primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	TTAGTTATGGGCGAACGACG	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.120679	93	634	720-724	1.000000	79.8
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.161600	93	634	658-662	1.000000	79.6
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	TTAGTTATGGGCGAACGACG	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.162727	93	634	673-677	1.000000	79.5
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.165962	93	634	672-676	1.000000	79.5
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	93	634	671-675	1.000000	79.5
matK-trnK-UUU_p1	matK-trnK-UUU	1	AAGTTGCGTTGCTGAGATCT	AACGGTAGAGTACTCGGCTT	20	20	45.0	50.0	57.8	58.2	0.0	17.1	0.344297	3777	4495	796-798	1.000000	77.6
matK-trnK-UUU_p2	matK-trnK-UUU	2	AAGTTGCGTTGCTGAGATCT	ACGGTAGAGTACTCGGCTTT	20	20	45.0	50.0	57.8	58.2	0.0	17.1	0.344297	3777	4495	795-797	1.000000	77.6
matK-trnK-UUU_p3	matK-trnK-UUU	3	TAGGAAGTTGCGTTGCTGAG	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	58.2	58.2	0.0	17.1	0.372652	3777	4495	800-802	1.000000	77.4
matK-trnK-UUU_p4	matK-trnK-UUU	4	TAGGAAGTTGCGTTGCTGAG	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	58.2	58.2	0.0	17.1	0.372652	3777	4495	799-801	1.000000	77.4
matK-trnK-UUU_p5	matK-trnK-UUU	5	AAGTTGCGTTGCTGAGATCT	GGGTTGCTAACTCAACGGTA	20	20	45.0	50.0	57.8	57.5	0.0	0.0	0.636635	3777	4495	809-811	1.000000	75.3
psbK-psbI_p1	psbK-psbI	1	CCAAATTACCCGAGGCCTAC	TTTTATTCTTCACGCCCGGG	20	20	55.0	50.0	58.0	58.5	4.4	19.1	0.581580	8851	9227	605-609	1.000000	76.1
psbK-psbI_p2	psbK-psbI	2	TTAGCCAAATTACCCGAGGC	TTTTATTCTTCACGCCCGGG	20	20	50.0	50.0	58.0	58.5	0.0	19.1	0.591754	8851	9227	609-613	1.000000	76.1
psbK-psbI_p3	psbK-psbI	3	GTTTGGCAAGCTGCTGTAAG	TTTTATTCTTCACGCCCGGG	20	20	50.0	50.0	57.9	58.5	1.4	19.1	0.600846	8851	9227	519-523	1.000000	76.1
psbK-psbI_p4	psbK-psbI	4	TGGCAAGCTGCTGTAAGTTT	TTTTATTCTTCACGCCCGGG	20	20	45.0	50.0	58.3	58.5	1.4	19.1	0.853556	8851	9227	516-520	1.000000	74.1
psbK-psbI_p5	psbK-psbI	5	TTGGCAAGCTGCTGTAAGTT	TTTTATTCTTCACGCCCGGG	20	20	45.0	50.0	58.3	58.5	1.4	19.1	0.853556	8851	9227	517-521	1.000000	74.1
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CCGACGCTTTAATCCACTCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.540425	9578	10457	941-946	1.000000	52.4
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	CCGACGCTTTAATCCACTCA	AGAACGAATCACACTTTTACCAC	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	9578	10457	944-949	1.000000	51.3
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CCGACGCTTTAATCCACTCA	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	9578	10457	943-948	1.000000	51.3
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	AAGATAGCAAGCCATCGACG	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.1	55.5	0.0	0.0	3.677751	9578	10457	1022-1027	1.000000	51.2
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.2	55.5	0.0	0.0	3.687191	9578	10457	999-1004	1.000000	51.1
atpF-atpH_p1	atpF-atpH	1	ATTAAACCCGAAACTCCCGG	CCTGAAGCGGAGGGAAAAAT	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.278890	14525	15051	668-717	1.000000	82.0
atpF-atpH_p2	atpF-atpH	2	ATTAAACCCGAAACTCCCGG	AGCGGAGGGAAAAATACGAG	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.429437	14525	15051	663-712	1.000000	80.8
atpF-atpH_p3	atpF-atpH	3	CGACTGGCCAGTTAACCAAA	CCTGAAGCGGAGGGAAAAAT	20	20	50.0	50.0	58.4	58.2	38.0	0.0	0.560553	14525	15051	648-697	1.000000	79.8
atpF-atpH_p4	atpF-atpH	4	CGACTGGCCAGTTAACCAAA	AGCGGAGGGAAAAATACGAG	20	20	50.0	50.0	58.4	57.7	38.0	0.0	0.711101	14525	15051	643-692	1.000000	78.5
atpF-atpH_p5	atpF-atpH	5	ATTAAACCCGAAACTCCCGG	CGGAGGGAAAAATACGAGGT	20	20	50.0	50.0	57.9	57.3	0.0	0.0	0.804163	14525	15051	661-710	1.000000	77.8
atpH-atpI_p1	atpH-atpI	1	AGCGGCAGAAATCATTGGAT	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	57.9	57.9	1.4	0.0	0.195711	15298	16459	1239-1241	1.000000	78.7
atpH-atpI_p2	atpH-atpI	2	AAGCAAGTCCTACAGCCAAC	TTTTGCAACTTTAGCTGCGG	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.235180	15298	16459	1276-1278	1.000000	78.4
atpH-atpI_p3	atpH-atpI	3	AACAGAAGCGGCAGAAATCA	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	58.1	57.9	1.4	0.0	0.236520	15298	16459	1245-1247	1.000000	78.4
atpH-atpI_p4	atpH-atpI	4	ACAGAAGCGGCAGAAATCAT	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	57.9	57.9	1.4	0.0	0.252541	15298	16459	1244-1246	1.000000	78.2
atpH-atpI_p5	atpH-atpI	5	AAGCGGCAGAAATCATTGGA	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	58.2	57.9	1.4	0.0	0.294628	15298	16459	1240-1242	1.000000	77.9
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	ATTCCCGCATTTCCATCTCC	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.0	58.4	0.0	14.6	0.368609	28768	30063	1361-1366	1.000000	78.4
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	ATTCCCGCATTTCCATCTCC	GCGACACCCAGATTTGAACT	20	20	50.0	50.0	58.0	58.5	0.0	0.0	0.489360	28768	30063	1386-1391	1.000000	77.4
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	GCATTTCCATCTCCGAGCAT	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.4	58.4	0.0	14.6	0.753180	28768	30063	1355-1360	1.000000	75.3
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	ATTCCCGCATTTCCATCTCC	TTTGTTGATCAGGCGACACC	20	20	50.0	50.0	58.0	58.8	0.0	0.0	0.778297	28768	30063	1398-1403	1.000000	75.1
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	ACATTCCCGCATTTCCATCT	AAAAGGATTTGCAGTCCCCC	20	20	45.0	50.0	57.6	58.4	0.0	14.6	0.797727	28768	30063	1363-1368	1.000000	75.0
petN-psbM_p1	petN-psbM	1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTCTTCATTCT	20	21	45.0	42.9	58.0	57.0	0.0	0.0	1.975331	31001	32041	1194-1196	1.000000	64.7
petN-psbM_p2	petN-psbM	2	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTCTTCATTCT	20	21	50.0	42.9	57.8	57.0	0.0	0.0	2.160415	31001	32041	1200-1202	1.000000	63.2
petN-psbM_p3	petN-psbM	3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTCTTCATTCT	20	21	50.0	42.9	57.6	57.0	0.0	0.0	2.320150	31001	32041	1140-1142	1.000000	62.0
petN-psbM_p4	petN-psbM	4	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTCTTCATTC	20	20	45.0	45.0	58.0	55.5	0.0	0.0	2.521758	31001	32041	1194-1196	1.000000	60.4
petN-psbM_p5	petN-psbM	5	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTCTTCATTC	20	20	50.0	45.0	57.8	55.5	0.0	0.0	2.706841	31001	32041	1200-1202	1.000000	58.9
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	50173	51067	942-976	1.000000	79.6
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	50173	51067	950-984	1.000000	79.1
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	50173	51067	974-1008	1.000000	78.8
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	50173	51067	982-1016	1.000000	78.2
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	50173	51067	956-990	1.000000	77.8
accD-psaI_p1	accD-psaI	1	AGCTACACGACTTTTTGCCT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	57.7	58.0	0.0	26.9	0.283121	62877	63532	773-779	1.000000	78.9
accD-psaI_p2	accD-psaI	2	CAGCTACACGACTTTTTGCC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.7	58.0	0.0	26.9	0.343122	62877	63532	774-780	1.000000	78.4
accD-psaI_p3	accD-psaI	3	AGCTACACGACTTTTTGCCT	AAGAAGCCATTGCAATTGCC	20	20	45.0	45.0	57.7	57.9	0.0	26.8	0.359654	62877	63532	779-785	1.000000	78.2
accD-psaI_p4	accD-psaI	4	CAGCTACACGACTTTTTGCC	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	57.7	57.9	0.0	26.8	0.419656	62877	63532	780-786	1.000000	77.8
accD-psaI_p5	accD-psaI	5	GCTACACGACTTTTTGCCTT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	56.9	58.0	0.0	26.9	1.086153	62877	63532	772-778	1.000000	72.4
rpl16_p1	rpl16	1	ACCCAAAACTTCTTTCCTTTGG	AGCAATTCCACAATTCTATAGGGT	22	24	40.9	37.5	57.2	58.0	5.6	0.0	6.771949	85197	86654	1548-1558	1.000000	40.7
rpl16_p2	rpl16	2	CCCAAAACTTCTTTCCTTTGGT	AGCAATTCCACAATTCTATAGGGT	22	24	40.9	37.5	57.2	58.0	10.6	0.0	6.771949	85197	86654	1547-1557	1.000000	40.7
rpl16_p3	rpl16	3	ACCCAAAACTTCTTTCCTTTGG	ACAAAAATGAGCAATTCCACA	22	21	40.9	33.3	57.2	54.8	5.6	0.0	6.930634	85197	86654	1557-1567	1.000000	40.7
rpl16_p4	rpl16	4	CCCAAAACTTCTTTCCTTTGGT	ACAAAAATGAGCAATTCCACA	22	21	40.9	33.3	57.2	54.8	10.6	0.0	6.930634	85197	86654	1556-1566	1.000000	40.7
rpl16_p5	rpl16	5	CCCAAAACTTCTTTCCTTTGGTT	AGCAATTCCACAATTCTATAGGGT	23	24	39.1	37.5	57.9	58.0	10.6	0.0	7.116175	85197	86654	1547-1557	1.000000	40.7
ycf2-trnL-CAA_p1	ycf2-trnL-CAA	1	TTCTATAGGAACCGGACGCT	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	57.9	58.0	9.5	15.9	0.070801	95613	97880	2479-2564	1.000000	82.2
ycf2-trnL-CAA_p2	ycf2-trnL-CAA	2	TTCTATAGGAACCGGACGCT	TGAAATGGTAGACACGCGAG	20	20	50.0	50.0	57.9	58.0	9.5	15.9	0.072171	95613	97880	2481-2566	1.000000	82.2
ycf2-trnL-CAA_p3	ycf2-trnL-CAA	3	TTCTATAGGAACCGGACGCT	ATGTCCGATTGGGGCCTATA	20	20	50.0	50.0	57.9	58.0	9.5	0.8	0.101833	95613	97880	2619-2704	1.000000	81.8
ycf2-trnL-CAA_p4	ycf2-trnL-CAA	4	TTCTATAGGAACCGGACGCT	TGTCCGATTGGGGCCTATAT	20	20	50.0	50.0	57.9	58.0	9.5	0.8	0.101833	95613	97880	2618-2703	1.000000	81.8
ycf2-trnL-CAA_p5	ycf2-trnL-CAA	5	TTCTATAGGAACCGGACGCT	CCAACACTCCACCTTTGTCA	20	20	50.0	50.0	57.9	57.9	9.5	0.0	0.114305	95613	97880	2573-2658	1.000000	81.7
ndhF-rpl32_p1	ndhF-rpl32	1	GTGGGACGAAAGGTATGACC	AAAAAGCTTTCAACGCTGCC	20	20	55.0	45.0	58.0	58.4	0.0	13.1	0.449135	116801	117844	1183-1188	1.000000	77.8
ndhF-rpl32_p2	ndhF-rpl32	2	TGTTGCCGTCGGAAAAAGTA	AAAAAGCTTTCAACGCTGCC	20	20	45.0	45.0	58.0	58.4	17.5	13.1	0.478359	116801	117844	1236-1241	1.000000	77.5
ndhF-rpl32_p3	ndhF-rpl32	3	ACGAAAGGTATGACCCAGGA	AAAAAGCTTTCAACGCTGCC	20	20	50.0	45.0	58.1	58.4	0.0	13.1	0.482203	116801	117844	1178-1183	1.000000	77.5
ndhF-rpl32_p4	ndhF-rpl32	4	GTGGGACGAAAGGTATGACC	CGCTGCCCAATATCCCTTTT	20	20	55.0	50.0	58.0	58.6	0.0	0.0	0.617161	116801	117844	1170-1175	1.000000	76.4
ndhF-rpl32_p5	ndhF-rpl32	5	TTTGTTGCCGTCGGAAAAAG	AAAAAGCTTTCAACGCTGCC	20	20	45.0	45.0	57.8	58.4	17.5	13.1	0.625482	116801	117844	1238-1243	1.000000	76.3
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	CCCTTTCTACCGGGAATTCG	AGCGTGTCTACCGATTTCAC	20	20	55.0	50.0	58.1	58.0	6.3	0.0	0.058365	118019	119057	1126-1133	1.000000	80.4
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	CGAAATCCCTTTCTACCGGG	AGCGTGTCTACCGATTTCAC	20	20	55.0	50.0	58.1	58.0	6.3	0.0	0.058365	118019	119057	1132-1139	1.000000	80.4
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	CCCTTTCTACCGGGAATTCG	CAGCGTGTCTACCGATTTCA	20	20	55.0	50.0	58.1	58.0	6.3	0.0	0.059735	118019	119057	1127-1134	1.000000	80.4
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	CGAAATCCCTTTCTACCGGG	CAGCGTGTCTACCGATTTCA	20	20	55.0	50.0	58.1	58.0	6.3	0.0	0.059735	118019	119057	1133-1140	1.000000	80.4
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	ACCGGGAATTCGAAAAGCTT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.1	58.0	6.3	0.0	0.102193	118019	119057	1118-1125	1.000000	80.0
ycf1_p1	ycf1	1	TGCTTGTGACTGTGAGAAACA	CCCTTAATATTCCAAAAATCAATGCA	21	26	42.9	30.8	58.0	57.0	0.0	0.0	7.976732	130717	130866	227-227	1.000000	41.4
ycf1_p2	ycf1	2	TGCTTGTGACTGTGAGAAACA	ACCCTTAATATTCCAAAAATCAATGC	21	26	42.9	30.8	58.0	57.0	0.0	0.0	7.978322	130717	130866	228-228	1.000000	41.4
ycf1_p3	ycf1	3	TGCTTGTGACTGTGAGAAACA	CCCTTAATATTCCAAAAATCAATGCAA	21	27	42.9	29.6	58.0	57.6	0.0	0.0	8.408950	130717	130866	227-227	1.000000	41.4
ycf1_p4	ycf1	4	TGCTTGTGACTGTGAGAAACA	ACCCTTAATATTCCAAAAATCAATGCA	21	27	42.9	29.6	58.0	58.5	0.0	0.0	8.468266	130717	130866	228-228	1.000000	41.4
ycf1_p5	ycf1	5	TGCTTGTGACTGTGAGAAACA	CCCTTAATATTCCAAAAATCAATGC	21	25	42.9	32.0	58.0	55.5	0.0	0.0	8.472443	130717	130866	227-227	1.000000	41.4
trnL-CAA-ycf2_p1	trnL-CAA-ycf2	1	AAATGGTAGACACGCGAGAC	TTCTATAGGAACCGGACGCT	20	20	50.0	50.0	58.0	57.9	15.9	9.5	0.070801	149571	151838	2479-2564	1.000000	82.2
trnL-CAA-ycf2_p2	trnL-CAA-ycf2	2	TGAAATGGTAGACACGCGAG	TTCTATAGGAACCGGACGCT	20	20	50.0	50.0	58.0	57.9	15.9	9.5	0.072171	149571	151838	2481-2566	1.000000	82.2
trnL-CAA-ycf2_p3	trnL-CAA-ycf2	3	ATGTCCGATTGGGGCCTATA	TTCTATAGGAACCGGACGCT	20	20	50.0	50.0	58.0	57.9	0.8	9.5	0.101833	149571	151838	2619-2704	1.000000	81.8
trnL-CAA-ycf2_p4	trnL-CAA-ycf2	4	TGTCCGATTGGGGCCTATAT	TTCTATAGGAACCGGACGCT	20	20	50.0	50.0	58.0	57.9	0.8	9.5	0.101833	149571	151838	2618-2703	1.000000	81.8
trnL-CAA-ycf2_p5	trnL-CAA-ycf2	5	CCAACACTCCACCTTTGTCA	TTCTATAGGAACCGGACGCT	20	20	50.0	50.0	57.9	57.9	0.0	9.5	0.114305	149571	151838	2573-2658	1.000000	81.7