primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.465225	78	465	506-506	1.000000	76.3
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.469587	78	465	520-520	1.000000	76.2
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	78	465	519-519	1.000000	76.2
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	78	465	521-521	1.000000	76.2
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	ACCGTGCTAACCTTGGTATG	20	20	55.0	50.0	58.1	57.6	0.0	0.0	0.525510	78	465	507-507	1.000000	75.8
psbA_p1	psbA	1	GAGCAATAACGCCCTCTTGA	TTGAAAATTCGTGCGCTTGG	20	20	50.0	45.0	58.0	58.2	0.0	9.7	0.259325	466	1527	1203-1203	1.000000	77.9
psbA_p2	psbA	2	AAAAGGAGCAATAACGCCCT	TTGAAAATTCGTGCGCTTGG	20	20	45.0	45.0	57.8	58.2	0.0	9.7	0.451091	466	1527	1208-1208	1.000000	76.4
psbA_p3	psbA	3	GAGCAATAACGCCCTCTTGA	ATATTGGTTGACACGGGCAT	20	20	50.0	45.0	58.0	57.6	0.0	0.0	0.459548	466	1527	1268-1268	1.000000	76.3
psbA_p4	psbA	4	GAGCAATAACGCCCTCTTGA	ATTGGTTGACACGGGCATAT	20	20	50.0	45.0	58.0	57.6	0.0	0.0	0.459548	466	1527	1266-1266	1.000000	76.3
psbA_p5	psbA	5	AAAGGAGCAATAACGCCCTC	TTGAAAATTCGTGCGCTTGG	20	20	50.0	45.0	58.2	58.2	0.0	9.7	0.483712	466	1527	1207-1207	1.000000	76.1
matK_p1	matK	1	ACATACTACATGAACATTTCAGA	ACCCCCAAAATCCTCTACCT	23	20	30.4	50.0	53.5	57.6	0.0	0.0	7.867308	2057	3577	1681-1681	1.000000	40.0
matK_p2	matK	2	ACATACTACATGAACATTTCAGAA	ACCCCCAAAATCCTCTACCT	24	20	29.2	50.0	54.3	57.6	0.0	0.0	8.080774	2057	3577	1681-1681	1.000000	40.0
matK_p3	matK	3	ACATACTACATGAACATTTCAGAAT	ACCCCCAAAATCCTCTACCT	25	20	28.0	50.0	54.8	57.6	0.0	0.0	8.549497	2057	3577	1681-1681	1.000000	40.0
matK_p4	matK	4	ACATACTACATGAACATTTCAGA	AACCCCCAAAATCCTCTACC	23	20	30.4	50.0	53.5	56.8	0.0	0.0	8.707648	2057	3577	1682-1682	1.000000	40.0
matK_p5	matK	5	CATACTACATGAACATTTCAGAA	ACCCCCAAAATCCTCTACCT	23	20	30.4	50.0	52.6	57.6	0.0	0.0	8.786814	2057	3577	1680-1680	1.000000	40.0
matK-trnK-UUU_p1	matK-trnK-UUU	1	ACCATGATCATGAGCAAGTACA	AACGGTAGAGTACTCGGCTT	22	20	40.9	50.0	57.5	58.2	32.7	17.1	2.660914	3578	4312	867-867	1.000000	58.7
matK-trnK-UUU_p2	matK-trnK-UUU	2	ACCATGATCATGAGCAAGTACA	ACGGTAGAGTACTCGGCTTT	22	20	40.9	50.0	57.5	58.2	32.7	17.1	2.660914	3578	4312	866-866	1.000000	58.7
matK-trnK-UUU_p3	matK-trnK-UUU	3	ACCATGATCATGAGCAAGTACA	TTGGGTTGCTAACTCAACGG	22	20	40.9	50.0	57.5	58.4	32.7	9.4	2.892471	3578	4312	882-882	1.000000	56.9
matK-trnK-UUU_p4	matK-trnK-UUU	4	ACCATGATCATGAGCAAGTACA	GGGTTGCTAACTCAACGGTA	22	20	40.9	50.0	57.5	57.5	32.7	0.0	2.953251	3578	4312	880-880	1.000000	56.4
matK-trnK-UUU_p5	matK-trnK-UUU	5	AACCATGATCATGAGCAAGT	ACGGTAGAGTACTCGGCTTT	20	20	40.0	50.0	55.0	58.2	32.7	17.1	3.163567	3578	4312	867-867	1.000000	54.7
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCGATGGTATGGACGAATCC	TCCTTGAAAAAGGGGCTCAG	20	20	55.0	50.0	57.9	57.7	0.0	4.7	0.373114	4350	5145	992-993	1.000000	77.0
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CGATGGTATGGACGAATCCC	TCCTTGAAAAAGGGGCTCAG	20	20	55.0	50.0	57.9	57.7	0.0	4.7	0.373114	4350	5145	991-992	1.000000	77.0
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAAGCCGAGTACTCTACCGT	TCCTTGAAAAAGGGGCTCAG	20	20	50.0	50.0	58.2	57.7	20.3	4.7	0.461248	4350	5145	931-932	1.000000	76.3
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	TCCTTGAAAAAGGGGCTCAG	20	20	50.0	50.0	58.2	57.7	20.3	4.7	0.461248	4350	5145	930-931	1.000000	76.3
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCAGTCGTGGTCTTCCAAAC	TCCTTGAAAAAGGGGCTCAG	20	20	50.0	50.0	57.8	57.7	0.0	4.7	0.527128	4350	5145	1016-1017	1.000000	75.8
rps16_p1	rps16	1	ACGAATAGAGCAAGAAAAGAAGG	TCTCTGTACCGACTGAACCA	23	20	39.1	50.0	56.9	57.7	0.0	0.0	4.403459	5146	6260	1301-1301	1.000000	44.8
rps16_p2	rps16	2	ACGAATAGAGCAAGAAAAGAAGGA	TCTCTGTACCGACTGAACCA	24	20	37.5	50.0	58.2	57.7	0.0	0.0	4.486645	5146	6260	1301-1301	1.000000	44.1
rps16_p3	rps16	3	CGAATAGAGCAAGAAAAGAAGGA	TCTCTGTACCGACTGAACCA	23	20	39.1	50.0	56.6	57.7	0.0	0.0	4.654102	5146	6260	1300-1300	1.000000	42.8
rps16_p4	rps16	4	ACGAATAGAGCAAGAAAAGAAGG	GTCTCTGTACCGACTGAACC	23	20	39.1	55.0	56.9	57.4	0.0	0.0	4.755119	5146	6260	1302-1302	1.000000	42.0
rps16_p5	rps16	5	ACGAATAGAGCAAGAAAAGAAGGA	GTCTCTGTACCGACTGAACC	24	20	37.5	55.0	58.2	57.4	0.0	0.0	4.838305	5146	6260	1302-1302	1.000000	41.3
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6261	7200	1013-1013	1.000000	78.3
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	7.8	16.9	0.445953	6261	7200	1028-1028	1.000000	76.4
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	6261	7200	1027-1027	1.000000	76.4
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CCAAGAGCACCTTCATTCCT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.5	58.1	0.0	16.9	0.579996	6261	7200	1083-1083	1.000000	75.4
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6261	7200	1017-1017	1.000000	73.5
trnS-GCU-trnC-GCA_p1	trnS-GCU-trnC-GCA	1	ACGGAAAGAGAGGGATTCGA	GTTTGTTAATGAGGCGGCAC	20	20	50.0	50.0	58.2	58.0	0.0	13.1	0.174160	8551	9316	937-937	1.000000	78.6
trnS-GCU-trnC-GCA_p2	trnS-GCU-trnC-GCA	2	ACGGAAAGAGAGGGATTCGA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.233476	8551	9316	917-917	1.000000	78.1
trnS-GCU-trnC-GCA_p3	trnS-GCU-trnC-GCA	3	ACGGAAAGAGAGGGATTCGA	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.2	58.4	0.0	14.6	0.506839	8551	9316	899-899	1.000000	75.9
trnS-GCU-trnC-GCA_p4	trnS-GCU-trnC-GCA	4	CTTTCGTCCACTCAGCCATC	GTTTGTTAATGAGGCGGCAC	20	20	55.0	50.0	58.6	58.0	0.0	13.1	0.659068	8551	9316	873-873	1.000000	74.7
trnS-GCU-trnC-GCA_p5	trnS-GCU-trnC-GCA	5	AACGGAAAGAGAGGGATTCG	GTTTGTTAATGAGGCGGCAC	20	20	50.0	50.0	57.3	58.0	0.0	13.1	0.697607	8551	9316	938-938	1.000000	74.4
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	9388	10218	887-887	1.000000	78.1
trnC-GCA-petN_p2	trnC-GCA-petN	2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	9388	10218	905-905	1.000000	75.9
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	20	20	50.0	50.0	58.1	57.1	0.0	0.0	0.974912	9388	10218	886-886	1.000000	72.2
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	20	20	50.0	50.0	58.1	58.9	0.0	0.0	0.984078	9388	10218	895-895	1.000000	72.1
trnC-GCA-petN_p5	trnC-GCA-petN	5	ATTGTGTATCGTTTTGGCGG	AGCCCAAGCGAGACTTACTA	20	20	45.0	50.0	57.1	58.2	0.0	0.0	1.063167	9388	10218	944-944	1.000000	71.5
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AAATGAACAGAGCAGTAGCA	TAGATCCCGTCTGATTCCCC	20	20	40.0	55.0	55.0	58.0	0.0	0.0	3.030237	10927	11601	815-816	1.000000	55.9
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AATGAACAGAGCAGTAGCAA	TAGATCCCGTCTGATTCCCC	20	20	40.0	55.0	55.0	58.0	0.0	0.0	3.030237	10927	11601	814-815	1.000000	55.9
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AAATGAACAGAGCAGTAGCA	TCTGATTCCCCTGGGATTGT	20	20	40.0	50.0	55.0	58.0	0.0	16.2	3.033493	10927	11601	806-807	1.000000	55.8
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AATGAACAGAGCAGTAGCAA	TCTGATTCCCCTGGGATTGT	20	20	40.0	50.0	55.0	58.0	0.0	16.2	3.033493	10927	11601	805-806	1.000000	55.8
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AAATGAACAGAGCAGTAGCAA	TAGATCCCGTCTGATTCCCC	21	20	38.1	55.0	55.8	58.0	0.0	0.0	3.210810	10927	11601	815-816	1.000000	54.4
trnE-UUC-rpoB_p1	trnE-UUC-rpoB	1	AACCCTCGTATTCTCCGTGA	AGTTCTTCTGTCAAACCCTGA	20	21	50.0	42.9	58.2	56.8	0.0	0.0	2.370754	12032	12942	1046-1046	1.000000	61.0
trnE-UUC-rpoB_p2	trnE-UUC-rpoB	2	ACCCTCGTATTCTCCGTGAA	AGTTCTTCTGTCAAACCCTGA	20	21	50.0	42.9	58.2	56.8	0.0	0.0	2.370754	12032	12942	1045-1045	1.000000	61.0
trnE-UUC-rpoB_p3	trnE-UUC-rpoB	3	TGAACCCTCGTATTCTCCGT	AGTTCTTCTGTCAAACCCTGA	20	21	50.0	42.9	58.2	56.8	0.0	0.0	2.370754	12032	12942	1048-1048	1.000000	61.0
trnE-UUC-rpoB_p4	trnE-UUC-rpoB	4	GAACCCTCGTATTCTCCGTG	AGTTCTTCTGTCAAACCCTGA	20	21	55.0	42.9	57.8	56.8	0.0	0.0	2.419429	12032	12942	1047-1047	1.000000	60.6
trnE-UUC-rpoB_p5	trnE-UUC-rpoB	5	ACCCTCGTATTCTCCGTGAA	TCAAACCCTGATCAATGAACCT	20	22	50.0	40.9	58.2	57.6	0.0	0.0	2.543658	12032	12942	1035-1035	1.000000	59.7