primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score trnH(GUG)-psbA_p1 trnH(GUG)-psbA 1 TTAGTTATGGGCGAACGACG CCGTGCTAACCTTGGTATGG 20 20 50.0 55.0 58.1 58.3 0.0 0.0 0.424304 94 538 622-625 1.000000 77.4 trnH(GUG)-psbA_p2 trnH(GUG)-psbA 2 CTGCCTTAATCCACTTGGCT CCGTGCTAACCTTGGTATGG 20 20 50.0 55.0 57.9 58.3 0.0 0.0 0.471094 94 538 567-570 1.000000 77.1 trnH(GUG)-psbA_p3 trnH(GUG)-psbA 3 TTAGTTATGGGCGAACGACG ACCGTGCTAACCTTGGTATG 20 20 50.0 50.0 58.1 57.6 0.0 0.0 0.484588 94 538 623-626 1.000000 76.9 trnH(GUG)-psbA_p4 trnH(GUG)-psbA 4 ACTGCCTTAATCCACTTGGC CCGTGCTAACCTTGGTATGG 20 20 50.0 55.0 58.2 58.3 0.0 0.0 0.496882 94 538 568-571 1.000000 76.9 trnH(GUG)-psbA_p5 trnH(GUG)-psbA 5 CTGCCTTAATCCACTTGGCT ACCGTGCTAACCTTGGTATG 20 20 50.0 50.0 57.9 57.6 0.0 0.0 0.531379 94 538 568-571 1.000000 76.6 trnS(GCU)-trnG(GCC)_p1 trnS(GCU)-trnG(GCC) 1 CAATCCGACGCTTTAGTCCA AGACCGAAAGACCCTTTAACT 20 21 50.0 42.9 58.0 56.6 0.0 0.0 2.425660 9039 9742 804-811 1.000000 61.5 trnS(GCU)-trnG(GCC)_p2 trnS(GCU)-trnG(GCC) 2 AATCCGACGCTTTAGTCCAC AGACCGAAAGACCCTTTAACT 20 21 50.0 42.9 58.0 56.6 0.0 0.0 2.427048 9039 9742 803-810 1.000000 61.5 trnS(GCU)-trnG(GCC)_p3 trnS(GCU)-trnG(GCC) 3 ATCAACGGAACCGGAAAGAG AGACCGAAAGACCCTTTAACT 20 21 50.0 42.9 57.9 56.6 9.5 0.0 2.515840 9039 9742 868-875 1.000000 60.7 trnS(GCU)-trnG(GCC)_p4 trnS(GCU)-trnG(GCC) 4 CATCAACGGAACCGGAAAGA AGACCGAAAGACCCTTTAACT 20 21 50.0 42.9 58.2 56.6 9.5 0.0 2.617743 9039 9742 869-876 1.000000 59.9 trnS(GCU)-trnG(GCC)_p5 trnS(GCU)-trnG(GCC) 5 TCATCAACGGAACCGGAAAG AGACCGAAAGACCCTTTAACT 20 21 50.0 42.9 58.2 56.6 9.5 0.0 2.617743 9039 9742 870-877 1.000000 59.9 atpF_p1 atpF 1 GGCTCGAATGGTTACCATGA AGGCAAAGTGATACAAAAAGAACT 20 24 50.0 33.3 57.7 57.3 18.5 0.0 5.040930 12425 13687 1392-1405 1.000000 40.7 atpF_p2 atpF 2 GGCTCGAATGGTTACCATGA GGCAAAGTGATACAAAAAGAACTC 20 24 50.0 37.5 57.7 57.0 18.5 0.0 5.318758 12425 13687 1391-1404 1.000000 40.7 atpF_p3 atpF 3 ACTAATTTCGTCGGCTCGAA AGGCAAAGTGATACAAAAAGAACT 20 24 45.0 33.3 57.4 57.3 9.6 0.0 5.357485 12425 13687 1404-1417 1.000000 40.7 atpF_p4 atpF 4 GGCTCGAATGGTTACCATGA AGGCAAAGTGATACAAAAAGAAC 20 23 50.0 34.8 57.7 55.9 18.5 0.0 5.391963 12425 13687 1392-1405 1.000000 40.7 atpF_p5 atpF 5 GGCTCGAATGGTTACCATGA GGCAAAGTGATACAAAAAGAACT 20 23 50.0 34.8 57.7 55.9 18.5 0.0 5.391963 12425 13687 1391-1404 1.000000 40.7 atpH-atpI_p1 atpH-atpI 1 AACAGAAGCGGCAGAAATCA TCCCTGTCATGTTCCTTGGA 20 20 45.0 50.0 58.1 58.3 1.4 0.0 0.362752 14299 15367 1203-1203 1.000000 77.4 atpH-atpI_p2 atpH-atpI 2 TACCCTCTACAGCTTGACCC TCCCTGTCATGTTCCTTGGA 20 20 55.0 50.0 58.1 58.3 0.0 0.0 0.396990 14299 15367 1288-1288 1.000000 77.1 atpH-atpI_p3 atpH-atpI 3 GCGATACCCTCTACAGCTTG TCCCTGTCATGTTCCTTGGA 20 20 55.0 50.0 57.9 58.3 0.0 0.0 0.400507 14299 15367 1292-1292 1.000000 77.0 atpH-atpI_p4 atpH-atpI 4 CAGCAGCAATAACAGAAGCG TCCCTGTCATGTTCCTTGGA 20 20 50.0 50.0 57.8 58.3 0.0 0.0 0.428635 14299 15367 1213-1213 1.000000 76.8 atpH-atpI_p5 atpH-atpI 5 GCCAATCCAGCAGCAATAAC TCCCTGTCATGTTCCTTGGA 20 20 50.0 50.0 57.8 58.3 0.0 0.0 0.473359 14299 15367 1220-1220 1.000000 76.5 rpoC1_p1 rpoC1 1 CGGAACTATGGGCCTAGTTG TTCGATTGCTCGTTCGAGAA 20 20 55.0 45.0 57.8 57.7 24.0 23.5 0.581811 21584 24416 2995-2997 1.000000 75.4 rpoC1_p2 rpoC1 2 CGGAACTATGGGCCTAGTTG TTTCGATTGCTCGTTCGAGA 20 20 55.0 45.0 57.8 57.7 24.0 23.5 0.581811 21584 24416 2996-2998 1.000000 75.4 rpoC1_p3 rpoC1 3 ATTCCGGAACTATGGGCCTA TCGATTGCTCGTTCGAGAAT 20 20 50.0 45.0 57.9 57.4 21.6 23.5 0.674012 21584 24416 2998-3000 1.000000 74.7 rpoC1_p4 rpoC1 4 CGGAACTATGGGCCTAGTTG TCGATTGCTCGTTCGAGAAT 20 20 55.0 45.0 57.8 57.4 24.0 23.5 0.802227 21584 24416 2994-2996 1.000000 73.7 rpoC1_p5 rpoC1 5 TTCCGGAACTATGGGCCTAG TTCGATTGCTCGTTCGAGAA 20 20 55.0 45.0 58.6 57.7 14.3 23.5 0.920888 21584 24416 2998-3000 1.000000 72.7 petN-psbM_p1 petN-psbM 1 ACTCGTAGTGTGGGGAAGAA GCGACTGCACTATTCATTCT 20 20 50.0 45.0 58.0 55.9 0.0 0.0 2.059024 29783 30948 1275-1275 1.000000 64.0 petN-psbM_p2 petN-psbM 2 TCGTAGTGTGGGGAAGAAGT GCGACTGCACTATTCATTCT 20 20 50.0 45.0 58.0 55.9 0.0 0.0 2.059024 29783 30948 1273-1273 1.000000 64.0 petN-psbM_p3 petN-psbM 3 CGTAGTGTGGGGAAGAAGTG GCGACTGCACTATTCATTCT 20 20 55.0 45.0 57.9 55.9 0.0 0.0 2.133939 29783 30948 1272-1272 1.000000 63.4 petN-psbM_p4 petN-psbM 4 TAGTGTGGGGAAGAAGTGGA GCGACTGCACTATTCATTCT 20 20 50.0 45.0 57.6 55.9 0.0 0.0 2.462717 29783 30948 1270-1270 1.000000 60.8 petN-psbM_p5 petN-psbM 5 CCTGGGCTGCTTTAATGGTA GCGACTGCACTATTCATTCT 20 20 50.0 45.0 57.6 55.9 0.0 0.0 2.489026 29783 30948 1315-1315 1.000000 60.6 trnM(CAU)-psbD_p1 trnM(CAU)-psbD 1 GTGGTAGAGTAACGCCATGG GGACCAGCCTACAAAAACGA 20 20 55.0 50.0 58.1 58.1 7.6 0.0 0.177181 32955 34270 1475-1476 1.000000 78.9 trnM(CAU)-psbD_p2 trnM(CAU)-psbD 2 TCAGTGGTAGAGTAACGCCA GGACCAGCCTACAAAAACGA 20 20 50.0 50.0 58.1 58.1 0.0 0.0 0.199336 32955 34270 1478-1479 1.000000 78.7 trnM(CAU)-psbD_p3 trnM(CAU)-psbD 3 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 20 20 55.0 45.0 58.1 58.2 7.6 0.0 0.310137 32955 34270 1465-1466 1.000000 77.8 trnM(CAU)-psbD_p4 trnM(CAU)-psbD 4 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 20 20 50.0 45.0 58.1 58.2 0.0 0.0 0.332292 32955 34270 1468-1469 1.000000 77.6 trnM(CAU)-psbD_p5 trnM(CAU)-psbD 5 GGCGTAAGTCATCGGTTCAA GGACCAGCCTACAAAAACGA 20 20 50.0 50.0 58.3 58.1 0.0 0.0 0.395920 32955 34270 1452-1453 1.000000 77.1 ndhC-trnM(CAU)_p1 ndhC-trnM(CAU) 1 GGGGCTAAAATCCCGGAAAT CCCGCCGTATGAAAGCAATA 20 20 50.0 50.0 57.9 58.4 0.7 0.0 0.493466 52116 53907 1934-1934 1.000000 76.2 ndhC-trnM(CAU)_p2 ndhC-trnM(CAU) 2 GGGGCTAAAATCCCGGAAAT AGGTACCATTGACTCCGGTA 20 20 50.0 50.0 57.9 57.5 0.7 15.9 0.619773 52116 53907 1995-1995 1.000000 75.2 ndhC-trnM(CAU)_p3 ndhC-trnM(CAU) 3 GGGGCTAAAATCCCGGAAAT TAGGTACCATTGACTCCGGT 20 20 50.0 50.0 57.9 57.5 0.7 15.9 0.619773 52116 53907 1996-1996 1.000000 75.2 ndhC-trnM(CAU)_p4 ndhC-trnM(CAU) 4 GGGGCTAAAATCCCGGAAAT CCGCCGTATGAAAGCAATAC 20 20 50.0 50.0 57.9 57.3 0.7 0.0 0.745419 52116 53907 1933-1933 1.000000 74.2 ndhC-trnM(CAU)_p5 ndhC-trnM(CAU) 5 TTCTCCGGCCCTTTCCTAAT CCCGCCGTATGAAAGCAATA 20 20 50.0 50.0 58.4 58.4 0.0 0.0 0.819557 52116 53907 1955-1955 1.000000 73.6 atpB-rbcL_p1 atpB-rbcL 1 ATACGGCCCAGGTTTTGTTT AACACCCGCTTTGAATCCAA 20 20 45.0 45.0 58.0 58.3 0.0 0.0 0.305431 56081 56871 904-910 1.000000 79.4 atpB-rbcL_p2 atpB-rbcL 2 GGAAACCCCAGAACCAGAAG AACACCCGCTTTGAATCCAA 20 20 55.0 45.0 58.1 58.3 0.0 0.0 0.390437 56081 56871 875-881 1.000000 78.8 atpB-rbcL_p3 atpB-rbcL 3 GGCGGAAAGGCTACATCTAG AACACCCGCTTTGAATCCAA 20 20 55.0 45.0 57.8 58.3 0.0 0.0 0.457837 56081 56871 946-952 1.000000 78.1 atpB-rbcL_p4 atpB-rbcL 4 TACGGCCCAGGTTTTGTTTT AACACCCGCTTTGAATCCAA 20 20 45.0 45.0 58.2 58.3 0.0 0.0 0.523554 56081 56871 903-909 1.000000 77.7 atpB-rbcL_p5 atpB-rbcL 5 GGACCGATGATTTGGACGAT AACACCCGCTTTGAATCCAA 20 20 50.0 45.0 57.8 58.3 0.0 0.0 0.541987 56081 56871 922-928 1.000000 77.5 accD-psaI_p1 accD-psaI 1 GCTCCACGCTTTCTTTCCTT CCATTGCAATTGCCGGAAAT 20 20 50.0 45.0 58.8 58.0 0.0 26.9 0.787656 60453 61153 799-799 1.000000 74.0 accD-psaI_p2 accD-psaI 2 GCTCCACGCTTTCTTTCCTT AAGAAGCCATTGCAATTGCC 20 20 50.0 45.0 58.8 57.9 0.0 26.8 0.864190 60453 61153 805-805 1.000000 73.4 accD-psaI_p3 accD-psaI 3 GCTCCACGCTTTCTTTCCT CCATTGCAATTGCCGGAAAT 19 20 52.6 45.0 58.1 58.0 0.0 26.9 1.092920 60453 61153 799-799 1.000000 71.6 accD-psaI_p4 accD-psaI 4 TCCACGCTTTCTTTCCTTTGA CCATTGCAATTGCCGGAAAT 21 20 42.9 45.0 58.1 58.0 0.0 26.9 1.101279 60453 61153 797-797 1.000000 71.5 accD-psaI_p5 accD-psaI 5 GCTCCACGCTTTCTTTCCT AAGAAGCCATTGCAATTGCC 19 20 52.6 45.0 58.1 57.9 0.0 26.8 1.169454 60453 61153 805-805 1.000000 70.9 ycf4_p1 ycf4 1 TGGGGTATTCTCTCAATTCCA TTCTTGCATTTTGCCCTCCT 21 20 42.9 45.0 56.0 58.0 0.0 0.0 2.987267 61708 62262 658-658 1.000000 56.3 ycf4_p2 ycf4 2 TGGGGTATTCTCTCAATTCCAA TTCTTGCATTTTGCCCTCCT 22 20 40.9 45.0 56.8 58.0 0.0 0.0 3.231486 61708 62262 658-658 1.000000 54.4 ycf4_p3 ycf4 3 TTGGGGTATTCTCTCAATTCCA TTCTTGCATTTTGCCCTCCT 22 20 40.9 45.0 56.8 58.0 0.0 0.0 3.231486 61708 62262 659-659 1.000000 54.4 ycf4_p4 ycf4 4 AGTTGATGAGAGTTACTTCGGA TTCTTGCATTTTGCCCTCCT 22 20 40.9 45.0 56.6 58.0 0.0 0.0 3.420112 61708 62262 708-709 1.000000 53.0 ycf4_p5 ycf4 5 TGATGAGAGTTACTTCGGAAACA TTCTTGCATTTTGCCCTCCT 23 20 39.1 45.0 57.5 58.0 0.0 0.0 3.476540 61708 62262 705-706 1.000000 52.5 psbE-petL_p1 psbE-petL 1 CAACCCGCAATGAATAGGGA GTCGTATCTTGCTCAGACCA 20 20 50.0 50.0 57.9 57.1 6.4 0.0 0.992787 66760 67940 1372-1373 1.000000 72.2 psbE-petL_p2 psbE-petL 2 ACTGACGAATAACCAACCCG GTCGTATCTTGCTCAGACCA 20 20 50.0 50.0 57.9 57.1 0.0 0.0 1.030402 66760 67940 1385-1386 1.000000 71.9 psbE-petL_p3 psbE-petL 3 AGAACGTTCTCCTGTGCTTC GTCGTATCTTGCTCAGACCA 20 20 50.0 50.0 57.8 57.1 24.9 0.0 1.097796 66760 67940 1295-1296 1.000000 71.4 psbE-petL_p4 psbE-petL 4 ACGAATAACCAACCCGCAAT GTCGTATCTTGCTCAGACCA 20 20 45.0 50.0 58.2 57.1 0.0 0.0 1.112474 66760 67940 1381-1382 1.000000 71.3 psbE-petL_p5 psbE-petL 5 CGAATAACCAACCCGCAATG GTCGTATCTTGCTCAGACCA 20 20 50.0 50.0 57.8 57.1 0.0 0.0 1.128804 66760 67940 1380-1381 1.000000 71.1 clpP_p1 clpP 1 TGGGCTGGTTTAGATTGATCC TGTTGTTACGTTTCCACATCA 21 21 47.6 38.1 57.7 56.0 0.0 0.0 4.254564 72104 74143 2214-2216 1.000000 46.1 clpP_p2 clpP 2 TGGGCTGGTTTAGATTGATCC TGTTGTTACGTTTCCACATCAA 21 22 47.6 36.4 57.7 56.8 0.0 0.0 4.525932 72104 74143 2214-2216 1.000000 43.9 clpP_p3 clpP 3 GGGCTGGTTTAGATTGATCCT TGTTGTTACGTTTCCACATCA 21 21 47.6 38.1 57.4 56.0 0.0 0.0 4.542162 72104 74143 2213-2215 1.000000 43.8 clpP_p4 clpP 4 TGGGCTGGTTTAGATTGATCC ATGTTGTTACGTTTCCACATCA 21 22 47.6 36.4 57.7 56.6 0.0 0.0 4.734120 72104 74143 2215-2217 1.000000 42.2 clpP_p5 clpP 5 TGGGCTGGTTTAGATTGATCC TGTTACGTTTCCACATCAAAGT 21 22 47.6 36.4 57.7 56.5 0.0 0.0 4.798777 72104 74143 2211-2213 1.000000 41.7 ndhF_p1 ndhF 1 GCAGAATACCGTCACCCATT GAGGTAAGATCCGGTGAATCG 20 21 50.0 52.4 58.0 58.0 0.0 0.0 1.052991 111806 114022 2628-2628 1.000000 71.7 ndhF_p2 ndhF 2 GAGATCCGCCGAAGAAGATC GAGGTAAGATCCGGTGAATCG 20 21 55.0 52.4 57.9 58.0 0.0 0.0 1.073517 111806 114022 2492-2492 1.000000 71.5 ndhF_p3 ndhF 3 CAGGAACAAGAGAGATCCGC GAGGTAAGATCCGGTGAATCG 20 21 55.0 52.4 58.1 58.0 0.0 0.0 1.081357 111806 114022 2503-2503 1.000000 71.5 ndhF_p4 ndhF 4 CGCCGAAGAAGATCCTTCTC ATGCTGTCCCATACCCTTTC 20 20 55.0 50.0 58.2 57.3 9.4 0.0 0.890129 111806 114022 2996-2996 0.333333 46.2 ndhF_p5 ndhF 5 GCCGAAGAAGATCCTTCTCC ATGCTGTCCCATACCCTTTC 20 20 55.0 50.0 57.8 57.3 9.4 0.0 0.969426 111806 114022 2995-2995 0.333333 45.6 ndhF-rpl32_p1 ndhF-rpl32 1 GTTGCCGTCGGAAAAAGAAG TGATGCAGAAGTACGTTTTT 20 20 50.0 35.0 58.0 53.5 17.5 0.0 4.568529 114023 114495 604-607 1.000000 43.7 ndhF-rpl32_p2 ndhF-rpl32 2 GTTGCCGTCGGAAAAAGAAG TTGATGCAGAAGTACGTTTT 20 20 50.0 35.0 58.0 53.5 17.5 0.0 4.568529 114023 114495 605-608 1.000000 43.7 ndhF-rpl32_p3 ndhF-rpl32 3 GTTGCCGTCGGAAAAAGAAG TTTGATGCAGAAGTACGTTT 20 20 50.0 35.0 58.0 53.5 17.5 0.0 4.568529 114023 114495 606-609 1.000000 43.7 ndhF-rpl32_p4 ndhF-rpl32 4 GTTGCCGTCGGAAAAAGAAG TTTTGATGCAGAAGTACGTT 20 20 50.0 35.0 58.0 53.5 17.5 0.0 4.568529 114023 114495 607-610 1.000000 43.7 ndhF-rpl32_p5 ndhF-rpl32 5 GTTGCCGTCGGAAAAAGAAG TTTTTGATGCAGAAGTACGT 20 20 50.0 35.0 58.0 53.5 17.5 0.0 4.568529 114023 114495 608-611 1.000000 43.7 ccsA_p1 ccsA 1 GAGTTCAAGACAAGCCGCTA TCAGTAGCTTTTCGATGGCC 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.104462 115369 116340 1778-1779 1.000000 79.4 ccsA_p2 ccsA 2 GAGTTCAAGACAAGCCGCTA CAGTAGCTTTTCGATGGCCT 20 20 50.0 50.0 57.9 58.0 0.0 3.3 0.110124 115369 116340 1777-1778 1.000000 79.3 ccsA_p3 ccsA 3 TAGACACGCTGCTCTTAGGA TCAGTAGCTTTTCGATGGCC 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.148453 115369 116340 1746-1747 1.000000 79.0 ccsA_p4 ccsA 4 ACAAGCCGCTATGGTGAAAT TCAGTAGCTTTTCGATGGCC 20 20 45.0 50.0 57.9 58.0 0.0 0.0 0.152946 115369 116340 1769-1770 1.000000 79.0 ccsA_p5 ccsA 5 TAGACACGCTGCTCTTAGGA CAGTAGCTTTTCGATGGCCT 20 20 50.0 50.0 57.9 58.0 0.0 3.3 0.154115 115369 116340 1745-1746 1.000000 79.0 ndhE-ndhG_p1 ndhE-ndhG 1 TCGACTTGTGATCAATCCGT GCAATTGCAATAGCTCGTCA 20 20 45.0 45.0 57.0 57.2 0.0 25.4 1.860922 119015 119215 300-300 1.000000 65.6 ndhE-ndhG_p2 ndhE-ndhG 2 TTCGACTTGTGATCAATCCGT GCAATTGCAATAGCTCGTCA 21 20 42.9 45.0 57.7 57.2 0.0 25.4 2.143784 119015 119215 301-301 1.000000 63.4 ndhE-ndhG_p3 ndhE-ndhG 3 TCGACTTGTGATCAATCCGT GCAATTGCAATAGCTCGTCAA 20 21 45.0 42.9 57.0 57.9 0.0 25.4 2.165564 119015 119215 300-300 1.000000 63.2 ndhE-ndhG_p4 ndhE-ndhG 4 TTCGACTTGTGATCAATCCGT GCAATTGCAATAGCTCGTCAA 21 21 42.9 42.9 57.7 57.9 0.0 25.4 2.448426 119015 119215 301-301 1.000000 60.9 ndhE-ndhG_p5 ndhE-ndhG 5 GACACATAAGGGCTCTAACCA GCAATTGCAATAGCTCGTCA 21 20 47.6 45.0 57.1 57.2 0.0 25.4 2.737492 119015 119215 325-325 1.000000 58.6 ycf1_p1 ycf1 1 TCATACCATTCAGGAAGTTGCA GAGGGGGTCAGCATTCAAAA 22 20 40.9 50.0 57.7 58.1 0.0 0.0 2.376231 127910 128029 255-279 1.000000 66.5 ycf1_p2 ycf1 2 ACCATTCAGGAAGTTGCAAT GAGGGGGTCAGCATTCAAAA 20 20 40.0 50.0 55.5 58.1 0.6 0.0 2.571897 127910 128029 251-275 1.000000 64.9 ycf1_p3 ycf1 3 TCAGGAAGTTGCAATAGTAGCA GAGGGGGTCAGCATTCAAAA 22 20 40.9 50.0 57.4 58.1 0.6 0.0 2.628346 127910 128029 246-270 1.000000 64.5 ycf1_p4 ycf1 4 TCATACCATTCAGGAAGTTGCA AGAAATTGGGTAGGGAGGGG 22 20 40.9 55.0 57.7 58.4 0.0 0.0 2.691162 127910 128029 269-293 1.000000 63.9 ycf1_p5 ycf1 5 CATACCATTCAGGAAGTTGCA GAGGGGGTCAGCATTCAAAA 21 20 42.9 50.0 56.2 58.1 0.0 0.0 2.853788 127910 128029 254-278 1.000000 62.7