169	56843	57634	924-952	1.000000	79.5
atpB-rbcL_p3	atpB-rbcL	3	GGAAACCCCAGAACCAGAAG	AACACCGGCTTTGAATCCAA	20	20	55.0	45.0	58.1	58.3	0.0	0.0	0.390437	56843	57634	854-882	1.000000	79.5
atpB-rbcL_p4	atpB-rbcL	4	CGATACGTCCCAGGTTGTTT	AACACCGGCTTTGAATCCAA	20	20	50.0	45.0	57.9	58.3	0.0	0.0	0.390444	56843	57634	885-913	1.000000	79.4
atpB-rbcL_p5	atpB-rbcL	5	TCTAGTACCGGGCCAATGAT	AACACCGGCTTTGAATCCAA	20	20	50.0	45.0	57.9	58.3	6.3	0.0	0.398604	56843	57634	910-938	1.000000	79.2
accD_p1	accD	1	TCCAATAACTATTCATCTATGGT	AGTTCACCAAACAAAATGCCT	23	21	30.4	38.1	52.4	56.8	0.0	0.0	10.761310	59532	61085	1678-1823	1.000000	45.1
accD_p2	accD	2	ATCCAATAACTATTCATCTATGGT	AGTTCACCAAACAAAATGCCT	24	21	29.2	38.1	53.1	56.8	0.0	0.0	11.125538	59532	61085	1679-1824	1.000000	45.1
accD_p3	accD	3	TCCAATAACTATTCATCTATGGT	AGTTCACCAAACAAAATGCC	23	20	30.4	40.0	52.4	55.2	0.0	0.0	11.321710	59532	61085	1678-1823	1.000000	45.1
accD_p4	accD	4	TCCAATAACTATTCATCTATGGT	GTTCACCAAACAAAATGCCT	23	20	30.4	40.0	52.4	55.2	0.0	0.0	11.321710	59532	61085	1677-1822	1.000000	45.1
accD_p5	accD	5	AATCCAATAACTATTCATCTATGGT	AGTTCACCAAACAAAATGCCT	25	21	28.0	38.1	53.9	56.8	0.0	0.0	11.338173	59532	61085	1680-1825	1.000000	45.1
psbE-petL_p1	psbE-petL	1	TGACAAATAACCAACCCGCA	AAGCCGCTAGTAGAAAACCA	20	20	45.0	45.0	58.0	56.9	0.0	0.0	1.160799	66599	67733	1282-1293	1.000000	71.9
psbE-petL_p2	psbE-petL	2	TGACAAATAACCAACCCGCA	AGCCGCTAGTAGAAAACCAA	20	20	45.0	45.0	58.0	56.9	0.0	0.0	1.160799	66599	67733	1281-1292	1.000000	71.9
psbE-petL_p3	psbE-petL	3	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAGAAAACCA	20	20	50.0	45.0	57.9	56.9	6.4	0.0	1.194794	66599	67733	1271-1282	1.000000	71.6
psbE-petL_p4	psbE-petL	4	CAACCCGCAATGAATAGGGA	AGCCGCTAGTAGAAAACCAA	20	20	50.0	45.0	57.9	56.9	6.4	0.0	1.194794	66599	67733	1270-1281	1.000000	71.6
psbE-petL_p5	psbE-petL	5	AGAACGTTCTCCTGTGCTTC	AGCCGCTAGTAGAAAACCAA	20	20	50.0	45.0	57.8	56.9	24.9	0.0	1.299803	66599	67733	1193-1204	1.000000	70.9
rps12-clpP1_p1	rps12-clpP1	1	AATTGAGATTTGCGCCAACG	CATTTAATCCCCAGGGTCCG	20	20	45.0	55.0	58.0	58.0	4.2	4.2	0.037490	72050	72160	891-925	1.000000	88.2
rps12-clpP1_p2	rps12-clpP1	2	AATTGAGATTTGCGCCAACG	ATGTCAGCAACAGAAGCACA	20	20	45.0	45.0	58.0	58.0	4.2	0.0	0.047265	72050	72160	476-502	1.000000	82.4
rps12-clpP1_p3	rps12-clpP1	3	AATTGAGATTTGCGCCAACG	CTCGCACGGGTTTATACACA	20	20	45.0	50.0	58.0	58.0	4.2	0.0	0.019698	72050	72160	551-551	0.500000	59.8
rps12-clpP1_p4	rps12-clpP1	4	TGCTACCAATGGAGAATCGC	CTCGCACGGGTTTATACACA	20	20	50.0	50.0	58.0	58.0	6.2	0.0	0.047393	72050	72160	582-582	0.500000	59.6
rps12-clpP1_p5	rps12-clpP1	5	TAGCTGACCCTCTTAGTCCG	CTCGCACGGGTTTATACACA	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.055184	72050	72160	802-802	0.500000	59.6
clpP1_p1	clpP1	1	ATTTGTGTGAAATCCGTCTT	GTTTCCACATCAAAGTAAAACT	20	22	35.0	31.8	53.4	53.2	0.0	0.0	11.432533	72161	74232	2140-2140	0.500000	20.0
clpP1_p2	clpP1	2	ATTTGTGTGAAATCCGTCTT	TCCACATCAAAGTAAAACTTAGTTCT	20	26	35.0	30.8	53.4	57.0	0.0	0.0	11.571207	72161	74232	2137-2137	0.500000	20.0
clpP1_p3	clpP1	3	ATTTGTGTGAAATCCGTCTT	GTTTCCACATCAAAGTAAAACTT	20	23	35.0	30.4	53.4	54.0	0.0	0.0	11.609336	72161	74232	2140-2140	0.500000	20.0
clpP1_p4	clpP1	4	ATTTGTGTGAAATCCGTCTT	TCCACATCAAAGTAAAACTTAGT	20	23	35.0	30.4	53.4	53.9	0.0	0.0	11.715788	72161	74232	2137-2137	0.500000	20.0
clpP1_p5	clpP1	5	ATTTGTGTGAAATCCGTCTT	TCCACATCAAAGTAAAACTTAGTTC	20	25	35.0	32.0	53.4	55.8	0.0	0.0	11.828480	72161	74232	2137-2137	0.500000	20.0
rps11-rpl36_p1	rps11-rpl36	1	CGTGACCCAATACGTCCATT	GCGGGGTCGAATTAGAGTAA	20	20	50.0	50.0	58.0	57.1	0.0	0.0	0.895585	81701	81811	225-225	1.000000	73.9
rps11-rpl36_p2	rps11-rpl36	2	GCACTCTTACGTGACCCAAT	GCGGGGTCGAATTAGAGTAA	20	20	50.0	50.0	57.9	57.1	0.0	0.0	0.971805	81701	81811	234-234	1.000000	73.3
rps11-rpl36_p3	rps11-rpl36	3	CGTGACCCAATACGTCCATT	GGCGGGGTCGAATTAGAGTA	20	20	50.0	55.0	58.0	59.0	0.0	0.0	0.983403	81701	81811	226-226	1.000000	73.2
rps11-rpl36_p4	rps11-rpl36	4	CCTTTGGGATTCTACGTGCA	GCGGGGTCGAATTAGAGTAA	20	20	50.0	50.0	57.9	57.1	0.0	0.0	0.984993	81701	81811	251-251	1.000000	73.1
rps11-rpl36_p5	rps11-rpl36	5	GCACTCTTACGTGACCCAAT	GGCGGGGTCGAATTAGAGTA	20	20	50.0	55.0	57.9	59.0	0.0	0.0	1.059623	81701	81811	235-235	1.000000	72.5
rps19-rpl2_p1	rps19-rpl2	1	GATGCTCGGGACCAAGTTAC	TTGATTCTTCGTCGCCGTAG	20	20	55.0	50.0	58.3	58.1	0.0	0.0	0.442705	86499	86522	164-211	1.000000	76.9
rps19-rpl2_p2	rps19-rpl2	2	GATGCTCGGGACCAAGTTAC	CACAACCCCTTGGGGTTATC	20	20	55.0	55.0	58.3	58.2	0.0	35.0	0.499520	86499	86522	231-278	1.000000	76.3
rps19-rpl2_p3	rps19-rpl2	3	AGATGCTCGGGACCAAGTTA	TTGATTCTTCGTCGCCGTAG	20	20	50.0	50.0	58.4	58.1	0.0	0.0	0.527352	86499	86522	165-212	1.000000	76.2
rps19-rpl2_p4	rps19-rpl2	4	TAGATGCTCGGGACCAAGTT	TTGATTCTTCGTCGCCGTAG	20	20	50.0	50.0	58.4	58.1	0.0	0.0	0.527352	86499	86522	166-213	1.000000	76.2
rps19-rpl2_p5	rps19-rpl2	5	GATGCTCGGGACCAAGTTAC	TTGGGGTTATCCTGCACTTG	20	20	55.0	50.0	58.3	57.8	0.0	0.0	0.561225	86499	86522	222-269	1.000000	75.8
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	CAGAGGATTAGAGCACGTGG	GCTCATATTGGTCGTGGGTT	20	20	55.0	50.0	57.8	58.0	18.9	0.0	0.254294	110208	110633	595-622	1.000000	78.6
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	CAGAGGATTAGAGCACGTGG	ATTGGTCGTGGGTTGAGAAC	20	20	55.0	50.0	57.8	58.1	18.9	0.0	0.323698	110208	110633	589-616	1.000000	78.1
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	20	20	55.0	50.0	57.8	58.2	18.9	18.9	0.372539	110208	110633	518-518	1.000000	77.8
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	CAGAGGATTAGAGCACGTGG	CGATTGGTCGTAGGTTCGAA	20	20	55.0	50.0	57.8	57.7	18.9	0.0	0.483095	110208	110633	522-522	1.000000	76.9
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	CAGAGGATTAGAGCACGTGG	TTCCTCAGTAGCTCAGTGGT	20	20	55.0	50.0	57.8	57.7	18.9	0.0	0.526686	110208	110633	561-561	1.000000	76.5
trnN-GUU-ycf1_p1	trnN-GUU-ycf1	1	CAATTGTGACCTCTCGGGAG	AATGGGTTCCGGTATTCTGC	20	20	55.0	50.0	58.0	57.9	4.9	0.0	0.068518	110706	111737	1230-1939	1.000000	84.7
trnN-GUU-ycf1_p2	trnN-GUU-ycf1	2	AGAATGATGGGGATAGCGGA	AATGGGTTCCGGTATTCTGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.082810	110706	111737	1502-2211	1.000000	84.5
trnN-GUU-ycf1_p3	trnN-GUU-ycf1	3	AGATAAACGCGGCTTCACAT	AATGGGTTCCGGTATTCTGC	20	20	45.0	50.0	58.0	57.9	12.5	0.0	0.076958	110706	111737	1728-2437	1.000000	84.4
trnN-GUU-ycf1_p4	trnN-GUU-ycf1	4	ATAAACGCGGCTTCACATCT	AATGGGTTCCGGTATTCTGC	20	20	45.0	50.0	58.0	57.9	12.5	0.0	0.076958	110706	111737	1726-2435	1.000000	84.4
trnN-GUU-ycf1_p5	trnN-GUU-ycf1	5	TTGTGACCTCTCGGGAGAAT	AATGGGTTCCGGTATTCTGC	20	20	50.0	50.0	58.1	57.9	15.7	0.0	0.114198	110706	111737	1227-1936	1.000000	84.4
ycf1_p1	ycf1	1	TCCCAGACTCAATATACAATTTCT	TGTTACTAACTTGGGTTTGTGA	24	22	33.3	36.4	55.3	55.5	0.0	6.3	11.156920	115108	115227	227-260	1.000000	46.6
ycf1_p2	ycf1	2	TCCCAGACTCAATATACAATTTCT	TTGTTACTAACTTGGGTTTGTGA	24	23	33.3	34.8	55.3	56.2	0.0	6.3	11.424079	115108	115227	228-261	1.000000	46.6
ycf1_p3	ycf1	3	TCCCAGACTCAATATACAATTTCTT	TGTTACTAACTTGGGTTTGTGA	25	22	32.0	36.4	56.0	55.5	0.0	6.3	11.466087	115108	115227	227-260	1.000000	46.6
ycf1_p4	ycf1	4	TTCCCAGACTCAATATACAATTTCT	TGTTACTAACTTGGGTTTGTGA	25	22	32.0	36.4	56.0	55.5	0.0	6.3	11.466087	115108	115227	228-261	1.000000	46.6
ycf1_p5	ycf1	5	TCCCAGACTCAATATACAATTTC	TGTTACTAACTTGGGTTTGTGA	23	22	34.8	36.4	53.9	55.5	0.0	6.3	11.583443	115108	115227	227-260	1.000000	46.6
ycf1-trnN-GUU_p1	ycf1-trnN-GUU	1	AATGGGTTCCGGTATTCTGC	CAATTGTGACCTCTCGGGAG	20	20	50.0	55.0	57.9	58.0	0.0	4.9	0.068518	136783	137814	1230-1939	1.000000	84.7
ycf1-trnN-GUU_p2	ycf1-trnN-GUU	2	AATGGGTTCCGGTATTCTGC	AGAATGATGGGGATAGCGGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.082810	136783	137814	1502-2211	1.000000	84.5
ycf1-trnN-GUU_p3	ycf1-trnN-GUU	3	AATGGGTTCCGGTATTCTGC	AGATAAACGCGGCTTCACAT	20	20	50.0	45.0	57.9	58.0	0.0	12.5	0.076958	136783	137814	1728-2437	1.000000	84.4
ycf1-trnN-GUU_p4	ycf1-trnN-GUU	4	AATGGGTTCCGGTATTCTGC	ATAAACGCGGCTTCACATCT	20	20	50.0	45.0	57.9	58.0	0.0	12.5	0.076958	136783	137814	1726-2435	1.000000	84.4
ycf1-trnN-GUU_p5	ycf1-trnN-GUU	5	AATGGGTTCCGGTATTCTGC	TTGTGACCTCTCGGGAGAAT	20	20	50.0	50.0	57.9	58.1	0.0	15.7	0.114198	136783	137814	1227-1936	1.000000	84.4