primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	81	482	433-535	1.000000	90.4
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.208010	81	482	447-549	1.000000	90.3
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	81	482	446-548	1.000000	90.3
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	81	482	448-550	1.000000	90.3
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.465225	81	482	482-584	1.000000	88.1
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	AGGAACTTCGTCCTAATCAATCC	20	23	50.0	43.5	58.2	58.0	20.3	0.0	3.193205	4354	5069	728-776	1.000000	62.9
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAAGCCGAGTACTCTACCGT	GGAACTTCGTCCTAATCAATCCT	20	23	50.0	43.5	58.2	58.0	20.3	0.0	3.193205	4354	5069	727-775	1.000000	62.9
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAGCCGAGTACTCTACCGTT	AGGAACTTCGTCCTAATCAATCC	20	23	50.0	43.5	58.2	58.0	20.3	0.0	3.193205	4354	5069	727-775	1.000000	62.9
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	GGAACTTCGTCCTAATCAATCCT	20	23	50.0	43.5	58.2	58.0	20.3	0.0	3.193205	4354	5069	726-774	1.000000	62.9
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CCGCACTTAAAAGCCGAGTA	GGAACTTCGTCCTAATCAATCCT	20	23	50.0	43.5	58.3	58.0	5.3	0.0	3.307591	4354	5069	736-784	1.000000	61.9
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6204	7410	1210-1308	1.000000	90.3
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	CACAACGGATCCTGTCCTTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.3	58.1	10.8	16.9	0.349248	6204	7410	1230-1321	0.933333	86.5
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CGGATCCTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	57.7	58.1	10.8	16.9	0.377678	6204	7410	1225-1316	0.933333	86.3
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	GGATCCTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	57.7	58.1	3.8	16.9	0.377678	6204	7410	1224-1315	0.933333	86.3
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	GATCCTGTCCTTCAAGTCGC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	0.0	16.9	0.429912	6204	7410	1223-1314	0.933333	85.9
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	20	21	45.0	38.1	58.0	55.5	0.0	0.0	3.574049	8712	9501	849-879	1.000000	57.9
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	ATTAGCAATCCGCCGCTTTA	AGAACGAATCACACTTTTACCAC	20	23	45.0	39.1	58.0	57.3	0.0	0.0	3.706765	8712	9501	852-882	1.000000	56.8
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	20	23	45.0	39.1	58.0	57.3	0.0	0.0	3.706765	8712	9501	851-881	1.000000	56.8
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.2	55.5	0.0	0.0	3.687191	8712	9501	898-928	1.000000	56.7
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	ATTAGCAATCCGCCGCTTTA	AACGAATCACACTTTTACCACT	20	22	45.0	36.4	58.0	56.2	0.0	0.0	3.814520	8712	9501	850-880	1.000000	56.0
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	TACCTGTCATGCTCCTTGGA	20	20	45.0	50.0	58.0	58.0	1.4	0.0	0.087178	14112	15276	1292-1311	1.000000	83.8
atpH-atpI_p2	atpH-atpI	2	AATAACGGAAGCGGCAGAAA	TACCTGTCATGCTCCTTGGA	20	20	45.0	50.0	58.2	58.0	1.4	0.0	0.232716	14112	15276	1293-1312	1.000000	82.6
atpH-atpI_p3	atpH-atpI	3	GCCAATCCAGCAGCAATAAC	TACCTGTCATGCTCCTTGGA	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.263178	14112	15276	1307-1326	1.000000	82.3
atpH-atpI_p4	atpH-atpI	4	TCCAGCAGCAATAACGGAAG	TACCTGTCATGCTCCTTGGA	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.310952	14112	15276	1302-1321	1.000000	82.0
atpH-atpI_p5	atpH-atpI	5	ATCCAGCAGCAATAACGGAA	TACCTGTCATGCTCCTTGGA	20	20	45.0	50.0	57.6	58.0	0.0	0.0	0.467159	14112	15276	1303-1322	1.000000	80.7
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGTAGAAAAGCGGTAGGAACT	CCCGTATTTCCCTGGGATTG	21	20	42.9	55.0	56.4	58.0	0.0	17.9	2.629866	30907	31980	1215-1247	1.000000	64.2
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AGTAGAAAAGCGGTAGGAACT	TCCCGTATTTCCCTGGGATT	21	20	42.9	50.0	56.4	58.1	0.0	17.9	2.709475	30907	31980	1216-1248	1.000000	63.6
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AAGTAGAAAAGCGGTAGGAACT	CCCGTATTTCCCTGGGATTG	22	20	40.9	55.0	57.1	58.0	0.0	17.9	2.905239	30907	31980	1216-1248	1.000000	62.0
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AGAAAAGCGGTAGGAACTAGA	CCCGTATTTCCCTGGGATTG	21	20	42.9	55.0	56.1	58.0	0.0	17.9	2.908951	30907	31980	1212-1244	1.000000	62.0
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AAGTAGAAAAGCGGTAGGAACT	TCCCGTATTTCCCTGGGATT	22	20	40.9	50.0	57.1	58.1	0.0	17.9	2.984848	30907	31980	1217-1249	1.000000	61.4
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	32379	33238	973-1010	1.000000	85.8
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	57.8	58.1	0.0	0.0	0.223247	32379	33238	952-989	1.000000	85.4
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.245401	32379	33238	949-986	1.000000	85.3
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	32379	33238	935-972	1.000000	85.2
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	20	20	55.0	55.0	57.8	58.2	0.0	0.0	0.310083	32379	33238	970-1007	1.000000	84.6
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	20	20	55.0	50.0	58.1	58.1	7.6	0.0	0.177181	33311	34529	1355-1379	1.000000	86.1
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.199336	33311	34529	1358-1382	1.000000	86.0
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	33311	34529	1345-1369	1.000000	85.1
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.332292	33311	34529	1348-1372	1.000000	84.9
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GGCGTAAGTCATCGGTTCAA	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.395920	33311	34529	1332-1356	1.000000	84.5
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TTGCTAATCGGGGCTAACAC	TGAATCAGAGCACATGGAGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.066785	52657	53757	1355-1417	1.000000	88.1
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TTGCTAATCGGGGCTAACAC	ATCTAGGCCAGAGACAGACC	20	20	50.0	55.0	58.0	57.9	0.0	5.0	0.113334	52657	53757	1426-1490	1.000000	87.5
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	AGGGGGCCATTTTCATTGTT	TGAATCAGAGCACATGGAGC	20	20	45.0	50.0	58.0	58.0	4.4	0.0	0.077412	52657	53757	1929-1991	1.000000	87.0
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TGGTTCAGGCATTTGCTCAT	TGAATCAGAGCACATGGAGC	20	20	45.0	50.0	58.1	58.0	2.1	0.0	0.106617	52657	53757	1964-2026	1.000000	86.8
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	AGGGGGCCATTTTCATTGTT	ATCTAGGCCAGAGACAGACC	20	20	45.0	55.0	58.0	57.9	4.4	5.0	0.123961	52657	53757	2000-2064	1.000000	86.5
accD-psaI_p1	accD-psaI	1	TCCAGAAGGGTTTATTCGACC	AAGAAGCCATTGCAATTGCC	21	20	47.6	45.0	57.4	57.9	0.0	26.8	1.725703	61204	61960	932-952	1.000000	71.6
accD-psaI_p2	accD-psaI	2	CCAGAAGGGTTTATTCGACCT	AAGAAGCCATTGCAATTGCC	21	20	47.6	45.0	57.4	57.9	0.0	26.8	1.732679	61204	61960	931-951	1.000000	71.6
accD-psaI_p3	accD-psaI	3	TCGACCTAATTGTACCACGT	AAGAAGCCATTGCAATTGCC	20	20	45.0	45.0	56.3	57.9	0.0	26.8	1.795426	61204	61960	917-937	1.000000	71.1
accD-psaI_p4	accD-psaI	4	TCCAGAAGGGTTTATTCGACC	TGCAATTGCCGGAAAGACTA	21	20	47.6	45.0	57.4	57.8	0.0	26.9	1.814829	61204	61960	922-942	1.000000	71.0
accD-psaI_p5	accD-psaI	5	AGTTATTTAAGCTCCACGCCT	AAGAAGCCATTGCAATTGCC	21	20	42.9	45.0	57.4	57.9	0.0	26.8	1.732679	61204	61960	872-892	0.933333	68.2
rps19-rpl2_p1	rps19-rpl2	1	GCCCGGGACCAAGTTACTAT	TTGATTCTTCGTCGCCGTAG	20	20	55.0	50.0	59.2	58.1	29.3	0.0	1.260580	86532	86593	197-218	1.000000	77.0
rps19-rpl2_p2	rps19-rpl2	2	GCCCGGGACCAAGTTACTAT	ATTTGATTCTTCGTCGCCGT	20	20	55.0	45.0	59.2	58.3	29.3	0.0	1.442713	86532	86593	199-220	1.000000	75.9
rps19-rpl2_p3	rps19-rpl2	3	GCCCGGGACCAAGTTACTAT	CACAACCCCTTGGGGTTATC	20	20	55.0	55.0	59.2	58.2	29.3	35.0	1.317396	86532	86593	264-285	1.000000	75.4
rps19-rpl2_p4	rps19-rpl2	4	CCCGGGACCAAGTTACTATT	TTGATTCTTCGTCGCCGTAG	20	20	50.0	50.0	56.6	58.1	18.5	0.0	1.483856	86532	86593	196-217	1.000000	75.3
rps19-rpl2_p5	rps19-rpl2	5	GCCCGGGACCAAGTTACTAT	TTGGGGTTATCCTGCACTTG	20	20	55.0	50.0	59.2	57.8	29.3	0.0	1.379100	86532	86593	255-276	1.000000	75.1
ycf1-ndhF_p1	ycf1-ndhF	1	ACAGGAACAAGAGGGATCCA	GCGGACGCATCTCTTCTTAT	20	20	50.0	50.0	58.0	57.9	15.2	12.3	0.186328	111910	111939	300-305	1.000000	86.7
ycf1-ndhF_p2	ycf1-ndhF	2	ACAGGAACAAGAGGGATCCA	TCAACCAAGAGGGAGGGAAT	20	20	50.0	50.0	58.0	57.9	15.2	0.0	0.104549	111910	111939	747-752	0.933333	80.8
ycf1-ndhF_p3	ycf1-ndhF	3	AGGGTGGAAAGTGAGGAAGA	TCAACCAAGAGGGAGGGAAT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.178160	111910	111939	812-817	0.933333	79.9
ycf1-ndhF_p4	ycf1-ndhF	4	ACAGGAACAAGAGGGATCCA	TTCAACCAAGAGGGAGGGAA	20	20	50.0	50.0	58.0	58.2	15.2	0.0	0.217001	111910	111939	748-753	0.933333	79.9
ycf1-ndhF_p5	ycf1-ndhF	5	ACAGGAACAAGAGGGATCCA	CCAAGAGGGAGGGAATTTGG	20	20	50.0	55.0	58.0	57.9	15.2	0.0	0.194766	111910	111939	743-748	0.866667	76.4
ndhF-rpl32_p1	ndhF-rpl32	1	TGAAAGGTATGATCCACGCA	TGATGTCGAAGTGCGTTTTT	20	20	45.0	40.0	56.9	56.6	0.0	0.0	2.479438	114169	114955	836-860	1.000000	68.2
ndhF-rpl32_p2	ndhF-rpl32	2	TGAAAGGTATGATCCACGCA	TTGATGTCGAAGTGCGTTTT	20	20	45.0	40.0	56.9	56.6	0.0	0.0	2.479438	114169	114955	837-861	1.000000	68.2
ndhF-rpl32_p3	ndhF-rpl32	3	TGAAAGGTATGATCCACGCA	TTTGATGTCGAAGTGCGTTT	20	20	45.0	40.0	56.9	56.6	0.0	0.0	2.479438	114169	114955	838-862	1.000000	68.2
ndhF-rpl32_p4	ndhF-rpl32	4	TGAAAGGTATGATCCACGCA	TTTTGATGTCGAAGTGCGTT	20	20	45.0	40.0	56.9	56.6	0.0	0.0	2.479438	114169	114955	839-863	1.000000	68.2
ndhF-rpl32_p5	ndhF-rpl32	5	TGAAAGGTATGATCCACGCA	TTTTTGATGTCGAAGTGCGT	20	20	45.0	40.0	56.9	56.6	0.0	0.0	2.479438	114169	114955	840-864	1.000000	68.2
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TTTGTACGCCAAACAAAAAT	TCCTAAGAGCAGCGTGTCTA	20	20	30.0	50.0	52.8	57.9	4.0	0.0	5.373178	115124	116060	893-1006	1.000000	49.9
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TTTGTACGCCAAACAAAAAT	AGCAGCGTGTCTACCAATTT	20	20	30.0	45.0	52.8	57.8	4.0	0.0	5.433905	115124	116060	886-999	1.000000	49.9
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TTTGTACGCCAAACAAAAAT	CCTAAGAGCAGCGTGTCTAC	20	20	30.0	55.0	52.8	57.8	4.0	0.0	5.445196	115124	116060	892-1005	1.000000	49.9
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TTTGTACGCCAAACAAAAAT	CTAAGAGCAGCGTGTCTACC	20	20	30.0	55.0	52.8	57.8	4.0	0.0	5.445196	115124	116060	891-1004	1.000000	49.9
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TTTGTACGCCAAACAAAAAT	TCGAACCGAGATGCATTAGC	20	20	30.0	50.0	52.8	58.1	4.0	0.0	5.395959	115124	116060	919-1032	1.000000	49.7
rps15-ycf1_p1	rps15-ycf1	1	AGTCGTTGACGTTTTCCCAA	ATTGTCTCCTGCTTCGACTG	20	20	45.0	50.0	58.0	57.6	3.2	0.0	0.403811	125394	125789	1230-1245	1.000000	81.7
rps15-ycf1_p2	rps15-ycf1	2	ATGAGTCGAAGAACACCGTG	ATTGTCTCCTGCTTCGACTG	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.442800	125394	125789	1512-1527	1.000000	81.2
rps15-ycf1_p3	rps15-ycf1	3	AGTCGTTGACGTTTTCCCAA	TGTCTCCTGCTTCGACTGAT	20	20	45.0	50.0	58.0	58.4	3.2	0.0	0.476676	125394	125789	1228-1243	1.000000	81.2
rps15-ycf1_p4	rps15-ycf1	4	GCCTAATTGTTCGGGTCCAT	ATTGTCTCCTGCTTCGACTG	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.429590	125394	125789	1696-1711	1.000000	80.9
rps15-ycf1_p5	rps15-ycf1	5	GCTAGGAGTCGTTGACGTTT	ATTGTCTCCTGCTTCGACTG	20	20	50.0	50.0	57.9	57.6	3.2	0.0	0.438548	125394	125789	1236-1251	0.933333	78.2
ycf1_p1	ycf1	1	AGTGGTACGCCCTCTTGATA	TGAACAGGCCATAAGAACGG	20	20	50.0	50.0	57.8	57.9	0.0	5.0	0.262517	129776	129895	822-822	1.000000	84.4
ycf1_p2	ycf1	2	TGGTACGCCCTCTTGATAGT	TGAACAGGCCATAAGAACGG	20	20	50.0	50.0	57.8	57.9	0.0	5.0	0.262517	129776	129895	820-820	1.000000	84.4
ycf1_p3	ycf1	3	AACTGTTTTGCGCCTTTGAG	TGAACAGGCCATAAGAACGG	20	20	45.0	50.0	57.8	57.9	4.2	5.0	0.316276	129776	129895	1027-1057	1.000000	83.4
ycf1_p4	ycf1	4	AGTCACCCGATTGTTGTTCC	TGAACAGGCCATAAGAACGG	20	20	50.0	50.0	58.1	57.9	0.0	5.0	0.215740	129776	129895	803-803	0.933333	81.1
ycf1_p5	ycf1	5	CCCTCTTGATAGTCACCCGA	TGAACAGGCCATAAGAACGG	20	20	55.0	50.0	58.2	57.9	0.0	5.0	0.331288	129776	129895	813-813	0.933333	80.1