primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	TACCGTTGAGTTAGCAACCC	AGAGAATTTCAAGTTTCTTTGGA	20	23	50.0	30.4	57.5	54.4	0.0	0.0	7.075836	4297	4731	491-538	1.000000	47.9
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAAGCCGAGTACTCTACCGT	AGAGAATTTCAAGTTTCTTTGGA	20	23	50.0	30.4	58.2	54.4	20.3	0.0	6.783498	4297	4731	505-552	1.000000	47.7
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAGCCGAGTACTCTACCGTT	AGAGAATTTCAAGTTTCTTTGGA	20	23	50.0	30.4	58.2	54.4	20.3	0.0	6.783498	4297	4731	504-551	1.000000	47.7
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	AGAGAATTTCAAGTTTCTTTGGAT	20	24	50.0	29.2	58.2	54.9	20.3	0.0	7.231921	4297	4731	504-551	1.000000	47.7
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AGGATCAGTCGCGGTCTTAT	AGAGAATTTCAAGTTTCTTTGGA	20	23	50.0	30.4	58.3	54.4	6.1	0.0	6.921452	4297	4731	590-637	1.000000	46.6
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.540425	7530	8452	875-1098	1.000000	61.7
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.541813	7530	8452	874-1097	1.000000	61.7
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	7530	8452	878-1101	1.000000	60.6
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	7530	8452	877-1100	1.000000	60.6
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.674529	7530	8452	876-1099	1.000000	60.6
rpoC1_p1	rpoC1	1	AGTATAGCATGAGTTAGCTAGC	TCCAGATGAATAGGAAGGAAGT	22	22	40.9	40.9	55.0	56.0	6.6	0.0	9.051104	20234	23036	2877-2883	0.666667	27.4
rpoC1_p2	rpoC1	2	AGTATAGCATGAGTTAGCTAGC	AGGAAGGAAGTTTGATCTCAATGA	22	24	40.9	37.5	55.0	57.9	6.6	0.0	9.150328	20234	23036	2866-2872	0.666667	27.4
rpoC1_p3	rpoC1	3	AGTATAGCATGAGTTAGCTAGC	TCCAGATGAATAGGAAGGAAGTT	22	23	40.9	39.1	55.0	56.7	6.6	0.0	9.325299	20234	23036	2877-2883	0.666667	27.4
rpoC1_p4	rpoC1	4	AGTATAGCATGAGTTAGCTAGC	TTCCAGATGAATAGGAAGGAAGT	22	23	40.9	39.1	55.0	56.7	6.6	0.0	9.325299	20234	23036	2878-2884	0.666667	27.4
rpoC1_p5	rpoC1	5	AGTATAGCATGAGTTAGCTAGC	AGGAAGGAAGTTTGATCTCA	22	20	40.9	40.0	55.0	53.5	6.6	0.0	9.470659	20234	23036	2866-2872	0.666667	27.4
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTGGATGAAATTTGGCGGC	AGCCCAAGCGAGACTTACTA	20	20	45.0	50.0	57.9	58.2	0.0	0.0	0.243200	27691	28355	760-788	1.000000	84.9
trnC-GCA-petN_p2	trnC-GCA-petN	2	CCAGTTCGAATCTGGGTGTC	AGCCCAAGCGAGACTTACTA	20	20	55.0	50.0	58.3	58.2	26.3	0.0	0.424495	27691	28355	699-727	1.000000	84.0
trnC-GCA-petN_p3	trnC-GCA-petN	3	GATGAAATTTGGCGGCATGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.3	0.0	0.577214	27691	28355	756-784	1.000000	82.2
trnC-GCA-petN_p4	trnC-GCA-petN	4	GGATGAAATTTGGCGGCATG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.3	0.0	0.577214	27691	28355	757-785	1.000000	82.2
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTTGGATGAAATTTGGCGGC	CCCACTCCTTCCCCATACTA	20	20	45.0	55.0	57.9	57.5	0.0	0.0	0.581380	27691	28355	817-845	1.000000	81.7
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	ACGTCAAAACAGTCAGCCAA	20	20	50.0	45.0	58.2	58.3	0.0	0.0	0.406116	28446	29233	848-936	1.000000	84.9
petN-psbM_p2	petN-psbM	2	TAGTATGGGGAAGGAGTGGG	ACGTCAAAACAGTCAGCCAA	20	20	55.0	45.0	57.5	58.3	0.0	0.0	0.744296	28446	29233	791-879	1.000000	82.4
petN-psbM_p3	petN-psbM	3	TAGTAAGTCTCGCTTGGGCT	TACGTCAAAACAGTCAGCCA	20	20	50.0	45.0	58.2	57.4	0.0	0.0	0.757186	28446	29233	849-937	1.000000	82.1
petN-psbM_p4	petN-psbM	4	TAGTAAGTCTCGCTTGGGCT	CGTCAAAACAGTCAGCCAAA	20	20	50.0	45.0	58.2	57.2	0.0	0.0	0.993556	28446	29233	847-935	1.000000	80.2
petN-psbM_p5	petN-psbM	5	TAGTATGGGGAAGGAGTGGG	TACGTCAAAACAGTCAGCCA	20	20	55.0	45.0	57.5	57.4	0.0	0.0	1.095365	28446	29233	792-880	1.000000	79.6
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	CAGCGCAGTAGCAATAAACG	TTCGTCGCTCCATGAAGAAG	20	20	50.0	50.0	57.9	58.0	11.5	0.0	0.080863	29339	30307	1306-1337	0.666667	70.4
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	CAGCGCAGTAGCAATAAACG	CCATGCATATGGCTGGGATT	20	20	50.0	50.0	57.9	58.1	11.5	0.0	0.144134	29339	30307	1073-1096	0.666667	69.9
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	ATGAATCTTTTCGAGGGGCC	TTCGTCGCTCCATGAAGAAG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.062301	29339	30307	1851-1851	0.333333	52.8
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	ATGAATCTTTTCGAGGGGCC	CCATGCATATGGCTGGGATT	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.125571	29339	30307	1610-1610	0.333333	52.3
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	ATGAATCTTTTCGAGGGGCC	AGTTCAATCGGTCAGAGCAC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.144647	29339	30307	1588-1588	0.333333	52.2
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.175803	30857	32179	820-1427	1.000000	88.9
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.233900	30857	32179	799-1406	1.000000	88.6
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CGTTGCCTCCTTGAAAGAGA	TGGCGTTACTCTACCACTGA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.256054	30857	32179	796-1403	1.000000	88.4
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	30857	32179	798-1405	1.000000	88.1
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	20	20	50.0	55.0	57.8	58.2	0.0	0.0	0.320736	30857	32179	817-1424	1.000000	87.8
atpB-rbcL_p1	atpB-rbcL	1	AGGGGGCTAAAAGATCCACT	GTCCACACAGTTGTCCATGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.045602	52842	53827	1599-1703	1.000000	87.2
atpB-rbcL_p2	atpB-rbcL	2	TTTTTCCTCCACGGCGATAG	GTCCACACAGTTGTCCATGT	20	20	50.0	50.0	58.0	58.0	16.6	0.0	0.051223	52842	53827	1617-1721	1.000000	87.2
atpB-rbcL_p3	atpB-rbcL	3	AGGGGGCTAAAAGATCCACT	AGAAACGATCTCTCCAACGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.015251	52842	53827	2042-2146	1.000000	86.9
atpB-rbcL_p4	atpB-rbcL	4	ATCTTGCCCGGTGGAAAAAT	AGAAACGATCTCTCCAACGC	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.065140	52842	53827	1677-1781	1.000000	86.9
atpB-rbcL_p5	atpB-rbcL	5	TTTTTCCTCCACGGCGATAG	AGAAACGATCTCTCCAACGC	20	20	50.0	50.0	58.0	58.0	16.6	0.0	0.020873	52842	53827	2060-2164	1.000000	86.8
psaI-ycf4_p1	psaI-ycf4	1	GCAATCGCAATGGCTTCTTT	CAGTACGGGTCAAGGGAATG	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.024605	58300	58724	929-951	1.000000	86.1
psaI-ycf4_p2	psaI-ycf4	2	GCAATCGCAATGGCTTCTTT	ATCAGTACGGGTCAAGGGAA	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.053445	58300	58724	931-953	1.000000	85.9
psaI-ycf4_p3	psaI-ycf4	3	GCAATCGCAATGGCTTCTTT	TCAGTACGGGTCAAGGGAAT	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.053445	58300	58724	930-952	1.000000	85.9
psaI-ycf4_p4	psaI-ycf4	4	GCAATCGCAATGGCTTCTTT	CTCCTTGGCCCCTTATTTCC	20	20	45.0	55.0	58.0	57.9	0.0	0.0	0.073681	58300	58724	908-930	1.000000	85.8
psaI-ycf4_p5	psaI-ycf4	5	GCAATCGCAATGGCTTCTTT	AGGTACGCGCAAGAAATAGG	20	20	45.0	50.0	58.0	58.1	0.0	5.7	0.064497	58300	58724	997-1019	1.000000	85.4
petA-psbJ_p1	petA-psbJ	1	GGCATCTGTGATTTTGGCAC	CGGGTTGGGTTCATCTCTTT	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.200802	61936	62993	1085-1166	1.000000	86.7
petA-psbJ_p2	petA-psbJ	2	TGGCATCTGTGATTTTGGCA	CGGGTTGGGTTCATCTCTTT	20	20	45.0	50.0	58.4	57.8	0.0	0.0	0.570150	61936	62993	1086-1167	1.000000	83.8
petA-psbJ_p3	petA-psbJ	3	GGCATCTGTGATTTTGGCAC	TATTCCGGGTTGGGTTCATC	20	20	50.0	50.0	58.0	57.3	0.0	0.0	0.721854	61936	62993	1090-1171	1.000000	82.6
petA-psbJ_p4	petA-psbJ	4	GGCATCTGTGATTTTGGCAC	CTATTCCGGGTTGGGTTCAT	20	20	50.0	50.0	58.0	57.3	0.0	0.0	0.729457	61936	62993	1091-1172	1.000000	82.5
petA-psbJ_p5	petA-psbJ	5	GCATCTGTGATTTTGGCACA	CGGGTTGGGTTCATCTCTTT	20	20	45.0	50.0	57.3	57.8	0.0	0.0	0.929062	61936	62993	1084-1165	1.000000	80.9
psbE-petL_p1	psbE-petL	1	CAAAAGAACGTTCTCCCGTG	GCTGGGGTTATAGTTGAAGCA	20	21	50.0	47.6	57.3	58.0	29.8	0.0	1.663948	63758	64994	1314-1327	1.000000	75.0
psbE-petL_p2	psbE-petL	2	CAAAAGAACGTTCTCCCGTG	GGGTTATAGTTGAAGCAGCCA	20	21	50.0	47.6	57.3	58.0	29.8	0.0	1.663948	63758	64994	1310-1323	1.000000	75.0
psbE-petL_p3	psbE-petL	3	CAAAAGAACGTTCTCCCGTG	TGGGGTTATAGTTGAAGCAGC	20	21	50.0	47.6	57.3	58.0	29.8	0.0	1.663948	63758	64994	1312-1325	1.000000	75.0
psbE-petL_p4	psbE-petL	4	CAGCAAAAGAACGTTCTCCC	GGGTTATAGTTGAAGCAGCCA	20	21	50.0	47.6	57.3	58.0	29.8	0.0	1.689033	63758	64994	1313-1326	1.000000	74.8
psbE-petL_p5	psbE-petL	5	CAGCAAAAGAACGTTCTCCC	TGGGGTTATAGTTGAAGCAGC	20	21	50.0	47.6	57.3	58.0	29.8	0.0	1.689033	63758	64994	1315-1328	1.000000	74.8
trnW-CCA-trnP-UGG_p1	trnW-CCA-trnP-UGG	1	GCGTTCTACCGAACTGAACT	GTCACGGGTTCAAATCCTGT	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.169530	65587	65752	207-222	1.000000	86.7
trnW-CCA-trnP-UGG_p2	trnW-CCA-trnP-UGG	2	GCGTTCTACCGAACTGAACT	CACGGGTTCAAATCCTGTCA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.170864	65587	65752	205-220	1.000000	86.7
trnW-CCA-trnP-UGG_p3	trnW-CCA-trnP-UGG	3	GCGTTCTACCGAACTGAACT	TGTCACGGGTTCAAATCCTG	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.170864	65587	65752	208-223	1.000000	86.7
trnW-CCA-trnP-UGG_p4	trnW-CCA-trnP-UGG	4	GCGTTCTACCGAACTGAACT	GGGTACAAAATGTCACGGGT	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.161494	65587	65752	218-233	1.000000	86.6
trnW-CCA-trnP-UGG_p5	trnW-CCA-trnP-UGG	5	GCGTTCTACCGAACTGAACT	TGGGTACAAAATGTCACGGG	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.162838	65587	65752	219-234	1.000000	86.5
clpP-psbB_p1	clpP-psbB	1	CGCACTATACGTCAACCCAA	CGGATCAAGAACAGGGTCAG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.016687	71357	71995	877-918	1.000000	87.3
clpP-psbB_p2	clpP-psbB	2	CGCACTATACGTCAACCCAA	ACTCCAAATGTCCGGATTCG	20	20	50.0	50.0	58.0	58.0	0.0	7.8	0.025127	71357	71995	991-1032	1.000000	86.5
clpP-psbB_p3	clpP-psbB	3	CGCACTATACGTCAACCCAA	CTCCAAATGTCCGGATTCGT	20	20	50.0	50.0	58.0	58.0	0.0	7.8	0.025127	71357	71995	990-1031	1.000000	86.5
clpP-psbB_p4	clpP-psbB	4	CGCACTATACGTCAACCCAA	ACACCCATATTCCAGGACCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.021142	71357	71995	1220-1261	1.000000	85.9
clpP-psbB_p5	clpP-psbB	5	CGCACTATACGTCAACCCAA	TAGACCCGCTAATATGCCCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.019307	71357	71995	1357-1398	1.000000	85.5
psbB-psbT_p1	psbB-psbT	1	TGGAGATCCAACTACAAGGAGA	GGAATGTATAAACCAATGCTTCCA	22	24	45.5	37.5	57.9	57.5	0.0	0.0	6.525125	73523	74116	447-668	1.000000	48.9
psbB-psbT_p2	psbB-psbT	2	TGGAGATCCAACTACAAGGAGA	AGGAATGTATAAACCAATGCTTCC	22	24	45.5	37.5	57.9	57.3	0.0	0.0	6.776478	73523	74116	448-669	1.000000	48.9
psbB-psbT_p3	psbB-psbT	3	GGAGATCCAACTACAAGGAGAC	GGAATGTATAAACCAATGCTTCCA	22	24	50.0	37.5	57.6	57.5	0.0	0.0	6.858434	73523	74116	446-667	1.000000	48.9
psbB-psbT_p4	psbB-psbT	4	TGGAGATCCAACTACAAGGAGA	AGTCGAAACTAAGAGGAATGT	22	21	45.5	38.1	57.9	54.3	0.0	0.0	6.741696	73523	74116	461-682	1.000000	48.8
psbB-psbT_p5	psbB-psbT	5	TGGAGATCCAACTACAAGGAGA	AAGTCGAAACTAAGAGGAATGT	22	22	45.5	36.4	57.9	55.1	0.0	0.0	6.924556	73523	74116	462-683	1.000000	48.8
ndhB_p1	ndhB	1	TCGAATGTGATAAGTTCCGGT	TCGTAGATTGGATGCAGTTACT	21	22	42.9	40.9	57.1	57.0	0.0	0.0	4.882490	95678	97423	1857-1857	0.333333	14.3
ndhB_p2	ndhB	2	TCTCGAATGTGATAAGTTCCGG	TCGTAGATTGGATGCAGTTACT	22	22	45.5	40.9	58.0	57.0	0.0	0.0	5.044183	95678	97423	1859-1859	0.333333	13.3
ndhB_p3	ndhB	3	TCGAATGTGATAAGTTCCGGT	TTCGTAGATTGGATGCAGTT	21	20	42.9	40.0	57.1	54.8	0.0	0.0	5.108717	95678	97423	1858-1858	0.333333	13.3
ndhB_p4	ndhB	4	TCGAATGTGATAAGTTCCGGT	TTTCGTAGATTGGATGCAGT	21	20	42.9	40.0	57.1	54.8	0.0	0.0	5.108717	95678	97423	1859-1859	0.333333	13.3
ndhB_p5	ndhB	5	TCGAATGTGATAAGTTCCGGT	TTCGTAGATTGGATGCAGTTACT	21	23	42.9	39.1	57.1	57.7	0.0	0.0	5.219055	95678	97423	1858-1858	0.333333	13.3
trnN-GUU-rpl32_p1	trnN-GUU-rpl32	1	AGTCAGTTAACAGCCAACCG	TGACATAGAAGTGCGTTTTT	20	20	50.0	35.0	58.1	53.5	8.6	0.0	4.657162	109260	110522	1339-1477	1.000000	54.7
trnN-GUU-rpl32_p2	trnN-GUU-rpl32	2	AGTCAGTTAACAGCCAACCG	TTGACATAGAAGTGCGTTTT	20	20	50.0	35.0	58.1	53.5	8.6	0.0	4.657162	109260	110522	1340-1478	1.000000	54.7
trnN-GUU-rpl32_p3	trnN-GUU-rpl32	3	AGTCAGTTAACAGCCAACCG	TTTGACATAGAAGTGCGTTT	20	20	50.0	35.0	58.1	53.5	8.6	0.0	4.657162	109260	110522	1341-1479	1.000000	54.7
trnN-GUU-rpl32_p4	trnN-GUU-rpl32	4	AGTCAGTTAACAGCCAACCG	TTTTGACATAGAAGTGCGTT	20	20	50.0	35.0	58.1	53.5	8.6	0.0	4.657162	109260	110522	1342-1480	1.000000	54.7
trnN-GUU-rpl32_p5	trnN-GUU-rpl32	5	AGTCAGTTAACAGCCAACCG	TTTTTGACATAGAAGTGCGT	20	20	50.0	35.0	58.1	53.5	8.6	0.0	4.657162	109260	110522	1343-1481	1.000000	54.7
ccsA-psaC_p1	ccsA-psaC	1	ATGCAGCTCTTTTATGCGGA	CGGATTTCTTGAGTGTCCGT	20	20	45.0	50.0	58.0	57.9	0.0	4.1	0.122972	112421	112993	1158-1168	1.000000	84.0
ccsA-psaC_p2	ccsA-psaC	2	TGCAGCTCTTTTATGCGGAT	CGGATTTCTTGAGTGTCCGT	20	20	45.0	50.0	58.0	57.9	0.0	4.1	0.122972	112421	112993	1157-1167	1.000000	84.0
ccsA-psaC_p3	ccsA-psaC	3	TGCAGCTCTTTTATGCGGAT	GTACCCAATGTGTTCGAGCT	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.137448	112421	112993	1295-1305	1.000000	83.5
ccsA-psaC_p4	ccsA-psaC	4	ATGCAGCTCTTTTATGCGGA	GTACCCAATGTGTTCGAGCT	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.137448	112421	112993	1296-1306	1.000000	83.4
ccsA-psaC_p5	ccsA-psaC	5	TTTTACCCAAGGTTTCGCCA	CGGATTTCTTGAGTGTCCGT	20	20	45.0	50.0	57.9	57.9	0.0	4.1	0.149120	112421	112993	1277-1287	0.666667	68.2