primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	matK-rps16	1	TCCTGAAGGAGTAGCGGATA	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	57.0	58.2	0.0	1.3	1.234783	3493	6130	2767-2796	1.000000	80.2
matK-rps16_p2	matK-rps16	2	TCCTGAAGGAGTAGCGGATAT	GGTGCTCAACCTACAGGAAC	21	20	47.6	55.0	57.5	58.2	0.0	1.3	1.717823	3493	6130	2767-2796	1.000000	76.4
matK-rps16_p3	matK-rps16	3	TCCTGAAGGAGTAGCGGATA	GTGCTCAACCTACAGGAACT	20	20	50.0	50.0	57.0	57.2	0.0	1.3	1.885909	3493	6130	2766-2795	1.000000	75.0
matK-rps16_p4	matK-rps16	4	TCCTGAAGGAGTAGCGGAT	GGTGCTCAACCTACAGGAAC	19	20	52.6	55.0	57.1	58.2	0.0	1.3	2.117045	3493	6130	2767-2796	1.000000	73.2
matK-rps16_p5	matK-rps16	5	CCTGAAGGAGTAGCGGATAT	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	55.9	58.2	0.0	1.3	2.285710	3493	6130	2766-2795	1.000000	71.8
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CGCCAAGCCCTGATTAATGA	AAAGGATTTGCAGTCCCCTG	20	20	50.0	50.0	58.3	58.1	0.0	14.6	0.391993	28469	29655	1326-1338	1.000000	82.4
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	CGCCAAGCCCTGATTAATGA	CGACACCCAGATTCGAACTG	20	20	50.0	55.0	58.3	58.6	0.0	16.2	0.970668	28469	29655	1351-1363	1.000000	77.7
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	ATGAAATTCTTCCGCCAAGC	AAAGGATTTGCAGTCCCCTG	20	20	45.0	50.0	57.1	58.1	0.0	14.6	1.018580	28469	29655	1338-1350	1.000000	77.4
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	CGCCAAGCCCTGATTAATGA	TTTGTTGATCAGGCGACACC	20	20	50.0	50.0	58.3	58.8	0.0	0.0	1.086040	28469	29655	1364-1376	1.000000	76.7
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	GTGGACATTCCCTCATTTCCA	AAAGGATTTGCAGTCCCCTG	21	20	47.6	50.0	57.9	58.1	0.0	14.6	1.157084	28469	29655	1262-1274	1.000000	76.4
trnC-GCA-petN_p1	trnC-GCA-petN	1	CCAGTTCGAATCTGGGTGTC	AGCCCAAGCGAGACTTACTA	20	20	55.0	50.0	58.3	58.2	26.3	0.0	0.424495	29727	30382	707-713	1.000000	87.8
trnC-GCA-petN_p2	trnC-GCA-petN	2	CAGTTCGAATCTGGGTGTCG	AGCCCAAGCGAGACTTACTA	20	20	55.0	50.0	58.6	58.2	15.7	0.0	0.801155	29727	30382	706-712	1.000000	84.8
trnC-GCA-petN_p3	trnC-GCA-petN	3	TAGGAAATTTGGCGGCATGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.9	58.2	14.3	0.0	1.040906	29727	30382	764-770	1.000000	82.3
trnC-GCA-petN_p4	trnC-GCA-petN	4	CCAGTTCGAATCTGGGTGTC	GCCCAAGCGAGACTTACTAT	20	20	55.0	50.0	58.3	57.1	26.3	0.0	1.165931	29727	30382	706-712	1.000000	81.9
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTAGGAAATTTGGCGGCATG	AGCCCAAGCGAGACTTACTA	20	20	45.0	50.0	57.0	58.2	14.3	0.0	1.108839	29727	30382	765-771	1.000000	81.8
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	CTTGCGCTTATTGCTACTGC	20	20	50.0	50.0	58.2	57.9	0.0	9.7	0.248147	30473	31684	1385-1782	1.000000	82.3
petN-psbM_p2	petN-psbM	2	TAGTAAGTCTCGCTTGGGCT	ATTGCTACTGCGCTGTTCAT	20	20	50.0	45.0	58.2	58.3	0.0	25.3	0.408587	30473	31684	1376-1773	1.000000	81.0
petN-psbM_p3	petN-psbM	3	TAGTAAGTCTCGCTTGGGCT	TATTGCTACTGCGCTGTTCA	20	20	50.0	45.0	58.2	57.6	0.0	25.3	0.529793	30473	31684	1377-1774	1.000000	80.0
petN-psbM_p4	petN-psbM	4	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCGCTGTTCATTT	20	20	50.0	45.0	58.2	58.5	0.0	25.3	0.628680	30473	31684	1374-1771	1.000000	79.2
petN-psbM_p5	petN-psbM	5	TAGTAAGTCTCGCTTGGGCT	TTGCTACTGCGCTGTTCATT	20	20	50.0	45.0	58.2	58.5	0.0	25.3	0.628680	30473	31684	1375-1772	1.000000	79.2
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	AGAGAGATGTCCTGGACCAC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	58.1	58.1	23.4	0.0	0.204766	33515	34057	611-663	1.000000	86.9
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	GAGAGATGTCCTGGACCACT	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	58.1	58.1	23.4	0.0	0.204766	33515	34057	610-662	1.000000	86.9
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	AGAGAGATGTCCTGGACCAC	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	58.1	58.1	23.4	0.0	0.226920	33515	34057	608-660	1.000000	86.7
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	GAGAGATGTCCTGGACCACT	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	58.1	58.1	23.4	0.0	0.226920	33515	34057	607-659	1.000000	86.7
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.233900	33515	34057	626-678	1.000000	86.5
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	AATTGAAGAAAACGGGCCCT	GGACCAGCCTACAAAAACGA	20	20	45.0	50.0	58.0	58.1	22.4	0.0	0.122795	34130	35219	1221-1279	1.000000	85.7
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	ATTGAAGAAAACGGGCCCTT	GGACCAGCCTACAAAAACGA	20	20	45.0	50.0	58.0	58.1	22.4	0.0	0.122795	34130	35219	1220-1278	1.000000	85.7
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	20	20	55.0	50.0	58.1	58.1	7.6	0.0	0.177181	34130	35219	1192-1250	1.000000	85.3
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.199336	34130	35219	1195-1253	1.000000	85.2
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	AACGGGCCCTTTTAACTCAG	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.1	58.1	22.4	0.0	0.207476	34130	35219	1211-1269	1.000000	85.0
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	48801	49571	821-821	1.000000	90.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	48801	49571	829-829	1.000000	90.4
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	48801	49571	853-853	1.000000	90.0
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	48801	49571	861-861	1.000000	89.5
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	ATCCAATCAAGTCCGTAGCG	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.357808	48801	49571	844-844	1.000000	89.1
trnM-CAU-atpE_p1	trnM-CAU-atpE	1	GGCTGGAGTCATTGGTTCAA	TAGGACACGAGTAGAGGCTG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.130118	54895	55082	249-263	1.000000	85.8
trnM-CAU-atpE_p2	trnM-CAU-atpE	2	GGCTGGAGTCATTGGTTCAA	CTCTCAGACGAGCTAGGACA	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.121559	54895	55082	262-276	1.000000	85.7
trnM-CAU-atpE_p3	trnM-CAU-atpE	3	GGCTGGAGTCATTGGTTCAA	GCGAATCTAGCTCTCAGACG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.121337	54895	55082	272-286	1.000000	85.6
trnM-CAU-atpE_p4	trnM-CAU-atpE	4	GGCTGGAGTCATTGGTTCAA	GCTGAGGGTAAGAGACAAGC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.107438	54895	55082	299-313	1.000000	85.5
trnM-CAU-atpE_p5	trnM-CAU-atpE	5	GGCTGGAGTCATTGGTTCAA	TGAGGCGAATCTAGCTCTCA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.148893	54895	55082	276-290	1.000000	85.4
atpB-rbcL_p1	atpB-rbcL	1	TGAGTGATACGCCCTAGGTT	GGTGACATAAGTCCCTCCCT	20	20	50.0	55.0	57.8	58.1	8.3	0.0	0.273626	56981	58105	1102-1228	1.000000	86.2
atpB-rbcL_p2	atpB-rbcL	2	TTGAGTGATACGCCCTAGGT	GGTGACATAAGTCCCTCCCT	20	20	50.0	55.0	57.8	58.1	8.3	0.0	0.273626	56981	58105	1103-1229	1.000000	86.2
atpB-rbcL_p3	atpB-rbcL	3	GGTGGAAAGGTGACATCGAG	GGTGACATAAGTCCCTCCCT	20	20	55.0	55.0	58.3	58.1	0.0	0.0	0.392206	56981	58105	1138-1264	1.000000	85.1
atpB-rbcL_p4	atpB-rbcL	4	TGAGTGATACGCCCTAGGTT	TGGTGACATAAGTCCCTCCC	20	20	50.0	55.0	57.8	58.4	8.3	0.0	0.574508	56981	58105	1103-1229	1.000000	83.8
atpB-rbcL_p5	atpB-rbcL	5	TTGAGTGATACGCCCTAGGT	TGGTGACATAAGTCCCTCCC	20	20	50.0	55.0	57.8	58.4	8.3	0.0	0.574508	56981	58105	1104-1230	1.000000	83.8
rbcL-accD_p1	rbcL-accD	1	GCAAATGGAGCCCTGAACTA	GGTTCTTCACTTCCACTGGT	20	20	50.0	50.0	57.9	57.4	0.0	0.0	0.769086	59570	60711	1353-1398	1.000000	75.9
rbcL-accD_p2	rbcL-accD	2	TAGCAAATGGAGCCCTGAAC	GGTTCTTCACTTCCACTGGT	20	20	50.0	50.0	57.9	57.4	0.0	0.0	0.769086	59570	60711	1355-1400	1.000000	75.9
rbcL-accD_p3	rbcL-accD	3	GCCGCTGCTTGTGAAATATG	GGTTCTTCACTTCCACTGGT	20	20	50.0	50.0	58.2	57.4	0.0	0.0	0.808973	59570	60711	1333-1378	1.000000	75.7
rbcL-accD_p4	rbcL-accD	4	TGAAGCTAGCAAATGGAGCC	GGTTCTTCACTTCCACTGGT	20	20	50.0	50.0	58.2	57.4	18.8	0.0	0.885136	59570	60711	1361-1406	1.000000	75.0
rbcL-accD_p5	rbcL-accD	5	TTTCGACCCAGTGGATAAGC	GGTTCTTCACTTCCACTGGT	20	20	50.0	50.0	57.6	57.4	12.0	0.0	1.036906	59570	60711	1295-1340	1.000000	73.9
petA-psbJ_p1	petA-psbJ	1	ATCCATTACGAGTCCAGGGT	TCTGGGTTGGGTTCATCTCT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.221040	65901	67289	1342-1532	1.000000	83.6
petA-psbJ_p2	petA-psbJ	2	GTTCTTCTTGGCATCCGTGA	TCTGGGTTGGGTTCATCTCT	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.236382	65901	67289	1317-1507	1.000000	83.5
petA-psbJ_p3	petA-psbJ	3	ATCCATTACGAGTCCAGGGT	CTGGGTTGGGTTCATCTCTG	20	20	50.0	55.0	57.8	57.9	0.0	0.0	0.296049	65901	67289	1341-1531	1.000000	83.0
petA-psbJ_p4	petA-psbJ	4	CGAGTCCAGGGTCTTTTGTT	TCTGGGTTGGGTTCATCTCT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.300910	65901	67289	1334-1524	1.000000	82.9
petA-psbJ_p5	petA-psbJ	5	GTTCTTCTTGGCATCCGTGA	CTGGGTTGGGTTCATCTCTG	20	20	50.0	55.0	58.2	57.9	0.0	0.0	0.311391	65901	67289	1316-1506	1.000000	82.9
clpP_p1	clpP	1	CGATCTTAACCTGCTGATGGT	AAAACGATGAAGTGAAGACA	21	20	47.6	35.0	57.8	53.1	0.0	0.0	6.098322	74083	76872	2155-2991	1.000000	45.2
clpP_p2	clpP	2	CGATCTTAACCTGCTGATGGT	AAACGATGAAGTGAAGACAA	21	20	47.6	35.0	57.8	53.1	0.0	0.0	6.098322	74083	76872	2154-2990	1.000000	45.2
clpP_p3	clpP	3	CGATCTTAACCTGCTGATGGT	AAAACGATGAAGTGAAGACAA	21	21	47.6	33.3	57.8	54.0	0.0	0.0	6.196225	74083	76872	2155-2991	1.000000	45.2
clpP_p4	clpP	4	CGATCTTAACCTGCTGATGGT	AACGATGAAGTGAAGACAAT	21	20	47.6	35.0	57.8	52.8	0.0	0.0	6.341627	74083	76872	2153-2989	1.000000	45.2
clpP_p5	clpP	5	CGATCTTAACCTGCTGATGGT	AAAAACGATGAAGTGAAGACA	21	21	47.6	33.3	57.8	54.0	0.0	0.0	6.196225	74083	76872	2156-2992	1.000000	45.1
psbH-petB_p1	psbH-petB	1	GTCGCAATGGCTCTATTTGC	TCCGGAATCTCTTCTTTGTGA	20	21	50.0	42.9	57.9	56.7	0.0	0.0	2.438930	80601	81323	796-871	1.000000	65.4
psbH-petB_p2	psbH-petB	2	GTCGCAATGGCTCTATTTGC	ACTTCTTGTAGCTTAGCCACT	20	21	50.0	42.9	57.9	56.7	0.0	4.3	2.449903	80601	81323	848-923	1.000000	65.3
psbH-petB_p3	psbH-petB	3	GGAGTCGCAATGGCTCTATT	TCCGGAATCTCTTCTTTGTGA	20	21	50.0	42.9	57.8	56.7	0.0	0.0	2.574608	80601	81323	799-874	1.000000	64.4
psbH-petB_p4	psbH-petB	4	GGAGTCGCAATGGCTCTATT	ACTTCTTGTAGCTTAGCCACT	20	21	50.0	42.9	57.8	56.7	0.0	4.3	2.585581	80601	81323	851-926	1.000000	64.2
psbH-petB_p5	psbH-petB	5	ACTTATGGGAGTCGCAATGG	TCCGGAATCTCTTCTTTGTGA	20	21	50.0	42.9	57.7	56.7	14.5	0.0	2.659952	80601	81323	806-881	1.000000	63.7
ndhF-rpl32_p1	ndhF-rpl32	1	ACTGGTAGCGGAAAAAGAGG	AGAAAAAGCCTTTACTGCTGC	20	21	50.0	42.9	57.5	57.3	8.0	0.0	2.124480	119856	120539	777-836	1.000000	68.6
ndhF-rpl32_p2	ndhF-rpl32	2	ACTGGTAGCGGAAAAAGAGG	GAAAAAGCCTTTACTGCTGCT	20	21	50.0	42.9	57.5	57.3	8.0	0.0	2.124480	119856	120539	776-835	1.000000	68.6
ndhF-rpl32_p3	ndhF-rpl32	3	CTGGTAGCGGAAAAAGAGGT	AGAAAAAGCCTTTACTGCTGC	20	21	50.0	42.9	57.5	57.3	0.0	0.0	2.124480	119856	120539	776-835	1.000000	68.6
ndhF-rpl32_p4	ndhF-rpl32	4	CTGGTAGCGGAAAAAGAGGT	GAAAAAGCCTTTACTGCTGCT	20	21	50.0	42.9	57.5	57.3	0.0	0.0	2.124480	119856	120539	775-834	1.000000	68.6
ndhF-rpl32_p5	ndhF-rpl32	5	CTGGTAGCGGAAAAAGAGGT	AAAAAGCCTTTACTGCTGCT	20	20	50.0	40.0	57.5	56.1	0.0	0.0	2.344534	119856	120539	774-833	1.000000	66.8
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AGCAGCAGTAAAGGCTTTTTC	TCCTAAGAGCAGCGTGTCTA	21	20	42.9	50.0	57.3	57.9	18.9	0.0	1.774348	120714	121510	870-933	1.000000	77.8
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GCAGCAGTAAAGGCTTTTTCT	TCCTAAGAGCAGCGTGTCTA	21	20	42.9	50.0	57.3	57.9	18.9	0.0	1.774348	120714	121510	869-932	1.000000	77.8
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGCAGCAGTAAAGGCTTTTTC	AGCAGCGTGTCTACCAATTT	21	20	42.9	45.0	57.3	57.8	18.9	0.0	1.835074	120714	121510	863-926	1.000000	77.3
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GCAGCAGTAAAGGCTTTTTCT	AGCAGCGTGTCTACCAATTT	21	20	42.9	45.0	57.3	57.8	18.9	0.0	1.835074	120714	121510	862-925	1.000000	77.3
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GCAGCAGTAAAGGCTTTTTCT	CTAAGAGCAGCGTGTCTACC	21	20	42.9	55.0	57.3	57.8	18.9	0.0	1.846365	120714	121510	867-930	1.000000	77.2
ndhH-rps15_p1	ndhH-rps15	1	AACACCATGCATTGATGGGT	TTCTGGGAAAACGTCAACGA	20	20	45.0	45.0	58.1	57.7	8.9	9.9	0.371345	130741	130877	319-329	1.000000	87.2
ndhH-rps15_p2	ndhH-rps15	2	AACACCATGCATTGATGGGT	CTGGGAAAACGTCAACGACT	20	20	45.0	50.0	58.1	58.4	8.9	9.9	0.480040	130741	130877	317-327	1.000000	86.4
ndhH-rps15_p3	ndhH-rps15	3	AACACCATGCATTGATGGGT	TCTGGGAAAACGTCAACGAC	20	20	45.0	50.0	58.1	58.4	8.9	9.9	0.484488	130741	130877	318-328	1.000000	86.3
ndhH-rps15_p4	ndhH-rps15	4	ATGCATTGATGGGTGGTGAG	TTCTGGGAAAACGTCAACGA	20	20	50.0	45.0	58.2	57.7	0.0	9.9	0.541158	130741	130877	313-323	1.000000	86.0
ndhH-rps15_p5	ndhH-rps15	5	CATTGATGGGTGGTGAGGAC	TTCTGGGAAAACGTCAACGA	20	20	55.0	45.0	58.2	57.7	0.0	9.9	0.557543	130741	130877	310-320	1.000000	85.9