primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	GTCTTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.081289	7021	7791	845-851	1.000000	80.6
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	ATGTCTTTCAAGTCGCACGT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	0.0	16.9	0.210236	7021	7791	847-853	1.000000	79.5
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GCACGTTGCTTTTTACCACA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.8	58.1	0.0	16.9	0.272396	7021	7791	833-839	1.000000	79.1
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CATGTCTTTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.8	58.1	0.0	16.9	0.293454	7021	7791	848-854	1.000000	78.9
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TGTCTTTCAAGTCGCACGTT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.4	58.1	0.0	16.9	0.428755	7021	7791	846-852	1.000000	77.8
trnQ-UUG-psbK_p1	trnQ-UUG-psbK	1	GATATGCTCTGGGACGGAAG	AGGCTTCAGGTAATTTCACGA	20	21	55.0	42.9	57.6	57.3	0.0	0.0	2.143074	7864	8597	901-902	1.000000	63.3
trnQ-UUG-psbK_p2	trnQ-UUG-psbK	2	GACCAAAACCCGTTGCCTTA	AGGCTTCAGGTAATTTCACGA	20	21	50.0	42.9	58.7	57.3	0.0	0.0	2.375323	7864	8597	859-860	1.000000	61.5
trnQ-UUG-psbK_p3	trnQ-UUG-psbK	3	GATATGCTCTGGGACGGAAG	AGGCTTCAGGTAATTTCACGAA	20	22	55.0	40.9	57.6	58.0	0.0	0.0	2.463116	7864	8597	901-902	1.000000	60.7
trnQ-UUG-psbK_p4	trnQ-UUG-psbK	4	AATGGATATGCTCTGGGACG	AGGCTTCAGGTAATTTCACGA	20	21	50.0	42.9	57.2	57.3	0.0	0.0	2.543157	7864	8597	905-906	1.000000	60.1
trnQ-UUG-psbK_p5	trnQ-UUG-psbK	5	GAATAGCGGGACCAAAACCC	AGGCTTCAGGTAATTTCACGA	20	21	55.0	42.9	58.9	57.3	0.0	0.0	2.595320	7864	8597	868-869	1.000000	59.7
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	CAATCCGACGCTTTAGTCCA	TGAATCAAACGAGGGATCCC	20	20	50.0	50.0	58.0	57.3	0.0	24.6	0.714678	9420	10611	1244-1311	1.000000	79.1
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	AATCCGACGCTTTAGTCCAC	TGAATCAAACGAGGGATCCC	20	20	50.0	50.0	58.0	57.3	0.0	24.6	0.716066	9420	10611	1243-1310	1.000000	79.1
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	ACGGAAAGAGAGGGATTCGA	TGAATCAAACGAGGGATCCC	20	20	50.0	50.0	58.2	57.3	0.0	24.6	0.861444	9420	10611	1298-1365	1.000000	77.7
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	TGGAAACGGAAAGAGAGGGA	TGAATCAAACGAGGGATCCC	20	20	50.0	50.0	58.3	57.3	0.0	24.6	0.985551	9420	10611	1303-1370	1.000000	76.7
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	CAATCCGACGCTTTAGTCCA	GGATCCATAGAACGAATCGCA	20	21	50.0	47.6	58.0	58.0	0.0	7.0	1.031212	9420	10611	1212-1279	1.000000	76.6
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	GGTTCAAATCCTATTGGACGC	AAGACATTCACTCGGGAAGC	21	20	47.6	50.0	57.3	57.9	0.0	0.0	1.793395	11803	12293	587-611	1.000000	69.1
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	GGTTCAAATCCTATTGGACGC	CCAAGACATTCACTCGGGAA	21	20	47.6	50.0	57.3	57.5	0.0	0.0	2.165486	11803	12293	589-613	1.000000	66.2
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	TGGATAGGACAGAGGTCTTCT	AAGACATTCACTCGGGAAGC	21	20	47.6	50.0	56.9	57.9	0.0	0.0	2.152209	11803	12293	622-646	1.000000	66.1
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	GGTTCAAATCCTATTGGACGC	ACCAAGACATTCACTCGGGA	21	20	47.6	50.0	57.3	58.7	0.0	0.0	2.344727	11803	12293	590-614	1.000000	64.7
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	GTTCAAATCCTATTGGACGCA	AAGACATTCACTCGGGAAGC	21	20	42.9	50.0	56.6	57.9	0.0	0.0	2.496339	11803	12293	586-610	1.000000	63.5
atpF-atpH_p1	atpF-atpH	1	ATTAAACCCGAAACTCCCGG	TGGGCTGGTTGTAGCATTAG	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.536681	15172	15773	719-739	1.000000	76.3
atpF-atpH_p2	atpF-atpH	2	ATTAAACCCGAAACTCCCGG	AGGCAGAGGGAAAAATACGG	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.538372	15172	15773	786-806	1.000000	76.2
atpF-atpH_p3	atpF-atpH	3	ATTAAACCCGAAACTCCCGG	GCAGAGGGAAAAATACGGGG	20	20	50.0	55.0	57.9	58.6	0.0	0.0	0.728758	15172	15773	784-804	1.000000	74.7
atpF-atpH_p4	atpF-atpH	4	ATTAAACCCGAAACTCCCGG	GGCAGAGGGAAAAATACGGG	20	20	50.0	55.0	57.9	58.6	0.0	0.0	0.728758	15172	15773	785-805	1.000000	74.7
atpF-atpH_p5	atpF-atpH	5	ATTAAACCCGAAACTCCCGG	CCGAGGCAGAGGGAAAAATA	20	20	50.0	50.0	57.9	57.3	0.0	0.0	0.828409	15172	15773	789-809	1.000000	73.9
rpoB_p1	rpoB	1	TGGCATTTTCTTTTGACCCG	AAAGCAAGGATATGGGCTCG	20	20	45.0	50.0	57.2	58.0	0.0	0.0	0.837567	29204	29323	175-176	1.000000	76.0
rpoB_p2	rpoB	2	GGCATTTTCTTTTGACCCGA	AAAGCAAGGATATGGGCTCG	20	20	45.0	50.0	56.9	58.0	0.0	0.0	1.125785	29204	29323	174-175	1.000000	73.7
rpoB_p3	rpoB	3	ATGGCATTTTCTTTTGACCCG	AAAGCAAGGATATGGGCTCG	21	20	42.9	50.0	57.7	58.0	0.0	0.0	1.344731	29204	29323	176-177	1.000000	72.0
rpoB_p4	rpoB	4	CGCCCACACAAGCAAATTG	AAAGCAAGGATATGGGCTCG	19	20	52.6	50.0	58.5	58.0	0.0	0.0	1.507302	29204	29323	211-212	1.000000	70.2
rpoB_p5	rpoB	5	GGCATTTTCTTTTGACCCGAT	AAAGCAAGGATATGGGCTCG	21	20	42.9	50.0	57.4	58.0	0.0	0.0	1.620026	29204	29323	174-175	1.000000	69.8
psaA-ycf3_p1	psaA-ycf3	1	CCCATTCCTCGAAAGACGTT	AGCCCTTACCCCTGGTAATT	20	20	50.0	50.0	57.9	58.0	0.0	6.2	0.098894	47189	48030	943-988	1.000000	82.9
psaA-ycf3_p2	psaA-ycf3	2	CCCATTCCTCGAAAGACGTT	AGCTATAGCCCTTACCCCTG	20	20	50.0	55.0	57.9	58.0	0.0	21.2	0.126257	47189	48030	949-994	1.000000	82.6
psaA-ycf3_p3	psaA-ycf3	3	AGAAATGACCGGGTTTAGCC	AGCCCTTACCCCTGGTAATT	20	20	50.0	50.0	57.9	58.0	6.3	6.2	0.128409	47189	48030	961-1006	1.000000	82.6
psaA-ycf3_p4	psaA-ycf3	4	CCCATTCCTCGAAAGACGTT	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.135721	47189	48030	974-1019	1.000000	82.5
psaA-ycf3_p5	psaA-ycf3	5	AGAAATGACCGGGTTTAGCC	AGCTATAGCCCTTACCCCTG	20	20	50.0	55.0	57.9	58.0	6.3	21.2	0.155772	47189	48030	967-1012	1.000000	82.3
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	51665	53038	1434-1456	1.000000	79.7
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	51665	53038	1442-1464	1.000000	79.2
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	AAATGCGACGCTCTAACCTC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.4	58.0	22.1	0.0	0.363047	51665	53038	1406-1428	1.000000	78.8
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	AAATGCGACGCTCTAACCTC	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.4	58.1	22.1	0.0	0.427856	51665	53038	1414-1436	1.000000	78.3
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	AGTCCGTAGCGTCTACCAAT	20	20	50.0	50.0	57.8	58.2	15.5	10.2	0.480302	51665	53038	1448-1470	1.000000	77.8
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TGGCCCTTTATTAATCGGGG	AAAAGATGCACGTTGGGTCT	20	20	50.0	45.0	57.3	58.0	15.4	0.0	0.686887	56543	57625	1250-1302	1.000000	79.1
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TGGCCCTTTATTAATCGGGG	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.3	57.8	15.4	0.0	0.865427	56543	57625	1179-1231	1.000000	77.7
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TGGCCCTTTATTAATCGGGG	GATGCACGTTGGGTCTGTAA	20	20	50.0	50.0	57.3	58.2	15.4	0.0	0.864810	56543	57625	1246-1298	1.000000	77.6
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TGGCCCTTTATTAATCGGGG	ATGCACGTTGGGTCTGTAAA	20	20	50.0	45.0	57.3	57.7	15.4	0.0	0.932149	56543	57625	1245-1297	1.000000	77.1
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TGGCCCTTTATTAATCGGGG	ACGTTGGGTCTGTAAAGCAA	20	20	50.0	45.0	57.3	57.7	15.4	0.0	1.003460	56543	57625	1241-1293	1.000000	76.5
atpB-rbcL_p1	atpB-rbcL	1	CCTGGGGGAAAAGCTACATC	AACACCAGCTTTGAACCCAA	20	20	55.0	45.0	57.9	58.1	0.0	0.0	0.199291	60727	61516	952-955	1.000000	80.8
atpB-rbcL_p2	atpB-rbcL	2	CTGGGGGAAAAGCTACATCC	AACACCAGCTTTGAACCCAA	20	20	55.0	45.0	57.9	58.1	4.2	0.0	0.199291	60727	61516	951-954	1.000000	80.8
atpB-rbcL_p3	atpB-rbcL	3	GGGGGAAAAGCTACATCCAG	AACACCAGCTTTGAACCCAA	20	20	55.0	45.0	57.9	58.1	12.8	0.0	0.199291	60727	61516	949-952	1.000000	80.8
atpB-rbcL_p4	atpB-rbcL	4	AAGCTACATCCAGTACCGGA	AACACCAGCTTTGAACCCAA	20	20	50.0	45.0	57.8	58.1	9.5	0.0	0.295265	60727	61516	942-945	1.000000	80.1
atpB-rbcL_p5	atpB-rbcL	5	ATTTGAGCGATACGTCCCAG	AACACCAGCTTTGAACCCAA	20	20	50.0	45.0	57.8	58.1	0.0	0.0	0.365766	60727	61516	916-919	1.000000	79.6
accD-psaI_p1	accD-psaI	1	TTGATTCAATCGTACCGCGT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	58.0	58.0	4.9	26.9	0.027955	65499	66113	773-798	1.000000	82.2
accD-psaI_p2	accD-psaI	2	TTGATTCAATCGTACCGCGT	AAGAAGCCATTGCAATTGCC	20	20	45.0	45.0	58.0	57.9	4.9	26.8	0.104489	65499	66113	779-804	1.000000	81.6
accD-psaI_p3	accD-psaI	3	TCAATCGTACCGCGTAATCC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.9	58.0	4.9	26.9	0.151015	65499	66113	768-793	1.000000	81.2
accD-psaI_p4	accD-psaI	4	CAATCGTACCGCGTAATCCT	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.9	58.0	4.9	26.9	0.156826	65499	66113	767-792	1.000000	81.2
accD-psaI_p5	accD-psaI	5	TCAATCGTACCGCGTAATCC	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	57.9	57.9	4.9	26.8	0.227548	65499	66113	774-799	1.000000	80.6
petA-psbJ_p1	petA-psbJ	1	GTCTCTTGTTCTTCGTGGCA	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	58.1	57.6	0.0	4.1	0.499107	69309	70648	1455-1485	1.000000	77.0
petA-psbJ_p2	petA-psbJ	2	CGTGTCCAAGGTCTCTTGTT	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.8	57.6	5.6	4.1	0.605466	69309	70648	1465-1495	1.000000	76.2
petA-psbJ_p3	petA-psbJ	3	TGTTCTTCGTGGCATCTGTT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	57.7	57.6	0.0	4.1	0.619518	69309	70648	1449-1479	1.000000	76.1
petA-psbJ_p4	petA-psbJ	4	TTGTTCTTCGTGGCATCTGT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	57.7	57.6	0.0	4.1	0.619518	69309	70648	1450-1480	1.000000	76.1
petA-psbJ_p5	petA-psbJ	5	CGTTACGTGTCCAAGGTCTC	ATTCCGGATTGGGTTCATCC	20	20	55.0	50.0	58.3	57.6	0.0	4.1	0.670333	69309	70648	1470-1500	1.000000	75.7
rps3-rps19_p1	rps3-rps19	1	TACCAAGTCTGAAACCGAGC	ATAAACTTCCGGGGACATGC	20	20	50.0	50.0	57.5	57.9	0.0	0.0	0.503392	91340	92112	836-863	1.000000	79.6
rps3-rps19_p2	rps3-rps19	2	GTCTGAAACCGAGCGGATTT	ATAAACTTCCGGGGACATGC	20	20	50.0	50.0	58.6	57.9	16.8	0.0	0.613103	91340	92112	830-857	1.000000	78.8
rps3-rps19_p3	rps3-rps19	3	TGGTTGTGCAAACCAAAGAG	ATAAACTTCCGGGGACATGC	20	20	45.0	50.0	57.0	57.9	22.9	0.0	1.010273	91340	92112	874-901	1.000000	75.4
rps3-rps19_p4	rps3-rps19	4	TCTGAAACCGAGCGGATTTA	ATAAACTTCCGGGGACATGC	20	20	45.0	50.0	57.0	57.9	16.8	0.0	1.095356	91340	92112	829-856	1.000000	74.9
rps3-rps19_p5	rps3-rps19	5	GTCTGAAACCGAGCGGATT	ATAAACTTCCGGGGACATGC	19	20	52.6	50.0	57.9	57.9	16.8	0.0	1.191811	91340	92112	830-857	1.000000	74.1
ndhF-rpl32_p1	ndhF-rpl32	1	CGGGAAAAAGGAGAAGTCCC	TGCTACCCAATATGCCTTCT	20	20	55.0	45.0	58.2	56.3	0.0	0.0	1.901904	120595	121537	1117-1119	1.000000	65.4
ndhF-rpl32_p2	ndhF-rpl32	2	CGGGAAAAAGGAGAAGTCCC	TCAATGCTACCCAATATGCCT	20	21	55.0	42.9	58.2	57.1	0.0	0.0	2.059596	120595	121537	1121-1123	1.000000	64.1
ndhF-rpl32_p3	ndhF-rpl32	3	CGGGAAAAAGGAGAAGTCCC	TGCTACCCAATATGCCTTCTT	20	21	55.0	42.9	58.2	57.0	0.0	0.0	2.129057	120595	121537	1117-1119	1.000000	63.5
ndhF-rpl32_p4	ndhF-rpl32	4	CGGGAAAAAGGAGAAGTCCC	GCTACCCAATATGCCTTCTTCT	20	22	55.0	45.5	58.2	57.9	0.0	0.0	2.269610	120595	121537	1116-1118	1.000000	62.4
ndhF-rpl32_p5	ndhF-rpl32	5	CGGGAAAAAGGAGAAGTCCC	ACCCAATATGCCTTCTTCTTCC	20	22	55.0	45.5	58.2	58.1	0.0	0.0	2.274027	120595	121537	1113-1115	1.000000	62.4
ndhA_p1	ndhA	1	ACCCAGTTACCATAAGGAACA	GTCCCCCGGGTTTTATCAAT	21	20	42.9	50.0	56.3	57.6	0.0	21.8	3.195769	127802	130333	2753-2770	1.000000	55.3
ndhA_p2	ndhA	2	AACCCAGTTACCATAAGGAACA	GTCCCCCGGGTTTTATCAAT	22	20	40.9	50.0	57.0	57.6	0.0	21.8	3.452661	127802	130333	2754-2771	1.000000	53.3
ndhA_p3	ndhA	3	TGAACCCAGTTACCATAAGGA	GTCCCCCGGGTTTTATCAAT	21	20	42.9	50.0	56.0	57.6	0.0	21.8	3.479818	127802	130333	2756-2773	1.000000	53.1
ndhA_p4	ndhA	4	GAACCCAGTTACCATAAGGAACA	GTCCCCCGGGTTTTATCAAT	23	20	43.5	50.0	58.1	57.6	0.0	21.8	3.533624	127802	130333	2755-2772	1.000000	52.6
ndhA_p5	ndhA	5	TGAACCCAGTTACCATAAGGAAC	GTCCCCCGGGTTTTATCAAT	23	20	43.5	50.0	58.1	57.6	0.0	21.8	3.533624	127802	130333	2756-2773	1.000000	52.6
ycf1_p1	ycf1	1	TACCATACTGAAACGGCTGC	GCATTTTTGGGGTTCGATCG	20	20	50.0	50.0	58.0	58.1	0.0	9.4	0.110477	132823	132942	1237-1238	1.000000	81.8
ycf1_p2	ycf1	2	TACCATACTGAAACGGCTGC	ACGAACAACATCGAAGCAGA	20	20	50.0	45.0	58.0	57.9	0.0	3.5	0.167457	132823	132942	780-780	1.000000	81.6
ycf1_p3	ycf1	3	ACTGAAACGGCTGCCATTAT	GCATTTTTGGGGTTCGATCG	20	20	45.0	50.0	57.9	58.1	20.3	9.4	0.220705	132823	132942	1231-1232	1.000000	80.8
ycf1_p4	ycf1	4	ATACTGAAACGGCTGCCATT	GCATTTTTGGGGTTCGATCG	20	20	45.0	50.0	57.9	58.1	20.3	9.4	0.220705	132823	132942	1233-1234	1.000000	80.8
ycf1_p5	ycf1	5	TACCATACTGAAACGGCTGC	TGAACTGGCGTGATTGCTTA	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.195936	132823	132942	722-722	0.800000	70.5