primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	atpF-atpH	1	CAGTGACCCAAGGAAACGAA	TTTGCAAATCCCTTTGTTTAA	20	21	50.0	28.6	58.0	52.6	0.0	8.1	6.394089	11773	12250	533-568	1.000000	49.7
atpF-atpH_p2	atpF-atpH	2	AGTGACCCAAGGAAACGAAA	ATTTGCAAATCCCTTTGTTT	20	20	45.0	30.0	57.3	52.3	0.0	19.0	6.421555	11773	12250	533-568	1.000000	49.7
atpF-atpH_p3	atpF-atpH	3	CAGTGACCCAAGGAAACGAA	ATTTGCAAATCCCTTTGTTT	20	20	50.0	30.0	58.0	52.3	0.0	19.0	5.738336	11773	12250	534-569	1.000000	49.6
atpF-atpH_p4	atpF-atpH	4	CAGTGACCCAAGGAAACGAA	ATTTGCAAATCCCTTTGTTTA	20	21	50.0	28.6	58.0	52.4	0.0	19.0	6.631252	11773	12250	534-569	1.000000	49.6
atpF-atpH_p5	atpF-atpH	5	CAGTGACCCAAGGAAACGAA	CTTTTATTTGCAAATCCCTTTGT	20	23	50.0	30.4	58.0	54.6	0.0	27.3	6.451032	11773	12250	539-574	1.000000	49.5
petN-psbM_p1	petN-psbM	1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTGTTCATTCT	20	21	45.0	42.9	58.0	57.6	0.0	0.0	1.413468	28052	29232	1285-1336	1.000000	76.6
petN-psbM_p2	petN-psbM	2	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.8	57.6	0.0	0.0	1.598551	28052	29232	1291-1342	1.000000	75.1
petN-psbM_p3	petN-psbM	3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.6	57.6	0.0	0.0	1.758286	28052	29232	1231-1282	1.000000	74.0
petN-psbM_p4	petN-psbM	4	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTGTTCATTC	20	20	45.0	45.0	58.0	56.1	0.0	0.0	1.936016	28052	29232	1285-1336	1.000000	72.4
petN-psbM_p5	petN-psbM	5	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTGTTCATTC	20	20	50.0	45.0	57.8	56.1	0.0	0.0	2.121100	28052	29232	1291-1342	1.000000	71.0
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	ATACTAGGTCGGGACTGACG	TGGCAATATGTCTACGCTGG	20	20	55.0	50.0	58.0	57.8	5.3	0.0	0.274389	30492	30881	502-513	1.000000	85.3
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	ATACTAGGTCGGGACTGACG	GGCAATATGTCTACGCTGGT	20	20	55.0	50.0	58.0	57.8	5.3	0.0	0.275941	30492	30881	501-512	1.000000	85.3
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	ATACTAGGTCGGGACTGACG	GGGGACGGACTGTAAATTCG	20	20	55.0	55.0	58.0	58.4	5.3	0.0	0.386943	30492	30881	523-534	1.000000	84.1
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	ATACTAGGTCGGGACTGACG	GGGACGGACTGTAAATTCGT	20	20	55.0	50.0	58.0	57.6	5.3	0.0	0.410621	30492	30881	522-533	1.000000	84.0
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	CATCGGATACTAGGTCGGGA	TGGCAATATGTCTACGCTGG	20	20	55.0	50.0	57.8	57.8	0.0	0.0	0.434683	30492	30881	508-519	1.000000	84.0
trnY-GUA-trnE-UUC_p1	trnY-GUA-trnE-UUC	1	CCAGCGTAGACATATTGCCA	CTCTCTTTCAAGGAGGCAGC	20	20	50.0	55.0	57.8	58.3	0.0	0.0	0.502330	30966	31033	177-200	1.000000	81.8
trnY-GUA-trnE-UUC_p2	trnY-GUA-trnE-UUC	2	ACCAGCGTAGACATATTGCC	CTCTCTTTCAAGGAGGCAGC	20	20	50.0	55.0	57.8	58.3	0.0	0.0	0.503882	30966	31033	178-201	1.000000	81.7
trnY-GUA-trnE-UUC_p3	trnY-GUA-trnE-UUC	3	CGAATTTACAGTCCGTCCCC	CTCTCTTTCAAGGAGGCAGC	20	20	55.0	55.0	58.4	58.3	0.0	0.0	0.614884	30966	31033	156-179	1.000000	81.1
trnY-GUA-trnE-UUC_p4	trnY-GUA-trnE-UUC	4	ACGAATTTACAGTCCGTCCC	CTCTCTTTCAAGGAGGCAGC	20	20	50.0	55.0	57.6	58.3	0.0	0.0	0.638561	30966	31033	157-180	1.000000	80.9
trnY-GUA-trnE-UUC_p5	trnY-GUA-trnE-UUC	5	GCCAACGAATTTACAGTCCG	CTCTCTTTCAAGGAGGCAGC	20	20	50.0	55.0	57.5	58.3	0.0	0.0	0.784075	30966	31033	161-184	1.000000	79.6
trnT-UGU-trnF-GAA_p1	trnT-UGU-trnF-GAA	1	GCGATGCTCTAACCTCTGAG	TTTGAACTGGTGACACGAGG	20	20	55.0	50.0	57.9	58.1	0.0	20.1	0.184636	46964	48765	1813-1950	1.000000	86.8
trnT-UGU-trnF-GAA_p2	trnT-UGU-trnF-GAA	2	GCGATGCTCTAACCTCTGAG	TTAACCACATGCCAGGAACC	20	20	55.0	50.0	57.9	58.1	0.0	6.5	0.214868	46964	48765	1836-1973	1.000000	86.6
trnT-UGU-trnF-GAA_p3	trnT-UGU-trnF-GAA	3	GCGATGCTCTAACCTCTGAG	CACATGCCAGGAACCAGATT	20	20	55.0	50.0	57.9	58.2	0.0	0.0	0.289372	46964	48765	1831-1968	1.000000	86.0
trnT-UGU-trnF-GAA_p4	trnT-UGU-trnF-GAA	4	GGAATCGAACCGATGACCAT	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.8	58.1	15.5	20.1	0.292389	46964	48765	1845-1982	1.000000	85.9
trnT-UGU-trnF-GAA_p5	trnT-UGU-trnF-GAA	5	GGAATCGAACCGATGACCAT	TTAACCACATGCCAGGAACC	20	20	50.0	50.0	57.8	58.1	15.5	6.5	0.322621	46964	48765	1868-2005	1.000000	85.6
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CCTTCGTTAATTGGGGCCAA	AGTGCATGTTGGGTCTTTGA	20	20	50.0	45.0	58.4	57.6	4.4	0.0	0.811855	51303	52389	1282-1318	1.000000	79.0
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TAATTGGGGCCAAAACTCCG	AGTGCATGTTGGGTCTTTGA	20	20	50.0	45.0	58.4	57.6	4.4	0.0	0.811855	51303	52389	1275-1311	1.000000	79.0
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TGGTCCTTCGTTAATTGGGG	AGTGCATGTTGGGTCTTTGA	20	20	50.0	45.0	57.5	57.6	0.0	0.0	0.863699	51303	52389	1286-1322	1.000000	78.6
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CCTTCGTTAATTGGGGCCAA	AGAAGGTCTACGGTTCGAGA	20	20	50.0	50.0	58.4	57.5	4.4	0.0	0.935020	51303	52389	1215-1251	1.000000	78.3
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TAATTGGGGCCAAAACTCCG	AGAAGGTCTACGGTTCGAGA	20	20	50.0	50.0	58.4	57.5	4.4	0.0	0.935020	51303	52389	1208-1244	1.000000	78.3
rpl33-rps18_p1	rpl33-rps18	1	TACGCCTAGTCGATTGGAGT	CAAACGCCTACGAAAAGCTC	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.276198	68761	68971	323-353	1.000000	86.7
rpl33-rps18_p2	rpl33-rps18	2	TACGCCTAGTCGATTGGAGT	AATCAATTCGATCCCCCGAC	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.304561	68761	68971	354-384	1.000000	86.1
rpl33-rps18_p3	rpl33-rps18	3	CGCCTAGTCGATTGGAGTTG	CAAACGCCTACGAAAAGCTC	20	20	55.0	50.0	58.4	57.8	5.6	0.0	0.666830	68761	68971	321-351	1.000000	83.7
rpl33-rps18_p4	rpl33-rps18	4	CGCCTAGTCGATTGGAGTTG	AATCAATTCGATCCCCCGAC	20	20	55.0	50.0	58.4	57.7	5.6	0.0	0.695194	68761	68971	352-382	1.000000	83.0
rpl33-rps18_p5	rpl33-rps18	5	CGACACAATACGCCTAGTCG	CAAACGCCTACGAAAAGCTC	20	20	55.0	50.0	58.5	57.8	0.0	0.0	0.763948	68761	68971	331-361	1.000000	82.7
rps12-clpP_p1	rps12-clpP	1	AGAGCGGGGGATTTTGTTAC	TGTCACCAGAACAAGCCAAA	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.260576	70826	70990	305-313	0.944444	82.6
rps12-clpP_p2	rps12-clpP	2	GAGCGGGGGATTTTGTTACA	TGTCACCAGAACAAGCCAAA	20	20	50.0	45.0	58.2	57.9	0.0	0.0	0.307319	70826	70990	304-312	0.944444	82.3
rps12-clpP_p3	rps12-clpP	3	AGAGCGGGGGATTTTGTTAC	ATGTCACCAGAACAAGCCAA	20	20	50.0	45.0	57.9	57.6	0.0	0.0	0.487584	70826	70990	306-314	0.944444	80.8
rps12-clpP_p4	rps12-clpP	4	GAGCGGGGGATTTTGTTACA	ATGTCACCAGAACAAGCCAA	20	20	50.0	45.0	58.2	57.6	0.0	0.0	0.534327	70826	70990	305-313	0.944444	80.4
rps12-clpP_p5	rps12-clpP	5	GAAGAGCGGGGGATTTTGTT	TGTCACCAGAACAAGCCAAA	20	20	50.0	45.0	58.5	57.9	0.0	0.0	0.590457	70826	70990	307-315	0.944444	79.9
rpl16_p1	rpl16	1	CGCACCAAAAACTCGAGTTC	GTTAATAGAGGGCAGTCCGC	20	20	50.0	55.0	58.0	58.1	29.8	0.0	0.151551	82523	83991	2439-2567	0.777778	73.6
rpl16_p2	rpl16	2	CGCACCAAAAACTCGAGTTC	AAATTGCAGGGCGTATCGAT	20	20	50.0	45.0	58.0	58.0	29.8	2.2	0.051399	82523	83991	2200-2225	0.611111	64.8
rpl16_p3	rpl16	3	CCCTTTCCTTTCGTTCCACA	AAATTGCAGGGCGTATCGAT	20	20	50.0	45.0	58.0	58.0	0.0	2.2	0.057022	82523	83991	1801-1823	0.611111	64.7
rpl16_p4	rpl16	4	ACCATTGTCACGCTTGAGTT	AAATTGCAGGGCGTATCGAT	20	20	45.0	45.0	58.0	58.0	0.0	2.2	0.065363	82523	83991	2119-2144	0.611111	64.7
rpl16_p5	rpl16	5	ATCGCACCAAAAACTCGAGT	AAATTGCAGGGCGTATCGAT	20	20	45.0	45.0	58.1	58.0	13.6	2.2	0.153728	82523	83991	2202-2227	0.611111	64.0
rpl22-rps19_p1	rpl22-rps19	1	ACTTCTACATCCGGGTCACT	GGACACGCGAGAAACGATAA	20	20	50.0	50.0	57.8	58.4	0.0	15.9	0.612198	85320	85413	128-167	1.000000	85.1
rpl22-rps19_p2	rpl22-rps19	2	ACTTCTACATCCGGGTCACT	GCACCTACTCGGAATTTCCA	20	20	50.0	50.0	57.8	57.6	0.0	15.6	0.629895	85320	85413	149-188	1.000000	84.2
rpl22-rps19_p3	rpl22-rps19	3	ACTTCTACATCCGGGTCACT	TGCACCTACTCGGAATTTCC	20	20	50.0	50.0	57.8	57.6	0.0	5.3	0.629895	85320	85413	150-189	1.000000	84.2
rpl22-rps19_p4	rpl22-rps19	4	ACTTCTACATCCGGGTCACT	CTCGGAATTTCCAGGGACAC	20	20	50.0	55.0	57.8	58.3	0.0	15.6	0.494238	85320	85413	142-181	0.944444	82.8
rpl22-rps19_p5	rpl22-rps19	5	TCTACATCCGGGTCACTCTT	CTCGGAATTTCCAGGGACAC	20	20	50.0	55.0	57.5	58.3	0.0	15.6	0.787821	85320	85413	139-178	0.944444	80.5
ndhF-trnL-UAG_p1	ndhF-trnL-UAG	1	CAAAGGGTATGATCCACGCA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.0	58.0	12.3	0.0	0.044788	113168	114038	624-970	1.000000	91.6
ndhF-trnL-UAG_p2	ndhF-trnL-UAG	2	CAAAGGGTATGATCCACGCA	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.0	58.0	12.3	0.0	0.046158	113168	114038	625-971	1.000000	91.6
ndhF-trnL-UAG_p3	ndhF-trnL-UAG	3	CAAAGGGTATGATCCACGCA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.0	57.9	12.3	0.0	0.161235	113168	114038	634-980	1.000000	90.7
ndhF-trnL-UAG_p4	ndhF-trnL-UAG	4	CAAAGGGTATGATCCACGCA	CCTAAGAGCAGCGTGTCTAC	20	20	50.0	55.0	58.0	57.8	12.3	0.0	0.233252	113168	114038	633-979	1.000000	90.1
ndhF-trnL-UAG_p5	ndhF-trnL-UAG	5	CAAAGGGTATGATCCACGCA	CTAAGAGCAGCGTGTCTACC	20	20	50.0	55.0	58.0	57.8	12.3	0.0	0.233252	113168	114038	632-978	1.000000	90.1
ndhG-ndhI_p1	ndhG-ndhI	1	ATTGGCCCAGGTAAATCCAT	GGTAACCCGATCGATTCACG	20	20	45.0	55.0	56.8	58.5	0.0	18.7	1.690195	118709	119089	426-449	1.000000	77.6
ndhG-ndhI_p2	ndhG-ndhI	2	GTATTGGCCCAGGTAAATCCA	GGTAACCCGATCGATTCACG	21	20	47.6	55.0	57.7	58.5	0.0	18.7	1.822116	118709	119089	428-451	1.000000	76.5
ndhG-ndhI_p3	ndhG-ndhI	3	TGTATTGGCCCAGGTAAATCC	GGTAACCCGATCGATTCACG	21	20	47.6	55.0	57.7	58.5	0.0	18.7	1.822116	118709	119089	429-452	1.000000	76.5
ndhG-ndhI_p4	ndhG-ndhI	4	AATCATGTATTGGCCCAGGT	GGTAACCCGATCGATTCACG	20	20	45.0	55.0	56.8	58.5	0.0	18.7	1.667824	118709	119089	437-457	0.944444	75.0
ndhG-ndhI_p5	ndhG-ndhI	5	AAATCATGTATTGGCCCAGGT	GGTAACCCGATCGATTCACG	21	20	42.9	55.0	57.6	58.5	0.0	18.7	1.918872	118709	119089	438-458	0.944444	73.0
ycf1_p1	ycf1	1	TACCTCCTTCCCCAACCAAT	CGGGACAATCTGAGCAATGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.097251	127022	127141	849-870	0.833333	82.5
ycf1_p2	ycf1	2	CTGGTAATTCAGCACCGTCA	CGGGACAATCTGAGCAATGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.121726	127022	127141	1156-1165	0.777778	77.2
ycf1_p3	ycf1	3	GTTGAAGCACTGACCCAGAA	CGGGACAATCTGAGCAATGA	20	20	50.0	50.0	58.0	58.0	0.5	0.0	0.061101	127022	127141	745-751	0.777778	76.6
ycf1_p4	ycf1	4	GCACCGTCATAGTTGCAGTA	CGGGACAATCTGAGCAATGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.033002	127022	127141	1145-1154	0.666667	72.2
ycf1_p5	ycf1	5	GGAATTCGTTTTCGGGCAAG	CGGGACAATCTGAGCAATGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.051232	127022	127141	1340-1340	0.611111	64.4