primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-rps16_p1	trnK-rps16	1	AAAGCCGAGTACTCTACCGT	ACCTAATCAAACGAAATTCAAT	20	22	50.0	27.3	58.2	52.4	20.3	0.0	7.802566	4408	5313	957-965	1.000000	41.6
trnK-rps16_p2	trnK-rps16	2	AAGCCGAGTACTCTACCGTT	ACCTAATCAAACGAAATTCAAT	20	22	50.0	27.3	58.2	52.4	20.3	0.0	7.802566	4408	5313	956-964	1.000000	41.6
trnK-rps16_p3	trnK-rps16	3	TCTAGCCGCACTTAAAAGCC	ACCTAATCAAACGAAATTCAAT	20	22	50.0	27.3	58.3	52.4	0.0	0.0	7.899983	4408	5313	971-979	1.000000	41.6
trnK-rps16_p4	trnK-rps16	4	CCGCACTTAAAAGCCGAGTA	ACCTAATCAAACGAAATTCAAT	20	22	50.0	27.3	58.3	52.4	5.3	0.0	7.916952	4408	5313	966-974	1.000000	41.6
trnK-rps16_p5	trnK-rps16	5	AAGCCGAGTACTCTACCGTT	ACCTAATCAAACGAAATTCAATT	20	23	50.0	26.1	58.2	53.2	20.3	0.0	7.936638	4408	5313	956-964	1.000000	41.6
atpH-atpI_p1	atpH-atpI	1	AACAGAAGCGGCAGAAATCA	TCCCTGTCATGTTCCTTGGA	20	20	45.0	50.0	58.1	58.3	1.4	0.0	0.362752	14297	15365	1086-1203	1.000000	81.5
atpH-atpI_p2	atpH-atpI	2	TACCCTCTACAGCTTGACCC	TCCCTGTCATGTTCCTTGGA	20	20	55.0	50.0	58.1	58.3	0.0	0.0	0.396990	14297	15365	1171-1288	1.000000	81.2
atpH-atpI_p3	atpH-atpI	3	GCGATACCCTCTACAGCTTG	TCCCTGTCATGTTCCTTGGA	20	20	55.0	50.0	57.9	58.3	0.0	0.0	0.400507	14297	15365	1175-1292	1.000000	81.2
atpH-atpI_p4	atpH-atpI	4	CAGCAGCAATAACAGAAGCG	TCCCTGTCATGTTCCTTGGA	20	20	50.0	50.0	57.8	58.3	0.0	0.0	0.428635	14297	15365	1096-1213	1.000000	81.0
atpH-atpI_p5	atpH-atpI	5	GCCAATCCAGCAGCAATAAC	TCCCTGTCATGTTCCTTGGA	20	20	50.0	50.0	57.8	58.3	0.0	0.0	0.473359	14297	15365	1103-1220	1.000000	80.6
rps2-rpoC2_p1	rps2-rpoC2	1	AACTCCTGCCTCCATCATCT	TTCGAGGGGGAAATGAGAGA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.357147	17042	17249	377-386	1.000000	78.4
rps2-rpoC2_p2	rps2-rpoC2	2	TGCCTCCATCATCTCTTCCA	TTCGAGGGGGAAATGAGAGA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.364021	17042	17249	371-380	1.000000	78.4
rps2-rpoC2_p3	rps2-rpoC2	3	TCCTGCCTCCATCATCTCTT	TTCGAGGGGGAAATGAGAGA	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.434938	17042	17249	374-383	1.000000	77.8
rps2-rpoC2_p4	rps2-rpoC2	4	GAACTCCTGCCTCCATCATC	TTCGAGGGGGAAATGAGAGA	20	20	55.0	50.0	57.7	57.8	0.0	0.0	0.500676	17042	17249	378-387	1.000000	77.3
rps2-rpoC2_p5	rps2-rpoC2	5	CTCCTGCCTCCATCATCTCT	TTCGAGGGGGAAATGAGAGA	20	20	55.0	50.0	58.3	57.8	0.0	0.0	0.528170	17042	17249	375-384	1.000000	77.0
petN-psbM_p1	petN-psbM	1	ACTCGTAGTGTGGGGAAGAA	GCGACTGCACTATTCATTCT	20	20	50.0	45.0	58.0	55.9	0.0	0.0	2.059024	29754	30919	1275-1327	1.000000	64.3
petN-psbM_p2	petN-psbM	2	TCGTAGTGTGGGGAAGAAGT	GCGACTGCACTATTCATTCT	20	20	50.0	45.0	58.0	55.9	0.0	0.0	2.059024	29754	30919	1273-1325	1.000000	64.3
petN-psbM_p3	petN-psbM	3	CGTAGTGTGGGGAAGAAGTG	GCGACTGCACTATTCATTCT	20	20	55.0	45.0	57.9	55.9	0.0	0.0	2.133939	29754	30919	1272-1324	1.000000	63.7
petN-psbM_p4	petN-psbM	4	TAGTGTGGGGAAGAAGTGGA	GCGACTGCACTATTCATTCT	20	20	50.0	45.0	57.6	55.9	0.0	0.0	2.462717	29754	30919	1270-1322	1.000000	61.0
petN-psbM_p5	petN-psbM	5	CCTGGGCTGCTTTAATGGTA	GCGACTGCACTATTCATTCT	20	20	50.0	45.0	57.6	55.9	0.0	0.0	2.489026	29754	30919	1315-1367	1.000000	60.8
psbM-trnD(GUC)_p1	psbM-trnD(GUC)	1	CTGCCGATTATAGTCCGCTC	AGATCCCGCGAGTCTAATCA	20	20	55.0	50.0	58.0	58.0	9.3	8.2	0.033367	31025	31669	1379-1379	1.000000	80.7
psbM-trnD(GUC)_p2	psbM-trnD(GUC)	2	CTGCCGATTATAGTCCGCTC	CCTACGCTGGTTCAAATCCA	20	20	55.0	50.0	58.0	57.9	9.3	0.0	0.123978	31025	31669	1097-1097	1.000000	80.1
psbM-trnD(GUC)_p3	psbM-trnD(GUC)	3	TCATGGTTCGGGCGTTAAAA	AGATCCCGCGAGTCTAATCA	20	20	45.0	50.0	58.1	58.0	0.0	8.2	0.123795	31025	31669	1886-1934	1.000000	79.8
psbM-trnD(GUC)_p4	psbM-trnD(GUC)	4	GGTTCGGGCGTTAAAAATCG	AGATCCCGCGAGTCTAATCA	20	20	50.0	50.0	58.1	58.0	0.0	8.2	0.131303	31025	31669	1882-1930	1.000000	79.7
psbM-trnD(GUC)_p5	psbM-trnD(GUC)	5	GTTCGGGCGTTAAAAATCGG	AGATCCCGCGAGTCTAATCA	20	20	50.0	50.0	58.1	58.0	0.0	8.2	0.131303	31025	31669	1881-1929	1.000000	79.7
psbZ-trnG(GCC)_p1	psbZ-trnG(GCC)	1	TTGCTTCTCCTGATGGTTGG	AATCGAACCCGCATCTTCTC	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.263697	37528	37817	458-501	1.000000	79.8
psbZ-trnG(GCC)_p2	psbZ-trnG(GCC)	2	TTGCTTCTCCTGATGGTTGG	GAATCGAACCCGCATCTTCT	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.263697	37528	37817	459-502	1.000000	79.8
psbZ-trnG(GCC)_p3	psbZ-trnG(GCC)	3	GCTTCTCCTGATGGTTGGTC	AATCGAACCCGCATCTTCTC	20	20	55.0	50.0	58.3	58.1	0.0	0.0	0.314679	37528	37817	456-499	1.000000	79.4
psbZ-trnG(GCC)_p4	psbZ-trnG(GCC)	4	GCTTCTCCTGATGGTTGGTC	GAATCGAACCCGCATCTTCT	20	20	55.0	50.0	58.3	58.1	0.0	0.0	0.314679	37528	37817	457-500	1.000000	79.4
psbZ-trnG(GCC)_p5	psbZ-trnG(GCC)	5	GGGTTAGTCTTTCTGGTGGG	AATCGAACCCGCATCTTCTC	20	20	55.0	50.0	57.5	58.1	0.0	0.0	0.550350	37528	37817	393-436	1.000000	77.8
ycf3-trnS(GGA)_p1	ycf3-trnS(GGA)	1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	21	20	47.6	50.0	58.0	58.1	8.2	0.0	1.134847	45891	46674	887-887	1.000000	71.5
ycf3-trnS(GGA)_p2	ycf3-trnS(GGA)	2	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	21	20	47.6	50.0	58.0	58.2	8.2	0.0	1.191833	45891	46674	908-908	1.000000	71.0
ycf3-trnS(GGA)_p3	ycf3-trnS(GGA)	3	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	21	20	47.6	45.0	58.0	57.8	8.2	5.6	1.233148	45891	46674	858-858	1.000000	70.7
ycf3-trnS(GGA)_p4	ycf3-trnS(GGA)	4	CATTTATCCGCGATCTAGGCA	ACCCTCGGTAAACAAAAGCC	21	20	47.6	50.0	58.0	58.4	8.2	0.0	1.427808	45891	46674	888-888	1.000000	69.1
ycf3-trnS(GGA)_p5	ycf3-trnS(GGA)	5	TTTATCCGCGATCTAGGCAT	CCCTCGGTAAACAAAAGCCT	20	20	45.0	50.0	56.6	58.1	8.2	0.0	1.514239	45891	46674	885-885	1.000000	68.4
rbcL-accD_p1	rbcL-accD	1	GAGGCTAGCAAATGGAGTCC	ACCTGCGTTCTAACTCCTTC	20	20	55.0	50.0	58.0	57.3	18.2	0.0	0.785098	58113	58775	838-846	1.000000	74.2
rbcL-accD_p2	rbcL-accD	2	ATTTGAGTTTGCCGCAATGG	ACCTGCGTTCTAACTCCTTC	20	20	45.0	50.0	57.9	57.3	6.6	0.0	0.828681	58113	58775	779-787	1.000000	73.9
rbcL-accD_p3	rbcL-accD	3	GCTGCTTGTGAGGTATGGAA	ACCTGCGTTCTAACTCCTTC	20	20	50.0	50.0	57.9	57.3	0.0	0.0	0.859375	58113	58775	808-816	1.000000	73.6
rbcL-accD_p4	rbcL-accD	4	TGAGTTTGCCGCAATGGATA	ACCTGCGTTCTAACTCCTTC	20	20	45.0	50.0	57.9	57.3	6.6	0.0	0.866806	58113	58775	776-784	1.000000	73.6
rbcL-accD_p5	rbcL-accD	5	AGTTTGCCGCAATGGATACT	ACCTGCGTTCTAACTCCTTC	20	20	45.0	50.0	57.9	57.3	6.6	0.0	0.874285	58113	58775	774-782	1.000000	73.5
ycf4-cemA_p1	ycf4-cemA	1	CATTCCCTTGACCCGTACTG	GAGAAATCCACCAGGGCAAA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.100820	62048	62916	1044-1056	1.000000	79.7
ycf4-cemA_p2	ycf4-cemA	2	ATTCCCTTGACCCGTACTGA	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.129660	62048	62916	1043-1055	1.000000	79.5
ycf4-cemA_p3	ycf4-cemA	3	TTCCCTTGACCCGTACTGAT	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.129660	62048	62916	1042-1054	1.000000	79.5
ycf4-cemA_p4	ycf4-cemA	4	CTTGCGCGTACCAATTGAAG	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.1	58.1	8.4	0.0	0.194446	62048	62916	969-981	1.000000	79.0
ycf4-cemA_p5	ycf4-cemA	5	GAACAAAAAGCCGCCGAATT	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	58.2	58.1	0.0	0.0	0.298310	62048	62916	998-1010	1.000000	78.2
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AGTTAAAGCCGCTAGTAAAA	20	20	50.0	35.0	57.9	52.5	6.4	0.0	5.513522	66543	67725	1335-1335	1.000000	41.1
psbE-petL_p2	psbE-petL	2	ACTGACGAATAACCAACCCG	AGTTAAAGCCGCTAGTAAAA	20	20	50.0	35.0	57.9	52.5	0.0	0.0	5.551138	66543	67725	1348-1348	1.000000	41.1
psbE-petL_p3	psbE-petL	3	CAACCCGCAATGAATAGGGA	AGTTAAAGCCGCTAGTAAAAA	20	21	50.0	33.3	57.9	53.5	6.4	0.0	5.580161	66543	67725	1335-1335	1.000000	41.1
psbE-petL_p4	psbE-petL	4	ACTGACGAATAACCAACCCG	AGTTAAAGCCGCTAGTAAAAA	20	21	50.0	33.3	57.9	53.5	0.0	0.0	5.617776	66543	67725	1348-1348	1.000000	41.1
psbE-petL_p5	psbE-petL	5	AGAACGTTCTCCTGTGCTTC	AGTTAAAGCCGCTAGTAAAA	20	20	50.0	35.0	57.8	52.5	24.9	0.0	5.618531	66543	67725	1258-1258	1.000000	41.1
trnP(UGG)-psaJ_p1	trnP(UGG)-psaJ	1	ACCCGTGACATTTTGTACCC	TAGTACTGGTGCAACGGAGA	20	20	50.0	50.0	58.1	58.1	0.0	19.8	0.185977	68548	68948	489-506	1.000000	81.2
trnP(UGG)-psaJ_p2	trnP(UGG)-psaJ	2	CCCGTGACATTTTGTACCCA	TAGTACTGGTGCAACGGAGA	20	20	50.0	50.0	58.1	58.1	0.0	19.8	0.187320	68548	68948	488-505	1.000000	81.2
trnP(UGG)-psaJ_p3	trnP(UGG)-psaJ	3	ACAGGATTTGAACCCGTGAC	TAGTACTGGTGCAACGGAGA	20	20	50.0	50.0	58.1	58.1	0.0	19.8	0.194013	68548	68948	500-517	1.000000	81.1
trnP(UGG)-psaJ_p4	trnP(UGG)-psaJ	4	CAGGATTTGAACCCGTGACA	TAGTACTGGTGCAACGGAGA	20	20	50.0	50.0	58.1	58.1	0.0	19.8	0.195346	68548	68948	499-516	1.000000	81.1
trnP(UGG)-psaJ_p5	trnP(UGG)-psaJ	5	TGACAGGATTTGAACCCGTG	TAGTACTGGTGCAACGGAGA	20	20	50.0	50.0	58.1	58.1	0.0	19.8	0.195346	68548	68948	502-519	1.000000	81.1
ycf1_p1	ycf1	1	ACAGATGTCGTTTCTAGTCT	TCTTAACTGGGGCGGTAAGA	20	20	40.0	50.0	53.5	58.1	0.0	0.0	4.593854	110530	111693	1393-1393	1.000000	43.6
ycf1_p2	ycf1	2	ACAGATGTCGTTTCTAGTCT	AACATTCTTAACTGGGGCGG	20	20	40.0	50.0	53.5	58.2	0.0	0.0	4.701043	110530	111693	1398-1398	1.000000	42.7
ycf1_p3	ycf1	3	AACAGATGTCGTTTCTAGTCT	TCTTAACTGGGGCGGTAAGA	21	20	38.1	50.0	54.4	58.1	0.0	0.0	4.702885	110530	111693	1394-1394	1.000000	42.7
ycf1_p4	ycf1	4	AACAGATGTCGTTTCTAGTCT	AACATTCTTAACTGGGGCGG	21	20	38.1	50.0	54.4	58.2	0.0	0.0	4.810074	110530	111693	1399-1399	1.000000	41.9
ycf1_p5	ycf1	5	ACAGATGTCGTTTCTAGTCT	CTTAACTGGGGCGGTAAGAG	20	20	40.0	55.0	53.5	57.7	0.0	0.0	4.846355	110530	111693	1392-1392	1.000000	41.6
rpl32-trnL(UAG)_p1	rpl32-trnL(UAG)	1	TGGGACAGCGTTAAAAGCAT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.1	57.9	6.9	0.0	0.219990	114448	114989	669-681	1.000000	79.4
rpl32-trnL(UAG)_p2	rpl32-trnL(UAG)	2	TGGGACAGCGTTAAAAGCAT	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	58.1	57.8	6.9	0.0	0.280717	114448	114989	662-674	1.000000	79.0
rpl32-trnL(UAG)_p3	rpl32-trnL(UAG)	3	TGGGACAGCGTTAAAAGCAT	CCTAAGAGCAGCGTGTCTAC	20	20	45.0	55.0	58.1	57.8	6.9	0.0	0.292008	114448	114989	668-680	1.000000	78.9
rpl32-trnL(UAG)_p4	rpl32-trnL(UAG)	4	TGGGACAGCGTTAAAAGCAT	CTAAGAGCAGCGTGTCTACC	20	20	45.0	55.0	58.1	57.8	6.9	0.0	0.292008	114448	114989	667-679	1.000000	78.9
rpl32-trnL(UAG)_p5	rpl32-trnL(UAG)	5	TGGGACAGCGTTAAAAGCAT	GAGCAGCGTGTCTACCAATT	20	20	45.0	50.0	58.1	58.3	6.9	0.0	0.368860	114448	114989	663-675	1.000000	78.3
ccsA-ndhD_p1	ccsA-ndhD	1	TTGTGGCGTCTATTGGCTTT	TCAGTTGACAAGGTCGAAGT	20	20	45.0	45.0	58.1	56.7	0.0	0.0	1.366414	116132	116387	404-427	1.000000	72.0
ccsA-ndhD_p2	ccsA-ndhD	2	AATTGTGGCGTCTATTGGCT	TCAGTTGACAAGGTCGAAGT	20	20	45.0	45.0	57.9	56.7	0.0	0.0	1.399471	116132	116387	406-429	1.000000	71.7
ccsA-ndhD_p3	ccsA-ndhD	3	ATTGTGGCGTCTATTGGCTT	TCAGTTGACAAGGTCGAAGT	20	20	45.0	45.0	57.9	56.7	0.0	0.0	1.399471	116132	116387	405-428	1.000000	71.7
ccsA-ndhD_p4	ccsA-ndhD	4	TTGTGGCGTCTATTGGCTTT	AGGTATTGGTATGTACCCCGA	20	21	45.0	47.6	58.1	57.7	0.0	6.7	1.429027	116132	116387	441-464	1.000000	71.2
ccsA-ndhD_p5	ccsA-ndhD	5	ATTGTGGCGTCTATTGGCTT	AGGTATTGGTATGTACCCCGA	20	21	45.0	47.6	57.9	57.7	0.0	6.7	1.462084	116132	116387	442-465	1.000000	71.0
ndhD_p1	ndhD	1	AGTTGGGACCCAAAGGAAAC	TGGCCCTCTCTCATAAACCA	20	20	50.0	50.0	57.9	58.0	27.9	0.0	0.116726	116388	117890	2453-2476	1.000000	79.8
ndhD_p2	ndhD	2	GTTGGGACCCAAAGGAAACT	TGGCCCTCTCTCATAAACCA	20	20	50.0	50.0	57.9	58.0	27.9	0.0	0.116726	116388	117890	2452-2475	1.000000	79.8
ndhD_p3	ndhD	3	TTGTGGCGTCTATTGGCTTT	TGGCCCTCTCTCATAAACCA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.130299	116388	117890	2350-2373	1.000000	79.7
ndhD_p4	ndhD	4	AATTGTGGCGTCTATTGGCT	TGGCCCTCTCTCATAAACCA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.163356	116388	117890	2352-2375	1.000000	79.5
ndhD_p5	ndhD	5	ATTGTGGCGTCTATTGGCTT	TGGCCCTCTCTCATAAACCA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.163356	116388	117890	2351-2374	1.000000	79.5
ndhA_p1	ndhA	1	TGAACTCAGTTACCATAGGAAACA	CCCACCGGGTTTTATCAACT	24	20	37.5	50.0	57.6	57.8	0.0	6.3	4.637773	120459	122642	2409-2423	1.000000	43.1
ndhA_p2	ndhA	2	TCATGAACTCAGTTACCATAGGA	CCCACCGGGTTTTATCAACT	23	20	39.1	50.0	56.5	57.8	0.0	6.3	4.746193	120459	122642	2412-2426	1.000000	42.2
ndhA_p3	ndhA	3	TGTTTGAGACAAGTTGTGAA	CCCACCGGGTTTTATCAACT	20	20	35.0	50.0	53.2	57.8	14.6	6.3	5.009506	120459	122642	2341-2355	1.000000	40.2
ndhA_p4	ndhA	4	TTGTTTGAGACAAGTTGTGA	CCCACCGGGTTTTATCAACT	20	20	35.0	50.0	53.2	57.8	14.6	6.3	5.009506	120459	122642	2342-2356	1.000000	40.2
ndhA_p5	ndhA	5	TCATGAACTCAGTTACCATAGGAA	CCCACCGGGTTTTATCAACT	24	20	37.5	50.0	57.1	57.8	0.0	6.3	5.077209	120459	122642	2412-2426	1.000000	40.2
ycf1_p1	ycf1	1	GCTATCTCTATTCGCGCGAT	CAAATGTGCATTCCCCTCTT	20	20	50.0	45.0	57.8	56.3	35.2	0.0	1.908635	127796	127915	223-229	1.000000	68.6
ycf1_p2	ycf1	2	CTATTCGCGCGATCTCCTTT	CAAATGTGCATTCCCCTCTT	20	20	50.0	45.0	58.2	56.3	35.2	0.0	1.955027	127796	127915	216-222	1.000000	68.4
ycf1_p3	ycf1	3	TATTCGCGCGATCTCCTTTC	CAAATGTGCATTCCCCTCTT	20	20	50.0	45.0	58.2	56.3	35.2	0.0	1.959921	127796	127915	215-221	1.000000	68.3
ycf1_p4	ycf1	4	TCCCAGGTCTCTGCTATCTC	CAAATGTGCATTCCCCTCTT	20	20	55.0	45.0	57.6	56.3	0.0	0.0	2.100544	127796	127915	235-241	1.000000	66.9
ycf1_p5	ycf1	5	CCCAGGTCTCTGCTATCTCT	CAAATGTGCATTCCCCTCTT	20	20	55.0	45.0	57.6	56.3	0.0	0.0	2.107274	127796	127915	234-240	1.000000	66.8