primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnKUUU-rps16_p1	trnKUUU-rps16	1	ACGCACTTAAAAGCCGAGTA	CTTGAAAAAGGCGCTCAACC	20	20	45.0	50.0	57.6	58.2	9.5	7.6	0.672931	4109	4866	892-963	1.000000	76.5
trnKUUU-rps16_p2	trnKUUU-rps16	2	AGTCGTGGTCTTACAAACCC	CTTGAAAAAGGCGCTCAACC	20	20	50.0	50.0	57.5	58.2	0.0	7.6	0.767555	4109	4866	966-1037	1.000000	75.6
trnKUUU-rps16_p3	trnKUUU-rps16	3	GTCGTGGTCTTACAAACCCT	CTTGAAAAAGGCGCTCAACC	20	20	50.0	50.0	57.5	58.2	0.0	7.6	0.767555	4109	4866	965-1036	1.000000	75.6
trnKUUU-rps16_p4	trnKUUU-rps16	4	AGACGCACTTAAAAGCCGAG	CTTGAAAAAGGCGCTCAACC	20	20	50.0	50.0	58.6	58.2	9.5	7.6	0.799581	4109	4866	894-965	1.000000	75.5
trnKUUU-rps16_p5	trnKUUU-rps16	5	ACGCACTTAAAAGCCGAGTA	AAGCGGGGGTTTTTACAGAA	20	20	45.0	45.0	57.6	57.6	9.5	0.0	0.812198	4109	4866	843-914	1.000000	75.5
trnLUAA-trnTUGU_p1	trnLUAA-trnTUGU	1	ACCAATTTCGCCATATCCCC	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.137364	16781	17892	1110-1162	1.000000	83.0
trnLUAA-trnTUGU_p2	trnLUAA-trnTUGU	2	TAGCGTCTACCAATTTCGCC	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.202173	16781	17892	1118-1170	1.000000	82.4
trnLUAA-trnTUGU_p3	trnLUAA-trnTUGU	3	ACCAATTTCGCCATATCCCC	GGAATCGAACCGATGACCAT	20	20	50.0	50.0	58.0	57.8	0.0	15.5	0.245117	16781	17892	1142-1194	1.000000	82.0
trnLUAA-trnTUGU_p4	trnLUAA-trnTUGU	4	TAGCGTCTACCAATTTCGCC	GGAATCGAACCGATGACCAT	20	20	50.0	50.0	58.1	57.8	0.0	15.5	0.309926	16781	17892	1150-1202	1.000000	81.5
trnLUAA-trnTUGU_p5	trnLUAA-trnTUGU	5	AGTCCGTAGCGTCTACCAAT	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.2	57.9	10.2	0.0	0.372548	16781	17892	1124-1176	1.000000	81.1
psbD-trnTGGU_p1	psbD-trnTGGU	1	GACCGGACCAACCTACAAAA	GTGGTAGAGTAACGCCATGG	20	20	50.0	55.0	57.7	58.1	9.5	7.6	0.323334	31912	33339	1558-1592	1.000000	79.9
psbD-trnTGGU_p2	psbD-trnTGGU	2	GACCGGACCAACCTACAAAA	TCAGTGGTAGAGTAACGCCA	20	20	50.0	50.0	57.7	58.1	9.5	0.0	0.345488	31912	33339	1561-1595	1.000000	79.7
psbD-trnTGGU_p3	psbD-trnTGGU	3	TAGACCGGACCAACCTACAA	GTGGTAGAGTAACGCCATGG	20	20	50.0	55.0	57.7	58.1	9.5	7.6	0.371563	31912	33339	1560-1594	1.000000	79.5
psbD-trnTGGU_p4	psbD-trnTGGU	4	TAGACCGGACCAACCTACAA	TCAGTGGTAGAGTAACGCCA	20	20	50.0	50.0	57.7	58.1	9.5	0.0	0.393718	31912	33339	1563-1597	1.000000	79.3
psbD-trnTGGU_p5	psbD-trnTGGU	5	GAGCAATAGACCGGACCAAC	GTGGTAGAGTAACGCCATGG	20	20	55.0	55.0	58.3	58.1	9.5	7.6	0.410916	31912	33339	1566-1600	1.000000	79.2
trnYGUA-trnDGUC_p1	trnYGUA-trnDGUC	1	TGGCAATATGTCTACGCTGG	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	57.8	58.5	0.0	7.1	0.716274	33859	34344	547-573	1.000000	76.2
trnYGUA-trnDGUC_p2	trnYGUA-trnDGUC	2	GGCAATATGTCTACGCTGGT	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	57.8	58.5	0.0	7.1	0.717825	33859	34344	546-572	1.000000	76.1
trnYGUA-trnDGUC_p3	trnYGUA-trnDGUC	3	GGGGACGGACTGTAAATTCG	CGGTGCTCTGACCAATTGAA	20	20	55.0	50.0	58.4	58.5	0.0	7.1	0.828828	33859	34344	568-594	1.000000	75.2
trnYGUA-trnDGUC_p4	trnYGUA-trnDGUC	4	GGGACGGACTGTAAATTCGT	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	57.6	58.5	0.0	7.1	0.852505	33859	34344	567-593	1.000000	75.0
trnYGUA-trnDGUC_p5	trnYGUA-trnDGUC	5	GATTCTTCCTGGGTCGATGC	CGGTGCTCTGACCAATTGAA	20	20	55.0	50.0	58.4	58.5	0.0	7.1	0.889785	33859	34344	601-627	1.000000	74.6
atpF_p1	atpF	1	TCAAAAAGAAATAGGAATAGCAA	TTCGTCGGCTCTAATGGTTG	23	20	26.1	50.0	52.3	58.0	0.0	0.0	8.663991	52721	54038	1488-1501	1.000000	40.9
atpF_p2	atpF	2	TTCAAAAAGAAATAGGAATAGCA	TTCGTCGGCTCTAATGGTTG	23	20	26.1	50.0	52.3	58.0	0.0	0.0	8.663991	52721	54038	1489-1502	1.000000	40.9
atpF_p3	atpF	3	TCAAAAAGAAATAGGAATAGCAA	GGCTCTAATGGTTGCCATGA	23	20	26.1	50.0	52.3	57.9	0.0	1.0	8.716545	52721	54038	1482-1495	1.000000	40.9
atpF_p4	atpF	4	TTCAAAAAGAAATAGGAATAGCA	GGCTCTAATGGTTGCCATGA	23	20	26.1	50.0	52.3	57.9	0.0	1.0	8.716545	52721	54038	1483-1496	1.000000	40.9
atpF_p5	atpF	5	AGAAAAAGTGTTTAAGTGATACA	TTCGTCGGCTCTAATGGTTG	23	20	26.1	50.0	52.3	58.0	0.0	0.0	8.743005	52721	54038	1460-1473	1.000000	40.9
psbI-psbK_p1	psbI-psbK	1	GGATTACGTCCTGGATCGTT	GTTTGGCAAGCTGCTGTAAG	20	20	50.0	50.0	57.1	57.9	22.6	1.4	0.940988	58162	58547	491-512	1.000000	76.9
psbI-psbK_p2	psbI-psbK	2	GGATTACGTCCTGGATCGTT	CCAAATTACCCGAGGCCTAC	20	20	50.0	55.0	57.1	58.0	22.6	4.4	0.921721	58162	58547	577-598	1.000000	76.8
psbI-psbK_p3	psbI-psbK	3	GGATTACGTCCTGGATCGTT	TTAGCCAAATTACCCGAGGC	20	20	50.0	50.0	57.1	58.0	22.6	0.0	0.931895	58162	58547	581-602	1.000000	76.7
psbI-psbK_p4	psbI-psbK	4	TCTTCACGTCCAGGATTACG	CCAAATTACCCGAGGCCTAC	20	20	50.0	55.0	57.1	58.0	0.0	4.4	0.970323	58162	58547	589-610	1.000000	76.4
psbI-psbK_p5	psbI-psbK	5	TCTTCACGTCCAGGATTACG	TTAGCCAAATTACCCGAGGC	20	20	50.0	50.0	57.1	58.0	0.0	0.0	0.980497	58162	58547	593-614	1.000000	76.3
trnQUUG-accD_p1	trnQUUG-accD	1	GAAATTGAAATGGGGCGTGG	CTTCCGCTTCCTCGTTAGAC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.098064	59153	61736	2323-2867	0.846154	85.1
trnQUUG-accD_p2	trnQUUG-accD	2	CCGTCGATATTTGCACTGGA	CTTCCGCTTCCTCGTTAGAC	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.154724	59153	61736	2390-2934	0.846154	84.6
trnQUUG-accD_p3	trnQUUG-accD	3	TCCGTCGATATTTGCACTGG	CTTCCGCTTCCTCGTTAGAC	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.154724	59153	61736	2391-2935	0.846154	84.6
trnQUUG-accD_p4	trnQUUG-accD	4	GAGGTTCGAATCCTTCCGTC	CTTCCGCTTCCTCGTTAGAC	20	20	55.0	55.0	58.1	58.1	16.9	0.0	0.165413	59153	61736	2265-2809	0.846154	84.5
trnQUUG-accD_p5	trnQUUG-accD	5	GAAATTGAAATGGGGCGTGG	TCTTCCGCTTCCTCGTTAGA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.178473	59153	61736	2324-2868	0.846154	84.4
accD-psaI_p1	accD-psaI	1	CCACGCAATCCTTTAAAGGG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.1	58.0	29.7	26.9	0.966463	63393	64009	740-770	1.000000	75.0
accD-psaI_p2	accD-psaI	2	CCACGCAATCCTTTAAAGGG	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	57.1	57.9	29.7	26.8	1.042997	63393	64009	746-776	1.000000	74.4
accD-psaI_p3	accD-psaI	3	CGATCAAATTGTACCACGCA	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	56.8	58.0	0.0	26.9	1.200011	63393	64009	753-783	1.000000	73.1
accD-psaI_p4	accD-psaI	4	CGATCAAATTGTACCACGCA	AAGAAGCCATTGCAATTGCC	20	20	45.0	45.0	56.8	57.9	0.0	26.8	1.276545	63393	64009	759-789	1.000000	72.5
accD-psaI_p5	accD-psaI	5	TCGATCAAATTGTACCACGCA	CCATTGCAATTGCCGGAAAT	21	20	42.9	45.0	58.3	58.0	0.0	26.9	1.337357	63393	64009	754-784	1.000000	72.0
ycf4-cemA_p1	ycf4-cemA	1	CCTTGACGCGTAGTGATGAT	CCGAGTGCGTAGTATTCCAC	20	20	50.0	55.0	57.8	58.2	14.0	0.0	0.373455	65078	65949	1043-1059	1.000000	79.0
ycf4-cemA_p2	ycf4-cemA	2	CCTTGACGCGTAGTGATGAT	CGAGTGCGTAGTATTCCACC	20	20	50.0	55.0	57.8	58.2	14.0	0.0	0.373455	65078	65949	1042-1058	1.000000	79.0
ycf4-cemA_p3	ycf4-cemA	3	CCTTGACGCGTAGTGATGAT	TCCGAGTGCGTAGTATTCCA	20	20	50.0	50.0	57.8	58.2	14.0	0.0	0.455908	65078	65949	1044-1060	1.000000	78.3
ycf4-cemA_p4	ycf4-cemA	4	TATTTCTTGCGCGTACCCAT	CCGAGTGCGTAGTATTCCAC	20	20	45.0	55.0	57.7	58.2	8.4	0.0	0.482753	65078	65949	978-994	1.000000	78.2
ycf4-cemA_p5	ycf4-cemA	5	TATTTCTTGCGCGTACCCAT	CGAGTGCGTAGTATTCCACC	20	20	45.0	55.0	57.7	58.2	8.4	0.0	0.482753	65078	65949	977-993	1.000000	78.2
petA-psbJ_p1	petA-psbJ	1	ATCCATTACGTGTCCAAGGC	AGCCGGTATTCTTGTGATCG	20	20	50.0	50.0	58.0	57.8	0.0	1.9	0.263455	67738	68770	1143-1272	1.000000	82.2
petA-psbJ_p2	petA-psbJ	2	ATCCATTACGTGTCCAAGGC	TACTCCGGGTTAGGCTCATC	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.337432	67738	68770	1094-1223	1.000000	81.6
petA-psbJ_p3	petA-psbJ	3	GGCCTTTTGTTCTTCTTGGC	AGCCGGTATTCTTGTGATCG	20	20	50.0	50.0	57.8	57.8	0.0	1.9	0.379922	67738	68770	1126-1255	1.000000	81.2
petA-psbJ_p4	petA-psbJ	4	CATTACGTGTCCAAGGCCTT	AGCCGGTATTCTTGTGATCG	20	20	50.0	50.0	58.2	57.8	13.8	1.9	0.403852	67738	68770	1140-1269	1.000000	81.0
petA-psbJ_p5	petA-psbJ	5	ATCCATTACGTGTCCAAGGC	ATACTCCGGGTTAGGCTCAT	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.438854	67738	68770	1095-1224	1.000000	80.8
trnICAU-ycf2_p1	trnICAU-ycf2	1	GTTGGGCGCTTTAACCATTC	AACGCAGGACACTCCAAATT	20	20	50.0	45.0	58.0	58.0	7.6	0.0	0.027944	90744	91327	678-826	1.000000	86.1
trnICAU-ycf2_p2	trnICAU-ycf2	2	GTTGGGCGCTTTAACCATTC	GCAGGAGCGAAACAATCAAC	20	20	50.0	50.0	58.0	58.0	7.6	0.0	0.040097	90744	91327	879-1027	1.000000	85.5
trnICAU-ycf2_p3	trnICAU-ycf2	3	GTTGGGCGCTTTAACCATTC	AGGAAGATCCAGAAAGGCCT	20	20	50.0	50.0	58.0	58.0	7.6	0.0	0.030840	90744	91327	1197-1345	1.000000	85.2
trnICAU-ycf2_p4	trnICAU-ycf2	4	CACTTGACTGTCTACGGCTC	AACGCAGGACACTCCAAATT	20	20	55.0	45.0	58.0	58.0	9.5	0.0	0.034771	90744	91327	1274-1422	1.000000	85.1
trnICAU-ycf2_p5	trnICAU-ycf2	5	CACTTGACTGTCTACGGCTC	AGGAAGATCCAGAAAGGCCT	20	20	55.0	50.0	58.0	58.0	9.5	0.0	0.037666	90744	91327	1793-1941	1.000000	84.7
rrn16-trnIGAU_p1	rrn16-trnIGAU	1	CAAGGTAGCCGTACTGGAAG	CTATCCATGTGGCTGAGAGC	20	20	55.0	55.0	57.7	57.8	7.8	0.0	0.462074	107472	108371	543-1045	1.000000	84.4
rrn16-trnIGAU_p2	rrn16-trnIGAU	2	CAAGGTAGCCGTACTGGAAG	CGCTGATGAGCACATTGAAC	20	20	55.0	50.0	57.7	57.8	7.8	4.7	0.464916	107472	108371	562-1064	1.000000	84.3
rrn16-trnIGAU_p3	rrn16-trnIGAU	3	CAAGGTAGCCGTACTGGAAG	GGCACAACGACGCAATTATC	20	20	55.0	50.0	57.7	58.2	7.8	0.0	0.485056	107472	108371	511-1013	1.000000	84.3
rrn16-trnIGAU_p4	rrn16-trnIGAU	4	AAGTCGTAACAAGGTAGCCG	CTATCCATGTGGCTGAGAGC	20	20	50.0	55.0	57.6	57.8	0.0	0.0	0.525760	107472	108371	552-1054	1.000000	83.8
rrn16-trnIGAU_p5	rrn16-trnIGAU	5	AAGTCGTAACAAGGTAGCCG	CGCTGATGAGCACATTGAAC	20	20	50.0	50.0	57.6	57.8	0.0	4.7	0.528602	107472	108371	571-1073	1.000000	83.7
ndhF-rpl32_p1	ndhF-rpl32	1	ACTGGTAGTGGAATGAAAGGT	TTTGATATAGAAGTGCGTTTTT	21	22	42.9	27.3	56.5	52.4	0.0	0.0	10.020343	117403	118282	937-1000	1.000000	45.3
ndhF-rpl32_p2	ndhF-rpl32	2	ACTGGTAGTGGAATGAAAGGT	TTTTGATATAGAAGTGCGTTTT	21	22	42.9	27.3	56.5	52.4	0.0	0.0	10.020343	117403	118282	938-1001	1.000000	45.3
ndhF-rpl32_p3	ndhF-rpl32	3	ACTGGTAGTGGAATGAAAGGT	TTTTTGATATAGAAGTGCGTTT	21	22	42.9	27.3	56.5	52.4	0.0	0.0	10.020343	117403	118282	939-1002	1.000000	45.3
ndhF-rpl32_p4	ndhF-rpl32	4	ACTGGTAGTGGAATGAAAGGT	TCCAAATAGTTTTACGAATACG	21	22	42.9	31.8	56.5	52.4	0.0	0.0	10.038344	117403	118282	963-1026	1.000000	45.3
ndhF-rpl32_p5	ndhF-rpl32	5	AGGAACTGGTAGTGGAATGA	TTTGATATAGAAGTGCGTTTTT	20	22	45.0	27.3	55.5	52.4	0.0	0.0	10.117756	117403	118282	941-1004	1.000000	45.3
ycf1-ndhF_p1	ycf1-ndhF	1	AGTGAGCAATGGGAAAGCAA	TACCATAGTAGCGGCATGGA	20	20	45.0	50.0	58.0	58.0	0.0	20.2	0.004289	136033	136289	1325-1524	1.000000	85.6
ycf1-ndhF_p2	ycf1-ndhF	2	TCCGCATTGACTTAGTGAGC	TACCATAGTAGCGGCATGGA	20	20	50.0	50.0	58.0	58.0	0.0	20.2	0.014082	136033	136289	1338-1537	1.000000	85.5
ycf1-ndhF_p3	ycf1-ndhF	3	AGTGAGCAATGGGAAAGCAA	GGCATGGATAAGAGCGGAAA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.033033	136033	136289	1313-1512	1.000000	85.4
ycf1-ndhF_p4	ycf1-ndhF	4	TCCAGCCCATTTCAGGACTA	TACCATAGTAGCGGCATGGA	20	20	50.0	50.0	58.0	58.0	0.0	20.2	0.038523	136033	136289	1529-1728	1.000000	85.2
ycf1-ndhF_p5	ycf1-ndhF	5	TGCCGATTCAACCTGTTTCA	TACCATAGTAGCGGCATGGA	20	20	45.0	50.0	58.0	58.0	0.0	20.2	0.029672	136033	136289	1694-1893	1.000000	84.9
trnIGAU-rrn16_p1	trnIGAU-rrn16	1	CTATCCATGTGGCTGAGAGC	CAAGGTAGCCGTACTGGAAG	20	20	55.0	55.0	57.8	57.7	0.0	7.8	0.462074	145204	146103	543-1045	1.000000	84.4
trnIGAU-rrn16_p2	trnIGAU-rrn16	2	GGCACAACGACGCAATTATC	CAAGGTAGCCGTACTGGAAG	20	20	50.0	55.0	58.2	57.7	0.0	7.8	0.485056	145204	146103	511-1013	1.000000	84.4
trnIGAU-rrn16_p3	trnIGAU-rrn16	3	CGCTGATGAGCACATTGAAC	CAAGGTAGCCGTACTGGAAG	20	20	50.0	55.0	57.8	57.7	4.7	7.8	0.464916	145204	146103	562-1064	1.000000	84.3
trnIGAU-rrn16_p4	trnIGAU-rrn16	4	CTATCCATGTGGCTGAGAGC	AAGTCGTAACAAGGTAGCCG	20	20	55.0	50.0	57.8	57.6	0.0	0.0	0.525760	145204	146103	552-1054	1.000000	83.9
trnIGAU-rrn16_p5	trnIGAU-rrn16	5	CGCTGATGAGCACATTGAAC	AAGTCGTAACAAGGTAGCCG	20	20	50.0	50.0	57.8	57.6	4.7	0.0	0.528602	145204	146103	571-1073	1.000000	83.8
ycf2_p1	ycf2	1	GCAGGAGCGAAACAATCAAC	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.0	58.0	0.0	7.6	0.040097	162040	162159	879-1027	1.000000	85.5
ycf2_p2	ycf2	2	CCCTCCTTTTTCCGATCCAG	GTTGGGCGCTTTAACCATTC	20	20	55.0	50.0	58.0	58.0	0.0	7.6	0.053324	162040	162159	987-1135	1.000000	85.3
ycf2_p3	ycf2	3	AGGAAGATCCAGAAAGGCCT	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.0	58.0	0.0	7.6	0.030840	162040	162159	1197-1345	1.000000	85.2
ycf2_p4	ycf2	4	GAATAGCCGGGACTTTGAGG	GTTGGGCGCTTTAACCATTC	20	20	55.0	50.0	58.0	58.0	2.2	7.6	0.054274	162040	162159	1214-1362	1.000000	85.0
ycf2_p5	ycf2	5	CCACAGTGGACCTCTCAGTA	GTTGGGCGCTTTAACCATTC	20	20	55.0	50.0	58.1	58.0	15.3	7.6	0.084470	162040	162159	1365-1513	1.000000	84.6
ycf2-trnICAU_p1	ycf2-trnICAU	1	AACGCAGGACACTCCAAATT	GTTGGGCGCTTTAACCATTC	20	20	45.0	50.0	58.0	58.0	0.0	7.6	0.027944	162248	162831	678-826	1.000000	86.1
ycf2-trnICAU_p2	ycf2-trnICAU	2	GCAGGAGCGAAACAATCAAC	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.0	58.0	0.0	7.6	0.040097	162248	162831	879-1027	1.000000	85.5
ycf2-trnICAU_p3	ycf2-trnICAU	3	AGGAAGATCCAGAAAGGCCT	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.0	58.0	0.0	7.6	0.030840	162248	162831	1197-1345	1.000000	85.2
ycf2-trnICAU_p4	ycf2-trnICAU	4	AACGCAGGACACTCCAAATT	CACTTGACTGTCTACGGCTC	20	20	45.0	55.0	58.0	58.0	0.0	9.5	0.034771	162248	162831	1274-1422	1.000000	85.1
ycf2-trnICAU_p5	ycf2-trnICAU	5	AGGAAGATCCAGAAAGGCCT	CACTTGACTGTCTACGGCTC	20	20	50.0	55.0	58.0	58.0	0.0	9.5	0.037666	162248	162831	1793-1941	1.000000	84.7