primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	TTCCCGTTTCAGGCACTAAG	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	57.8	58.0	0.0	7.6	0.203252	4071	4725	967-1158	1.000000	88.8
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	TTCCCGTTTCAGGCACTAAG	CGGTCACGAAGAGAAGGAAG	20	20	50.0	55.0	57.8	58.0	0.0	0.0	0.194940	4071	4725	1067-1258	1.000000	88.6
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TTCCCGTTTCAGGCACTAAG	CGAGCCGTACGAAGAGAAAA	20	20	50.0	50.0	57.8	58.0	0.0	18.8	0.205774	4071	4725	1159-1350	1.000000	88.3
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	TTCCCGTTTCAGGCACTAAG	TTTTCTCGAGCCGTACGAAG	20	20	50.0	50.0	57.8	58.0	0.0	18.8	0.205774	4071	4725	1165-1356	1.000000	88.3
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	ACTCGTGGCCATCTCCATAT	CGGTCACGAAGAGAAGGAAG	20	20	50.0	55.0	58.3	58.0	9.7	0.0	0.293994	4071	4725	1159-1350	1.000000	87.6
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	ATAAAACGGATTCGCGTGGA	CGAAAACTGACAGCAGCTTG	20	20	45.0	50.0	58.0	58.0	5.8	1.2	0.041224	5799	6404	1046-1362	1.000000	91.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	ATAAAACGGATTCGCGTGGA	TCGAAAACTGACAGCAGCTT	20	20	45.0	45.0	58.0	58.0	5.8	1.2	0.059378	5799	6404	1047-1363	1.000000	91.3
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CCCAATAAAACGGATTCGCG	CGAAAACTGACAGCAGCTTG	20	20	50.0	50.0	57.9	58.0	0.0	1.2	0.127920	5799	6404	1050-1366	1.000000	90.7
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CCACAACGGATCATGTCCTT	CGAAAACTGACAGCAGCTTG	20	20	50.0	50.0	57.9	58.0	11.1	1.2	0.142147	5799	6404	967-1283	1.000000	90.4
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	ATAAAACGGATTCGCGTGGA	GCTATTCGGAGGTTCGAGTC	20	20	45.0	55.0	58.0	57.9	5.8	0.0	0.135500	5799	6404	788-788	0.333333	53.2
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.2	55.5	0.0	0.0	3.687191	7703	8197	618-882	1.000000	58.5
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	ACGGAAAGAGAGGGATTCGA	AACGAATCACACTTTTACCACT	20	22	50.0	36.4	58.2	56.2	0.0	0.0	3.927661	7703	8197	619-883	1.000000	56.5
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CTTTCGTCCACTCAGCCATC	ACGAATCACACTTTTACCACT	20	21	55.0	38.1	58.6	55.5	0.0	0.0	4.172099	7703	8197	554-818	1.000000	55.3
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	ACGGAAAGAGAGGGATTCGA	TACCACTAAACTATACCCGC	20	20	50.0	45.0	58.2	54.0	0.0	0.0	4.162424	7703	8197	604-868	1.000000	54.8
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCAC	20	20	50.0	40.0	58.2	53.9	0.0	0.0	4.276197	7703	8197	618-882	1.000000	53.8
atpF-atpH_p1	atpF-atpH	1	CCAGTGACCCAAGAAAACGA	CCGAGGGAAAAATACGAGGT	20	20	50.0	50.0	58.0	57.3	0.0	0.0	0.724056	12112	12584	522-633	1.000000	84.4
atpF-atpH_p2	atpF-atpH	2	CCAGTGACCCAAGAAAACGA	GCCGAGGGAAAAATACGAGG	20	20	50.0	55.0	58.0	58.7	0.0	0.0	0.741479	12112	12584	523-634	1.000000	84.3
atpF-atpH_p3	atpF-atpH	3	CCAGTGACCCAAGAAAACGA	CGAGGCCGAGGGAAAAATAC	20	20	50.0	55.0	58.0	58.7	0.0	0.0	0.741479	12112	12584	527-638	1.000000	84.2
atpF-atpH_p4	atpF-atpH	4	CCAGTGACCCAAGAAAACGA	GAGGCCGAGGGAAAAATACG	20	20	50.0	55.0	58.0	58.7	0.0	0.0	0.741479	12112	12584	526-637	1.000000	84.2
atpF-atpH_p5	atpF-atpH	5	CCAGTGACCCAAGAAAACGA	GGCCGAGGGAAAAATACGAG	20	20	50.0	55.0	58.0	58.7	0.0	0.0	0.741479	12112	12584	524-635	1.000000	84.2
trnG-GCC-trnfM-CAU_p1	trnG-GCC-trnfM-CAU	1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	57.8	58.0	0.0	4.2	0.208039	33902	34093	243-277	1.000000	88.1
trnG-GCC-trnfM-CAU_p2	trnG-GCC-trnfM-CAU	2	TCTTTGCCAAGGAGAAGACG	GTCACGGGTTCAAATCCTGT	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.277623	33902	34093	237-271	1.000000	87.6
trnG-GCC-trnfM-CAU_p3	trnG-GCC-trnfM-CAU	3	TCTTTGCCAAGGAGAAGACG	TCACGGGTTCAAATCCTGTC	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.348744	33902	34093	236-270	1.000000	87.1
trnG-GCC-trnfM-CAU_p4	trnG-GCC-trnfM-CAU	4	TCTTTGCCAAGGAGAAGACG	CACGGGTTCAAATCCTGTCT	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.354836	33902	34093	235-269	1.000000	87.1
trnG-GCC-trnfM-CAU_p5	trnG-GCC-trnfM-CAU	5	TCTTTGCCAAGGAGAAGACG	TGAGGTCACGGGTTCAAATC	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.348744	33902	34093	241-275	1.000000	87.0
psaA-ycf3_p1	psaA-ycf3	1	GCATCAGCATGTAGGTTCCA	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.083644	39224	39946	973-1234	1.000000	88.7
psaA-ycf3_p2	psaA-ycf3	2	GGCACTAGGTCCAATGTGAG	ATCAAGCCGCTGAGTATTGG	20	20	55.0	50.0	58.0	58.0	11.5	0.0	0.070797	39224	39946	1151-1412	1.000000	87.9
psaA-ycf3_p3	psaA-ycf3	3	ATCGCTTAGCCATGCTTCAA	ATCAAGCCGCTGAGTATTGG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.083644	39224	39946	1127-1388	1.000000	87.9
psaA-ycf3_p4	psaA-ycf3	4	GCCTACGATTGGCCAAACTA	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	58.0	58.0	24.7	0.0	0.073639	39224	39946	1175-1436	1.000000	87.7
psaA-ycf3_p5	psaA-ycf3	5	TGAGTAGGATCGCTTAGCCA	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.108324	39224	39946	1135-1396	1.000000	87.7
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	AGCCAATTAAGTCCGTAGCG	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.196808	43389	44028	484-720	1.000000	89.1
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	AGCCAATTAAGTCCGTAGCG	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.304561	43389	44028	516-752	1.000000	87.9
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	43389	44028	472-708	1.000000	87.7
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	GCCAATTAAGTCCGTAGCGT	20	20	55.0	50.0	57.9	58.4	0.0	0.0	0.484236	43389	44028	483-719	1.000000	86.8
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	43389	44028	504-740	1.000000	86.6
psaJ-rpl33_p1	psaJ-rpl33	1	TTTTCCCAGATGCGTTGACA	CGGACATCTTTCCCCTTAGC	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.070808	61189	61583	434-465	1.000000	91.0
psaJ-rpl33_p2	psaJ-rpl33	2	TTGGGTCTTTAGCCGGTCTA	CGGACATCTTTCCCCTTAGC	20	20	50.0	55.0	58.1	58.0	1.9	0.0	0.103696	61189	61583	473-504	1.000000	90.3
psaJ-rpl33_p3	psaJ-rpl33	3	CGAACTGAACTAAGAGCGCT	CGGACATCTTTCCCCTTAGC	20	20	50.0	55.0	58.0	58.0	11.3	0.0	0.061101	61189	61583	1064-1105	1.000000	89.1
psaJ-rpl33_p4	psaJ-rpl33	4	GCGTTCTACCGAACTGAACT	CGGACATCTTTCCCCTTAGC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.106813	61189	61583	1073-1114	1.000000	88.7
psaJ-rpl33_p5	psaJ-rpl33	5	CTGCGCTACATCCCTTTCAT	CGGACATCTTTCCCCTTAGC	20	20	50.0	55.0	58.0	58.0	9.7	0.0	0.086031	61189	61583	799-844	0.833333	81.9
ndhB_p1	ndhB	1	GTCTCGCGTGTCTACCATTT	ATGGCAGAGGCAAATAGAGC	20	20	50.0	50.0	58.0	58.0	5.8	0.0	0.029376	87171	88094	1684-2344	1.000000	91.8
ndhB_p2	ndhB	2	CTCGCGTGTCTACCATTTCA	ATGGCAGAGGCAAATAGAGC	20	20	50.0	50.0	58.0	58.0	5.8	0.0	0.030746	87171	88094	1682-2342	1.000000	91.8
ndhB_p3	ndhB	3	AGATGGAATGGAACGACGTG	ATGGCAGAGGCAAATAGAGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.031691	87171	88094	1346-2120	1.000000	91.7
ndhB_p4	ndhB	4	GTCTCGCGTGTCTACCATTT	TCCGAATTAGTGGATGCTGC	20	20	50.0	50.0	58.0	58.0	5.8	0.0	0.054271	87171	88094	1750-2410	1.000000	91.6
ndhB_p5	ndhB	5	GAGTCGGACATCCAATTGCT	ATGGCAGAGGCAAATAGAGC	20	20	50.0	50.0	58.0	58.0	11.3	0.0	0.051384	87171	88094	1540-2163	0.833333	84.9
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	ACCAATGGGATTGAAGAGCC	CTGCATAACCCAGGACCATC	20	20	50.0	55.0	57.9	58.0	3.6	0.0	0.177101	111739	112250	1930-1940	0.333333	53.4
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	ACCAATGGGATTGAAGAGCC	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	57.9	57.9	3.6	0.0	0.274250	111739	112250	1283-1293	0.333333	53.4
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	ACCAATGGGATTGAAGAGCC	ATCCGCATAAAAGAGCTGCA	20	20	50.0	45.0	57.9	58.0	3.6	1.0	0.192659	111739	112250	1945-1955	0.333333	53.3
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	ACCAATGGGATTGAAGAGCC	TCCGCATAAAAGAGCTGCAT	20	20	50.0	45.0	57.9	58.0	3.6	1.0	0.192659	111739	112250	1944-1954	0.333333	53.3
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	ACCAATGGGATTGAAGAGCC	AAAGAGCTGCATAACCCAGG	20	20	50.0	50.0	57.9	57.9	3.6	1.0	0.284762	111739	112250	1936-1946	0.333333	52.5
ccsA-psaC_p1	ccsA-psaC	1	GATGGTCCTGGGTTATGCAG	ATACCTTGGGACGGGTGTAA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.071677	113444	114489	1284-2425	1.000000	91.4
ccsA-psaC_p2	ccsA-psaC	2	TGCAGCTCTTTTATGCGGAT	ATACCTTGGGACGGGTGTAA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.087235	113444	114489	1269-2410	1.000000	91.3
ccsA-psaC_p3	ccsA-psaC	3	GAGCAGTATGGGCCAATGAA	ATACCTTGGGACGGGTGTAA	20	20	50.0	50.0	57.9	58.0	11.9	0.0	0.101686	113444	114489	988-2129	1.000000	91.2
ccsA-psaC_p4	ccsA-psaC	4	ATGCAGCTCTTTTATGCGGA	ATACCTTGGGACGGGTGTAA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.087235	113444	114489	1270-2411	0.833333	83.9
ccsA-psaC_p5	ccsA-psaC	5	GATGGTCCTGGGTTATGCAG	TCGGAATCTATGGACCGGAA	20	20	55.0	50.0	58.0	57.9	0.0	9.5	0.103335	113444	114489	2295-2320	0.333333	52.6
ndhI-rps15_p1	ndhI-rps15	1	TTGACCAATGTACCTTGCCG	AAACGTCAACGACTACTGGG	20	20	50.0	50.0	58.5	57.9	5.4	9.9	0.615256	116645	118284	1322-1802	0.500000	64.7
ndhI-rps15_p2	ndhI-rps15	2	CGTTCTGAGGCGATCCATTT	AAACGTCAACGACTACTGGG	20	20	50.0	50.0	58.3	57.9	0.0	9.9	0.487829	116645	118284	1884-1885	0.333333	49.5
ndhI-rps15_p3	ndhI-rps15	3	AAAAGGATTCGTGCATCCCT	AAACGTCAACGACTACTGGG	20	20	45.0	50.0	57.5	57.9	0.3	9.9	0.658940	116645	118284	2437-2445	0.333333	48.4
ndhI-rps15_p4	ndhI-rps15	4	CGTTCTGAGGCGATCCATTT	GAGGCTAAATGGGCTGGAAT	20	20	50.0	50.0	58.3	57.6	0.0	0.0	0.715005	116645	118284	2133-2134	0.333333	47.7
ndhI-rps15_p5	ndhI-rps15	5	TTGACCAATGTACCTTGCCG	GAGGCTAAATGGGCTGGAAT	20	20	50.0	50.0	58.5	57.6	5.4	0.0	0.842432	116645	118284	2050-2051	0.333333	46.7
ndhB_p1	ndhB	1	ATGGCAGAGGCAAATAGAGC	GTCTCGCGTGTCTACCATTT	20	20	50.0	50.0	58.0	58.0	0.0	5.8	0.029376	135802	136725	1684-2344	1.000000	91.8
ndhB_p2	ndhB	2	ATGGCAGAGGCAAATAGAGC	CTCGCGTGTCTACCATTTCA	20	20	50.0	50.0	58.0	58.0	0.0	5.8	0.030746	135802	136725	1682-2342	1.000000	91.8
ndhB_p3	ndhB	3	ATGGCAGAGGCAAATAGAGC	AGATGGAATGGAACGACGTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.031691	135802	136725	1346-2120	1.000000	91.7
ndhB_p4	ndhB	4	TCCGAATTAGTGGATGCTGC	GTCTCGCGTGTCTACCATTT	20	20	50.0	50.0	58.0	58.0	0.0	5.8	0.054271	135802	136725	1750-2410	1.000000	91.6
ndhB_p5	ndhB	5	ATGGCAGAGGCAAATAGAGC	GAGTCGGACATCCAATTGCT	20	20	50.0	50.0	58.0	58.0	0.0	11.3	0.051384	135802	136725	1540-2163	0.833333	84.9