primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	psbA-trnK-UUU	1	GCTTGTACTTTCGCGTCTCT	ACTAGTTCCGGGTTCGAGTC	20	20	50.0	55.0	58.3	58.8	5.8	0.0	1.128482	1149	1386	303-313	1.000000	78.8
psbA-trnK-UUU_p2	psbA-trnK-UUU	2	TAAAGACGGTTTTCGGTGCT	ACTAGTTCCGGGTTCGAGTC	20	20	45.0	55.0	57.8	58.8	0.0	0.0	1.071427	1149	1386	353-363	1.000000	78.1
psbA-trnK-UUU_p3	psbA-trnK-UUU	3	TAAAGACGGTTTTCGGTGCT	GACTAGTTCCGGGTTCGAGT	20	20	45.0	55.0	57.8	58.8	0.0	0.0	1.071427	1149	1386	354-364	1.000000	78.1
psbA-trnK-UUU_p4	psbA-trnK-UUU	4	AACACCGAACCATCCAATGT	ACTAGTTCCGGGTTCGAGTC	20	20	45.0	55.0	57.7	58.8	0.0	0.0	1.114189	1149	1386	372-382	1.000000	77.5
psbA-trnK-UUU_p5	psbA-trnK-UUU	5	AACACCGAACCATCCAATGT	GACTAGTTCCGGGTTCGAGT	20	20	45.0	55.0	57.7	58.8	0.0	0.0	1.114189	1149	1386	373-383	1.000000	77.4
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5751	7230	1460-1584	1.000000	86.7
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5751	7230	1471-1595	1.000000	86.1
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	20	20	45.0	55.0	58.1	57.8	8.1	16.9	0.350677	5751	7230	1464-1588	1.000000	85.6
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCATGTCCTTCAAGTCGCAC	CTCGGAGGTTCGAATCCTTC	20	20	50.0	55.0	58.2	57.8	0.0	16.9	0.415585	5751	7230	1475-1599	1.000000	85.0
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TACTCGGAGGTTCGAATCCT	20	20	45.0	50.0	58.1	57.6	8.1	16.1	0.571278	5751	7230	1466-1590	1.000000	83.8
psbK-psbI_p1	psbK-psbI	1	GTTTGGCAAGCTGCTGTAAG	ACGAAGAGTTTGAGAGTAAGCA	20	22	50.0	40.9	57.9	57.4	1.4	0.0	2.642049	7828	7992	218-226	1.000000	65.8
psbK-psbI_p2	psbK-psbI	2	TGGCAAGCTGCTGTAAGTTT	ACGAAGAGTTTGAGAGTAAGCA	20	22	45.0	40.9	58.3	57.4	1.4	0.0	2.894758	7828	7992	215-223	1.000000	63.9
psbK-psbI_p3	psbK-psbI	3	TTGGCAAGCTGCTGTAAGTT	ACGAAGAGTTTGAGAGTAAGCA	20	22	45.0	40.9	58.3	57.4	1.4	0.0	2.894758	7828	7992	216-224	1.000000	63.8
psbK-psbI_p4	psbK-psbI	4	TTTGGCAAGCTGCTGTAAGT	ACGAAGAGTTTGAGAGTAAGCA	20	22	45.0	40.9	58.3	57.4	1.4	0.0	2.894758	7828	7992	217-225	1.000000	63.8
psbK-psbI_p5	psbK-psbI	5	GTTTGGCAAGCTGCTGTAAG	AACGAAGAGTTTGAGAGTAAGCA	20	23	50.0	39.1	57.9	58.1	1.4	0.0	3.110849	7828	7992	219-227	1.000000	62.0
trnC-GCA-petN_p1	trnC-GCA-petN	1	GGGGGACTGCAAATCCTTTA	TTAAAGCAGCCCAAGCAAGA	20	20	50.0	45.0	57.5	58.0	14.7	0.0	0.531149	28419	29495	1156-1163	1.000000	87.8
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTTGGATCGAATTTGGCGG	TTAAAGCAGCCCAAGCAAGA	20	20	45.0	45.0	57.4	58.0	0.0	0.0	0.651774	28419	29495	1195-1202	1.000000	86.8
trnC-GCA-petN_p3	trnC-GCA-petN	3	GGGGGACTGCAAATCCTTTA	CAGCCCAAGCAAGACTTACT	20	20	50.0	50.0	57.5	57.8	14.7	0.0	0.716233	28419	29495	1150-1157	1.000000	86.3
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTTGGATCGAATTTGGCGG	CAGCCCAAGCAAGACTTACT	20	20	45.0	50.0	57.4	57.8	0.0	0.0	0.836858	28419	29495	1189-1196	1.000000	85.3
trnC-GCA-petN_p5	trnC-GCA-petN	5	CCAGTTCAAATCCGGGTGTC	TTAAAGCAGCCCAAGCAAGA	20	20	55.0	45.0	58.8	58.0	0.0	0.0	0.840774	28419	29495	1133-1140	1.000000	85.3
atpB-rbcL_p1	atpB-rbcL	1	GCCGACTGGAACACTTAGAG	GTCCACACAGTTGTCCATGT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.035012	56406	57317	1393-1455	1.000000	88.0
atpB-rbcL_p2	atpB-rbcL	2	GCCGACTGGAACACTTAGAG	AGATCCTCCAGACGTAGAGC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.026671	56406	57317	1594-1656	1.000000	87.4
atpB-rbcL_p3	atpB-rbcL	3	GCCGACTGGAACACTTAGAG	ATCCTCCAGACGTAGAGCTC	20	20	55.0	55.0	58.0	58.0	0.0	5.3	0.026671	56406	57317	1592-1654	1.000000	87.4
atpB-rbcL_p4	atpB-rbcL	4	GCCGACTGGAACACTTAGAG	GATCCTCCAGACGTAGAGCT	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.026671	56406	57317	1593-1655	1.000000	87.4
atpB-rbcL_p5	atpB-rbcL	5	GCCGACTGGAACACTTAGAG	CGGTCTCTCCAACGCATAAA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.006913	56406	57317	1831-1893	1.000000	86.6
rpl22-rps19_p1	rpl22-rps19	1	TGTTTCCTCATAAGAGCGCC	TCTGACTTTCGCGAGACATG	20	20	50.0	50.0	58.0	58.0	2.3	9.6	0.041563	86005	86056	223-238	1.000000	88.1
rpl22-rps19_p2	rpl22-rps19	2	TGTTTCCTCATAAGAGCGCC	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	58.0	58.0	2.3	22.4	0.048351	86005	86056	788-799	1.000000	83.0
rpl22-rps19_p3	rpl22-rps19	3	TCGAAGCCCGAGGATATTCT	TCTGACTTTCGCGAGACATG	20	20	50.0	50.0	58.0	58.0	0.0	9.6	0.020589	86005	86056	714-732	0.750000	74.7
rpl22-rps19_p4	rpl22-rps19	4	TCGAAGCCCGAGGATATTCT	TAGTAACTTGGTCTCGGGCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.075714	86005	86056	853-871	0.750000	73.6
rpl22-rps19_p5	rpl22-rps19	5	TCGAAGCCCGAGGATATTCT	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	58.0	58.0	0.0	22.4	0.027377	86005	86056	1275-1299	0.750000	73.1
trnH-GUG-rpl2_p1	trnH-GUG-rpl2	1	TGGATCAAGGCAGTGGATTG	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.157457	86543	86589	183-183	1.000000	80.9
trnH-GUG-rpl2_p2	trnH-GUG-rpl2	2	GGATCAAGGCAGTGGATTGT	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.158965	86543	86589	182-182	1.000000	80.9
trnH-GUG-rpl2_p3	trnH-GUG-rpl2	3	GTGGATCAAGGCAGTGGATT	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.158965	86543	86589	184-184	1.000000	80.9
trnH-GUG-rpl2_p4	trnH-GUG-rpl2	4	CGGATGTAGCCAAGTGGATC	GTTATCCTGCGCTTGGAAGA	20	20	55.0	50.0	58.1	58.0	0.2	22.4	0.153095	86543	86589	197-197	1.000000	80.8
trnH-GUG-rpl2_p5	trnH-GUG-rpl2	5	CGGATGTAGCCAAGTGGATC	TATCCTGCGCTTGGAAGAAG	20	20	55.0	50.0	58.1	57.7	0.2	22.4	0.444845	86543	86589	195-195	1.000000	78.5
ndhF_p1	ndhF	1	GGATCCACCGAACAAGACAA	TTTGTTCCTTTCGAAAGCGA	20	20	50.0	40.0	57.8	56.2	7.0	28.7	1.966349	112656	114881	2445-2505	0.750000	57.0
ndhF_p2	ndhF	2	ACCAAGTTCAACGTTAGCCA	TTTGTTCCTTTCGAAAGCGA	20	20	45.0	40.0	57.7	56.2	0.0	28.7	2.122753	112656	114881	2821-2890	0.750000	55.4
ndhF_p3	ndhF	3	ACAGGAACAAGAGGGATCCA	TTTGTTCCTTTCGAAAGCGA	20	20	50.0	40.0	58.0	56.2	15.2	28.7	1.832229	112656	114881	2458-2518	0.500000	47.5
ndhF_p4	ndhF	4	ACAGGAACAAGAGGGATCCA	TTTTGTTCCTTTCGAAAGCGA	20	21	50.0	38.1	58.0	57.0	15.2	28.7	2.084652	112656	114881	2459-2519	0.500000	45.5
ndhF_p5	ndhF	5	ACAGGAACAAGAGGGATCCA	GCGATAAGAATTGGTGAATCGG	20	22	50.0	45.5	58.0	57.9	15.2	0.0	2.178649	112656	114881	2442-2502	0.500000	44.7
ndhF-rpl32_p1	ndhF-rpl32	1	ACACTTGGAAAAGCCCACAT	GGAAGTTCTTTGAGCCATGGA	20	21	45.0	47.6	57.9	58.2	0.0	18.4	1.291281	114882	115830	1265-1289	0.750000	63.5
ndhF-rpl32_p2	ndhF-rpl32	2	GAAGAGGGAATTGTGCGGAT	GGAAGTTCTTTGAGCCATGGA	20	21	50.0	47.6	58.0	58.2	0.0	18.4	1.248793	114882	115830	1870-1894	0.750000	63.0
ndhF-rpl32_p3	ndhF-rpl32	3	TTTTTGACAAGCACTTGCCG	GGAAGTTCTTTGAGCCATGGA	20	21	45.0	47.6	58.1	58.2	10.3	18.4	1.284753	114882	115830	1662-1686	0.750000	62.7
ndhF-rpl32_p4	ndhF-rpl32	4	AGCAACTGCACCGACAAATA	GGAAGTTCTTTGAGCCATGGA	20	21	45.0	47.6	58.1	58.2	4.2	18.4	1.313795	114882	115830	1848-1872	0.750000	62.5
ndhF-rpl32_p5	ndhF-rpl32	5	TAGCAACTGCACCGACAAAT	GGAAGTTCTTTGAGCCATGGA	20	21	45.0	47.6	58.1	58.2	4.2	18.4	1.313795	114882	115830	1849-1873	0.750000	62.5
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TTTTTGTGCGACAAACAAGT	TCCTAAGAGCAGCGTGTCTA	20	20	35.0	50.0	55.2	57.9	12.1	0.0	2.967765	115990	116717	789-867	1.000000	64.3
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TTTTTGTGCGACAAACAAGT	AGCAGCGTGTCTACCAATTT	20	20	35.0	45.0	55.2	57.8	12.1	0.0	3.028492	115990	116717	782-860	1.000000	63.9
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TTTTTGTGCGACAAACAAGT	CCTAAGAGCAGCGTGTCTAC	20	20	35.0	55.0	55.2	57.8	12.1	0.0	3.039783	115990	116717	788-866	1.000000	63.8
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TTTTTGTGCGACAAACAAGT	CTAAGAGCAGCGTGTCTACC	20	20	35.0	55.0	55.2	57.8	12.1	0.0	3.039783	115990	116717	787-865	1.000000	63.8
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TTTTTGTGCGACAAACAAGT	GAGCAGCGTGTCTACCAATT	20	20	35.0	50.0	55.2	58.3	12.1	0.0	3.116634	115990	116717	783-861	1.000000	63.2
ccsA-ndhD_p1	ccsA-ndhD	1	GCCAAGTGCCATTTTTACCC	TGGGCCACGAGAACTATTTG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.201256	117842	118091	1036-1067	1.000000	82.8
ccsA-ndhD_p2	ccsA-ndhD	2	GCCAAGTGCCATTTTTACCC	GGGCCACGAGAACTATTTGT	20	20	50.0	50.0	57.9	57.9	0.0	4.4	0.202731	117842	118091	1035-1066	1.000000	82.8
ccsA-ndhD_p3	ccsA-ndhD	3	GCCAAGTGCCATTTTTACCC	TTGCATTGCCAGGAATGAGT	20	20	50.0	45.0	57.9	58.1	0.0	15.1	0.165819	117842	118091	1250-1281	1.000000	82.7
ccsA-ndhD_p4	ccsA-ndhD	4	GCCAAGTGCCATTTTTACCC	TGTCCCTGGTTGGTGATAGT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.132169	117842	118091	1578-1609	1.000000	82.6
ccsA-ndhD_p5	ccsA-ndhD	5	GCTAATGAGGCATGGGGATC	TGTCCCTGGTTGGTGATAGT	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.207283	117842	118091	1157-1188	1.000000	82.2
rps15-ycf1_p1	rps15-ycf1	1	AACACCATGCATTGATGGGT	ATCTCCCAGACGTCGTAGAG	20	20	45.0	55.0	58.1	58.0	8.9	19.5	0.102326	126281	126653	1135-1158	1.000000	85.2
rps15-ycf1_p2	rps15-ycf1	2	CCACCGTGTTACATAAGGCA	ATCTCCCAGACGTCGTAGAG	20	20	50.0	55.0	57.9	58.0	0.0	19.5	0.145773	126281	126653	1270-1293	1.000000	84.3
rps15-ycf1_p3	rps15-ycf1	3	TCCCGAATACCCAACTGACT	ATCTCCCAGACGTCGTAGAG	20	20	50.0	55.0	58.1	58.0	0.0	19.5	0.096719	126281	126653	931-941	0.875000	78.3
rps15-ycf1_p4	rps15-ycf1	4	TCCCGAATACCCAACTGACT	GGAGTGCACCCTTGAATCAA	20	20	50.0	50.0	58.1	58.1	0.0	14.6	0.143316	126281	126653	1340-1350	0.625000	66.6
rps15-ycf1_p5	rps15-ycf1	5	AACACCATGCATTGATGGGT	GGAGTGCACCCTTGAATCAA	20	20	45.0	50.0	58.1	58.1	8.9	14.6	0.148923	126281	126653	1557-1567	0.625000	66.4
rpl2-trnH-GUG_p1	rpl2-trnH-GUG	1	GTTATCCTGCGCTTGGAAGA	TGGATCAAGGCAGTGGATTG	20	20	50.0	50.0	58.0	57.9	22.4	0.0	0.157457	157175	157221	183-183	1.000000	80.9
rpl2-trnH-GUG_p2	rpl2-trnH-GUG	2	GTTATCCTGCGCTTGGAAGA	GGATCAAGGCAGTGGATTGT	20	20	50.0	50.0	58.0	57.9	22.4	0.0	0.158965	157175	157221	182-182	1.000000	80.9
rpl2-trnH-GUG_p3	rpl2-trnH-GUG	3	GTTATCCTGCGCTTGGAAGA	GTGGATCAAGGCAGTGGATT	20	20	50.0	50.0	58.0	57.9	22.4	0.0	0.158965	157175	157221	184-184	1.000000	80.9
rpl2-trnH-GUG_p4	rpl2-trnH-GUG	4	GTTATCCTGCGCTTGGAAGA	CGGATGTAGCCAAGTGGATC	20	20	50.0	55.0	58.0	58.1	22.4	0.2	0.153095	157175	157221	197-197	1.000000	80.8
rpl2-trnH-GUG_p5	rpl2-trnH-GUG	5	TATCCTGCGCTTGGAAGAAG	CGGATGTAGCCAAGTGGATC	20	20	50.0	55.0	57.7	58.1	22.4	0.2	0.444845	157175	157221	195-195	1.000000	78.5