primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-rps16_p1	trnK-rps16	1	CGAATTCCTCGCTTCATCCA	AAAAGGGTATGTTGCTGCCA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.068268	4407	5290	1602-1713	1.000000	86.5
trnK-rps16_p2	trnK-rps16	2	CGAATTCCTCGCTTCATCCA	TCTATCCCAATGAGCCGTCT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.148130	4407	5290	1133-1229	1.000000	86.2
trnK-rps16_p3	trnK-rps16	3	CGAATTCCTCGCTTCATCCA	GCCGTACGAGGAGAAAACTT	20	20	50.0	50.0	58.1	57.9	0.0	18.8	0.136005	4407	5290	1189-1285	1.000000	86.1
trnK-rps16_p4	trnK-rps16	4	TTCCGTTCCACGAATTCGAA	AAAAGGGTATGTTGCTGCCA	20	20	45.0	45.0	57.8	58.0	33.1	0.0	0.160123	4407	5290	1751-1862	1.000000	85.6
trnK-rps16_p5	trnK-rps16	5	CGAATTCCTCGCTTCATCCA	CCCAGAACAAGGAAAGACCC	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.148968	4407	5290	1587-1589	0.086957	42.3
rps16-trnQ(UUG)_p1	rps16-trnQ(UUG)	1	CGGATCTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.1	58.1	0.0	16.9	0.161467	6435	7691	1336-1759	1.000000	82.1
rps16-trnQ(UUG)_p2	rps16-trnQ(UUG)	2	ATCTGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	0.0	16.9	0.162155	6435	7691	1333-1756	1.000000	82.1
rps16-trnQ(UUG)_p3	rps16-trnQ(UUG)	3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6435	7691	1322-1745	1.000000	81.7
rps16-trnQ(UUG)_p4	rps16-trnQ(UUG)	4	CCACAACGGATCTGTCCTTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.3	58.1	7.9	16.9	0.349248	6435	7691	1342-1765	1.000000	80.6
rps16-trnQ(UUG)_p5	rps16-trnQ(UUG)	5	GGATCTGTCCTTCAAGTCGC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	0.0	16.9	0.429912	6435	7691	1335-1758	1.000000	80.0
psbI-trnS(GCU)_p1	psbI-trnS(GCU)	1	CGTAATCCTGGACGTGAAGA	TGGACTAAAGCGTCGGATTG	20	20	50.0	50.0	57.1	58.0	0.0	0.0	0.938078	8800	8925	211-223	1.000000	84.5
psbI-trnS(GCU)_p2	psbI-trnS(GCU)	2	CGTAATCCTGGACGTGAAGA	GTGGACTAAAGCGTCGGATT	20	20	50.0	50.0	57.1	58.0	0.0	0.0	0.939466	8800	8925	212-224	1.000000	84.5
psbI-trnS(GCU)_p3	psbI-trnS(GCU)	3	CGTAATCCTGGACGTGAAGA	ATTGGGAGAGATGGCTGAGT	20	20	50.0	50.0	57.1	58.1	0.0	0.0	1.042943	8800	8925	230-242	1.000000	83.7
psbI-trnS(GCU)_p4	psbI-trnS(GCU)	4	CGTAATCCTGGACGTGAAGAA	TGGACTAAAGCGTCGGATTG	21	20	47.6	50.0	57.8	58.0	0.0	0.0	1.230929	8800	8925	211-223	1.000000	82.2
psbI-trnS(GCU)_p5	psbI-trnS(GCU)	5	CGTAATCCTGGACGTGAAGAA	GTGGACTAAAGCGTCGGATT	21	20	47.6	50.0	57.8	58.0	0.0	0.0	1.232316	8800	8925	212-224	1.000000	82.1
trnS(GCU)-trnG(GCC)_p1	trnS(GCU)-trnG(GCC)	1	CAATCCGACGCTTTAGTCCA	AGACCGAAAGACCCTTTAACT	20	21	50.0	42.9	58.0	56.6	0.0	0.0	2.425660	9014	9720	798-831	1.000000	68.3
trnS(GCU)-trnG(GCC)_p2	trnS(GCU)-trnG(GCC)	2	AATCCGACGCTTTAGTCCAC	AGACCGAAAGACCCTTTAACT	20	21	50.0	42.9	58.0	56.6	0.0	0.0	2.427048	9014	9720	797-830	1.000000	68.3
trnS(GCU)-trnG(GCC)_p3	trnS(GCU)-trnG(GCC)	3	ATCAACGGAACCGGAAAGAG	AGACCGAAAGACCCTTTAACT	20	21	50.0	42.9	57.9	56.6	9.5	0.0	2.515840	9014	9720	862-895	1.000000	67.0
trnS(GCU)-trnG(GCC)_p4	trnS(GCU)-trnG(GCC)	4	CATCAACGGAACCGGAAAGA	AGACCGAAAGACCCTTTAACT	20	21	50.0	42.9	58.2	56.6	9.5	0.0	2.617743	9014	9720	863-896	1.000000	66.2
trnS(GCU)-trnG(GCC)_p5	trnS(GCU)-trnG(GCC)	5	TCATCAACGGAACCGGAAAG	AGACCGAAAGACCCTTTAACT	20	21	50.0	42.9	58.2	56.6	9.5	0.0	2.617743	9014	9720	864-897	1.000000	66.2
atpA-atpF_p1	atpA-atpF	1	ATGCCATCGCCTACTTGAAG	TGTTGGGAGCGATGAAAGAA	20	20	50.0	45.0	58.0	57.4	0.0	0.0	0.600656	12351	12412	210-229	1.000000	77.4
atpA-atpF_p2	atpA-atpF	2	ATGCCATCGCCTACTTGAAG	ATGTTGGGAGCGATGAAAGA	20	20	50.0	45.0	58.0	57.2	0.0	0.0	0.828431	12351	12412	211-230	1.000000	75.6
atpA-atpF_p3	atpA-atpF	3	ATGCCATCGCCTACTTGAAG	CATGTTGGGAGCGATGAAAG	20	20	50.0	50.0	58.0	57.2	0.0	0.0	0.879345	12351	12412	212-231	1.000000	75.2
atpA-atpF_p4	atpA-atpF	4	CAATGCCATCGCCTACTTGA	TGTTGGGAGCGATGAAAGAA	20	20	50.0	45.0	58.3	57.4	0.0	0.0	0.892462	12351	12412	212-231	1.000000	75.1
atpA-atpF_p5	atpA-atpF	5	ATGCCATCGCCTACTTGAAG	ATATTGGCATGTTGGGAGCG	20	20	50.0	50.0	58.0	58.7	0.0	0.0	0.719484	12351	12412	219-228	0.956522	74.0
atpH-atpI_p1	atpH-atpI	1	AACAGAAGCGGCAGAAATCA	TCCCTGTCATGTTCCTTGGA	20	20	45.0	50.0	58.1	58.3	1.4	0.0	0.362752	14276	15357	1203-1216	1.000000	80.5
atpH-atpI_p2	atpH-atpI	2	TACCCTCTACAGCTTGACCC	TCCCTGTCATGTTCCTTGGA	20	20	55.0	50.0	58.1	58.3	0.0	0.0	0.396990	14276	15357	1288-1301	1.000000	80.0
atpH-atpI_p3	atpH-atpI	3	GCGATACCCTCTACAGCTTG	TCCCTGTCATGTTCCTTGGA	20	20	55.0	50.0	57.9	58.3	0.0	0.0	0.400507	14276	15357	1292-1305	1.000000	80.0
atpH-atpI_p4	atpH-atpI	4	CAGCAGCAATAACAGAAGCG	TCCCTGTCATGTTCCTTGGA	20	20	50.0	50.0	57.8	58.3	0.0	0.0	0.428635	14276	15357	1213-1226	1.000000	79.9
atpH-atpI_p5	atpH-atpI	5	GCCAATCCAGCAGCAATAAC	TCCCTGTCATGTTCCTTGGA	20	20	50.0	50.0	57.8	58.3	0.0	0.0	0.473359	14276	15357	1220-1233	1.000000	79.6
rps2-rpoC2_p1	rps2-rpoC2	1	TGCCTCCATCATCTCTTCCA	TTCGAGGGGGAAATGAGAGA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.364021	17034	17241	369-381	1.000000	82.7
rps2-rpoC2_p2	rps2-rpoC2	2	AACTCCTGCCTCCATCATCT	TTCGAGGGGGAAATGAGAGA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.357147	17034	17241	375-387	1.000000	82.6
rps2-rpoC2_p3	rps2-rpoC2	3	TCCTGCCTCCATCATCTCTT	TTCGAGGGGGAAATGAGAGA	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.434938	17034	17241	372-384	1.000000	82.0
rps2-rpoC2_p4	rps2-rpoC2	4	GAACTCCTGCCTCCATCATC	TTCGAGGGGGAAATGAGAGA	20	20	55.0	50.0	57.7	57.8	0.0	0.0	0.500676	17034	17241	376-388	1.000000	81.5
rps2-rpoC2_p5	rps2-rpoC2	5	CTCCTGCCTCCATCATCTCT	TTCGAGGGGGAAATGAGAGA	20	20	55.0	50.0	58.3	57.8	0.0	0.0	0.528170	17034	17241	373-385	1.000000	81.3
psbM-trnD(GUC)_p1	psbM-trnD(GUC)	1	AGAATGAATAGTGCAGTCGC	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	55.9	58.5	0.0	5.4	2.530985	30884	31530	636-759	1.000000	67.6
psbM-trnD(GUC)_p2	psbM-trnD(GUC)	2	AGAATGAATAGTGCAGTCGC	GTTCAATTGGTCAGAGCACC	20	20	45.0	50.0	55.9	57.0	0.0	5.4	3.051139	30884	31530	637-760	1.000000	63.4
psbM-trnD(GUC)_p3	psbM-trnD(GUC)	3	AGTAGAAAAGCGGTAGGAACT	TTCAATTGGTCAGAGCACCG	21	20	42.9	50.0	56.4	58.5	0.0	5.4	3.088455	30884	31530	657-780	1.000000	63.1
psbM-trnD(GUC)_p4	psbM-trnD(GUC)	4	AGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	55.4	58.5	0.0	5.4	3.107053	30884	31530	649-772	1.000000	62.9
psbM-trnD(GUC)_p5	psbM-trnD(GUC)	5	AGAATGAATAGTGCAGTCGC	TCAATTGGTCAGAGCACCG	20	19	45.0	52.6	55.9	57.8	0.0	5.4	3.285388	30884	31530	635-758	1.000000	61.6
ycf3-trnS(GGA)_p1	ycf3-trnS(GGA)	1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	21	20	47.6	50.0	58.0	58.1	8.2	0.0	1.134847	45737	46532	878-899	1.000000	75.5
ycf3-trnS(GGA)_p2	ycf3-trnS(GGA)	2	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	21	20	47.6	50.0	58.0	58.2	8.2	0.0	1.191833	45737	46532	899-920	1.000000	74.9
ycf3-trnS(GGA)_p3	ycf3-trnS(GGA)	3	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	21	20	47.6	45.0	58.0	57.8	8.2	5.6	1.233148	45737	46532	849-870	1.000000	74.9
ycf3-trnS(GGA)_p4	ycf3-trnS(GGA)	4	CATTTATCCGCGATCTAGGCA	ACCCTCGGTAAACAAAAGCC	21	20	47.6	50.0	58.0	58.4	8.2	0.0	1.427808	45737	46532	879-900	1.000000	73.2
ycf3-trnS(GGA)_p5	ycf3-trnS(GGA)	5	TTTATCCGCGATCTAGGCAT	CCCTCGGTAAACAAAAGCCT	20	20	45.0	50.0	56.6	58.1	8.2	0.0	1.514239	45737	46532	876-897	1.000000	72.5
ndhC-trnM(CAU)_p1	ndhC-trnM(CAU)	1	GGGGCTAAAATCCCGGAAAT	CCCGCCGTATGAAAGCAATA	20	20	50.0	50.0	57.9	58.4	0.7	0.0	0.493466	51942	53888	2068-2092	1.000000	80.1
ndhC-trnM(CAU)_p2	ndhC-trnM(CAU)	2	GGGGCTAAAATCCCGGAAAT	AGGTACCATTGACTCCGGTA	20	20	50.0	50.0	57.9	57.5	0.7	15.9	0.619773	51942	53888	2129-2153	1.000000	79.0
ndhC-trnM(CAU)_p3	ndhC-trnM(CAU)	3	GGGGCTAAAATCCCGGAAAT	TAGGTACCATTGACTCCGGT	20	20	50.0	50.0	57.9	57.5	0.7	15.9	0.619773	51942	53888	2130-2154	1.000000	79.0
ndhC-trnM(CAU)_p4	ndhC-trnM(CAU)	4	GGGGCTAAAATCCCGGAAAT	CCGCCGTATGAAAGCAATAC	20	20	50.0	50.0	57.9	57.3	0.7	0.0	0.745419	51942	53888	2067-2091	1.000000	78.1
ndhC-trnM(CAU)_p5	ndhC-trnM(CAU)	5	TTCTCCGGCCCTTTCCTAAT	CCCGCCGTATGAAAGCAATA	20	20	50.0	50.0	58.4	58.4	0.0	0.0	0.819557	51942	53888	2089-2113	1.000000	77.4
atpB-rbcL_p1	atpB-rbcL	1	ATACGGCCCAGGTTTTGTTT	AACACCCGCTTTGAATCCAA	20	20	45.0	45.0	58.0	58.3	0.0	0.0	0.305431	56062	56846	903-911	1.000000	79.9
atpB-rbcL_p2	atpB-rbcL	2	GGAAACCCCAGAACCAGAAG	AACACCCGCTTTGAATCCAA	20	20	55.0	45.0	58.1	58.3	0.0	0.0	0.390437	56062	56846	874-882	1.000000	79.3
atpB-rbcL_p3	atpB-rbcL	3	GGCGGAAAGGCTACATCTAG	AACACCCGCTTTGAATCCAA	20	20	55.0	45.0	57.8	58.3	0.0	0.0	0.457837	56062	56846	945-953	1.000000	78.7
atpB-rbcL_p4	atpB-rbcL	4	TACGGCCCAGGTTTTGTTTT	AACACCCGCTTTGAATCCAA	20	20	45.0	45.0	58.2	58.3	0.0	0.0	0.523554	56062	56846	902-910	1.000000	78.2
atpB-rbcL_p5	atpB-rbcL	5	GGACCGATGATTTGGACGAT	AACACCCGCTTTGAATCCAA	20	20	50.0	45.0	57.8	58.3	0.0	0.0	0.541987	56062	56846	921-929	1.000000	78.1
ycf4-cemA_p1	ycf4-cemA	1	CATTCCCTTGACCCGTACTG	GAGAAATCCACCAGGGCAAA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.100820	62231	63103	711-1071	1.000000	85.8
ycf4-cemA_p2	ycf4-cemA	2	ATTCCCTTGACCCGTACTGA	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.129660	62231	63103	710-1070	1.000000	85.6
ycf4-cemA_p3	ycf4-cemA	3	TTCCCTTGACCCGTACTGAT	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.129660	62231	63103	709-1069	1.000000	85.6
ycf4-cemA_p4	ycf4-cemA	4	CTTGCGCGTACCAATTGAAG	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.1	58.1	8.4	0.0	0.194446	62231	63103	636-996	1.000000	85.2
ycf4-cemA_p5	ycf4-cemA	5	GAACAAAAAGCCGCCGAATT	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	58.2	58.1	0.0	0.0	0.298310	62231	63103	665-1025	1.000000	84.4
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.9	57.1	6.4	0.0	0.992787	66708	67873	1352-1375	1.000000	76.5
psbE-petL_p2	psbE-petL	2	ACTGACGAATAACCAACCCG	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.9	57.1	0.0	0.0	1.030402	66708	67873	1365-1388	1.000000	76.2
psbE-petL_p3	psbE-petL	3	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	24.9	0.0	1.097796	66708	67873	1275-1298	1.000000	75.9
psbE-petL_p4	psbE-petL	4	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	20	20	45.0	50.0	58.2	57.1	0.0	0.0	1.112474	66708	67873	1361-1384	1.000000	75.5
psbE-petL_p5	psbE-petL	5	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	0.0	0.0	1.128804	66708	67873	1360-1383	1.000000	75.4
ndhF_p1	ndhF	1	GCAGAATACCGTCACCCATT	GAGGTAAGATCCGGTGAATCG	20	21	50.0	52.4	58.0	58.0	0.0	0.0	1.052991	111637	113853	2628-2634	1.000000	74.0
ndhF_p2	ndhF	2	GAGATCCGCCGAAGAAGATC	GAGGTAAGATCCGGTGAATCG	20	21	55.0	52.4	57.9	58.0	0.0	0.0	1.073517	111637	113853	2492-2498	1.000000	73.9
ndhF_p3	ndhF	3	CAGGAACAAGAGAGATCCGC	GAGGTAAGATCCGGTGAATCG	20	21	55.0	52.4	58.1	58.0	0.0	0.0	1.081357	111637	113853	2503-2509	1.000000	73.8
ndhF_p4	ndhF	4	CGCCGAAGAAGATCCTTCTC	GAGGTAAGATCCGGTGAATCG	20	21	55.0	52.4	58.2	58.0	9.4	0.0	1.168063	111637	113853	2486-2487	0.956522	71.2
ndhF_p5	ndhF	5	GCCGAAGAAGATCCTTCTCC	CGCTGTCCCATATCCTTTCC	20	20	55.0	55.0	57.8	58.1	9.4	0.0	0.348521	111637	113853	2996-3000	0.478261	58.8
ndhF-rpl32_p1	ndhF-rpl32	1	ACAAAAGGTATGATCCACGCA	TGATGCAGAAGTACGTTTTT	21	20	42.9	35.0	57.9	53.5	0.0	0.0	5.602406	113854	114332	549-558	1.000000	46.1
ndhF-rpl32_p2	ndhF-rpl32	2	ACAAAAGGTATGATCCACGCA	TTGATGCAGAAGTACGTTTT	21	20	42.9	35.0	57.9	53.5	0.0	0.0	5.602406	113854	114332	550-559	1.000000	46.1
ndhF-rpl32_p3	ndhF-rpl32	3	ACAAAAGGTATGATCCACGCA	TTTGATGCAGAAGTACGTTT	21	20	42.9	35.0	57.9	53.5	0.0	0.0	5.602406	113854	114332	551-560	1.000000	46.0
ndhF-rpl32_p4	ndhF-rpl32	4	ACAAAAGGTATGATCCACGCA	TTTTGATGCAGAAGTACGTT	21	20	42.9	35.0	57.9	53.5	0.0	0.0	5.602406	113854	114332	552-561	1.000000	46.0
ndhF-rpl32_p5	ndhF-rpl32	5	ACAAAAGGTATGATCCACGCA	TTTTTGATGCAGAAGTACGT	21	20	42.9	35.0	57.9	53.5	0.0	0.0	5.602406	113854	114332	553-562	1.000000	46.0
rpl32-trnL(UAG)_p1	rpl32-trnL(UAG)	1	ACCGGGAATTCAAAAAGTTT	TCCTAAGAGCAGCGTGTCTA	20	20	35.0	50.0	53.9	57.9	6.3	0.0	4.271580	114492	115350	626-949	1.000000	53.4
rpl32-trnL(UAG)_p2	rpl32-trnL(UAG)	2	ACCGGGAATTCAAAAAGTTT	AGCAGCGTGTCTACCAATTT	20	20	35.0	45.0	53.9	57.8	6.3	0.0	4.332307	114492	115350	619-942	1.000000	53.0
rpl32-trnL(UAG)_p3	rpl32-trnL(UAG)	3	ACCGGGAATTCAAAAAGTTT	CCTAAGAGCAGCGTGTCTAC	20	20	35.0	55.0	53.9	57.8	6.3	0.0	4.343598	114492	115350	625-948	1.000000	52.8
rpl32-trnL(UAG)_p4	rpl32-trnL(UAG)	4	ACCGGGAATTCAAAAAGTTT	CTAAGAGCAGCGTGTCTACC	20	20	35.0	55.0	53.9	57.8	6.3	0.0	4.343598	114492	115350	624-947	1.000000	52.8
rpl32-trnL(UAG)_p5	rpl32-trnL(UAG)	5	ACCGGGAATTCAAAAAGTTTT	TCCTAAGAGCAGCGTGTCTA	21	20	33.3	50.0	54.7	57.9	6.3	0.0	4.392932	114492	115350	626-949	1.000000	52.4
rps15-trnN(GUU)_p1	rps15-trnN(GUU)	1	CTGACAGCGTTTTCAAATCGA	AGCCCAGTTTATGAAGATTCT	21	21	42.9	38.1	57.5	54.6	4.8	0.0	5.933414	129396	129515	200-212	1.000000	48.9
rps15-trnN(GUU)_p2	rps15-trnN(GUU)	2	TCTGACAGCGTTTTCAAATCG	AGCCCAGTTTATGAAGATTCT	21	21	42.9	38.1	57.5	54.6	0.0	0.0	5.933414	129396	129515	201-213	1.000000	48.9
rps15-trnN(GUU)_p3	rps15-trnN(GUU)	3	CTGACAGCGTTTTCAAATCGA	AGCCCAGTTTATGAAGATTCTT	21	22	42.9	36.4	57.5	55.4	4.8	0.0	6.117379	129396	129515	200-212	1.000000	48.9
rps15-trnN(GUU)_p4	rps15-trnN(GUU)	4	TCTGACAGCGTTTTCAAATCG	AGCCCAGTTTATGAAGATTCTT	21	22	42.9	36.4	57.5	55.4	0.0	0.0	6.117379	129396	129515	201-213	1.000000	48.9
rps15-trnN(GUU)_p5	rps15-trnN(GUU)	5	TGACAGCGTTTTCAAATCGA	AGCCCAGTTTATGAAGATTCT	20	21	40.0	38.1	56.3	54.6	4.8	0.0	6.106227	129396	129515	199-211	1.000000	48.6