primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnfM-CAU_p1	psbZ-trnfM-CAU	1	TTGCTTCTCCTGATGGTTGG	CGAGGCTCATAACCTTGAGG	20	20	50.0	55.0	57.8	57.8	0.0	16.0	0.436237	12191	12990	948-957	1.000000	78.4
psbZ-trnfM-CAU_p2	psbZ-trnfM-CAU	2	GCTTCTCCTGATGGTTGGTC	CGAGGCTCATAACCTTGAGG	20	20	55.0	55.0	58.3	57.8	0.0	16.0	0.487219	12191	12990	946-955	1.000000	78.0
psbZ-trnfM-CAU_p3	psbZ-trnfM-CAU	3	TTGCTTCTCCTGATGGTTGG	CAGTTTGGTAGCTCACGAGG	20	20	50.0	55.0	57.8	58.3	0.0	0.0	0.490228	12191	12990	963-972	1.000000	78.0
psbZ-trnfM-CAU_p4	psbZ-trnfM-CAU	4	GCTTCTCCTGATGGTTGGTC	CAGTTTGGTAGCTCACGAGG	20	20	55.0	55.0	58.3	58.3	0.0	0.0	0.541210	12191	12990	961-970	1.000000	77.6
psbZ-trnfM-CAU_p5	psbZ-trnfM-CAU	5	TTGCTTCTCCTGATGGTTGG	GAGCGGAGTAGAGCAGTTTG	20	20	50.0	55.0	57.8	58.4	0.0	0.0	0.570320	12191	12990	976-985	1.000000	77.3
trnG-UCC_p1	trnG-UCC	1	CAAACTGCTCTACTCCGCTC	AAGGAGCCGAATGAAATCCA	20	20	55.0	45.0	58.4	57.2	0.0	0.0	1.172243	13155	13202	238-238	1.000000	71.0
trnG-UCC_p2	trnG-UCC	2	CAAACTGCTCTACTCCGCTC	AGGAGCCGAATGAAATCCAA	20	20	55.0	45.0	58.4	57.2	0.0	0.0	1.172243	13155	13202	237-237	1.000000	71.0
trnG-UCC_p3	trnG-UCC	3	TGGTGGACGAAAAAGGTTGA	AAGGAGCCGAATGAAATCCA	20	20	45.0	45.0	57.6	57.2	0.0	0.0	1.228696	13155	13202	203-203	1.000000	70.6
trnG-UCC_p4	trnG-UCC	4	TGGTGGACGAAAAAGGTTGA	AGGAGCCGAATGAAATCCAA	20	20	45.0	45.0	57.6	57.2	0.0	0.0	1.228696	13155	13202	202-202	1.000000	70.6
trnG-UCC_p5	trnG-UCC	5	ACTGGTGGACGAAAAAGGTT	AGGAGCCGAATGAAATCCAA	20	20	45.0	45.0	57.6	57.2	0.0	0.0	1.237193	13155	13202	204-204	1.000000	70.5
trnG-UCC-trnT-GGU_p1	trnG-UCC-trnT-GGU	1	ACGAATCACACTTTTACCACT	CCATGGCATTACTCTACCACC	21	21	38.1	52.4	55.5	58.1	0.0	0.0	4.684647	13899	15911	2149-2169	1.000000	46.7
trnG-UCC-trnT-GGU_p2	trnG-UCC-trnT-GGU	2	ACGAATCACACTTTTACCACT	GACTTACGTCTTACCATGGCA	21	21	38.1	47.6	55.5	57.8	0.0	13.0	4.776909	13899	15911	2162-2182	1.000000	45.9
trnG-UCC-trnT-GGU_p3	trnG-UCC-trnT-GGU	3	ACGAATCACACTTTTACCACT	TGACTTACGTCTTACCATGGC	21	21	38.1	47.6	55.5	57.8	0.0	13.0	4.776909	13899	15911	2163-2183	1.000000	45.9
trnG-UCC-trnT-GGU_p4	trnG-UCC-trnT-GGU	4	AGAACGAATCACACTTTTACCAC	CCATGGCATTACTCTACCACC	23	21	39.1	52.4	57.3	58.1	0.0	0.0	4.817363	13899	15911	2152-2172	1.000000	45.6
trnG-UCC-trnT-GGU_p5	trnG-UCC-trnT-GGU	5	GAACGAATCACACTTTTACCACT	CCATGGCATTACTCTACCACC	23	21	39.1	52.4	57.3	58.1	0.0	0.0	4.817363	13899	15911	2151-2171	1.000000	45.6
psbM-petN_p1	psbM-petN	1	TGCTACTGCACTGTTCATTCT	TAGTAAGTCTCGCTTGGGCT	21	20	42.9	50.0	57.6	58.2	0.0	0.0	1.558772	18341	19076	898-898	1.000000	68.2
psbM-petN_p2	psbM-petN	2	TGCTACTGCACTGTTCATTCT	GTAGTATGGGGGAGGAGTGG	21	20	42.9	60.0	57.6	58.3	0.0	0.0	1.689144	18341	19076	842-842	1.000000	67.2
psbM-petN_p3	psbM-petN	3	TGCTACTGCACTGTTCATTCT	TAGTATGGGGGAGGAGTGGA	21	20	42.9	55.0	57.6	58.4	0.0	0.0	1.779576	18341	19076	841-841	1.000000	66.5
psbM-petN_p4	psbM-petN	4	TGCTACTGCACTGTTCATTC	TAGTAAGTCTCGCTTGGGCT	20	20	45.0	50.0	56.1	58.2	0.0	0.0	2.081320	18341	19076	898-898	1.000000	64.1
psbM-petN_p5	psbM-petN	5	TGCTACTGCACTGTTCATTCT	AGTAGTATGGGGGAGGAGTG	21	20	42.9	55.0	57.6	57.2	0.0	0.0	2.184882	18341	19076	843-843	1.000000	63.3
trnC-GCA-rpoB_p1	trnC-GCA-rpoB	1	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	20	20	50.0	55.0	58.1	58.3	14.6	0.0	0.395580	20144	21302	1243-1254	1.000000	77.9
trnC-GCA-rpoB_p2	trnC-GCA-rpoB	2	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	20	20	50.0	55.0	58.1	58.3	14.6	0.0	0.395580	20144	21302	1242-1253	1.000000	77.9
trnC-GCA-rpoB_p3	trnC-GCA-rpoB	3	AAAGGATTTGCAGTCCCCTG	AAGTTCTTCTGCCAAGCCTT	20	20	50.0	45.0	58.1	57.6	14.6	0.0	0.442790	20144	21302	1307-1318	1.000000	77.4
trnC-GCA-rpoB_p4	trnC-GCA-rpoB	4	AAAGGATTTGCAGTCCCCTG	AGTTCTTCTGCCAAGCCTTG	20	20	50.0	50.0	58.1	58.4	14.6	0.0	0.457296	20144	21302	1306-1317	1.000000	77.3
trnC-GCA-rpoB_p5	trnC-GCA-rpoB	5	AAAGGATTTGCAGTCCCCTG	CAAGTTCTTCTGCCAAGCCT	20	20	50.0	50.0	58.1	58.4	14.6	0.0	0.457296	20144	21302	1308-1319	1.000000	77.3
atpI-atpH_p1	atpI-atpH	1	TAGCCGCAGCCTATATAGGT	GCAGTACCTTGACCAACTCC	20	20	50.0	55.0	57.5	58.2	26.3	0.0	0.717204	33366	34186	955-957	1.000000	74.8
atpI-atpH_p2	atpI-atpH	2	TAGCCGCAGCCTATATAGGT	AAGCAGCAGCAATTAGTGGA	20	20	50.0	45.0	57.5	57.8	26.3	3.2	0.731910	33366	34186	888-890	1.000000	74.8
atpI-atpH_p3	atpI-atpH	3	TAGCCGCAGCCTATATAGGT	GCCAATCCAGCAGCAATAAC	20	20	50.0	50.0	57.5	57.8	26.3	0.0	0.741275	33366	34186	910-912	1.000000	74.7
atpI-atpH_p4	atpI-atpH	4	TAGCCGCAGCCTATATAGGT	ATAACGGAAGCAGCAGCAAT	20	20	50.0	45.0	57.5	58.2	26.3	3.2	0.771708	33366	34186	895-897	1.000000	74.5
atpI-atpH_p5	atpI-atpH	5	ATTTTTGCAACGTTAGCCGC	GCAGTACCTTGACCAACTCC	20	20	45.0	55.0	58.6	58.2	16.5	0.0	0.775939	33366	34186	968-970	1.000000	74.4
psaA-ycf3_p1	psaA-ycf3	1	TGTTCTTGAGAAATGGCCGG	TATAGCGCTTACTCCGGGAA	20	20	50.0	50.0	58.5	58.0	0.0	18.7	0.480902	43439	44054	796-797	1.000000	76.8
psaA-ycf3_p2	psaA-ycf3	2	TTTTACTTCTGGTTCCGGCG	TATAGCGCTTACTCCGGGAA	20	20	50.0	50.0	58.5	58.0	0.0	18.7	0.499445	43439	44054	724-725	1.000000	76.7
psaA-ycf3_p3	psaA-ycf3	3	TGTTCTTGAGAAATGGCCGG	GCGCTTACTCCGGGAAATTA	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.522343	43439	44054	792-793	1.000000	76.4
psaA-ycf3_p4	psaA-ycf3	4	TTTTACTTCTGGTTCCGGCG	GCGCTTACTCCGGGAAATTA	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.540887	43439	44054	720-721	1.000000	76.3
psaA-ycf3_p5	psaA-ycf3	5	GGTTTGGCCCATTCCTCAAA	TATAGCGCTTACTCCGGGAA	20	20	50.0	50.0	58.7	58.0	0.0	18.7	0.667465	43439	44054	777-778	1.000000	75.3
rbcL-psaI_p1	rbcL-psaI	1	CGCAGCTTGTGAAGTATGGA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.3	58.0	0.0	26.9	0.301363	58173	59420	1384-1385	1.000000	78.6
rbcL-psaI_p2	rbcL-psaI	2	CGCAGCTTGTGAAGTATGGA	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.3	57.9	0.0	26.8	0.377897	58173	59420	1390-1391	1.000000	78.0
rbcL-psaI_p3	rbcL-psaI	3	GGCGATCAAATTCGAGTTCG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.6	58.0	0.0	26.9	0.380261	58173	59420	1363-1364	1.000000	78.0
rbcL-psaI_p4	rbcL-psaI	4	TGGAAGGCGATCAAATTCGA	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	57.6	58.0	0.0	26.9	0.421933	58173	59420	1368-1369	1.000000	77.6
rbcL-psaI_p5	rbcL-psaI	5	GGCGATCAAATTCGAGTTCG	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	57.6	57.9	0.0	26.8	0.456795	58173	59420	1369-1370	1.000000	77.3
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	AAAACAAACGCGCTACCAAG	AAACCTGCTAAAGCCCCAAA	20	20	45.0	45.0	57.9	57.9	5.7	0.0	0.209515	66276	66663	498-498	1.000000	79.3
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CCTCGCTACTGCCCATAAAG	AAACCTGCTAAAGCCCCAAA	20	20	55.0	45.0	58.1	57.9	0.0	0.0	0.224524	66276	66663	574-574	1.000000	79.0
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	CAAAACAAACGCGCTACCAA	AAACCTGCTAAAGCCCCAAA	20	20	45.0	45.0	58.2	57.9	5.7	0.0	0.266772	66276	66663	499-499	1.000000	78.8
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	TTGTACCCAAAACAAACGCG	AAACCTGCTAAAGCCCCAAA	20	20	45.0	45.0	57.8	57.9	3.5	0.0	0.285929	66276	66663	506-506	1.000000	78.7
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	TACCCAAAACAAACGCGCTA	AAACCTGCTAAAGCCCCAAA	20	20	45.0	45.0	58.4	57.9	5.7	0.0	0.504672	66276	66663	503-503	1.000000	76.9
psbT-psbN_p1	psbT-psbN	1	TTCCGAGAACCACCTAAGGT	ATCCATTCGAAGAACACGGG	20	20	50.0	50.0	58.0	58.0	0.0	3.3	0.043983	72698	72745	116-116	1.000000	83.1
psbT-psbN_p2	psbT-psbN	2	TCTTCTTCCGAGAACCACCT	ATCCATTCGAAGAACACGGG	20	20	50.0	50.0	58.0	58.0	0.0	3.3	0.035298	72698	72745	121-121	1.000000	83.0
psbT-psbN_p3	psbT-psbN	3	TCTTCTTCCGAGAACCACCT	TCGCCATCTCCATATCTGGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.026579	72698	72745	208-208	1.000000	81.7
psbT-psbN_p4	psbT-psbN	4	TTCCGAGAACCACCTAAGGT	TCGCCATCTCCATATCTGGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.035264	72698	72745	203-203	1.000000	81.7
psbT-psbN_p5	psbT-psbN	5	TCTTCTTCCGAGAACCACCT	GTCGCCATCTCCATATCTGG	20	20	50.0	55.0	58.0	57.6	0.0	0.0	0.387930	72698	72745	209-209	1.000000	78.8
petD_p1	petD	1	ACGAAAGAAAATAAGGCGGA	TGAATGAATCAACCTGAAAAATCC	20	24	40.0	33.3	55.4	56.2	0.0	0.0	8.468052	74994	76228	1277-1281	1.000000	40.4
petD_p2	petD	2	ACGAAAGAAAATAAGGCGGA	TTGAATGAATCAACCTGAAAAATCC	20	25	40.0	32.0	55.4	56.8	0.0	0.0	8.823762	74994	76228	1278-1282	1.000000	40.4
petD_p3	petD	3	ACGAAAGAAAATAAGGCGGAT	TGAATGAATCAACCTGAAAAATCC	21	24	38.1	33.3	56.0	56.2	0.0	0.0	8.893446	74994	76228	1277-1281	1.000000	40.4
petD_p4	petD	4	ACGAAAGAAAATAAGGCGGA	GAATGAATCAACCTGAAAAATCC	20	23	40.0	34.8	55.4	54.5	0.0	0.0	9.107837	74994	76228	1276-1280	1.000000	40.4
petD_p5	petD	5	ACGAAAGAAAATAAGGCGGATT	TGAATGAATCAACCTGAAAAATCC	22	24	36.4	33.3	56.7	56.2	0.0	0.0	9.157018	74994	76228	1277-1281	1.000000	40.4
infA_p1	infA	1	CGCAAACCAATACGCACTTT	TTAAAGCGTATCAGCCGACC	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.114321	78351	78674	1058-1074	1.000000	80.0
infA_p2	infA	2	TCCACTACCAGCACCCTTTA	TTAAAGCGTATCAGCCGACC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.102397	78351	78674	1320-1336	1.000000	79.9
infA_p3	infA	3	TTGCAAACCCACTGTACGAA	TTAAAGCGTATCAGCCGACC	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.109251	78351	78674	1284-1300	1.000000	79.9
infA_p4	infA	4	GTAATGCGGCATCTCTTCCA	TTAAAGCGTATCAGCCGACC	20	20	50.0	50.0	58.0	58.1	17.0	0.0	0.115874	78351	78674	1336-1352	1.000000	79.8
infA_p5	infA	5	TAATGCGGCATCTCTTCCAC	TTAAAGCGTATCAGCCGACC	20	20	50.0	50.0	58.0	58.1	17.0	0.0	0.115874	78351	78674	1335-1351	1.000000	79.8
infA-rps8_p1	infA-rps8	1	TGACTTTTGCTTCTCTCGGG	TTTTGGGCGGAATGGGAATT	20	20	50.0	45.0	57.8	58.3	0.0	0.0	0.519304	78675	78786	277-277	1.000000	77.9
infA-rps8_p2	infA-rps8	2	GACTTTTGCTTCTCTCGGGT	TTTTGGGCGGAATGGGAATT	20	20	50.0	45.0	57.8	58.3	0.0	0.0	0.520813	78675	78786	276-276	1.000000	77.9
infA-rps8_p3	infA-rps8	3	TGACTTTTGCTTCTCTCGGG	GGCGGAATGGGAATTGCTAT	20	20	50.0	50.0	57.8	58.4	0.0	0.0	0.549010	78675	78786	272-272	1.000000	77.7
infA-rps8_p4	infA-rps8	4	GACTTTTGCTTCTCTCGGGT	GGCGGAATGGGAATTGCTAT	20	20	50.0	50.0	57.8	58.4	0.0	0.0	0.550518	78675	78786	271-271	1.000000	77.7
infA-rps8_p5	infA-rps8	5	GGGTAGGGCTTCCGTAACTA	TTTTGGGCGGAATGGGAATT	20	20	55.0	45.0	58.2	58.3	1.4	0.0	0.568969	78675	78786	309-309	1.000000	77.3
rps3-rpl22_p1	rps3-rpl22	1	GTGCGAACCAAAAGGAATGG	AAATTCAGACCTCGAGCTCG	20	20	50.0	50.0	57.9	57.7	0.0	30.0	0.350400	82198	82257	228-229	1.000000	78.2
rps3-rpl22_p2	rps3-rpl22	2	ACCAAGTCTGAAACCGAGTG	AAATTCAGACCTCGAGCTCG	20	20	50.0	50.0	57.8	57.7	0.0	30.0	0.526320	82198	82257	194-195	1.000000	77.0
rps3-rpl22_p3	rps3-rpl22	3	CCAAGTCTGAAACCGAGTGG	AAATTCAGACCTCGAGCTCG	20	20	55.0	50.0	58.5	57.7	0.0	30.0	0.772901	82198	82257	193-194	1.000000	75.1
rps3-rpl22_p4	rps3-rpl22	4	CAAGTCTGAAACCGAGTGGA	AAATTCAGACCTCGAGCTCG	20	20	50.0	50.0	57.5	57.7	0.0	30.0	0.812130	82198	82257	192-193	1.000000	74.7
rps3-rpl22_p5	rps3-rpl22	5	GTTGTGCGAACCAAAAGGAA	AAATTCAGACCTCGAGCTCG	20	20	45.0	50.0	57.4	57.7	0.0	30.0	0.856269	82198	82257	231-232	1.000000	74.2
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AAGATCATTTTCCAGCGGCA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.2	57.9	1.4	0.0	0.286165	107925	108658	839-840	1.000000	78.4
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGGGTTTTTCTGGGCAAACA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.251505	107925	108658	802-802	0.500000	58.0
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGGGTTTTTCTGGGCAAACA	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	58.1	57.8	0.0	0.0	0.312231	107925	108658	795-795	0.500000	57.5
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGGGTTTTTCTGGGCAAACA	GCTTGAGCACTGCTTCCTAA	20	20	45.0	50.0	58.1	58.2	0.0	4.7	0.313714	107925	108658	816-816	0.500000	57.5
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGGGTTTTTCTGGGCAAACA	CTAAGAGCAGCGTGTCTACC	20	20	45.0	55.0	58.1	57.8	0.0	0.0	0.323522	107925	108658	800-800	0.500000	57.4
ndhG-ndhI_p1	ndhG-ndhI	1	CCCAGCGAAAAGGCAGAATA	GAGTCGGTTACCAATCTCCA	20	20	50.0	50.0	58.2	56.7	0.0	1.1	1.591617	113431	113668	475-475	1.000000	69.3
ndhG-ndhI_p2	ndhG-ndhI	2	CCCAGCGAAAAGGCAGAATA	TGAGTCGGTTACCAATCTCC	20	20	50.0	50.0	58.2	56.7	0.0	1.1	1.591617	113431	113668	476-476	1.000000	69.3
ndhG-ndhI_p3	ndhG-ndhI	3	CCCAGCGAAAAGGCAGAATA	TGAGTCGGTTACCAATCTCCA	20	21	50.0	47.6	58.2	58.5	0.0	1.1	1.718036	113431	113668	476-476	1.000000	68.3
ndhG-ndhI_p4	ndhG-ndhI	4	CCCAGCGAAAAGGCAGAATA	TTGAGTCGGTTACCAATCTCC	20	21	50.0	47.6	58.2	57.4	0.0	1.1	1.854682	113431	113668	477-477	1.000000	67.2
ndhG-ndhI_p5	ndhG-ndhI	5	CCCAGCGAAAAGGCAGAATA	GAGTCGGTTACCAATCTCCAT	20	21	50.0	47.6	58.2	57.2	0.0	1.1	2.071228	113431	113668	475-475	1.000000	65.5