primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf2_p1	trnL-CAA-ycf2	1	ACAGCTTGGAGGTTCGAATC	TGAAGATATCTTTTCCGGTTGA	20	22	50.0	36.4	57.9	55.2	0.0	18.9	4.913798	4572	6149	1632-1912	1.000000	45.3
trnL-CAA-ycf2_p2	trnL-CAA-ycf2	2	ATTGAGTGCCTTGATGGTGG	TGAAGATATCTTTTCCGGTTGA	20	22	50.0	36.4	58.2	55.2	0.0	18.9	4.969825	4572	6149	1688-1968	1.000000	44.8
trnL-CAA-ycf2_p3	trnL-CAA-ycf2	3	CACAGCTTGGAGGTTCGAAT	TGAAGATATCTTTTCCGGTTGA	20	22	50.0	36.4	58.2	55.2	0.0	18.9	4.999680	4572	6149	1633-1913	1.000000	44.6
trnL-CAA-ycf2_p4	trnL-CAA-ycf2	4	AACGGTAAACACGCGAGATT	TGAAGATATCTTTTCCGGTTGA	20	22	45.0	36.4	58.2	55.2	15.9	18.9	5.026181	4572	6149	1668-1948	1.000000	44.6
trnL-CAA-ycf2_p5	trnL-CAA-ycf2	5	CGGAATGGAATTGAGTGCCT	TGAAGATATCTTTTCCGGTTGA	20	22	50.0	36.4	58.2	55.2	0.0	18.9	5.058014	4572	6149	1697-1977	1.000000	44.6
atpA_p1	atpA	1	CCACAGATTCCAGGAACCAC	ACCATGAAAAGCACGACTGA	20	20	55.0	45.0	58.2	57.7	3.2	0.0	0.428161	24979	26505	1644-1644	1.000000	76.6
atpA_p2	atpA	2	CCACAGATTCCAGGAACCAC	GGAACCATGAAAAGCACGAC	20	20	55.0	50.0	58.2	57.7	3.2	0.0	0.505424	24979	26505	1647-1647	1.000000	76.0
atpA_p3	atpA	3	CCACAGATTCCAGGAACCAC	TATCACATTGGCTTGCTCGG	20	20	55.0	50.0	58.2	58.3	3.2	0.0	0.506379	24979	26505	1665-1665	1.000000	76.0
atpA_p4	atpA	4	CCACAGATTCCAGGAACCAC	CTCGGAACCATGAAAAGCAC	20	20	55.0	50.0	58.2	57.4	3.2	0.0	0.792628	24979	26505	1650-1650	1.000000	73.7
atpA_p5	atpA	5	CACAGATTCCAGGAACCACA	ACCATGAAAAGCACGACTGA	20	20	50.0	45.0	57.4	57.7	3.2	0.0	0.821379	24979	26505	1643-1643	1.000000	73.5
atpF_p1	atpF	1	AACTACCGATCTCGTCAGGT	TTTGAGCGGGATTCCTGAAG	20	20	50.0	50.0	57.9	57.9	0.0	16.1	0.239633	26549	27839	1440-1440	1.000000	78.3
atpF_p2	atpF	2	ACTCCTCGATTTGTTTGCGA	TTTGAGCGGGATTCCTGAAG	20	20	45.0	50.0	57.8	57.9	0.0	16.1	0.277678	26549	27839	1464-1464	1.000000	78.0
atpF_p3	atpF	3	ACTACCGATCTCGTCAGGTC	TTTGAGCGGGATTCCTGAAG	20	20	55.0	50.0	58.3	57.9	0.0	16.1	0.447806	26549	27839	1439-1439	1.000000	76.6
atpF_p4	atpF	4	AACTACCGATCTCGTCAGGT	GAGCGGGATTCCTGAAGAAA	20	20	50.0	50.0	57.9	57.6	0.0	16.1	0.529025	26549	27839	1437-1437	1.000000	76.0
atpF_p5	atpF	5	ACTCCTCGATTTGTTTGCGA	GAGCGGGATTCCTGAAGAAA	20	20	45.0	50.0	57.8	57.6	0.0	16.1	0.567070	26549	27839	1461-1461	1.000000	75.7
rpoC1_p1	rpoC1	1	GCCATCGTGAAAACCTCGAA	GAAAAGCGACGGATGTTTGA	20	20	50.0	45.0	58.8	57.0	0.0	0.0	1.808169	34840	37690	2998-2998	1.000000	65.6
rpoC1_p2	rpoC1	2	GCCATCGTGAAAACCTCGAA	AGAAAAGCGACGGATGTTTG	20	20	50.0	45.0	58.8	57.0	0.0	0.0	1.816090	34840	37690	2999-2999	1.000000	65.5
rpoC1_p3	rpoC1	3	TCTGCCATCGTGAAAACCTC	AAAAGCGACGGATGTTTGAT	20	20	50.0	40.0	58.2	56.3	0.0	0.0	1.851247	34840	37690	3000-3000	1.000000	65.2
rpoC1_p4	rpoC1	4	ACCTCGAAAATTCTGTGAGCA	GAAAAGCGACGGATGTTTGA	21	20	42.9	45.0	57.9	57.0	0.0	0.0	2.087523	34840	37690	2986-2986	1.000000	63.3
rpoC1_p5	rpoC1	5	ACCTCGAAAATTCTGTGAGCA	AGAAAAGCGACGGATGTTTG	21	20	42.9	45.0	57.9	57.0	0.0	0.0	2.095444	34840	37690	2987-2987	1.000000	63.3
rpoC1-rpoB_p1	rpoC1-rpoB	1	GTTCGGGAGATGCTAACTGG	CCGGGAATTACGGTCGTTAG	20	20	55.0	55.0	58.1	58.2	0.0	11.8	0.214683	37691	37716	166-166	1.000000	78.8
rpoC1-rpoB_p2	rpoC1-rpoB	2	GTTCGGGAGATGCTAACTGG	GGTCGTTAGCTCCGAATCTG	20	20	55.0	55.0	58.1	58.2	0.0	9.7	0.217225	37691	37716	155-155	1.000000	78.8
rpoC1-rpoB_p3	rpoC1-rpoB	3	TCGGGAGATGCTAACTGGAT	CCGGGAATTACGGTCGTTAG	20	20	50.0	55.0	57.9	58.2	0.0	11.8	0.244112	37691	37716	164-164	1.000000	78.5
rpoC1-rpoB_p4	rpoC1-rpoB	4	TCGGGAGATGCTAACTGGAT	GGTCGTTAGCTCCGAATCTG	20	20	50.0	55.0	57.9	58.2	0.0	9.7	0.246655	37691	37716	153-153	1.000000	78.5
rpoC1-rpoB_p5	rpoC1-rpoB	5	TTCGGGAGATGCTAACTGGA	CCGGGAATTACGGTCGTTAG	20	20	50.0	55.0	58.1	58.2	0.0	11.8	0.295367	37691	37716	165-165	1.000000	78.1
rpoB_p1	rpoB	1	ACCACCTTATGCATGGTTTG	GATCGGGACAAATTGGTCGA	20	20	45.0	50.0	56.3	58.0	10.2	6.9	1.735594	39999	40118	262-262	1.000000	66.4
rpoB_p2	rpoB	2	ACCACCTTATGCATGGTTTG	TCAACAAAGGTGTGGATCGG	20	20	45.0	50.0	56.3	58.1	10.2	0.0	1.826176	39999	40118	276-276	1.000000	65.7
rpoB_p3	rpoB	3	ACCACCTTATGCATGGTTTG	TGGATCGGGACAAATTGGTC	20	20	45.0	50.0	56.3	57.9	10.2	4.2	1.839836	39999	40118	264-264	1.000000	65.6
rpoB_p4	rpoB	4	ACCACCTTATGCATGGTTTG	GGTGTGGATCGGGACAAATT	20	20	45.0	50.0	56.3	58.2	10.2	0.0	1.882864	39999	40118	268-268	1.000000	65.2
rpoB_p5	rpoB	5	ACCACCTTATGCATGGTTTG	GTGGATCGGGACAAATTGGT	20	20	45.0	50.0	56.3	58.2	10.2	0.0	1.882864	39999	40118	265-265	1.000000	65.2
petN-psbM_p1	petN-psbM	1	GGCTGCTCTGATGGTAGTCT	TCCCCTACGTACAAACAGCT	20	20	55.0	50.0	58.9	58.4	0.0	0.0	1.245014	41586	42213	730-732	1.000000	70.1
petN-psbM_p2	petN-psbM	2	GGCTGCTCTGATGGTAGTCT	ACCTACCGCTTCTTCACTCA	20	20	55.0	50.0	58.9	58.4	0.0	0.0	1.252119	41586	42213	750-752	1.000000	70.1
petN-psbM_p3	petN-psbM	3	GCTGCTCTGATGGTAGTCTT	TCCCCTACGTACAAACAGCT	20	20	50.0	50.0	57.0	58.4	0.0	0.0	1.341623	41586	42213	729-731	1.000000	69.4
petN-psbM_p4	petN-psbM	4	GCTGCTCTGATGGTAGTCTT	ACCTACCGCTTCTTCACTCA	20	20	50.0	50.0	57.0	58.4	0.0	0.0	1.348729	41586	42213	749-751	1.000000	69.3
petN-psbM_p5	petN-psbM	5	GGCTGCTCTGATGGTAGTCT	TCTTCACTCATCCCCTACGT	20	20	55.0	50.0	58.9	57.5	0.0	0.0	1.408050	41586	42213	740-742	1.000000	68.8
psbD-trnT-GGU_p1	psbD-trnT-GGU	1	GCCAGACCAACCCACAAATA	GTGGTAGAGTAACGCCATGG	20	20	50.0	55.0	58.1	58.1	0.0	7.6	0.146195	46944	47680	900-900	1.000000	79.0
psbD-trnT-GGU_p2	psbD-trnT-GGU	2	GCCAGACCAACCCACAAATA	AATTATTTCACCGGGAGCCC	20	20	50.0	50.0	58.1	57.9	0.0	6.3	0.149493	46944	47680	930-930	1.000000	79.0
psbD-trnT-GGU_p3	psbD-trnT-GGU	3	CGAAACGGTCTCTCCTTAGC	GTGGTAGAGTAACGCCATGG	20	20	55.0	55.0	58.1	58.1	0.0	7.6	0.153380	46944	47680	880-880	1.000000	79.0
psbD-trnT-GGU_p4	psbD-trnT-GGU	4	CGAAACGGTCTCTCCTTAGC	AATTATTTCACCGGGAGCCC	20	20	55.0	50.0	58.1	57.9	0.0	6.3	0.156678	46944	47680	910-910	1.000000	78.9
psbD-trnT-GGU_p5	psbD-trnT-GGU	5	GCCAGACCAACCCACAAATA	TCAGTGGTAGAGTAACGCCA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.168350	46944	47680	903-903	1.000000	78.8
petA-psbJ_p1	petA-psbJ	1	TAGCATCGGTTATTACGGCG	ACGGAGCATATTCGGGATTG	20	20	50.0	50.0	57.9	57.8	4.8	0.0	0.224520	77054	77694	762-762	1.000000	78.3
petA-psbJ_p2	petA-psbJ	2	TAGCATCGGTTATTACGGCG	GGAGCATATTCGGGATTGGG	20	20	50.0	55.0	57.9	58.2	4.8	0.0	0.228302	77054	77694	760-760	1.000000	78.3
petA-psbJ_p3	petA-psbJ	3	TAGCATCGGTTATTACGGCG	TCTACGGAGCATATTCGGGA	20	20	50.0	50.0	57.9	57.7	4.8	0.0	0.336226	77054	77694	765-765	1.000000	77.4
petA-psbJ_p4	petA-psbJ	4	TAGCATCGGTTATTACGGCG	TCGGGATTGGGATCATCTCT	20	20	50.0	50.0	57.9	57.6	4.8	0.0	0.460306	77054	77694	751-751	1.000000	76.4
petA-psbJ_p5	petA-psbJ	5	TTGCGTGTTCAAGGTCTTCT	ACGGAGCATATTCGGGATTG	20	20	45.0	50.0	57.7	57.8	0.0	0.0	0.495415	77054	77694	790-790	1.000000	76.1
rpl20-rps12_p1	rpl20-rps12	1	TCCGAGCTATACATCCACGT	TAGTGGGAGCGATTCCTCAT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.111048	81585	82229	719-719	1.000000	79.2
rpl20-rps12_p2	rpl20-rps12	2	TTACGACGTCTCCGAGCTAT	TAGTGGGAGCGATTCCTCAT	20	20	50.0	50.0	58.1	57.9	14.7	0.0	0.142717	81585	82229	729-729	1.000000	79.0
rpl20-rps12_p3	rpl20-rps12	3	TACTGTTACGACGTCTCCGA	TAGTGGGAGCGATTCCTCAT	20	20	50.0	50.0	57.9	57.9	14.7	0.0	0.166953	81585	82229	734-734	1.000000	78.8
rpl20-rps12_p4	rpl20-rps12	4	TCCGAGCTATACATCCACGT	GGAGCGATTCCTCATTCCTC	20	20	50.0	55.0	58.0	57.8	0.0	0.0	0.185263	81585	82229	714-714	1.000000	78.6
rpl20-rps12_p5	rpl20-rps12	5	TTACGACGTCTCCGAGCTAT	GGAGCGATTCCTCATTCCTC	20	20	50.0	55.0	58.1	57.8	14.7	0.0	0.216932	81585	82229	724-724	1.000000	78.4
rps8_p1	rps8	1	GGGAGGAATTTAGCGGTGAA	ATGCGTGAAGATTATGGGGG	20	20	50.0	50.0	57.6	57.7	0.0	0.0	0.690042	92912	93310	536-536	1.000000	74.6
rps8_p2	rps8	2	AGGGAGGAATTTAGCGGTGA	ATGCGTGAAGATTATGGGGG	20	20	50.0	50.0	58.4	57.7	0.0	0.0	0.696673	92912	93310	537-537	1.000000	74.6
rps8_p3	rps8	3	AAGGGAGGAATTTAGCGGTG	ATGCGTGAAGATTATGGGGG	20	20	50.0	50.0	57.6	57.7	0.0	0.0	0.696791	92912	93310	538-538	1.000000	74.6
rps8_p4	rps8	4	GGGAGGAATTTAGCGGTGAA	TGGGGGTAGGGTGATAGAGA	20	20	50.0	55.0	57.6	58.4	0.0	0.0	0.785462	92912	93310	522-522	1.000000	73.9
rps8_p5	rps8	5	AGGGAGGAATTTAGCGGTGA	TGGGGGTAGGGTGATAGAGA	20	20	50.0	55.0	58.4	58.4	0.0	0.0	0.792093	92912	93310	523-523	1.000000	73.8
rps8-rpl14_p1	rps8-rpl14	1	GAACCTTCGCATTCTTTCGC	ACCGAGAAGGAAATCCGAGA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.267872	93311	93655	519-525	1.000000	79.1
rps8-rpl14_p2	rps8-rpl14	2	CGAACCTTCGCATTCTTTCG	ACCGAGAAGGAAATCCGAGA	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.296401	93311	93655	520-526	1.000000	78.9
rps8-rpl14_p3	rps8-rpl14	3	CCTTCGCATTCTTTCGCATG	ACCGAGAAGGAAATCCGAGA	20	20	50.0	50.0	58.2	58.2	0.0	0.0	0.332498	93311	93655	516-522	1.000000	78.6
rps8-rpl14_p4	rps8-rpl14	4	GAACCTTCGCATTCTTTCGC	GTCACCAACCGAGAAGGAAA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.366367	93311	93655	526-532	1.000000	78.3
rps8-rpl14_p5	rps8-rpl14	5	CGAACCTTCGCATTCTTTCG	GTCACCAACCGAGAAGGAAA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.394896	93311	93655	527-533	1.000000	78.0
rpl16_p1	rpl16	1	GAACGTCGTGTGAGAACTGA	GGTTGTAGTAACACCATAGTCCC	20	23	50.0	47.8	57.9	58.2	0.0	0.0	3.352275	94198	95389	1329-1335	1.000000	53.7
rpl16_p2	rpl16	2	AACGTCGTGTGAGAACTGAG	GGTTGTAGTAACACCATAGTCCC	20	23	50.0	47.8	57.9	58.2	0.0	0.0	3.358043	94198	95389	1328-1334	1.000000	53.7
rpl16_p3	rpl16	3	GAACGTCGTGTGAGAACTGA	GTTGTAGTAACACCATAGTCCCA	20	23	50.0	43.5	57.9	57.6	0.0	0.0	3.525703	94198	95389	1328-1334	1.000000	52.3
rpl16_p4	rpl16	4	GAACGTCGTGTGAGAACTGA	TGGTTGTAGTAACACCATAGTCC	20	23	50.0	43.5	57.9	57.6	0.0	0.0	3.525703	94198	95389	1330-1336	1.000000	52.3
rpl16_p5	rpl16	5	AACGTCGTGTGAGAACTGAG	GTTGTAGTAACACCATAGTCCCA	20	23	50.0	43.5	57.9	57.6	0.0	0.0	3.531471	94198	95389	1327-1333	1.000000	52.3
rpl2_p1	rpl2	1	TCTCTCCTCTTGCGTTGAGA	TTGTTACACTCCGAACTGGC	20	20	50.0	50.0	58.1	58.1	2.6	0.0	0.215245	96918	98432	1709-1709	1.000000	78.3
rpl2_p2	rpl2	2	GGCTACGAAAGGACCCTTTT	TTGTTACACTCCGAACTGGC	20	20	50.0	50.0	57.8	58.1	14.6	0.0	0.322043	96918	98432	1663-1663	1.000000	77.5
rpl2_p3	rpl2	3	CTCTCTCCTCTTGCGTTGAG	TTGTTACACTCCGAACTGGC	20	20	55.0	50.0	57.7	58.1	0.0	0.0	0.402774	96918	98432	1710-1710	1.000000	76.8
rpl2_p4	rpl2	4	TCTCTCCTCTTGCGTTGAGA	ACACTCCGAACTGGCTATTC	20	20	50.0	50.0	58.1	57.3	2.6	0.0	0.763738	96918	98432	1704-1704	1.000000	73.9
rpl2_p5	rpl2	5	GGCTACGAAAGGACCCTTTT	ACACTCCGAACTGGCTATTC	20	20	50.0	50.0	57.8	57.3	14.6	0.0	0.870537	96918	98432	1658-1658	1.000000	73.1
ccsA_p1	ccsA	1	AAAGGTACGACCCTAGCCTT	TTGGTACCAGACGAACAATC	20	20	50.0	45.0	58.1	55.4	0.0	15.6	2.643551	112462	113415	1054-1054	1.000000	58.9
ccsA_p2	ccsA	2	AAAGGTACGACCCTAGCCTT	TTTGGTACCAGACGAACAATC	20	21	50.0	42.9	58.1	56.2	0.0	15.6	2.854699	112462	113415	1055-1055	1.000000	57.2
ccsA_p3	ccsA	3	CCTAGGTTCAGGTGTGCAAA	TTGGTACCAGACGAACAATC	20	20	50.0	45.0	57.7	55.4	0.0	15.6	2.862294	112462	113415	1090-1090	1.000000	57.2
ccsA_p4	ccsA	4	CCTAGGTTCAGGTGTGCAAA	TTTGGTACCAGACGAACAATC	20	21	50.0	42.9	57.7	56.2	0.0	15.6	3.073442	112462	113415	1091-1091	1.000000	55.5
ccsA_p5	ccsA	5	AAGGTACGACCCTAGCCTTC	TTGGTACCAGACGAACAATC	20	20	55.0	45.0	58.5	55.4	0.0	15.6	3.108988	112462	113415	1053-1053	1.000000	55.2
ndhD_p1	ndhD	1	CCAAATAATTCTCTATACATAAGTGCA	TCGGATCGATTGAAAAGACCC	27	21	29.6	47.6	55.9	58.1	0.0	0.0	10.216975	113553	115049	1688-1690	1.000000	40.1
ndhD_p2	ndhD	2	ACCAAATAATTCTCTATACATAAGTGC	TCGGATCGATTGAAAAGACCC	27	21	29.6	47.6	55.9	58.1	0.0	0.0	10.219067	113553	115049	1689-1691	1.000000	40.1
ndhD_p3	ndhD	3	CCAAATAATTCTCTATACATAAGTGCA	CGGATCGATTGAAAAGACCCA	27	21	29.6	47.6	55.9	58.4	0.0	0.0	10.490279	113553	115049	1687-1689	1.000000	40.1
ndhD_p4	ndhD	4	ACCAAATAATTCTCTATACATAAGTGC	CGGATCGATTGAAAAGACCCA	27	21	29.6	47.6	55.9	58.4	0.0	0.0	10.492370	113553	115049	1688-1690	1.000000	40.1
ndhD_p5	ndhD	5	TCTCTATACATAAGTGCAAAATAACT	TCGGATCGATTGAAAAGACCC	26	21	26.9	47.6	54.6	58.1	0.0	0.0	10.507664	113553	115049	1679-1681	1.000000	40.1
psaC-ndhE_p1	psaC-ndhE	1	GACAGGCTCTTACGCATTGA	TAGCTACACATCGCAATCGG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.144346	115410	115474	191-191	1.000000	79.3
psaC-ndhE_p2	psaC-ndhE	2	ACAGGCTCTTACGCATTGAG	TAGCTACACATCGCAATCGG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.149937	115410	115474	190-190	1.000000	79.2
psaC-ndhE_p3	psaC-ndhE	3	AGGCTCTTACGCATTGAGTG	TAGCTACACATCGCAATCGG	20	20	50.0	50.0	58.0	57.9	3.1	0.0	0.149937	115410	115474	188-188	1.000000	79.2
psaC-ndhE_p4	psaC-ndhE	4	CAGGCTCTTACGCATTGAGT	TAGCTACACATCGCAATCGG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.149937	115410	115474	189-189	1.000000	79.2
psaC-ndhE_p5	psaC-ndhE	5	GACAGGCTCTTACGCATTGA	CAGAAGCAGCCATCGGATTA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.255304	115410	115474	221-221	1.000000	78.3