primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	ccsA	1	TGACGATCATAACAAAACGA	TTACATCGCCAACCGCTTTA	20	20	35.0	45.0	53.0	57.9	0.0	0.0	5.080174	69	188	628-628	0.250000	10.0
ccsA_p2	ccsA	2	TGACGATCATAACAAAACGA	GGCAAAATTCCGGGCATTTT	20	20	35.0	45.0	53.0	58.2	0.0	11.0	5.164026	69	188	793-793	0.250000	10.0
ccsA_p3	ccsA	3	TGACGATCATAACAAAACGA	TACATCGCCAACCGCTTTAT	20	20	35.0	45.0	53.0	57.7	0.0	0.0	5.303091	69	188	627-627	0.250000	10.0
ccsA_p4	ccsA	4	ATGACGATCATAACAAAACGA	TTACATCGCCAACCGCTTTA	21	20	33.3	45.0	53.7	57.9	0.0	0.0	5.410012	69	188	629-629	0.250000	10.0
ccsA_p5	ccsA	5	TGACGATCATAACAAAACGAT	TTACATCGCCAACCGCTTTA	21	20	33.3	45.0	53.7	57.9	0.0	0.0	5.410012	69	188	628-628	0.250000	10.0
orf274-atpI_p1	orf274-atpI	1	CAGAGTGCTTATCTGGCTGG	TCATTAGTAGGGGCCGCTAA	20	20	55.0	50.0	58.0	57.9	5.7	29.4	0.140270	30422	31806	2272-2431	0.500000	59.9
orf274-atpI_p2	orf274-atpI	2	CACGTTAGGTGGAGGTTCAG	TCATTAGTAGGGGCCGCTAA	20	20	55.0	50.0	57.9	57.9	0.0	29.4	0.171384	30422	31806	2471-2630	0.500000	59.7
orf274-atpI_p3	orf274-atpI	3	CGTTAGGTGGAGGTTCAGTG	TCATTAGTAGGGGCCGCTAA	20	20	55.0	50.0	57.9	57.9	0.0	29.4	0.171384	30422	31806	2469-2628	0.500000	59.7
orf274-atpI_p4	orf274-atpI	4	CAGAGTGCTTATCTGGCTGG	CCCCCAAAACGATCCATGAA	20	20	55.0	50.0	58.0	58.2	5.7	0.0	0.203165	30422	31806	2016-2175	0.500000	59.5
orf274-atpI_p5	orf274-atpI	5	CAGAGTGCTTATCTGGCTGG	AATCGCCACGGTAATAGAGC	20	20	55.0	50.0	58.0	57.8	5.7	0.0	0.199569	30422	31806	2230-2389	0.500000	59.4
trnG(ucc)-trnMe(cau)_p1	trnG(ucc)-trnMe(cau)	1	CGACATTGGACTTGGAAGGA	GGATGCTCTGACCAACTGAG	20	20	50.0	55.0	57.5	58.0	0.0	0.0	0.494051	46245	46390	225-252	1.000000	88.0
trnG(ucc)-trnMe(cau)_p2	trnG(ucc)-trnMe(cau)	2	CGACATTGGACTTGGAAGGA	ATGAAACGGATGCTCTGACC	20	20	50.0	50.0	57.5	58.0	0.0	0.0	0.502534	46245	46390	232-259	1.000000	88.0
trnG(ucc)-trnMe(cau)_p3	trnG(ucc)-trnMe(cau)	3	CGACATTGGACTTGGAAGGA	CTAGTGACATCCCGCGTATG	20	20	50.0	55.0	57.5	57.9	0.0	15.0	0.524574	46245	46390	249-276	1.000000	87.8
trnG(ucc)-trnMe(cau)_p4	trnG(ucc)-trnMe(cau)	4	CGACATTGGACTTGGAAGGA	ACCAACTGAGCTAAACAGGC	20	20	50.0	50.0	57.5	58.1	0.0	5.9	0.575136	46245	46390	215-242	1.000000	87.4
trnG(ucc)-trnMe(cau)_p5	trnG(ucc)-trnMe(cau)	5	ACATTGGACTTGGAAGGACC	GGATGCTCTGACCAACTGAG	20	20	50.0	55.0	57.4	58.0	0.0	0.0	0.608004	46245	46390	223-250	1.000000	87.1
ycf1-ycf4_p1	ycf1-ycf4	1	GTCATTGTTCCAATCGCAGC	GGAATGTTGGAGGTGGGTTT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.109444	56941	57463	1310-1316	0.500000	60.7
ycf1-ycf4_p2	ycf1-ycf4	2	GTCATTGTTCCAATCGCAGC	TGTTGGAGGTGGGTTTAACG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.129671	56941	57463	1306-1312	0.500000	60.6
ycf1-ycf4_p3	ycf1-ycf4	3	GCAGTCATTGTTCCAATCGC	GGAATGTTGGAGGTGGGTTT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.109444	56941	57463	1313-1313	0.250000	49.1
ycf1-ycf4_p4	ycf1-ycf4	4	GCAGTCATTGTTCCAATCGC	TGTTGGAGGTGGGTTTAACG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.129671	56941	57463	1309-1309	0.250000	49.0
ycf1-ycf4_p5	ycf1-ycf4	5	CGCAGTCATTGTTCCAATCG	GGAATGTTGGAGGTGGGTTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.173507	56941	57463	1314-1314	0.250000	48.6
psbI_p1	psbI	1	CGATATATCGGACCCAATGGG	ACCAAGCAAAGCTTCTACGA	21	20	52.4	45.0	57.8	57.5	24.4	0.0	1.723220	60094	62608	2988-2988	0.250000	36.2
psbI_p2	psbI	2	CGATATATCGGACCCAATGGG	AACCAAGCAAAGCTTCTACGA	21	21	52.4	42.9	57.8	58.2	24.4	0.0	2.333250	60094	62608	2989-2989	0.250000	31.3
psbI_p3	psbI	3	CGATATATCGGACCCAATGGG	ACCAAGCAAAGCTTCTACGAA	21	21	52.4	42.9	57.8	58.2	24.4	0.0	2.333250	60094	62608	2988-2988	0.250000	31.3
psbI_p4	psbI	4	CGATATATCGGACCCAATGGG	AACCAAGCAAAGCTTCTACG	21	20	52.4	45.0	57.8	56.6	24.4	0.0	2.530630	60094	62608	2989-2989	0.250000	29.8
psbI_p5	psbI	5	CGATATATCGGACCCAATGGG	AAACCAAGCAAAGCTTCTACG	21	21	52.4	42.9	57.8	57.4	24.4	0.0	2.805471	60094	62608	2990-2990	0.250000	27.6
orf458_p1	orf458	1	GCAGATTTTCGCAAGGCATT	AAAGCCAATGGTACCTAGCG	20	20	45.0	50.0	58.0	58.0	0.0	14.4	0.038816	60573	61949	1782-1962	0.750000	81.7
orf458_p2	orf458	2	GCAGATTTTCGCAAGGCATT	GTACCCAATTTCAGGTGGCA	20	20	45.0	50.0	58.0	58.1	0.0	2.5	0.082557	60573	61949	2148-2325	0.750000	81.3
orf458_p3	orf458	3	GCAGATTTTCGCAAGGCATT	GAGCTGGTGGATTTTCCGAA	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.179213	60573	61949	1647-1828	0.750000	80.6
orf458_p4	orf458	4	CCCCTAAGAACCGTACATGC	AAAGCCAATGGTACCTAGCG	20	20	55.0	50.0	58.1	58.0	0.0	14.4	0.088922	60573	61949	1929-2005	0.500000	61.1
orf458_p5	orf458	5	CCCCTAAGAACCGTACATGC	GTACCCAATTTCAGGTGGCA	20	20	55.0	50.0	58.1	58.1	0.0	2.5	0.132663	60573	61949	2292-2368	0.500000	60.7
orf458-rps12_p1	orf458-rps12	1	TCGATTTGAGTGCGCTGTTA	TTTAGTTCGTGGTGGTCGTG	20	20	45.0	50.0	57.9	58.1	3.4	0.0	0.217603	61950	62873	1261-1268	0.500000	59.8
orf458-rps12_p2	orf458-rps12	2	TCGATTTGAGTGCGCTGTTA	CGTGGTGGTCGTGTAAAAGA	20	20	45.0	50.0	57.9	58.1	3.4	0.0	0.217603	61950	62873	1254-1261	0.500000	59.7
orf458-rps12_p3	orf458-rps12	3	TCGATTTGAGTGCGCTGTTA	TCGTGGTGGTCGTGTAAAAG	20	20	45.0	50.0	57.9	58.1	3.4	0.0	0.217603	61950	62873	1255-1262	0.500000	59.7
orf458-rps12_p4	orf458-rps12	4	GGGTTCGAGGGCTGTTTTTA	CGTGGTGGTCGTGTAAAAGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.244241	61950	62873	1524-1531	0.500000	59.7
orf458-rps12_p5	orf458-rps12	5	GGGTTCGAGGGCTGTTTTTA	TCGTGGTGGTCGTGTAAAAG	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.244241	61950	62873	1525-1532	0.500000	59.7
orf212_p1	orf212	1	TCCAATGGTCTTGGGGATCT	AAGTCGGAGTTCCTGAGACA	20	20	50.0	50.0	58.0	58.0	0.0	7.2	0.017580	87459	88100	1227-1227	0.250000	49.9
orf212_p2	orf212	2	TCCAATGGTCTTGGGGATCT	ACAAGTCGGAGTTCCTGAGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.017580	87459	88100	1229-1229	0.250000	49.9
orf212_p3	orf212	3	TCCAATGGTCTTGGGGATCT	AGTCGGAGTTCCTGAGACAA	20	20	50.0	50.0	58.0	58.0	0.0	7.2	0.017580	87459	88100	1226-1226	0.250000	49.9
orf212_p4	orf212	4	TCCAATGGTCTTGGGGATCT	ACTACGTTGAATCGCTGGAC	20	20	50.0	50.0	58.0	58.0	0.0	3.4	0.022209	87459	88100	921-921	0.250000	49.8
orf212_p5	orf212	5	TCCAATGGTCTTGGGGATCT	AAGACCCTTTTGGACTGCAG	20	20	50.0	50.0	58.0	58.0	0.0	13.5	0.027496	87459	88100	1317-1317	0.250000	49.8
orf258-orf532_p1	orf258-orf532	1	ACACACAAAGGTCTTTCGCT	TCAAAATGGGGTGTCTAAGCA	20	21	45.0	42.9	58.0	57.5	0.0	0.0	1.555683	97798	97917	709-709	0.250000	37.6
orf258-orf532_p2	orf258-orf532	2	CGGGTAATACTCAGCCAACC	TCAAAATGGGGTGTCTAAGCA	20	21	55.0	42.9	58.1	57.5	0.0	0.0	1.567831	97798	97917	919-919	0.250000	37.5
orf258-orf532_p3	orf258-orf532	3	CACACACAAAGGTCTTTCGC	TCAAAATGGGGTGTCTAAGCA	20	21	50.0	42.9	57.9	57.5	0.0	0.0	1.622619	97798	97917	710-710	0.250000	37.0
orf258-orf532_p4	orf258-orf532	4	ACACACAAAGGTCTTTCGCT	TGGGGTGTCTAAGCAAAAAGA	20	21	45.0	42.9	58.0	57.4	0.0	0.0	1.624857	97798	97917	703-703	0.250000	37.0
orf258-orf532_p5	orf258-orf532	5	CGGGTAATACTCAGCCAACC	TGGGGTGTCTAAGCAAAAAGA	20	21	55.0	42.9	58.1	57.4	0.0	0.0	1.637005	97798	97917	913-913	0.250000	36.9
orf532_p1	orf532	1	GAATTCTACCACACACGGGG	ACAAGTTTTGCAACGTAGCG	20	20	55.0	45.0	58.3	57.9	0.0	0.0	0.365470	98151	99752	2594-2594	0.250000	47.1
orf532_p2	orf532	2	CGGTTGAATGCGGCATTAAA	ACAAGTTTTGCAACGTAGCG	20	20	45.0	45.0	57.7	57.9	26.7	0.0	0.369908	98151	99752	2615-2615	0.250000	47.0
orf532_p3	orf532	3	GCCGTAAATCCTGAACCGAA	ACAAGTTTTGCAACGTAGCG	20	20	50.0	45.0	58.3	57.9	0.0	0.0	0.370350	98151	99752	2782-2782	0.250000	47.0
orf532_p4	orf532	4	ACACGGGGAATTAAAAGGCA	ACAAGTTTTGCAACGTAGCG	20	20	45.0	45.0	57.7	57.9	0.0	0.0	0.395706	98151	99752	2582-2582	0.250000	46.8
orf532_p5	orf532	5	GAATTCTACCACACACGGGG	CAAGTTTTGCAACGTAGCGA	20	20	55.0	45.0	58.3	57.6	0.0	0.0	0.642752	98151	99752	2593-2593	0.250000	44.9
trnL(caa)-psaA_p1	trnL(caa)-psaA	1	TACCGTTCCATCATAAGGGC	ACTCTTTTTGCTTCACTTTCTGG	20	23	50.0	39.1	57.1	57.7	0.0	0.0	4.177078	114909	115496	646-704	1.000000	58.6
trnL(caa)-psaA_p2	trnL(caa)-psaA	2	TACCGTTCCATCATAAGGGC	CTCTTTTTGCTTCACTTTCTGGA	20	23	50.0	39.1	57.1	57.5	0.0	0.0	4.427913	114909	115496	645-703	1.000000	56.6
trnL(caa)-psaA_p3	trnL(caa)-psaA	3	TACCGTTCCATCATAAGGGC	TCTTTTTGCTTCACTTTCTGGAG	20	23	50.0	39.1	57.1	57.5	0.0	0.0	4.427913	114909	115496	644-702	1.000000	56.6
trnL(caa)-psaA_p4	trnL(caa)-psaA	4	CTACCGTTCCATCATAAGGGC	ACTCTTTTTGCTTCACTTTCTGG	21	23	52.4	39.1	58.2	57.7	0.0	0.0	4.486834	114909	115496	647-705	1.000000	56.1
trnL(caa)-psaA_p5	trnL(caa)-psaA	5	TACCGTTCCATCATAAGGGC	TCTTTTTGCTTCACTTTCTGGA	20	22	50.0	36.4	57.1	56.4	0.0	0.0	4.526887	114909	115496	644-702	1.000000	55.8
psaC_p1	psaC	1	TGGTTCGAAGCCAGAAACTC	GGGACTGATTTACCACCAGG	20	20	50.0	55.0	58.1	57.6	23.5	0.0	0.541105	125749	127621	2788-2825	0.500000	58.3
psaC_p2	psaC	2	ATGGGTGCGACTCGTTTAAA	GGGACTGATTTACCACCAGG	20	20	45.0	55.0	57.8	57.6	0.0	0.0	0.592910	125749	127621	2942-2979	0.500000	58.2
psaC_p3	psaC	3	TGGGTGCGACTCGTTTAAAT	GGGACTGATTTACCACCAGG	20	20	45.0	55.0	57.8	57.6	0.0	0.0	0.592910	125749	127621	2941-2978	0.500000	58.2
psaC_p4	psaC	4	GGTTCGAAGCCAGAAACTCT	GGGACTGATTTACCACCAGG	20	20	50.0	55.0	57.8	57.6	16.4	0.0	0.591399	125749	127621	2787-2824	0.500000	57.9
psaC_p5	psaC	5	AGTATATGGGTGCGACTCGT	GGGACTGATTTACCACCAGG	20	20	50.0	55.0	58.3	57.6	0.0	0.0	0.731286	125749	127621	2947-2984	0.500000	57.1
rpoA_p1	rpoA	1	CTTTCCTTTTGTCGTGCTGC	CCAGGGGTCATCAAATCGAG	20	20	50.0	55.0	58.2	58.0	0.0	0.0	0.282175	159073	160320	2266-2276	0.500000	60.6
rpoA_p2	rpoA	2	CTTTCCTTTTGTCGTGCTGC	GAATGCCCTCAGTCGGATTT	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.285340	159073	160320	2123-2132	0.500000	60.6
rpoA_p3	rpoA	3	CTTTCCTTTTGTCGTGCTGC	TCCGCTGAGCTCCATTAAAC	20	20	50.0	50.0	58.2	58.0	0.0	12.1	0.265392	159073	160320	2631-2641	0.500000	60.3
rpoA_p4	rpoA	4	CTTTCCTTTTGTCGTGCTGC	GTATGAATGCCCTCAGTCGG	20	20	50.0	55.0	58.2	58.1	0.0	0.0	0.370137	159073	160320	2127-2136	0.500000	59.9
rpoA_p5	rpoA	5	CTTTCCTTTTGTCGTGCTGC	TTGAACGTGAAGTGCGTACA	20	20	50.0	45.0	58.2	58.1	0.0	6.4	0.317318	159073	160320	2182-2182	0.250000	47.5
orf532-orf258_p1	orf532-orf258	1	AGCGAAAGACCTTTGTGTGT	ACGACAAACTGCGCTCTATT	20	20	45.0	45.0	58.0	57.9	0.0	9.7	0.118673	178653	178772	1334-1334	0.250000	49.1
orf532-orf258_p2	orf532-orf258	2	AGCGAAAGACCTTTGTGTGT	ATTACGACAAACTGCGCTCT	20	20	45.0	45.0	58.0	57.9	0.0	9.7	0.118673	178653	178772	1337-1337	0.250000	49.1
orf532-orf258_p3	orf532-orf258	3	GGTTGGCTGAGTATTACCCG	ACGACAAACTGCGCTCTATT	20	20	55.0	45.0	58.1	57.9	0.0	9.7	0.130821	178653	178772	1124-1124	0.250000	49.0
orf532-orf258_p4	orf532-orf258	4	GGTTGGCTGAGTATTACCCG	ATTACGACAAACTGCGCTCT	20	20	55.0	45.0	58.1	57.9	0.0	9.7	0.130821	178653	178772	1127-1127	0.250000	49.0
orf532-orf258_p5	orf532-orf258	5	AGCGAAAGACCTTTGTGTGT	TACTTGGCGCAACATTTCCT	20	20	45.0	45.0	58.0	58.1	0.0	10.8	0.134100	178653	178772	1525-1525	0.250000	48.9
orf258_p1	orf258	1	AACGCAATTGAAAACCCGAC	CAATCCTTGGAGATGCCGAA	20	20	45.0	50.0	57.9	58.0	10.3	0.0	0.171053	182509	183288	1462-1462	0.250000	48.6
orf258_p2	orf258	2	AACGCAATTGAAAACCCGAC	CAACAATTTCCGACTGCGAC	20	20	45.0	50.0	57.9	58.0	10.3	0.0	0.177896	182509	183288	1595-1595	0.250000	48.6
orf258_p3	orf258	3	AACGCAATTGAAAACCCGAC	TGCCGAAGTTCTAGCTGTTC	20	20	45.0	50.0	57.9	57.9	10.3	13.8	0.208653	182509	183288	1449-1449	0.250000	48.3
orf258_p4	orf258	4	AACGCAATTGAAAACCCGAC	TCCGACTGCGACAATTTGAT	20	20	45.0	45.0	57.9	57.9	10.3	2.8	0.221674	182509	183288	1587-1587	0.250000	48.2
orf258_p5	orf258	5	AACGCAATTGAAAACCCGAC	GTGAGCACCAAATTCATCGC	20	20	45.0	50.0	57.9	58.1	10.3	0.0	0.228130	182509	183288	1492-1492	0.250000	48.2
orf258-orf212_p1	orf258-orf212	1	TAAAACGGCCAATTGTCCCA	CCGTTAAATCCAGCAGTCGA	20	20	45.0	50.0	58.0	58.0	2.2	0.0	0.007784	185375	185524	925-925	0.250000	49.9
orf258-orf212_p2	orf258-orf212	2	TAAAACGGCCAATTGTCCCA	TCCGTTAAATCCAGCAGTCG	20	20	45.0	50.0	58.0	58.0	2.2	0.0	0.007784	185375	185524	926-926	0.250000	49.9
orf258-orf212_p3	orf258-orf212	3	TAAAACGGCCAATTGTCCCA	GATGCAAAACTGATTGGGGC	20	20	45.0	50.0	58.0	58.0	2.2	0.0	0.017537	185375	185524	490-490	0.250000	49.9
orf258-orf212_p4	orf258-orf212	4	TAAAACGGCCAATTGTCCCA	AGAAGGTCCGGGTGACTTTA	20	20	45.0	50.0	58.0	58.0	2.2	0.0	0.023009	185375	185524	899-899	0.250000	49.8
orf258-orf212_p5	orf258-orf212	5	TAAAACGGCCAATTGTCCCA	AAAAAGAAGGTCCGGGTGAC	20	20	45.0	50.0	58.0	58.0	2.2	0.0	0.026145	185375	185524	903-903	0.250000	49.8
orf212-psbA_p1	orf212-psbA	1	TGATTTGTACACGCCGGTAG	ACAACGTCAGTGTTTCTCCC	20	20	50.0	50.0	58.0	58.1	1.9	0.0	0.051486	187895	189333	2387-2387	0.250000	49.6
orf212-psbA_p2	orf212-psbA	2	CATCGACCGTGTCACATTCT	ACAACGTCAGTGTTTCTCCC	20	20	50.0	50.0	58.0	58.1	3.4	0.0	0.054243	187895	189333	2236-2236	0.250000	49.6
orf212-psbA_p3	orf212-psbA	3	TTCAACGCCAGCTATCTGAC	ACAACGTCAGTGTTTCTCCC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.060680	187895	189333	2605-2605	0.250000	49.5
orf212-psbA_p4	orf212-psbA	4	CCGGTAGATTCCTACGAGGT	ACAACGTCAGTGTTTCTCCC	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.072080	187895	189333	2374-2374	0.250000	49.4
orf212-psbA_p5	orf212-psbA	5	ACCACAGTGGGGTAATAGCT	ACAACGTCAGTGTTTCTCCC	20	20	50.0	50.0	58.0	58.1	17.8	0.0	0.083690	187895	189333	2328-2328	0.250000	49.3